CN1412163A - Preparation method of total saponin and flavone extract in notoginseng leaf - Google Patents
Preparation method of total saponin and flavone extract in notoginseng leaf Download PDFInfo
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- CN1412163A CN1412163A CN 02144841 CN02144841A CN1412163A CN 1412163 A CN1412163 A CN 1412163A CN 02144841 CN02144841 CN 02144841 CN 02144841 A CN02144841 A CN 02144841A CN 1412163 A CN1412163 A CN 1412163A
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Abstract
The preparation method of notoginseng leaf total saponin and flavone extract includes the following steps: decocting notoginseng leaf body adding water, combining filtrates obtained by decocting notoginseng leaf for several time, concentrating, filtering concentrate to obtain filtrate for stand-by; making the filtrate pass through the combined maroporous adsorption resin column, said combined maroporous adsorption resin can be divided into two kinds of macroporous adsorption resin, they are respectively mounted in upper and lower position of resin column, middle position is separated by means of defatted cotton, washing, removing washing liquor, using ethyl alcohol to make elution, cottecting eluant, recovering ethyl alcohol, concentrating and drying so as to obtain the invented products whose net content is above 50%.
Description
Technical field
The invention belongs to the extracts active ingredients field of crude drug.
Background technology
Sanchi Leaf is the dry cauline leaf of panax araliaceae plant Panax notoginseng (Burk.) F.H.chen, it contain with Radix Notoginseng in similar total saponins, content height in the flavones ingredient beguine that contains simultaneously, it is the ratio difference of monomer saponin in the total saponins, mostly be the dammarane type saponin(e of 20 (S)-protopanoxadiol types in the Sanchi Leaf greatly, Rb saponins content is higher, especially ginsenoside Rb
3The content height.And panoxadiol is suitable with triol type saponin content in the Radix Notoginseng.Folium Notoginseng total arasaponins has vasodilation equally, the effect of anticoagulant, the effect that also has anti-inflammatory, analgesia, anti-ageing, raise immunity simultaneously.
At present, the domestic colleague of the preparation method of saponin component also has report in the Sanchi Leaf, decocting or the extraction using alcohols of adopting more, extracting solution is with sherwood oil or ether defatting, and water liquid extracts with water-saturated n-butanol, or water liquid is concentrated into flowing soaking paste, add water-saturated n-butanol, heating is extracted, and divides then and gets n-butanol layer, propyl carbinol liquid reclaims solvent, promptly gets Folium Notoginseng total arasaponins.These methods have all adopted relatively large organic solvent, and are inflammable, explosive, the cost height, and the Folium Notoginseng total arasaponins powder appearance luster that makes is dark, and the net content of Folium Notoginseng total arasaponins is on the low side.
In addition, simultaneously extraction separation prepares saponin component and flavones ingredient in the Sanchi Leaf, reaches method more than 50% and make total saponins and total flavones net content in the Sanchi Leaf total effective parts, does not appear in the newspapers.
Summary of the invention
The invention provides the preparation method of total saponins and flavonoid extracts in a kind of Sanchi Leaf, is solvent with water, ethanol only, adopts adsorption resin method to prepare high purity saponin(e and flavonoid extracts in the Sanchi Leaf, and the concrete technical scheme that the present invention takes is:
(1) with Sanchi Leaf boiling 2～3 times, each 1～2 hour;
(2) filtrate with each time decoction gained merges, and concentrates;
(3) concentrated solution is filtered filtrate for later use;
(4) filtrate is crossed the associating macroporous adsorptive resins, this associating macroporous adsorbent resin is two kinds of macroporous adsorbent resins: flavones resin and D
101Macroporous resin is loaded on the upper and lower position of resin column respectively with certain proportioning, middlely separates with absorbent cotton, with 0%～40% washing with alcohol, discards washing lotion, with 40%～100% ethanol elution, collects elutriant;
(5) elutriant is reclaimed ethanol, concentrate, drying gets Folium Notoginseng total arasaponins and flavonoid extracts.
Be solvent with water and ethanol only in the preparation process of the present invention, reduce and pollute that can reuse for several times after the used resin regeneration, cost is low, total saponins and flavones net content in the Sanchi Leaf total effective parts that makes reach more than 50%.The extract of gained is used for the treatment of cardiovascular and cerebrovascular diseases, immunologic dysfunction, reducing blood-fat and pains and other diseases among the present invention.
Embodiment
Embodiment 1:
(1) with Sanchi Leaf boiling 3 times, each 2 hours;
(2) filtrate with three decoction gained merges, and relative density is 1.06 when being concentrated into 30 ℃;
(3) concentrated solution is filtered filtrate for later use;
(4) filtrate is crossed the associating macroporous adsorptive resins, this associating macroporous adsorbent resin is two kinds of macroporous adsorbent resins: flavones resin and D
101Macroporous resin is loaded on the upper and lower position of resin column respectively with 1: 2 proportioning, middlely separates with absorbent cotton, with 40% washing with alcohol, discards washing lotion, with 85% ethanol elution, collects elutriant;
(5) elutriant is reclaimed ethanol, concentrate, drying gets Folium Notoginseng total arasaponins and flavonoid extracts.
Embodiment 2:
(1) with Sanchi Leaf boiling 2 times, each 1 hour;
(2) filtrate with twice decoction gained merges, and relative density is 1.11 when being concentrated into 50 ℃;
(3) concentrated solution is filtered filtrate for later use;
(4) filtrate is crossed the associating macroporous adsorptive resins, this associating macroporous adsorbent resin is two kinds of macroporous adsorbent resins: flavones resin and D
101Macroporous resin is loaded on the upper and lower position of resin column respectively with 1: 3 proportioning, middlely separates with absorbent cotton, with 10% washing with alcohol, discards washing lotion, with 65% ethanol elution, collects elutriant;
(5) elutriant is reclaimed ethanol, concentrate, drying gets Folium Notoginseng total arasaponins and flavonoid extracts.
Embodiment 3:
(1) with Sanchi Leaf boiling 3 times, each 1.5 hours;
(2) filtrate with three decoction gained merges, and relative density is 1.06 when being concentrated into 80 ℃;
(3) concentrated solution is filtered filtrate for later use;
(4) filtrate is crossed the associating macroporous adsorptive resins, this associating macroporous adsorbent resin is two kinds of macroporous adsorbent resins: flavones resin and D
101Macroporous resin is loaded on the upper and lower position of resin column respectively with 2: 3 proportionings, middlely separates with absorbent cotton, with 12% washing with alcohol, discards washing lotion, with 95% ethanol elution, collects elutriant;
(5) elutriant is reclaimed ethanol, concentrate, drying gets Folium Notoginseng total arasaponins and flavonoid extracts.
Claims (3)
1, the preparation method of total saponins and flavonoid extracts in the Sanchi Leaf is characterized in that may further comprise the steps:
(1) with Sanchi Leaf boiling 2～3 times, each 1～2 hour;
(2) filtrate with each time decoction gained merges, and concentrates;
(3) concentrated solution is filtered filtrate for later use;
(4) filtrate is crossed the associating macroporous adsorptive resins, this associating macroporous adsorbent resin is two kinds of macroporous adsorbent resins are loaded on resin column respectively with certain proportioning upper and lower position, the centre separates with absorbent cotton, with 0%～40% washing with alcohol, discard washing lotion, with 40%～100% ethanol elution, collect elutriant;
(5) elutriant is reclaimed ethanol, concentrate, drying gets Folium Notoginseng total arasaponins and flavonoid extracts.
2, the preparation method of total saponins and flavonoid extracts in the Sanchi Leaf according to claim 1 is characterized in that: in (2) step during with solution concentration to 30 ℃～80 ℃ relative density be 1.01～1.20.
3, the preparation method of total saponins and flavonoid extracts in the Sanchi Leaf according to claim 1 is characterized in that: the associating adsorption resin column upper and lower part that filtrate is crossed in (4) step is respectively flavones resin and D
101Macroporous resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02144841 CN1200916C (en) | 2002-11-13 | 2002-11-13 | Preparation method of total saponin and flavone extract in notoginseng leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02144841 CN1200916C (en) | 2002-11-13 | 2002-11-13 | Preparation method of total saponin and flavone extract in notoginseng leaf |
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| Publication Number | Publication Date |
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| CN1412163A true CN1412163A (en) | 2003-04-23 |
| CN1200916C CN1200916C (en) | 2005-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 02144841 Expired - Lifetime CN1200916C (en) | 2002-11-13 | 2002-11-13 | Preparation method of total saponin and flavone extract in notoginseng leaf |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011072619A1 (en) | 2009-12-17 | 2011-06-23 | 天津天士力制药股份有限公司 | Traditional chinese drug comprising danshen extracts and sanqi extracts and use thereof |
| CN103462009A (en) * | 2013-09-04 | 2013-12-25 | 云南七丹药业股份有限公司 | Traditional Chinese medicine extract for assisting reduction of blood fat and preparation method thereof |
| CN105695284A (en) * | 2014-12-24 | 2016-06-22 | 广西大学 | Passion fruit vinegar with pseudo-ginseng and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102274260B (en) * | 2011-08-09 | 2013-04-10 | 北京中海康医药科技发展有限公司 | Medicinal composition of notoginseng root extract and preparation method thereof |
-
2002
- 2002-11-13 CN CN 02144841 patent/CN1200916C/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011072619A1 (en) | 2009-12-17 | 2011-06-23 | 天津天士力制药股份有限公司 | Traditional chinese drug comprising danshen extracts and sanqi extracts and use thereof |
| CN103462009A (en) * | 2013-09-04 | 2013-12-25 | 云南七丹药业股份有限公司 | Traditional Chinese medicine extract for assisting reduction of blood fat and preparation method thereof |
| CN105695284A (en) * | 2014-12-24 | 2016-06-22 | 广西大学 | Passion fruit vinegar with pseudo-ginseng and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN1200916C (en) | 2005-05-11 |
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