CN1410069A - Broad spectrum anti bacterial traditional Chinese medicine and its preparation method - Google Patents
Broad spectrum anti bacterial traditional Chinese medicine and its preparation method Download PDFInfo
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- CN1410069A CN1410069A CN02148309A CN02148309A CN1410069A CN 1410069 A CN1410069 A CN 1410069A CN 02148309 A CN02148309 A CN 02148309A CN 02148309 A CN02148309 A CN 02148309A CN 1410069 A CN1410069 A CN 1410069A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
A Chinese medicine preparation with broad antibacterial spectrum contains tyrosol (2-6%), 3,4-dihydroxyphenyl ethanol (5-10%), syringopicroside (10-20%) and auxiliaries (the rest). It is suitable for treating the bacteria or virus infective diseases, such as bacillary dysentery, upper respiratory tract infection, acute or chronic bronchitis, etc.. Its advantages are high curative effect, and no toxic by-effect.
Description
Affiliated field: the present invention relates to Chinese medicine preparation of a kind of broad-spectrum antiseptic and preparation method thereof.
Background technology: antibacterial and disease of viral infection comprise wide, the sickness rate height, complication is many, seriously endanger the people's health and lives, chemobiotic is used frequently clinically, is second-biggest-in-the-world drug market based on the anti-infective market of antibiotics sulfonamides, and annual sales amount is 2,050,000 dollars, wherein 80% is antimicrobial drug, and all the other 20% are antifungal agent and antiviral agents.Since the fifties, because antibiotic medicine extensive use, cause antibacterial to morph, the multiple antibiotics effect of original good antibacterial action is significantly reduced, clinical using dosage increases again and again, more strengthened the potential threat to human body, therefore seek new anti-infective in Chinese medicine is the focus of domestic and international new drug development always thereupon.Chinese medicine has special advantages in this type of disease of treatment.The one, the natural product toxic and side effects is little, and the 2nd, multiple composition is collaborative to be used as medicine, and can produce the incomparable therapeutic effect of single chemical constituent.But it is many that traditional Chinese medicine preparation also is difficult to break away from impurity, and dose is big, the indefinite many shortcomings of effective ingredient.
Summary of the invention: the purpose of this invention is to provide a kind of drug resistance, anaphylaxis, side effect that had both changed antibiotic medicine and brought, it is fewer but better to reach dose again, and effective ingredient is clear and definite, Chinese medicine preparation of the broad-spectrum antiseptic that curative effect is clear and definite and preparation method thereof.The objective of the invention is to be achieved through the following technical solutions: the present invention is a kind of Chinese medicine preparation of broad-spectrum antiseptic, it is made up of following active ingredient, the effective range of its percentage by weight is: butyl alcohol 5～10%, 3,4-dihydroxyphenyl ethanol 2～6%, syringopicroside 15～25%, organic acid 5～19%, glycoside 40～50%, all the other are adjuvant.Preparation method provided by the invention is: A: get Folium Caryophylli 100kg, the decocting that adds 6～12 times of amounts according to volume ratio boils twice, merge decocting liquid, concentrating under reduced pressure relative density to 1.0～1.2 (80 ℃ of heat are surveyed), add 85～90% ethanol according to volume ratio, concentration of alcohol is added according to volume ratio reach 55～70%, static 24 hours, B: get supernatant, decompression recycling ethanol, to fluid extract proportion be 1: 1～1.2g/mL, this extractum is placed on the macroporous resin column, the macroporous adsorptive resins blade diameter length ratio is 1: 6～8, weight resin and Folium Caryophylli crude drug weight ratio are 1: 1～1.2, C: remove impurity with 8～12 times of water elutions, to water lotion colourless till, add 50～65% with 10～12 times of amounts according to volume ratio then, the ethanol water eluting is collected 50～65% ethanol elution, decompression recycling ethanol to 1000～1500L, D: equimultiple ethyl acetate extraction 3～6 times, reclaim ethyl acetate to doing, use the water for injection heating for dissolving, behind the employing 0.6-0.8nm filtering with microporous membrane, transfer PH=6.5～8, add sodium sulfite, E: lyophilization, sterilization, the powder pin is made in packing.
Antibacterial action.Extracorporeal bacteria inhibitor test: adopt test tube doubly to measure dilution method, with the ordinary broth is culture medium, 22 kinds of strains such as dysentery bacterium, staphylococcus aureus, Bacillus typhi have been carried out extracorporeal bacteria inhibitor test research, the result shows: the water decoction of (1) Folium Caryophylli is to common pyogenic infection staphylococcus aureus and Staphylococcus albus, to Bacillus proteus and the bacillus pyocyaneus that causes secondary infection, to various (type) dysentery bacterium, Bacillus typhi and Salmonella paratyphi, to cause alimentary toxicosis bacillus typhi murium, Salmonella etc. tangible antibacterial action is arranged.(2) to the staphylococcus aureus and the various dysentery bacterium of anti-sulfanilamide, the leaf of Syringa oblata Lindl. water decoction has tangible antibacterial action.
Bacteriostatic test in the body: mice infected such as staphylococcus aureus, dysentery bacterium in the body is had significant protective effect, and ED50 is 4.1 ± 5.9g/kg.
Antivirus action: adenovirus (Afv3, Afv7), parainfluenza virus (I, II, III), respiratory syncytial virus (RSV) and Coxsackie virus (CoxBl, CoxB2, CoxB3, CoxB4, CoxB5, CoxB6) institute cytopathogenic effect are had obvious inhibitory action.Its potency is better than Radix Isatidis granules.
Antiinflammatory action: the mouse tissue swelling due to xylol induced mice auricular concha inflammation and the carrageenin all has remarkable inhibitory action, has similar effect with dexamethasone.
The humoral immune function of enhancing body: contrast with test of hemolytic plaque and hemolysin mensuration, the Folium Caryophylli water decoction has the effect that promotes that mouse spleen hemolytic plaque forms and specific antibody one hemolysin forms, can increase simultaneously B cell percentage ratio in the animal peripheral blood, illustrate that this medical instrument has the function of the humoral immunity of organism of stimulation.Inferring that this medicine treatment infectious disease strengthens the humoral immune function of body in direct antibacterial action, is the rehabilitation that helps body.
Protective effect to experimental hepatic injury: GSH content due to the acetaminophen is exhausted having resistance inhibitor action, thereby hepatocyte injury is had protective effect.
Toxicity test: 1 acute toxicity test: the clever powder pin of wide inflammation has been carried out animal toxicity test, mouse stomach administration LD
50=183.0 ± 0.47mg/kg.2 subacute toxicity tests: with the clever powder pin of wide inflammation respectively to normal saline matched group, high, medium and low dose drug group continuous irrigation stomach 14 days, peripheral hemogram and matched group relatively there is not significant difference, do not change having significantly in body weight, liver weight, the heart, liver, kidney, intestinal, the gastroscope inspection morphology, none is only dead for rat.
The present invention observes through hospital clinical, to 314 routine bacillary dysentery cure rates is more than 90.5%, to 223 example other bacterial infection diseases (acute suppurative tonsillitis, acute bronchitis, chronic bronchitis, obstructive emphysema, merging pulmonary infection, acute gastroenteritis, mastitis) effective percentage is more than 92.0%, is 79.0% to the invalid case effective percentage of 30 routine Western medicine antibiotic therapies.And bring down a fever fast, remove that colic symptoms is fast, the tenesmus symptom eliminates fast, stool character and recovers normal fast.Do not find toxicity, side effect.Treatment acute icterohepatitisshock, infectious hepatitis 178 examples obtain obviously.
The present invention has the effect of treatment antibacterial and disease of viral infection, is used for diseases such as bacillary dysentery, upper respiratory tract infection disease, acute and chronic bronchitis, acute gastroenteritis, acute suppurative tonsillitis, obstructive emphysema, chronic pharyngitis, merging pulmonary infection, mastitis, acute icterohepatitis, infectious hepatitis.
The present invention can adopt Folium Caryophylli as the directed raw material of pharmacy: Folium Caryophylli is to collect antibiotic simply and antiviral is the Northeast's native drug all over the body, derives from the dried leaves of Oleaceae genus syringa Syringa oblata Lindl., Korea Flos Caryophylli or foreign Flos Caryophylli.The effective site of Folium Caryophylli comprises bacillary dysentery, acute gastroenteritis, acute suppurative tonsillitis, acute bronchitis, chronic bronchitis, obstructive emphysema, merging pulmonary infection, mastitis, acute icterohepatitisshock, infectious hepatitis determined curative effect to antibacterial and disease of viral infection.By advanced person's preparation technology, exploitation is the raw material Chinese medicine preparation with the Folium Caryophylli, the drug resistance, anaphylaxis, the side effect that had both changed antibiotic medicine and brought, and it is fewer but better to reach dose again, and effective ingredient is clear and definite, the purpose that curative effect is clear and definite.
Description of drawings:
Fig. 1 is a process frame chart of the present invention
The specific embodiment:
The present invention is made up of following active ingredient, and the effective range of its percentage by weight is: butyl alcohol 5～10%, 3, and 4-dihydroxyphenyl ethanol 2～6%, syringopicroside 15～25%, organic acid 5～19%, glycoside 40～50%, all the other are adjuvant.In the present embodiment: butyl alcohol is 6.4%, 3, and the 4-dihydroxyphenyl ethanol is 3.65%, and syringopicroside is 20.6%.Adjuvant can be: mannitol, can be used for regulating osmotic pressure, and adjuvant can also be a sodium sulfite, it is an antioxidant.
Organic acid comprises organic acid compositions such as ferulic acid, caffeic acid, protocatechuic acid, Naringeninic acid, and the glycoside composition comprises: 25 kinds of glycoside compositions of methoxyconiferyl alcohol A, B, C, syringopicroside A, B, C, oleuropein, foreign Flos Caryophylli phenolic glycoside.In the positive male and mountain, Japan scholar chrysanthemum ground people such as foreign son from foreign Flos Caryophylli and syringa reticulata var mandshurica leaf except that isolating butyl alcohol, 3, outside the 4-dihydroxyphenyl ethanol, also isolate organic acid compositions such as oleanolic acid (Oleanolic acid), ursolic acid (Ursolic acid), Crataegolic acid (Maslinicacid), ferulic acid (Ferulic acid), caffeic acid (Caffeic acid) and cinnamic acid.Britain scholar Heimot ripperger in 1977 separates from leaf of Syringa vulgaris L. and obtains two kinds of alkaloids, is respectively Jamindine and Jaminine.There is the scholar that Oleaceae Genus Syringa flavones ingredient is studied again, finds that kaempferol, Quercetin and they are its common flavone compounds at 3,7 glucoside and rhamnoside.The volatile oil component of trace element in cupreol, daucosterol, mannitol, starch, sucrose, moisture and the Flos Caryophylli and this platymiscium Henan Sytingamicrophylla also appears in the newspapers.
As shown in Figure 1, a kind of broad-spectrum antiseptic Chinese medicine preparation preparation method of the present invention is: A: get Folium Caryophylli 100kg, the decocting that adds 6～12 times of amounts according to volume ratio boils twice, merge decocting liquid, concentrating under reduced pressure relative density to 1.0～1.2 (80 ℃ of heat are surveyed), add 85～90% ethanol according to volume ratio, concentration of alcohol is added according to volume ratio reach 55～70%, static 24 hours, B: get supernatant, decompression recycling ethanol, to fluid extract proportion be 1: 1～1.2g/mL, this extractum is placed on the macroporous resin column, and the macroporous adsorptive resins blade diameter length ratio is 1: 6～8, and weight resin and Folium Caryophylli crude drug weight ratio are 1: 1～1.2, C: remove impurity with 8～12 times of water elutions, to water lotion colourless till, add 50～65% with 10～12 times of amounts according to volume ratio then, the ethanol water eluting, collect 50～65% ethanol elution, decompression recycling ethanol to 1000～1500L, D: equimultiple ethyl acetate extraction 3～6 times, reclaim ethyl acetate to doing, use the water for injection heating for dissolving, after adopting the 0.6-0.8nm filtering with microporous membrane, transfer PH=6.5～8, add sodium sulfite, E: lyophilization, sterilization, the powder pin is made in packing.External bacteriostatic experiment proves: 3, and 4-dihydroxyphenyl ethanol bacteriostasis is strong; Butyl alcohol, 3, compositions such as 4-resorcylic acid, Naringeninic acid also have to a certain degree bacteriostasis.Heilongjiang University of Chinese Medicine studies show that bacteriostasis is strong in the syringopicroside A body, and the content of syringopicroside A accounts for 1.1 ‰ of leaf.
Claims (2)
1, a kind of Chinese medicine preparation of broad-spectrum antiseptic is characterized in that: it is made up of following active ingredient, and the effective range of its percentage by weight is: butyl alcohol 5～10%, 3,4-dihydroxyphenyl ethanol 2～6%, syringopicroside 15～25%, organic acid 5～19%, glycoside 40～50%, all the other are adjuvant.
2, preparation method according to a kind of broad-spectrum antiseptic Chinese medicine preparation that claim 1 provided, it is characterized in that: A: get Folium Caryophylli 100kg, the decocting that adds 6～12 times of amounts according to volume ratio boils twice, merge decocting liquid, concentrating under reduced pressure relative density to 1.0～1.2 (80 ℃ of heat are surveyed), add 85～90% ethanol according to volume ratio, concentration of alcohol is added according to volume ratio reach 55～70%, static 24 hours, B: get supernatant, decompression recycling ethanol, to fluid extract proportion be 1: 1～1.2g/mL, this extractum is placed on the macroporous resin column, and the macroporous adsorptive resins blade diameter length ratio is 1: 6～8, and weight resin and Folium Caryophylli crude drug weight ratio are 1: 1～1.2, C: remove impurity with 8～12 times of water elutions, to water lotion colourless till, add 50～65% with 10～12 times of amounts according to volume ratio then, the ethanol water eluting, collect 50～65% ethanol elution, decompression recycling ethanol to 1000～1500L, D: equimultiple ethyl acetate extraction 3～6 times, reclaim ethyl acetate to doing, use the water for injection heating for dissolving, after adopting the 0.6-0.8nm filtering with microporous membrane, transfer PH=6.5～8, add sodium sulfite, E: lyophilization, sterilization, the powder pin is made in packing.
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| CN02148309A CN1410069A (en) | 2002-10-30 | 2002-10-30 | Broad spectrum anti bacterial traditional Chinese medicine and its preparation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283898A (en) * | 2011-04-13 | 2011-12-21 | 沈阳药科大学 | Flos caryophyllata leaf extract and preparation method thereof |
| CN102475711A (en) * | 2010-11-30 | 2012-05-30 | 李永吉 | Novel application of syringopicroside in preparation of medicines |
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2002
- 2002-10-30 CN CN02148309A patent/CN1410069A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102475711A (en) * | 2010-11-30 | 2012-05-30 | 李永吉 | Novel application of syringopicroside in preparation of medicines |
| CN102475711B (en) * | 2010-11-30 | 2013-11-13 | 李永吉 | Novel application of syringopicroside in preparation of medicines |
| CN102283898A (en) * | 2011-04-13 | 2011-12-21 | 沈阳药科大学 | Flos caryophyllata leaf extract and preparation method thereof |
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