CN1402968A - Selective breeding process of hybrid rice with greening leaf color label carried in its seedling stage - Google Patents
Selective breeding process of hybrid rice with greening leaf color label carried in its seedling stage Download PDFInfo
- Publication number
- CN1402968A CN1402968A CN 02137544 CN02137544A CN1402968A CN 1402968 A CN1402968 A CN 1402968A CN 02137544 CN02137544 CN 02137544 CN 02137544 A CN02137544 A CN 02137544A CN 1402968 A CN1402968 A CN 1402968A
- Authority
- CN
- China
- Prior art keywords
- hybrid rice
- line
- leaf
- seed
- leaf color
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention features that by using two-line hybrid rice sterile line, three-line hybrid rice maintainer line and hybrid rice restoring line as initiative material and through physical and chemical induction mutation of seed, screening of greening leaf color label mutation line carrying stable seedling stage expression and controlled by mono-recessive gene, linear selection or hybridization or backcrossing, and identifying agricultural characteristic, quality characteristic, disease resistance, breeding features and combining ability to breed hybrid rice with greening leaf color label. The said method can monitor the seed quality of hybrid rice and identify fast the seed purity of hybrid rice sterile line and hybrid seed F1 to provide high-purity hybrid rice seed.
Description
Technical field
The present invention relates to the selection of a kind of greening leaf color label carried in its seedling stage hybrid rice.
Background technology
In the double-linear hybrid rice seeds production process, photo-thermo-sensitive genic male sterile line is subject to the variation of unusual temperature and causes fertility answer in various degree, influences hybrid F
1Seed purity.Series of three-series hybrid rice male sterile lines such as Long Tefu A and II32A have hybrid seed yield height, characteristics that the combo advantage is strong, and the production application area enlarges just year by year, but its sterility is thorough inadequately, also difficultly thoroughly solve even if purify by heredity.For this reason, although each research unit and seed operating unit cost great amount of manpower and material resources and financial resources are identified hybrid purity, almost still have the dispute that causes because of purity problem every year.But the leaf color marker is a kind of with the external label of naked eyes with regard to Direct Recognition.If it is combined with hybrid rice, as long as true and false hybrid is according to the notable difference of leaf look, just can seedling stage by artificial auxiliary reject or the competitive ability of growing because of it a little less than natural selection, thereby guarantee true hybrid F
1Purity.
Summary of the invention
The object of the present invention is to provide the selection of greening leaf color label carried in its seedling stage hybrid rice, realize quick, easy, intuitively identify the seed purity of hybrid rice, for production provides " relieved " seed.
For achieving the above object, the selection of greening leaf color label carried in its seedling stage hybrid rice of the present invention is characterized in that may further comprise the steps:
1) dry seeds of getting double-linear hybrid rice male sterile line that moisture content is 13%-13.5% or series of three-series hybrid rice maintenance line or hybrid rice restorer line carries out mutagenic treatment as starting material with physics or chemical mutagen;
2) sowing mutagenesis (M in the present age
0) seed, by bulk method or an individual plant method results mutagenesis generation (M
1) seed of plant, be method plantation mutagenesis two generations (M by bulk method or strain
2), grow to two leaves wholeheartedly the time with naked eyes screening leaf variegation body, the numbering variant also moves to a place separately, selects and remain promptly to begin the variation individual plant that changes green with the growth of the 4th leaf;
3) from mutagenesis three generations (M
3) beginning, test and estimate between year at interval sowing, multiple spot under light temperature culturing room, the field natural conditions respectively, carry out light temperature, the evaluation of ecological equistability that leaf look mutant character is expressed, the leaf look mutantion line of screening environment non-sensitive type;
4) mutantion line of double-linear hybrid rice male sterile line is carried out economical character, quality trait, disease resistance, fertility feature and coordinate force and observe, the final two-line sterile line that gets greening leaf color label carried in its seedling stage.
To the series of three-series hybrid rice male sterile line, with the mutantion line of maintenance line hybridize, backcross transformation, quality trait, disease resistance, fertility identify and survey extensive combo, three of final greening leaf color label carried in its seedling stage is a male sterile line.
The mutantion line of hybrid rice restorer line is carried out economical character, quality trait, disease resistance and coordinate force observe, the final hybrid rice restorer line that gets greening leaf color label carried in its seedling stage.
In the said method, the mutagen that physical mutagenesis is handled are that cobalt is penetrated or caesium-137 gamma-rays for cobalt-60 γ, and the dosage of radiation is 200～400Gy.The mutagen that mutagenesis is handled are sodium azide (NaN3) or ethylmethane sulfonate (EMS), and wherein the dosage of sodium azide processing is 0.5mMh～4.0mMh, and the dosage that ethylmethane sulfonate is handled is 0.025Mh～0.2Mh.
The hybrid rice of the greening leaf color label carried in its seedling stage that the employing said method obtains has following feature: the 1-3 leaf shows complete albefaction, the albefaction of leaf periphery, yellow fully, the yellow of leaf periphery, yellow green, tiger spot striped, emerald green, begin to change green from the 4th leaf, the 6-7 leaf returns to normal green.
The greening leaf color label carried in its seedling stage hybrid rice that obtains with selection of the present invention is as the parent, but by hybridization, backcross or leaf color marker hybrid rice similarly is with in the somaclonal variation seed selection.
The greening leaf color label carried in its seedling stage hybrid rice that obtains with selection of the present invention can be used as the evaluation of hybrid rice seeds purity, auxiliary impurity elimination and row's vacation automatically.Be the hybrid rice seed that use in the land for growing field crops,, keep normal green rice shoot, just can plant the high-purity hybrid rice, improve rice yield as long as reject or the automatic rice shoot of getting rid of band leaf color marker in the manually auxiliary phase of seedling.By evaluation sterile line of hybridized rice directly perceived, the seed purity of the first generation of hybrid has solved its purity and has identified hysteresis, time length, manpower, the problem that financial resources consumption is big.
Advantage of the present invention is:
1, by this selection, make dissimilar hybrid rice with going up greening leaf color label easy to identify, brand-new hybrid rice production link seed quality monitoring system will be formed, the realization indoor and outdoor is identified the seed purity of hybrid rice fast, easily, efficiently solves the problem that its purity qualification time lags behind the seed sales behavior.
2, the leaf color marker of green changing type does not influence the hybrid rice growth of later stage, but can conveniently implement manually to assist rejecting or utilize self-sow to compete the to a certain degree automatic vacation of arranging in the seedling phase, guarantees that it is " relieved " high-purity paddy rice that seedling is used in the land for growing field crops.
3, seed operating division to the seed of producing this year, passes through sampling, according to hybrid F when purchase
1The leaf color marker determine the seed purity of hybrid rice that pay at the production family, can solve the responsibility and right dispute that produces because of quality problems in the seed operation.
Embodiment
Below further specify the present invention by instantiation.
Embodiment 1:
Physical mutagenesis double-linear hybrid rice male sterile line is trained short 64S seed selection " Jade Hare S ":
The two-line sterile line of getting water content 13.5% is trained the dry seeds of short 64S, handles with the gamma-ray irradiation of cobalt-60, and exposure dose is 300Gy.Live M
0Seed mixes and receives M
1The seed of plant.Mixed planting M
2Colony, when growth of seedling to two leaf wholeheartedly the time, the variant that screening can the albefaction of clear identification blade is gathered in the crops the 4th leaf by individual plant and is begun the variant seed that changes green.Stability, the leaf look of observing the albefaction proterties change variation after green, with growth and the control area of white proterties plant, different regions and variation of temperature, fertility feature and Analysis of Combining Ability, through repeated screening, superseded, finally selecting 1～3 leaf phase of seedling phase has complete albefaction mark, change green behind 4 leaves gradually, the 6-7 leaf phase transfers the new two-line sterile line " Jade Hare S " of normal green to.
Embodiment 2:
The male sterile line of mutagenesis maintainer line of hybrid rice II-32B seed selection band yellow green changing type mark:
Getting moisture content and be 13.5% maintainer line of hybrid rice II-32B dry seeds is starting material, and the room temperature seed soaking was handled with chemical mutagen under 25 ℃ after 16 hours, and the dosage of processing is 2mMh.Live M
0Seed mixes and receives M
1The seed of plant.Mixed planting M
2Colony, when growth of seedling to two leaf wholeheartedly the time, the variant that screening can the yellow of clear identification blade is gathered in the crops the 4th leaf by individual plant and is begun the variant seed that changes green.Carry out temperature and photoperiod sensitivity by light temperature culturing room and identify, reject responsive type, the leaf look mutant of the non-sensitive type of selecting and remain, and respectively in the different location to M
3Non-sensitive type leaf look mutant carries out interval sowing and multiple spot evaluation test under the natural conditions.M
4Carry out leaf look mutant different ecological Adaptability Evaluation, stability and genetic development research between year.Through above-mentioned multiple evaluation and evaluation, filter out that overall proterties is similar to former parent II-32B, yellow mark stably express mutantion line.Thereafter, be the parent with this type of sudden change, with the male sterile line of corresponding male sterile line II-32A hybridization, backcross breeding band yellow green changing type mark.Carry out the economical character, attributional analysis, disease resistance, fertility feature, coordinate force of this type of male sterile line and outcrossing seed-setting rate and observe and identify, final seed selection product are of fine quality, disease-resistant, the series of three-series hybrid rice male sterile line of stable fertility, coordinate force is good and outcrossing seed-setting rate is high band yellow green changing type mark.
Embodiment 3
The blade periphery albefaction green changing type mark paddy rice that obtains with selection of the present invention is the parent, by hybridization, backcross transformation blade tape label sterile line of hybridized rice:
With blade periphery albefaction green changing type mark paddy rice is leaf color marker donor, and three is maintenance line Zhenshan 97B hybridization, and artificial pollination obtains hybrid F
1Plantation hybrid F
1, when blooming and Zhenshan 97B backcross, obtain BC
1Plantation hybrid BC
1, mix and receive BC
1The seed of plant, mixed planting BC
1F
2Colony, when growth of seedling to two leaf wholeheartedly the time, screening also keeps the albefaction of blade periphery, can change green individual plant, backcrosses acquisition BC when blooming with Zhenshan 97B
2F
2So repeatedly, from BC
5F
2Filter out the maintenance line that overall characteristic is similar to former parent's Zhenshan 97B, be with blade periphery albefaction green changing type mark.Thereafter, be the parent with this type of maintenance, with the male sterile line of corresponding male sterile line Zhenshan 97a hybridization, backcross breeding band blade periphery albefaction green changing type mark.Carry out economical character, attributional analysis, disease resistance, fertility feature, coordinate force and the outcrossing seed-setting rate of this type of male sterile line and observe and evaluation the male sterile line (maintenance line) of final seed selection band blade periphery albefaction green changing type mark.
Embodiment 4
The Rapid identification of double-linear hybrid rice seeds purity and auxiliary impurity elimination:
Double-linear hybrid rice male sterile line Jade Hare S hybridization with band albefaction leaf color marker is maternal, is male parent with the hybrid rice restorer line M9311 of yellow greening leaf color label, the production of preparing hybrid seed and breeding.To the hybrid that produces, in indoor germination, in the seedling 1-3 leaf phase, if be found to be the male sterile line Jade Hare S seed that the albefaction seedling is then replied for fertility, if being found to be etiolated seedling is the M9311 seed for recovering then, with this ratio that mixes that calculates male sterile line in the hybrid rice hybrid intuitively, fast and recover system, determine the seed purity of hybrid.Utilize the hybrid rice seed period of the day from 11 p.m. to 1 a.m of Jade Hare S preparation in peasant's production, when seedling growth arrives the 1-3 leaf, as long as observe the seedling growth situation in rice seedling bed, finding has albefaction or yellow rice shoot in the rice seedling bed, rejecting immediately or allowing it pass through growth competes dead automatically, to guarantee land for growing field crops hybrid rice purity, ensure the hybrid rice high yield.During Jade Hare S breeding, the breeding field is purebred sub-seedling with band albefaction mark rice shoot, and the rice shoot of other color and luster is the accessory seed seedling, all rejects, and breeds highly purified Jade Hare S seed with this.When the Jade Hare S and the M9311 production of hybrid seeds, in the male sterile line Jade Hare S rice shoot, be pure Jade Hare S seed seedling with band albefaction mark rice shoot, the rice shoot of other color and luster is assorted strain, all rejects; Recovery is in the M9311 rice shoot, is pure M9311 seed seedling with band yellow mark rice shoot, and the rice shoot of other color and luster is assorted strain, all rejects.Prepare high-purity double-line hybrid rice with this.
Claims (6)
1. the selection of greening leaf color label carried in its seedling stage hybrid rice is characterized in that may further comprise the steps:
1) dry seeds of getting double-linear hybrid rice male sterile line that moisture content is 13%-13.5% or series of three-series hybrid rice maintenance line or hybrid rice restorer line carries out mutagenic treatment as starting material with physics or chemical mutagen;
2) the sowing mutagenesis present age (M0) seed, seed by bulk method or an individual plant method results mutagenesis generation (M1) plant, by bulk method or strain is method plantation mutagenesis two generations (M2), grow to two leaves wholeheartedly the time with naked eyes screening leaf variegation body, numbering variant and move to a place separately, selecting and remain promptly begins the variation individual plant that changes green with the growth of the 4th leaf;
3) from mutagenesis three generations (M3) beginning, test and estimate between year at interval sowing, multiple spot under light temperature culturing room, the field natural conditions respectively, carry out light temperature, the evaluation of ecological equistability that leaf look mutant character is expressed, the leaf look mutantion line of screening environment non-sensitive type;
4) mutantion line of double-linear hybrid rice male sterile line is carried out economical character, quality trait, disease resistance, fertility feature and coordinate force and observe, the final two-line sterile line that gets greening leaf color label carried in its seedling stage.
To the series of three-series hybrid rice male sterile line, with the mutantion line of maintenance line hybridize, backcross transformation, quality trait, disease resistance, fertility identify and survey extensive combo, three of final greening leaf color label carried in its seedling stage is a male sterile line.
The mutantion line of hybrid rice restorer line is carried out economical character, quality trait, disease resistance and coordinate force observe, the final hybrid rice restorer line that gets greening leaf color label carried in its seedling stage.
2. by the selection of the described greening leaf color label carried in its seedling stage hybrid rice of claim 1, it is characterized in that the mutagen that physical mutagenesis is handled are that cobalt-60 γ penetrates or caesium-137 gamma-rays, the dosage of radiation is 200～400Gy.
3. press the selection of the described greening leaf color label carried in its seedling stage hybrid rice of claim 1, it is characterized in that the chemical agent that mutagenesis is handled is sodium azide or ethylmethane sulfonate, wherein the dosage of sodium azide processing is 0.5mMh～4.0mMh, and the dosage that ethylmethane sulfonate is handled is 0.025Mh～0.2Mh.
4. press the selection of the described greening leaf color label carried in its seedling stage hybrid rice of claim 1, the hybrid rice that it is characterized in that the seed selection gained has following feature: the 1-3 leaf shows complete albefaction, the albefaction of leaf periphery, yellow fully, the yellow of leaf periphery, yellow green, tiger spot, striped, emerald green, begin to change green from the 4th leaf, the 6-7 leaf returns to normal green.
5. the greening leaf color label carried in its seedling stage hybrid rice that selection according to claim 1 obtains is characterized in that similarly being with leaf color marker hybrid rice as parent's seed selection.
6. the greening leaf color label carried in its seedling stage hybrid rice that obtains according to claim 1 and 5 described selections is characterized in that the evaluation as hybrid rice seeds purity, auxiliary impurity elimination and row is false automatically.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021375445A CN1164164C (en) | 2002-10-21 | 2002-10-21 | Selective breeding process of hybrid rice with greening leaf color label carried in its seedling stage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021375445A CN1164164C (en) | 2002-10-21 | 2002-10-21 | Selective breeding process of hybrid rice with greening leaf color label carried in its seedling stage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1402968A true CN1402968A (en) | 2003-03-19 |
| CN1164164C CN1164164C (en) | 2004-09-01 |
Family
ID=4749028
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021375445A Expired - Fee Related CN1164164C (en) | 2002-10-21 | 2002-10-21 | Selective breeding process of hybrid rice with greening leaf color label carried in its seedling stage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1164164C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405809A (en) * | 2011-08-17 | 2012-04-11 | 江西现代种业有限责任公司 | Etiolated two-line sterile line breeding method and use thereof |
| CN103444513A (en) * | 2013-08-14 | 2013-12-18 | 江苏丘陵地区镇江农业科学研究所 | Breeding method of three-line sterile line of indica type rice with high combining ability |
| CN104054571A (en) * | 2014-06-26 | 2014-09-24 | 浙江大学 | Breeding method of hybrid rice three-line sterile line with leaves carrying white midribs |
| CN104054570A (en) * | 2014-06-26 | 2014-09-24 | 浙江大学 | Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs |
| CN106636425A (en) * | 2017-01-12 | 2017-05-10 | 广西大学 | Rice purple leaf gene PL1 molecular marker, identification method thereof and application |
| CN107593430A (en) * | 2017-10-25 | 2018-01-19 | 湖南杂交水稻研究中心 | A kind of hybrid rice variety seed selection of suitable miscegenation production of hybrid seeds and seed production methods |
| CN111543312A (en) * | 2020-06-09 | 2020-08-18 | 中国热带农业科学院热带作物品种资源研究所 | Breeding method of two-line sterile line of greening new-leaf etiolation hybrid rice |
| CN116144674A (en) * | 2023-03-14 | 2023-05-23 | 西南大学 | Rice albino-transgenic green leaf mutant gene TWINKLE and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318551A (en) * | 2011-08-17 | 2012-01-18 | 江西现代种业有限责任公司 | Breeding method for hybrid rice three-line male sterile line with recessive marker and application thereof |
-
2002
- 2002-10-21 CN CNB021375445A patent/CN1164164C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405809A (en) * | 2011-08-17 | 2012-04-11 | 江西现代种业有限责任公司 | Etiolated two-line sterile line breeding method and use thereof |
| CN102405809B (en) * | 2011-08-17 | 2013-04-24 | 江西现代种业有限责任公司 | Etiolated two-line sterile line breeding method and use thereof for rice |
| CN103444513A (en) * | 2013-08-14 | 2013-12-18 | 江苏丘陵地区镇江农业科学研究所 | Breeding method of three-line sterile line of indica type rice with high combining ability |
| CN104054570B (en) * | 2014-06-26 | 2015-07-29 | 浙江大学 | A kind of blade carries the selection of the hybrid rice two-line sterile line of White midrib |
| CN104054570A (en) * | 2014-06-26 | 2014-09-24 | 浙江大学 | Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs |
| CN104054571B (en) * | 2014-06-26 | 2015-07-29 | 浙江大学 | A kind of blade carries the selection of the hybrid rice CMS line of White midrib |
| CN104054571A (en) * | 2014-06-26 | 2014-09-24 | 浙江大学 | Breeding method of hybrid rice three-line sterile line with leaves carrying white midribs |
| CN106636425A (en) * | 2017-01-12 | 2017-05-10 | 广西大学 | Rice purple leaf gene PL1 molecular marker, identification method thereof and application |
| CN106636425B (en) * | 2017-01-12 | 2020-07-10 | 广西大学 | Molecular marker of rice purple leaf gene P L1 and identification method and application thereof |
| CN107593430A (en) * | 2017-10-25 | 2018-01-19 | 湖南杂交水稻研究中心 | A kind of hybrid rice variety seed selection of suitable miscegenation production of hybrid seeds and seed production methods |
| CN111543312A (en) * | 2020-06-09 | 2020-08-18 | 中国热带农业科学院热带作物品种资源研究所 | Breeding method of two-line sterile line of greening new-leaf etiolation hybrid rice |
| CN116144674A (en) * | 2023-03-14 | 2023-05-23 | 西南大学 | Rice albino-transgenic green leaf mutant gene TWINKLE and application thereof |
| CN116144674B (en) * | 2023-03-14 | 2024-05-07 | 西南大学 | Rice albino transgenic green leaf mutant gene TWINKLE and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1164164C (en) | 2004-09-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2018407905B2 (en) | Photoperiod-sensitive genic male sterility mutant of upland cotton and use thereof | |
| CN107041299A (en) | A kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity | |
| CN1164164C (en) | Selective breeding process of hybrid rice with greening leaf color label carried in its seedling stage | |
| CN103004583B (en) | Rapid purification method for cucumber female flower lines | |
| Allan | Wheat | |
| DE69727233T3 (en) | LEAF TREASURES IN COMPOSITAE | |
| CN110100722B (en) | Breeding method of purple-leaf white-flower cabbage type landscape rape conventional variety | |
| CN1164165C (en) | Selective breeding process of hybrid rice with greening leaf color label carried in its seedling stage | |
| CN111436370A (en) | Potato breeding method | |
| US6018101A (en) | Method using male sterility and a marker to produce hybrid seeds and plants | |
| Miles | Evaluation of potential genetic marker traits and estimation of outcrossing rate in Stylosanthes guianensis | |
| CN105613257B (en) | A kind of samsara selection of cotton sterile line | |
| Jalikop | Rosetted siblings in F 2 of a cross in pomegranate (Punica granatum L.) can be useful model for rosetting investigations | |
| CN110073971B (en) | Breeding method of purple-leaf white-flower cabbage type rape cytoplasmic male sterility restoring line | |
| CN1692711A (en) | Method for cross breeding of corn | |
| CN112470919A (en) | Method for breeding new transgenic insect-resistant and glyphosate-resistant cotton strain | |
| AU4686996A (en) | Alfalfa products and method for producing alfalfa products for a sequential harvesting system | |
| CN1065712C (en) | Hybrid vigor fixing breeding process for rice apomixis | |
| CN115777519B (en) | Hybrid soybean mixed sowing seed production method | |
| US5457274A (en) | Pink-flowered, high protein soybean plants and methods for their production | |
| Gang-zhu et al. | Studies on bamboo hybridization | |
| CN111955340B (en) | Germplasm with inhibiting effect on blue dwarf male-sterile/blue male-sterile Ms2 trait expression and breeding method thereof | |
| CN1586156A (en) | Breed selecting method for corn with leaf color mark | |
| CN1416692A (en) | Purity identifying method for hybrid rice seed | |
| Ogden et al. | A single dominant gene, Ef, confers early flowering in acorn squash (Cucurbita pepo subsp. ovifera) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING JINSE NONGHUA SEED INDUSTRY TECHNOLOGY CO Free format text: FORMER OWNER: ZHEJIANG UNIVERSITY Effective date: 20070928 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20070928 Address after: 100080, No. 27, Zhongguancun Avenue, Haidian District, Beijing, 14 floor building Patentee after: Beijing Jinse Nonghua Seed Science & Technology Co., Ltd. Address before: Hangzhou City, Zhejiang province 310027 Xihu District Ancient Jade Road 20 Patentee before: Zhejiang University |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20040901 Termination date: 20131021 |