CN1401763A - Zinc sulfide ore leaching bacteria breeding method - Google Patents
Zinc sulfide ore leaching bacteria breeding method Download PDFInfo
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- CN1401763A CN1401763A CN02114390A CN02114390A CN1401763A CN 1401763 A CN1401763 A CN 1401763A CN 02114390 A CN02114390 A CN 02114390A CN 02114390 A CN02114390 A CN 02114390A CN 1401763 A CN1401763 A CN 1401763A
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- leaching
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
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Abstract
A process for selectively culturing the ore-leaching bacteria of zinc sulfide ore includes dividiing the iron sulfide ores into groups by magnetic separation method according to S/Fe ratios, respectively dissoving them in non-iron 9K culture media, inoculating original bacterial liquid, and culture. The resultant ore-leaching bacteria is a bacterial mixture, and has high ore-leaching speed.
Description
[technical field] the present invention relates to a kind of method of Zinc sulfide ore leaching bacteria breeding.
[background technology] zinc hydrometallurgy is made up of master operations such as concentrate roasting, flue gas acid preparing, leaching, clean liquid, electrodeposition, foundings, the metal recovery rate height, and the quality product height is easy to realize large-scale continuousization, automatic production.But traditional Zinc hydrometallurgy process long flow path, complex procedures, as the roasting of concentrate palpus, flue gas is dealt with improperly and is caused topsoil easily, and leached mud is difficult for handling, and there is the shortcoming that energy consumption is higher, pollution is bigger in the purification of leaching liquor difficulty.
Bacterium leaching-extraction-electrodeposition process has been successfully applied to the industry of metals such as uranium, gold, copper and has extracted, and it selects and great potential is arranged aspect oxidized ore, many metals mineral intergrowth and the useless assorted metal recovery handling low grade ore, difficulty.The advantage that the zinc sulfide ore bacterium is leached is that technology is simple, and with low cost, selectivity is good, handle low-grade zink sulphide or collective concentrate and have greater advantage, but the disadvantage that bacterium is leached is that the leaching kinetic rate is slow.
[summary of the invention] is in order to solve the bacterium leaching velocity difficult problem that zinc sulfide ore particularly is the ore of essential mineral with the marmatite, special proposition the present invention.
The selection, the bacterium that the present invention includes the substratum substrate are cultivated.
The bacterium that is born in the metal sulfide bed that dwells is nutrition source etch mineral with mineral, is accompanied by Fe in this process
2+Oxidation with S.With certain suitable mineral is that the substratum substrate is cultivated bacterium, according to the natural selection rule, selects bacterial classification by the mineral self-characteristic, can reduce the loss of effective bacterial classification to greatest extent, and development is fit to the dominant bacteria that mineral leach.
The present invention adopt magnetic selection method with pyrites by S/Fe ratio grouping, ore grain size is 70～90 orders, and the ore composition of different S/Fe ratios is not dissolved in the iron-free 9K substratum, inoculating original bacterial liquid is placed in the gas bath constant temperature oscillator and cultivates, 25～35 ℃ of temperature.Regulate pH value to 1.50～2.00, when bacterial growth shifts wherein 1/4 nutrient solution subculture on the fresh pyrites extremely during to logarithmic phase.It is the filtering with microporous membrane leaching supernatant liquor of 0.22 μ m that bacterium shift to be cultivated after three times with the aperture, with the bacterial suspension on the deionized water wash filter membrane of pH=1.8 to make Fe as far as possible
3+Filter, with the 9K substratum bacterium liquid is diluted at last and contains 10
8Cell/ml promptly gets as soaking the inoculated bacteria in ore deposit.
Adopt the zinc sulfide ore leaching bacterium of seed selection of the present invention that following characteristics are arranged:
(1) bacterium of seed selection is a mixed bacterium, and main bacteria seed is thiobacillus ferrooxidant and thiobacillus thiooxidans, and iron protoxide Ferrobacillus and iron protoxide leptospirillum are arranged in addition, and the ore-leaching speed of bacterium is improved significantly.
(2) characteristic of bacterium changes with the sulphur-iron ratio of substratum.In high ferro low-sulfur culture environment, thiobacillus ferrooxidant is a dominant bacteria; On the contrary, thiobacillus thiooxidans is a dominant bacteria.
(3) have stronger bacterium ferrous or the sulphur oxidation capacity and be suitable for leaching zinc ore concentrate; Mixt bacteria with balanced advantage is suitable for leaching the zinc sulphide raw ore.The effect of bacterium is that reaction product Fe is leached in oxidation
2+And element S.
[embodiment]
1.
A 〉. the selection of substratum substrate
Pyrites can be divided into different components by S/Fe ratio with magnetic selection method.Table 1 is a pyrites of taking from big factories and miness district, Guangxi, ore grain size 80 orders, and 5 groups of sample ores that sub-elect as seen from Table 1 have the S/Fe ratio of bigger variation.
Table 1: substratum substrate component
Element Weight%
No. S Fe Zn
1 30.64 67.61 1.75
2 32.34 62.01 4.65
3 28.98 58.92 5.34
4 28.59 45.75 14.09
5 30.07 35.91 7.50
B 〉. the seed selection of Zinc sulfide ore leaching bacteria
Take by weighing 15g1 in the table 1～5 respectively
#Sample ore adds 150ml iron-free 9K substratum in the 250ml Erlenmeyer flask, the original bacterium liquid of the plumbous zinc ore in big factories and miness district, area, Guangxi Hechi is taken from inoculation, is placed in the gas bath constant temperature oscillator and cultivates, 30 ℃ of temperature.Regulate pH value to 1.80, when bacterial growth shifts wherein 1/4 nutrient solution subculture on the fresh pyrites extremely during to logarithmic phase.Filter with millipore filtration (aperture 0.22 μ m) after bacterium shift to be cultivated three times and leach supernatant liquor, with the bacterial suspension on the deionized water wash filter membrane of pH=1.8 to make Fe as far as possible
3+Filter, with the 9K substratum bacterium liquid is diluted at last and contains 10
8Cell/ml prepares as the inoculated bacteria that soaks the ore deposit test.
Examine under a microscope bacterium and cultivate observation bacterial growth characteristic with the solid medium form, found that the bacterium that obtains with the pyrites seed selection is a mixed strains, wherein main bacterial classification is thiobacillus ferrooxidant and thiobacillus thiooxidans, and iron protoxide Ferrobacillus and iron protoxide leptospirillum are arranged in addition.1
#In the bacterium, thiobacillus ferrooxidant accounts for dominant advantage, 3
#, 4
#Thiobacillus thiooxidans accounts for dominant advantage in the bacterium, and 2
#With 5
#Two kinds of bacteriums quantitatively relatively more balanced.By the advantage of bacterial classification quantity as can be known the seed selection bacterium oxidation capacity of ferrous or element S is also had the branch of power.1
#The dominant bacterium of thiobacillus ferrooxidant Oxidation of Fe in the 9K substratum
2+Speed fast, on the contrary, 3
#, 4
#The dominant bacterial oxidation Fe of thiobacillus thiooxidans
2+Speed slow, and the speed of oxidation S is very fast.Pyritic sulphur-iron ratio changes as can be known in the synopsis 1, and the ferrous thiobacillus of cultivating in the doctor negative pyrites of high ferro of bacterial oxidation is preponderated, and thiobacillus thiooxidans is preponderated in the environment of the low iron of high-sulfur.
With 2
#With 5
#Bacterium is leached the mixed zinc sulfide ore of Guangxi Da Chang, contains zinc 22.24%, iron 12.8%, sulphur 23%, silicon 23.7%, ore grain size＞10mm leached 16 days, the leaching yield of zinc can reach more than 96%, and the leaching yield of impurity such as iron and silicon is all lower, and the leach liquor composition is as shown in table 2.
Table 2: leach liquor composition
Element Zn Fe SiO
2CaO MgO
Content g/L 10～28 0.4 3.0 1.20 0.63
Element Cu Cd Ni Sb As
Content mg/L 84.48 0.886 0.028 0.20 3.04 leaching effects are better than general acidleach and conventional bacterium is soaked the zinc index.
Claims (1)
1. Zinc sulfide ore leaching bacteria breeding method, it is characterized in that: the present invention adopts magnetic selection method that pyrites is divided into groups by S/Fe ratio, ore grain size is 70～90 orders, the ore composition of different S/Fe ratios is not dissolved in the iron-free 9K substratum, inoculating original bacterial liquid, be placed in the gas bath constant temperature oscillator and cultivate, 25～35 ℃ of temperature.Regulate pH value to 1.50～2.00, when bacterial growth shifts wherein 1/4 nutrient solution subculture on the fresh pyrites extremely during to logarithmic phase.It is the filtering with microporous membrane leaching supernatant liquor of 0.22 μ m that bacterium shift to be cultivated after three times with the aperture, with the bacterial suspension on the deionized water wash filter membrane of pH=1.8 to make Fe as far as possible
3+Filter, with the 9K substratum bacterium liquid is diluted at last and contains 10
8Cell/ml promptly gets as soaking the inoculated bacteria in ore deposit.
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|---|---|---|---|
| CNB021143900A CN100334197C (en) | 2002-09-10 | 2002-09-10 | Zinc sulfide ore leaching bacteria breeding method |
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| CNB021143900A CN100334197C (en) | 2002-09-10 | 2002-09-10 | Zinc sulfide ore leaching bacteria breeding method |
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| CN1401763A true CN1401763A (en) | 2003-03-12 |
| CN100334197C CN100334197C (en) | 2007-08-29 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321294A (en) * | 2020-03-05 | 2020-06-23 | 中国地质大学（武汉） | Method for improving leaching efficiency of chalcopyrite by directionally regulating and controlling indigenous microbial community |
| CN113122584A (en) * | 2021-04-22 | 2021-07-16 | 广东工业大学 | Method for extracting extracellular polymer in process of leaching fine lead-zinc sulfide ore by moderate thermophilic flora |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1350058A (en) * | 2001-12-06 | 2002-05-22 | 中南大学 | Method of breeding strain for soaking ore |
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2002
- 2002-09-10 CN CNB021143900A patent/CN100334197C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321294A (en) * | 2020-03-05 | 2020-06-23 | 中国地质大学（武汉） | Method for improving leaching efficiency of chalcopyrite by directionally regulating and controlling indigenous microbial community |
| CN111321294B (en) * | 2020-03-05 | 2021-07-09 | 中国地质大学（武汉） | Method for improving leaching efficiency of chalcopyrite by directionally regulating and controlling indigenous microbial community |
| CN113122584A (en) * | 2021-04-22 | 2021-07-16 | 广东工业大学 | Method for extracting extracellular polymer in process of leaching fine lead-zinc sulfide ore by moderate thermophilic flora |
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| CN100334197C (en) | 2007-08-29 |
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Granted publication date: 20070829 Termination date: 20100910 |