CN1056646C - Microbe and its application - Google Patents
Microbe and its application Download PDFInfo
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- CN1056646C CN1056646C CN97105354A CN97105354A CN1056646C CN 1056646 C CN1056646 C CN 1056646C CN 97105354 A CN97105354 A CN 97105354A CN 97105354 A CN97105354 A CN 97105354A CN 1056646 C CN1056646 C CN 1056646C
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- leaching
- thiobacillus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
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Abstract
The present invention relates to a microbe and an application thereof, which is characterized in that the microbe is thiobacillus ferric oxide (Thioba-cillus spp.), and the preserving registration number of the Thioba-cillus spp. Is CGMCC No. 0300. The bacterium can oxygenize elemental sulfur into sulfuric acid, and a leaching liquid is clear and has no suspended substance. Moreover, the bacterium has the strong capability of etching ore, and the bacterial leaching sulfide ore can be used for efficiently controlling a preferable condition required by bacterial leaching, quickening leaching speed and enhancing a metal recovery rate. The bacterial melt leaching technology can be used for directly producing electrolytic copper so as to have preferable economical benefits and social benefits.
Description
The present invention relates to microbial technology field, specifically a kind of microorganism and application thereof.
In the past few decades, utilize biotechnology to handle low grade ore (these ores are not suitable for traditional technology and handle) in the world and obtain remarkable progress.The impellent of these progress on the one hand is to come from market the demand of metallic substance is constantly increased, and exploitation disperses, low-grade, unmanageable ore to force people to have to, and develops new technology simultaneously and handles these ores with comparatively economic means; On the other hand, environmental pollution is serious day by day, forces people to have to seek the new technology that adapts with the social development environmental requirement, and these objective pressure make that the utilization of biotechnology in mineral engineering is more and more urgent.
At present, the application of biotechnology in mineral engineering is also not general, and its state of the art is compared with other field with range of application and still had big gap.Particularly aspect non-ferrous metals industry, all there is not the application of biotechnology so far.
The purpose of this invention is to provide a kind of by being used for the microorganism and the application thereof of the leaching of sulphide ores after the artificial culture domestication mutagenesis.
The present invention is achieved in that thiobacillus ferrooxidant (Thiobacillus spp.), and its preservation registration number is CGMCC No.0300.
The bacterial classification of field acquisition is cultivated domestication mutagenesis under the following conditions get thiobacillus ferrooxidant ((Thiobacillus spp.), this bacterial strain is in the preservation of specified depositary institution of Patent Office of the People's Republic of China, preservation date is on May 6th, 1997, and its preservation registration number is CGMCC No.0300.Ne ar is characterized as: thiobacillus ferrooxidant, shaft-like: 0.5 * 10-1.7 micron, 1.0 * 1,7 microns, typical gram-negative bacteria, single flagellum, movable; Spherical: the 0.8-1.0 micron, typical gram positive organism, not movable.They have active good, and leaching velocity is fast, characteristics such as easy cultivation.Thalline itself there is no direct harm to humans and animals.They can with contain that sulfur mineral is sent out and thing such as uranium ore phosphorus ore in sulphur be oxidized to sulfuric acid, sulfuric acid is further metallic sulfides such as the copper in the oxide mineral, lead, zinc again, make free, the dissolving of metal in the mineral, just can obtain elemental metals by replacement(metathesis)reaction again.
Culture condition:
1. the composition of substratum (g/L), following each content can float 1 times or float downward 10 times:
Ferrous sulfate 40
Sal epsom 0.5
Ammonium sulfate 3
Repone K 0.5
Dipotassium hydrogen phosphate 0.5
Nitrocalcite 0.01
2. culture condition: temperature 20-30 ℃, pH value 1.5-2.5, (CO2 O2), adds substratum, adds the thiobacillus ferrooxidant bacterial classification to blast air;
3. carrier is that water adds the liquid 1L that dilute sulphuric acid is adjusted to PH=1.5-2.5.
The bacterium that above-mentioned gained microorganism-thiobacillus ferrooxidant (Thiobacillus spp.) is used for sulphide ores is leached.
The present invention compared with prior art has following positively effect:
1, the present invention cultivates the bacterium of taming out and can make the sulphide ores ore-leaching speed accelerate that (leaching yield reached 69.881% in 171 days, wherein oxidation ratio 27.15%), its vitality is stronger, and cultivation fee is low (when reaching 1000 tons of copper scales, microbial culture take less than 140 yuan/TCu), prospects for commercial application is wide.
2, this bacterium can be oxidized to sulfuric acid to elementary sulfur, leaches the limpid and no suspended substance matter of feed liquid.This bacterium etch ore ability is strong, becomes very loose through the ore part behind this bacterial oxidation, and mineral lattice is damaged.
3, utilize this bacterium to leach sulphide ores and can control the required top condition of bacterium leaching effectively, accelerate leaching velocity, improve metal recovery rate.And broken must possess high-sulfur, high iron material can adopt bacterium to leach the viewpoint of sulphide ores.
4, adopt this technology can the direct production electrolytic copper, have favorable economic benefit and social benefit.
5, handle the higher low-grade sulfide ore of sulphur content.The ore that copper content is lower utilizes traditional technology to give birth to, and is infeasible economically.Adopt this technology, because sulphur content is higher, produce sulfuric acid when bacterium is leached, the acid consumption is reduced, resource is rationally utilized.
6, created good condition for the sulphide ores that reclaims in the in-situ leaching ore.Also for reclaiming the sulphide ores under other condition, the direct production electrolytic copper provides a new way.
7, this bacterium extract technology is free from side effects to extraction-electrodeposition, and bacterium is to the person poultry harmless, and is free from environmental pollution, has footpath after the bigger development.
Fig. 1 is a bacterial classification aspect graph of the present invention
Embodiment obtains bacterial classification from the field, and it is cultivated under following substratum and condition:
1. the composition of substratum (g/L)
Ferrous sulfate 40
Sal epsom 0.5
Ammonium sulfate 3
Repone K 0.5
Dipotassium hydrogen phosphate 0.5
Nitrocalcite 0.01
2. culture condition: 30 ℃ of temperature, pH value 2, (CO2 O2), adds substratum, adds the bacterial classification thiobacillus ferrooxidant to blast air;
3. the carrier that uses is liquid, and promptly water adds dilute sulphuric acid and is adjusted to PH=1.5-2.5, and used liquid is 1 liter.
The thiobacillus ferrooxidant (Thiobacillus spp.) of gained is used for the bacterium leaching of sulphide ores.The copper-sulphide ores that is about to after the fragmentation enters the heap field boundary, with the sulphuric acid soln that contains bacterium ore is sprayed, and carries out that bacterium is molten to be soaked, and after the cupric liquid collecting after molten the soaking, the clarification, utilizes the copper extractant extraction, back extraction, after the enrichment, behind electrodeposition process electrolytic copper.
Claims (3)
1, a kind of microorganism is characterized in that it is thiobacillus ferrooxidant (Thiobacillus spp.), and its preservation registration number is CGMCC No.0300.
2, by the described microorganism of claim 1, it is characterized in that thiobacillus ferrooxidant is cultivated under the following conditions:
1. the composition of substratum (g/L), following each content can float 1 times or float downward 10 times
Ferrous sulfate 40
Sal epsom 0.5
Ammonium sulfate 3
Repone K 0.5
Dipotassium hydrogen phosphate 0.5
Nitrocalcite 0.01
2. culture condition: temperature 20-30 ℃, pH value 1.5-2.5, (CO2 O2), adds substratum, adds the thiobacillus ferrooxidant bacterial classification to blast air;
3. carrier is that water adds the liquid 1L that dilute sulphuric acid is adjusted to PH=1.5-2.5.
3, a kind of application of microorganism is characterized in that thiobacillus ferrooxidant (Thiobacillus spp.) is used for the bacterium leaching of sulphide ores.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97105354A CN1056646C (en) | 1997-07-04 | 1997-07-04 | Microbe and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97105354A CN1056646C (en) | 1997-07-04 | 1997-07-04 | Microbe and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1204691A CN1204691A (en) | 1999-01-13 |
| CN1056646C true CN1056646C (en) | 2000-09-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97105354A Expired - Fee Related CN1056646C (en) | 1997-07-04 | 1997-07-04 | Microbe and its application |
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| CN (1) | CN1056646C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100368531C (en) * | 2005-12-05 | 2008-02-13 | 东华理工学院 | Acidophilic leptospirillumferrooxidans and its uses |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PE20060789A1 (en) | 2004-10-22 | 2006-08-10 | Biosigma Sa | WENELEN BACTERIA STRAIN DSM 16786 AND LEACHING PROCESS BASED ON INOCULATION OF SAID STRAIN |
| CN102826611A (en) * | 2012-09-13 | 2012-12-19 | 中国科学院成都生物研究所 | Microbiology method for improving aqueous dispersibility of ferric oxide ultrafine powder body |
| CN103848459B (en) * | 2014-01-29 | 2015-08-26 | 浙江工业大学 | A kind of biomineralization legal system is for sea urchin shape α-Fe 2o 3method |
| CN104531992B (en) * | 2014-12-23 | 2017-02-22 | 中南大学 | Application of ferric phosphate for reinforcement of bacterial leaching out of nickel sulphide ores |
| CN108034619A (en) * | 2018-01-26 | 2018-05-15 | 南华大学 | A kind of preparation method of galvanotaxis microorganism formulation and its application in uranium-containing waste water is handled |
| CN109487093B (en) * | 2018-10-26 | 2021-08-17 | 北票市金海矿业有限公司 | Hydrometallurgical leaching treatment |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107518A (en) * | 1994-07-08 | 1995-08-30 | 南京林业大学 | Method for microbial-chemical dephosphorization of iron ore |
| CN1118378A (en) * | 1994-09-06 | 1996-03-13 | 吉林省冶金研究所 | Pre-oxidating method for difficultly beneficiated gold containing ore or concentrated mineral by bacteria |
-
1997
- 1997-07-04 CN CN97105354A patent/CN1056646C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107518A (en) * | 1994-07-08 | 1995-08-30 | 南京林业大学 | Method for microbial-chemical dephosphorization of iron ore |
| CN1118378A (en) * | 1994-09-06 | 1996-03-13 | 吉林省冶金研究所 | Pre-oxidating method for difficultly beneficiated gold containing ore or concentrated mineral by bacteria |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100368531C (en) * | 2005-12-05 | 2008-02-13 | 东华理工学院 | Acidophilic leptospirillumferrooxidans and its uses |
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| Publication number | Publication date |
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| CN1204691A (en) | 1999-01-13 |
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