CN100334197C - Zinc sulfide ore leaching bacteria breeding method - Google Patents
Zinc sulfide ore leaching bacteria breeding method Download PDFInfo
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- CN100334197C CN100334197C CNB021143900A CN02114390A CN100334197C CN 100334197 C CN100334197 C CN 100334197C CN B021143900 A CNB021143900 A CN B021143900A CN 02114390 A CN02114390 A CN 02114390A CN 100334197 C CN100334197 C CN 100334197C
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- bacterium
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- zinc sulfide
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
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Abstract
The present invention relates to a selection method of a leaching bacterium of zinc sulfate ore. In the method, a magnetic selection method is adopted to group iron sulfide ore according to a ratio of S to Fe, the ore groups with different ratios of S to Fe are respectively dissolved in a 9K nonferrous culture medium, the culture medium is inoculated with original bacterium liquid, and the original bacterium liquid is cultured to obtain the leaching bacterium. The zinc sulfate ore leaching bacterium selected by the method is a mixed bacterium, the main bacterial strains are thiobacillus ferrooxidans and thiobacillus thiooxidans, ferrobacillus ferrooxidans and leptospirillum ferrooxidans are contained in the mixed bacterium, and the leaching speed of the bacterium is obviously increased; the characteristics of the bacterium are changed according to change of the ratio of S to Fe in the culture medium; the bacterium with a strong capability for oxidizing ferrous iron or sulfur is suitable for leaching zinc concentrate ore; the mixed bacterium with a balanced advantage is suitable for leaching crude zinc sulfate ore.
Description
[technical field] the present invention relates to a kind of method of Zinc sulfide ore leaching bacteria breeding.
[background technology] zinc hydrometallurgy is made up of master operations such as concentrate roasting, flue gas acid preparing, leaching, clean liquid, electrodeposition, foundings, the metal recovery rate height, and the quality product height is easy to realize large-scale continuousization, automatic production.But traditional Zinc hydrometallurgy process long flow path, complex procedures, as the roasting of concentrate palpus, flue gas is dealt with improperly and is caused topsoil easily, and leached mud is difficult for handling, and there is the shortcoming that energy consumption is higher, pollution is bigger in the purification of leaching liquor difficulty.
Bacterium leaching-extraction-electrodeposition process has been successfully applied to the industry of metals such as uranium, gold, copper and has extracted, and it selects and great potential is arranged aspect oxidized ore, many metals mineral intergrowth and the useless assorted metal recovery handling low grade ore, difficulty.The advantage that the zinc sulfide ore bacterium is leached is that technology is simple, and with low cost, selectivity is good, handle low-grade zink sulphide or collective concentrate and have greater advantage, but the disadvantage that bacterium is leached is that the leaching kinetic rate is slow.
[summary of the invention] is in order to solve the bacterium leaching velocity difficult problem that zinc sulfide ore particularly is the ore of essential mineral with the marmatite, special proposition the present invention.
The selection, the bacterium that the present invention includes the substratum substrate are cultivated.
The bacterium that is born in the metal sulfide bed that dwells is nutrition source etch mineral with mineral, is accompanied by Fe in this process
2+Oxidation with S.With certain suitable mineral is that the substratum substrate is cultivated bacterium, according to the natural selection rule, selects bacterial classification by the mineral self-characteristic, can reduce the loss of effective bacterial classification to greatest extent, and development is fit to the dominant bacteria that mineral leach.
The present invention adopt magnetic selection method with pyrites by S/Fe ratio grouping, ore grain size is 70~90 orders, and the ore composition of different S/Fe ratios is not dissolved in the iron-free 9K substratum, inoculating original bacterial liquid is placed in the gas bath constant temperature oscillator and cultivates, 25~35 ℃ of temperature.Regulate pH value to 1.50~2.00, when bacterial growth shifts wherein 1/4 nutrient solution subculture on the fresh pyrites extremely during to logarithmic phase.It is the filtering with microporous membrane leaching supernatant liquor of 0.22 μ m that bacterium shift to be cultivated after three times with the aperture, with the bacterial suspension on the deionized water wash filter membrane of pH=1.8 to make Fe as far as possible
3+Filter, with the 9K substratum bacterium liquid is diluted at last and contains 10
8Cell/ml promptly gets as soaking the inoculated bacteria in ore deposit.
Adopt the zinc sulfide ore leaching bacterium of seed selection of the present invention that following characteristics are arranged:
(1) bacterium of seed selection is a mixed bacterium, and main bacteria seed is thiobacillus ferrooxidant and thiobacillus thiooxidans, and iron protoxide Ferrobacillus and iron protoxide leptospirillum are arranged in addition, and the ore-leaching speed of bacterium is improved significantly.
(2) characteristic of bacterium changes with the sulphur-iron ratio of substratum.In high ferro low-sulfur culture environment, thiobacillus ferrooxidant is a dominant bacteria; On the contrary, thiobacillus thiooxidans is a dominant bacteria.
(3) have stronger bacterium ferrous or the sulphur oxidation capacity and be suitable for leaching zinc ore concentrate; Mixt bacteria with balanced advantage is suitable for leaching the zinc sulphide raw ore.The effect of bacterium is that reaction product Fe is leached in oxidation
2+And element S.
[embodiment]
1.
A 〉. the selection of substratum substrate
Pyrites can be divided into different components by S/Fe ratio with magnetic selection method.Table 1 is a pyrites of taking from big factories and miness district, Guangxi, ore grain size 80 orders, and 5 groups of sample ores that sub-elect as seen from Table 1 have the S/Fe ratio of bigger variation.
Table 1: substratum substrate component
No. | Element Weight% | ||
S | Fe | Zn | |
1 2 3 4 5 | 30.64 32.34 28.98 28.59 30.07 | 67.61 62.01 58.92 45.75 35.91 | 1.75 4.65 5.34 14.09 7.50 |
B 〉. the seed selection of Zinc sulfide ore leaching bacteria
Take by weighing 15gl in the table 1~5 respectively
#Sample ore adds 150ml iron-free 9K substratum in the 250ml Erlenmeyer flask, the original bacterium liquid of the plumbous zinc ore in big factories and miness district, area, Guangxi Hechi is taken from inoculation, is placed in the gas bath constant temperature oscillator and cultivates, 30 ℃ of temperature.Regulate pH value to 1.80, when bacterial growth shifts wherein 1/4 nutrient solution subculture on the fresh pyrites extremely during to logarithmic phase.Filter with millipore filtration (aperture 0.22 μ m) after bacterium shift to be cultivated three times and leach supernatant liquor, with the bacterial suspension on the deionized water wash filter membrane of pH=1.8 to make Fe as far as possible
3+Filter, with the 9K substratum bacterium liquid is diluted at last and contains 10
8Cell/ml prepares as the inoculated bacteria that soaks the ore deposit test.
Examine under a microscope bacterium and cultivate observation bacterial growth characteristic with the solid medium form, found that the bacterium that obtains with the pyrites seed selection is a mixed strains, wherein main bacterial classification is thiobacillus ferrooxidant and thiobacillus thiooxidans, and iron protoxide Ferrobacillus and iron protoxide leptospirillum are arranged in addition.1
#In the bacterium, thiobacillus ferrooxidant accounts for dominant advantage, 3
#, 4
#Thiobacillus thiooxidans accounts for dominant advantage in the bacterium, and 2
#With 5
#Two kinds of bacteriums quantitatively relatively more balanced.By the advantage of bacterial classification quantity as can be known the seed selection bacterium oxidation capacity of ferrous or element S is also had the branch of power.1
#The dominant bacterium of thiobacillus ferrooxidant Oxidation of Fe in the 9K substratum
2+Speed fast, on the contrary, 3
#, 4
#The dominant bacterial oxidation Fe of thiobacillus thiooxidans
2+Speed slow, and the speed of oxidation S is very fast.Pyritic sulphur-iron ratio changes as can be known in the synopsis 1, and the ferrous thiobacillus of cultivating in the doctor negative pyrites of high ferro of bacterial oxidation is preponderated, and thiobacillus thiooxidans is preponderated in the environment of the low iron of high-sulfur.
With 2
#With 5
#Bacterium is leached the mixed zinc sulfide ore of Guangxi Da Chang, contains zinc 22.24%, iron 12.8%, sulphur 23%, silicon 23.7%, ore grain size>10mm leached 16 days, the leaching yield of zinc can reach more than 96%, and the leaching yield of impurity such as iron and silicon is all lower, and the leach liquor composition is as shown in table 2.
Table 2: leach liquor composition
Constituent content g/L | Zn 10~28 | Fe 0.4 | SiO 2 3.0 | CaO 1.20 | MgO 0.63 |
Constituent content mg/L | Cu 84.48 | Cd 0.886 | Ni 0.028 | Sb 0.20 | As 3.04 |
Leaching effect is better than general acidleach and conventional bacterium is soaked the zinc index.
Claims (1)
1. Zinc sulfide ore leaching bacteria breeding method, it is characterized in that: adopt magnetic selection method that pyrites is pressed the grouping of S/Fe ratio, ore grain size is 70~90 orders, the ore composition of different S/Fe ratios is not dissolved in the iron-free 9K substratum, inoculating original bacterial liquid, be placed in the gas bath constant temperature oscillator and cultivate, 25~35 ℃ of temperature, regulate pH value to 1.50~2.00, when bacterial growth shifts wherein 1/4 nutrient solution subculture to the fresh pyrites during to logarithmic phase, it is the filtering with microporous membrane leaching supernatant liquor of 0.22 μ m that bacterium shift to be cultivated after three times with the aperture, with the bacterial suspension on the deionized water wash filter membrane of pH=1.8 to make Fe as far as possible
3+Filter, with the 9K substratum bacterium liquid is diluted at last and contains 10
8Cell/ml promptly gets as soaking the inoculated bacteria in ore deposit.
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CN100334197C true CN100334197C (en) | 2007-08-29 |
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CN111321294B (en) * | 2020-03-05 | 2021-07-09 | 中国地质大学(武汉) | Method for improving leaching efficiency of chalcopyrite by directionally regulating and controlling indigenous microbial community |
CN113122584A (en) * | 2021-04-22 | 2021-07-16 | 广东工业大学 | Method for extracting extracellular polymer in process of leaching fine lead-zinc sulfide ore by moderate thermophilic flora |
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CN1350058A (en) * | 2001-12-06 | 2002-05-22 | 中南大学 | Method of breeding strain for soaking ore |
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CN1350058A (en) * | 2001-12-06 | 2002-05-22 | 中南大学 | Method of breeding strain for soaking ore |
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Granted publication date: 20070829 Termination date: 20100910 |