CN101899570B - Preoxidation treating method for leaching arsenic golden ores by using drum type reactor organisms - Google Patents
Preoxidation treating method for leaching arsenic golden ores by using drum type reactor organisms Download PDFInfo
- Publication number
- CN101899570B CN101899570B CN2010102395892A CN201010239589A CN101899570B CN 101899570 B CN101899570 B CN 101899570B CN 2010102395892 A CN2010102395892 A CN 2010102395892A CN 201010239589 A CN201010239589 A CN 201010239589A CN 101899570 B CN101899570 B CN 101899570B
- Authority
- CN
- China
- Prior art keywords
- arsenic
- gas
- ore
- mutagenesis
- gold ore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
Landscapes
- Manufacture And Refinement Of Metals (AREA)
Abstract
The invention relates to a preoxidation treating method for leaching arsenic golden ores by using drum type reactor organisms. The method comprises the following steps of: (1) rinsing arsenic golden concentrate ores; (2) filtering, drying, grinding, activating and acid presoaking the rinsed arsenic golden concentrate ores; (3) acclimating and culturing the induced bacteria of acidithiobacillus ferrooxidans and acidithiobacillus thiooxidans, and amplifying and culturing to prepare induced mixing bacteria; (4) inoculating the induced mixed bacteria into the drum type reactor to leach the organisms of high-concentration arsenic golden ores; and (5) cyanizing and extracting golden from the arsenic golden ores leached residues. The drum type reactor comprises a gas distributor, wherein the gas distributor comprises a gas inlet pipe, a gas guide branch pipe, a gas distributing pipe and a gas outlet pipe. The gas distributor realizes the gas predispersing and sufficient gas and liquid contact and mixing, and promotes the sufficient gas and liquid mass transfer and dissolved oxygen supply. The invention fundamentally solves the problems of high mass transfer rate and low shearing in high ore slurry concentration, and realizes the high-efficiency biology preoxidation of arsenic golden ores.
Description
Technical field
The present invention relates to the bio-oxidation treatment process of arsenic-bearing gold ore; Particularly; The present invention relates to a kind of biological pre-oxidation treatment method that leaches the high density arsenic-containing gold ore of rotating drum type reactor that adopts, use the mutagenesis mixed bacterium to leach the pre-oxidation treatment method of arsenic-containing gold ore at rotating drum type reactor.
Background technology
Arsenic-bearing gold ore is a kind of typical difficult-treating gold mine; Adopt traditional cyanide process directly to handle, the leaching yield of gold is very low, and its treatment process is different with conventional gold mine; Need before carrying out cyaniding, increase a pretreatment process; Its objective is and to carry gold mineral such as pyrite, arsenopyrite and the decomposition of other metallic sulfide oxidations, and, gold is come out from sulfide inclusions oxidized sulphur, arsenic etc. and ore separation; Remove the element that hinders cyaniding simultaneously, make conventional cyanide process soak gold and can obtain suitable ideal index.
At present the pretreatment technology of arsenic-bearing gold ore mainly contains roasting, hot pressing and bio-oxidation etc., the technological comparative maturity of roasting and hot-pressure oxidation wherein, but have problems such as gold recovery is low, environmental pollution is serious.Microbial oxidation pre-treatment indissoluble gold ore deposit is to utilize the golden mineral of bacterial oxidation parcel that gold is exposed to be easy to cyaniding.Its major advantage one is an environmentally safe, has not both had the smoke gas treatment requirement of roasting method, does not have the high pressure potentially dangerous of pressure oxidation again; The 2nd, flow process is simple, less investment, low, the less energy consumption of cost, requires low to operant level; The 3rd, it is more reasonable to handle the production and operation of higher-grade Gold Ore on a small scale, and method is compared therewith, and pressure oxidation is more worthwhile economically to large-scale plant (more than the 1200t/d).
Microbial oxidation is used for pre-treatment indissoluble gold ore deposit, is the important breakthrough of biotechnology on the mining industry Application and Development, from the seventies in 20th century Application and Development so far, this method just progressively moves to maturity.Domestic after building up a 10t/d pilot plant aspire to Xi'an 20 end of the centurys, again in December, 2000 build up in the smeltery, Yantai domestic first hand have independent intellectual property right the 50t/d scale carry golden demonstration plant; Smeltery, Laizhou, Shandong and Australia cooperate with South Africa, and a complete set of introduction of foreign technology and equipment have been built the 200t/d production plant; Fengcheng City, Liaoning utilizes Changchun Gold Research Institute's technology to establish the 100t/d gold extracting shop.Because this method less investment; Production cost is low, and advantages such as non-environmental-pollution have become noticeable research field in the world wide; Accepted by people just day by day; And being successfully applied to industrial production, the important effect of play more and more in the pre-treatment of refractory gold ore has become domestic and international refractory gold ore at present and has selected one of important means of smelting.
Bio-oxidation is handled the indissoluble gold ore deposit and is mainly adopted tank leaching process.Traditional stirring-type/air lift type bioleaching reactor drum is by stirring the driving liquid-flow, drive particle suspension by liquid-flow, the bigger power of agitator of raising requirement of pellet density or concentration; The 2nd, the raising of pulp density makes ore pulp retrogradation, viscosity increase, and is unfavorable for gas dispersion, and it is long-pending promptly to need bigger power of agitator could improve liquid-gas interface, and causes the increase of shearing force like this.After pulp density surpasses 20% (w/v), for making the mineral grain mixing that fully suspends, have to import bigger power of agitator, bigger shearing force damages thalline.The shortcoming that present this technology also exists is that requirement pulp density, potential of hydrogen and temperature control condition etc. are harsh; Oxidation rate is slow, the flow process time is long and oxidation effectiveness is undesirable etc.
The root problem of traditional biological leaching reactor is high mass transfer and the low contradiction of shearing.Characteristics such as adopt the novel rotary drum that proposes recently biologically to leach reactor drum (CN200610144193) and have high mass transfer, lowly shear, gas distribution is even; The biological pre-oxidation that is applied to arsenic-bearing gold ore is handled; Under high pulp density, realize high mass transfer and the low contradiction of shearing with fundamentally solving, help accelerating bio-oxidation speed, the shortening leaching cycle of arsenic-containing gold ore and improve leaching efficiency.
Summary of the invention
The objective of the invention is in order to solve the high mass transfer that exists in the above-mentioned traditional biological leaching reactor and the low contradiction of shearing, thereby a kind of biological pre-oxidation treatment method that leaches arsenic-containing gold ore of rotating drum type reactor that adopts is provided.
Be realization foregoing invention purpose, a kind of biological pre-oxidation treatment method that leaches the high density arsenic-containing gold ore of rotating drum type reactor that adopts provided by the invention, this method may further comprise the steps:
1) will use the tap water rinsing through the arsenic-containing gold concentrate of flotation;
2) with carrying out the drainage oven dry after the rinsing in the step 1), grinding activation, sour preimpregnation;
3) will have a liking for ferrous thiobacillus of acid oxidase and the mutagenesis bacterium of having a liking for acid oxidase sulphur thiobacillus, carry out acclimation shaking culture;
4) the mutagenesis mixed bacterium with amplification culture in the step 3) is inoculated into the high density arsenic-containing gold ore after acidleach, in rotating drum type reactor, carries out biology and leaches;
The slag that soaks that 5) will leach that operation comes out carries out cyanidation gold-extractedly, and leach liquor is through analyzing and testing and after removing harmful ion, and circulation is used for the continuous leaching of arsenic-bearing gold ore;
Carry out the biological pre-oxidation of high density arsenic-containing gold ore in the described rotating drum type reactor; Directly promote mineral grain through the rotary drum interior panelling and carry out the ore pulp uniform mixing; Realize by gas distributor that gas is pre-dispersed and fully contact mixing with gas-liquid, promotion gas-liquid mass transfer and dissolved oxygen supply are abundant.
This rotating drum type reactor comprises a gas distributor, and said gas distributor comprises: inlet pipe 1, branch's gas tube 2, gas distribution tube 3 and escape pipe 4.
Described inlet pipe 1 wears in the rotating shaft of being fixed in rotary drum one end, and the outlet side that this inlet pipe 1 is positioned at rotary drum is communicated to some gas distribution tubes 3 through branch's gas tube 2; Described branch gas tube 2 semicirculars in shape, some gas distribution tubes 3 are along the axially parallel setting of rotary drum; Described escape pipe 4 is arranged on the horizontal fixed axle of rotary drum one end, and its end expands part for cylindrical microporous collection and confinement of gases pipe, and this microporous collection and confinement of gases is guaranteed and is held in more than the liquid level its top end surface sealing, side perforate.
A kind of improvement as technique scheme; In the step 1) arsenic-containing gold ore is carried out the repeated multiple times rinsing to clarification with tap water; Removing flotation reagent or other objectionable impuritiess that the arsenic-containing gold ore powder adds in the processing purification process, to prevent that flotation reagent etc. is to the murder by poisoning of ore immersion bacterial culture or suppress its biological activity.
As another improvement of technique scheme, step 2) in rinsing ore drainage is placed in the loft drier, temperature-stable is 110～120 ℃; The dry sample ore of crossing grinds activation, more than the continuously grinding 30min; Sample ore after grinding is carried out acidleach, and the mass ratio of sample ore and pickling liquor is 1: 2 or 1: 3, controls the pH of soak solution and is stabilized in 1.5～2.0 through adding sulphuric acid soln in the immersion process, noticeable change does not take place to pH.
As another improvement of technique scheme, the mutagenesis mixed bacterium is raised and train and may further comprise the steps in the step 3):
3-1) with the uv lamp of 15～20W, wavelength 265nm, preheating 20～30min operates in camera bellows; Have a liking for the ferrous thiobacillus of acid oxidase and the petridish of having a liking for acid oxidase sulphur thiobacillus 10～20ml, diameter 9～15cm apart from uv lamp 30～40cm with filling bacterium liquid, carry out radiotreatment by different time; Bacterium liquid lucifuge was handled 12～24 hours in 4 ℃ of refrigerators after the mutagenesis, was placed on lucifuge cultivation in the shaking table, obtained the gain mutant bacterial strain of 2 kinds of enrichments respectively;
3-2), leach the continuous domestication of carrying out the mutagenesis bacterial classification through shaking bottle respectively, base in 250～1000ml Erlenmeyer flask and Leathen cultivation, Fe with the mutagenic strain of 2 kinds of ore immersion bacterial culture enrichments
2+Or sulphur powder amount reduces and mode that arsenic-containing gold ore powder ratio increases gradually gradually, increases arsenic-containing gold ore 2%～5% at every turn, just reduces Fe
2+Or sulphur powder amount 2%～5%, when arsenic-containing gold ore content greater than 30% the time, stop to use Fe
2+Or the sulphur powder is made the energy; Control acclimation shaking culture base pH is 1.5～2.5, and to having a liking for the cultivation of going down to posterity of ferrous thiobacillus of acid oxidase and thiobacillus thiooxidans, culture temperature is 25～35 ℃ respectively; Continuous domestication is cultivated more than five generations and is obtained concentration greater than 10
7The flexibility domestication bacterium of individual/ml;
3-3) in optimizing the Leathen substratum, and add Fe in proportion
2+And elemental sulfur, mixing amplification culture through continuous round-robin mutagenesis bacterial classification; At first carry out the batch shake-flask culture less than the Gold Ore of 75 μ m, in 10L, 20L and 50L rotary drum reactor drum, carry out amplification culture step by step then at 90% above mineral granularity.Wherein inoculum size be the culture TV 10%～30%, temperature between 30～35 ℃, the pH value is between 1.5～2.5, adding the breeze amount is 20%～50% of culture gross weight, obtaining inoculum density is 10
7～10
9The mutagenesis mixed bacterium of high ore pulp of the adaptation of individual/ml and high ion concentration.
Also a kind of biological arsenic-containing gold ore that leaches high pulp density in rotating drum type reactor of the employing mutagenesis mixed bacterium described in the step 4) that improves as technique scheme may further comprise the steps:
4-1) sample ore after the acidleach is joined in the rotary drum reactor drum, add leach liquor and inoculation mutagenesis mixed bacterium in proportion.The control pulp density is 20～50%, and the bacterial classification initial density is greater than 10
7Individual/ml;
4-2) leach liquor is prepared with tap water, adds nutrition composition by the Leathen substratum of optimizing, and contains (NH in the 1L substratum
4)
2SO
41.5g, MgSO
47H
2O 0.05g, KCl 0.05g, K
2HPO
40.05g, Ca (NO
3)
20.01g;
4-3) in the arsenic-containing gold ore bioleaching process, the controlling reactor temperature is that 30～35 ℃, pH value 1.5～2.0, Ventilation Rate are that 2～20L/min, rotary drum rotating speed are 1～10r/min.
The principle of utilizing this technology to carry out the indissoluble gold ore deposit is: remove flotation reagent or other objectionable impuritiess that the arsenic-containing gold ore powder adds through rinsing in the processing purification process, to prevent that flotation reagent etc. is to the murder by poisoning of ore immersion bacterial culture or suppress its biological activity; Arsenic-containing gold ore is carried out drainage/exsiccant remove moisture in the arsenic-containing gold ore and subsequent disposal after rinsing, so that carry out the pulp density proportioning of biological leaching system; The purpose that arsenic-containing gold ore is ground is the particle diameter and the crystalline structure that changes particle surface that reduces mineral grain, helps increasing the contact area and the oxidation dissolution that promotes sulphide ores in the Gold Ore of mineral grain and leach liquor; The acid preimpregnation be with in the acid with arsenic-bearing gold ore in alkaline components, to prevent in the bioleaching process that pH takes place sharply to change, make the leaching system be stabilized in the appropriate pH scope of suitable microorganism growth; Adopting the mutagenesis associate strain is the mutagenesis mixed bacterium of having a liking for the ferrous thiobacillus of acid oxidase (At.f) and having a liking for acid oxidase sulphur thiobacillus (At.t); After acclimation shaking culture; Obtain the mutagenesis mixed bacterium of anti-high pulp density of flexibility and high ion concentration, be used for rotating drum type reactor and leach arsenic-bearing gold ore; It is through cultivating the good mutagenesis associate strain of domestication that inoculation is leached, and is 10～30% to be inoculated in the arsenic-bearing gold ore ore pulp of acidleach by inoculum size, in revolving drum type bioreactor, carries out biological pre-oxidation and handles, and leaches to accomplish the back recovery and soak slag and be used for cyanidation gold-extracted.
The invention has the advantages that, well overcome the high mass transfer that exists in the present traditional biological leaching reactor and the low contradiction of shearing, and defective such as the pulp density that is caused thus is lower, oxidation rate slow, the flow process time is long and oxidation effectiveness is undesirable.Ore deposit concentration is soaked in shearing action and the raising of adopting revolving drum type bioreactor can reduce in the bioleaching process; Can realize that high mass transfer, uniform gas-liquid distribution and dissolved oxygen supply under the high pulp density are abundant, reach and accelerate leaching rate and the target that improves leaching effect.
Description of drawings
Fig. 1 the present invention adopts the biological process flow sheet that leaches arsenic-containing gold ore of rotating drum type reactor.
The revolving drum type bioreactor structural representation that Fig. 2 the present invention adopts.
The side schematic view of Fig. 3 gas distributor of the present invention.
The axial vertical cross-section synoptic diagram of Fig. 4 gas distributor of the present invention.
Reference numeral
1. inlet pipe 2. branch's gas tubes 3. gas distribution tubes
4. escape pipe 5. fixing horizontal axles 6. fixed supports
7. baffle plate 8. water bath with thermostatic control coiled pipes 9. rotary drums
Embodiment
Below in conjunction with accompanying drawing and embodiment, the present invention is carried out further detailed explanation.
The rotary drum reactor drum is as shown in Figure 2 among the present invention, and wherein related gas distributor comprises: inlet pipe 1, branch's gas tube 2, gas distribution 3 pipe and escape pipes 4, and as shown in Figure 3.Described inlet pipe 1 wears on the horizontal fixed axle that is fixed in rotary drum 9 one ends; Single inlet pipe gets in the rotary drum, and inlet pipe one end connects branch's gas tube 2, is communicated to some gas distribution tubes 3 via branch's gas tube 2; Gas distribution tube can be a several layers; Axially be provided with along rotary drum, and be connected reinforcing on the water bath with thermostatic control coiled pipe 8 through fixed support 6, as shown in Figure 2; Curved distribution on many other distribution pipes and the axial vertical cross section, branch's gas tube semicircular in shape, as shown in Figure 4.Escape pipe 4 is L-shaped; As shown in Figure 1; And be arranged on the horizontal fixed axle 5 of rotary drum one end, its end that is positioned at reactor drum is disposed radially, and its end expands and partly is cylindrical microporous collection and confinement of gases pipe; This microporous collection and confinement of gases is guaranteed and is held in more than the liquid level, its top end surface sealing, side perforate.This rotating drum type reactor directly promotes mineral grain through rotary drum interior panelling 7 and carries out the ore pulp uniform mixing.
Simulate mineral with the aluminum oxide inert particle, change reactor drum middle particle concentration (particle collision frequency) through the alumina particle that in revolving drum type bioreactor, adds different amounts, with mutagenesis mixed bacterium Oxidation of Fe
2+The different granule density of procedural representation to the influence of leaching microbacteria oxidation activity.In the control leaching process, rotary drum bio-reactor rotating speed is 3.33r/min, and Ventilation Rate is 6L/min.Inoculum size is 3% of a liquid volume, 30 ℃ of temperature of reaction.Under various concentration of solid particles, Fe in the rotary drum bio-reactor
2+Oxidising process demonstrate identical characteristic, i.e. Fe in initial 8h
2+Concentration does not have to reduce basically, and the lag phase influence in the corresponding bacteria growth process is different.At low solids concn
Down, rotary drum bio-reactor Fe in stirred pot
2+The oxidising process curve very approaching.Be increased to 400g/L and 500g/L with solids concn, Fe in the rotary drum bio-reactor
2+Oxidising process change not obvious; And in stirred pot, Fe
2+Oxidising process obviously is extended.Be illustrated under the different concentration of solid particles Fe in rotary drum bio-reactor and the stirred pot
2+Maximum rate of oxidation basically identical.But after concentration of solid particles reaches 200g/L, improve concentration of solid particles again, can prolong the lag phase of bacterial growth in the stirred pot; In this scope, do not influence in the rotary drum bio-reactor the lag phase of bacterium is tangible.
In revolving drum type bioreactor, carry out the biological leaching experiment of arsenic-containing gold ore, the performance of examination rotary drum reactor drum in actual bioleaching process.Do not add Fe in the seed liquor
2+And adding the energy of sample ore as microorganism growth, inoculum size is 10% of a liquid volume.The same embodiment of temperature, rotating speed and Ventilation Rate (1).The objectionable impurities that discharges in the bioleaching process, like the oxidation products of arsenic, may be to the active restraining effect that produces of leaching microbacteria.For avoiding the influence of above-mentioned factor, keep mineral concentration consistent, and change total solids granule density in the reactor drum through the alumina particle that adds different concns.The bioleaching process of arsenic-containing gold ore in revolving drum type bioreactor, Zong concentration of iron with carrying out of leaching of biology and raise gradually, its total solid concentration is that total concentration of iron is higher in 300g/L batch the experiment, and total concentration of iron is lower when 450g/L; Concentration was higher when solubility arsenic was 450g/L at total solid concentration, and arsenic concentration is lower when 300g/L.Show solids concn 450g/L (150g/L breeze+300g/LAl in the rotary drum bio-reactor
2O
3) batch biological leaching effect and total solid concentration be 300g/L (150g/L breeze+150g/LAl
2O
3) batch leaching effect approaching.Mean and adopt the mutagenesis mixed bacterium to leach arsenic-containing gold ore, in the arsenic-containing gold ore biological pre-oxidation is handled, important application prospects is arranged at revolving drum type bioreactor.
Claims (6)
1. one kind is adopted the biological pre-oxidation treatment method that leaches arsenic-bearing gold ore of rotating drum type reactor, and this method may further comprise the steps:
1) will use the tap water rinsing through the arsenic-containing gold concentrate of flotation;
2) arsenic-containing gold concentrate after the rinsing in the step 1) is carried out the drainage oven dry, grinds activation, sour preimpregnation;
3) will have a liking for ferrous thiobacillus of acid oxidase and the mutagenesis bacterium of having a liking for acid oxidase sulphur thiobacillus, and carry out acclimation shaking culture, amplification culture obtains the mutagenesis mixed bacterium;
4) mutagenesis mixed bacterium in the step 3) is inoculated into the high density arsenic-containing gold ore after acidleach, in rotating drum type reactor, carries out biology and leach;
5) with the arsenic-containing gold ore bio-oxidation soak slag carry out cyanidation gold-extracted, and with leach liquor through analyzing and testing and after removing harmful ion, circulation is used for the continuous leaching of arsenic-bearing gold ore;
Carry out the biological pre-oxidation of high density arsenic-containing gold ore in the described rotating drum type reactor; Directly promote mineral grain through the rotary drum interior panelling and carry out the ore pulp uniform mixing; Realize by gas distributor that gas is pre-dispersed and fully contact mixing with gas-liquid, promotion gas-liquid mass transfer and dissolved oxygen supply are abundant.
2. pre-oxidation treatment method according to claim 1 is characterized in that, described gas distributor comprises: inlet pipe (1), branch's gas tube (2), gas distribution tube (3) and escape pipe (4); Described inlet pipe (1) wears on the horizontal fixed axle that is fixed in rotary drum one end, and the outlet side that this inlet pipe (1) is positioned at rotary drum is communicated to some gas distribution tubes (3) through branch's gas tube (2); Described branch gas tube (2) semicircular in shape, some gas distribution tubes (3) are along the axially parallel setting of rotary drum; Described escape pipe (4) is arranged on the horizontal fixed axle of rotary drum one end, and its end expands part for cylindrical microporous collection and confinement of gases pipe, and this microporous collection and confinement of gases is guaranteed and is held in more than the liquid level its top end surface sealing, side perforate.
3. pre-oxidation treatment method according to claim 1 is characterized in that, in the described step 1) arsenic-containing gold ore is carried out the repeated multiple times rinsing to clarification with tap water.
4. pre-oxidation treatment method according to claim 1 is characterized in that, described step 2) at first rinsing ore drainage is placed in the loft drier, temperature-stable is 110～120 ℃; Then, the sample ore that drying is crossed grinds activation, more than the continuously grinding 30min; At last, the sample ore after grinding is carried out acidleach, the mass ratio of sample ore and pickling liquor is 1: 2 or 1: 3, and controls the pH of soak solution and be stabilized in 1.5～2.0 through adding sulphuric acid soln in the immersion process, noticeable change does not take place to pH.
5. pre-oxidation treatment method according to claim 1 is characterized in that, described step 3) may further comprise the steps:
3-1) with the uv lamp of 15～20W, wavelength 265nm, preheating 20～30min operates in camera bellows; Have a liking for the ferrous thiobacillus of acid oxidase and the petridish of having a liking for acid oxidase sulphur thiobacillus 10～20ml, diameter 9～15cm apart from uv lamp 30～40cm with filling bacterium liquid, carry out radiotreatment by different time; Bacterium liquid lucifuge was handled 12～24 hours in 4 ℃ of refrigerators after the mutagenesis, was placed on lucifuge cultivation in the shaking table, obtained the gain mutant bacterial strain of 2 kinds of enrichments respectively;
3-2), leach the continuous domestication of carrying out the mutagenesis bacterial classification through shaking bottle respectively with the mutagenic strain of 2 kinds of ore immersion bacterial culture enrichments, in 250～1000ml Erlenmeyer flask and Leathen substratum, Fe
2+Or sulphur powder amount reduces and mode that arsenic-containing gold ore powder ratio increases gradually gradually, increases arsenic-containing gold ore 2%～5% at every turn, just reduces Fe
2+Or sulphur powder amount 2%～5%, when arsenic-containing gold ore content greater than 20% the time, stop to use Fe
2+Or the sulphur powder is made the energy; Control acclimation shaking culture base pH is 1.5～2.5, and to having a liking for the cultivation of going down to posterity of ferrous thiobacillus of acid oxidase and thiobacillus thiooxidans, culture temperature is 25～35 ℃ respectively; Continuous domestication is cultivated more than five generations and is obtained concentration greater than 10
7The flexibility domestication bacterium of individual/ml;
3-3) in optimizing the Leathen substratum, and add Fe in proportion
2+And elemental sulfur, mixing amplification culture through continuous round-robin mutagenesis bacterial classification; At first carry out the batch shake-flask culture less than the Gold Ore of 75 μ m, in 10L, 20L and 50L rotary drum reactor drum, carry out amplification culture step by step then at 90% above mineral granularity; Wherein, inoculum size be the culture TV 10%～30%, temperature between 30～35 ℃, the pH value is between 1.5～2.5, adding the breeze amount is 20%～50% of culture gross weight, obtaining inoculum density is 10
7～10
9The mutagenesis mixed bacterium of high ore pulp of the adaptation of individual/ml and high ion concentration.
6. pre-oxidation treatment method according to claim 1 is characterized in that, described step 4) may further comprise the steps:
4-1) sample ore after the acidleach is joined in the rotary drum reactor drum, add leach liquor and inoculation mutagenesis mixed bacterium in proportion, the control pulp density is 20～50%, and the bacterial classification initial density is greater than 10
7Individual/ml;
4-2) leach liquor is prepared with tap water, adds nutrition composition by the Leathen substratum of optimizing, and contains (NH in the 1L substratum
4)
2SO
41.5g, MgSO
47H
2O 0.05g, KCl 0.05g, K
2HPO
40.05g, Ca (NO
3)
20.01g;
4-3) in the arsenic-containing gold ore bioleaching process, the controlling reactor temperature is that 30～35 ℃, pH value 1.5～2.0, Ventilation Rate are that 2～20L/min, rotary drum rotating speed are 1～10r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102395892A CN101899570B (en) | 2010-07-27 | 2010-07-27 | Preoxidation treating method for leaching arsenic golden ores by using drum type reactor organisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102395892A CN101899570B (en) | 2010-07-27 | 2010-07-27 | Preoxidation treating method for leaching arsenic golden ores by using drum type reactor organisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101899570A CN101899570A (en) | 2010-12-01 |
| CN101899570B true CN101899570B (en) | 2012-05-23 |
Family
ID=43225487
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102395892A Expired - Fee Related CN101899570B (en) | 2010-07-27 | 2010-07-27 | Preoxidation treating method for leaching arsenic golden ores by using drum type reactor organisms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101899570B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103014336B (en) * | 2013-01-16 | 2014-04-16 | 山东黄金归来庄矿业有限公司 | Preparation and method for treating high-arsenic and high-sulfur gold ore |
| CN106495389B (en) * | 2015-09-08 | 2019-12-03 | 铜仁市万山区盛和矿业有限责任公司 | A method of extracting arsenic from ore waste water |
| CN113122730B (en) * | 2021-04-12 | 2022-05-24 | 东北大学 | Palmaria type gold ore double-roller crushing-bacterial pool leaching oxidation gold extraction method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1206376C (en) * | 1999-09-07 | 2005-06-15 | 比利顿知识产权有限公司 | Bioleaching of sulphide minerals |
| CN101191154B (en) * | 2006-11-29 | 2010-05-12 | 中国科学院过程工程研究所 | Revolving drum type bioreactor used for microorganism lixiviation |
-
2010
- 2010-07-27 CN CN2010102395892A patent/CN101899570B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN101899570A (en) | 2010-12-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5914441A (en) | Biocatalyzed anaerobic oxidation of metal sulfides for recovery of metal values | |
| CN101538540B (en) | Composite mineral-leaching bacteria colony and use of same in biological metallurgy | |
| CN101333599A (en) | Cyanidation aurum-extracting method for preprocessing high-arsenic complex refractory gold ore by oxidation with arsenic resistant strains | |
| AU2007219367A1 (en) | Reactor for the culture, biooxidation of solutions and/or large-scale propagation of isolated microorganisms and/or native microorganisms that are useful in ore leaching | |
| CN105734285B (en) | A kind of method for strengthening zincblende Microorganism Leaching | |
| CN102260568A (en) | Combined microbial desulfurization method and process of coal | |
| CN100362117C (en) | Sulfide ore treatment technology by thermoacidophile | |
| US5030426A (en) | Biomining of gallium and germanium containing ores | |
| CN103014336B (en) | Preparation and method for treating high-arsenic and high-sulfur gold ore | |
| CN101899570B (en) | Preoxidation treating method for leaching arsenic golden ores by using drum type reactor organisms | |
| Dan et al. | Reductive leaching of manganese from manganese dioxide ores by bacterial-catalyzed two-ores method | |
| Ubaldini et al. | Combined bio-hydrometallurgical process for gold recovery from refractory stibnite | |
| CN102337402A (en) | Extraction method of gold from gold-containing sulfur ore concentrate | |
| Petersen | From understanding the rate limitations of bioleaching mechanisms to improved bioleach process design | |
| CN107352767A (en) | A kind of bioleaching hardening agent and the method for removing sludge heavy-metal | |
| CN104630467B (en) | Biological contact oxidation pond and method for oxidizing Fe2+ in dump leaching process | |
| Bai et al. | Bioleaching of heavy metals from a contaminated soil using bacteria from wastewater sludge | |
| van Niekerk | Recent advances in the BIOX® technology | |
| CN207567313U (en) | A kind of the continuous of red mud leaches bioleaching device | |
| Meng et al. | UV induced mutations in Acidianus brierleyi growing in a continuous stirred tank reactor generated a strain with improved bioleaching capabilities | |
| CN114350947A (en) | Special oxidizing bacteria and heap leaching pre-oxidation-gold extraction process for refractory gold ore organisms | |
| CN1215758A (en) | Method for bacteria preoxidation extraction of gold from refractory high-arsenic gold-ore and bacteria oxidation tank used | |
| CN103205381B (en) | Decarbonizing and desulphurizing bacterium agent for difficultly-selected gold ore and application thereof | |
| CN102618472A (en) | Method for breeding and separating high arsenic oxidizing bacteria | |
| Soleimani et al. | Microbial leaching of a low-grade sphalerite ore using a draft tube fluidized bed bioreactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120523 Termination date: 20170727 |