CN1389102A - Culture mediwn for fast propagation of kulasu aloe - Google Patents
Culture mediwn for fast propagation of kulasu aloe Download PDFInfo
- Publication number
- CN1389102A CN1389102A CN 01114501 CN01114501A CN1389102A CN 1389102 A CN1389102 A CN 1389102A CN 01114501 CN01114501 CN 01114501 CN 01114501 A CN01114501 A CN 01114501A CN 1389102 A CN1389102 A CN 1389102A
- Authority
- CN
- China
- Prior art keywords
- medium
- agar
- litre
- culture medium
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to culture media for quick breeding aloe vera, including: infant seedling differentiation induction culture medium contains MS conventional basic culture medium, 6-benzyladenine 2 mg/l, indoleacetic acid 0.1 mg/l, agar 0.65% and cane sugar 3.0%; multiplication culture medium contans MS conventional basic culture medium, 6-benzyladenine 2 mg/l, naphthaleneacetic acid 0.2 mg/l, ager 0.65% and cane sugar 3%; and rootint culture medium contains 1/2 MS conventional basic culture medium, indoleacetic acid 2.4 mg/l, agar 0.65% and cane sugar 3.0%, in which the agar and cane sugar contents are mass content. Said invention can implement industrial production.
Description
The present invention relates to medium that aloe is bred.
Aloe is widely used in aspects such as medicine material, cosmetic composition and health care of food product at present.Particularly aloe barbadensis Miller is thick owing to leaf, the output height, and the bioactive functions composition enriches and is subjected to society's attention, and demand is increasing.But because itself can't be finished sexual propagation, it is too slow to finish expansion speed by vegetative propagation (cuttage or plant division), and being carried out tissue culture, aloe barbadensis Miller can breed seedling faster, though all begin one's study both at home and abroad, really use batch production to produce still needs at technology, efficient and the enterprising step refining of filling a prescription.
Purpose of the present invention aims to provide a kind of can the breeding fast aloe barbadensis Miller, can carry out batch production production, the medium of the quick breeding aloe barbadensis Miller that process efficiency is high.
The objective of the invention is to realize by following manner:
The present invention was subdivided into for three steps with tissue culture procedures: the induction period of the bud of promptly growing thickly, fast breeding stage and culture of rootage stage, per step, corresponding medium was respectively: induce the seedling differential medium to contain the conventional minimal medium of MS, 6 benzyladenines, 2 mg/litre, heteroauxin 0.1 mg/litre, agar 0.65%, sucrose 3.0%; The proliferated culture medium in fast breeding stage contains the conventional minimal medium of MS, 6 benzyladenines, 2 mg/litre, methyl 0.2 mg/litre, agar 0.65%, sucrose 3%; Root media contains the conventional minimal medium of 1/2MS, 2.4 mg/litre heteroauxins, agar 0.65%, sucrose 3.0%, and wherein agar, sucrose all refer to mass content.
It is 5.8～6.0 that medium in per step is adjusted PH.
Below in conjunction with accompanying drawing the present invention is described in further detail.
Accompanying drawing is a process chart of the present invention.
Shown in accompanying drawing, the process of utilizing the present invention to cultivate aloe barbadensis Miller is: 1, medium joins
Shown in accompanying drawing, the process of utilizing the present invention to cultivate aloe barbadensis Miller is: 1, culture medium preparation, respectively three kinds of medium are prepared in proportion MS (Marashige﹠amp; Sroog) be conventional minimal medium.With adjusting pH value after prepared culture medium (relevant component content the sees Table I) heating for dissolving is 5.8～6.0, in the clean cylindrical bottle of packing into, seals the back high pressure sterilization under 120 ℃ of left and right sides steams about 15 minutes.
Hormone, sucrose and agar content in each stage medium of Table I
*The IBA-heteroauxin, NAA-naphthyl acid, BA-6 benzyladenine
2, explant is selected and is disposed.
The explant of select robust growth, anosis no worm, the leaf compactness, high about 30 centimetres tender stem of aloe barbadensis Miller seedling being done cultivation, clear water flush away surface irregularities, shake with saturated washing powder again and changed the clear water rinsing in 5 minutes over to and enter transfer room, sterilized 5 seconds with 75% alcohol earlier, use 0.15%HgCL immediately
2Sterilized 10 minutes, aseptic water washing 4 times is cut into the segment of 0.6 cm thick after draining, be used for inoculation.
3, inducing clumping bud is cultivated.
Explant is seeded on the differential medium, 3 sections of the every bottle graft kinds of the cylindrical bottle that diameter is 8 centimetres, condition of culture is: 26 ℃+(-) 2 ℃, irradiation 12h, light intensity 1500lx (lux).Discovery is by in time sorting out that mushroom pollutes, and is the much longer budlet that grows thickly (giving birth to about 21 around every explant) about 45 days, is used for propagation or successive transfer culture.
4, fast breeding.
The bud of will growing thickly separately changes in the proliferated culture medium, 18～25 of every blake bottle inoculations, and warm light is constant, and 25 days, leaf grew to more than the 2cm.
5, culture of rootage.
The seedling that fast breeding is cultivated changes in the root media, and at irradiation 12H, illumination 2000lx cultivates down, grow 5～6 (about 15 days) after, change hardening over to.
6, the transplanting of test-tube plantlet.
Earlier with blake bottle in normal room uncovered 3 days, flush away root medium is implanted in after drying in the seedbed of booth before transplanting.
Below will by some concrete experiment situations illustrate of the present invention for medium be the optimum proportioning for cultivating aloe barbadensis Miller.
At first be the influence of growth substance to the differentiation of test-tube plantlet bud.
In order to seek the most effective growth substance, so that evoked callus differentiates more bud, we test the kind and the concentration of growth substance, and Table II is under the situation that kinetin BA (2ppm) arranged, and bud forms situation behind adding NAA or the IBA.
Table II growth hormone is to the influence of test-tube plantlet bud differentiation
| Concentration | Growth coefficient | The bud quality |
| ????NAA0.1 | ????8-10 | Slight of stature, neat |
| ????NAA0.15 | ????14-18 | Healthy and strong, neat |
| ????NAA0.20 | ????13-17 | Healthy and strong, irregular |
| ????IBA0.05 | ????10-14 | Slight of stature, neat |
| ????IBA0.10 | ????18-24 | Healthy and strong, neat |
| ????IBA0.15 | ????18-25 | Healthy and strong, irregular |
Last table shows that IBA0.10ppm is the highest to aloe barbadensis Miller bud growth coefficient.
Be the influence of growth hormone in addition to rooting of vitro seedling.
Take root number and the survival rate of test-tube plantlet is to influence field production to survive key factor with production cost, and
Different growth hormone have difference (Table III) to this influence, and our work shows that the IBA of 2.4ppm is optimal a kind of method.
Table III growth hormone is to the influence of rooting of vitro seedling
Agar concentration is to the influence of breeding.
In the group training process, the agar usage amount is bigger, its effect only is again as nutraceutical carrier, therefore, exploring its minimum effective dose is the important measures that reduce production costs, our practice shows that 0.65% agar consumption is an economized form of carrying out the training of aloe group, individual event cost savings nearly 20%.
The Table IV agar concentration is to the influence of reproduction speed
| Concentration | The bud coefficient of differentiation | Rooting rate | Survival rate |
| ????0.60％ | ????10-16 | ????67％ | ????92％ |
| ????0.65％ | ????17-25 | ????91％ | ????92％ |
| ????0.70％ | ????17-24 | ????91％ | ????92％ |
| ????0.80％ | ????18-25 | ????90％ | ????92％ |
In sum, the present invention was subdivided into for three steps with tissue culture procedures, and per step correspondence is furnished with corresponding medium, can directly apply to batch production production, carry out refinement on technology, efficient, the prescription, have accurately quantitatively, cost is low, and reproductive speed is fast, the high characteristics of production efficiency.The first batch of production cycle is 85-95 days, and growth coefficient is about 20, and the production cycle is 60 days after the ordinary production, and has adjustability.
Claims (2)
1, breeds the medium of aloe barbadensis Miller fast, tissue culture procedures was subdivided into for three steps: the induction period of the bud of promptly growing thickly, fast breeding stage and culture of rootage stage, per step, corresponding medium was respectively: induce the seedling differential medium to contain the conventional minimal medium of MS, 6 benzyladenines, 2 mg/litre, heteroauxin 0.1 mg/litre, agar 0.65%, sucrose 3.0%; The proliferated culture medium in fast breeding stage contains the conventional minimal medium of MS, 6 benzyladenines, 2 mg/litre, methyl 0.2 mg/litre, agar 0.65%, sucrose 3%; Root media contains the conventional minimal medium of 1/2MS, 2.4 mg/litre heteroauxins, agar 0.65%, sucrose 3.0%, and wherein agar, sucrose all refer to mass content.
2, quick breeding aloe barbadensis Miller medium as claimed in claim 1, it is 5.8～6.0 that the medium in per step is adjusted PH.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01114501 CN1389102A (en) | 2001-05-31 | 2001-05-31 | Culture mediwn for fast propagation of kulasu aloe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01114501 CN1389102A (en) | 2001-05-31 | 2001-05-31 | Culture mediwn for fast propagation of kulasu aloe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1389102A true CN1389102A (en) | 2003-01-08 |
Family
ID=4661139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01114501 Pending CN1389102A (en) | 2001-05-31 | 2001-05-31 | Culture mediwn for fast propagation of kulasu aloe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1389102A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100374011C (en) * | 2003-11-07 | 2008-03-12 | 云南省农业科学院园艺作物研究所 | Method for tissue culture of lily flowers |
| CN106937598A (en) * | 2017-04-18 | 2017-07-11 | 屏南县惠荣农业科技有限公司 | The original seed preparation method of big aloe |
| CN107114094A (en) * | 2017-05-13 | 2017-09-01 | 梁钟 | A kind of method of desert area Aloe Planting |
-
2001
- 2001-05-31 CN CN 01114501 patent/CN1389102A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100374011C (en) * | 2003-11-07 | 2008-03-12 | 云南省农业科学院园艺作物研究所 | Method for tissue culture of lily flowers |
| CN106937598A (en) * | 2017-04-18 | 2017-07-11 | 屏南县惠荣农业科技有限公司 | The original seed preparation method of big aloe |
| CN106937598B (en) * | 2017-04-18 | 2019-03-26 | 屏南县惠荣农业科技有限公司 | The original seed production method of big aloe |
| CN107114094A (en) * | 2017-05-13 | 2017-09-01 | 梁钟 | A kind of method of desert area Aloe Planting |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101258835B (en) | Fast reproducing method for high quality seedling of dendrobium officinale | |
| CN104335903B (en) | It is a kind of to promote Pseudobulbus Bletillae (Rhizoma Bletillae) rapid propagation method | |
| CN102150624B (en) | Tissue culture and rapid propagation method for pinellia tuber plant | |
| CN104705188B (en) | The method for cultivating dendrobium candidum protocorm | |
| CN103270952B (en) | Sinkiang drug mulberry tissue culture and rapid propagation culture medium | |
| CN1226408C (en) | Method for obtaining haploid plant strain by culturing anther or pollen | |
| CN101953300B (en) | Tissue culture method for Curcuma wenyujin No.1 | |
| KR20090120813A (en) | Method for mass propagation of callus and method for mass division of iris odaesanensis flower utilizing leaf segments of iris odaesanensis | |
| CN103688856A (en) | Rapid propagation method of Blumea riparia (Bl.) DC medicinal material | |
| CN106106178A (en) | A kind of method for tissue culture of confection Rhizoma Iridis Tectori | |
| CN103749307B (en) | The tissue culture propagation of short lobe China pink | |
| CN113207698A (en) | Novel tissue culture seedling raising method for one-step seedling raising by utilizing polygala tenuifolia | |
| CN110741937B (en) | Rapid propagation method of polygonatum sibiricum | |
| CN104429974A (en) | Rooting culture medium for culturing dendrobium officinale plantlets | |
| CN1389102A (en) | Culture mediwn for fast propagation of kulasu aloe | |
| CN1149921C (en) | Morinda tissue culture method | |
| CN101120654B (en) | Tissue culturing method for chia | |
| CN108450328A (en) | A kind of crocodile mouth flower quick breeding method for tissue culture | |
| CN1778169A (en) | Induction of peony embryoid | |
| CN107494269A (en) | A kind of elimination little Hua all ages in blue tissue culture procedures endophyte method | |
| CN107155882A (en) | A kind of medicinal bletilla striata aseptic seeding fast seedling-cultivating method | |
| CN104823845B (en) | The method that rapid induction breeds salt SHENGCAO young root calluss | |
| CN107743868A (en) | A kind of method for efficiently breeding roxburgh anoectochilus terminal bud using nature optical culture forming seedling through one step culture | |
| CN108834893B (en) | Novel tissue culture and rapid propagation method for red-root wild broad beans | |
| CN101422134A (en) | Sargassum thunbergii young seedling large-scale breeding method using leaves tissue culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |