CN1385059A - Method for breeding isoaddition series of small ice-age wheat and translocation series - Google Patents
Method for breeding isoaddition series of small ice-age wheat and translocation series Download PDFInfo
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- CN1385059A CN1385059A CN 01119170 CN01119170A CN1385059A CN 1385059 A CN1385059 A CN 1385059A CN 01119170 CN01119170 CN 01119170 CN 01119170 A CN01119170 A CN 01119170A CN 1385059 A CN1385059 A CN 1385059A
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- wheatgrass
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Abstract
The present invention relates to a method for breeding superior variety of wheat, and is characterized that firstly, plant chromosome engineering is used to create two sets of different additional series of small ice-age wheat (14 materials), then create translocation line, then the translocation line with excellent agronomic character is selected and hybridized with wheat so as to breed out high-quality wheat variety-small ice-age wheat 33, small ice-egg wheat 32 and small ice-age wheat 35.
Description
The present invention is the breeding method of wheat improved seeds
In the conventional breeding of wheat, because the gene pool poorness of wheat, the method for intervarietal cross seed selection does not go out desirable kind, therefore uses the important channel that becomes the cultivation wheat breed with the heterologous gene improvement wheat of the close kind of wheat affiliation.The gene that economic worth is arranged for the improvement wheat because have in some sibling specieses of wheat belong to that many wheats itself do not possess, for example high protein, gene such as disease-resistant.Shifting heterologous gene to wheat has various approach, and wherein the research of chromosome engineering is subjected to extensively paying attention to both at home and abroad.
In order to obtain superpower gluten flour, cultivation can be made the wheat breed of bread, special development the present invention.
We utilize the chromosome engineering method is couchgrass in the middle of sky blue wheatgrass (Agropyron intermedium) also claims in order to the wildlife species of improvement wheat, be the perennial gramineous plants (2n=42) of the nearly edge of wheat, have high protein, good characteristic such as disease-resistant, drought-enduring, cold-resistant.
In order to reach the purpose of improvement wheat, with identify through us have in sky blue wheatgrass blood relationship far away 1, far away in 2, far away in 3, far away in 4 and far away in 5 five allooctaploids be that parent material and wheat are backcrossed, they also contain 14 sky blue wheatgrass chromosomes except that containing 42 chromosomes of wheat.At first set up the series material of the additional a pair of sky blue wheatgrass of wheat, i.e. 14 alien addition lines.On this basis, formulated translocation line again.Select the good translocation line of economical character, use that the method that chromosome engineering combines with conventional breeding is cultivated high-quality, resisted more, the new variety of wheat of high yield, i.e. Xiaobingmai 33, Xiaobingmai 32 and Xiaobingmai 35.
The first step, use the allooctaploid contain sky blue wheatgrass far away in 1, far away in 2, far away in 3, far away in 4 and far away in 5 be material.At first carry out chromosomal conformational analysis, identify far away in 1 and far away in 2 contain the same group chromosome of sky blue wheatgrass; In far away 3, far away in 4 and far away in 5 contain another group chromosome of sky blue wheatgrass.With them is the parent, backcrosses with wheat, selects the additional chromosomal disome alien addition line of a pair of sky blue wheatgrass except that 42 chromosomes of wheat from its offspring, obtains 14 alien addition line materials altogether.
Second step, creation translocation line.In the alien addition line row of initiative, select and identify translocation line, the translocation line that the chromosome segment that promptly carries the useful gene of sky blue wheatgrass (high protein, rust-proofing disease etc.) translocates on the chromosome of wheat to be obtained, and identify that through RAPD, FISH and RFLP equimolecular biology techniques it is a translocation line.
The 3rd step, utilize the translocation line and the wheat hybridizing of economical character good (tool high protein, rust-proofing disease etc.), cultivate new variety of wheat-Xiaobingmai 33, Xiaobingmai 32 and the Xiaobingmai 35 of high-quality, many anti-, high yields through systematic breeding.
Their principal character: Xiaobingmai 33 its crude protein (butt) are 17.67%, rust-proofing disease, high yield (the irrigated land hectare is crossed ten thousand jin, and it is 6 very heavy that drought is planted hectare), and precocity in 94 days maturing stages, does not have fringe germination phenomenon.
Xiaobingmai 32 its crude protein (butt) are 16.60%, the rust-proofing disease, and output is close with Xiaobingmai 33, precocity, the maturing stage is 91 days, does not have fringe germination phenomenon.
Xiaobingmai 35 its crude protein (butt) are 14.5%, rust-proofing, are the beer wheat.
At first to five allooctaploids far away in 1, far away in 2, far away in 3, far away in 4 and far away in 5 carry out the analysis of chromosome configuration.The result be far away in 3, far away in 4 and far away in 5 entrained be the chromosome of same group of sky blue wheatgrass; In far away 1 and far away in 2 carry the chromosome that another organizes sky blue wheatgrass.
With the octoploid Xiaobingmai far away in 2 parents for the initiative first cover wheat-wheatgrass alien addition line, and in far away 3, far away in 4 and far away in 5 parents for the initiative second cover wheat-wheatgrass alien addition line.
With 2 backcrossing with wheat of Neoma Foam in far away, initiative first seven of the alien addition lines of cover are dissimilar.With in far away 3, far away in 4 and far away in 5 with No. 1, wheat breed drop of water city, cultivate 149,3B-2 backcrosses, initiative second seven of the alien addition lines of cover are dissimilar, obtain 14 alien addition line row materials altogether.
5 in parent's the method for backcrossing during we 1-in adopting far was far away, and we use with universal method is different in the world, and the short-cut method of promptly only backcrossing once obtains alien addition line, has shortened the time that alien addition line is listed as that obtains.In addition, this also is most complete up to now two cover alien addition line materials.Adopt the method for crisscrossing to obtain 14 alien addition line row materials that carry the coloured differently body again.
3. use RAPD and FISH technical appraisement translocation line.
In the RAPD technology, in the analysis of using a large amount of primer to do, the result that primer OPA-09 draws proves that Xiaobingmai 33 is translocation lines, after also be confirmed method advanced person, accurate again with FISH technology and RFLP technology.
Claims (1)
1, the breeding method of a kind of wheat-wheatgrass alien addition line and translocation line, it is characterized in that: with the method for chromosome engineering, with the nearly edge wild species of wheat, has high protein, disease-resistant, drought-enduring, the cold-resistant sky blue wheatgrass improvement of the perennial gramineous plants wheat that waits merit, at first to the allooctaploid of sky blue wheatgrass far away in 1, in far away 2, in far away 3, in far away 4 and far away in 5 carry out the chromosome configuration analysis, select contain same group of sky blue wheatgrass chromosomal far away in 3, in far away 4 and far away in 5 and contain another organize sky blue wheatgrass chromosomal far away in 1 and far away in 2, be that parent and wheat are backcrossed with them; With 2 backcrossing in far away with wheat, obtain a cover alien addition line, promptly be with 42 chromosomes of wheat, add chromosomal seven the dissimilar alien addition lines of a pair of sky blue wheatgrass; With in far away 3, far away in 4 and far away in 5 backcross with wheat, seven of obtaining the second cover alien addition line are dissimilar, obtain 14 alien addition line row materials altogether; In the initiative process, adopt the short-cut method of backcrossing once, adopt the method for crisscrossing again, obtain to carry 14 alien addition line row materials of coloured differently body; From these alien addition lines, select and the evaluation translocation line through phenotypic evaluation and with RAPD, FISH and RFLP equimolecular biology techniques, select good translocation line of economical character and wheat hybridizing again, through systematic breeding, cultivate the wheat breed of good quality and high output.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01119170 CN1385059A (en) | 2001-05-14 | 2001-05-14 | Method for breeding isoaddition series of small ice-age wheat and translocation series |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01119170 CN1385059A (en) | 2001-05-14 | 2001-05-14 | Method for breeding isoaddition series of small ice-age wheat and translocation series |
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| CN1385059A true CN1385059A (en) | 2002-12-18 |
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| CN 01119170 Pending CN1385059A (en) | 2001-05-14 | 2001-05-14 | Method for breeding isoaddition series of small ice-age wheat and translocation series |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423867B (en) * | 2008-07-03 | 2012-01-11 | 河北农业大学 | Method for identifying celery cabbage-brassica oleracea alien addition lines |
| CN102907316A (en) * | 2012-10-31 | 2013-02-06 | 中国农业科学院作物科学研究所 | Method for efficiently inducing wheat-wheatgrass non-homologous chromosomes to translocate |
-
2001
- 2001-05-14 CN CN 01119170 patent/CN1385059A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423867B (en) * | 2008-07-03 | 2012-01-11 | 河北农业大学 | Method for identifying celery cabbage-brassica oleracea alien addition lines |
| CN102907316A (en) * | 2012-10-31 | 2013-02-06 | 中国农业科学院作物科学研究所 | Method for efficiently inducing wheat-wheatgrass non-homologous chromosomes to translocate |
| CN102907316B (en) * | 2012-10-31 | 2016-02-17 | 中国农业科学院作物科学研究所 | The method of efficient inducing wheat-wheatgrass chromosome nonhomologous transposition |
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