CN1380301A - Ribonucleic acid extract, its production method and application - Google Patents
Ribonucleic acid extract, its production method and application Download PDFInfo
- Publication number
- CN1380301A CN1380301A CN 01113523 CN01113523A CN1380301A CN 1380301 A CN1380301 A CN 1380301A CN 01113523 CN01113523 CN 01113523 CN 01113523 A CN01113523 A CN 01113523A CN 1380301 A CN1380301 A CN 1380301A
- Authority
- CN
- China
- Prior art keywords
- weight parts
- phenol
- liquid
- ribonucleic acid
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920002477 rna polymer Polymers 0.000 title claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 9
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000000843 powder Substances 0.000 claims abstract description 23
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 22
- SOIFLUNRINLCBN-UHFFFAOYSA-N ammonium thiocyanate Chemical compound [NH4+].[S-]C#N SOIFLUNRINLCBN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000011187 glycerol Nutrition 0.000 claims abstract description 11
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 34
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 10
- 239000011734 sodium Substances 0.000 claims description 10
- 229910052708 sodium Inorganic materials 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 abstract 1
- 235000017281 sodium acetate Nutrition 0.000 abstract 1
- 239000001632 sodium acetate Substances 0.000 abstract 1
- 238000000605 extraction Methods 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 239000000203 mixture Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- JWAZRIHNYRIHIV-UHFFFAOYSA-N 2-naphthol Chemical compound C1=CC=CC2=CC(O)=CC=C21 JWAZRIHNYRIHIV-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- QWVGKYWNOKOFNN-UHFFFAOYSA-N o-cresol Chemical compound CC1=CC=CC=C1O QWVGKYWNOKOFNN-UHFFFAOYSA-N 0.000 description 2
- FTDXCHCAMNRNNY-UHFFFAOYSA-N phenol Chemical compound OC1=CC=CC=C1.OC1=CC=CC=C1 FTDXCHCAMNRNNY-UHFFFAOYSA-N 0.000 description 2
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 1
- 229950011260 betanaphthol Drugs 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- -1 naphthyl alcohol Chemical compound 0.000 description 1
Abstract
The present invention discloses a ribonucleic acid extract, its production method and application. It is formed from phenol, Guanidine Thiocyanate powder, Ammonium Thiocyanate powder, Sodium Acetate liquor, glycerin and water. Said invention can effectively extract ribonucleic acid, its extracting effect is good, and its production method is simple and convenient.
Description
The present invention relates to a kind of extraction of Yeast Nucleic Acid.
Yeast Nucleic Acid in the prior art (RNA), its leaching process complexity, extraction effect is undesirable.
The object of the present invention is to provide a kind of extracting ribonucleic acid that makes things convenient for, and good ribonucleic acid extract and production method and the purposes of extraction rate was acquired.
Technical solution of the present invention is:
A kind of ribonucleic acid extract, form by following component:
Phenol 100 weight parts
First powder 20～40 weight parts
Second liquid 20～40 weight parts
Water 160～250 weight parts
Wherein: the first powder is made up of Guanidine Thiocyanate pulvis 55～85% weight parts and Ammonium Thiocyanate pulvis 15～45% weight parts; Second liquid is made up of Sodium Acetate5～30% weight part of 2～3M and 20%～70% glycerine 70～95% weight parts.
Phenol is phenol.Phenol Phenol concentration 99+%, molecular formula C
6H
6O, molecular weight Fw94.11.
A kind of production method of ribonucleic acid extract comprises the following steps:
1. phenol 100 weight parts are dissolved in 60～80 ℃ of water-baths;
2. Guanidine Thiocyanate pulvis 55～85% weight parts and AmmoniumThiocyanate pulvis 15～45% weight parts are mixed, make the first powder; First powder 20～40 weight parts are added in the phenol that dissolves, stir;
3. Sodium Acetate5～30% weight part of 2～3M and 20%～70% glycerine 70～95% weight parts are mixed, make second liquid; Second liquid 20～40 weight parts are added in the liquid that 2. step obtains;
4. in the liquid that 3. obtains through step, add entry 160～250 weight parts.
Phenol is phenol.Phenol Phenol concentration 99+%, molecular formula C
6H
6O, molecular weight Fw94.11.
Above-mentioned ribonucleic acid extract is as the application of extracting ribonucleic acid.
The present invention can be effective to extracting ribonucleic acid, and extraction rate was acquired is good, and production method is simple, convenient.
The invention will be further described below in conjunction with embodiment.
Embodiment 1:
1. with Phenol,Solid Phenol concentration 99% (molecular formula C
6H
6O, molecular weight Fw94.11) 100 weight parts dissolve in 60～80 ℃ of (60 ℃, 70 ℃, 80 ℃ of examples) water-baths;
2. Guanidine Thiocyanate pulvis 57% weight part and AmmoniumThiocyanate pulvis 43% weight part are mixed, make the first powder; First powder 40 weight parts are added in the phenol that dissolves, stir;
3. glycerine 93% weight part with the Sodium Acetate7% weight part and 20%～70% (example 20%, 30%, 40%, 50%, 60%, 70%) of 2～3M (routine 2M, 2.5M, 3M) mixes, and makes second liquid; Second liquid 20 weight parts are added in the liquid that 2. step obtains;
4. in the liquid that 3. obtains through step, add entry 160 weight parts, promptly get product Yeast Nucleic Acid (RNA) extract.
Above-mentioned extract (Riso liquid) is made up of following composition:
Phenol 100 weight parts
First powder 40 weight parts
Second liquid 20 weight parts
Water 160 weight parts
Wherein: the first powder is made up of Guanidine Thiocyanate pulvis 57% weight part and Ammonium Thiocyanate pulvis 43% weight part; Second liquid is made up of the Sodium Acetate7% weight part of 2～3M and 20%～70% glycerine 93% weight part.
Embodiment 2:
1. with phenol Phenol concentration 99.5% (molecular formula C
6H
6O, molecular weight Fw94.11) 100 weight parts dissolve in 60～80 ℃ of (60 ℃, 70 ℃, 80 ℃ of examples) water-baths;
2. Guanidine Thiocyanate pulvis 75% weight part and AmmoniumThiocyanate pulvis 25% weight part are mixed, make the first powder; First powder 30 weight parts are added in the phenol that dissolves, stir;
3. glycerine 82% weight part with the Sodium Acetate18% weight part and 20%～70% (example 20%, 30%, 40%, 50%, 60%, 70%) of 2～3M (routine 2M, 2.5M, 3M) mixes, and makes second liquid; Second liquid 30 weight parts are added in the liquid that 2. step obtains;
4. in the liquid that 3. obtains through step, add entry 210 weight parts, promptly get product Yeast Nucleic Acid (RNA) extract.
Above-mentioned extract (Riso liquid) is made up of following composition:
Phenol 100 weight parts
First powder 30 weight parts
Second liquid 30 weight parts
Water 210 weight parts
Wherein: the first powder is made up of Guanidine Thiocyanate pulvis 75% weight part and Ammonium Thiocyanate pulvis 25% weight part; Second liquid is made up of the Sodium Acetate18% weight part of 2～3M and 20%～70% glycerine 82% weight part.
Embodiment 3:
1. with phenol Phenol concentration 99.9% (molecular formula C
6H
6O, molecular weight Fw94.11) 100 weight parts dissolve in 60～80 ℃ of (60 ℃, 70 ℃, 80 ℃ of examples) water-baths;
2. Guanidine Thiocyanate pulvis 83% weight part and AmmoniumThiocyanate pulvis 17% weight part are mixed, make the first powder; First powder 20 weight parts are added in the phenol that dissolves, stir;
3. glycerine 71% weight part with the Sodium Acetate29% weight part and 20%～70% (example 20%, 30%, 40%, 50%, 60%, 70%) of 2～3M (routine 2M, 2.5M, 3M) mixes, and makes second liquid; Second liquid 40 weight parts are added in the liquid that 2. step obtains;
4. in the liquid that 3. obtains through step, add entry 250 weight parts, promptly get product Yeast Nucleic Acid (RNA) extract.Above-mentioned extract (Riso liquid) is made up of following composition:
Phenol 100 weight parts
First powder 20 weight parts
Second liquid 40 weight parts
Water 250 weight parts
Wherein: the first powder is made up of Guanidine Thiocyanate pulvis 83% weight part and Ammonium Thiocyanate pulvis 17% weight part; Second liquid is made up of the Sodium Acetate29% weight part of 2～3M and 20%～70% glycerine 71% weight part.
Embodiment 4:
Phenol in the foregoing description 1,2,3 is replaced to naphthyl alcohol or 2-Naphthol, Resorcinol, Ortho Cresol, and all the other are all identical, promptly form new embodiment.
The present invention can be used for extracting ribonucleic acid (RNA), during concrete extracting, the extract (Riso liquid) that makes through above-mentioned steps can be added in the tissue of grind up shape, add-on is 3～20 times (3,6,9,12,16,20 times of examples) of tissue mass, again through homogenate, centrifugal treating, get supernatant, and the chlorine that adds supernatant liquor about 1/5 is walked back and forth, centrifugal treating is got supernatant again, adds the Virahol of 1/2 left and right sides volume, the centrifugal throw out that gets, promptly get RNA, can further clean, and be suspended in the DEPC treating water with 70% ethanol.
Claims (5)
1, a kind of ribonucleic acid extract is characterized in that: be made up of following component:
Phenol 100 weight parts
First powder 20～40 weight parts
Second liquid 20～40 weight parts
Water 160～250 weight parts
Wherein: the first powder is made up of Guanidine Thiocyanate pulvis 55～85% weight parts and Ammonium Thiocyanate pulvis 15～45% weight parts; Second liquid is made up of Sodium Acetate5～30% weight part of 2～3M and 20%～70% glycerine 70～95% weight parts.
2, ribonucleic acid extract according to claim 1 is characterized in that: phenol is phenol.
3, a kind of production method of ribonucleic acid extract is characterized in that: comprise the following steps:
1. phenol 100 weight parts are dissolved in 60～80 ℃ of water-baths;
2. Guanidine Thiocyanate pulvis 55～85% weight parts and AmmoniumThiocyanate pulvis 15～45% weight parts are mixed, make the first powder; First powder 20～40 weight parts are added in the phenol that dissolves, stir;
3. the Sodium Acetate5～30% weight powder of 2～3M and 20%～70% glycerine 70～95% weight parts are mixed, make second liquid; Second liquid 20～40 weight parts are added in the liquid that 2. step obtains;
4. in the liquid that 3. obtains through step, add entry 160～250 weight parts.
4, the production method of ribonucleic acid extract according to claim 3 is characterized in that: phenol is phenol.
5, the described ribonucleic acid extract of a kind of claim 1 is as the application of extracting ribonucleic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113523 CN1122041C (en) | 2001-04-10 | 2001-04-10 | Ribonucleic acid extract, its production method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113523 CN1122041C (en) | 2001-04-10 | 2001-04-10 | Ribonucleic acid extract, its production method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1380301A true CN1380301A (en) | 2002-11-20 |
| CN1122041C CN1122041C (en) | 2003-09-24 |
Family
ID=4660244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01113523 Expired - Fee Related CN1122041C (en) | 2001-04-10 | 2001-04-10 | Ribonucleic acid extract, its production method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1122041C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319213B (en) * | 2007-06-07 | 2010-10-20 | 上海市血液中心 | Bacteria genome DNA extraction liquid, preparation and application thereof |
| CN102505012A (en) * | 2011-12-30 | 2012-06-20 | 珠海出入境检验检疫局检验检疫技术中心 | Lysis solution for extracting animal virus ribonucleic acid (RNA) |
-
2001
- 2001-04-10 CN CN 01113523 patent/CN1122041C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319213B (en) * | 2007-06-07 | 2010-10-20 | 上海市血液中心 | Bacteria genome DNA extraction liquid, preparation and application thereof |
| CN102505012A (en) * | 2011-12-30 | 2012-06-20 | 珠海出入境检验检疫局检验检疫技术中心 | Lysis solution for extracting animal virus ribonucleic acid (RNA) |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1122041C (en) | 2003-09-24 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: BAIAO BIOLOGICAL TECHNOLOGY( NANTONG ) CO., LTD. Free format text: FORMER OWNER: ZHU YUANYUAN Effective date: 20060630 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20060630 Address after: 226009 five floor, hi tech innovation center, Nantong economic and Technological Development Zone, Jiangsu Patentee after: Baiao Biotechnology(Nantong ) Co., Ltd. Address before: 226006, room 2, building 44, 103 Peach Road, Nantong, Jiangsu Patentee before: Zhu Yuanyuan |
|
| C56 | Change in the name or address of the patentee |
Owner name: BIOMICS BIOISYSTECH CO., LTD. Free format text: FORMER NAME: BIOMICS BIOTECHNOLOGY ( NANTONG ) CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: Jiangsu city in Nantong Province Economic and Technological Development Zone No. 76 Chang Xing Lu Patentee after: Biomics Biotechnologies Co., Ltd. Address before: Building five, hi tech innovation center, Nantong economic and Technological Development Zone, Jiangsu Patentee before: Baiao Biotechnology(Nantong ) Co., Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20030924 Termination date: 20190410 |
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| CF01 | Termination of patent right due to non-payment of annual fee |