CN1380006A - Production method of fruit oligose sweetening agent - Google Patents
Production method of fruit oligose sweetening agent Download PDFInfo
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- CN1380006A CN1380006A CN01110654A CN01110654A CN1380006A CN 1380006 A CN1380006 A CN 1380006A CN 01110654 A CN01110654 A CN 01110654A CN 01110654 A CN01110654 A CN 01110654A CN 1380006 A CN1380006 A CN 1380006A
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- sweetening agent
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- fruit oligose
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Abstract
The production method of fruit oligosaccharide sweetening agent includes the following steps: utilizing hollow cellulose membrane to recover fermenting liquor thallus containing beta-invertase, and solidifying it and making it into biological reactor; placing cane sugar solution into the biological reactor to make conversion reaction so as to obtain the invented required fruit oligosaccharide sweetening agent. Said invention is simple in production process, low in cost, its thallus can be recovered, so that it can be continuously used for a long period.
Description
The present invention is a kind of manufacture method of fruit oligose sweetening agent, and the hollow fibre filament membrane bioreactor is made in its main Li Yong Mandatory Pseudomonas mycelium immobilization, is used for making the manufacture method of fruit oligose sweetening agent.
As everyone knows, the few candy of fruit has sweet taste, low the upper and lower teeth not meeting properly erosion property, indigestibility and the characteristic that is difficult for causeing fat, and can effectively promote probiotics in the human intestines-two fork milk-acid bacterias activation and proliferation, promote indirectly vitamins B group's synthetic, lactic acid secretion, suppress spoilage organism and prevent constipation etc., in addition, metabolism also has the influence in good front to ester matter, can reduce cholesterol in serum, Witepsol W-S 55 and phosphide.The few candy of fruit not only can use separately, and can extensively make an addition in beverage, heath food, baby milk powder, medicine, the feed, and the business opportunity potentiality are very huge.The few candy of fruit extensively is present in the natural phant, as banana, asparagus, onion and burdock etc., with the supply people for a long time for the pursuit of good health and a long life, promptly so-called from ancient times " if long-living, Chang Qing in the abdomen.If want long-lived, intestines do not have dregs " proverb.
Tradition is made the method for the few candy of fruit, be with high concentration sucrose solution (10-60%), adjust the pH value, add the sweet enzyme of β-fructofuranose again, and with temperature maintenance after 40-60 ℃, carry out catalyzed reaction, after the reaction, heating loses activity the sweet enzyme of β-fructofuranose, and then through decolouring, after the steps such as desalination, obtain one and contain glucose, fructose, sucrose and the few candy (GF2 of fruit, GF3, the GF4 composition), as described in U.S. Pat 4774183 and the clear 56-154967 of Japanese Patent, its molecular structure is mainly fructose with straight chain B-1, the 1-Kestose of 2 be combined intos (GF2), the few candy mixture of the reacted fruit of Nystose (GF3) and 1-β-FructofuranosylNystose (GF4), its structure be as shown below:
Its sugariness is about 60% of sucrose, has the good matter mouthfeel as sucrose, the characteristic in food-processing: also all suitable with sucrose as viscosity, water activity, moisture retention etc.
The object of the present invention is to provide a kind of manufacture method of fruit oligose sweetening agent, utilization contains the sweet Mei De of β-fructofuranose Mandatory Pseudomonas mycelium and is solidified, make hollow fibre filament product recovery membrane liquid bacterium, immobilization in addition again, make bio-reactor, reach the purpose that the few candy of fruit is produced in continuous manufacturing.
The object of the present invention is achieved like this: a kind of manufacture method of fruit oligose sweetening agent is characterized in that: utilize the tubular fibre cortina to reclaim and contain the sweet enzymic fermentation liquid of β-fructofuranose thalline, wash with deionized water; Pass through wherein with the polyethylene imido again,, this tubular fibre cortina is solidified into bio-reactor with the deionized water washing; The sucrose solution that will be dissolved in again in the acetate buffer solution flows in this bio-reactor, and carries out conversion reaction under constant temperature, makes fruit oligose sweetening agent of the present invention.
The percent by weight of this polyethylene imido is 1-3%.This hollow fibre filament film thickness is 1-3cm.The speed that this sucrose solution flows through bio-reactor is 40-60ml/min.
Major advantage of the present invention is a method of the present invention, its thalline reclaims and being made as once of immobilization bioreactor finished, and continuous life-time service, being different from general traditional method is to carry out continuously, has to make effect simple and convenient, with low cost and that can use continuously for a long time.
Describe in detail below in conjunction with preferred embodiment and accompanying drawing.
Fig. 1 is a manufacturing process synoptic diagram of the present invention.
Fig. 2 is a device synoptic diagram of the present invention.
Fig. 3 is that the present invention is the synoptic diagram that the sugar in the reaction product of matrix is formed with different concns sucrose.
Fig. 4 is the high speed liquid chromatography spectrogram of the few candy of fruit of the present invention, sucrose and glucose.
Fig. 5 is the result schematic diagram that the present invention carries out the continuous production test.
Consult Fig. 1-shown in Figure 2, the manufacture method of fruit oligose sweetening agent of the present invention, it mainly is divided into two steps:
First step, with hollow fibre filament product recovery membrane liquid thalline, and immobilization in addition, be made into bio-reactor 1;
Second step is made the few candy of production fruit continuously with bio-reactor.
Its idiographic flow is as follows: Mandatory Pseudomonas mycelium bacterial classification is made fermentation culture earlier, again with after the pressure filtration of tubular fibre cortina, mycelium is reclaimed, wash with deionized water; Make the polyethylene imido flow through the tubular fibre cortina, with the deionized water washing, the tubular fibre cortina can be solidified into bio-reactor 1 again; Again with sucrose solution 22 from sucrose solution storage tank 2 with certain flow rate from the inlet 11 of bio-reactor 1 by bio-reactor, after the reaction, enter the few candy product storage tank 3 of fruit from the outlet 12 of bio-reactor 1, so, can obtain required fruit oligose sweetening agent.The import and export 13,14 of this bio-reactor 1 by thermostatic medium is connected (not shown) with thermostat, and maintenance bio-reactor 1 is used under constant temperature.
Be described as follows below by specific embodiment:
First step: the Mandatory Pseudomonas mycelium strain fermentation nutrient solution 200ml with suspending contains the about 14g of weight in wet base and flows through the tubular fibre cortina pressure filtration that thickness is 1-3cm, and washes with the deionized water of 600ml;
Second step: transferring pH is 5.0, concentration expressed in percentage by weight is that the polyethylene imido 600ml of 1-3% flows through the tubular fibre cortina, reclaims the mycelium ferment that has absorbed the polyethylene imido, washs with the flushing of 600ml deionized water again, and after being filtered, the tubular fibre cortina is solidified into bio-reactor;
Third step: with the sucrose solution of 2000ml 50% (w/v), with flow velocity 40-60ml/min, flow in the bio-reactor, and under 50 ℃, react, the activity that records the sweet enzyme of β-fructofuranose in this reactor is 35000 units, and the rate of recovery of thalline ferment is then greater than 95%.
The active method of the sweet enzyme of above-mentioned test thalline β-fructofuranose is as follows: with ferment solution or the bacterial culture fluid behind homogeneous, after suitably diluting, the sucrose solution of getting 0.5ml adding 50% (w/v) is dissolved in the acetate buffer solution of PH5.0 and 0.05M, get 3ml in 50 ℃ the reaction 1 hour after, in boiling water, heated 10 minutes again, stop the ferment reaction, with reaction solution after suitable dilution, with composition in the HPLC analytical reaction liquid, the result, the activity of the sweet enzyme of β-fructofuranose, under above-mentioned reaction conditions, per minute generates the ferment amount of the 1-Kestose (molecular weight 504) of 1 μ mole.
Consult Fig. 3-Fig. 4, sucrose solution with acetate buffer solution preparation 30%, 40%, 50%, 60% different concns such as (w/v), under 50 ℃, after carrying out conversion reaction in the flow velocity feeding bio-reactor with 40-60ml/min, sugar in products therefrom composition is analyzed with high speed liquid chromatography (HPLC), and its result as shown in Figures 3 and 4.
Consult Fig. 5, reactor of the present invention need not changed, can generate the few candy of required fruit continuously always, now prepare the sucrose solution of 50% (W/v) with acetate buffer solution, under 40-45 ℃, flow in the bio-reactor of the present invention, carry out successive reaction, operate continuously 60 days, the few candy of its fruit generates the result as shown in Figure 5.
In sum, the present invention has successive and uses effect, and has the utility value on the industry; the present invention has novelty and creativeness; for the personnel that are familiar with this skill, its detail structure is partly carried out the variation of various equivalences, all should be included within protection scope of the present invention.
Claims (4)
1, a kind of manufacture method of fruit oligose sweetening agent is characterized in that: utilize the tubular fibre cortina to reclaim and contain the sweet enzymic fermentation liquid of β-fructofuranose thalline, wash with deionized water; Pass through wherein with the polyethylene imido again,, this tubular fibre cortina is solidified into bio-reactor with the deionized water washing; The sucrose solution that will be dissolved in again in the acetate buffer solution flows in this bio-reactor, and carries out conversion reaction under constant temperature, makes fruit oligose sweetening agent of the present invention.
2, the manufacture method of fruit oligose sweetening agent as claimed in claim 1 is characterized in that: the percent by weight of this polyethylene imido is 1-3%.
3, the manufacture method of fruit oligose sweetening agent as claimed in claim 1 is characterized in that: this hollow fibre filament film thickness is 1-3cm.
4, the manufacture method of fruit oligose sweetening agent as claimed in claim 1 is characterized in that: the speed that this sucrose solution flows through bio-reactor is 40-60ml/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011106549A CN1236697C (en) | 2001-04-13 | 2001-04-13 | Production method of fruit oligose sweetening agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011106549A CN1236697C (en) | 2001-04-13 | 2001-04-13 | Production method of fruit oligose sweetening agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1380006A true CN1380006A (en) | 2002-11-20 |
| CN1236697C CN1236697C (en) | 2006-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011106549A Expired - Fee Related CN1236697C (en) | 2001-04-13 | 2001-04-13 | Production method of fruit oligose sweetening agent |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492610A (en) * | 2011-12-19 | 2012-06-13 | 南宁纵联科技有限公司 | Method for preparing fructo-oligosaccharide by utilizing molecular sieve and baffle bioreactor |
| CN110904079A (en) * | 2020-01-07 | 2020-03-24 | 中国科学院天津工业生物技术研究所 | β -fructofuranosidase mutant, mutant gene and application thereof in preparation of vitamin B12In (1) |
-
2001
- 2001-04-13 CN CNB011106549A patent/CN1236697C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492610A (en) * | 2011-12-19 | 2012-06-13 | 南宁纵联科技有限公司 | Method for preparing fructo-oligosaccharide by utilizing molecular sieve and baffle bioreactor |
| CN102492610B (en) * | 2011-12-19 | 2013-09-11 | 南宁纵联科技有限公司 | Method for preparing fructo-oligosaccharide by utilizing molecular sieve and baffle bioreactor |
| CN110904079A (en) * | 2020-01-07 | 2020-03-24 | 中国科学院天津工业生物技术研究所 | β -fructofuranosidase mutant, mutant gene and application thereof in preparation of vitamin B12In (1) |
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| Publication number | Publication date |
|---|---|
| CN1236697C (en) | 2006-01-18 |
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