CN1377688A - Extracting process for decoction of poria, Bighead Atractylodes, cinnamom and Lirorice and its medicinal use - Google Patents
Extracting process for decoction of poria, Bighead Atractylodes, cinnamom and Lirorice and its medicinal use Download PDFInfo
- Publication number
- CN1377688A CN1377688A CN 02109499 CN02109499A CN1377688A CN 1377688 A CN1377688 A CN 1377688A CN 02109499 CN02109499 CN 02109499 CN 02109499 A CN02109499 A CN 02109499A CN 1377688 A CN1377688 A CN 1377688A
- Authority
- CN
- China
- Prior art keywords
- extraction
- decoction
- linggui zhugan
- zhugan decoction
- myocardial ischemia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides an extracting process of poria, cinnamom, largehead atractylodes and licorice decoction and the process is optimized through orthogonal design. In the recipe, the ratio of poria, cinnamom, largehead atractylodes and licorice is 12 to 9 to 9 to 6, and the medicine materials are solvent extracted. The selected solvent includes water and ethyl alcohol solution in different concentration and the extraction process may be either one including first water extraction and ethyl alcohol precipitation or one including first ethyl alcohol extraction and water precipitation. The present invention may be used in preparing medicine for treating myocardial ischemia and arrhythmia.
Description
Technical field:
The present invention relates to medical technical field, exactly it is a kind of linggui zhugan decoction extraction process and medical usage thereof.
Background technology:
Zhang Zhongjing at first extracts " phlegm retention " name of disease in " Medical Treasures of the Golden Chamber ", and formulated the linggui zhugan decoction of controlling drink from spleen, we see " typhoid fever " place, " gold is deficient " two places, 67 of " the typhoid fever sun " pieces of writing: " if typhoid fever is if tell down epigastric fullness, upward adverse flow of QI to the chest; dizziness when getting up, deep and tense pulse.Diaphoresis is influencing meridian then, trembling and shaking of the body person Poria Ramulus Cinnamomi Rhizoma Atractylodis Macrocephalae glycyrrhiza decoction master it "." golden deficient cough due to fluid-retention abnormal pulse card control " cloud: " under the heart phlegm retention is arranged, distention and fullness in the chest and hypochondrium, dizzy, the linggui zhugan decoction master it ".Again cloud " husband's short breath with mild fluid-retention, when going it from urine, the linggui zhugan decoction master it " [Wang Zhanxi chief editor, Zhang Zhongjing medicine method research (1984, Beijing scientific and technical literature publishing house), pp.560-563].The side is made up of Poria, Ramulus Cinnamomi, the Rhizoma Atractylodis Macrocephalae and Radix Glycyrrhizae four flavor conventional Chinese medicines, and wherein the Poria invigorating the spleen to clear away damp pathogen is with diuretic; The Ramulus Cinnamomi activating YANG and prormoting functioning of QI, resolving fluid-retention with drugs of warm nature; The strong out-of-the-way dampness of the Rhizoma Atractylodis Macrocephalae is with the source of the living expectorant of dispelling; The Radix Glycyrrhizae invigorating the spleen and replenishing QI, coordinating the actions of various ingredients in a prescription.Four medicines share, and become the prescription commonly used [Zhou Fengwu chief editor, the practical pharmacology of Chinese medical formulae (1989, Shandong science tech publishing house), pp.551-553] of treatment phlegm retention.Yet up to now, research to the compound recipe linggui zhugan decoction mostly is its plus-minus side and clinical practice etc., and the report aspect extraction process and pharmacodynamics is less, Fu Yanling, Zhang Wei the people such as build and this side have been done partly [the Fu Yanling etc. that work to cardiovascular pharmacological action, Beijing College of Traditional Chinese Medicine's journal 1990,13 (4), 47-49].The inventor is carrying out this compound recipe in the process of systematic study, finds that this side has to resist myocardial ischemia preferably and antiarrhythmic effect, and compound recipe has been carried out the Study on extraction of effective site at these two clinical indications.
Summary of the invention:
The purpose of this invention is to provide a kind of linggui zhugan decoction extraction process and medical usage thereof, it can be used for resisting myocardial ischemia and the arrhythmia clinical indication.The present invention is achieved in that with the pharmacodynamics effect to be index, extracting method commonly used is screened, after determining the optimum extraction solvent, adopt Orthogonal Experiment and Design that extraction process is optimized, determine that linggui zhugan decoction is used to resist myocardial ischemia and the extraction process of antiarrhythmic effective site; Resist myocardial ischemia and the antiarrhythmic pharmacodynamic study with the effective site of extracting.Adopt the orthogonal test side of tearing open, the linggui zhugan decoction prescription is carried out flavour of a drug and dose is added and subtracted simultaneously, optimize this prescription, the prescription of determining divides and takes for three times for Poria 12 grams, Ramulus Cinnamomi 9 grams, the Rhizoma Atractylodis Macrocephalae 9 grams, Radix Glycyrrhizae 6 grams.
The extraction solvent that is adopted is considered industrial practical situation, water precipitation etc. behind different concentration ethanol precipitation, different concentration ethanol lixiviate and the ethanol extraction behind optional water, the decocting.Should be index with pharmacological effect, determine that 95% ethanol is the optimum extraction solvent.
After determining the optimum extraction solvent, adopt Orthogonal Experiment and Design, investigate, chosen the combination of each factor optimum level, as optimum extraction process as solvent load, extraction time, extraction time etc. to influencing the factor of extracts active ingredients efficient.With the content of 3 main pharmacodynamics compositions wherein is index, has determined that optimum extraction process is for adding 10 times to 95% ethanol of medical material amount, reflux, extract, 3 times, each 60 minutes.
In the pharmacodynamic study that linggui zhugan decoction resists myocardial ischemia, select a kind of suitable positive control drug for use, to the time-to-live of mice under the normobaric hypoxia condition, the Acute Myocardial Ischemia in Rats due to the isoproterenol, with pastille serum coronary flow and the heartbeat effect on amplitude dirty of method of serum pharmacology preparation to guinea-pig heart; Pharmacodynamic index to anesthetized dog acute myocardial ischemia and myocardial infarction and hemodynamic influence is studied.
In linggui zhugan decoction antiarrhythmic pharmacodynamic study, select a kind of suitable positive control drug for use, chloroform is caused the pharmacodynamic indexs such as influence that quiver in the mice chamber, barium chloride brings out rat ventricular, epinephrine brings out the rabbit tachyarrhythmia study.
Advantage of the present invention has provided a kind of new linggui zhugan decoction extraction process, be expected resist myocardial ischemia and the medicine of arrhythmia clinical indication in use.
Description of drawings:
Fig. 1 determines optimum extraction solvent table as a result for selecting the arrhythmia test
The specific embodiment:
Embodiment:
1, select arrhythmia experiment (the antagonism chloroform causes the mice chamber and quivers) that 11 kinds of extracting method have been carried out primary dcreening operation, select wherein 5 kinds of methods, the experiment (the antagonism isoproterenol causes the rat heart muscle ischemia) of selecting again to resist myocardial ischemia is carried out multiple sieve to 5 kinds of methods selecting, determine the optimum extraction solvent, the results are shown in Figure 1.
2, extraction process optimization: select L for use
9(3)
4Orthogonal table,, is investigated as extracting quantity of solvent, extraction time, extraction time etc. influencing the factor of extraction efficiency for carrying mark with three active constituent contents in the side and yield of extract, has determined optimised process.
3, the drug effect result that resists myocardial ischemia shows: linggui zhugan decoction can obviously prolong the time-to-live of mice under the normobaric hypoxia condition; Acute Myocardial Ischemia in Rats due to the alleviation isoproterenol, superoxide dismutase (SOD) activity of enhancing ischemic myocardium reduces myocardium malonaldehyde (MDA) content; Pastille serum with the method for serum pharmacology preparation can significantly increase dirty coronary flow of guinea-pig heart and heartbeat amplitude; Obviously improve dog acute myocardial ischemia and myocardial infarction, reduce through the shown infarct of N-BT dyeing; Can significantly increase the coronary flow of anesthetized dog, reduce coronary resistance, improve the blood supply oxygen supply of cardiac muscle; Reduce heart rate, LVP, dp/dtmax, coefficient of oxygen utilization, oxygen consumption index, thereby reduce myocardial oxygen consumption; Reduce BSP, LVEDP, reduce cardiac preload, increase heart blood perfusion flow etc.
4, the arrhythmia experimental result shows: linggui zhugan decoction quivers to chloroform induced mice chamber obvious protective effect; Can obviously shorten the barium chloride proarrhythmia time, reduce the incidence rate of two-phase arrhythmia; But the significant prolongation epinephrine causes the rabbit arrhythmia time started, shortens arrhythmia and holds time.
Claims (6)
1, linggui zhugan decoction extraction process and medical usage thereof, it is that phlegm retention name side linggui zhugan decoction resists myocardial ischemia and the secondary of arrhythmia clinical indication begins to controlling in the Zhang Zhongjing treatise on Febrile Diseases, and it has been carried out extracting the optimization of choice of Solvent and extraction process, it is characterized in that: adopt Orthogonal Experiment and Design that extraction process is optimized, the weight ratio of Poria, Ramulus Cinnamomi, the Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae is 12: 9: 9 in the prescription: 6, adopt solvent extraction.
2, linggui zhugan decoction extraction process according to claim 1 is characterized in that: the optional water of the solvent that is adopted, different concentration ethanol, also can adopt water to put forward behind back different concentration ethanol precipitation and the ethanol extraction several different methods such as water precipitation.
3, linggui zhugan decoction extraction process according to claim 1 is characterized in that: used extraction solvent can be 10 times, 8 times, 6 times amount solvents that are equivalent to the raw medicinal herbs consumption; Extraction time can be 3 times, 2 times, 1 time; Extraction time can be 30 minutes, 60 minutes, 90 minutes.
4, linggui zhugan decoction according to claim 1, it is characterized in that: linggui zhugan decoction is to the time-to-live of mice under the normobaric hypoxia condition, the Acute Myocardial Ischemia in Rats due to the isoproterenol, pastille serum coronary flow and the heartbeat effect on amplitude dirty with the method for serum pharmacology preparation to guinea-pig heart, in the pharmacodynamic experiment to anesthetized dog acute myocardial ischemia and myocardial infarction and hemodynamic influence, its drug effect is compared no significant difference with selected positive control drug.
5, linggui zhugan decoction according to claim 1, it is characterized in that: quiver, barium chloride bring out rat ventricular, epinephrine bring out in the pharmacodynamic experiments such as influence of rabbit tachyarrhythmia in that chloroform is caused by the mice chamber for linggui zhugan decoction, and its drug effect is compared no significant difference with selected positive control drug.
6, a kind of medical usage of linggui zhugan decoction as claimed in claim 1 is characterized in that: can be used for the preparation treatment and resist myocardial ischemia and the antiarrhythmic medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02109499 CN1377688A (en) | 2002-04-22 | 2002-04-22 | Extracting process for decoction of poria, Bighead Atractylodes, cinnamom and Lirorice and its medicinal use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02109499 CN1377688A (en) | 2002-04-22 | 2002-04-22 | Extracting process for decoction of poria, Bighead Atractylodes, cinnamom and Lirorice and its medicinal use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1377688A true CN1377688A (en) | 2002-11-06 |
Family
ID=4740688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02109499 Pending CN1377688A (en) | 2002-04-22 | 2002-04-22 | Extracting process for decoction of poria, Bighead Atractylodes, cinnamom and Lirorice and its medicinal use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1377688A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283895A (en) * | 2010-06-17 | 2011-12-21 | 苏州知微堂生物科技有限公司 | Preparation technology and production method of integrated novel Lingguizhugan decoction dosage form |
| CN108355028A (en) * | 2018-05-09 | 2018-08-03 | 陈竹涛 | A kind of Chinese medicine and preparation method thereof for treating myocardial infarction |
| CN108653387A (en) * | 2018-06-26 | 2018-10-16 | 安徽中医药大学 | Linggui zhugan decoction compound granule and preparation method thereof |
| CN110464746A (en) * | 2019-08-28 | 2019-11-19 | 贵州景诚制药有限公司 | A kind of sweet pharmaceutical composition of Siberian cocklebur osmanthus art, preparation method and detection method |
| CN110772561A (en) * | 2019-11-11 | 2020-02-11 | 健民药业集团股份有限公司 | Traditional Chinese medicine with diuretic function |
| CN112336765A (en) * | 2020-11-24 | 2021-02-09 | 湖南新汇制药股份有限公司 | Preparation method of poria cocos, cassia twig, rhizoma atractylodis and licorice root soup |
-
2002
- 2002-04-22 CN CN 02109499 patent/CN1377688A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283895A (en) * | 2010-06-17 | 2011-12-21 | 苏州知微堂生物科技有限公司 | Preparation technology and production method of integrated novel Lingguizhugan decoction dosage form |
| CN108355028A (en) * | 2018-05-09 | 2018-08-03 | 陈竹涛 | A kind of Chinese medicine and preparation method thereof for treating myocardial infarction |
| CN108653387A (en) * | 2018-06-26 | 2018-10-16 | 安徽中医药大学 | Linggui zhugan decoction compound granule and preparation method thereof |
| CN110464746A (en) * | 2019-08-28 | 2019-11-19 | 贵州景诚制药有限公司 | A kind of sweet pharmaceutical composition of Siberian cocklebur osmanthus art, preparation method and detection method |
| CN110464746B (en) * | 2019-08-28 | 2021-10-08 | 贵州景诚制药有限公司 | Linggui shugan medicinal composition, preparation method and detection method |
| CN110772561A (en) * | 2019-11-11 | 2020-02-11 | 健民药业集团股份有限公司 | Traditional Chinese medicine with diuretic function |
| CN110772561B (en) * | 2019-11-11 | 2022-06-14 | 健民药业集团股份有限公司 | Traditional Chinese medicine with diuretic function |
| CN112336765A (en) * | 2020-11-24 | 2021-02-09 | 湖南新汇制药股份有限公司 | Preparation method of poria cocos, cassia twig, rhizoma atractylodis and licorice root soup |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101347612B (en) | Compound medicament for treating common cold cough and acute/chronic bronchitis, and preparation method thereof | |
| CN102512521A (en) | Traditional Chinese medicine composition for treating apoplexy sequela | |
| CN104288245B (en) | Anti-aging and constitutional pharmaceutical composition and preparation method thereof and detection method | |
| CN101317964B (en) | Chinese medicinal composition with spleen-enlivening and kidney-tonifying function, preparation and uses thereof | |
| CN102145089B (en) | Traditional Chinese medicine for treating heart failure | |
| CN1377688A (en) | Extracting process for decoction of poria, Bighead Atractylodes, cinnamom and Lirorice and its medicinal use | |
| CN104147345B (en) | A kind of Chinese medicine composition for treating cardiovascular autonomic neuropathy | |
| CN101181418B (en) | Medicine composition for curing arrhythmia and preparation method and detection method thereof | |
| CN103463425B (en) | A kind of treat blood-stasis internal-depression Chinese medicine extract and preparation and method for making | |
| CN102397460B (en) | Preparation method of Kang'erxin capsule | |
| CN102526676B (en) | Traditional Chinese medicine for treating chronic heart failure, preparation method and administration mode | |
| CN102357134B (en) | Medicine for preventing and treating Alzheimer disease and cerebral thrombosis and preparation method thereof | |
| CN103316074B (en) | Medicine composite of halenia corni extract, astragalus extract and liquorice extract as well as preparation and application of medicine composite | |
| CN104042895A (en) | Traditional Chinese medicine composition for treating systemic lupus erythematosus and use thereof | |
| CN100479837C (en) | Weinakang for treating ischemic cerebrovascular disease and senile dementia and preparation method thereof | |
| CN1150839C (en) | Health food with blood pressure and blood lipoid regulating function and its production process | |
| CN104740054A (en) | Pharmaceutical composition for treating myocardial ischemia as well as preparation method and use of pharmaceutical composition | |
| CN107648565A (en) | A kind of pharmaceutical composition for treating depression and preparation method thereof | |
| CN108186821A (en) | A kind of Chinese medicine composition for treating nervous headache and its preparation method and application | |
| CN104173681B (en) | Traditional Chinese herbal medicine composition for treating castration-resistant prostate cancer | |
| CN1994426B (en) | Preparation method, and identification and assaying method of 'Sheng Mai' soft capsule | |
| CN103800698B (en) | Fibrotic pharmaceutical composition of a kind for the treatment of organs and its production and use | |
| CN108066494A (en) | A kind of enuresis nocturna frequency effect liquid and preparation method thereof | |
| CN101352562A (en) | Chinese medicine preparation for treating cardiovascular and cerebrovascular diseases and method for preparing the same | |
| CN100493535C (en) | Compounding traditional Chinese medicine-Xueyaping for treating hypertension, and its preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |