CN1377586A - Sobering-up and drunkenness-preventing liver protective beverage containing dehydrogenase - Google Patents
Sobering-up and drunkenness-preventing liver protective beverage containing dehydrogenase Download PDFInfo
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- CN1377586A CN1377586A CN 01141609 CN01141609A CN1377586A CN 1377586 A CN1377586 A CN 1377586A CN 01141609 CN01141609 CN 01141609 CN 01141609 A CN01141609 A CN 01141609A CN 1377586 A CN1377586 A CN 1377586A
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Abstract
The present invention provides a beverage containing dehydrogenase, named sobering-up syrup, for serving while drinking. It protects liver by making alcohol decompose in stomach, rather than in liver. Technologically, the present invention features that a kind of lactic acid bacteria with strong activity of generating dehydrogenase is used to prepare sour milk, nectar or oral liquor, which is taken during drinking so as to form protecting mucous membrane to prevent alcohol from entering fast into blood and liver, to make alcohol degrade partially inside stomach under the action of dehydrogenase and to protect liver and prevent drunkenness.
Description
The present invention proposes a kind of novel relieving alcoholism and preventing drunkenness beverage and manufacture method thereof.More particularly, the present invention proposes a kind ofly to take beverage or the tablet that contains alcohol dehydrogenase simultaneously drinking, earlier the drunkenness-preventing liver protective method by the liver decomposing alcohol in stomach and not.
Drinking is a kind of enjoyment in human daily life and the doings.Yet excessive consumption of alcohol not only influences health, but also can influence social normal order.Therefore, drunk and excessive drinking has become the common social effects of pollution in various countries, the world today, and pre-preventing drunkenness is the public good major issue that becomes protection health and public safety.
Drunkly be actually a kind of alcohol (ethanol) intoxicating phenomenon, because back 90% alcohol of drinking is decomposed by liver, and alcohol has certain toxicity to liver cell.Though responsible drinking can be inspired enthusiasm, frequent and excessive consumption of alcohol must increase burden of liver, even the infringement liver function causes and diseases such as alcoholic hepatitis, fatty liver and cirrhosis causes the irreversible damage of liver function, so that can not give treatment to.Long-term drunk reproductive system endocrine disturbance and the fecundity decline that also can cause influences descendants's genetic constitution.Therefore, pre-preventing drunkenness is not only current public and social interest major issue, and is the good thing of benefiting the descendants, will be understood that anti-intoxication than the more social urgent need of abstinence from alcohol.
Anti-intoxication best approach is that the wine that drink is gone into decomposes rapidly in stomach and in the blood earlier, reduces alcohol as far as possible and enters blood circulation system and go into liver, to alleviate burden of liver.Alcohol content reduces and can drink and not liquor-saturated in the blood, prevents that alcohol from entering liver in a large number and can protect liver, thereby plays sober-up and anti-drunk and protect the liver the comprehensive effect of body-building.How just can realize this goal, its key problem in technology is how to impel alcohol to obtain biodegradation earlier in stomach, this mentality of designing of the present invention just.
Alcohology is called ethanol.After drinking, a large amount of ethanol enter blood circulation system through the stomach absorption and cause flushed symptom immediately, cause alcoholism (drunk) symptoms such as dizziness, vomiting then.The ethanol major part that enters blood is degraded to acetaldehyde and acetate through dehydrogenase in liver, be decomposed into carbon dioxide and water at last.Someone drinks not liquor-saturated is that the alcohol energy quilt that drink is gone into is enzymolysis rapidly, promptly alcohol-free poisoning symptom because the interior dehydrogenase activity of its body is strong.But this kind enzymatic activity is not strong in most of people (particularly Asia yellow) body, thereby is unequal to capacity for liquor.To be that stomach is dirty do not produce dehydrogenase thereby there is no the decomposing alcohol ability key of problem, and the alcohol that drink is gone into has only by the gastro-intestinal digestion road and absorbs, and could decompose under the effect of dehydrogenase after entering liver.The dehydrogenase activity that most of people's livers produce is not strong, and excess ethyl alcohol enters with blood can not in time decompose and even make the liver cell poisoning to damage liver function in the liver.Therefore, reach the relieving alcoholism and protecting liver purpose, must go into to contain the first alcohol of in stomach, degrading of material ability of dehydrogenase, give up this and not have good method from external clothes.
How just can obtain external dehydrogenase and be used for relieving alcoholism and protecting liver? many folk prescriptions that relieve the effect of alcohol are arranged at all times, there are tens kinds in methods of relieving the effect of alcohol such as China's drink vinegar commonly used among the people, strong tea, milk, egg white, thin rice gruels, yet these folk prescriptions mostly are liquor-saturated back and use, or alleviation of alcohol rapid absorption in stomach and intestine arranged, actually there is no much antialcoholism functions.The nearly more than ten years, market more existing relieve the effect of alcohol medicine or health products are sold, and are oyster extract and taurine etc. as " liver-protecting and alcoholism-relieving sheet " its Main Ingredients and Appearance, it is said protection, nourish liver and the effect of prevention cirrhosis, but and the not mentioned dehydrogenase antialcoholism action that contains.Other has " clear after drinking " product, is known as efficient sober-up nutrition liquid, but is haw and Chinese herbal medicine extract in fact, there is no the dehydrogenase composition.The legend Chinese herbal medicine FI puerariae and the root of kudzu vine have antialcoholism function, but also do not contain dehydrogenase behind the drying one-tenth medicinal material, as perhaps produce effect be the antialcoholism action that activates liver.
Task of the present invention is seek to produce that the alcohol dehydrogenase amount is many, degraded ethanol is fast and enrich in the source, living resources with low cost from nature.
The various biologies of nature comprise multiple enzymes such as all containing dehydrogenase in animal and plant and the microbial body, but extract with the easiest processing of the enzyme of microorganisms.It is the source with various microorganisms all that modern industry is produced enzyme preparation product (comprising protease and amylase etc.).Because microbial reproduction is fast, can suitability for industrialized production, thereby with low cost.It is more to produce dehydrogenase with saccharomycete and some bacterium in microorganism.Through research for many years, we filter out the high bacterium of several alcohol dehydrogenase activities finally, the highest bacterium called after of the wherein a kind of dehydrogenase activity bacterium of relieving the effect of alcohol, numbering J96.
The J96 ne ar is an elongated rod-shaped, blunt garden, two ends; Size is 0.6-0.8 * 2.2-5.5 micron, and how single thalline is or become two-phase to join; The gemma ellipse, inferior end is given birth to or middle life, and sporangiocyst does not expand.The major physiological of J96 bacterium, biochemical characteristic are various saccharides can be fermented into lactic acid, and milk is solidified, aerobic or anaerobic growth, optimum growth temperature 40-50 ℃ and little acid (pH=6-7) environmental condition.Classification is identified through Institute of Microorganism, Academia Sinica, the formal name used at school of this bacterium is bacillus coagulans (Bacillus coagulans), belongs to one of " safety can direct feeding bacterial classification " of classifying that food and drug administration (FDA) and feed supervision association (AAFCO) announces as.Through Labour Hygiene ﹠ Occupational Disease Inst. of Chinese Academy of Prevention Med's check, this bacterium is nontoxic to bio-safety.The microorganism that bacillus coagulans J96 has sent China Committee for Culture Collection of Microorganisms common micro-organisms center to be used for proprietary program September 24 calendar year 2001 preserves, and is numbered CGMCC NO.0634.For ease of narration, following this paper abbreviates this bacterial strain as the J96 bacterium.
Through evidence, the J96 Pseudomonas can be converted into L (+) lactic acid (general lactic acid bacteria only transforms 50-70% sugar) that human body is easy to absorb with 90% above glucose or lactose, thereby more be good for one's health in producing lactic acid bacteria.The present invention tests discovery, is inoculated into the sour milk that can be fermented into sweet and sour taste in the pasteurized milk with the J96 bacterium, and nutritive value should be higher with commercially available yoghourt is the same for its taste.The more important thing is that through domestication breeding we find that it is that unique energy replaces the carbohydrate growth and breeding that this bacterium can utilize alcohol.Detect with general dehydrogenase activity indicator INT (iodine nitro tetrazolium chloride), the J96 lawn by the colourless redness that becomes, shows the strong active reaction of dehydrogenase in tens seconds; In order to detect the sour milk that bacterium is thus made, can in 10-20 minute, show red dehydrogenase reaction.In view of the above, we propose to see Fig. 1 with the technology path of modern biological project new technology development relieving alcoholism and preventing drunkenness product.
Mentality of designing of the present invention does not also require that the ethanol of drink being gone into from external introducing dehydrogenase all decomposes, and onlys demand part, degraded fast, both can inspire enthusiasm after drinking and don't flushed more unlikely injury hepatocellular " drink and not liquor-saturated " purpose thereby make.
Implementing this invention comprises J96 bacterial classification cultivation and fermentation and contains enzymolysis Wine different dosage form production method two large divisions; Wherein bacterial classification is cultivated and is the key problem in technology part, and the bright preparation of beverage of all the other different dosage forms is just made product by the known production technology of the public after J96 bacterial classification or zymotic fluid.1.J96 bacterial classification cultivation and fermentation
(1) bacterial classification condition of culture
Culture medium: glucose 0.3%, soy peptone 0.2%, yeast extract 0.8%, dipotassium hydrogen phosphate (K
2HPO
4) 0.1%, magnesium sulfate (MgSO
47H
2O) 0.02%, manganese sulfate (MnSO
4) 0.002%, pH nature, 112 ℃ of sterilizations in 30 minutes.Solid medium adds agar 2% in addition, and bevel was cultivated 48 hours for 35 ℃ in order to preserve bacterial classification.
(2) strain fermentation condition of culture
Culture medium: glucose 2.0%, soy peptone 0.5%, yeast extract 0.5%, K
2HPO
40.1%, MgSO
47H
2O 0.02%, CaCO
31.0%, pH nature, 112 ℃ of sterilizations in 30 minutes.
Shaken cultivation: 200 rev/mins of rotating speeds, cultivation temperature 35-40 ℃, incubation time 48 hours.
Seeding tank and fermentation tank: culture medium is the same, tank pressure 0.5kg/cm
2, throughput 0.5-1: 1 cubic metre/ton, 200 rev/mins of mixing speeds, 35 ℃ of temperature (the fermented and cultured temperature is 42 ℃ fast).
The zymotechnique flow process is seen Fig. 2.
Bacterium count content detection routinely bacteriology plate count method carry out, alcohol dehydrogenase activity is as with fast detection method being dropping INT indicator, the enzymatic activity quantitative analysis is by public's known conventional detection method (Guo Yong chief editor: enzyme engineering, China Light Industry Press, 1996).2. different dosage form alcohol-decomposing beverage production
The aforementioned technology path that contains dehydrogenase relieving alcoholism and preventing drunkenness beverage that proposes according to the present invention can be made into following different dosage form relieving alcoholism and preventing drunkenness product, and existing explanation in conjunction with the embodiments: embodiment 1
Sour-milk type relieving alcoholism and preventing drunkenness beverage: replace common lactic acid bacteria culturers to make sour milk with the J96 bacterial classification, the taste of existing commercially available yoghourt has the function that dehydrogenase relieves the effect of alcohol again, regulates intestinal beneficial bacterium group's microecologic regulator effect simultaneously in addition.Present sour milk brand in all parts of the country is a lot, and the sour milk food of sweet and sour taste is thus much a kind of again but have relieving alcoholism and preventing drunkenness and health care simultaneously.
The characteristics of sour-milk type alcohol-decomposing beverage also are after people drink, earlier be furnished with the milk that one deck contains dehydrogenase at the coat of the stomach mucomembranous surface, promptly may making wherein after drinking, alcohol can not entered blood circulation system by the stomach absorption rapidly, both created condition, and produced intoxicating phenomenon and play and protect the liver anti-drunk function thereby avoided alcohol to enter liver in a large number again for dehydrogenase degraded alcohol in the sour milk.
Because this type of new high-tech product function uniqueness has been different from general in the market sour milk, thereby " dew relieves the effect of alcohol " new trade name by name.
Embodiment 2
Fruit tea type alcohol-decomposing beverage: add dextrin with J96 strain fermentating liquid and multiple fruit juice (each 50%) and make the fruit tea beverage.The effect that adds dextrin is to protect gastric mucosa, prevents that alcohol from being absorbed by stomach rapidly and is dehydrogenase degraded in the fruit tea, and its relieving alcoholism and preventing drunkenness function class is similar to sour milk, the selection of a last alcohol-decomposing beverage of can serving the meals.But this series products is different from the general fruit tea on the market again, " fruit tea of sobering up " by name.
Embodiment 3
The fruit juice type alcohol-decomposing beverage: being mixed into J96 strain fermentating liquid and fruit juice (50%) and containing dehydrogenase fruit juice, is tasty drink on the dining table, and the sober-up and anti-drunk function is arranged again.
Embodiment 4
Capsule/tablet form " Sobering up extraction ": will dehydrate into powder by the sour milk that inoculation J96 bacterium is made and incapsulate or add soluble starch and be pressed into tablet, and take in 1-2 hour before drinking.Dehydrogenase activation ratio liquid dosage form product is slow in this type of dry powder or the tablet, take before therefore will drinking, but this series products holding time is long, and being easy to carry and taking is a big advantage.
Embodiment 5
By implementing the sour milk that 1 described method inoculation J96 bacterium is made in the milk, by known enzyme preparation method of purification (Guo Yong, the enzyme engineering of the public; China Agricultural University's microbiology experiment instruction: experiment nine, dehydrogenase is purified), behind alcohol dehydrogenase extraction purifying in the sour milk, make oral liquid, also be convenient to preserve and carry and take.
Claims (5)
1. one kind contains dehydrogenase Antialcoholic liver-protecting sour milk, it is characterized in that it utilizes special product lactic acid bacteria--bacillus coagulans (patent bacterium numbering CGMCC 0634), milk can be made yogurt and contain alcohol dehydrogenase, because of sour milk can slow down gastric mucosa absorption alcohol enzymolysis is arranged again, thereby the Antialcoholic liver-protecting function arranged, special name be " dew relieves the effect of alcohol ".
2. according to the described enzymolysis vinic acid milk of containing of claim 1, it is characterized in that sour milk dehydrated and make capsule or be pressed into tablet, be convenient to preserve and carry.
3. according to the described enzymolysis vinic acid milk that contains of claim 1, wherein alcohol dehydrogenase is made oral liquid after purifying, and being convenient to take is its feature.
4. one kind contains the dehydrogenase alcohol-dissolving liver-protecting drink, it is characterized in that it contains bacillus coagulans (patent bacterium numbering CGMCC 0634) zymotic fluid with addition of various fruit juice, is tasty drink, contains the dehydrogenation antialcoholism function again.
5. according to the described enzymolysis Wine that contains of claim 4, it is characterized in that wherein also making thickness fruit tea type beverage, both contained alcohol dehydrogenase, can slow down gastric mucosa again and absorb alcohol, thereby have the facilitating alcohol metabolism and protecting liver effect with addition of dextrin.
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| CN 01141609 CN1183839C (en) | 2001-09-28 | 2001-09-28 | Sobering-up and drunkenness-preventing liver protective beverage containing dehydrogenase |
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| CN 01141609 CN1183839C (en) | 2001-09-28 | 2001-09-28 | Sobering-up and drunkenness-preventing liver protective beverage containing dehydrogenase |
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| CN1377586A true CN1377586A (en) | 2002-11-06 |
| CN1183839C CN1183839C (en) | 2005-01-12 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904351A (en) * | 2010-08-10 | 2010-12-08 | 河北三元食品有限公司 | Antialcoholic sour milk and preparation method thereof |
| CN104640554A (en) * | 2012-08-03 | 2015-05-20 | 卓越人生有限责任公司 | Compositions and methods for reducing blood alcohol content |
| CN106212673A (en) * | 2016-07-23 | 2016-12-14 | 安徽乙地生态科技有限公司 | A kind of method utilizing Bacillus coagulans to prepare Yoghourt |
| CN109007676A (en) * | 2018-08-17 | 2018-12-18 | 江苏新申奥生物科技有限公司 | A kind of lactic acid bacteria freeze drying powder and preparation method thereof containing acetaldehyde dehydrogenase |
| CN111548947A (en) * | 2019-06-06 | 2020-08-18 | 郑州大学 | Lactic acid bacteria product and its sobering-up use |
| CN112385846A (en) * | 2019-08-14 | 2021-02-23 | 大江生医股份有限公司 | Application of bacillus coagulans in preparation of composition for relieving alcoholism and edible product |
-
2001
- 2001-09-28 CN CN 01141609 patent/CN1183839C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904351A (en) * | 2010-08-10 | 2010-12-08 | 河北三元食品有限公司 | Antialcoholic sour milk and preparation method thereof |
| CN101904351B (en) * | 2010-08-10 | 2012-05-23 | 河北三元食品有限公司 | Antialcoholic sour milk and preparation method thereof |
| CN104640554A (en) * | 2012-08-03 | 2015-05-20 | 卓越人生有限责任公司 | Compositions and methods for reducing blood alcohol content |
| US10918679B2 (en) | 2012-08-03 | 2021-02-16 | Life Well Lived, Llc | Compositions and methods for reducing blood alcohol content |
| US11771724B2 (en) | 2012-08-03 | 2023-10-03 | Life Well Lived, Llc | Compositions and methods for reducing blood alcohol content |
| CN106212673A (en) * | 2016-07-23 | 2016-12-14 | 安徽乙地生态科技有限公司 | A kind of method utilizing Bacillus coagulans to prepare Yoghourt |
| CN109007676A (en) * | 2018-08-17 | 2018-12-18 | 江苏新申奥生物科技有限公司 | A kind of lactic acid bacteria freeze drying powder and preparation method thereof containing acetaldehyde dehydrogenase |
| CN111548947A (en) * | 2019-06-06 | 2020-08-18 | 郑州大学 | Lactic acid bacteria product and its sobering-up use |
| CN112385846A (en) * | 2019-08-14 | 2021-02-23 | 大江生医股份有限公司 | Application of bacillus coagulans in preparation of composition for relieving alcoholism and edible product |
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|---|---|
| CN1183839C (en) | 2005-01-12 |
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