CN1374303A

CN1374303A - Production process of water soluble ginkgo leaf extractive

Info

Publication number: CN1374303A
Application number: CN 02113533
Authority: CN
Inventors: 张迪清; 何珺; 何照范; 邓秀英; 张天常; 黄英
Original assignee: GUIZHOU BIO-CHEMICAL ENGINEERING CENTER
Current assignee: GUIZHOU BIO-CHEMICAL ENGINEERING CENTER
Priority date: 2002-03-26
Filing date: 2002-03-26
Publication date: 2002-10-16

Abstract

The present invention is the production process of water soluble gingko leaf extractive. Gingko leaf is extracted with hot alcohol repeatedly to obtain concentrate, and the concentrate is treated through mixing with water, letting stand, taking supernant, processing in macroporous resin column, etc. to obtain coarse extractive. The coarse extractive is further processed through alcohol dissolution, processing in macroporous resin column, etc. to obtain water soluble gingko leaf extractive without ginkgolic acid. Compared with available technology, the present invention combines the extraction of ginkgo leaf extractive and the elimination of ginkgolic acid and has low production cost and high ginkgolic acid eliminating effect.

Description

The production technique of water soluble ginkgo leaf extract

The present invention is a kind of production technique of water soluble ginkgo leaf extract, and the particularly water-soluble production technique that does not contain the Folium Ginkgo extract of ginkgoic acid belongs to the technical field of fine chemistry industry

In the prior art, the effective constituent that the relevant technologies that adopts is extracted from Ginkgo Leaf generally all contains this class impurity of ginkgoic acid, because ginkgoic acid has toxic side effect, limited the application of Folium Ginkgo extract, prior art will be extracted effective extract and be divided into two work this Folium Ginkgo extract being carried out depickling then from Ginkgo Leaf, cause increasing production process and production cost like this, the level of prior art depickling is not high simultaneously, is difficult to thoroughly remove the ginkgoic acid in the Folium Ginkgo extract.

The objective of the invention is to: the production technique that a kind of water soluble ginkgo leaf extract is provided, this technology is extracted Folium Ginkgo extract and is directly removed ginkgoic acid in the Folium Ginkgo extract of acquisition from Ginkgo Leaf, that is to say, original two step technology are synthesized the method for once just dealing with problems, this explained hereafter is with low cost, deacidification effect is good, ginkgoic acid remains in below the lppm, the product good water solubility, dissolving, limpid transparent in cold water, enlarged the Ginkgo Leaf Application Areas, the application of particularly medical injection product.

The present invention constitutes like this: Ginkgo Leaf is heated lixiviate several times repeatedly with solution, soaking the liquid concentration that body obtains several times, concentrated solution leaves standstill after adding water, get supernatant liquor then and cross adsorption column, collected the effluent liquid behind the post, utilize prior art to handle then, concentrate, dry, obtain ginkgo biloba crude extract, dissolve these ginkgo biloba crude extracts with alcohol again, stir, filter, filterable then liquid is crossed adsorption column, collect the effluent liquid behind the post, and utilized prior art to handle then, concentrate, drying obtains the water-soluble Folium Ginkgo extract that does not contain ginkgoic acid, when producing, adsorption column should at first carry out plastic resin treatment according to existing method; Specifically: with Ginkgo Leaf 10-100 kilogram water or organic solvent in room temperature-75 degree centigrade, lixiviate, filter, residue lixiviate 1-2 time again, merge vat liquor, concentrate vat liquor with vacuum-evaporator unit, concentrated solution adds and is water-cooled to below 40 degrees centigrade, staticly settle more than 8 hours, centrifugal treating, get supernatant liquor then and cross adsorption column, every 2 minutes effluent liquid is carried out once saturated check, then stopped post if any saturated reaction, at this moment cross post with 300L distilled water, to effluent liquid limpid till, 200L crosses post with 50-95% alcohol, collects the effluent liquid that color is divided than the deep, behind the film under vacuum vaporizer recovered alcohol, be positioned over drying in the drying plant, obtain ginkgo biloba crude extract, join in the 1-20 kilogram gram ginkgo biloba crude extract of acquisition with 30-95% alcohol 100L again, stir, filter, and then with 30-95% alcohol 50L dissolving once, refilter, add 300L distilled water in the filtrate, stir, placed 12 hours, separate out the partly precipitated thing, use filter paper filtering, cross post, cross post with 100L distilled water again, collect the effluent liquid behind the post, and utilized prior art to handle then, concentrate, drying obtains the water-soluble Folium Ginkgo extract that does not contain ginkgoic acid; Among the present invention, the adsorption column of use is macroporous resin column, ion exchange resin column, activated carbon column, polyamide column, siliceous earth column.

Compared with prior art: this technology is extracted Folium Ginkgo extract and is directly removed ginkgoic acid the Folium Ginkgo extract of acquisition from Ginkgo Leaf, that is to say, original two step technology are synthesized the method for once just dealing with problems, this explained hereafter is with low cost, deacidification effect is good, and the product of acquisition meets the technical requirements of the countries concerned for medicine.

Embodiments of the invention 1: Ginkgo Leaf is heated lixiviate several times repeatedly with alcohol, soaking the liquid concentration that body obtains several times, concentrated solution leaves standstill after adding water, get the commercially available excessively macroporous resin column of supernatant liquor then, collected the effluent liquid behind the post, utilize prior art to handle then, concentrate, dry, obtain ginkgo biloba crude extract, dissolve these ginkgo biloba crude extracts with alcohol again, stir, filter, filterable then liquid is crossed macroporous resin column, collect the effluent liquid behind the post, and utilized prior art to handle then, concentrate, drying obtains the water-soluble Folium Ginkgo extract that does not contain ginkgoic acid, when producing, macroporous resin column should at first be carried out plastic resin treatment according to existing method;

Embodiments of the invention 2: with the alcohol 900L of 100 kilograms of usefulness 60% of Ginkgo Leaf at the water-bath internal heating to 20-70 degree centigrade, lixiviate 2 hours, stirred once in per 10 minutes, filter, residue added 60% alcohol 700L, 20-70 degree centigrade of following lixiviate 2 hours, stirred once in per 10 minutes, filter, merge vat liquor, concentrate vat liquor to 150L with vacuum-evaporator unit, concentrated solution adds below the water 500L postcooling to 40 degree centigrade, staticly settle more than 12 hours, centrifugal treating is got supernatant liquor then and is crossed the DA201 post, every 2 minutes effluent liquid is carried out once saturated check, that is: get 2 of liquid, place on the white plate, add one of acetate, add 1 in 1% ferrous sulfate, as solution is mazarine or black, then post is saturated, as nondiscoloration, and post unsaturation then; Then stopped post if any saturated reaction, at this moment cross post with 400L distilled water, to effluent liquid limpid till, cross post with 80% alcohol 200L, collect the effluent liquid that color is divided than the deep, behind the film under vacuum vaporizer recovered alcohol, be positioned in the drying plant dry, obtain ginkgo biloba crude extract, join in the 10 gram ginkgo biloba crude extracts of acquisition with 95% alcohol 100L again, stir, filter, and then with 95% alcohol 50L dissolving once, refilter, add 300L distilled water in the filtrate, stir, placed 12 hours, and separated out the partly precipitated thing, use filter paper filtering, cross the DA201 post, cross post with 100L distilled water again, collected the effluent liquid behind the post, utilize prior art to handle then, concentrate, drying obtains the water-soluble Folium Ginkgo extract that does not contain ginkgoic acid.

In an embodiment of the present invention, the adsorption column of use can be macroporous resin column, ion exchange resin column, activated carbon column, polyamide column, siliceous earth column; To Ginkgo Leaf can water or organic solvent in room temperature-75 degree centigrade, lixiviate, filtration.

Claims

1, a kind of production technique of water soluble ginkgo leaf extract, it is characterized in that: Ginkgo Leaf is heated lixiviate several times repeatedly with solution, soaking the liquid concentration that body obtains several times, concentrated solution leaves standstill after adding water, get supernatant liquor then and cross adsorption column, collected the effluent liquid behind the post, utilize prior art to handle then, concentrate, dry, obtain ginkgo biloba crude extract, dissolve these ginkgo biloba crude extracts with alcohol again, stir, filter, filterable then liquid is crossed adsorption column, collects the effluent liquid behind the post, utilize prior art to handle then, concentrate, dry, obtain the water-soluble Folium Ginkgo extract that does not contain ginkgoic acid, when producing, adsorption column should at first carry out plastic resin treatment according to existing method.

2, production technique according to the described this water soluble ginkgo leaf extract of claim 1, it is characterized in that: with Ginkgo Leaf 10-100 kilogram water or organic solvent in room temperature-75 degree centigrade, lixiviate, filter, residue lixiviate 1-2 time again, merge vat liquor, concentrate vat liquor with vacuum-evaporator unit, concentrated solution adds and is water-cooled to below 40 degrees centigrade, staticly settle more than 8 hours, centrifugal treating, get supernatant liquor then and cross adsorption column, every 2 minutes effluent liquid is carried out once saturated check, then stopped post if any saturated reaction, at this moment cross post with 300L distilled water, to effluent liquid limpid till, 200L crosses post with 50-95% alcohol, collect the effluent liquid that color is divided than the deep, behind the film under vacuum vaporizer recovered alcohol, be positioned in the drying plant dry, obtain ginkgo biloba crude extract, join in the 1-20 kilogram gram ginkgo biloba crude extract of acquisition with 30-95% alcohol 100L again, stir, filter, and then with 30-95% alcohol 50L dissolving once, refilter, add 300L distilled water in the filtrate, stir, placed 12 hours, and separated out the partly precipitated thing, use filter paper filtering, cross post, cross post with 100L distilled water again, collected the effluent liquid behind the post, utilize prior art to handle then, concentrate, drying obtains the water-soluble Folium Ginkgo extract that does not contain ginkgoic acid.

3, according to the production technique of claim 1,2 described this water soluble ginkgo leaf extracts, it is characterized in that: the adsorption column of use is macroporous resin column, ion exchange resin column, activated carbon column, polyamide column, siliceous earth column.