CN1360210A - Method for using nucleic acid in antiforge purpose by hybridization technique - Google Patents
Method for using nucleic acid in antiforge purpose by hybridization technique Download PDFInfo
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- CN1360210A CN1360210A CN 00127924 CN00127924A CN1360210A CN 1360210 A CN1360210 A CN 1360210A CN 00127924 CN00127924 CN 00127924 CN 00127924 A CN00127924 A CN 00127924A CN 1360210 A CN1360210 A CN 1360210A
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Abstract
An antiforge method is characterized by that the nucleic acid is used to prepare antiforge marker and the nucleic acid molecular hybridization technique is used to check said antiforge marker. The process for preparing said antiforge marker and the application of said antiforge method are disclosed also.
Description
The present invention discloses a kind of making nucleic acid molecular hybridization technology of utilizing nucleic acid is applied to false proof method, belongs to biological field of anti-counterfeit technology.
Anti-counterfeiting technology commonly used comprises: antiforge laser holographic, magnetic anti-counterfeit, bar code are false proof, fluorescence falsification preventing, concealed anti-false, nuclear concealed anti-false, plasma concealed anti-false, ultraviolet light is false proof, coding is false proof, temperature causes that to become false proof, computer code false proof or the like.
Traditional anti-fake mark is the goods of physics or chemical property mostly, as optic metachromatic film, image scrambling, thermal change printing ink, magnetic stripe, software etc.
Begin in the world at present with biotechnology be used in false proof on, formed various biological anti-counterfeiting technology and corresponding biological anti-fake mark.These anti-counterfeiting technologies mainly adopt the antigen-antibody reaction principle, and are more accurate, sensitive than general anti-counterfeiting technology.Because antigen-antibody is protein, less stable, inactivation easily in hot environment especially, thus reduce false proof sensitivity and reliability.In addition, antigen-antibody reaction is with low uncertainty, in case know wherein a kind of composition, just easily by imitated.
An object of the present invention is to overcome underlined easily, design nucleic acid cryptanalysis technology is applied to false proof method by imitated defective in the false proof technical field of biology.
Another object of the present invention provides a kind of new anti-fake mark and detects the method for its true and false.
The invention provides a kind of new anti-counterfeiting technology and anti-fake mark, its core is a biomacromolecule---nucleic acid.The basic genetic material (gene) of all life all is a nucleic acid, and just ribodesose RNA (ribonucleic acid) (DNA) or RNA (ribonucleic acid) (RNA) nucleic acid are to be rearranged alternately by four kinds of bases, the difference of its permutation and combination, the diversity of life style on the formation earth.Article one, the DNA chain that contains 1000 base-pairs (1kb) just has 10
603Plant permutation and combination method.Present natural biological species (animals and plants and microorganism) is more than 1,000,000 kinds, can utilize therefore that to make false proof natural biological gene innumerable.The length of each species gene level is general all greater than 10
4To 10
6Kb.If set and make anti-fake mark with the nucleic acid of 1kb length, then alternative nucleic acid password just has 10
5* (10
4--10
6)=10
10--10
12More than.Therefore comprised in the nucleic acid magnanimity information provide inexhaustible source for anti-counterfeiting technology.Utilize the huge like this quantity of information of nucleic acid keying sequence can have the password of counting in hundreds of millions to can be used for anti-fake mark.
The molecular hybridization of nucleic acid is one of most widely used technology in present biological chemistry and the molecular biology research, is qualitative or the strong instrument of special RNA of detection by quantitative or dna sequencing fragment.It utilizes the base complementrity principle of nucleic acid molecules to grow up.Heating or add under the condition such as denaturant in alkaline environment, the hydrogen bond between the double-stranded DNA destroyed (sex change), two strands is separated and is split into two strands.At this moment add DNA or the RNA (strand) and the insulation (renaturation) under certain ionic strength and temperature of allos,, then when renaturation, can form the nucleic acid molecules of hybridization if there is complementary base sequence in some zone between allogeneic dna sequence DNA or the RNA.
During the conducting molecule hybridization technique, known dna or the RNA sequence fragment used as testing tool are called hybridization probe.Single stranded DNA or RNA molecular probe are only hybridized with the DNA chain of its homology complementation.When in certain anti-fake mark, adding certain specific dna fragmentation, in debating pseudoprocess, DNA is eluted from anti-fake mark, survey check with this kind dna probe and can debate out the true and false.
The present invention be will screening one section nucleic acid (can be DNA, or RNA), with a certain amount of (can be less to 10
-8Gram) is fixed on the solid phase carrier.Solid phase carrier can be various materials; as various natural or paper or film, porous particle or powder that regenerated fiber is made, natural or synthetic leather, plastics or various organic or inorganic polymer and resin, paraffin wax, natural or synthetic dead matter are as pottery, glass, crystal, metal, zeyssatite etc.Various printing ink and coating also can be used as the carrier of false proof nucleic acid.
In order to reach on the protection solid phase carrier as the purpose of the nucleic acid of anti-fake mark, can add protective seam at the surface of solid phase carriers that contains nucleic acid, for example available plastic sheeting, trehaloses etc. are made protective seam.This nucleic acid anti-fake mark can be fixed on commodity or the object or on its packing material and the adjunct.
When the needs check is told truth from falsehood, anti-fake mark of the present invention is thrown off protective seam, dissolve the nucleic acid that is fixed on the solid phase carrier with damping fluid.The kind of nucleic acid anti-fake mark is surveyed with regard to available specific making nucleic acid molecular hybridization on known detection commodity of testing staff or the article.
According to the comparison of results of hybridization and known nucleic acid probe, can judge the true and false of anti-fake mark.If can demonstrate hybridization signal, think that then anti-fake mark is true.Otherwise be false.
Utilize nucleic acid to make anti-fake mark following advantage arranged:
1. can not copying property: at first the nucleic acid password be colourless, and naked eyes are difficult to see that secondly, password is from the huge gene pool of nature, even know the password of gene but do not know to be any, the counterfeiter also can't decode and copy.And concerning the reviewer, as long as test with the analytical approach of special use, the true and false then comes into plain view.
2. reliability: have only by the probe or the PCR primer of predetermined nucleic acid encryption design and test, just might detect the existence of password and the kind of password, so the nucleic acid password has high selectivity.
3. diversity: can be easy to design ten million kind of nucleic acid password as anti-fake mark, every kind all has self corresponding probe and primer, not general mutually.So the nucleic acid password can be diversified commodity and object provides unique anti-fake mark system respectively.
4. stability: nucleic acid (particularly DNA) is stable, and particularly Gan Zao DNA deposits prolongedly, is difficult for decomposition, is suitable for the false proof of some long preservation objects.
5. extensive applicability: the nucleic acid password only needs denier (10
-8-10
-10Gram) is present in commodity or the object and can detects, therefore be applicable to commodity and the object that kind is valuable.As scientific instrument and household electrical appliance; High-grade clothing, furniture and style, entertainment article; Senior tobacco and wine, food and nutriment; Rare or the important seed of agricultural and gardening; Important certificate and file; Rare cultural relics through identifying and artwork or the like, all applicable on nearly all solid-state object.
The invention provides accompanying drawing a: Fig. 1 and be the making nucleic acid molecular hybridization method check DNA anti-fake mark true and false, among the figure, A is blank (no DNA); B is other non-specific DNAs, 10 micrograms; C is the CAT encoding gene, 0.02 microgram.
Just utilize the making nucleic acid molecular hybridization technology that nucleic acid is applied to one of false proof proposition below in conjunction with accompanying drawing
Embodiment.
Embodiment
Whether anti-fake mark is reliably single-minded, depends on to debate the dummy check method accurately.Present embodiment adopts the making nucleic acid molecular hybridization method.DNA is two nucleotide chains that move towards to be coiled on the contrary mutually together, is double-spiral structure.Article two, the hydrogen bond that relies between the nucleotide chain on adenine (A) and thymine (T) and guanine (G) and four kinds of bases of cytimidine (C) maintains.Therefore, the combination between DNA-DNA or the DNA-RNA chain promptly so-called " molecular hyridization ", its stability depends on the complementarity of the strictness between the double-stranded base fully.Be A and T complementation, G and C complementation.Single stranded DNA or RNA molecular probe are only hybridized with the DNA chain of its homology complementation.When in certain anti-fake mark, adding certain specific dna fragmentation, in debating pseudoprocess, DNA is eluted from anti-fake mark, survey the true and false with this kind dna probe and can debate out the true and false.Its concrete operations step is as follows:
1, sample preparation:, concentrate the back point on the nitrocellulose film or nylon membrane of hybridization usefulness from anti-fake mark eluted dna (about 0.02 microgram);
2, sex change, neutralization: the DNA on the film is neutralizing with the 1mol/L damping fluid through the sex change of 0.5mol/L NaOH:
3, solid-phase hybridization: place hybridization solution through the DNA of sex change film, add nucleic acid probe (isotope probe or heterotope chemiluminescence probe), in 55 ℃ of hybridization solutions 4 hours or spend the night, remove the probe of non-specific bond again through washing, use the exograph exposure at last;
4, identify: exograph contains default false proof DNA as existing the development spot shown in Fig. 1 (C), promptly proving after developing in the sample on the position of hybond membrane point sample, if as Fig. 1 (A spot do not occur shown in B), proves that then it is puppet product or the fakement of palming off.
Needing the present embodiment of explanation is a general rule, is not the running program of fixing.In the practical operation, often change to some extent, otherwise can not reach expected results with different on dna profiling and the design of primers.The operating conditions of this strictness, has no way of breaking a code because of the anti-person of emitting does not know reaction conditions for false proof very favourable again.
Claims (5)
1. one kind is utilized the making nucleic acid molecular hybridization technology that nucleic acid is applied to false proof method, it is characterized in that nucleic acid or its segment are fixed on and are used as anti-fake mark on the solid phase carrier, utilizes the making nucleic acid molecular hybridization technical checking true and false.
2. anti-fake mark as claimed in claim 1 is characterized in that described anti-fake mark can directly be fixed on any solid-state object.
3. anti-fake mark as claimed in claim 1 is characterized in that described anti-fake mark can add protective seams such as covering plastic sheeting or trehalose.
4. solid phase carrier as claimed in claim 1 is characterized in that described solid phase carrier can be natural or the solid-state material of organic or inorganic such as regenerated fiber, paper, film, leather, plastics, porous particle, powder, resin, paraffin wax, pottery, glass.
5. the method for the checking security mark true and false as claimed in claim 1 is characterized in that can judging the true and false of anti-fake mark according to the molecular hyridization assay and the known nucleic acid probe result's of nucleic acid on the anti-fake mark comparison.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00127924 CN1360210A (en) | 2000-12-18 | 2000-12-18 | Method for using nucleic acid in antiforge purpose by hybridization technique |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00127924 CN1360210A (en) | 2000-12-18 | 2000-12-18 | Method for using nucleic acid in antiforge purpose by hybridization technique |
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| CN1360210A true CN1360210A (en) | 2002-07-24 |
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|---|---|---|---|
| CN 00127924 Pending CN1360210A (en) | 2000-12-18 | 2000-12-18 | Method for using nucleic acid in antiforge purpose by hybridization technique |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020109432A1 (en) * | 2018-11-27 | 2020-06-04 | Crime Solutions Limited | Security markers |
-
2000
- 2000-12-18 CN CN 00127924 patent/CN1360210A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020109432A1 (en) * | 2018-11-27 | 2020-06-04 | Crime Solutions Limited | Security markers |
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