CN1344807A - Method of applying nucleic acid sequencing analysis technology in anti-fake - Google Patents
Method of applying nucleic acid sequencing analysis technology in anti-fake Download PDFInfo
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- CN1344807A CN1344807A CN 00125492 CN00125492A CN1344807A CN 1344807 A CN1344807 A CN 1344807A CN 00125492 CN00125492 CN 00125492 CN 00125492 A CN00125492 A CN 00125492A CN 1344807 A CN1344807 A CN 1344807A
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Abstract
The present invention discloses one method of applying nucleic acid to anti-fake. Nucleic acid is used to produce anti-fake mark and nucleic acid sequencing analysis technology is used for testing truth or false. The present invention also discloses the technological process of producing anti-fake mark and the use of the process.
Description
The present invention discloses a kind of nucleic acid sequencing analytical technology of utilizing nucleic acid is applied to false proof method, belongs to biological field of anti-counterfeit technology.
Anti-counterfeiting technology commonly used comprises: antiforge laser holographic, magnetic anti-counterfeit, barcode are false proof, fluorescence falsification preventing, concealed anti-false, nuclear concealed anti-false, plasma concealed anti-false, UV-light is false proof, coding is false proof, temperature causes that to become false proof, computer code false proof or the like.
Traditional anti-fake mark is the goods of physics or chemical property mostly, as optic metachromatic film, image scrambling, thermal change printing ink, magnetic stripe, software etc.
Begin in the world at present with biotechnology be used in false proof on, formed various biological anti-counterfeiting technology and corresponding biological anti-fake mark.These anti-counterfeiting technologies mainly adopt the antigen antibody reaction principle, and are more accurate, sensitive than general anti-counterfeiting technology.Because antigen-antibody is protein, less stable, inactivation easily in hot environment especially, thus reduce false proof sensitivity and reliability.In addition, antigen antibody reaction is with low uncertainty, in case know wherein a kind of composition, just easily by imitated.
An object of the present invention is to overcome underlined easily, design nucleic acid cryptanalysis technology is applied to false proof method by imitated defective in the false proof technical field of biology.
Another object of the present invention provides a kind of new anti-fake mark and detects the method for its true and false.
The invention provides a kind of new anti-counterfeiting technology and anti-fake mark, its core is biomacromolecule one-nucleic acid.The basic genetic material (gene) of all life all is a nucleic acid, and just thymus nucleic acid (DNA) or Yeast Nucleic Acid (RNA) nucleic acid are to be rearranged alternately by four kinds of bases, the difference of its permutation and combination, the diversity of life style on the formation earth.Article one, the DNA chain that contains 1000 base pairs (1kb) just has 10
603Plant permutation and combination method.Present natural biological species (animals and plants and microorganism) is more than 1,000,000 kinds, can utilize therefore that to make false proof natural biological gene innumerable.The length of each species gene level is general all greater than 10
4To 10
6Kb.If set and make anti-fake mark with the nucleic acid of 1kb length, then alternative nucleic acid password just has 10
5* (10
4--10
6)=10
10--10
12More than.Therefore comprised in the nucleic acid magnanimity information provide inexhaustible source for anti-counterfeiting technology.Utilize the huge like this quantity of information of nucleic acid keying sequence can have the password of counting in hundreds of millions to can be used for anti-fake mark.
DNA is the double-spiral structure that is formed by four kinds of thymus nucleic acids, and four kinds of thymus nucleic acids are divided into VITAMIN B4 (A), guanine (G), cytosine(Cyt) (C), thymus pyrimidine (T) according to its bonded base difference.Different dna sequence dnas, its based composition difference so the based composition of DNA has specificity, can be formed the specificity of judging it by the Nucleotide of measuring the section of DNA sequence.
For the dna sequence dna of trace, can be increased by the polymerase chain reaction earlier, make DNA reach sequenator and detect the effective concentration that requires.(polymerase chainreaction is to be template with the section of DNA sequence PCR), is primer with two sections oligonucleotide, at the DNA section of specific amplification under the catalysis of archaeal dna polymerase between two sections primers in the polymerase chain reaction.
The present invention be will screening one section nucleic acid (can be DNA, or RNA), with a certain amount of (can be less to 10
-8Gram) is fixed on the solid phase carrier.Solid phase carrier can be various materials; as various natural or paper or film, porous particle or powder that regenerated fiber is made, natural or synthetic leather, plastics or various organic or inorganic polymer and resin, Paraffin Wax Semi Refined, natural or synthetic inorganic substance are as pottery, glass, crystal, metal, diatomite etc.Various printing ink and coating also can be used as the carrier of false proof nucleic acid.
In order to reach on the protection solid phase carrier as the purpose of the nucleic acid of anti-fake mark, can add protective layer at the surface of solid phase carriers that contains nucleic acid, for example available plastics film, trehaloses etc. are made protective layer.This nucleic acid anti-fake mark can be fixed on commodity or the object or on its packing material and the appurtenant.
When the needs check is told truth from falsehood, anti-fake mark of the present invention is thrown off protective layer, dissolve the nucleic acid that is fixed on the solid phase carrier with damping fluid.The testing staff carries out sequential detection with method for nucleic acid sequencing to the nucleic acid on the anti-fake mark, contrasts the information nucleic acid of known nucleic acid anti-fake mark
According to the comparison of sequencing result and known nucleic acid sequence, can judge the true and false of anti-fake mark.If both unanimities, or the result is discrepant is no more than 5 polynucleotide, thinks that then anti-fake mark is true.Otherwise be false.
Utilize nucleic acid to make anti-fake mark following advantage arranged:
1. can not copying property: at first the nucleic acid password be colourless, and naked eyes are difficult to see that secondly, password is from the huge gene pool of nature, even know the password of gene but do not know to be any, the counterfeiter also can't decode and copy.And concerning the reviewer, as long as test with the analytical procedure of special use, the true and false then comes into plain view.
2. reliability: have only by the probe or the PCR primer of predetermined nucleic acid encryption design and test, just might detect the existence of password and the kind of password, so the nucleic acid password has high specificity.
3. diversity: can be easy to design ten million kind of nucleic acid password as anti-fake mark, every kind all has self corresponding probe and primer, not general mutually.So the nucleic acid password can be diversified commodity and object provides unique anti-fake mark system respectively.
4. stability: nucleic acid (particularly DNA) is stable, and particularly exsiccant DNA deposits prolongedly, is difficult for decomposing, and is suitable for the false proof of some prolonged preservation objects.
5. extensive applicability: the nucleic acid password only needs denier (10
-8-10
-10Gram) is present in commodity or the object and can detects, therefore be applicable to commodity and the object that kind is valuable.As scientific instrument and household electrical appliance; High-grade clothing, furniture and style, entertainment article; Senior tobacco and wine, food and nutritious prod; Rare or the important seed of agricultural and gardening; Important certificate and file; Rare cultural relics through identifying and artwork or the like, all applicable on nearly all solid-state object.
The invention provides an accompanying drawing: the nucleic acid encrypted message that Fig. 1 is obtained for the nucleic acid sequencing analytical technology, wherein, AGCT represents respectively: VITAMIN B4 (A), guanine (G), cytosine(Cyt) (C), thymus pyrimidine (T).
Just utilize the nucleic acid sequencing analytical technology that nucleic acid is applied to one of false proof proposition below in conjunction with accompanying drawing
Embodiment.
Embodiment
Present embodiment adopts the specificity of the method validation dna fragmentation of nucleic acid molecule order-checking.Carry out according to the explanation of the Big-Dye Terminator of PE company sequencing kit, the concrete operations step is as follows:
1. add 4 μ l reaction buffers successively, the 200ng dna profiling, the 1.6pmol special primer,
Adding distilled water makes cumulative volume reach 10 μ l.
By 96 ℃ 10 seconds, 50 ℃ 5 seconds, 60 ℃ 4 minutes, circulate and carries out PCR for 25 times and react.
3. add 18 μ l95% ethanol, ice bath is 10 minutes behind the mixing.
4.6000g centrifugal 30 minutes.
5. one deck snowflake paper in shop is inverted centrifugal 2 minutes of 700g.
6. add 2 μ l sample-loading buffers, go up sample behind the mixing, on PE company 377 sequenators, react,
Sequencing result is seen Fig. 1.Can accurately judge whether certified products.
Needing the present embodiment of explanation is a general rule, is not the fixed schedule of operation.In the actually operating, often change to some extent, otherwise can not reach expected results with different on dna profiling and the design of primers.The operational condition of this strictness, has no way of breaking a code because of the anti-person of emitting does not know reaction conditions for false proof very favourable again.
Claims (5)
1. one kind is utilized the nucleic acid sequencing analytical technology that nucleic acid is applied to false proof method, it is characterized in that
With nucleic acid or its segment, be fixed on and be used as anti-fake mark on the solid phase carrier, utilize the nucleic acid sequencing analytical technology check true and false.
2. anti-fake mark as claimed in claim 1 is characterized in that described anti-fake mark can directly be fixed on any solid-state object.
3. anti-fake mark as claimed in claim 1 is characterized in that described anti-fake mark can add protective layers such as covering plastics film or trehalose.
4. solid phase carrier as claimed in claim 1 is characterized in that described solid phase carrier can be natural or the solid-state material of organic or inorganic such as regenerated fiber, paper, film, leather, plastics, porous particle, powder, resin, Paraffin Wax Semi Refined, pottery, glass.
5. the method for the checking security mark true and false as claimed in claim 1 is characterized in that can judging the true and false of anti-fake mark according to the comparison of the sequencing result and the known nucleic acid sequence of nucleic acid on the anti-fake mark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00125492 CN1344807A (en) | 2000-09-29 | 2000-09-29 | Method of applying nucleic acid sequencing analysis technology in anti-fake |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00125492 CN1344807A (en) | 2000-09-29 | 2000-09-29 | Method of applying nucleic acid sequencing analysis technology in anti-fake |
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| Publication Number | Publication Date |
|---|---|
| CN1344807A true CN1344807A (en) | 2002-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00125492 Pending CN1344807A (en) | 2000-09-29 | 2000-09-29 | Method of applying nucleic acid sequencing analysis technology in anti-fake |
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|---|---|
| CN (1) | CN1344807A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604964A (en) * | 2015-02-09 | 2015-05-13 | 艾启华 | Biopesticide for grape plantation |
-
2000
- 2000-09-29 CN CN 00125492 patent/CN1344807A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604964A (en) * | 2015-02-09 | 2015-05-13 | 艾启华 | Biopesticide for grape plantation |
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