CN1343495A - Jadeite extracting agent and its prepn. method - Google Patents
Jadeite extracting agent and its prepn. method Download PDFInfo
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- CN1343495A CN1343495A CN00127073A CN00127073A CN1343495A CN 1343495 A CN1343495 A CN 1343495A CN 00127073 A CN00127073 A CN 00127073A CN 00127073 A CN00127073 A CN 00127073A CN 1343495 A CN1343495 A CN 1343495A
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- aeschna melanictera
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Abstract
The present invention provides a jadeite extracting agent for increasing metabolism of human body and curing disease. In the process of obtaining the product. The nephrite jadeite ore will be preprocessed with smashing selecting and washing then to heat and biol the mixer being mixed as the ratio of 1:4 with germ free distilled water in the evaporator at the temperature of 100 deg.c or above and the eluted compositions out from jadeite ore will be led into distilled water which is evaporated and converted to be a distilled liquid by the heat exchanger.
Description
The present invention relates to a kind of jadeite extracting agent and its a kind of preparation method, and more particularly, relate to nephrite Aeschna melanictera by the superfine crossed fiber microstructure that mixes pure distilled water and cryptocrystal tremolite type, be used for obtaining to be used to prepare a kind of a kind of jadeite extracting agent of nephrite Aeschna melanictera retort solution, this cryptocrystal tremolite is from a nephrite Aeschna melanictera ore deposit (Chunchon, Korea) exploitation, with its distillation and change the temperature of consequent nephrite Aeschna melanictera steam.
As everyone knows, Aeschna melanictera can be divided into jadeite and nephrite Aeschna melanictera substantially, and jadeite belongs to pyroxene series and is the monoclinic system that contains silicic acid, aluminium oxide and sodium carbonate.It is a kind of tightly packed material, and its hardness is equally matched with quartzy hardness.It is transparent or black, bluish-green or green translucent.People often are called jadeite " Aeschna melanictera ".The nephrite Aeschna melanictera is a kind of inorganic matter that has the monoclinic system chain silicate.The quality of nephrite Aeschna melanictera is determined that by fine structure fiber is thin more, quality good more (resource sales promotion 1993, Korea S resource company).
According to German document Mauda Palmer Die Verborgeng, " the technology outline of crystal and gem ", two kinds of different Ores, jadeite and nephrite Aeschna melanictera, as other gem of great majority, the two all contains silicon and oxygen, and they are made up of many crystal and the particle aggregate that contains calcium, ferrum and magnesium elements, it is located the human body beneficial, and jadeite then contains the composition of sodium and aluminum.Therefore, report recently, when the nephrite Aeschna melanictera contacts with human body, can provide very big curative effect the treatment of hypertension, diabetes, blood circulation disease, heart disease and renal dysfunction.
A kind of classical works of traditional oriental medicine, " oriental medicine's classical collection " have been if described Aeschna melanictera has been joined in the black rice wine, and the Aeschna melanictera powder that wine can be resolved into water and suck the sesame seed size is good for the drainage of refuse.It has also been described when with Aeschna melanictera powder (1 parts by volume), and rice (1 parts by volume) is when steaming and decocting becomes rice in the bronze, brass or copper ware ware with water in White Dew (1 parts by volume), and the Aeschna melanictera powder can become water (thereby being Aeschna melanictera liquid, " Aeschna melanictera god water ")." legendary god of farming plant ", " the Tang Dynasty plant " and " basic plant catalogue " have been described the Aeschna melanictera powder that sucks the sesame seed size and can moisten the vital organs of the human body and thoroughly get rid of refuse.In addition, it is by removing heat can exert an influence to digestive system from stomach, and for treatment bronchial asthma, body burning and pressure in chest and thirsty being good.When taking the Aeschna melanictera powder for a long time, it is comfortable and light that health can become, and pulmonary function is improved, and sounding by vocal cords can be easier.It also is good to throat, the function of nourishing hair, the vital organs of the human body and treatment neuropathy (as anxiety).
In addition, the composition of nephrite Aeschna melanictera goes out fabulous curative effect to physical manifestations and is free from side effects.For example, the inhalation (inhalatio) of white Aeschna melanictera powder is beneficial and rub on injured skin with the nephrite Aeschna melanictera and can remove scar in several days to muscular tone or angor.
Yet, because the nephrite Aeschna melanictera can not be present in occurring in nature with very big amount, although thereby its good medical curative effect well-known, its purposes is limited in individual jewelry accessories, as necklace, ring, bracelet or the like.In addition, the processing request of nephrite veteran expert work meticulously, and the price height of nephrite Aeschna melanictera, is disadvantageous economically, so use the nephrite Aeschna melanictera also to need in depth to develop as practical usually commodity.
Owing to notice the good medical curative effect of above-described nephrite Aeschna melanictera, applicant of the present invention has in depth studied many years and has obtained several patents in Korea S and other country, comprise Korean Patent NO.112812, its title is " containing the glass of nephrite Aeschna melanictera powder and its preparation method ".
So, the purpose of this invention is to provide and be used for obtaining to be used to prepare a kind of a kind of jadeite extracting agent of nephrite Aeschna melanictera retort solution and its preparation method, it is by mixing pure distilled water and jadeite ore (the cryptocrystal tremolite type of superfine crossed fiber microstructure) with about 1: 4 mixed proportion in vaporizer, heating blends at high temperature, make the steam that produces therefrom carry out heat exchange the mixture steam is transformed into nephrite Aeschna melanictera retort solution, it is a kind of jadeite extracting agent, thereby show pathological symptom (headache, feeling of numbness, dyspepsia, insomnia or the like) therapeutic effect, and can promote the growth of plant and animal.
In order to finish above-mentioned purpose of the present invention, a kind of jadeite extracting agent that is used for obtaining a kind of nephrite Aeschna melanictera retort solution is provided, and make the nephrite jadeite ore carry out pretreatment by pulverizing, selection and cleaning, and in vaporizer, mix with 1: 4 mixed proportion with sterile distilled water, this mixture is heated under 100 ℃ high temperature or higher temperature, the component of elution enters into just at ebullient distilled water from jadeite ore, with distilled water it is evaporated, steam is transformed into distillate by heat exchange.
On the other hand, the invention provides the method for a kind of jadeite extracting agent of preparation, its step comprises jadeite ore is ground into predetermined size, selection and cleaning Ore, to finish the preprocessing step, the preparation distilled water, mixed proportion with 1: 4 in a vaporizer is mixed good jadeite ore and the distilled water of preprocessing, and it is heated under 100 ℃ high temperature or higher temperature, so that the material that distilled water is advanced in contained elution in the jadeite ore is evaporated with distilled water, the steam of contained material is transformed into distillate in the jadeite ore to make containing of producing through heat exchange in evaporation step again.
Jadeite ore is σ here
18The cryptocrystal tremolite of O negative value, its contained component is as follows:
The semi-quantitative analysis (%) of the nephrite Aeschna melanictera powder that uses in＜the present embodiment 〉
Silicon 34 stannum 0.024
Calcium 4.9 silver medals 0.0013
Ferrum 0.23 titanium 0.0038
Aluminum 0.16 nickel 0.0028
Copper 0.17 chromium 0.0030
Cobalt 0.046 other element 0
Manganese 0.14
According to the mixed proportion of jadeite ore and distilled water, the jadeite ore that contains in the mixture is many more, and the effect of Aeschna melanictera improves greatly more.Yet, increase owing to costing an arm and a leg of Aeschna melanictera makes manufacturing cost.Therefore, jadeite ore and distilled water preferably mix with 1: 4 mixed proportion.
Distilled water among the present invention does not have particular restriction, just requires except not containing the impurity of the performance that may limit the jadeite ore that is fit to use in the distilled water.
By at a detailed description of the preferred embodiments and in conjunction with the accompanying drawings, it is more obvious that above-mentioned purpose of the present invention and advantage will become.
Fig. 1 is the device structure sketch map that is used for preparing a kind of jadeite extracting agent according to the present invention;
Fig. 2 represents the crystal structure of the Aeschna melanictera powder that uses in the present invention that records by scanning electron microscope (SEM);
The far-infrared radiation rate of the nephrite Aeschna melanictera that uses among Fig. 3 explanation the present invention by the Fourier transformation infrared spectrometer measurement;
Fig. 4 A and 4B are the standard solution (A) that uses among the present invention and the typical chromatograph of cryptocrystal tremolite microstructure sample (B);
The structure chart of the cryptocrystal tremolite microstructure that detects in the striatum that Fig. 5 represents in the present invention (last figure) and (figure below) uses in Fu Weihe.
Fig. 6 illustrates that the nephrite jadeite extracting agent discharges the dopamine role to the methamphetamine in the striatum that uses among response the present invention.
Fig. 7 illustrates the influence of the dopamine that the nephrite jadeite extracting agent discharges the nicotine in the striatum that uses among response the present invention.
After the Aeschna melanictera water that uses among the present invention is drunk in Fig. 8 explanation, the variation of control experiment group (rat) on activity;
Fig. 9 A and 9B are the SEM figure of the silk handled of the Aeschna melanictera water that uses among the present invention of no use:
Fig. 9 C and 9D are the SEM figure of the silk handled of the Aeschna melanictera water that uses among the present invention of no use;
Figure 10 represents the change in volume of whole culture medium of the Folium Digitalis Lanatae cultivated;
Figure 11 represents the variation of Folium Digitalis Lanatae cell volume;
Figure 12 represents the variation of the biologic artifact weight of cell;
Figure 13 represents the variation of dry cell weight amount;
Figure 14 represents the variation of the pH value in the Folium Digitalis Lanatae culture medium;
After Figure 15 represents to add nephrite Aeschna melanictera powder, the variation of the pH value in the culture medium;
Figure 16 represents the condition of analytical tool;
Figure 17 represents the analysis result exported;
Figure 18 is illustrated in the common pipe feedwater, the variation of the pH value of Aeschna melanictera necklace and jadeite ore;
Figure 19 represents the passing according to the time, the variation of the Aeschna melanictera necklace in the subsoil water and the pH value of jadeite ore;
Figure 20 represents the variation with the pH value of the strong acid solution of the Aeschna melanictera powder-processed among the present invention;
Figure 21 represents the passing according to the time, is stored in the milk bottle of the Aeschna melanictera powder that contains among the present invention and the variation of the pH value of the strong acid solution in the common milk bottle.
The description of preferred embodiment
The preferred embodiment and its preparation method of a kind of jadeite extracting agent of the present invention are described with reference to accompanying drawing.
Fig. 1 is the structural representation that is used for preparing an a kind of evaporation equipment 100 of jadeite extracting agent according to the present invention, in addition, and with the embodiment of a preparation jadeite extracting agent describing in view of the above.
At first, the nephrite jadeite ore that uses common pulverizer will be taken from the Chunchon, Korea (can be described as σ
18The cryptocrystal tremolite of O negative value) pulverizes, choose, clean then, to finish the preprocessing step by selection technology.
The pure distilled water 104 of jadeite ore after the preprocessing 102 and prepared beforehand is put into a vaporizer 106 with 1: 4 ratio.Then, start the flame-thrower nozzle 108 be placed on vaporizer 106 bottoms, under 100 ℃ high temperature or higher temperature, the heat that is produced with flame-thrower nozzle 108 is heating fumigators 106 constantly.So the thing of vaporizer 106 the insides seethes with excitement consumingly.During boiling, the component that goes out from jadeite ore 102 elutions enters the distilled water 104, as silicon, magnesium, calcium or the like, and along with distilled water 104 evaporates, flows out from discharging conduit 110 then.When by discharge conduit 110, owing to the temperature official post steam between in discharging conduit 110 and vaporizer liquefies, and flow out along passage, splash into the collection basin of collection usefulness then.From discharging steam flow that conduit 110 passes through when being installed near the heat exchanger 120 of discharging the conduit 110 emptying ends, carry out heat exchange, then liquefaction fully when directly herein.Herein, along with the growth of evaporation time, it is necessary constantly replenishing distilled water.Since heat exchanger 120 has an overall structure chart, so do not provide its detailed explanation.The jadeite extracting agent that use obtains according to top description can promote the metabolism of human body, and the treatment disease is made for example Ringer's mixture, as glucose.Also can be by minor amounts of additives and pigment or other coloring agent are joined in the jadeite extracting agent, with it (for example at health care (motion) ion beverage, Gator-Ade or Pacari-Sweat), the dietary fiber beverage (for example, Miero-Fiber), use in nutritional supplement (for example, Bacchus or Wonbi-D) and other the soda pop.Experimental result is as follows.
Embodiment in table 1～4 is used for studying the influence to life entity of the effect of the nephrite Aeschna melanictera that uses among the present invention and nephrite Aeschna melanictera below.
Table 1
＜lead content experiment 〉
| Sample | The Aeschna melanictera powder |
| Outward appearance | White powder |
| Job number | The academy IW 091394-1 that is attached to U.S. food Drug Administration |
| Experimental technique | Atomic absorption analysis |
| The result | Do not detect |
Table 2
The experiment of＜heavy metal (comprising lead) 〉
| Sample | The Aeschna melanictera powder |
| Outward appearance | White |
| Job number | ????IW?080894-4 |
| Experimental technique | ????USP?23 |
| The result | Do not detect |
Table 3
The experiment of＜dissolved inorganic matter 〉
In a reactor,, analyze extract with 1 liter water extraction sample (100g).
| Sample | The Aeschna melanictera powder |
| Outward appearance | White |
| Job number | ????IW?080894-4 |
| Experimental technique | List below |
| The result | List below |
Table 4
| Analyte | Result (ppm) | Detectable limit (ppm) |
| ????As | ????ND | ????0.05 |
| ????Ba | ????ND | ????0.20 |
| ????Cd | ????0.006 | ????0.005 |
| ????Cl | ????ND | ????1 |
| ????Cr | ????ND | ????0.01 |
| ????Cu | ????ND | ????0.05 |
| ????Fe | ????ND | ????0.10 |
| ????Pb | ????ND | ????0.05 |
| ????Mn | ????ND | ????0.02 |
| ????Hg | ????ND | ????0.0005 |
| ????NO 3 | ????ND | ????0.1 |
| ????Se | ????ND | ????0.05 |
| ????Ag | ????ND | ????0.01 |
| ????SO 4 | ????1.85 | ????1 |
| ????Zn | ????ND | ????0.01 |
(ND=does not detect, or concentration is lower than detectable limit)
Shown in result of upper experiment, since the nephrite Aeschna melanictera of Shi Yonging does not comprise the material of any murder by poisoning human body in the present invention, as lead, heavy metal or other material, provable is safe when it is used for plant or animal.
Carry out the research (Fig. 2) of the infrared spectrum of nephrite Aeschna melanictera powder in the present embodiment
Table 5
| Sample | Nephrite Aeschna melanictera powder |
| Outward appearance | White |
| Experimental technique | ????Perkin?Elmer?137 |
| The result | Describe below |
Infrared condition:
Phase: the oxolane thin film of liquefaction
The result: infrared spectrum is consistent with the polycarbonate resin pattern, and emits the electromagnetic wave of 6～52 μ wavelength.
Shown in following table 6 and 7, detect the COD (chemical oxygen demand) and the BOD (biochemistry oxygen demand) of the Aeschna melanictera powder that uses in the present embodiment.
Table 6
| Sample | The Aeschna melanictera powder |
| Outward appearance | White |
| Experimental technique | Standard method |
| The result | Describe below |
Table 7
| Water (control experiment) | Aeschna melanictera is handled | |
| 5 days BOD | ????224mg/l | ????233mg/l |
| ??COD | ????115mg/l | ????110mg/l |
Table 8
＜skin irritant research 〉
| Sample | The Aeschna melanictera powder |
| Outward appearance | White powder |
| Experimental technique | Describe below |
| The result | Describe below |
Method: federal registration, Vol.43, No.163
CTFA (cosmetics, washing product and fragrance association, Washington) technical manual step: this step utilization the albinism rabbit that be scratched with intact skin surface on utilize the patch test technology.Cut off the hair from back to the abdominal part.Two positions of being appointed as patch each other at a distance of the zone of about 10cm with back.In the patch zone, a zone scratch is formed four less epidermis cutting mouths (perpendicular with " tic-tac-toe " pattern in twos).Use thin rubber band and adhesive tape that 1 square inch surgical textiles is fixed on the described position as patch.Below every patch, introduce the sample of 0.5ml (g).Wind up with whole trunks of rubberized fabric animal, and exposure 24 hours.During exposure, animal is held onto.Remove after the patch reaction that each position of assessment produces on the basis of the weight record that is described below.
The assessment erythema of dermoreaction and the formation of eschar
No erythema-0
Very slight erythema (almost can't see)-1
The erythema-2 that profile is clear and definite
Medium serious erythema-3
Serious erythema (Radix Betae redness) is to the formation of formation (the infringement degree of depth)-4 edema of slight eschar
No edema-0
Very slight edema (almost can't see)-1
Slight edema (the marginal swell profile in zone is clear and definite)-2
Moderate edema (swell about 1mm)-3
Table 9
| Rabbit | 24 hours | 72 hours | ||||||
| Intact | Abrasive | Intact | Abrasive | |||||
| Erythema | Edema | Erythema | Edema | Erythema | Edema | | Edema | |
| # | ||||||||
| 1 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 |
| #2 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 |
| #3 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 |
| #4 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 |
| #5 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 |
| #6 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 | ?0 |
Conclusion: therefore this sample is suitable for using as cosmetics in the product of nursing skin through regulating the stimulus object that does not belong to skin.
Table 10
＜acute oral toxicity (0.5g/100g weight) experiment 〉
| Sample | The Aeschna melanictera powder |
| Outward appearance | White powder |
| Experimental technique | Describe below |
| The result | In table 11, describe below |
Table 11
| Rat | Sex | Initial weight (g) | Dosage (ml) | Weight afterwards (g) | Toxicity |
| ??1 | Female | ????207 | ????1.0 | ????219 | Do not have |
| ??2 | Female | ????211 | ????1.1 | ????226 | Do not have |
| ??3 | Female | ????215 | ????1.1 | ????225 | Do not have |
| ??4 | Female | ????208 | ????1.0 | ????217 | Do not have |
| ??5 | Female | ????200 | ????1.0 | ????213 | Do not have |
| ??6 | Male | ????227 | ????1.1 | ????235 | Do not have |
| ??7 | Male | ????216 | ????1.1 | ????224 | Do not have |
| ??8 | Male | ????205 | ????1.0 | ????218 | Do not have |
| ??9 | Male | ????210 | ????1.1 | ????224 | Do not have |
| ??10 | Male | ????212 | ????1.1 | ????221 | Do not have |
The preparation of sample: boil to boil with aseptic distilled water and extracted sample 10 minutes.By a sleeve pipe,, take extract to animal with the weight of 0.5g extract/100g animal.
Conclusion: this samples met standard-required, it should not have oral toxicity.
By being attached to nuclear medicine branch in the Samsung medical centre of the Sungkyunkwan of Seoul, South Korea university medical college, finish following experiment (embodiment 4), with the effect of research by the nephrite Aeschna melanictera of above-mentioned embodiment acquisition, promptly the result based on the foregoing description proves the influence of a kind of jadeite extracting agent to brain.Experimental technique and result are as follows.
Embodiment 4A. according to jadeite extracting agent of the present invention to the response methamphetamine and the influence of the dopamine that discharges
1) laboratory animal
Use one Lai Shi diabetic mice in male this pula (280～320g, Korea S's Experimental Animal Center) to finish interior micro-dialysis experiment.Animal is placed in 12: 12 the room of bright/dark condition, it is contacting foodstuff and water freely.
2) interior micro-dialysis experiment
A) Drug therapy
In order to investigate jadeite extracting agent to the response methamphetamine and the influence of the dopamine that discharges, according to the present invention, before at peritoneal injection methamphetamine (10mg/Kg, intraperitoneal), the jadeite extracting agent of peritoneal injection 0.5ml in 60 minutes is collected the dialysate sample from striatum then.
B) administration and micro-dialysis
With pentobarbital anaesthesia experiment animal (50mg/Kg, intraperitoneal), then in non-invasive mode, according to Paxinos﹠amp; Watson collection of illustrative plates (1986) inserts a conduit in the striatum (real taxis is coordinated: AP, 1.0 and L, 3.2 with respect to anterior fontanelle, H, 3.0 with respect to pachymeninx), and is fixed with a filament and tooth bonding agent.After 24 hours convalescent period, from conduit insert a 4mm vertical micro-dialysis probe (CMA-12, Carnegie-Medicine, Stockholm, Sweden).The inlet of micro-dialysis probe links to each other with syringe on being installed in flow pump by the liquid in rotation ring of a bilayer.When with 1.5 μ l/ minutes flow velocity, carry from the micro-dialysis probe and to contain 145mM NaCl, 2.7mM KCl, 1.2mMCaCl
2, 10mM MgCl
2, 2.0mM Na
2HPO
4, during the artificial cerebrospinal fluid of PH7.4, every 20 minutes, the trace distillate collector that uses an outlet that provided by Camegie-Medicine and the micro-dialysis probe to link to each other collected dialysate.Carry out the micro-dialysis experiment on one's body in the laboratory animal that is allowed to move freely,
C) analysis of micro-dialysis sample
Survey the metabolism of monoamine and dialysate sample by HLPC electrochemical detection (ECD) system.(4 μ m, 150 * 3.9mm) isolate dialysate to use an anti-phase Waters Nova-Pak C-18 pillar.Mobile phase is by the sodium phosphate of 75mM, the EDTA of 0.12mM, and the octyl sulfate of 1.4mM and 10% acetonitrile are formed, and it is adjusted to PH3.2 with phosphoric acid.The Electriochemical measuring sensor that use has an ESA coulochemII 5200A of high-performance analytical tool (ESA type 5014) is analyzed the peak value of HPCL.The electromotive force of working electrode reaches+320mV.The flow velocity of system is 1.0ml/ minute.
Fig. 4 represents from the typical chromatograph of standard solution and the acquisition of micro-dialysis sample.Can isolate dopamine significantly, dihydroxyphenyl acetic acid (DOPAC), 4-hydroxy-3-methoxy-.alpha.-toluic acid. (HVA), 5-hydroxy tryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA).The dopamine concentration of dialysate exceeds 3 times than minimum measuring limit (as 116pM), and the basic concentration of dopamine, DOPAC, HVA, 5-HT and 5-HIAA is respectively 18.5nM, 90.5nM, 74.7nM, 6.2nM and 45.6nM.Because the dopamine resume speed of micro-dialysis probe is 20% (being obtained by other experiment), so estimate that the levels of dopamine of micro-dialysis probe is 92.5nM.The percentile meansigma methods of benchmark of the dopamine concentration of three samples just having collected before the experimental therapy is expressed as the levels of dopamine of micro-dialysis probe.
D) histology identifies
When experiment finishes, with laboratory animal anesthesia, pass through the formalin solution of infusion normal saline and 10% then with decerebration with excessive pentobarbital.The brain that is excised was preserved for 2 weeks in 10% formalin solution at least.Then, the residing cell tissue of micro-dialysis probe is partly cut to the thickness of 50 μ m, and (see figure 5) can be discerned by brazilwood extract dyeing in their tram.
E) statistical analysis
All measured values all are expressed as mean square deviation.Use the multipole analysis of variable (medicine * time) and the statistical significance that the Bonferroni method is come analytical data.Use as the software StarView 4.02 of statistical analysis (Abacus Concepts, Inc., Berkely, CA, U.S.A.) and macintosh computer.Mensuration is based on the statistical significance of 0.005 P level.
3) result of study
Fig. 6 represents that the nephrite jadeite extracting agent discharges the influence of dopamine to the methamphetamine in the striatum that uses among response the present invention.After injection methamphetamine (10mg/Kg is on the peritoneum), the extracellular dopamine concentration increases gradually, arrive after 60 minutes peak (for benchmark 433.6 ± 14.3%).Before the infusion methamphetamine 60 minutes, when the jadeite extracting agent of peritoneal injection 0.5ml, the increase of the extracellular dopamine concentration in the striatum tapers to 32.8% with respect to methamphetamine (benchmark 29.15 ± 11.4%).The inject delay of jadeite extracting agent require to reach time of the maximum horizontal of the dopamine after the infusion methamphetamine.Can not influence extracellular dopamine concentration in the stable striatum according to jadeite extracting agent of the present invention.B jadeite extracting agent according to the present invention is to the influence of the dopamine that discharges of response nicotine
1) laboratory animal
Use one Lai Shi diabetic mice in male this pula (280～320g, Korea S's Experimental Animal Center) to finish interior micro-dialysis experiment.Animal is placed in 12: 12 the room of bright/dark condition, it is contacting foodstuff and water freely.
2) interior micro-dialysis experiment
A) Drug therapy
In order to investigate jadeite extracting agent to the response nicotine and the influence of the dopamine that discharges, according to the present invention, before at peritoneal injection nicotine (0.4mg/Kg, subcutaneous injection), the jadeite extracting agent of peritoneal injection 0.5ml in 60 minutes is collected the dialysate sample from striatum then.
B) administration and micro-dialysis
With pentobarbital anaesthesia experiment animal (50mg/Kg, intraperitoneal), then in non-invasive mode, according to Paxinos﹠amp; Watson collection of illustrative plates (1986) inserts a conduit in the striatum (real taxis is coordinated: AP, 1.0 and L, 3.2 with respect to anterior fontanelle, H, 3.0 with respect to pachymeninx), and is fixed with a filament and tooth bonding agent.After 24 hours convalescent period, from conduit insert a 4mm vertical micro-dialysis probe (CMA-12, Carnegie-Medicine, Stockholm, Sweden).The inlet of micro-dialysis probe links to each other with syringe on being installed in flow pump by the liquid in rotation ring of a bilayer.When with 1.5 μ l/ minutes flow velocity, carry from the micro-dialysis probe and to contain 145mM NaCl, 2.7mM KCl, 1.2mMCaCl
2, 10mM MgCl
2, 2.0mM Na
2HPO
4, during the artificial cerebrospinal fluid of PH7.4, every 20 minutes, the trace distillate collector that uses an outlet that provided by Carnegie-Medicine and the micro-dialysis probe to link to each other collected dialysate.Carry out the micro-dialysis experiment on one's body in the laboratory animal that is allowed to move freely,
C) analysis of micro-dialysis sample
Survey the concentration of micro-dialysis liquid sample by HLPC electrochemical detection (ECD) system.(4 μ m, 150 * 3.9mm) isolate dialysate (30 μ l dosage) to use an anti-phase Waters Nova-Pak C-18 pillar.Mobile phase is by the sodium phosphate of 75mMV, the EDTA of 0.1mM, and the octyl sulfate of 1.4mM and 10% acetonitrile are adjusted to PH3.2 with phosphoric acid.The Electriochemical measuring sensor that use has an ESA coulochem II 5200A of high-performance analytical tool (ESA type 5014) is analyzed the peak of HPCL.The electromotive force of working electrode reaches+320mV.The flow velocity of system is 1.0ml/ minute.The benchmark common size statement of the average dopamine concentration of three samples collecting fast immediately before the experimental therapy is shown as the levels of dopamine of micro-dialysis probe.
D) histology identifies
When experiment finishes, with laboratory animal anesthesia, pass through the formalin solution of infusion normal saline and 10% then with decerebration with excessive pentobarbital.The brain that is excised was preserved for 2 weeks in 10% formalin solution at least.Then, the residing cell tissue of micro-dialysis probe is partly cut to the thickness of 50 μ m, and their tram can be discerned by brazilwood extract dyeing.
E) statistical analysis
All measured values all are expressed as mean square deviation.Use the multipole analysis of variable (medicine * time) and the statistical significance that the Bonferroni method is come analytical data.Mensuration is based on the statistical significance of 0.005 P level.
3) result of study
Fig. 7 represents that nephrite jadeite extracting agent (distillate) discharges the dopamine role to the methamphetamine in the striatum that uses among response the present invention.After injection nicotine (0.4mg/Kg, subcutaneous injection), the extracellular dopamine concentration increases gradually, arrive after 40 minutes peak (for benchmark 403.9 ± 33.2%), after 120 minutes, reduce then and return to datum-plane.Before the infusion nicotine 60 minutes, when the jadeite extracting agent of peritoneal injection 0.5ml, the increase of the extracellular dopamine concentration in the striatum with respect to nicotine (for benchmark 193.9 ± 6.1%) taper to 52.0%.The inject delay of jadeite extracting agent need to arrive time (behind the injection nicotine 80 minutes) of the maximum horizontal of the dopamine after the infusion nicotine.Jadeite extracting agent of the present invention can not influence the extracellular dopamine concentration in the stable striatum.
Aforesaid experimental result shows that because methamphetamine and nicotine in the striatum of diabetic mice, jadeite extracting agent of the present invention has suppressed the release of dopamine.
Experiment (embodiment 5 and 6) is in order to study jadeite extracting agent among the present invention to the breeding of diabetic mice and the influence of growth, and its Aeschna melanictera comprises the supernatant Aeschna melanictera water that obtains after blank solution (Space) and the Aeschna melanictera powder precipitation.Experimentize by Korea Food Res Inst.Below details are described in.
In order to study of the influence of nephrite Aeschna melanictera, experimentize by Korea Food Res Inst to the streptozotocin induced symptom that alleviates the diabetic mice diabetes.Below details are described in.
(body weight 252～294g) is supplied with in intraperitoneal can bring out the streptozotocin (35～40mg is dissolved in the citrate buffer solution of the PH4.0 of 0.1M) of diabetes to one Lai Shi diabetic mice of 32 Si Pula.The dispensing streptozotocin is after seven days, and the blood sugar level of rat is higher than 250mg/dl based on urine paper (Eiken Chemical Co.Ltd., Japan), and it is selected as benchmark.Finish four groups of experiments at 50 days experimental session, every group of 5 rats.Every day is to the injection distillatory Aeschna melanictera water of 1.0ml (pH6.4) in the A group rat peritoneum.In whole experiment, B is organized the rat nursing be placed with in the cage of Aeschna melanictera product in the bottom.Every day, the supernatant Aeschna melanictera water after with the Aeschna melanictera powder precipitation was organized rat as the drinking water feeding to C.D group rat is in contrast only to its injection streptozotocin.The diet of feeding is that commercial rat is omnivorous, and looks after rat according to common mode.At the 50th day rat is slaughtered, and collected blood from abdominal aortic.At room temperature place blood after 30 minutes, the rotating speed with 5000rpm in 15 minutes prepares blood plasma from the blood centrifugal machine that contains glycogen.The organ (liver, kidney) that takes out is weighed and record.Detected detection thing is HBA (a beta-hydroxy-butanoic acid ester blood plasma) in blood plasma, FFA (free fatty acid), cholesterol, HDLH-cholesterol and triglyceride.Come the content of quantitative analysis HBA by the absorption (OD) of measuring N ADH, by producing NADH at 340nm oxidation beta-hydroxy-butanoic acid ester dehydrogenase.Use the lipids in clinical utensil (Eiken ChemicalCo., Ltd, Japan) the measurement blood.
Table 12
The influence that the body weight of the diabetes rat that＜Aeschna melanictera brings out streptozotocin and organ weight change 〉
| Group number | Body weight (g) | Organ weight's (g/100g body weight) | ||
| Beginning | Finish | Liver | Kidney | |
| ??A | ?270.4±11.3 | ?271.7±49.4 | ?4.31±0.33 | ?1.02±0.12 |
| ??B | ?284.8±6.1 | ?279.6±20.4 | ?4.30±0.64 | ?1.01±0.18 |
| ??C | ?280.4±13.2 | ?315.7±41.1 | ?3.96±0.75 | ?0.874±0.14 |
| ??D | ?257.2±4.2 | ?194.8±26.3 | ?4.37±0.19 | ?0.78±0.04 |
Table 13
The influence that the index biochemical of the rat diabetes that＜Aeschna melanictera brings out streptozotocin changes 〉
P＜0.05
aBeta-hydroxy-butanoic acid ester (ketoboidies)
bFree fatty acid
cWhole cholesterol
dTriglyceride (neutral lipids)
eHDL-C
| Group number | Glucose (mg/100ml) | ????????HBA ?????(μmol/ml) | ????????FFA b??????(μeq/ml) | ??????TC c???(μmol/ml) | ??????TG d???(mg/100ml) | ??????HDL e???(mg/100ml) |
| ??A | ??639.2±99.0 a | ??0.782±0.481 a | ??507.6±226.6 a | ??166.2±69.4 | ??410.7±50.1 | ??32.35±5.94 |
| ??B | ??495.1±228.1 ab | ??0.527±0.296 ab | ??349.5±79.2 ab | ??161.3±45.3 | ??122.5±43.5 | ??29.49±1.7l |
| ??C | ??354.1±154.2 ab | ??0.425±0.172 ab | ??287.3±71.7 b | ??145.4±29.1 | ??87.9±29.2 | ??30.55±8.28 |
| ??D | ??196.8±16.3 a | ??0.276±0.036 b | ??291.0±131.2 b | ??158.2±12.2 | ??86.8±3.4 | ??31.37±3.84 |
With regard to body weight, the C of feeding Aeschna melanictera water group rat has shown the body weight higher than other group, in other group, when experiment finishes, lose weight (B, D) or remain unchanged (A).In the sick Mus of DM, the weight of kidney increases basically, and the trend of this increase also is present in the weight of liver, but the weight of the liver of the rat of feeding Aeschna melanictera water is minimum.The concentration of blood glucose, ketoboidies and FFA increase progressively according to the order of A group, B group, C group, D group.The concentration of plasma cholesterol and triglyceride has the trend identical with above-mentioned parameter (A＞B＞C＞D).From the result, different with other two experimental grouies, the concentration ratio that all parameter lists that record of the rat of feeding Aeschna melanictera water (C group) reveal blood cholesterol levels in relatively low value and the C group rat and FFA is lower without any the rat in the matched group of Aeschna melanictera inorganic agent.The concentration of TC and TG is according to order A＞B＞C＞D.The exponential result of glucemia changes according to researcher by blood glucose response after meal.Yet, the commercial mixed food of feeding (TMR) although in experiment, do not consider the glucemia index of corn, the C group rat of feeding Aeschna melanictera water demonstrates the effect that the hyperlipemia of one of diabetic symptom alleviates.Between experimental group, do not produce the difference of HDL-cholesterol concentration.Aeschna melanictera water is supplied with the diabetes rat that streptozotocin brings out, and it shows to stop and loses weight, the effect that the hyperlipemia of prevention kidney and one of liver increase and diabetic symptom alleviates.
In each the processing, select one Lai Shi rat of 30 Si Pula (ages in 9 weeks) and A is organized rat feed in the cage of the nephrite Aeschna melanictera that contains gas phase and supply with tap water.To the supernatant Aeschna melanictera water (2mg/ml tap water) after the B group rat feeding Aeschna melanictera powder precipitation, but the nephrite Aeschna melanictera of gas phase is not injected in the cage.C group rat is organized (no gas phase nephrite Aeschna melanictera and no Aeschna melanictera water) in contrast.In 9 days, make the rat copulation, male rat is isolated and slaughtered to carry out the test of sperm concentration and vigor then.The results are shown in the following table 14.
Table 14 (the Aeschna melanictera product is to the breeding of rat and the influence of growth 〉
| Group number | ???????A | ?????????B | ????????C |
| Childbirth (number of rat) | ???9 | ??9 | ??9 |
| Young how many (♂/♀) of Mus | ???96(57/39) | ??94(57/37) | ??102(54/48) |
| Young what (heads/♀ rat) of Mus | ???11.8±1.9 | ??10.9±1.4 | ??12.8±2.1 |
| Birth weight (g) | ???4.73±1.10 | ??5.79±0.95 | ??5.35±0.35 |
| Mortality rate (number) | ???4 | ??4 | ??3 |
| Natural law from copulation to childbirth | ???23.8±1.4 | ??25.1±1.7 | ??25.5±3.7 |
| Body weight during wean (g) | ???43.45±8.04 | ??42.73±8.76 | ??42.07±10.71 |
| Body weight when slaughtering (g) | ???294.0±l0.9 | ??274.4±8.6 | ??288.44±26.7 |
| Testicular weight (g) | ???3.904±0.21 | ??3.574±0.30 | ??3.99±0.15 |
| Sperm concentration (108/ml) | ???6.32±2.4 | ??4.80±1.3 | ??4.60±1.9 |
| Motility of sperm (%) | ???91.0±4.2 | ??92.0±2.7 | ??88.0±7.6 |
From find, Aeschna melanictera product and Aeschna melanictera water treatment agent have shown better childbirth record.Although C group rat has born more several mices, the parturition rate (90%) of A group and B group is than parturition rate (80%) height of C group.Yet, in this research, do not study the influence simultaneously of Aeschna melanictera product and Aeschna melanictera water treatment agent.In a word, as if the result hints out that Aeschna melanictera product and Aeschna melanictera water treatment agent can influence breeding and growth.Injected Aeschna melanictera product or more superior in breeding and growth with the group of Aeschna melanictera water treatment.
This experiment is to supply with the variation of rat on activity that is added with the nephrite Aeschna melanictera powder Aeschna melanictera water that uses among the present invention in order to study.
As shown in Figure 8, in the stable testing feature, the rat of the matched group of medication treatment begins to show the stability of its nervus centralis taking Chinese medicine after 5 minutes, return to normal state fully after 30 minutes then.Rat in common matched group shows the trend that weakens on the whole on its activity.The activity of rat in the group of drinking Aeschna melanictera water is similar to common matched group, and its average activity that shows is higher a little than common matched group, and apparently higher than the activity of medication treatment group.
This experiment is in order to study when supplying water in the formed depurator of nephrite Aeschna melanictera that uses from the present invention, Aeschna melanictera is for growth rate, the influence of the vigor of sperm and the amount of movement of muscle, this depurator obtains from Korea S spring river, with with AIN (AAN)-food storage in common refrigerator, feeding is to test group (white mouse).
With experimental applications in the common water purifier of feeding water and preserve the matched group (A) of the AIN-food in common refrigerator, the group B that handles with Aeschna melanictera then water and the AIN diet supplied from the Aeschna melanictera water purifier of feeding then is housed in the refrigerator that is formed by the Aeschna melanictera material.9 white mouse (ages in 10 weeks) are arranged in each test group, and (3 * 3 repeat) feeds 18 whole rats, growth rate, motility of sperm, the concentration and the quantity of motion of sperm concentration blood lipids.
Table 15
| The measurement result of characteristic | Processed group | |
| Matched group (A) | Processed group (B) | |
| The persistence blood PH TG TC HDL that food (g/ days) amount of drinking water (ml/ days) the sperm motility sperm life sperm concentration amount of movement swingle rotation basket of growth rate (g/ days) picked-up moves about | ????2.33±0.37 ????15.52±1.30 ????35.8±1.1?? ?? ????90.0±0.00 ????5.7±1.37?? ?? ????41.0±1.6 ????18 ????10,481±5,315?? ?? ????7.66±0.10 ????171.5±39.2 a????171.1±62.3 ????82.8±10.7 | ????2.64±0.41 ????14.97±1.07 ????27.8±0.9?? ?? ????88.3±2.9 ????6.9±1.08?? ?? ????41.5±9.1 ????17 ????11,713±3,519?? ?? ????7.84±0.09 ????133.1±33.3 b????146.6±62.1 ????69.3±7.4 |
A: common cage, water come from common water purifier and AIN-food storage in common refrigerator
B: have the cage of the tiling of Aeschna melanictera processing, water comes from the Aeschna melanictera water purifier and the AIT-diet is housed in the Aeschna melanictera refrigerator
P＜0.05: the statistical significance on 95% level
＜result 〉
1. two groups of handling are in parturition rate and blood lipids concentration and life
Do not show difference on the long rate.
2. do not have very big difference between two groups in the life-span of sperm, Aeschna melanictera was handled
Group in sperm concentration approximately high by 17% than matched group.
3. in measuring sperm life, the persistency of moving about in the group that Aeschna melanictera was handled is big
Be 1200 seconds approximately, average upward longer than matched group.
4. the pH of the blood in the group handled of Aeschna melanictera is more much higher than matched group.
5. the group handled than Aeschna melanictera of matched group shows in the higher blood lipids
The HDL level.
Shown in above-mentioned result, the group that Aeschna melanictera was handled is better than matched group on persistency of moving about and sperm concentration.Especially, the group handled of Aeschna melanictera shows the level of significantly lower TC and TG than matched group.
Confirm as table 16 and 17,, specify the white mouse at 5 ages in week to finish experiment in order to study different types of water to the childbirth of test group (white mouse) and the influence of wean.Drinking water is divided into three groups, that is: subsoil water (A) comes from the water (B) and the Aeschna melanictera distilled water of the present invention (C) of common water purifier.As the AIN-food of experimental foods equably feeding give experimental group.Each experimental group is specified 9 female rats (♀), and (3 * 3 repeat) amounts to 27 rats, and each experimental group is specified 8 male rats (♂), amounts to 24 rats.After around feeding, in 7 days, allow the rat copulation of experimental group, copulation ratio be ♂/♀=1: 3.Through the pregnancy in 3 weeks, in the time of weaning, feed the rat of experimental group from giving a birth to.
Table 16
＜different types of water is to the influence of white mouse 〉
1) measures A from male rat: subsoil water, B: come from the water of common water purifier, C: Aeschna melanictera distilled water
| The | Processed group | ||
| ???????A | ????????B | ????????C | |
| Food (g/ days) amount of drinking water (ml/ days) the motility of sperm sperm life sperm concentration (10 of weight (g/ days) picked-up that increases 8/ ml) quantity of motion is rotated persistency (second) swingle (second) the blood pH that basket (time, 10 minutes) moves about | ??4.32±0.26 ??16.25±0.32 ??20.3±1.2 ??91.1±2.3 ??4.01±1.09?? ?? ?? ??57.8±3.16 ??4,082±813 ??39.6±18.9 ??7.64±0.09 | ??4.20±0.26 ??16.51±0.45 ??22.00±1.08 ??90.5±2.8 ??4.26±0.71?? ?? ?? ??35.2±19.0 ??5,087±1,471 ??32.9±9.9 ??7.69±0.12 | ???4.33±0.37 ???17.01±1.06 ???19.8±1.1 ???91.1±2.8 ???4.87±1.41 ???±?? ?? ???33.3±14.7 ???5,332±445 ???31.5±11.0 ???7.78±0.24 |
Table 17
＜different types of water is to the influence of the childbirth of white mouse)
| Processed group 1) | Natural law from copulation to childbirth (my god) | Childbirth (rat number) (parturition rate, %) | Whole young Mus numbers | Young Mus number in the brood | The number of the young Mus of wean (♂/♀) |
| ????A | ??24.2±1.5 | ??5(55，6) | ????56 | ??11.2±1.9 | ??53(26/27) |
| ????B | ??25.5±2.3 | ??6(66，7) | ????46 | ??7.7±2.9 | ??39(17/22) |
| ????C | ??24.7±2.3 | ??7(77，8) | ????81 | ??11.6±0.5 | ??69(41/28) |
＜result＞
1. treated A group, the B group, in the C group, at rate of growth, amount of movement and
There is not tangible difference on the motility of sperm.
The sperm concentration of 2. treated C group is promptly with nephrite Aeschna melanictera distillation of the present invention
The group of solution-treated is 4.87 * 10
8/ ml is than other treated A group and B
Organize high 21.4% and 14.3%, A group and B group are respectively 4.01 * 10
8/ ml and 4.26
×10
8/ml。
3. the branch in subsoil water, the water that comes from common water purifier and Aeschna melanictera distilled water
The childbirth rate is respectively 55.6%, 66.7% and 77.8%, the parturition rate of the C group that Aeschna melanictera is handled
Higher significantly than other group.
4. the children in subsoil water, the water that comes from common water purifier and Aeschna melanictera distilled water
The quantity of Mus is respectively 11.2 ± 1.9,7.7 ± 2.9 and 11.6 ± 0.5.
5. in treated A, B and C group, divide young Mus that the puerperium weaned for 3 weeks
Quantity is respectively 53,39 and 69, and sex (♂/♀) ratio is respectively 26/27,
17/22 and 41/28, show that male juvenile mouse is than female children in the C group of handling with Aeschna melanictera
Mus is many.
This shows that the sperm concentration of Aeschna melanictera processed group is than other group difference high 21.4% and 14.3%.The parturition rate of the female white mouse of Aeschna melanictera processed group is 78%, and is higher than other group that is 56% and 67% respectively.And on the sex ratio of the young Mus of weaning, the male juvenile mouse of Aeschna melanictera processed group is more than other group.
Carry out this and test and study the filter that nephrite Aeschna melanictera used in the application of the invention makes and come the feedwater of purification pipe, the Aeschna melanictera treating water that obtains is to the (see figure 9) that influences of the growth of silkworm and the silk quality produced.
1. material and method
1. the kind of silkworm: white jade silkworm
2. trophophase: November is to December
3. growing method:, feed to temperature constant and the constant Mulberry of humidity in all silkworm age phases
Leaf
4. the number of silkworm: matched group 150 (H2 repetition)
Processed group 130 (H2 repetition)
5. handle:
Matched group: the Folium Mori that feeding sprays with distilled water.
Processed group: the Folium Mori that feeding sprays with the Aeschna melanictera treating water.
Handle the time of silkworm: since second length of time
Table 18
＜nursing the result that finished with Aeschna melanictera powder water treatment agent 〉
| Repeated experiments | The number of the larva that is studied | The larval phase in the 5th age | Whole larval phases | The percentage rate of nymphosis | ||||
| My god | Hour | My god | Hour | ??％ | Index | |||
| Matched group | ??1 | ????150 | ????7 | ????7 | ????23 | ????7 | ??91.3 | ??100 |
| ??2 | ????150 | ????7 | ????7 | ????23 | ????7 | ??76.6 | ||
| On average | ????150 | ????7 | ????7 | ????23 | ????7 | ??84.0 | ||
| Processed group | ??1 | ????130 | ????7 | ????7 | ????23 | ????15 | ??74.1 | ??85 |
| ??2 | ????130 | ????7 | ????7 | ????23 | ????15 | ??68.2 | ||
| On average | ????130 | ????7 | ????7 | ????23 | ????15 | ??71.3 | ||
Table 19
The wrapping wire performance of the Bombyx bombycis of＜usefulness Aeschna melanictera powder-processed agent 〉
| Silkworm silk length (m) | Silkworm silk weight (cg) | The size (d) of silk | No fracture of wire length (m) | No fracture of wire weight (cg) | Wrapping wire ability (%) | |
| Matched group | ??1,222 | ??33.7 | ??2.48 | ???3.47 | ??23.4 | ??69 |
| Processed group | ??1,283 | ??36.3 | ??2.55 | ???1,005 | ??28.5 | ??78 |
Table 20
The variation of＜larva the weight handled with Aeschna melanictera 〉
| Weight from the new larva of peeling that casts off a skin for the third time | Weight from the 4th the new larva of peeling that casts off a skin | The 3rd day the weight in the 5th age | Sophisticated larva | |
| Matched group | ????0.46 | ????1.85 | ????20.85 | ????47.4 |
| Processed group | ????0.47 | ????1.94 | ????23.03 | ????52.2 |
Table 21
The influence that＜Aeschna melanictera solution reduces blood sugar content 〉
| The silk extract | Blood sugar content | Influence to the blood glucose reduction | ||
| The N maltose (A) of the injection of injection silkworm extract (mg/100ml) | The silkworm extract (B) of injection (mg/l00ml) | Quantity (C=A! B) (mg/100ml) | Efficient | |
| Use water treatment | ??64.0∨1.87 | ??27.7∨1.70 | ????36.3 | ??56.7 |
| Use the Aeschna melanictera solution-treated | ??69.6∨1.62 | ??20.7∨1.62 | ????48.9 | ??70.3 |
＜result 〉
1. fail to come out on time the weight ratio matched group height of Aeschna melanictera processed group larva each silkworm.Especially, under the sophisticated situation of larva, the high 0.48g of weight ratio matched group of each larva of Aeschna melanictera processed group.
2. the percentage rate of Aeschna melanictera processed group nymphosis is 71.2%, and it is lower by about 15% than matched group.The productive rate of Aeschna melanictera processed group cocoon is that each thousand larva produces 15g, and it is lower by about 7% than matched group.
3. the weight of the single silkworm of Aeschna melanictera processed group is 2.14g, and it is higher by about 6% than matched group.The weight of Aeschna melanictera processed group Periostachum bombycis is 44.5cg.The percentage matched group of Aeschna melanictera processed group Periostachum bombycis is high by 2%.
4. the wrapping wire performance of Aeschna melanictera processed group is better than matched group generally.Especially, in the length of silkworm silk, the weight of silkworm silk and wrapping wire ability aspect, Aeschna melanictera processed group are excellent (than matched groups high about 9%).
5. aspect the quality of silkworm silk, the cleaning point of Aeschna melanictera processed group is higher a little than matched group.The toughness of Aeschna melanictera processed group, stretch and the percentage rate of raw silk all than other matched group height.Yet the productivity ratio matched group of cocoon is low in per 10000 larvas of Aeschna melanictera processed group.
6. between Aeschna melanictera processed group and matched group, on surface texture, do not observe substantial difference.
7. the crystal structure of Aeschna melanictera processed group is have sharp-pointed front end bar-shaped.
Below experiment (embodiment 11 to 15) be in order to study in the present invention the nephrite Aeschna melanictera that uses (obtain, and the also preparation thus of Aeschna melanictera water) to the germination of seed and the influence of growth from Korea S spring river.
A. the component analysis of nephrite Aeschna melanictera and Aeschna melanictera water
The component of nephrite Aeschna melanictera is taken from the Chunchon, Korea, uses PW 1,480 X RIW-Fuorescence Sequenflal spectrometer to analyze.Analysis result shows that the nephrite Aeschna melanictera contains the SiO as main component
2With several trace element to plant growing necessity.
B. supernatant Aeschna melanictera water and groundwater quality quantitative analysis
Analyze supernatant Aeschna melanictera water (subsoil water in an Aeschna melanictera ore deposit) and groundwater quality.Analysis result shows in the subsoil water in Aeschna melanictera ore deposit (for example supernatant Aeschna melanictera water) and does not find special trace element.
＜supernatant Aeschna melanictera water is to the comparison of the influence of germination 〉
1. select the seed of 20 full impatiens, Seem Lablab Album, rice, Radix Raphani and Caulis et Folium Chrysanthemi segetis to finish the experiment of germinateing, 8 toilet papers are placed on the Schale (experiment disc), put seed thereon, supply with the supernatant Aeschna melanictera water, subsoil water and tap water then.
2. supply with the seed of supernatant Aeschna melanictera water and supply with the seed of tap water that 50 meters of mutual edge distances are far away mutually, and placed 5 days separately at the dark place under the room temperature, to observe 5 times germination percentage.
3. in order to study the supply scope of supernatant Aeschna melanictera water, per 20 radish seeds are supplied with Aeschna melanictera water and subsoil water, and the Schale that is placed with seed coils respectively apart from 10m 20m, 30m, 40m, 50m.
Experimental result is as shown in table 22 and 23.
Table 22
＜usefulness supernatant Aeschna melanictera water, the comparison of the germination percentage of subsoil water and tap water 〉
| Supernatant Aeschna melanictera water | Subsoil water | Tap water | ||||||||||
| ??2 | ??3 | ??4 | ??5 | ??2 | ??3 | ??4 | ??5 | ??2 | ??3 | ??4 | ??5 | |
| Rice | ??10 | ??45 | ??85 | ??96 | ??6 | ??43 | ??76 | ??91 | ??4 | ??42 | ??74 | ??90 |
| Radix Raphani | ??27 | ??78 | ??90 | ??96 | ??26 | ??73 | ??87 | ??94 | ??24 | ??72 | ??86 | ??94 |
| Caulis et Folium Chrysanthemi segeti | ??20 | ??46 | ??64 | ??64 | ??18 | ??45 | ??60 | ??61 | ??18 | ??41 | ??61 | ??62 |
| Semen Pisi sativi | ??9 | ??23 | ??48 | ??51 | ??9 | ??21 | ??47 | ??51 | ??8 | ??20 | ??43 | ??52 |
| Caulis et Folium Brassicae capitatae | ??28 | ??79 | ??91 | ??91 | ??24 | ??73 | ??90 | ??90 | ??23 | ??71 | ??90 | ??90 |
The result hints out the germination cycle of supernatant Aeschna melanictera water five kinds of listed seeds above having accelerated.
Table 23
＜the comparison of Radix Raphani percentage of seedgermination when supplying with supernatant Aeschna melanictera water and subsoil water 〉
| Aeschna melanictera water | ???10m | ???20m | ???30m | ???40m | ??? | |
| 1 day | ????8 | ????7 | ????8 | ????5 | ????6 | ????6 |
| 2 days | ????27 | ????28 | ????29 | ????22 | ????21 | ????22 |
| 3 days | ????78 | ????79 | ????77 | ????75 | ????74 | ????73 |
| 4 days | ????90 | ????89 | ????89 | ????90 | ????89 | ????89 |
| 5 days | ????96 | ????95 | ????94 | ????93 | ????94 | ????93 |
The result hints out the germination cycle that the abnormal smells from the patient of Aeschna melanictera water is exerted one's influence and accelerated radish seed in about 20m.
The abnormal smells from the patient of Aeschna melanictera porcelain is to the comparison of the influence of germination
Select the seed of 20 full impatiens, Seem Lablab Album, rice and Caulis et Folium Chrysanthemi segetis to finish the experiment of germinateing, 8 toilet papers are placed on five Aeschna melanictera porcelain and 25 Schales, put seed thereon, respectively apart from 10m, 20m, 30m, 40m, 50m.The result who at room temperature observes germination is to measure the scope (seeing Table 24) of abnormal smells from the patient.
1. at the dark place seed was placed 5 days separately under the room temperature, to observe 5 times germination percentage.
2. statistical value is based on 5 times whole (100%).
Table 24
The comparison of＜usefulness Aeschna melanictera porcelain seeds germinated (germinative number) 〉
| The Aeschna melanictera porcelain | ?????10m | ??????20m | ?????30m | ??????40m | ??????50m | |||||||
| ??3 | ??5 | ??3 | ??5 | ??3 | ??5 | ??3 | ??5 | ??3 | ??5 | ??3 | ??5 | |
| Rice | ??51 | ??90 | ??45 | ??85 | ??46 | ??91 | ??49 | ??92 | ??40 | ??92 | ??41 | ??88 |
| Radix Raphani | ??90 | ??96 | ??88 | ??95 | ??88 | ??94 | ??89 | ??95 | ??81 | ??94 | ??82 | ??95 |
| Caulis et Folium Chrysanthemi segeti | ??54 | ??70 | ??51 | ??58 | ??50 | ??71 | ??49 | ??69 | ??45 | ??69 | ??46 | ??70 |
| Semen Pisi sativi | ??25 | ??60 | ??24 | ??61 | ??24 | ??58 | ??23 | ??60 | ??20 | ??59 | ??19 | ??58 |
| Caulis et Folium Brassicae capitatae | ??74 | ??93 | ??72 | ??95 | ??74 | ??96 | ??71 | ??94 | ??69 | ??94 | ??68 | ??93 |
The abnormal smells from the patient that the result hints out Aeschna melanictera water in about 30m, exert one's influence and accelerated above germination cycle of listed seed.
Embodiment 13
The influence that＜Aeschna melanictera porcelain and supernatant Aeschna melanictera water germinate to Seem Lablab Album 〉
In order to study the influence of Aeschna melanictera porcelain and supernatant Aeschna melanictera water, carry out the experiment of taking root of Seem Lablab Album seedling and Bulbus Allii Cepae to the growth of plant.
Per 30 Seem Lablab Albums that 1. will be used for cultivating into hyacinth bean sprout are placed on the Schale dish, at room temperature relatively supply with supernatant Aeschna melanictera water, the germination percentage of subsoil water and tap water and rate of growth then in 10 days.
Per 30 Seem Lablab Albums that 2. will be used for cultivating into hyacinth bean sprout are placed on the Aeschna melanictera porcelain, on common porcelain and the plastic containers, at room temperature relatively supply with supernatant Aeschna melanictera water, the germination percentage of subsoil water and tap water and rate of growth then in 10 days.
3. specify 3 Bulbus Allii Cepaes of identical size, supply with supernatant Aeschna melanictera water, the experiment of taking root of subsoil water and tap water.
4. Aeschna melanictera porcelain and supernatant Aeschna melanictera water container coil at a distance of 50m with the Schale that does not have the Aeschna melanictera material.
Below experimental result is described in.
1) growth of the Seem Lablab Album of usefulness Aeschna melanictera porcelain
Because the abnormal smells from the patient of Aeschna melanictera porcelain has been accelerated the germination cycle, has confirmed that the abnormal smells from the patient of Aeschna melanictera porcelain can influence the growth of hyacinth bean sprout.
2) growth of the Seem Lablab Album of usefulness supernatant Aeschna melanictera water
Because the abnormal smells from the patient of Aeschna melanictera water has been accelerated the germination cycle, has confirmed that the abnormal smells from the patient of Aeschna melanictera water can influence the growth of hyacinth bean sprout.
The comparison of the freshness of the plant of＜usefulness Aeschna melanictera porcelain and supernatant Aeschna melanictera water 〉
1. the flower of identical type is put in Aeschna melanictera porcelain and the common porcelain, when supplying with subsoil water, their freshness relatively.
2. the flower of identical type is put in the glass teacup, when supplying with supernatant Aeschna melanictera water, subsoil water and tap water, compared their freshness.
3. Aeschna melanictera porcelain and supernatant Aeschna melanictera water container coil at a distance of 50m with the Schale that does not have the Aeschna melanictera material.
4. employed flower is the Flos Rosae Rugosae of plucking the same time, and each 5 of carnation and Flos Chrysanthemis select the degree of blooming to observe once with bore hole the identical every day of spending.
Below the result is described in
1) freshness of Aeschna melanictera porcelain and plant
Judge that the abnormal smells from the patient of Aeschna melanictera porcelain influences the freshness of plant slightly.
2) freshness of Aeschna melanictera water and plant
Judge that the abnormal smells from the patient of Aeschna melanictera water influences the freshness of plant slightly.
1. respectively 3 planting seeds with Kidney bean, Semen Pisi sativi, Radix Raphani and Caulis et Folium Brassicae capitatae come the rate of growth of comparison plant in the jar that 50g nephrite Aeschna melanictera powder and 50g fine sand are housed.
2. because the fertilising difficulty is not given Radix Raphani and cabbage fertilizing.Yet in order to the rotted leaf of identical mixed and the seed of sand sowing Kidney bean and Semen Pisi sativi.
The jar that 3. the nephrite Aeschna melanictera be housed and common jar be mutual edge distance 50m mutually.
Below the result is described in.
1) growth of Semen Pisi sativi
Because the abnormal smells from the patient of nephrite Aeschna melanictera powder has been accelerated the germination period of Semen Pisi sativi, judges that it influences the growth of Semen Pisi sativi slightly.
2) growth of Kidney bean
Because the abnormal smells from the patient of nephrite Aeschna melanictera powder has been accelerated the germination period of Kidney bean, judges that it influences the growth of Kidney bean slightly.
3) breed of Bulbus Allii Cepae
The abnormal smells from the patient of judging the Aeschna melanictera powder greatly influences taking root of Bulbus Allii Cepae.
4) growth of Radix Raphani
Judge that nephrite Aeschna melanictera powder influences the germination cycle of Radix Raphani, promotes the growth of Radix Raphani.
As what confirmed among the above-described embodiment, can draw following conclusion.
1. the nephrite Aeschna melanictera that obtains from Korea S spring river has the negative value of δ 180, it contains 6～6.5 hardness, the δ D value of measured nephrite Aeschna melanictera is controlled by oh group, the δ 18D value of oh group is all more much lower than this value of all mineral, outside the scope of water, it is δ D (%)=0～-70 on the turn.
2. the component of nephrite jade sample is different with the difference of sampling position.
3. the abnormal smells from the patient that sends from nephrite Aeschna melanictera and Aeschna melanictera porcelain can prolong about 30m to the germination of plant and the influence of growth.
4. the abnormal smells from the patient of Aeschna melanictera water can prolong about 20～30m to the germination of plant and the influence of growth, and its influence than the abnormal smells from the patient of nephrite Aeschna melanictera is weak a little.
5. the experiment of germinateing shows that although there is very little difference between matched group and test group, Aeschna melanictera water and Aeschna melanictera porcelain all promote the germination of seed.
6. nephrite Aeschna melanictera powder has promoted the growth of Radix Raphani, Semen Pisi sativi, Kidney bean and Caulis et Folium Brassicae capitatae, and it hints out the acidify that can enough improve water by a kind of new fertilising of using nephrite Aeschna melanictera and Aeschna melanictera water in breed.Nephrite Aeschna melanictera or Aeschna melanictera water also can be as a kind of nertralizers of drinking water, and the promoter of removing an agent or a plant growth that a kind of concentrated heavy metal is gone is used.
Below experiment (embodiment 16) be in order to study the influence of nephrite Aeschna melanictera product (Aeschna melanictera brick bat, Aeschna melanictera dish, Aeschna melanictera powder, Aeschna melanictera filler or the like) that nephrite Aeschna melanictera by collecting from an Aeschna melanictera ore deposit that the is positioned at Korea S spring river nephrite jadeite ore of jadeite extracting agent of the present invention (as be used for) produces to Marine microorganism.
(1) source of microorganism
In this experiment, sea water obtains from Taejon, Korea, and it is mainly used to produce medium, uses this medium as in the source of marine aging marine bacteria in the stage, wherein most Organic substances is decomposed in 6 months owing to sea water is preserved in the place of dark.The ocean photobacteria of in experiment, using, promptly the fluorescence radiation antibacterial is a kind of gram-negative antibacterial, the luminous density of using sample wherein to show is every milliliter of 1.4 * 1014 light quantum/second.Diluted sample and take out about 1% dilute sample and be used for transplanting.Then, spectrogrph (Milton-Roy MR 3000) and photometer measurement 1ml sample absorption and the luminous density at 660nm used in the cell growth in the research All Time scope and the variation of luminous density respectively.
(2) medium of Shi Yonging
The medium that uses comprises Zobell medium (using it to cultivate marine bacteria traditionally) and the whole media of sea water and their compositions as follows.
＜Zobell medium 〉
Tryptone antibacterial 3g
Yeast extract lg
FeCl
3?????????????????????????????0.1g
Aged sea water 700ml
Distilled water 300ml
The whole media of＜sea water 〉
Tryptone antibacterial 5g
Yeast extract 3g
Glycerol 3ml
Aged sea water 750ml
Distilled water 250ml
Pei Shi culture membrane 3M
The Pei Shi culture membrane that is used for colony formation unit (CFU) as medium obtains from 3M Innovation Properties Company in commercial sources.Number goes out the number of red colony and takes pictures for this colony with digital camera (Kodak DC-120) then.
The Aeschna melanictera brick bat that in experiment, uses, Aeschna melanictera dish, Aeschna melanictera powder, jadeite ore, the sample that Aeschna melanictera filler or the like is made by the nephrite Aeschna melanictera that obtains from Korea S spring river (by Korea S Ocksanga Co., Ltd. produces).After cultivating in the culture bottle of 100ml, relevant experimental group is assigned to each experiment.It is 1g that the sea water of every 100ml uses Aeschna melanictera powder and the employed jadeite ore of 1g.The Aeschna melanictera filler that uses in total weight is 1g.
(3) experiment content
A. the variation of pH
Matched group uses the sea water of 100ml and adds the Aeschna melanictera powder of 1g therein.Then, mixture was stirred 30 minutes, carry out centrifugalize to obtain supernatant Aeschna melanictera water with magnetic stirring apparatus.Then, the variation of the pH of the measurement of concetration supernatant Aeschna melanictera water by the Aeschna melanictera powder.
B. the variation of colony counts
With re-using behind the common sea water of the sea water dilution that does not have microorganism
(solid dielectric 〉
The Pei Shi culture membrane
The Zobell plate
(liquid medium 〉
On the Aeschna melanictera brick bat, cultivate
On the Aeschna melanictera dish, cultivate
Cultivate with jadeite ore
C. the variation of luminous density
Use the sample of fluorescence radiation antibacterial in the whole media of sea water, its luminous density of showing is every milliliter of 1.4 * 1014 light quantum/second.Diluted sample and take out about 1% dilute sample and be used for transplanting.Then, spectrogrph (Milton-Roy MR 3000) and photometer measurement 1ml sample absorption and the luminous density at 660nm used in the cell growth in the research All Time scope and the variation of luminous density respectively.
(4) measurement of Biomass
Use the plain illuminator of Lampyridea luciferase to measure Biomass
Measure Biomass by many diverse ways.Yet, the method for measurement ATP (5-Adenosine triphosphate acid anhydride) quantity, ATP is a kind of biological indicator, because it can obtain in all organisms, it has been used for measuring the Biomass of invisible microorganism.ATP is the genetic factor that is used for keeping than necessity of the more high-grade organism life of antibacterial, and its metabolism by different types of organism produces and uses as a kind of direct energy in cell.Because ATP can promptly be broken down into ADP (5-Adenosine diphosphate acid anhydride) in the organism of death, it is a kind of good indicator of measuring the Biomass of organism alive.On the other hand, if estimate Biomass by bore hole or microscope, under the situation of microorganism, it is very difficult distinguishing alive and dead organism.Under the situation of microorganism, can not use cultural method to measure Biomass visible but (VBNC) cell that can not cultivate.Being less than 1% Marine microorganism also can only utilize common cultural method to cultivate.
Because in the sample that the destruction speed of the organism of living on the surface of Aeschna melanictera product is used in experiment of the present invention is very important, is very important so accurately measure Biomass.Utilize a kind of buffer solution to carry out the measurement of ATP by extracting silt., different chromatographic processes be can use herein, high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) comprised.Under study for action, use a kind of Lampyridea that can utilize ATP to produce light to carry out noctilcent method.Although the method for HPLC or TLC provides the precision of measurement result, The pretreatment also is very complicated, needs a large amount of time and efforts, owing to use UV Absorption or to the dependency of pigment, its accuracy of detection is low.When the oxygen of ATP that uses 1M and 1M was transformed into oxyluciferin to fluorescein, LUC Photinus pyralis LUC Photinus pyralis FL was sent the light of 530nm, and luminous efficiency comes down to 100%, is the highest in the luminescent system that all have been known.The quantity of using LUC Photinus pyralis LUC Photinus pyralis FL to measure ATO is the most sensitive method except that radiosiotope of generally acknowledging.
(5) experimental result
5.1.pH variation
Sea water shows the pH of weak alkalescence usually, as about 8.1, and when it is intactly placed for a long time, can be because the metabolic activity of the microorganism that contains in the sea water and become neutrality gradually.It is very clear and definite that this describes in the matched group of table 21.In the presence of the Aeschna melanictera product, it is added the variation that small pH took place in the back in 1 hour.Aeschna melanictera powder and jadeite ore all show the variation of pH, are similar to 7.8, i.e. the alkalescence level.This trend alleviates under the situation of the Aeschna melanictera brick bat that does not have directly to contact with sea water solution, and is promptly similar to matched group in fact.The result hints out from the elution of Aeschna melanictera product own and goes out material to the environment solution, representational silicon dioxide (SiO
2), changed acidity.
Table 25＜the change pH of sea water〉by the Aeschna melanictera product
| 1 | 2 | 3 hours | |
| Matched group | ????8.18 | ????8.16 | ????8.15 |
| The Aeschna melanictera powder | ????8.01 | ????7.75 | ????7.55 |
| The Aeschna melanictera filler | ????8.10 | ????7.80 | ????7.64 |
| The Aeschna melanictera brick bat | ????8.12 | ????8.05 | ????7.95 |
| The Aeschna melanictera dish | ????8.1 | ????8.0 | ????7.8 |
| Jadeite ore | ????8.1 | ????8.0 | ????7.8 |
5.2. the variation of colony counts
In an aseptic bottle, put into the Copenhagen water of 100ml, and will add in it to the different Aeschna melanictera product of determined number at every turn.Shake the bottle that contains the Aeschna melanictera product with the speed of about 100rmp and come artificial culture, take out 1ml at every turn, transplant with a slice Pei Shi culture membrane then.Cultivate the colony counts (seeing Table 26) that number goes out to produce after 3 days.
Table 26
＜change colony counts by the Aeschna melanictera product 〉
| 1 | 2 | 3 | 7 days | |
| Matched group | ??324 | ??336 | ??328 | ??319 |
| The Aeschna melanictera powder | ??284 | ??272 | ??243 | ??217 |
| The Aeschna melanictera filler | ??274 | ??265 | ??232 | ??204 |
| The Aeschna melanictera brick bat | ??283 | ??274 | ??281 | ??295 |
| The Aeschna melanictera dish | ??272 | ??261 | ??255 | ??275 |
| Jadeite ore | ??269 | ??261 | ??253 | ??282 |
Find that from table 26 all Aeschna melanictera products all show marine bacteria and quantitatively significantly reduce.In early days, the minimizing speed of Aeschna melanictera filler and jadeite ore is very high.After 7 days the cultivation, the minimizing speed of Aeschna melanictera powder and Aeschna melanictera filler is the highest.The Aeschna melanictera filler has the highest minimizing effect especially, and approximate after 7 days is 64% of matched group.
5.3. the variation of luminous density
The Aeschna melanictera product that utilization is used in experiment, the luminous density of fluorescence radiation antibacterial does not have change clearly.Although its change weak to 10% or still less, in all samples, all demonstrate the trend of minimizing.This hints out that the Aeschna melanictera product has suppressed the activity of marine bacteria, shown in above-mentioned experiment.The fluorescence radiation antibacterial is a kind of typically in conjunction with antibacterial, and it is easy to be attached on the Organic substance that occurs in the sea water, and breeds to send fluorescence, and it has increased the probability (seeing Table 27) that together penetrates into fish with bait.
Table 27
＜change the luminous density of fluorescence radiation antibacterial by the Aeschna melanictera product 〉
| 1 | 2 hours | 24 hours | |
| Matched group | ????100 | ????100 | ????100 |
| The Aeschna melanictera powder | ????94 | ????93 | ????88 |
| The Aeschna melanictera filler | ????92 | ????90 | ????85 |
| The Aeschna melanictera brick bat | ????95 | ????92 | ????84 |
| The Aeschna melanictera dish | ????95 | ????90 | ????82 |
| Jadeite ore | ????93 | ????90 | ????82 |
5.4. tack
Antibacterial in the known sea water is attached to the surface that is immersed in the Aeschna melanictera product in the sea water at first, form a kind of biomembrane, and other Marine microorganism is subsequently by attached to it as the biomembranous motion of basal plane material, form biofouling, where do not originate from its antibacterial functions of susceptible of proof from the foregoing description although make the antibacterial functions of Aeschna melanictera product yet clear.Therefore, the Aeschna melanictera product that uses in this experiment can suppress biomembranous formation as a kind of valuable material.
Experimental result shows that bacterial adhesion is 92～95% of a matched group, has showed 5～8% adhere to and reduce effect (seeing Table 28).
Table 28
＜Marine microorganism is in the tack (cultivating for 1 week) of Aeschna melanictera product surface 〉
| The number of bacteria of adhering to | Number * 10 5 | |
| Matched group | ????100 | ????3.2 |
| The Aeschna melanictera brick bat | ????95 | ????3.04 |
| The Aeschna melanictera dish | ????93 | ????2.97 |
| Jadeite ore | ????92 | ????2.94 |
(4) result.
From the foregoing description as seen, the use of the nephrite Aeschna melanictera of jadeite extracting agent of the present invention has reduced 10～40% of common bacteria in the sea water, and it hints out that this experiment value must attempt at the incubator of pilot-scale.Especially, can use the base material of Aeschna melanictera brick bat as incubator, can be at one 2 meters wide, carry out the initial survival rate experiment of fry in 3 meters long and the 1 meter high casing.
Embodiment 17
In order to study the influence of the nephrite Aeschna melanictera that uses in the present invention,, carry out this experiment in the bioinformation system engineering experiment chamber of Korea S Inha university according to applicant's requirement.
Nephrite Aeschna melanictera powder biological engineering result of experiment will be described now.Because what human body was formed 70% is water, between the influence of the change of water and human body, may there be many associations.So research nephrite Aeschna melanictera is to the influence of hard water and the breeding of Folium Digitalis Lanatae plant cell.
A. the variation of hard water
＜experiment 1 〉
Synthetical 50ml hard water (hardness: 100ppm) transfer to respectively in 4 flasks, the nephrite Aeschna melanictera put in wherein two flasks, kept 10 minutes,
Use EDTA (equivalent conversion factor: 2.9412) carry out the variation that hardness is measured in titration.Use buffer solution (pH10) 1ml and EBT as indicator.
The amount of the EDTA that moment consumed that color by hard water is changed by EDTA is measured the variation of hardness.At this moment, As time goes on color may return to original color, but in the present invention, the place measures at the change point.
＜result 1 〉
For the hard water of 100ppm, use the EDTA of 1.70ml.
After the processing of nephrite Aeschna melanictera, use the EDTA of 1.25ml.Therefore, hardness reduces to 73.25ppm from 100ppm.That is to say that the reduction effect of hardness is 26.47%.
＜experiment 2 〉
The 200ml hard water of preparation in experiment 1 (hardness: 100ppm) transfer in 1 flask, the nephrite Aeschna melanictera is immersed wherein.
After 30 minutes, the moisture of filling flask is contained in 3 flasks, measures the variation of hardness.In this experiment, drip EDTA after the color of hard water is changed by EDTA, do not have the change of more color, so that carry out more accurate experiment and measure that hardness.
＜result 2 〉
3 hard water samples that are 100ppm with the preceding hardness of nephrite Aeschna melanictera processing become 89.62ppm respectively, 91.19ppm, and 89.62ppm, and it on average is 90.14ppm.Therefore, the reduction effect of hardness is 9.9%.
＜experiment 3 〉
This experiment is fed water with common pipe and is finished.Be contained in 6 flasks being contained in the pipe moisture in the container, the nephrite Aeschna melanictera be put into the bottom of 3 flasks wherein.Measure hardness after 5 minutes.
＜result 3 〉
3 pipes are given the hardness of water sample, all are 97.48ppm before nephrite Aeschna melanictera of no use is handled, and all are 91.19ppm with the hardness that the nephrite Aeschna melanictera is handled back 3 pipe water samples.Therefore, the reduction effect of hardness is 6.5%.
B. the nephrite Aeschna melanictera is to the influence of Folium Digitalis Lanatae plant suspension cell breeding
The nephrite Aeschna melanictera is to the influence of the Folium Digitalis Lanatae cell proliferation in somatomedin
Figure 10 is illustrated in the variation of cell proliferation whole volumes of viewed liquid medium after 11 days in the somatomedin, and Figure 11 represents the variation of Folium Digitalis Lanatae cell volume.Because the evaporation of the consumption of component and the medium that takes place has in proportion increased the time that cell is grown in medium, these figure have reflected the change in volume that liquid medium is whole.Especially, the humidity of air and temperature have confidential relation with the evaporation of medium, and these figure also influence the speed of cell growth.
As shown in the figure, the whole volume of liquid medium under the situation of using the nephrite Aeschna melanictera reduces gradually with respect to the matched group that does not use the nephrite Aeschna melanictera.When using the nephrite Aeschna melanictera, the cell speed of growth is opposite with the evaporation of medium, and the consumption rate matched group of nutrient substance is fast, and for this reason, quick minimizing that can the medium that partial offset consumed.As shown in figure 10, only measure the variation of the volume of packaged cell, cultivating 4 days after 11 days, under the situation of using the nephrite Aeschna melanictera continuously, the increase of observing the volume cell surpasses the matched group that does not use the nephrite Aeschna melanictera.Therefore, when using the nephrite Aeschna melanictera, the cell speed of growth is faster when not using the nephrite Aeschna melanictera.These results are understood to, in this experiment, use the quick speed of growth of cell of nephrite Aeschna melanictera to offset because whole medium volumes that the consumption of nutrient substance and medium evaporation cause reduce, this has caused comparing with matched group, will stop the underspeeding of whole volumes of liquid medium.In addition, the definite volume result of cell of contrast breeding has shown the trend that the volume of cell after 4 days increases gradually and also can have been supported above-mentioned conclusion by understanding.
Measure the total weight of cell and the most frequently used index that the dry weight conduct is used for measuring the cell proliferation stage.The growth curve of each cell has been represented sigmoid curve generally and this sigmoid curve has been divided into four-stage: the lag phase, and wherein acellular breeding and do not have quality and increase, this is a pre-adaptive phase to medium; In the index stage, it shows an abrupt slope, because through after the lag phase, reproduction speed is accelerated, quality increases; Quiescent phase, wherein the cell growth reaches maximum, and breeding is prevented from, and no quality increases; With the dead stage, the volume and the quality of wherein all cells all reduce, because because the consumption of nutrient substance, the secretion of toxic component and the saturation of cell density make cell destroyed and dead.
Figure 12 and 13 is the total weight of cell and the measurement result of dry weight.Figure 12 is after above-mentioned four-stage growth curve.The experimental group that uses the nephrite Aeschna melanictera showed high any reproduction speed than the matched group that does not use the nephrite Aeschna melanictera after 7 days, and the former breeding tends to last till the 10th day, and the latter's breeding stops the total weight minimizing with cell.When as the concentration change of the glucose of the metabolism machine matter in a kind of main medium, the concentration that lasts till glucose according to the growth of cell of the present invention in medium is near zero (because consumption of nutrient substance), and (not using the nephrite Aeschna melanictera) stops to breed and work as the speed of growth reduction of nutrient substance cell when exhausting in the medium of contrast.This hint nephrite Aeschna melanictera can influence the growth of cell.This result also shows in Figure 13 of the dry weight of measuring cell.In Figure 13, the cell growth after 3 days in the present invention's's (use nephrite Aeschna melanictera) medium with the cell growth phase ratio that (do not use the nephrite Aeschna melanictera) in the medium of matched group, has been represented reproduction speed faster, and the phenomenon identical with Figure 12 occurred after 9 days.So, cultivate with the approaching somatomedin of nephrite Aeschna melanictera in cell compare with matched group, show the influence of good cell growth.When using the nephrite Aeschna melanictera to cultivate to have the ultimate Folium Digitalis Lanatae plant cell of about 10 days growth, this cell is kept steady statue, wherein just exchanged new medium after 10 days and necrocytosis does not take place, and shows the breeding of cell.Owing to this reason, can be applied to make the optimization of cell growth and concentration as a kind of process of the useful materials of growing in a kind of welcome mode according to method of the present invention.
2. the nephrite Aeschna melanictera is to the influence of the variation of medium pH
After Figure 14 is illustrated in the variation of cellular pH in the culture medium and Figure 15 and represents to add nephrite Aeschna melanictera powder, the variation of cellular pH in the culture medium.
Specifically, Figure 14 measures the variation of pH in the medium that uses the nephrite Aeschna melanictera.The variation tendency and the pH that also occur being applicable to the pH in the medium of typical plant cell in matched group descend gradually in time, remain on certain level then.Yet when using the nephrite Aeschna melanictera, the trend of pH is similar to matched group, and pH just begins to increase gradually after 7 days.This phenomenon appears among Figure 15 significantly, and it measures the variation of pH when directly adding nephrite Aeschna melanictera powder in medium.Do not add the nephrite Aeschna melanictera in the medium in matched group, pH value descends continuously, and when adding the nephrite Aeschna melanictera therein, pH tends to maintain a certain degree.By inference, owing to working, the ionic reaction that is included in the mineral constituent in the nephrite Aeschna melanictera caused above-mentioned result.
3. the nephrite Aeschna melanictera is to the analysis result of the experiment of the quality of water
Figure 16 and 17 shows the condition of analytical tool and the analysis result of output, and the data in Figure 16 and 17 are corresponding to each sample, and the result is illustrated in table 29 to 32.In table 29, add after 48 hours behind the nephrite Aeschna melanictera of a 20g, measure (sample 1) each analytic attribute, if in each analytic attribute, do not change, there is not the influence that brings by the nephrite Aeschna melanictera so.
In table 30, after the nephrite Aeschna melanictera of a 20g taken out 48 hours, measure each analytic attribute of (sample 2) from solution, each analytic attribute does not almost change.
In table 32, from solution, to take out in the analysis experiment of (sample 4) at nephrite Aeschna melanictera a 20g, each analytical characteristics does not change.Yet, in sample 3, the nephrite Aeschna melanictera powder of 20g is put into solution, be illustrated in the table 31 through the measurement data of each analytic attribute after 48 hours.As shown in table 31, exist many variations and these variations as follows.
Harmful Cr, Pb, the quantity of Ni and Co was greatly changed after 48 hours.The quantity of the Cr of (as before reacting) is 45.30mg before the adding nephrite Aeschna melanictera powder, and after 48 hours, it becomes 0 behind the adding nephrite Aeschna melanictera powder.Pb is also fully removed from 13.76mg goes to 0.The quantity of Ni also drops to 11.94mg from 52.69mg from the quantity that 51.8mg drops to 1.733mg and Co.Mg, a kind of composition of needed by human, it is because the result that Mg combines with the component of nephrite Aeschna melanictera self that its quantity is increased to 55.74mg and this from 48.36mg, but hardness does not change.
Hydrogen ion concentration occurs as tart pH3.5 in the distilled water, but changes to neutral pH6.8, and electric conductivity promptly descends.Absorbability is the about 3～4mmol.q of every 1g (equivalent), and it is a very high value in the physical sense.
Table 29
Preceding and the reacted analysis result of＜reaction
(sample 1: be added with nephrite Aeschna melanictera piece (20g) in the solution) 〉
| Project | Before the reaction | Reaction back (48 hours) | Variable quantity |
| ????pH | ????3.5 | ????3.82 | ????- |
| ????Ni | ????51.8mg | ????51.75mg | ????- |
| ????Co | ????52.69mg | ????52.54mg | ????- |
| ????Cr | ????45.30mg | ????43.88mg | ????- |
| ????Mg | ????48.36mg | ????48.59mg | ????- |
| ????Pb | ????13.76mg | ????13.90mg | ????- |
Table 30
Preceding and the reacted analysis result of＜reaction
(sample 2: from solution, take out nephrite Aeschna melanictera piece (20g)) 〉
| Project | Before the reaction | Reaction back (48 hours) | Variable quantity |
| ????pH | ??3.5 | ????3.65 | ????- |
| ????Ni | ??51.8mg | ????48.92mg | ????- |
| ????Co | ??52.69mg | ????49.83mg | ????- |
| ????Cr | ??45.30mg | ????41.23mg | ????- |
| ????Mg | ??48.36mg | ????47.97mg | ????- |
| ????Pb | ??13.76mg | ????15.1mg | ????- |
Table 31
(the preceding and reacted analysis result of reaction
(sample 3: be added with nephrite Aeschna melanictera powder (20g) in the solution) 〉
| Project | Before the reaction | Reaction back (48 hours) | Variable quantity |
| ????pH | ??3.5 | ????6.8 | ??+3.3 |
| ????Ni | ??51.8mg | ????1.733mg | ??-50.06mg |
| ????Co | ??52.69mg | ????11.94mg | ??-40.75mg |
| ????Cr | ??45.30mg | ????0mg | ??-45.30mg |
| ????Mg | ??48.36mg | ????55.74mg | ??+7.38mg |
| ????Pb | ??13.76mg | ????0mg | ??-13.76mg |
Table 32
Preceding and the reacted analysis result of＜reaction
(sample 4: from solution, take out nephrite Aeschna melanictera powder (20g)) 〉
| Project | Before the reaction | Reaction back (48 hours) | Variable quantity |
| ????PH | ??3.5 | ????3.7 | ????- |
| ????Ni | ??51.8mg | ????51.53mg | ????- |
| ????Co | ??52.69mg | ????52.55mg | ????- |
| ????Cr | ??45.30mg | ????43.0mg | ????- |
| ????Mg | ??48.36mg | ????48.39mg | ????- |
| ????Pb | ??13.76mg | ????14.29mg | ????- |
As mentioned above, near the breeding of the Folium Digitalis Lanatae plant suspension cell in 1 week of the artificial culture nephrite Aeschna melanictera is compared with identical but nephrite Aeschna melanictera cultured cells of no use, has increased about 30%.Its result who obtains is very surprising, because result similar with it formerly carries out never taking place with high the cultivation in all various experiments that the Folium Digitalis Lanatae plant cell is bred under the concentration with research.In addition, in the experiment of the variation of hard water, even but find that the nephrite Aeschna melanictera does not contact the still fact of demineralized water with water.Especially, in to the experiment that precipitates the elementary analysis in the distilled water that nephrite Aeschna melanictera powder is arranged, pH takes place to be increased, and Ni and Co reduce, the special result that heavy metal such as Cr, Pb are removed and Mg increases.
Although also do not make the effect of the nephrite Aeschna melanictera (powder) of the present invention's use in theory clear, but its effect (does not change hard water as not contacting water, make the breeding of Folium Digitalis Lanatae plant suspension cell increase by 30%, reduce Ni and Co, remove to the deleterious heavy metal of human body, as Cr and Pb) obtain by the ionic reaction of the inorganic constituents that is contained in electromagnetic release that in the IR result of study, demonstrates and the nephrite Aeschna melanictera proving conclusively.Proved the above-mentioned fact by the effect that is illustrated in the product of the present invention.
This experiment is ought or join from the water that different water sources provides the variation (seeing Figure 18 to 21) of the pH in 8 to 11 days All Time scope jadeite ore, Aeschna melanictera necklace and the submergence of Aeschna melanictera powder in order to study.
＜result 〉
1. when jadeite ore and Aeschna melanictera necklace being immersed into when coming from common water purifier and the phreatic water, the pH level of jadeite ore and necklace (with the nephrite Aeschna melanictera manufacturing of using among the present invention that obtains from the Chunchon, Korea) is higher than what make in China and Russia.
2. 10%, 5% and 1% Aeschna melanictera powder is joined in the strong acid solution of pH3.2, pH value average in 10 days is respectively 8.59 ± 0.10,8.58 ± 0.13 and 8.57 ± 0.11, and the Aeschna melanictera powder that is added has been showed the difference of very little percentage ratio.
3. during measuring, the pH level of handling the strong acid solution in the bottle at Aeschna melanictera is higher than in the conventional bottle always.Shown in above-mentioned discovery, jadeite ore, Aeschna melanictera powder and the Aeschna melanictera necklace of the nephrite Aeschna melanictera manufacturing that obtains from the Chunchon, Korea compared with the product of making in China and Russia, and pH increases, but does not have the difference on the statistical significance.
Embodiment 19
Experiment is the variation of measuring biorhythm with radio biological field analyzer (trade name OMNI-SENSE) in order to study, in each experiment, specify the women adult of 39 years old healthy male adult and 19 years old to wear and be positioned at the macrobead necklace that the nephrite Aeschna melanictera in the Aeschna melanictera ore deposit of Chunchon, Korea makes and take nephrite Aeschna melanictera tea, analyze four kinds of typical situations by collection.Experimental result is described in table 33 and 34.
Table 33
＜Aeschna melanictera necklace (having macrobead) 〉
| Male (40 years old) | Women (19 years old) | |||
| Before the processing | After the processing | Before the processing | After the processing | |
| Angina pectoris (52071) | ??21 | ??43 | ??43 | ??45 |
| Diabetes (11009) | ??32 | ??35 | ??37 | ??54 |
| Hypertension (40520) | ??31 | ??44 | ??45 | ??44 |
| Sciatica (40228) | ??12 | ??24 | ??53 | ??54 |
*The internal measurement number of the numeral radio biological field analyzer (OMNI-SENSE) in the bracket.
Table 34
＜Aeschna melanictera tea 〉
| Male (39 years old) | Women (21 years old) | |||
| Before the processing | After the processing | Before the processing | After the processing | |
| Angina pectoris (52071) | ??53 | ??64 | ??35 | ??44 |
| Diabetes (11009) | ??47 | ??53 | ??42 | ??43 |
| Hypertension (40520) | ??48 | ??57 | ??33 | ??41 |
| Sciatica (40228) | ??43 | ??61 | ??23 | ??52 |
Shown in above-mentioned result, very big difference is arranged with Aeschna melanictera measured value before and after treatment.Higher value means that the Aeschna melanictera component is more useful to human body.Although the adult to health carries out the above embodiments, when being applied to patient, the bigger difference in measured value is observed in expectation.The radio biological field analyzer that this experiment is used is the analyzer that a kind of traditional analog radio biological field analyzer and digital radio biological field analyzer combine.The radio biological field analyzer that this experiment is used uses the shared storehouse of 64K byte.Be used for measuring the speed of about 400 elementary items of the physical qualification of human body, comprise equilibrium between yin and yang, the hormone balance, metabolic problems, vitamin deficiency, mineral deficiency, sense organ or the like all is programmed in the shared storehouse.
Below the reference value of radio biological field analyzer and its measuring condition are summarised in.
The reference value of table 35＜radio biological field analyzer 〉
| ????0-25％ | Relatively poor |
| ????25-45％ | Well |
| ????45-55％ | Very good |
| ????55-75％ | Well |
| ????75-100％ | Relatively poor |
Allowable error: the physical qualification according to the environmental factors of measuring the place and the people who accepts to analyze allows ± 5%
Measuring range :-100～+ 100%, normal in 0～100% scope
Measurement environment: the place of no electromagnetic interference, preferably do not have high-amplitude wave and disturb the place that takes place
Power supply: compatible 220VAC socket and internal cell (A4 * 4)
The medical effect of the different product that contains nephrite Aeschna melanictera powder that embodiment 20 and 21 expressions are used in the present invention.
In order to study beneficial effect,, finish experiment by the Chinese medicine center that is attached to land for growing field crops university as necklace, ring, bracelet by the different product of the ornament that contains nephrite Aeschna melanictera powder of embodiments of the invention preparations.
Wear ornament for the masculinity and femininity adult who surpasses 45 years old, surpass 2 hours taking according to the ornament of the present invention's preparation, the variation of research physical qualification the results are shown in following table 36 in 38.
Table 36＜to the adult test of 45 years old male 〉
| Common ornament | The ornament that contains the nephrite Aeschna melanictera | ||
| ????HR(SaO 2) | ??81BPM | ????76BPM | |
| ??NIBP | Systole | ??127mmHg | ????128mmHg |
| On average | ??107mmHg | ????104mmHg | |
| Relaxing period | ??80mmHg | ????76mmHg | |
| Oxygen concentration (SaO 2) | ??91％ | ????93％ | |
| Pulse | ??81BPM | ????76BPM | |
Table 37＜to the adult test of 55 years old male 〉
| Common ornament | The ornament that contains the nephrite Aeschna melanictera | ||
| ????HR(SaO 2) | ??81BPM | ????85BPM | |
| ??NIBP | Systole | ??181mmHg | ????173mmHg |
| On average | ??142mmHg | ????127mmHg | |
| Relaxing period | ??111mmHg | ????110mmHg | |
| Oxygen concentration (SaO 2) | ??95％ | ????95％ | |
| Pulse | ??83BPM | ????85BPM | |
Table 38＜to the adult test of 64 years old women 〉
| Common ornament | The ornament that contains the nephrite Aeschna melanictera | ||
| ????HR(SaO 2) | ??68BPM | ????68BPM | |
| ??NIBP | Systole | ??185mmHg | ????176mmHg |
| On average | ??117mmHg | ????125mmHg | |
| Relaxing period | ??104mmHg | ????105mmHg | |
| Oxygen concentration (SaO 2) | ??74％ | ????96％ | |
| Pulse | ??68BPM | ????68BPM | |
To the result as shown in 38, the oxygen concentration in the people who wears the ornament of being made by nephrite Aeschna melanictera of the present invention is than the people Geng Gao that wears common ornament as table 36.Therefore, ornament of the present invention has good enhancing metabolism and level and smooth sanguimotor curative effect.
Embodiment 21
The patient who suffers from several diseases is carried out clinical trial, and the bed that patient is used, room, sofa, chair or the like cover the cloth that the nephrite Aeschna melanictera of going up the present invention's use is made.The clinical experiment result proves that 88% patient's disease alleviates.
◎ selects experimental standard and method
In the people of the Chinese medicine center treatment that is attached to land for growing field crops university, select 25 and suffer from headache, insomnia, dizzy, trick discomfort or numb patient carry out clinical trial.
◎ observes attribute and method
Allow to suffer from headache, insomnia, dizzy, the uncomfortable or numb patient of trick keeps certain prediction regular (above 10 days) to ornament, observes patient's physical qualification and variation then.
◎ evaluation criterion and method
A. evaluation criterion: the condition of medical research first as observing attribute
B. evaluation methodology: be described in the following table 39.The result is illustrated in the table 40,
Table 39
| In proper order | Influence | Estimate (%) |
| ????1 | Can ignore or not have influence | ????＜70％ |
| ????2 | Influential | ????≥70％ |
| ????3 | Appreciable impact | ????≥80％ |
| ????4 | Almost completely cure | ????≥90％ |
Table 40
| Disease | There is not influence | Influential | Appreciable impact | Almost completely cure | Amount to |
| ??＜70％ | ??≥70％ | ??≥80％ | ????≥90％ | ||
| Headache or dizzy | ??2 | ??3 | ??5 | ????1 | ??11 |
| Insomnia | ??0 | ??2 | ??1 | ????1 | ??4 |
| Uncomfortable | ??1 | ??1 | ??1 | ????2 | ??5 |
| Head or foot numbness | ??0 | ??1 | ??1 | ????1 | ??3 |
| Dyspepsia | ??0 | ??0 | ??2 | ????0 | ??2 |
| Amount to | ??3(12％) | ??7(28％) | ??10(40％) | ????5(20％) | ??25 ?(100％) |
Shown in the result in the top table 40, specify the headache of the most patient of carrying out clinical trial, insomnia, dizzy, uncomfortable, diseases such as trick discomfort or numbness take a turn for the better to some extent.These results have fully proved the good medical curative effect of nephrite Aeschna melanictera.
Embodiment 22
The contrast teacup that test teacup of being made by the nephrite Aeschna melanictera that uses among the present invention and polyethylene are made is all filled the A level milk of homogenizing, allows it at room temperature to place 48 hours.Then milk is analyzed.The result is illustrated in the following table 41.
Table 41
| Experiment | The contrast teacup | The experiment teacup |
| Escherichia coli | Do not exist/ml | Do not exist/ml |
| Lactobacillus | ????45000cfu/ml | ????37000cfu/ml |
| The quantity of yeast ﹠ mycete | ????310cfu/ml | ????280cfu/ml |
| The quantity that the standard plate is cultivated | Surpass 3 * 10 6cfu/ml | Surpass 3 * 10 6cfu/ml |
Conclusion: reduced harmful microbial numbers.
In the present embodiment, the food decomposition rate of the synthetic resin rice bowl made by the nephrite Aeschna melanictera that uses among the present invention is experimentized, shown in table 42.
Table 42
| Sample | The Aeschna melanictera bowl |
| Outward appearance | The off-white color powder |
| Experimental technique | Describe below |
| The result | Describe below |
Experimental technique: five Aeschna melanictera bowls (experimental group) and five common bowls (matched group) are all filled the rice and the sterilized water of equal volume, allow at room temperature to place 24 hours.Two groups are about 1 meter far away of distance mutually, does not all have the bowl lid and is exposed in the air fully.Shown in table 43, carry out the test that the standard plate is cultivated quantity.
Table 43
| Bowl | Matched | 0 hour | The test group |
| ??#1 | ??0，0 | ???0，0 | |
| ??#2 | ??0，0 | ???0，0 | |
| ??#3 | ??0，0 | ???0，0 | |
| ??#4 | ??0，0 | ???0，0 | |
| ??#5 | ??0，0 | ???0，0 | |
| 18 hours | |||
| ??#1 | ??721，635 | ???666，690 | |
| ??#2 | ??516，608 | ???522，620 | |
| ??#3 | ??629，715 | ???570，620 | |
| ??#4 | ??777，595 | ???707，724 | |
| ??#5 | ??737，701 | ???731，678 | |
| 24 hours | |||
| ??#1 | ??1036，981 | ???1005，890 | |
| ??#2 | ??1210，1281 | ???1060，1095 | |
| ??#3 | ??1068，889 | ???951，180 | |
| ??#4 | ??972，1050 | ???1002，971 | |
| ??#5 | ??1042，1160 | ???978，1149 | |
Conclusion: in test group bowl and in the decomposition of the food in the matched group bowl, the food decomposition rate of test group bowl is lower than matched group bowl, and this shows that Aeschna melanictera of the present invention has favorable influence to the storage of food.
Embodiment 23
The Aeschna melanictera bowl that this experimentation is made by the nephrite Aeschna melanictera that uses among the present invention is to the influence of Carnis Sus domestica freshness.Details are described in the table 44.
Table 44
| Temperature (℃) | ????????????????0 | ?????????????????4 | |||
| Container | Contrast | Aeschna melanictera | Contrast | Aeschna melanictera | |
| 0 day | ??PH | ???????????????????????????????5.83±0.04 | |||
| ??VBN | ???????????????????????????????3.50±0.43 | ||||
| ??TBA | ???????????????????????????????0.063±0.013 | ||||
| Color (Δ E) | ???????????????????????????????53.70±4.34 | ||||
| 4 days | Juice (%) when thawing | ??0.14 | ??0.57 | ??0.54 | ??0.01 |
| ??PH | ??5.46±0.04 | ??5.42±0.00 | ??5.59±0.01 | ??5.69±0.01 | |
| ??VBN | ??5.56±0.20 | ??4.29±0.20 | ??5.56±0.20 | ??5.42±1.41 | |
| ??TBA | ??0.153±0.20 | ??0.104±0.20 | ??0.122±0006 | ??0.099±0.025 | |
| Color (Δ E) | ??55.80±0.01 | ??49.30±0.11 | ??54.90±0.08 | ??56.50±0.07 | |
| 7 days | Juice (%) when thawing | ??1.02 | ??0.17 | ??1.90 | ??0.43 |
| ??pH | ??5.77±0.03 | ??5.84±0.01 | ??5.56±0.01 | ??5.65±0.01 | |
| ??VBN | ??2.36±0.00 | ??2.32±0.00 | ??3.48±0.45 | ??2.59±0.22 | |
| ??TBA | ??0.234±0.013 | ??0.203±0.006 | ??52.80±0.06 | ??52.90±0.05 | |
| Color (Δ E) | ??55.50±0.47 | ??52.60±0.75 | ??52.80±0.06 | ??52.90±0.05 | |
| 14 days | Juice (%) when thawing | ??0.92 | ??0.27 | ??2.70 | ??0.19 |
| ??PH | ??5.50±0.01 | ??5.67±0.03 | ??6.71±0.00 | ??5.91±0.02 | |
| ??VBN | ??3.00±0.39 | ??3.98±0.18 | ??6.17±0.87 | ??3.85±0.53 | |
| ??TBA | ??0.162±0 | ??0.176±0.019 | ??2.406±0.191 | ??0.811±0.089 | |
| Color (Δ E) | ??51.70±0.10 | ??53.80±0.13 | ??62.80±0.00 | ??56.70±0.04 | |
| 21 days | Juice (%) when thawing | ??1.11 | ??0.35 | ??0.85 | ??0.25 |
| ??PH | ??6.41±0.01 | ??6.07±0.00 | ??7.24±0.00 | ??6.69±0.01 | |
| ??VBN | ??5.32±0.36 | ??7.35±0.42 | ??30.67±3.31 | ??15.08±0.98 | |
| ??TBA | ??10.220±0.230 | ??6.852±0.274 | ??10.15±0.198 | ??2.298±0.102 | |
| Color (Δ E) | ??52.40±0.06 | ??59.20±0.13 | ??58.20±0.13 | ??66.30±0.33 | |
Juice a: % when thawing
VBN：mg％
TBA:mgMal/Kg meat
Color: yellowish pink
When being kept at Carnis Sus domestica in the Aeschna melanictera bowl of being made by the nephrite Aeschna melanictera that uses among the present invention (Chuncheon, Korea S), the nephrite Aeschna melanictera is to the influence of the variation of pork freshness in the regular hour scope in order to research in this experiment.0,4, in 7,14,21 days, perhaps Carnis Sus domestica is kept in the Aeschna melanictera bowl, perhaps Carnis Sus domestica is kept in the common bowl (contrast).Protein-rotten) and the oxidation (TBA) of lipids in each container, preserve the Carnis Sus domestica that a hectogram (100g) is downcut from the Petaso position, measure the juice that drips when thawing, pH, evaporable basic nitrogen (VBN:.
＜result 〉
Drip juice still less when 1. being kept at the thawing of Carnis Sus domestica in the Aeschna melanictera bowl, and As time goes on be tending towards a large amount of minimizings, irrelevant with storage temperature.
2. as the dead indicator that changes of a kind of muscle, although the not change that produces tangible pH because of the kind of container is higher at 0 ℃ at 4 ℃ pH ratio.
3. the VBN value of indicator protein qualitative change matter degree, at 0 ℃, Carnis Sus domestica quite high and that be kept in the time of 4 ℃ in the Aeschna melanictera bowl is As time goes on obviously very low when preserving 14 days and 21 days.
4. the VBN value of indication lipids degree of oxidation that is kept at the Carnis Sus domestica in the Aeschna melanictera bowl is lower always, and is irrelevant with storage temperature.
Embodiment 24
When this experimentation is contained coffee when the Aeschna melanictera cup made from the nephrite Aeschna melanictera that uses in by the present invention, may change the effect of Aeschna melanictera cup of the mouthfeel of coffee.Food and nutrition branch by Chung-Ang university finish experiment.Below details are described in.
1. experiment purpose
In order to study when coffee being contained in Aeschna melanictera cup or common cup (contrast) lining, coffee is at taste, fragrance, pleasant impression, and/or change in color.
2. experimental technique: paired hobby property testing
Sample of ■ test is than the preference of another sample
■ tests attribute: taste, fragrance, pleasant impression, color
3. sensation group
The food of Korea S Chung-Ang university and 20～25 final-year students of nutrition specialty (it lives through subjective appreciation, has the knowledge of this method of testing)
4. experimental technique
■ March is to June, 1996
■ is weekly
■ amounts to 10 times
5. experiment container and beverage types
■ Aeschna melanictera cup compares with common cup: all have identical outward appearance
■ beverage: instant coffee
6. the preparation of sample
A coffee is blended in the 1/2 teacup hot water
7. add up: T-tests (p≤0.05)
＜result 〉
1. mouthfeel: be contained in coffee in the Aeschna melanictera cup and produce still less bitterness (p≤0.05).
2. color and pleasant impression: the Aeschna melanictera cup tends to provide better color and pleasant impression, but does not have statistical significance.
3. fragrance: common cup provides better coffee aroma than Aeschna melanictera cup.
(conclusion 〉
As if the Aeschna melanictera cup can make the taste of coffee lighter (confirming that it has statistical significance).Be contained in the pleasant impression and color and similar or better (the no statistical significance) that is contained in common glass of the coffee in the Aeschna melanictera cup.
The result shows nephrite Aeschna melanictera retort solution of the present invention, when being used to drink with medical treatment, has the metabolic effect of the medical function and the promotion human body of treatment disease.
As mentioned above, according to the present invention, the nephrite jadeite extracting agent can obtain from the nephrite jadeite ore that nephrite Aeschna melanictera ore deposit collects, and it has the good medical function of prevention as diseases such as headache, insomnia, feeling of numbness, dyspepsias by blood circulation promoting and metabolism.
Claims (6)
1. jadeite extracting agent that is used for making nephrite Aeschna melanictera retort solution, it makes as follows: the nephrite jadeite ore carries out preprocessing by pulverizing, selection and cleaning, and in vaporizer, mix with 1: 4 mixed proportion with sterile distilled water, heating blends under 100 ℃ high temperature or higher temperature, the component of elution enters into the distilled water that boils and boil from jadeite ore, with distilled water it is evaporated, steam is transformed into distillate by heat exchange.
2. jadeite extracting agent as claimed in claim 1, wherein jadeite ore is σ
18The cryptocrystal tremolite of O negative value, its contained component is as follows:
The semi-quantitative analysis (%) of nephrite Aeschna melanictera powder
Silicon 34 stannum 0.024
Magnesium 10 berylliums 0.00072
Calcium 4.9 silver medals 0.0013
Ferrum 0.23 titanium 0.0038
Aluminum 0.16 nickel 0.0028
Copper 0.17 chromium 0.0030
Cobalt 0.046 other element 0
Manganese 0.14
3. jadeite extracting agent as claimed in claim 1 wherein joins minor amounts of additives and pigment or other coloring agent in the jadeite extracting agent, and at health care (motion) ion beverage, the dietary fiber beverage uses in nutritional supplement and other the soda pop with it.
4. method for preparing jadeite extracting agent, the step that it comprises:
Mineral powder is broken into predetermined size, it is chosen and cleans to finish the preprocessing step;
The preparation distilled water:
The jadeite ore and the distilled water that in a vaporizer, mix preprocessing with 1: 4 mixed proportion, heating blends under 100 ℃ high temperature or higher temperature uses distilled water evaporation elution to advance jadeite ore material in the distilled water; With
By heat exchange the steam that contains the jadeite ore material that produces in evaporation step is transformed into distillate.
5. method as claimed in claim 4, wherein jadeite ore is σ
18The cryptocrystal tremolite of O negative value, its contained component is as follows:
The semi-quantitative analysis (%) of nephrite Aeschna melanictera powder
Silicon 34 stannum 0.024
Magnesium 10 berylliums 0.00072
Calcium 4.9 silver medals 0.0013
Ferrum 0.23 titanium 0.0038
Aluminum 0.16 nickel 0.0028
Copper 0.17 chromium 0.0030
Cobalt 0.046 other element 0
Manganese 0.14
6. method as claimed in claim 4 wherein joins minor amounts of additives and pigment or other coloring agent in the jadeite extracting agent, and at health care (motion) ion beverage, the dietary fiber beverage uses in nutritional supplement and other the soda pop with it.
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|---|---|---|---|
| CN00127073A CN1343495A (en) | 2000-09-15 | 2000-09-15 | Jadeite extracting agent and its prepn. method |
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|---|---|---|---|
| CN00127073A CN1343495A (en) | 2000-09-15 | 2000-09-15 | Jadeite extracting agent and its prepn. method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060515A (en) * | 2010-11-17 | 2011-05-18 | 昆明理工大学 | Process for preparing synthetic jadeite |
| CN102119915A (en) * | 2011-03-01 | 2011-07-13 | 刘东才 | Method for making skin cream by using jade powder |
-
2000
- 2000-09-15 CN CN00127073A patent/CN1343495A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060515A (en) * | 2010-11-17 | 2011-05-18 | 昆明理工大学 | Process for preparing synthetic jadeite |
| CN102060515B (en) * | 2010-11-17 | 2013-03-06 | 昆明理工大学 | Process for preparing synthetic jadeite |
| CN102119915A (en) * | 2011-03-01 | 2011-07-13 | 刘东才 | Method for making skin cream by using jade powder |
| CN102119915B (en) * | 2011-03-01 | 2012-07-25 | 刘东才 | Method for making skin cream by using jade powder |
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