CN1329518C - Method for producing 1,3-propylene glycol through ferment in high cell density by using bacteria in intestinal tract - Google Patents
Method for producing 1,3-propylene glycol through ferment in high cell density by using bacteria in intestinal tract Download PDFInfo
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- CN1329518C CN1329518C CNB2005100387246A CN200510038724A CN1329518C CN 1329518 C CN1329518 C CN 1329518C CN B2005100387246 A CNB2005100387246 A CN B2005100387246A CN 200510038724 A CN200510038724 A CN 200510038724A CN 1329518 C CN1329518 C CN 1329518C
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Abstract
The present invention relates to a method for fermenting and producing 1, 3-propanediol by the high cell density of enterocin, particularly to a method for preparing 1, 3-propanediol through biologic fermentation. The technological process comprises: in strain culture, strains of enterocin are inoculated in sterile strain culture liquid after activated; in the culture of fermentation culture liquid, strain liquid is inoculated in a fermentation tank containing fermentation culture liquid with the inoculum size with the volume ratio of 3 to 15% after fermentation culture fluid is disinfected and cooled; in the quick growth period of batch fermentation thallus, the concentration of carbon sources in fermentation culture liquid is stabilized between 0.1 to 30 g/L; in the fermentation period of high cell density, the density of thallus reaches OD <650> 2.0 to 5.0. The modes that nutrition substrates are restrictively supplied, and air is restrictively fed are adopted. When products are synthesized, one kind or the combination of a plurality of kinds in nitrogen sources, phosphates, inorganic ions, trace elements and V<B12> which can be utilized by the growth and the conversion of thallus are supplied to the fermentation tank simultaneously, and glycerol is added simultaneously. When acid is not produced in fermentation, fermentation is finished.
Description
Technical field
The present invention is a kind of biological fermentation preparation 1, and the method for ammediol belongs to 1, the biocatalysis synthesis technical field of ammediol.
Background technology
1, ammediol is a kind of important organic synthesis raw material and intermediate, has widely to use.1, ammediol can be used for the synthetic of softening agent, washing composition, sanitas, emulsifying agent; Also be used for industries such as food, makeup and pharmacy.It can generate urethane with isocyanate reaction, is the important monomer of producing urethane; Particularly 1, ammediol can with terephthaldehyde's acid-respons, aggregate into the PTT trevira, this new polyester material not only has the biodegradable characteristic of the natural circulation of being easy to, and has excellent rebound resilience, dyeability, pollution resistance etc.Fully developing talents in Application Areass such as carpet, engineering plastics, dress materials, is the focus of present synthon new varieties.Though just synthesize successfully as far back as nineteen forty-one PTT, expensive raw material 1, ammediol has hindered its development.According to the prediction of Schell company, will be to world's ptt fiber demand in 2010 above 1,000,000 tons, when the time comes, to 1, the demand of ammediol also rises straight line.
Present 1, the production method of ammediol is divided into following several: the epoxyethane method of (1) Shell company exploitation, this method is raw material with oxyethane, under the effect of catalyzer with CO prepared in reaction 3-hydroxy propanal, the 3-hydroxy propanal is shortening system 1 under the Ni catalyst action, ammediol.(2) the propenal method of Degussa company exploitation, this method is raw material with the propenal, carries out two key hydrations with water and make the 3-hydroxy propanal under the effect of an acidic catalyst or ion-exchanger, is converted into 1 again, ammediol.(3) biological fermentation process: under the microorganism catalysis effect, the fermentability carbon source through fermentation is converted into 1, ammediol.(4) other method: inquiring into conversion of ethylene as Japanese Supreme Being people company is 1, the technological line of ammediol, and the chemical process of this technological line is complicated, but raw materials used ethene is cheap and easy to get.
Above-mentioned be used for syntheticly 1, the chemical process of ammediol all is not suitable for industrial mass production 1, ammediol, and the chemical process production cost is higher, and technical difficulty height, particularly Preparation of catalysts be difficulty.Chemical method has not only consumed non-renewable limited resources simultaneously, and can pollute environment.And fermentation method to utilize starch, glucose or glycerine renewable resourcess such as (can be got by glucose fermentation) be raw material, production cost is relatively low, and product selectivity is good, transformation efficiency is high, product separates simply, produces cleaning, advantages such as environmentally safe.Therefore, research and utilization microorganisms producing 1, the work of ammediol receives much attention.Intestinal bacteria metabolism glycerine synthetic 1, ammediol exists two metabolism routes: one is oxidative pathway, be provided for the energy and the carbon source of cell growth and generation NADH, this approach is that glycerine is under the effect of glycerol dehydrogenase, Protosol kinases etc., be converted into pyruvic acid, by anaerobism glycolysis approach, generate by products such as acetate, ethanol, lactic acid again.This path is the process that generates bioenergy ATP and reducing equivalent NADH.With K. pneumonia and lemon bacterium is example,
Glycerine+NAD
+→ DHA (Protosol)+NADH+H
+(1)
DHA+ATP→DHAP+ADP (2)
Another is the reduction approach, and glycerine is by glycerol dehydratase, 1, ammediol redox enzymic synthesis 1, ammediol.In this path, 1, ammediol is no longer fallen by further metabolism, therefore has a high density accumulation,
Glycerine → 3-HP (3-hydroxy propanal)+H
2O (3)
3-HP+NADH
+H+→1，3-PD+NAD
+ (4)
Endobacillary oxidative pathway and reduction approach are to pass through NADH/NAD
+And energy ATP/ADP coupling is associated in together.In oxidative pathway, produce NADH; In the reduction approach, NADH is as 1, the electron donor of ammediol oxydo-reductase.Oxidative pathway also provides energy for the conversion process of reduction approach, also is the metabolic part of thalli growth simultaneously.Utilize entero-bacte fermentative production 1, ammediol, the synthetic of the growth of cell and product is to be mutually related, the growth of microorganism cells runs through the whole process of fermentation all the time.
Problems such as but present fermentative Production 1, ammediol exist that production concentration is low, the production cycle is long and glycerol conversion yield is low cause lacking the market competitiveness and can't scale operation.Therefore improve the production intensity of fermentation and the key that transformation efficiency is industrial production cost.The zymotechnique that document is reported is when considering how to improve glycerol conversion yield, the general fermenting process anaerobism that adopts transforms, there is the lag phase of a growth in anaerobically fermenting, and thalli growth is slow, causes in the fermenting process biomass on the low side, influence metabolic reaction speed, cause the production cycle long, production intensity is low, and the final substrate that ferments is residual, therefore improve the biomass in the fermenting process, adopting high cell density fermentation is very important for improving production intensity, shortening the production cycle.
Summary of the invention
Technical problem: the object of the present invention is to provide a kind of biological fermentation that can improve to prepare 1, ammediol technological process production intensity, shorten the production cycle, the klebsiella high cell density fermentation that utilizes that reduces production costs produces 1, the method for ammediol.
Technical scheme: method of the present invention provides a kind of rapid batch fermentation method of multistep that microorganism cells can be grown with big specific growth rate in the batch fermentation phase, and involved entero-bacte can be klebsiella (Klebsiella), Fu Shi lemon bacterium (Citrobacter freundii), aerogenesis klebsiella (klebsiallaaerogenes), acid-producing Klebsiella bacterium (Klebsiella oxytoca).
The present invention adopts the high cell density fermentation technological process of batch fermentation mode to be:
1) seed culture process: after the klebsiella bacterial classification is activated, be seeded in the aseptic seed nutrient solution, 35 ℃～38 ℃, place shaking table to carry out aerobic seed culture, reach OD until cell density
650〉=4.0;
2) fermentation culture culturing process: fermentation culture is after the sterilization cooling, add defoamer, seed liquor is inoculated in the fermentor tank that fermentation culture is housed with the inoculum size of volume ratio 3～15%, middle every sampling in 2～4 hours, survey cell concentration, carbon source concentration and product 1, ammediol concentration;
3) batch fermentation thalline fast growing period: the concentration stabilize of carbon source is at 0.1～30g/L in the fermentation culture, and bubbling air in fermentation culture is controlled at DO5～25% with dissolved oxygen, keeps thalline to grow fast; 34～38 ℃ of controlled temperature, pH5～8,2～5 hours time;
4) high cell density fermentation period: when cell density reaches OD
6502.0～5.0; Take the restricted mode of adding with the restricted supply of air of nutraceutical matrix, make cell have only a spot of growth, synthesize the master with the high strength product;
When 5) having product to synthesize, infeeding in fermentor tank simultaneously can be by the nitrogenous source of thalli growth and trans-utilization, phosphoric acid salt, mineral ion, trace element, V
B12In one or more combination, flow glycerol adding simultaneously, make glycerol concentration remain on 20～40g/L.
6) no longer produce acid when fermenting process, fermenting process finishes, and produces 1, ammediol.
Possess the required nutritive ingredient of klebsiella growth in fermentation culture or the seed culture fluid, comprise carbon source, nitrogenous source, phosphoric acid salt, vitriol and other nutrition source; Wherein carbon source is starch, dextrin, glycerine, glucose, lactose, fructose, sucrose, isomaltose etc., concentration range 0.1～30g/L; Nitrogenous source is peptone, corn steep liquor, yeast extract paste, yeast powder, (NH
4)
2SO
4, NH
4Cl, halfcystine, Methionin, methionine(Met), glycine, concentration range 0.1～10g/L; Phosphoric acid salt is phosphate anion; Other nutrition source is inorganic salts potassium, sodium, magnesium, calcium metal-salt and trace element zinc, copper, boron, molybdenum, nickel, and micronutrient levels is at 0.01～50mg/L.
The restricted nutraceutical matrix of adding is nitrogenous source or phosphorus source, or the nitrogenous source source that phosphorates, and nitrogenous source is peptone, corn steep liquor, yeast extract paste, yeast powder, (NH
4)
2SO
4, NH
4Cl, halfcystine, Methionin, methionine(Met), glycine; The phosphorus source is a phosphate anion; Concentration range 0.1～10g/L.The air restriction is taked to reduce air flow, is gone up pressurize, air air flow 0～3.0V/Vmin or logical nitrogen 0.3～3.0V/Vmin.
The mode that nutraceutical matrix is added is that constant speed stream adds or variable flow adds or index stream adds or disposable adding; Also need to add glycerine to guarantee having enough transformation of glycerol to become 1 in this stage, ammediol, glycerol concentration remain on 20～40 g/L.
Beneficial effect: the invention provides a kind of rapid batch fermentation method of multistep that the klebsiella cell can be grown with big specific growth rate in the batch fermentation phase.Under given condition, guaranteed high production intensity, make whole bio-conversion process cycle short, substrate noresidue or low residue, can obtain again higher 1, ammediol concentration and high molar yield.This zymotechnique simple economy has improved the reactor production intensity, has shortened fermentation period, reduce production cost, helped the back extraction process again, be biosynthesizing 1, the industrialization of ammediol provides the zymotechnique of simple fast, economical, greatly improves the competitive power on market.
At utilizing entero-bacte ferment glycerin synthetic 1, in the technological line of ammediol, synthetic interrelated, the growth performance of producing bacterium of microbial growth and product is to the synthetic characteristic with remarkably influenced of product, take the rapid batch fermentation method of multistep, promptly earlier fermentation by ventilation oxygen-supplying in fermentor tank, microorganism growth desired nutritional matrix is provided, allow microorganism with big specific growth rate growth, reach high cell density at short notice; Later stage take the restricted stream of nutraceutical matrix to add and reduces the mode of oxygen supply in fermentation, make cell have only a spot of growth, synthesize the master with the high strength product.Use described method, both guaranteed high production intensity, make whole bio-conversion process cycle short, substrate noresidue or low residue, can obtain again higher 1, ammediol concentration and high molar yield.
High cell density fermentation all has very important significance to enhancing productivity, reduce production costs, simplify purifying products technology and reducing to enlarge to produce to invest.This technology is compared with domestic other production technique, and fermentation period is short, generally at 20～30 hours.No substrate or low substrate are residual, and fermentation ends residual glycerol content is lower than 0.5%.The production intensity height, fermentation ends 1, ammediol concentration reaches 40～65g/L, and specific production rate is greater than 1.5g/ (Lh), and molar yield reaches 50～70%; Adopt high density fermentation, improve fermentative production intensity, reduced production cost.
Embodiment
The present invention adopts the high cell density fermentation technological process of batch fermentation mode to be:
1) the seed culture process is: after the klebsiella bacterial classification is activated, be seeded in the aseptic seed nutrient solution, 35 ℃～38 ℃, place shaking table to carry out aerobic seed culture, reach OD until cell density
650〉=4.0;
2) fermentation culture is: fermentation culture is after the sterilization cooling, add defoamer, seed liquor is inoculated in the fermentor tank that fermentation culture is housed with the inoculum size of volume ratio 3～15%, middle every sampling in 2～4 hours, survey cell concentration, carbon source concentration and product 1, ammediol concentration;
3) batch fermentation thalline fast growing period: in the fermentation culture concentration stabilize of carbon source at 0.1～30g/L, best 1-3g/L, bubbling air in fermentation culture is controlled at DO5～25% with dissolved oxygen, keeps thalline to grow fast; 34～38 ℃ of controlled temperature, pH5～8,2～5 hours time;
6) high cell density fermentation period: when cell density reaches OD
6502.0～5.0; Take the restricted mode of adding with the restricted supply of air of nutraceutical matrix, make cell have only a spot of growth, synthesize the master with the high strength product;
When 7) having product to synthesize, infeeding in fermentor tank simultaneously can be by the nitrogenous source of thalli growth and trans-utilization, phosphoric acid salt, mineral ion, trace element, V
B12In one or more combination, flow glycerol adding simultaneously, make glycerol concentration remain on 20～40g/L.
6) no longer produce acid when fermenting process, fermenting process finishes, and produces 1, ammediol.
Fermentative preparation 1, ammediol, involved bacterium is klebsiella, Fu Shi lemon bacterium, aerogenesis klebsiella, acid-producing Klebsiella bacterium.
Possess the required nutritive ingredient of klebsiella growth in fermentation culture or the seed culture fluid, comprise carbon source, nitrogenous source, phosphoric acid salt, vitriol and other nutrition source; Wherein carbon source is starch, dextrin, glycerine, glucose, lactose, fructose, sucrose, isomaltose etc., concentration range 0.1～30g/L; Nitrogenous source is peptone, corn steep liquor, yeast extract paste, yeast powder, (NH
4)
2SO
4, NH
4Cl, halfcystine, Methionin, methionine(Met), glycine, concentration range 0.1～10g/L; Phosphoric acid salt is phosphate anion; Other nutrition source is inorganic salts potassium, sodium, magnesium, calcium metal-salt and trace element zinc, copper, boron, molybdenum, nickel, and micronutrient levels is at 0.01～50mg/L.
The restricted nutraceutical matrix of adding is nitrogenous source or phosphorus source, or the nitrogenous source source that phosphorates, and nitrogenous source is peptone, corn steep liquor, yeast extract paste, yeast powder, (NH
4)
2SO
4, NH
4Cl, halfcystine, Methionin, methionine(Met), glycine; The phosphorus source is a phosphate anion; Concentration range 0.1～10g/L, preferably 0.5-2 g/L.
The air restriction is taked to reduce air flow, is gone up pressurize, air air flow 0～3.0V/Vmin or logical nitrogen 0.3～3.0V/Vmin.
The mode that nutraceutical matrix is added is that constant speed stream adds or variable flow adds or index stream adds or disposable adding; Also needing to add glycerine in this stage has enough transformation of glycerol to become 1 with assurance, ammediol, and glycerol concentration remains on 20～40g/L.
Embodiment one:
Bacterial classification: Cray uncle pneumobacillus (Klebsiella pneumoniae) seed culture fluid (mass concentration, g/L): glycerine 10, glucose 10, K
2HPO
43H
2O, 3.4, NH
4Cl2.0, yeast extract paste 3.0, MgSO
47H
2O 0.2, CaCO
32.0, ferrous solution 1mL/L, trace element solution 2mL/L.
Fermentation culture (mass concentration, g/L): glycerine 2.0, K
2HPO
43H
2O 1.36, NH
4Cl 5.4, yeast extract paste 1.0, MgSO
47H
2O 0.2, CaCl
22.0 ferrous solution 1mL/L, trace element solution 2mL/L, V
B12Solution 1mL/L, cobalt liquor 1mL/L.Trace element contains: CuCl
2, H
3BO
3, MnCl
2, Na
2MoO
4, NiCl
2, ZnSO4, micronutrient levels is about 0.01～50mg/L.
The seed liquor culturing process: slant strains is seeded in the 500mL triangular flask that 150mL aseptic seed nutrient solution is housed after 24h activation, and 37 ℃, 180r/min carry out aerobic seed culture in shaking table, reach OD until cell density
650〉=4.0
Batch fermentation thalline fast growing period: 115 ℃ of sterilizations of fermentation culture 20min, after being cooled to 37 ℃, add defoamer, inoculum size with 3～15% is inoculated in seed liquor in the 5L fermentor tank that fermentation culture is housed, middle every 2～4 hours sampling 2mL, survey cell concentration, carbon source concentration and product 1, ammediol concentration.
Batch fermentation thalline fast growing period, stream adds carbon source 20% glucose 50mL, air air flow 0.3～3.0VVmin, and control dissolved oxygen DO value is at 5-15%, later stage stream adds 8% yeast extract paste solution, 50 mL, 4% potassium phosphate,monobasic solution 50mL keeps pH6.5-7.5 with 2mol/L NaOH and 2mol/L HCl, ferments, fermentation condition is as follows: 34～38 ℃ of temperature, mixing speed 300～500r/min, pH6.5～7.5, air flow 0～3.0V/Vmin or logical nitrogen 0.3～3.0V/Vmin.Also need to add glycerine in this stage and make glycerol concentration remain on 20～40g/L, to guarantee having enough transformation of glycerol to become 1, ammediol.
Experimental result:
Table 1 high cell density fermentation produces 1, the ammediol result
| Incubation time (hr) | 2hr30min | 4hr | 6hr30min | 9hr30min | 13hr | 27hr | |
| Air flow V/Vmin | 0.5 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | |
| Rotating speed r/min | 400 | 400 | 300 | 300 | 300 | 300 | |
| Cell density OD 650 | 2.0 | 4.6 | 6.8 | 7.2 | 7.6 | 7.4 | |
| Molar yield | 58.3％ | ||||||
| Specific production rate g/ (Lhr) | 1.74 | ||||||
| The finished product 1, ammediol concentration g/L | 47g/L | ||||||
27 hours this batch fermentation process cycles, final product 1, ammediol concentration is 47g/L.Batch fermentation aerating oxygen in early stage, biomass increases with fast speed, and institute's time-consuming is about 2～5 hours, cell density OD
650At (the obstructed oxygen of general technology, 12hr cell density OD more than 2.0
650About 0.4～1.2); Enter the later stage conversion process this moment, reduce the air air flow to 0.1VVm, stream adds 8% yeast extract paste 50mL, and 4% potassium phosphate,monobasic 50mL is to keep the glycerine metabolic balance.When the biological fermentation end of synthesis, cell density OD
650Be 7.4, fermentation period 27hr (under the same concentration conditions, anaerobism transforms generally needs 40～65 hr), glycerine is residual＜and 0.1%, final product 1, ammediol concentration is 47g/L.
Embodiment two:
Take identical seed and fermention medium, identical nutraceutical matrix stream adds technology, the batch fermentation later stage take respectively to reduce air flow, on modes such as pressurize, logical nitrogen, the gained result is:
Table 2 ventilation control is to fermentation result's influence
| Cell density OD 650 | Fermentation period | Glycerine is residual | 1, ammediol concentration | Molar yield | Specific production rate | |
| Reduce air flow | 7.4 | 27hr | ＜0.1％ | 47g/L | 58.3％ | 1.74g/(L·h) |
| Last pressurize | 6.8 | 31hr | ＜0.1％ | 48g/L | 59.6％ | 1.55g/(L·h) |
| Logical nitrogen | 6.8 | 29hr | ＜0.1％ | 51g/L | 63.3％ | 1.76g/(L·h) |
Can draw and take the restricted stream of nutrition to add mode with oxygen segmentation regulation and control, under the high-cell density condition, the batch fermentation later stage take respectively to reduce air flow, on mode bio-catalytic conversion of glycerol synthetic 1 such as pressurize (pressurize of ventilating on the fermented liquid liquid level), logical nitrogen, ammediol, all can obtain higher 1, ammediol throughput and high molar yield.
Can prove that by above-mentioned experiment the klebsiella high cell density fermentation that utilizes proposed by the invention produces 1, the method for ammediol, fermentation period is short, production intensity height, technology simple economy.This technology is compared with domestic other production technique, and fermentation period is short, generally at 20～30 hours.No substrate or low substrate are residual, and fermentation ends residual glycerol content is lower than 0.5%.The production intensity height, fermentation ends 1, ammediol concentration reaches 40～65g/L, and specific production rate is greater than 1.5g/ (Lh), and molar yield reaches 50～70%; Adopt high density fermentation, improve fermentative production intensity, reduced production cost, simplification purifying products technology and reduction expansion are produced investment and are all had very important significance.
Claims (5)
1, a kind of klebsiella high cell density fermentation that utilizes produces 1, and the method for ammediol is characterized in that adopting the high cell density fermentation technology of batch fermentation mode, and its technological process is
1) the seed culture process is: after the klebsiella bacterial classification is activated, be seeded in the aseptic seed nutrient solution, 35 ℃～38 ℃, place shaking table to carry out aerobic seed culture, reach OD until cell density
650〉=4.0;
2) fermentation culture is: fermentation culture is after the sterilization cooling, add defoamer, seed liquor is inoculated in the fermentor tank that fermentation culture is housed with the inoculum size of volume ratio 3～15%, middle every sampling in 2～4 hours, survey cell concentration, carbon source concentration and product 1, ammediol concentration;
3) batch fermentation thalline fast growing period: the concentration stabilize of carbon source is at 0.1～30g/L in the fermentation culture, and bubbling air in fermentation culture is controlled at DO5～25% with dissolved oxygen, keeps thalline to grow fast; 34～38 ℃ of controlled temperature, pH5～8,2～5 hours time;
4) high cell density fermentation period: when cell density reaches OD
6502.0～5.0; Take the restricted mode of adding with the restricted supply of air of nutraceutical matrix, make cell have only a spot of growth, synthesize the master with the high strength product;
When 5) having product to synthesize, infeeding in fermentor tank simultaneously can be by the nitrogenous source of thalli growth and trans-utilization, phosphoric acid salt, mineral ion, trace element, V
B12In one or more combination, flow glycerol adding simultaneously, make glycerol concentration remain on 20～40g/L.
6) no longer produce acid when fermenting process, fermenting process finishes, and produces 1, ammediol.
2, the klebsiella high cell density fermentation that utilizes according to claim 1 produces 1, the method of ammediol, it is characterized in that possessing in fermentation culture or the seed culture fluid the required nutritive ingredient of klebsiella growth, comprise carbon source, nitrogenous source, phosphoric acid salt, vitriol and other nutrition source; Wherein carbon source is one or more in starch, dextrin, glycerine, glucose, lactose, fructose, sucrose, the isomaltose, concentration range 0.1～30g/L; Nitrogenous source is peptone, corn steep liquor, yeast extract paste, yeast powder, (NH
4)
2SO
4, NH
4In Cl, halfcystine, Methionin, methionine(Met), the glycine one or more, concentration range 0.1～10g/L; Phosphoric acid salt is phosphate anion; Other nutrition source is one or more in inorganic salts potassium, sodium, magnesium, calcium metal-salt and trace element zinc, copper, boron, molybdenum, the nickel, and micronutrient levels is at 0.01～50mg/L.
3, the klebsiella high cell density fermentation that utilizes according to claim 1 produces 1, the method of ammediol, it is characterized in that the restricted nutraceutical matrix of adding is nitrogenous source or phosphorus source, or the nitrogenous source source that phosphorates, nitrogenous source is peptone, corn steep liquor, yeast extract paste, yeast powder, (NH
4)
2SO
4, NH
4Cl, halfcystine, Methionin, methionine(Met), glycine; The phosphorus source is a phosphate anion; Concentration range 0.1～10g/L.
4, the klebsiella high cell density fermentation that utilizes according to claim 1 produces 1, and the method for ammediol is characterized in that the air restriction takes to reduce air flow, goes up pressurize, air air flow 0～3.0V/Vmin or logical nitrogen 0.3～3.0V/Vmin.
5, the klebsiella high cell density fermentation that utilizes according to claim 3 produces 1, and the method for ammediol is characterized in that the mode that nutraceutical matrix is added is that constant speed stream adds or variable flow adds or index stream adds or disposable adding; Also needing to add glycerine in this stage has enough transformation of glycerol to become 1 with assurance, ammediol, and glycerol concentration remains on 20～40g/L.
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| CN100463968C (en) * | 2006-01-27 | 2009-02-25 | 华侨大学 | Process for preparing 1,3-propylene glycol and dihydroxy acetone by bio-catalytic conversion of glycerol |
| WO2007095255A2 (en) * | 2006-02-10 | 2007-08-23 | Dupont Tate & Lyle Bio Products Company, Llc | Biodegradable compositions comprising renewably-based, biodegradable 1.3-propanediol |
| US20070207939A1 (en) * | 2006-02-10 | 2007-09-06 | Gyorgyi Fenyvesi | Compositions comprising mono and di esters of biologically-based 1,3-propanediol |
| CN101323863B (en) * | 2008-07-18 | 2012-01-04 | 东南大学 | Method for improving concentration of 1,3-propanediol produced by microbial fermentation |
| US9968531B2 (en) | 2015-08-05 | 2018-05-15 | Dupont Tate & Lyle Bio Products Company, Llc | Deodorants containing 1,3-propanediol |
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| CN1348007A (en) * | 2001-04-27 | 2002-05-08 | 大连理工大学 | Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol |
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