CN1327798A

CN1327798A - Process for preparing low-peptide mixer by controlled enzymolysis of snake

Info

Publication number: CN1327798A
Application number: CN01114565A
Authority: CN
Inventors: 卢南洲
Original assignee: YISHE BIOLOGIC SCIENCE AND Technology CO LTD YONGZHOU
Current assignee: YISHE BIOLOGIC SCIENCE AND Technology CO LTD YONGZHOU
Priority date: 2001-06-28
Filing date: 2001-06-28
Publication date: 2001-12-26

Abstract

A process for preparing a low-peptide mixture by controllable enzymolysis of snake includes such steps as killing snake, mincing, grinding, regulating concentration, adding compound enzyme, constant-temp hydrolysis, and deactivating enzyme. Said mixture is rich in nutrients and can be easily absorbed, so it can be used to make food, health-care product, or medicine. Its advantages are less loss of active components and high absorption rate.

Description

A kind of Serpentis becomes the process of low-peptide mixer through the control enzymolysis is arranged

The present invention relates to and a kind ofly make catalyst, controlledly Serpentis animal proteinum substrate is carried out the process of biological enzymolysis with compound protease.

Final products to Serpentis animal proteinum (Carnis Serpentis) processing are generally snake powder and oral liquid.Conventional machining process or be with Carnis Serpentis oven dry after pulverize and make snake powder, or will make oral liquid with modulation after filtration after its steaming and decocting.The shortcoming of producing Serpentis animal proteinum product by above-mentioned process has two, one, and Carnis Serpentis is after the long period high-temperature process, and its biological activity has greater loss; The 2nd, the protein form that Carnis Serpentis mainly is still with macromolecule in product exists, and it is subjected to certain restriction intravital the digesting and assimilating of people.Traditional research thinks that protein mainly is to be absorbed with amino acid whose form through after the effect of digestive enzyme, and the past also has the people that the Serpentis animal proteinum is made aminoacid mixing liquid after complete hydrolysis, to improve the absorbance of human body to the Serpentis animal proteinum.And discovering recently, protein is through behind the digestive tract, mainly is that the form with low peptide absorbs, and the absorbance that people have confirmed low peptide by the animal metabolism test is than aminoacid height, and low peptide is absorbed by body by mucous membrane of small intestine than aminoacid is easier, faster.According to above-mentioned achievement in research, can be prepared into low-peptide mixer with behind the biological enzymolysis of Serpentis animal proteinum through control is arranged, make product through relevant subsequent technology again, the crowd that can not fully satisfy the protein needs for those common diet replenishes protein.

The object of the present invention is to provide a kind of Serpentis to become the process of low-peptide mixer to produce the white low-peptide mixer of snake egg through the control enzymolysis is arranged.This technical process reaction condition gentleness, its biological activity loss is little, and it is low-peptide mixer that reaction formation owner wants composition, easier being absorbed by the body.

Technological process of the present invention is as follows:

Compound enzyme → constant temperature hydrolysis → enzyme denaturing → low-peptide mixer is modulated → added to apolegamy Serpentis alive → slaughter → rub → defibrination → Carnis Serpentis concentration.

1, apolegamy Serpentis alive:, select two or more different types of Serpentis hybrid process according to a certain ratio according to local resources situation and product design function.Though different types of Serpentis composition is variant slightly, through test determination, the technological parameter of its biological enzymolysis generation low-peptide mixer is basic identical.

2, slaughter: decaptitate after Serpentis kills, internal organs and skin, clean the back and dry.

3, rub: put the Carnis Serpentis (band bone) that dries into meat grinder and rub, meat grinder discharging opening granule full-size aspect should be no more than 2mm, does not accomplish to carry out multistage rubbing if one-level is rubbed.

4, defibrination: putting into colloid mill through the mach Carnis Serpentis of Minced Steak, it is worn into slurry, the Carnis Serpentis particle diameter of wearing into slurry must reach micron order.

5, Carnis Serpentis concentration modulation: Carnis Serpentis is starched the retort of putting into blender, add 1～1.5 times of clean water, stir, make concentration of substrate (solid content) reach 10% to Carnis Serpentis slurry weight.

6, add compound enzyme: should add protease weight by substrate weight (releasing rare weight) calculating.During calculating, it is that 960 ∪/g calculates by enzyme one concentration of substrate ratio that papain is produced in Nanning.It is that 960 ∪/g calculates by enzyme one concentration of substrate ratio that neutral protease is produced in the Wuxi.Papain and neutral protease are added in the retort after compound simultaneously.

7, constant temperature hydrolysis: constant temperature after the retort temperature risen to 50 ℃, allow the agitator continuous running, only allow its hydrolysis 4 hours, the time can not be long, otherwise partly low-peptide mixer can transform to free amino acid.

8, enzyme denaturing: the retort temperature is risen to 80 ℃ rapidly, and constant temperature is 5 minutes then, makes protease force passivation, loses vigor, and rapidly the retort temperature is reduced to room temperature then.So far, technical process of the present invention finishes, and the material that obtains in the retort is the low-peptide mixer of Serpentis animal proteinum and the solidity thing of partly not finishing hydrolysis, at this moment, the Carnis Serpentis percent hydrolysis can reach more than 60%, and the hydrolysis composition low-peptide mixer of controlling with this technological parameter accounts for more than 80%.

If target product is a snake powder, just the direct spray drying of material in the above-mentioned retort can be obtained snake powder.If target product is an oral liquid, the back fill can be modulated with batching after filtration by thing section in the above-mentioned retort.If target product can carry out according to other subsequent technique for some other products.

Embodiment one: get Agkistrodon 2kg alive, Zaocys 8kg decaptitates after killing, internal organs, skin, cleans the back and dry with drier.The Carnis Serpentis that dries is put into pulverizer rub, put into colloid mill again it is worn into the meat slurry.Meat is starched the retort of putting into agitator, add the 15kg deionized water and stirring, and constant temperature after making temperature rise to 50 ℃ rapidly.The neutral protease of 2,400 ten thousand units and the papain of 2,400 ten thousand units are mixed in the back adding retort, and the constant temperature enzymolysis made temperature rise to 5 minutes enzyme denaturing of 80 ℃ of constant temperature rapidly after 4 hours, reduced to room temperature then rapidly, obtained the low-peptide mixer hydrolyzed solution.With the hydrolyzed solution spray drying, the powder that obtains is the low-peptide mixer pure snake powder of Agkistrodon and Zaocys proportioning.Carry out capsule at last and fill, the dress box was put in storage after bubble was moulded packing making sheet and microwave sterilizating.

Embodiment two: with the low-peptide mixer hydrolyzed solution that obtains among the embodiment 1 through tubular type centrifugal filtration, put it in the mixer of agitator, stir after adding suitably flavored agent and Chinese medicine extract, allow its fill behind flash pasteurization become every bottle of 10ml Serpentis nutritional solution again.After microwave sterilizating, lamp inspection, the back dress box warehouse-in of labelling.

Claims

1, a kind of Serpentis becomes the process of low-peptide mixer through the control enzymolysis is arranged, and it comprises following flow process: select materials, slaughter, rubbing, defibrination, concentration modulation, add compound enzyme, constant temperature hydrolysis, enzyme denaturing; Select by a certain percentage two or more different types of Serpentiss alive through slaughter, decaptitate, internal organs and skin, clean, drying, rubbing, defibrination carry out the modulation of Carnis Serpentis slurry concentration after becoming particle diameter to be micron-sized Carnis Serpentis slurry, add compound enzyme, constant temperature hydrolysis and enzyme denaturing operation is characterized in that:

1. in having the retort of blender, carry out the modulation of Carnis Serpentis concentration, must doubly stir, make concentration of substrate (solid content) reach 10% toward adding 1-1.5 in the jar to the clean water of Carnis Serpentis slurry weight;

2. add compound enzyme kind and consumption, calculate the weight that adds papain and neutral protease, its preparation ratio=papain units: substrate weight=neutral protease units: substrate weight=960 units/gram by the substrate weight of releasing after rare;

3. constant temperature hydrolysis: temperature is 50 ℃, stirs 4 hours;

4. enzyme denaturing, 80 ℃ of temperature, constant temperature 5 minutes, speed is cooled to room temperature.