CN1326576C - Biological restoration material, prepn. method and applicatino thereof - Google Patents
Biological restoration material, prepn. method and applicatino thereof Download PDFInfo
- Publication number
- CN1326576C CN1326576C CNB2005100987315A CN200510098731A CN1326576C CN 1326576 C CN1326576 C CN 1326576C CN B2005100987315 A CNB2005100987315 A CN B2005100987315A CN 200510098731 A CN200510098731 A CN 200510098731A CN 1326576 C CN1326576 C CN 1326576C
- Authority
- CN
- China
- Prior art keywords
- tris
- buffer
- handled
- hcl
- fascia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000463 material Substances 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title description 6
- 210000003195 fascia Anatomy 0.000 claims abstract description 25
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims abstract description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 15
- 102000004142 Trypsin Human genes 0.000 claims abstract description 9
- 108090000631 Trypsin Proteins 0.000 claims abstract description 9
- 239000011780 sodium chloride Substances 0.000 claims abstract description 9
- STCOOQWBFONSKY-UHFFFAOYSA-N tributyl phosphate Chemical compound CCCCOP(=O)(OCCCC)OCCCC STCOOQWBFONSKY-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012588 trypsin Substances 0.000 claims abstract description 9
- 210000002569 neuron Anatomy 0.000 claims abstract description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 230000001464 adherent effect Effects 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 4
- 230000003387 muscular Effects 0.000 claims description 3
- 230000008439 repair process Effects 0.000 claims description 3
- 239000000835 fiber Substances 0.000 abstract description 11
- 208000020431 spinal cord injury Diseases 0.000 abstract description 11
- 210000004027 cell Anatomy 0.000 abstract description 10
- 230000002163 immunogen Effects 0.000 abstract description 2
- 238000003672 processing method Methods 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- 239000007853 buffer solution Substances 0.000 abstract 5
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 19
- 229920001436 collagen Polymers 0.000 description 15
- 102000008186 Collagen Human genes 0.000 description 6
- 108010035532 Collagen Proteins 0.000 description 6
- 210000003205 muscle Anatomy 0.000 description 5
- 230000001537 neural effect Effects 0.000 description 5
- 210000003618 cortical neuron Anatomy 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 210000000278 spinal cord Anatomy 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 210000005036 nerve Anatomy 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 2
- 210000003050 axon Anatomy 0.000 description 2
- 230000028600 axonogenesis Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 239000000512 collagen gel Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 229920001606 poly(lactic acid-co-glycolic acid) Polymers 0.000 description 2
- 229920002187 poly[N-2-(hydroxypropyl) methacrylamide] polymer Polymers 0.000 description 2
- 229920002338 polyhydroxyethylmethacrylate Polymers 0.000 description 2
- 230000035807 sensation Effects 0.000 description 2
- 210000001032 spinal nerve Anatomy 0.000 description 2
- 210000002435 tendon Anatomy 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000001738 Nervous System Trauma Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000589 cicatrix Anatomy 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- PIDANAQULIKBQS-RNUIGHNZSA-N meprednisone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@@H]2[C@@H]1[C@@H]1C[C@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)CC2=O PIDANAQULIKBQS-RNUIGHNZSA-N 0.000 description 1
- 229960001810 meprednisone Drugs 0.000 description 1
- -1 methyl meticortelone Chemical compound 0.000 description 1
- 208000028412 nervous system injury Diseases 0.000 description 1
- 230000000324 neuroprotective effect Effects 0.000 description 1
- 230000008058 pain sensation Effects 0.000 description 1
- 210000000578 peripheral nerve Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 210000005070 sphincter Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Materials For Medical Uses (AREA)
- Prostheses (AREA)
Abstract
The present invention discloses a biological restoration material, and a preparation method and the application thereof. The biological restoration material of the present invention is obtained according to the following steps: 1) a fascia is processed for 24 to 72 hours by a tributyl phosphate solution of which the volume percentage concentration is from 1 to 1.5 %; 2) then, the fascia is processed for 24 to 72 hours by a Tris-HCl buffer solution of 25 to 100 mmol/L, the Tris-HCl buffer solution contains NaCl of 0.5 to 1.5 mol/L, and the pH of the Tris-HCl buffer solution is from 7.6 to 8.5; 3) a buffer solution with trypsin of 0.5 to 1.5g/100 ml is used for processing for 48 to 96 hours, the buffer solution is the Tris-HCl of 25 to 100 mmol/L, and the pH is from 7 to 8 so as to obtain the biological restoration material; processing temperature is from 2 to 8 DEG C. The biological restoration material of the present invention uses a natural fascia as a foundation, and is obtained through a series of unique processing methods. The present invention has the advantages of favorable affinity for cells, and low immunogen. The present invention can orient neurons, so that the neurons grow in a fiber direction orderly, which is important for the recovery of the injury of spinal cords in a body.
Description
Technical field
The present invention relates to bioprosthetic material and preparation method thereof and application.
Background technology
Spinal cord is the bridge that connects periphery and central nervous system, and it conducts to maincenter (brain) with sensation (as the pain sensation, thermo aesthesia, the sense of touch) stimulation of health, and also the instruction with brain is transmitted to the motion muscle group and produces random motion.In addition, also be responsible for some neural reflexs.Because the transverse injury of spinal cord that various factors (wound, inflammation, tumor etc.) has caused is called as spinal cord injury (SCI), this disease has caused the obstacle of the following function of spinal nerves (motion, sensation, sphincter and vegetative nerve function) in infringement plane.
How to promote neuranagenesis and functional rehabilitation behind the SCI, be the medical circle a great problem always.A very long time, it is believed that impaired nervus centralis is can't be regenerated.Up to 1981, the neuroscientist confirmed in basic research that just the nerve reparation behind the central nervous system injury is possible, yet effectively treatment means still lacks.It is very little that conventional operation means have been proved effect, can only make the thorough rehabilitation of only a few people among these the wounded.The most effective medicine of generally acknowledging has only methyl meticortelone (methylprednisone) at present, it is a kind of anti-inflammation drugs, confirmed the spinal cord condition in damaged is alleviated by a large amount of clinical experiments, but it must be after spinal cord injury uses immediately, for 24 hours later spinal cord patients poor effect then.The situation of this treatment means scarcity does not all obtain changing for a long time.
Rise along with the organizational project subject, many methods are attempted being used for treating spinal cord injury, as the implantation method reparation, comprise that peripheral nerve, embryo's nervus centralis, various kinds of cell all are proved the microenvironment that can improve the spinal cord injury position, promote the regeneration and the reparation of Spinal Cord.Some trophic factors and medicine also are proved as cAMP and NGF etc. and can promote growing and extend of aixs cylinder.But well-known, very short time after the spinal cord injury, formed cavity and cicatrix at damaged part, under such hostile environment, aixs cylinder promptly allows to growth, but also is difficult to go beyond a such obstacle.So people attempt using timbering material, when promoting axon elongation, provide support to axon growth, as collagen gel and a kind of by poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) be the NeuroGel[2 of raw material, 3], they provide good support can both for neuronic growth, do not solve but exist a common issue with, i.e. the aixs cylinder of directed these extensions how.Because in human body, the growth of spinal nerves fiber (aixs cylinder) is directive, and this just orientation just makes and transmits Nerve impulse accurately and effectively.So when utilizing various medicines and graft, the directed neure growth of guide measure that needs are special, otherwise aixs cylinder is understood non-directional growth, and can not play the effect of accurate transmission signal.
Under such demand, neural guiding biomaterial arises at the historic moment.Generally speaking, ideal neural guide material need possess following characteristics:
(1) orderly structure is with the neuronic growth of orientation, and enough cell growing spaces are provided;
(2) excellent biological compatibility can the sustenticular cell normal growth, and does not have immunogenicity;
(3) piped structure has formed the space of a relative closure, and the growth of neuroprotective unit is not subjected to the interference of other cell;
(4) has biodegradability.
At present, neural guide material is mainly made by the material of chemosynthesis, as poly (lactic-co-glycolicacid)] (PLGA) and poly (2-hydroxyethylmethacrylate) (PHEMA) etc., but that they exist cell compatibility is bad and easily cause the problem of immunologic rejection.The collagen scaffold that is used for repair of spinal cord injury now, mainly from the molten collagen preparation of acid, complex process, step is more, and main based on unordered tubulose and collagen gel.
Fascia (aponeurosis) is the protective tissue that is present in muscle surface; the extension that belongs to tendon; its main component is the same with tendon; constitute by I type and III type collagen fiber; its macrograph such as Figure 1A, Figure 1B (Figure 1A is the internal layer figure that fascia links to each other with muscle, and Figure 1B is the skin figure that fascia links to each other with muscle).But natural fascia structure is tight, and is not suitable for the attaching and the growth of cell.
Summary of the invention
The purpose of this invention is to provide a kind of structurally ordered bioprosthetic material and preparation method thereof.
Bioprosthetic material provided by the present invention, handle obtaining as follows:
1) with the fascia concentration expressed in percentage by volume is tributyl phosphate solution-treated 24-72 hour of 1-1.5%;
2) be that the Tris-HCl buffer of 7.6-8.5 was handled 24-72 hour with the 25-100mmol/L, the pH that contain 0.5-1.5mol/L NaCl then;
3) then, handled 48-96 hour with 0.5-1.5g trypsin/100ml buffer, described buffer is 25-100mmol/L Tris-HCl, and pH is 7-8; Obtain described bioprosthetic material;
The temperature of above-mentioned processing is 2-8 ℃.
In order further to improve the cell compatibility of material, the bioprosthetic material that obtains was also handled 5-10 minute for the 0.5-1.5mol/L strong base solution through over-richness.Highly basic commonly used has NaOH, KOH etc.
The solvent of the described tributyl phosphate solution of step 1) can be selected for use usually and be PBS buffer or Tris-HCl buffer.
The preferred for preparation scheme of bioprosthetic material of the present invention comprises the steps:
1) be 1% tributyl phosphate solution-treated 48 hours with the fascia concentration expressed in percentage by volume, solvent is 50mmol/L Tris-HCl, and pH is 8;
2) be that 8.0 Tris-HCl buffer was handled 48 hours with the 50mmol/L, the pH that contain 1mol/L NaCl then;
3) then, handled 72 hours with 1g trypsin/100ml buffer, described buffer is 50mmol/LTris-HCl, and pH is 8;
4) handled 5 minutes with 1mol/L NaOH, obtain described bioprosthetic material;
The temperature of above-mentioned processing is 4 ℃.
In order to guarantee the quality of material, fascia also will be removed adherent muscular tissue of internal layer and outer adherent fat before processing.
Another object of the present invention provides the application of bioprosthetic material of the present invention.
The inventor confirms that by experiment when growth in vitro, neuron can well be grown on bioprosthetic material of the present invention, aixs cylinder is along the fiber ordering growth, and can begin to interconnect, and can be used as the material of external repair of neuron.
The invention based on natural fascia, obtain a kind of novel bioprosthetic material through the processing method of a series of uniquenesses with orderly surface texture, this material pair cell has good affinity, low immunogen; And it can directed neuron along the direction ordering growth of fiber, this recovery for spinal cord injury in the body is crucial; In addition, this material can be easy to make lamellar, orderly tubular bracket or fiber according to different needs.Wherein, the tubular bracket that is made of ordered fiber has good application prospects, after being packed into collagen fiber in the pipe, not only can effectively increase the neure growth area, directed neuronic extension, and formed the space of neure growth relative closure, prevent the invasion of non-neural class cell.
Description of drawings
Fig. 1 is the macrograph of fascia;
Fig. 2 is the photo of bioprosthetic material of the present invention;
Fig. 3 is the growth photo of cortical neuron on natural fascia and bioprosthetic material of the present invention.
The specific embodiment
The preparation of embodiment 1, bioprosthetic material
One, draws materials and handle
1, pretreatment fascia
Get the fresh adult cattle muscle that has white fascia, with cold deionized water rinsing 3 times; Isolate fascia with tweezers and scalpel, remove tissues such as adherent muscular tissue of internal layer and outer field fat as far as possible.
2, material preparation
The pretreatment fascia is handled as follows:
1) in 1%TnBP (tributyl phosphate) solution (50mM Tris-HCl, pH=8.0, percent by volume) 48 hour;
2) handled 48 hours among the 50mM Tris-C1 buffer (pH=8.0 contains 1M NaCl);
3) 1g trypsin/100ml (50mM Tris-HCl buffer, pH=8.0) middle processing 72 hours;
4) handled 5 minutes among the 1M NaOH, fully clean until neutrality;
Lyophilizing promptly obtains bioprosthetic material of the present invention, called after apo-collagen.
Two, the form of material
Gained apo-collagen is the white plates form, can also be prepared as orderly tubulose or collagen fiber.The aspect graph of apo-collagen as shown in Figure 2, Fig. 2 a, Fig. 2 b and Fig. 2 c represent flaky, piped and fibrous apo-collagen, scale length=1cm respectively; Fig. 2 d is the stereoscan photograph of apo-collagen.Shown that by Fig. 2 apo-collagen has kept the structure of natural collagen fibre, its electromicroscopic photograph has shown that it has orderly surface texture, and its coarse surface texture also is beneficial to the attaching of cell.
The preparation of embodiment 2, bioprosthetic material
According to the method pretreatment fascia of embodiment 1, the pretreatment fascia is handled as follows:
1) in the 1.5%TnBP solution (50mM Tris-HCl, pH=8.0, percent by volume) 24 hour;
2) handled 72 hours among the 80mM Tris-C1 buffer (pH=7.8 contains 0.8M NaCl);
3) 0.5g trypsin/100ml (80mM Tris-HCl buffer, pH=7.8) middle processing 96 hours;
After the lyophilizing, promptly obtain bioprosthetic material of the present invention.
Properties of materials is identical with embodiment 1 gained material.
Embodiment 3,
According to the method pretreatment fascia of embodiment 1, the pretreatment fascia is handled as follows:
1) in the 1%TnBP solution (50mM Tris-HCl, pH=8.0, percent by volume) 48 hour;
2) handled 24 hours among the 100mM Tris-C1 buffer (pH=8.0 contains 1.3M NaCl);
3) 1.4g trypsin/100ml (30mM Tris-HCl buffer, handled 48 hours in pH=7.0):
4) handled 5 minutes among the 0.5M KOH, fully clean until neutrality;
Lyophilizing promptly obtains bioprosthetic material of the present invention.
Properties of materials is identical with embodiment 1 gained material.
The growth on apo-collagne of embodiment 4, neuron
The cortical neuron that separates rat is seeded on collagem membrane and the embodiment 1 gained material on (apo-collagen) 37 ℃ and 5%CO then respectively
2Cultivate under the condition, with the diacetate fluorescent staining, observe cultivation results after 5 days, as shown in Figure 3: Fig. 3 a is the growth of cortical neuron on collagem membrane; Fig. 3 b, Fig. 3 c and Fig. 3 d are the growth of cortical neuron on apo-collagen.
Shown in Fig. 3 a, neuron axon growth on collagem membrane is less, and the axon elongation direction very at random; And on apo-collagen, neure growth is good, and aixs cylinder is along fiber ordering growth (Fig. 3 b), and neuronic aixs cylinder begins to interconnect (Fig. 3 c, Fig. 3 d).
Claims (7)
1, a kind of bioprosthetic material, handle obtaining as follows:
1) with the fascia concentration expressed in percentage by volume is tributyl phosphate solution-treated 24-72 hour of 1-1.5%;
2) be that the Tris-HCl buffer of 7.6-8.5 was handled 24-72 hour with the 25-100mmol/L, the pH that contain 0.5-1.5mol/L NaCl then;
3) then, handled 48-96 hour with 0.5-1.5g trypsin/100ml buffer, described buffer is 25-100mmol/L Tris-HCl, and pH is 7-8; Obtain described bioprosthetic material;
The temperature of above-mentioned processing is 2-8 ℃.
2, the preparation method of the described bioprosthetic material of claim 1 comprises the steps:
1) with the fascia concentration expressed in percentage by volume is tributyl phosphate solution-treated 24-72 hour of 1-1.5%;
2) be that the Tris-HCl buffer of 7.6-8.5 was handled 24-72 hour with the 25-100mmol/L, the pH that contain 0.5-1.5mol/L NaCl then;
3) then, handled 48-96 hour with 0.5-1.5g trypsin/100ml buffer, described buffer is 25-100mmol/L Tris-HCl, and pH is 7-8; Obtain described bioprosthetic material;
The temperature of above-mentioned processing is 2-8 ℃.
3, preparation method according to claim 2 is characterized in that: the described bioprosthetic material that obtains was also handled 5-10 minute for the 0.5-1.5mol/L strong base solution through over-richness.
4, preparation method according to claim 2 is characterized in that: the solvent of the described tributyl phosphate solution of step 1) is PBS buffer or Tris-HCl buffer.
5, preparation method according to claim 2 is characterized in that: described preparation process comprises:
1) be 1% tributyl phosphate solution-treated 48 hours with the fascia concentration expressed in percentage by volume, solvent is 50mmol/LTris-HCl, and pH is 8;
2) be that 8.0 Tris-HCl buffer was handled 48 hours with the 50mmol/L, the pH that contain 1mol/L NaCl then;
3) then, handled 72 hours with 1g trypsin/100ml buffer, described buffer is 50mmol/LTris-HCl, and pH is 8;
4) handled 5 minutes with lmol/L NaOH, obtain described bioprosthetic material;
The temperature of above-mentioned processing is 4 ℃.
6, according to the arbitrary described preparation method of claim 2-5, it is characterized in that: described fascia also will be removed adherent muscular tissue of internal layer and outer adherent fat before processing.
7, the described bioprosthetic material of claim 1 is in the application of external repair of neuron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100987315A CN1326576C (en) | 2005-09-07 | 2005-09-07 | Biological restoration material, prepn. method and applicatino thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100987315A CN1326576C (en) | 2005-09-07 | 2005-09-07 | Biological restoration material, prepn. method and applicatino thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1751747A CN1751747A (en) | 2006-03-29 |
| CN1326576C true CN1326576C (en) | 2007-07-18 |
Family
ID=36678825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100987315A Expired - Fee Related CN1326576C (en) | 2005-09-07 | 2005-09-07 | Biological restoration material, prepn. method and applicatino thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1326576C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101979106B (en) * | 2010-09-10 | 2013-07-31 | 中国科学院遗传与发育生物学研究所 | Biological material for rehabilitating spinal cord injury and preparation method and application thereof |
| CN106256381A (en) * | 2015-06-18 | 2016-12-28 | 北京朗嘉仪生物技术有限公司 | Repair materials and its production and use in order |
| CN105497979B (en) * | 2015-12-24 | 2017-10-03 | 中国科学院遗传与发育生物学研究所 | Controllable functional material of guiding nerve regneration of degradation rate and preparation method and application |
| CN110787321A (en) * | 2018-08-01 | 2020-02-14 | 中国科学院遗传与发育生物学研究所 | Application of functional collagen scaffold LOCS + CBD-NT3 in repairing spinal cord injury |
| CN111110924B (en) * | 2019-12-18 | 2022-02-11 | 中国科学院遗传与发育生物学研究所 | Tissue engineering material for repairing nerve injury and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1228338A (en) * | 1999-02-04 | 1999-09-15 | 马昭若 | Wound covering material capable of promoting wound healing |
| CN1546182A (en) * | 2003-12-15 | 2004-11-17 | 中国人民解放军第四军医大学 | Tissue engineering compound material for repairing nerve injury and its preparation |
-
2005
- 2005-09-07 CN CNB2005100987315A patent/CN1326576C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1228338A (en) * | 1999-02-04 | 1999-09-15 | 马昭若 | Wound covering material capable of promoting wound healing |
| CN1546182A (en) * | 2003-12-15 | 2004-11-17 | 中国人民解放军第四军医大学 | Tissue engineering compound material for repairing nerve injury and its preparation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1751747A (en) | 2006-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1326576C (en) | Biological restoration material, prepn. method and applicatino thereof | |
| CN101979106B (en) | Biological material for rehabilitating spinal cord injury and preparation method and application thereof | |
| Komuro | Three-dimensional observation of the fibroblast-like cells associated with the rat myenteric plexus, with special reference to the interstitial cells of Cajal | |
| DE4204012A1 (en) | MITOGEN-FREE SUBSTANCE, THEIR PRODUCTION AND USE | |
| CN102813961A (en) | Injection gel containing submicron hyaluronic acid microspheres and preparation method thereof | |
| ITPD980149A1 (en) | THREE-DIMENSIONAL PROSTHESES INCLUDING HYALURONIC ACID DERIVATIVES TO REPAIR OR REBUILD DAMAGED TISSUES AND PROCESS FOR THE | |
| CN106668950A (en) | Fibroin three-dimensional bracket for nervus centralis remediation | |
| DE19906096A1 (en) | Protein with a heparin-binding epitope | |
| Baur et al. | Wound contractions, scar contractures and myofibroblasts: a classical case study | |
| CN109689120A (en) | Combination therapy for neurotrosis | |
| CN107412861A (en) | The Bone Defect Repari gel of recombined collagen sulfate composite chondroitin and polyethylene glycol | |
| KR20130109850A (en) | Kit comprising recombinant human bone morphogenetic protein for skin repair as active ingredient | |
| CN105457103A (en) | 3D-printed peripheral nerve conduit and preparing method thereof | |
| CN109106981A (en) | The collagen scaffold of dual modification and the application in the product for repairing spinal cord injury | |
| Shoffstall et al. | Engineering therapies in the CNS: What works and what can be translated | |
| CN111110832A (en) | A preparation containing snail extractive solution and Chinese Holly extract for treating wound and scar, and its preparation method | |
| CN113520899B (en) | Recombinant collagen product for skin photodamage repair | |
| BRPI0411236A (en) | method for producing retinal nerve cells from iris tissue-derived neural / progenitor stem cells and retinal nerve cells obtained from the same method | |
| CN1837239B (en) | Polypeptide growth factor copolymer and its preparation method and use | |
| CN106420399A (en) | Laser cosmetology nursing agent, preparation and use method thereof | |
| CN106075531A (en) | A kind of medical film of reducing swelling and alleviating pain | |
| Li et al. | Exploring the sensory function reconstruction by the combined surgery | |
| CN100562306C (en) | Contain the biological beauty skin care item of body keratinized cell growth factor-2 and the preparation method of recombinant human keratinized cell growth factor-2 | |
| CN1306824A (en) | Water soluble medical chitose preparation and its preparation | |
| Sun et al. | Electroacupuncture-enhanced differentiation of bone marrow stromal cells into neuronal cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |