CN1322483A - Licorice dreg fodder technology - Google Patents
Licorice dreg fodder technology Download PDFInfo
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- CN1322483A CN1322483A CN01117626A CN01117626A CN1322483A CN 1322483 A CN1322483 A CN 1322483A CN 01117626 A CN01117626 A CN 01117626A CN 01117626 A CN01117626 A CN 01117626A CN 1322483 A CN1322483 A CN 1322483A
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- glycyrrhiza residue
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
Licorice dreg fodder is produced by using licorice dreg as main material and the mixture of wheat bran, corn powder, cotton seed cake, NaCl, fish meal, red sugar, urea and lactic acid as supplementary material. Through biological process, the supplementary material is fermented with bacteria, ray fungi, saccharomycetes and filamentous fungi to obtain mixed spawn, and licorice dreg is then fermented with the mixed spawn to obtain licorice dreg fodder. The licorice dreg fodder is used in raising sheep and ox. The present inventino changes waste licorice dreg into useful fodder.
Description
The present invention relates to a kind of licorice dreg fodder technology
The Radix Glycyrrhizae resource of China is very abundant, extensive use and pharmacy, food, brewages and industry such as light industry, is the raw material of producing goods such as extract of licorice root or liquid extract, glycyrrhizin, enoxolone.A large amount of glycyrrhiza residue of Chan Shenging is generally only made the fertilizer sanitary fill in process of production, and simple extensive mode such as burning is handled, and has both wasted Limited resources, again environment is caused to a certain degree pollution.
At present, sheep husbandry, cattle-raising etc. are fattened generally based on roughages such as the cotton seed hulls of feeding, straw, storage grass in the animal husbandry.Though the content of cellulose of hay slag is higher, glycyrrhiza residue not only carbon source is abundant, but also contains many drug ingedients, has health care.Utilize the hay slag to prepare sheep husbandry, cattle-raising etc. and fatten roughage, can solve the effective way of energy feed resource shortage, also can alleviate pollution, can produce economic benefit again simultaneously, have great importance environment by biofermentation technique.
The object of the invention is, the licorice dreg fodder of development is to be raw material with the glycyrrhiza residue, with wheat bran, corn flour, cottonseed cake, sodium chloride, fish meal, brown sugar, to add the urea, the lactic acid that account for the raw material total amount in addition be that auxiliary material mixes, and makes glycyrrhiza residue roughage with the bacteria fermentation of preparation or with commercially available commodity starfish medicament fermentation or with commercially available commodity FP4 medicament fermentation respectively.Bacterial classification in the described microbial inoculum respectively from Radix Glycyrrhizae factory waste water, pond sludge, glycyrrhiza residue and pedotheque separation and purification go out bacterium, actinomyces, saccharomycete, filamentous fungi; Adopt biotechnology, carry out multiple bacteria compound fermentation, obtained Mixed Microbes, utilize Mixed Microbes that glycyrrhiza residue is fermented then, can obtain glycyrrhiza residue roughage.That is to say, the multiple microorganism that artificial separation and purification is obtained fits together, give conditional stimulus such as appropriate nutrition, temperature, humidity, make multiple microorganism in the micro-ecological environment of glycyrrhiza residue, utilize correlation symplastic growth, breeding between the microorganism.Because microbial growth produces the effect of a large amount of microbial enzymes, thereby the physics of glycyrrhiza residue, chemical property are changed, improved its palatability, digestibility and nutritive value.Owing in glycyrrhiza residue, contain a spot of extract of licorice root, composition such as glycyrrhizin and enoxolone, these compositions ease pain to cough-relieving, anti-inflammation and disease-resistant change all have good effect, therefore, utilize the hay slag to prepare sheep husbandry by biofermentation technique, cattle-raisings etc. are fattened the handling problem that roughage not only solved glycyrrhiza residue to turn waste into wealth, also solved the problem of energy feed resource shortage simultaneously, increased the efficient utilization of limited resources, empirical tests also has tangible medical health effect to the object of feeding, according to observations, the incidence of disease of " the OK a karaoke club Ku Er lamb " fed with this feed is starkly lower than " the OK a karaoke club Ku Er lamb " that general roughage is fed.
Licorice dreg fodder technology of the present invention, this feed is to be raw material with the glycyrrhiza residue, with wheat bran, corn flour, cottonseed cake, sodium chloride, fish meal, brown sugar, to add the urea, the lactic acid that account for the raw material total amount in addition be that auxiliary material mixes, and makes glycyrrhiza residue roughage with the bacteria fermentation of preparation or with commercially available commodity starfish medicament fermentation or with commercially available commodity FP4 medicament fermentation respectively; The preparation microbial inoculum in bacterial classification be respectively from Radix Glycyrrhizae factory waste water, pond sludge, glycyrrhiza residue and pedotheque separation and purification go out bacterium, actinomyces, saccharomycete, filamentous fungi as the primary election bacterium; Bacterium is lactic acid bacteria, cellulose-decomposing bacterium; Filamentous fungi is Mucor, head mold, mould, aspergillus, wooden mould.
Be that raw material is made glycyrrhiza residue roughage with the preparation bacteria fermentation and followed these steps to carry out with the glycyrrhiza residue:
At first prepare culture medium
Dull and stereotyped dibbling culture medium: with glycyrrhiza residue 1-9 gram, agar 2 grams, water 100 grams, PH prepares back packing triangular flask naturally, and wrapping was with 0.11 MPa sterilization 30 minutes.
Single bacteria solid fermentation culture medium: glycyrrhiza residue 100%, 0.11 MPa/hour, material-water ratio 1: 1.1, the PH nature was with 0.11 MPa sterilization 30 minutes.
Solid fermentation culture medium: glycyrrhiza residue 80%, wheat bran 10%, corn flour 5%, cottonseed cake 1%, sodium chloride 1%, fish meal 1%, brown sugar 2%, added urea 0.3%, lactic acid 0.5%, material-water ratio 1: 1.1 in addition, pH value nature, 121 ℃ of high-temperature sterilizations 2 hours.
Liquid fermentation medium: glycyrrhiza residue 100 grams, brown sugar 20 grams, magnesium sulfate 2 grams, potassium dihydrogen phosphate 2 grams, yeast extract 1 gram, ammonium sulfate 5 grams, 1000 milliliters in water, PH nature, 0.11 MPa were sterilized 30 minutes.
The preparation microbial inoculum
At first prepare Mixed Microbes: bacterium, actinomyces, saccharomycete, filamentous fungi that separation and purification in the sample goes out are measured the reservation cellulose-decomposing bacterium by cellulase activity, keep lactic acid bacteria by the lactic acid production analysis, relatively keep saccharomycete by proliferative ability, keep actinomyces according to the actinomycetic speed of growth, keep mould according to qualifications according to the mycelial growth situation, carry out multiple bacteria compound fermentation then, can obtain Mixed Microbes.
The liquid bacterial agent preparation
Activate on the sterile test tube inclined-plane that the test tube slant mixed bacteria switching that keeps is fresh, be made into bacterium liquid mixed in equal amounts with sterilized water behind the full packages, be inoculated in the fresh aseptic liquid nutrient medium, shook bottle shaken cultivation 3 days under 25 ℃, packing is preserved and is promptly got liquid bacterial agent then.This liquid bacterial agent characteristic is pH value 1.0-3.0, and viable count reaches 10
10Individual/milliliter, by pure fragrance and stronger tart flavour, surperficial adularescent mycoderm.
The solid fungicide preparation
Activate on the sterile test tube inclined-plane that the test tube slant mixed bacteria switching that keeps is fresh, be made into bacterium liquid mixed in equal amounts with sterilized water behind the full packages, be inoculated in the fresh aseptic liquid nutrient medium, shook bottle shaken cultivation under 25 ℃ 3 days, be transferred to again on the sterile solid culture medium, cultivated 10 days under the 25-30 ℃ of condition, packing is preserved and is promptly got solid fungicide.This microbial inoculum characteristic, pH value 3-5, viable count is not less than 10
10Individual/the gram thousandweight, see the white hypha body between material, denseer alcohol and tartaric acid fragrance are arranged, soft, loose, moistening.
The preparation feed
Inoculate solid fermentation medium culture 3-7 days with solid mix bacterium agent or liquid mix bacterium agent in 1: 10 ratio, stand-by;
On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 75-85%, auxiliary material wheat bran 10-15%, corn flour 2-5%, cottonseed cake 0.5-1.0%, sodium chloride 0.5-1.0%, fish meal 0.5-1.0%, brown sugar 1.5-2.0%, other adds the urea 0.3-0.5% that accounts for the raw material total amount, lactic acid 0.3-0.5% mixes, fully stir, glycyrrhiza residue after stirring packed into spray the microbial inoculum for preparing in the airtight polybag and ferment, its fermentation condition is that material-water ratio is 1: 1.1, PH5-7, inoculum concentration 10-30%, cultivation temperature 25-30 ℃, incubation time 10-20 days, raw material is unsterilised, can obtain glycyrrhiza residue roughage.
With the glycyrrhiza residue is raw material, and is equipped with the fermentation of auxiliary material and commercial goods starfish medicament and makes glycyrrhiza residue roughage and press routine step and carry out:
With every bag of net weight of commercial goods starfish medicament 3 grams, handle 500 kilograms of glycyrrhiza residues for every bag:
The starfish medicament is poured in 200 ml tap waters, added 4 gram white sugar, fully dissolving is left standstill at normal temperatures and was obtained multiple living bacterial liquid in 2 hours;
Bacterium liquid after bringing back to life is poured in 1% salt solution of abundant dissolving and stirred, add 5% wheat bran and stir stand-by;
On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 75-85%, auxiliary material wheat bran 10-15%, corn flour 2-5%, cottonseed cake 0.5-1.0%, sodium chloride 0.5-1.0%, fish meal 0.5-1.0%, brown sugar 1.5-2.0%, add the urea 0.3-0.5%, the lactic acid 0.3-0.5% that account for the raw material total amount in addition and mix, fully stir, glycyrrhiza residue after stirring packed into spray the bacterium liquid for preparing in the airtight polybag and ferment, with the gravity compacting, airtight sack gets final product.
With the glycyrrhiza residue is raw material, and is equipped with the fermentation of auxiliary material and commercial goods FP4 medicament and makes glycyrrhiza residue roughage and press routine step and carry out:
With every bag of net weight of commercial goods FP4 medicament 3 grams, handle 500 kilograms of glycyrrhiza residues for every bag;
The FP4 medicament is poured in 200 ml tap waters, added 4 gram white sugar, fully dissolving is left standstill at normal temperatures and was obtained multiple living bacterial liquid in 2 hours;
Bacterium liquid after bringing back to life is poured in 1% salt solution of abundant dissolving and stirred, add 5% wheat bran and stir stand-by;
On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 75-85%, auxiliary material wheat bran 10-15%, corn flour 2-5%, cottonseed cake 0.5-1.0%, sodium chloride 0.5-1.0%, fish meal 0.5-1.0%, brown sugar 1.5-2.0%, add the urea 0.3-0.5%, the lactic acid 0.3-0.5% that account for the raw material total amount in addition and mix, fully stir, glycyrrhiza residue after stirring packed into spray the bacterium liquid for preparing in the airtight polybag and ferment, with the gravity compacting, airtight sack gets final product.
Licorice dreg fodder technology of the present invention, the bacterial classification described in this feed respectively from Radix Glycyrrhizae factory waste water, pond sludge, glycyrrhiza residue and pedotheque separation and purification go out bacterium, actinomyces, saccharomycete, filamentous fungi; The characteristic of each bacterial classification is:
Bacterium: lactic acid bacteria: the cell ovoid, in pairs many or one-tenth short chain shape, gram-positive bacterium, bacterium colony is smooth, and is little white, glossy.
Cellulose-decomposing bacterium: rod bacterium, gemma are held life partially, Gram-positive, and growth presents the brown spot on the filter paper.
Actinomyces: flourishing mycelium is arranged, and mycelium divides plate, no barrier film, and the bacterium colony front is a purple, and reverse side is brown, and the speed of growth is very fast.
Saccharomycete: cell sphere or oval, draw on the culture medium at Fructus Hordei Germinatus, the bacterium colony canescence, concave, the edge zigzag, smooth surface is moistening.
Filamentous fungi: Mucor: mycelia just is a white, after transfer yellowish-brown to, the sporangiophore later stage can back branch, sporangium sphere, brown.
Head mold: high temperature resistant, growth is fast, and bacterium colony is thick close, the rhizoid brown, and stolon is short, intensive, and bacterium colony is black behind the generation spore.
Mould: conidiophore is asymmetric, and bacterium colony is green.
Aspergillus: pediculated cells, the top capsule is subsphaeroidal, on the capsule of top give birth to two-layer radial stigma, it is green that bacterium colony is.
Wood is mould: Trichoderma viride.
Licorice dreg fodder technology of the present invention is its nutritional labeling 1 table 1 (%) that sees the following form after tested
The present invention partly substitutes cotton seed hulls with licorice dreg fodder and fattens, and the contrast test phase is 31 days, and scale is 1000 sheep, averages, and 2 tables 2 see the following form
| Group | The 1st group | The 2nd group | The 3rd group | The 4th group |
| Starting weight (Kg) | ????22.1 | ????22.75 | ????22.3 | ????22.6 |
| Fine fodder feed intake (Kg) | ????0.3 | ????0.3 | ????0.3 | ????0.3 |
| Cotton seed hull feed intake (kg) | ????0.7 | ????0.7 | ????0.7 | ????1.5 |
| Glycyrrhiza residue feed intake (Kg) | ????0.8 | ????0.8 | ????0.8 | ????0 |
| End heavy (kg) | ????25.7 | ????26.25 | ????25.8 | ????25.9 |
| Daily gain (g) | ????116.13 | ????112.90 | ????112.90 | ????106.45 |
| Feedstuff-meat ratio | ????12.92 | ????13.29 | ????13.29 | ????14.09 |
As can be seen from the table, the 2nd group of daily gain with the 3rd group is identical, and the 1st group daily gain is higher than the 2nd group, and the 2nd, 3 group daily gain is higher than the 4th group.From feedstuff-meat ratio: the 1st group is lower than the 2nd, 3 group, and the 2nd, 3 group is lower than the 4th group; The price of deed that illustrates the 1st group is higher, 4 groups the price of deed that the 2nd, 3 group the price of deed is higher than.
Embodiment 1
Be that raw material is made glycyrrhiza residue roughage with the preparation bacteria fermentation and followed these steps to carry out with the glycyrrhiza residue:
At first prepare culture medium
Dull and stereotyped dibbling culture medium: with glycyrrhiza residue 1-9 gram, agar 2 grams, water 100 grams, PH prepares back packing triangular flask naturally, and wrapping was with 0.11 MPa sterilization 30 minutes.
Single bacteria solid fermentation culture medium: glycyrrhiza residue 100%, 0.11 MPa/hour, material-water ratio 1: 1.1, the PH nature was with 0.11 MPa sterilization 30 minutes.
Solid fermentation culture medium: glycyrrhiza residue 80%, wheat bran 10%, corn flour 5%, cottonseed cake 1%, sodium chloride 1%, fish meal 1%, brown sugar 2%, added urea 0.3%, lactic acid 0.5%, material-water ratio 1: 1.1 in addition, preparation microbial inoculum pH value nature, 121 ℃ of high-temperature sterilizations 2 hours.
Liquid fermentation medium: glycyrrhiza residue 100 grams, brown sugar 20 grams, magnesium sulfate 2 grams, potassium dihydrogen phosphate 2 grams, yeast extract 1 gram, ammonium sulfate 5 grams, 1000 milliliters in water, PH nature, 0.11 MPa were sterilized 30 minutes.
The preparation microbial inoculum
At first prepare Mixed Microbes: bacterium, actinomyces, saccharomycete, filamentous fungi that separation and purification in the sample goes out are measured the reservation cellulose-decomposing bacterium by cellulase activity, keep lactic acid bacteria by the lactic acid production analysis, relatively keep saccharomycete by proliferative ability, keep actinomyces according to the actinomycetic speed of growth, keep mould according to qualifications according to the mycelial growth situation, carry out multiple bacteria compound fermentation then, can obtain Mixed Microbes.
The liquid bacterial agent preparation
Activate on the sterile test tube inclined-plane that the test tube slant mixed bacteria switching that keeps is fresh, be made into bacterium liquid mixed in equal amounts with sterilized water behind the full packages, be inoculated in the fresh aseptic liquid nutrient medium, shook bottle shaken cultivation 3 days under 25 ℃, packing is preserved and is promptly got liquid bacterial agent then.This liquid bacterial agent characteristic is pH value 1.0-3.0, and viable count reaches 10
10Individual/milliliter, by pure fragrance and stronger tart flavour, surperficial adularescent mycoderm.
The solid fungicide preparation
Activate on the sterile test tube inclined-plane that the test tube slant mixed bacteria switching that keeps is fresh, be made into bacterium liquid mixed in equal amounts with sterilized water behind the full packages, be inoculated in the fresh aseptic liquid nutrient medium, shook bottle shaken cultivation under 25 ℃ 3 days, be transferred to again on the sterile solid culture medium, cultivated 10 days under the 25-30 ℃ of condition, packing is preserved and is promptly got solid fungicide.This microbial inoculum characteristic, pH value 3-5, viable count is not less than 10
10Individual/the gram thousandweight, see the white hypha body between material, denseer alcohol and tartaric acid fragrance are arranged, soft, loose, moistening.
The preparation feed
Inoculate solid fermentation medium culture 3-7 days with solid mix bacterium agent or liquid mix bacterium agent in 1: 10 ratio, stand-by; On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 75%, auxiliary material wheat bran 15%, corn flour 5%, cottonseed cake 1.0%, sodium chloride 1.0%, fish meal 1.0%, brown sugar 2.0%, other adds the urea 0.3% that accounts for the raw material total amount, lactic acid 0.5% mixes, fully stir, glycyrrhiza residue after stirring packed into spray the microbial inoculum for preparing in the airtight polybag and ferment, its fermentation condition is that material-water ratio is 1: 1.1, PH5-7, inoculum concentration 10-30%, cultivation temperature 25-30 ℃, incubation time 10-20 days, raw material is unsterilised, can obtain glycyrrhiza residue roughage.
Embodiment 2
Be that the fermentation of raw material and commercial goods starfish medicament is made glycyrrhiza residue roughage and pressed routine step and carry out with the glycyrrhiza residue:
With every bag of net weight of commercial goods starfish medicament 3 grams, handle 500 kilograms of glycyrrhiza residues for every bag;
The starfish medicament is poured in 200 ml tap waters, added 4 gram white sugar, fully dissolving is left standstill at normal temperatures and was obtained multiple living bacterial liquid in 2 hours;
Bacterium liquid after bringing back to life is poured in 1% salt solution of abundant dissolving and stirred, add 5% wheat bran and stir stand-by;
On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 80%, auxiliary material wheat bran 12%, corn flour 4%, cottonseed cake 0.7%, sodium chloride 0.8%, fish meal 0.80%, brown sugar 1.7%, add the urea 0.4%, the lactic acid 0.4% that account for the raw material total amount in addition and mix, fully stir, glycyrrhiza residue after stirring packed into spray the bacterium liquid for preparing in the airtight polybag and ferment, with the gravity compacting, airtight sack gets final product.
Embodiment 3
Be that the fermentation of raw material and commercial goods FP4 medicament is made glycyrrhiza residue roughage and pressed routine step and carry out with the glycyrrhiza residue:
With every bag of net weight of commercial goods FP4 medicament 3 grams, handle 500 kilograms of glycyrrhiza residues for every bag;
The FP4 medicament is poured in 200 ml tap waters, added 4 gram white sugar, fully dissolving is left standstill at normal temperatures and was obtained multiple living bacterial liquid in 2 hours; Bacterium liquid after bringing back to life is poured in 1% salt solution of abundant dissolving and stirred, add 5% wheat bran and stir stand-by;
On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 85%, auxiliary material wheat bran 10%, corn flour 2%, cottonseed cake 0.5%, sodium chloride 0.5%, fish meal 0.5%, brown sugar 1.5%, add the urea 0.5%, the lactic acid 0.3% that account for the raw material total amount in addition and mix, fully stir, glycyrrhiza residue after stirring packed into spray the bacterium liquid for preparing in the airtight polybag and ferment, with the gravity compacting, airtight sack gets final product.
Claims (4)
1, a kind of licorice dreg fodder technology, it is characterized in that this feed is is raw material with the glycyrrhiza residue, with wheat bran, corn flour, cottonseed cake, sodium chloride, fish meal, brown sugar, to add the urea, the lactic acid that account for the raw material total amount in addition be that auxiliary material mixes, and makes glycyrrhiza residue roughage with the bacteria fermentation of preparation or with commercially available commodity starfish medicament fermentation or with commercially available commodity FP4 medicament fermentation respectively; The preparation microbial inoculum in bacterial classification be respectively from Radix Glycyrrhizae factory waste water, pond sludge, glycyrrhiza residue and pedotheque separation and purification go out bacterium, actinomyces, saccharomycete, filamentous fungi as the primary election bacterium; Bacterium is lactic acid bacteria, cellulose-decomposing bacterium; Filamentous fungi is Mucor, head mold, mould, aspergillus, wooden mould.
2, the preparation method of licorice dreg fodder technology according to claim 1 is characterized in that with the glycyrrhiza residue that to be raw material make glycyrrhiza residue roughage with the preparation bacteria fermentation to follow these steps to carry out:
At first prepare culture medium
Dull and stereotyped dibbling culture medium: with glycyrrhiza residue 1-9 gram, agar 2 grams, water 100 grams, PH prepares back packing triangular flask naturally, and wrapping was with 0.11 MPa sterilization 30 minutes.
Single bacteria solid fermentation culture medium: glycyrrhiza residue 100%, 0.11 MPa/hour, material-water ratio 1: 1.1, the PH nature was with 0.11 MPa sterilization 30 minutes.
Solid fermentation culture medium: glycyrrhiza residue 80%, wheat bran 10%, corn flour 5%, cottonseed cake 1%, sodium chloride 1%, fish meal 1%, brown sugar 2%, added urea 0.3%, lactic acid 0.5%, material-water ratio 1: 1.1 in addition, pH value nature, 121 ℃ of high-temperature sterilizations 2 hours.
Liquid fermentation medium: glycyrrhiza residue 100 grams, brown sugar 20 grams, magnesium sulfate 2 grams, potassium dihydrogen phosphate 2 grams, yeast extract 1 gram, ammonium sulfate 5 grams, 1000 milliliters in water, PH nature, 0.11 MPa were sterilized 30 minutes.
The preparation microbial inoculum
At first prepare Mixed Microbes: bacterium, actinomyces, saccharomycete, filamentous fungi that separation and purification in the sample goes out are measured the reservation cellulose-decomposing bacterium by cellulase activity, keep lactic acid bacteria by the lactic acid production analysis, relatively keep saccharomycete by proliferative ability, keep actinomyces according to the actinomycetic speed of growth, keep mould according to qualifications according to the mycelial growth situation, carry out multiple bacteria compound fermentation then, can obtain Mixed Microbes.
The liquid bacterial agent preparation
Activate on the sterile test tube inclined-plane that the test tube slant mixed bacteria switching that keeps is fresh, be made into bacterium liquid mixed in equal amounts with sterilized water behind the full packages, be inoculated in the fresh aseptic liquid nutrient medium, shook bottle shaken cultivation 3 days under 25 ℃, packing is preserved and is promptly got liquid bacterial agent then.This liquid bacterial agent characteristic is pH value 1.0-3.0, and viable count reaches 10
10Individual/milliliter, by pure fragrance and stronger tart flavour, surperficial adularescent mycoderm.
The solid fungicide preparation
Activate on the sterile test tube inclined-plane that the test tube slant mixed bacteria switching that keeps is fresh, be made into bacterium liquid mixed in equal amounts with sterilized water behind the full packages, be inoculated in the fresh aseptic liquid nutrient medium, shook bottle shaken cultivation under 25 ℃ 3 days, be transferred to again on the sterile solid culture medium, cultivated 10 days under the 25-30 ℃ of condition, packing is preserved and is promptly got solid fungicide.This microbial inoculum characteristic, pH value 3-5, viable count is not less than 10
10Individual/the gram thousandweight, see the white hypha body between material, denseer alcohol and tartaric acid fragrance are arranged, soft, loose, moistening.
The preparation feed
Inoculate solid fermentation medium culture 3-7 days with solid mix bacterium agent or liquid mix bacterium agent in 1: 10 ratio, stand-by;
On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 75-859%, auxiliary material wheat bran 10-15%, corn flour 2-5%, cottonseed cake 0.5-1.0%, sodium chloride 0.5-1.0%, fish meal 0.5-1.0%, brown sugar 1.5-2.0%, other adds the urea 0.3-0.5% that accounts for the raw material total amount, lactic acid 0.3-0.5% mixes, fully stir, glycyrrhiza residue after stirring packed into spray the microbial inoculum for preparing in the airtight polybag and ferment, its fermentation condition is that material-water ratio is 1: 1.1, PH5-7, inoculum concentration 10-30%, cultivation temperature 25-30 ℃, incubation time 10-20 days, raw material is unsterilised, can obtain glycyrrhiza residue roughage.
3, the preparation method of licorice dreg fodder technology according to claim 1 is characterized in that with the glycyrrhiza residue being raw material, and is equipped with the fermentation of auxiliary material and commercial goods starfish medicament and makes glycyrrhiza residue roughage and press routine step and carry out:
With every bag of net weight of commercial goods starfish medicament 3 grams, handle 500 kilograms of glycyrrhiza residues for every bag;
The starfish medicament is poured in 200 ml tap waters, added 4 gram white sugar, fully dissolving is left standstill at normal temperatures and was obtained multiple living bacterial liquid in 2 hours;
Bacterium liquid after bringing back to life is poured in 1% salt solution of abundant dissolving and stirred, add 5% wheat bran and stir stand-by;
On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 75-85%, auxiliary material wheat bran 10-15%, corn flour 2-5%, cottonseed cake 0.5-1.0%, sodium chloride 0.5-1.0%, fish meal 0.5-1.0%, brown sugar 1.5-2.0%, add the urea 0.3-0.5%, the lactic acid 0.3-0.5% that account for the raw material total amount in addition and mix, fully stir, glycyrrhiza residue after stirring packed into spray the bacterium liquid for preparing in the airtight polybag and ferment, with the gravity compacting, airtight sack gets final product.
4, the preparation method of licorice dreg fodder technology according to claim 1 is characterized in that with the glycyrrhiza residue being raw material, and is equipped with the fermentation of auxiliary material and commercial goods starfish medicament and makes glycyrrhiza residue roughage and press routine step and carry out:
With every bag of net weight of commercial goods FP4 medicament 3 grams, handle 500 kilograms of glycyrrhiza residues for every bag;
The FP4 medicament is poured in 200 ml tap waters, added 4 gram white sugar, fully dissolving is left standstill at normal temperatures and was obtained multiple living bacterial liquid in 2 hours;
Bacterium liquid after bringing back to life is poured in 1% salt solution of abundant dissolving and stirred, add 5% wheat bran and stir stand-by;
On the glycyrrhiza residue of having pulverized, spray the moisture of 1.4 times of glycyrrhiza residue weight, then with raw material glycyrrhiza residue 75-85%, auxiliary material wheat bran 10-15%, corn flour 2-5%, cottonseed cake 0.5-1.09%, sodium chloride 0.5-1.0%, fish meal 0.5-1.0%, brown sugar 1.5-2.0%, add the urea 0.3-0.5%, the lactic acid 0.3-0.5% that account for the raw material total amount in addition and mix, fully stir, glycyrrhiza residue after stirring packed into spray the bacterium liquid for preparing in the airtight polybag and ferment, with the gravity compacting, airtight sack gets final product.
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| CN01117626A CN1322483A (en) | 2001-04-29 | 2001-04-29 | Licorice dreg fodder technology |
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| CN01117626A CN1322483A (en) | 2001-04-29 | 2001-04-29 | Licorice dreg fodder technology |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202389A (en) * | 2013-04-25 | 2013-07-17 | 叶文明 | Preparation method of licorice residue roughage |
| CN103224887A (en) * | 2013-05-07 | 2013-07-31 | 山东省环境保护科学研究设计院 | Preparation method of microbial nutrition agent for wastewater biochemical treatment |
| CN103229890A (en) * | 2013-04-25 | 2013-08-07 | 叶文明 | Preparation method of mixed Chinese medicine residue feed |
| CN104605141A (en) * | 2015-02-18 | 2015-05-13 | 骆金福 | Ecologic feed leavening agent synthesized by using tree seeds |
| CN105028908A (en) * | 2015-08-11 | 2015-11-11 | 新疆石河子职业技术学院 | Licorice root residue protein feed and preparation method thereof |
| CN105850883A (en) * | 2016-04-27 | 2016-08-17 | 连云港恒图农牧开发有限公司 | Method for establishing mutton sheep circulating breeding ecological system through biological bacteria |
| CN107502553A (en) * | 2017-07-11 | 2017-12-22 | 南京中医药大学 | A kind of cellulase producing bacteria for being resistant to liquorice dregs and the method applied to liquorice dregs cellulase-producing |
-
2001
- 2001-04-29 CN CN01117626A patent/CN1322483A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202389A (en) * | 2013-04-25 | 2013-07-17 | 叶文明 | Preparation method of licorice residue roughage |
| CN103229890A (en) * | 2013-04-25 | 2013-08-07 | 叶文明 | Preparation method of mixed Chinese medicine residue feed |
| CN103224887A (en) * | 2013-05-07 | 2013-07-31 | 山东省环境保护科学研究设计院 | Preparation method of microbial nutrition agent for wastewater biochemical treatment |
| CN103224887B (en) * | 2013-05-07 | 2014-08-27 | 山东省环境保护科学研究设计院 | Preparation method of microbial nutrition agent for wastewater biochemical treatment |
| CN104605141A (en) * | 2015-02-18 | 2015-05-13 | 骆金福 | Ecologic feed leavening agent synthesized by using tree seeds |
| CN105028908A (en) * | 2015-08-11 | 2015-11-11 | 新疆石河子职业技术学院 | Licorice root residue protein feed and preparation method thereof |
| CN105850883A (en) * | 2016-04-27 | 2016-08-17 | 连云港恒图农牧开发有限公司 | Method for establishing mutton sheep circulating breeding ecological system through biological bacteria |
| CN107502553A (en) * | 2017-07-11 | 2017-12-22 | 南京中医药大学 | A kind of cellulase producing bacteria for being resistant to liquorice dregs and the method applied to liquorice dregs cellulase-producing |
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