CN1314417A - Process for separating and purifying lentinan - Google Patents
Process for separating and purifying lentinan Download PDFInfo
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- CN1314417A CN1314417A CN 00114433 CN00114433A CN1314417A CN 1314417 A CN1314417 A CN 1314417A CN 00114433 CN00114433 CN 00114433 CN 00114433 A CN00114433 A CN 00114433A CN 1314417 A CN1314417 A CN 1314417A
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Abstract
The process of purifying lentinan, unlike traditional processes, eliminates pigment and small molecular matters while eliminating protein. The technological process of the present invention includes chloroform and butanol extraction to eliminate hybrid protein, as well as ultrafiltration, ion exchange column chromatography and molecular sieve column chromatogrphy for further separation and purification. The obtained lentinan is simple and has purity over 90 %.
Description
The present invention relates to the bioseparation purification technique, specifically about the technology of Lentinan separation and purification.
Before the present invention proposes, the method of traditional separation and purification Lentinan, only on technology, take method for removing protein, do not remove pigment and small-molecule substance pointedly, thereby its purity generally can only reach 30%-40% behind the purifying, at most also can only reach about 70%, only can make general oral preparations product.
The objective of the invention is the Lentinus Edodes fungus Crude polysaccharides is carried out " three take off " (deproteinated, decolouring, desalination) handles, and carry out molecular weight and hold back ultrafiltration and column chromatography purification, to reach the purity that improves Lentinan, make it can be used to prepare the bulk drug of several formulations such as Lentinan tablet, capsule, injection.
Realizing concrete processing method of the present invention, is from the mycelium that the Lentinus Edodes fungus deep layer is cultivated, and extracts biological activity Lentinus Edodes fungus Crude polysaccharides, and it is carried out deproteinated, desalination, and depigmentation is handled and column chromatography purification.Concrete processing step: 1. deproteinated: get 1 part of Lentinus Edodes fungus Crude polysaccharides, add water 2000ml behind 90～100 ℃ of heating 30min, the centrifugal supernatant of collecting of 3000rpm, add equal amounts of chloroform: propyl carbinol (1: 0.2～1: 0.25), extraction foreign protein 5～10 times, 3000rpm centrifuging and taking upper strata, most of foreign protein is removed.2. molecular weight is held back ultrafiltration: (1) desalination, and with the ultra-filtration membrane ultrafiltration desalination and the removal small molecular weight impurity of molecular weight cut-off (MW50,000).(2) remove macromole impurity, with molecular weight cut-off (MW100,000) macromole impurity is removed in ultra-filtration membrane ultrafiltration, regulates PH5.8～PH6.5.3. column chromatography three-step approach purifying: (1) anion exchange chromatography (DE-52), with PH5.8～PH6.5,0.01MPBS damping fluid balance DE52 post, applied sample amount 40ML～80ML, flow velocity 1～3ml/min surveys total reducing sugar, eluting peak is the polysaccharide peak, and pigment and partial impurities are adsorbed in separated removal in the exchange column; (2) cation exchange column chromatography, with PH4.8～PH5.5,0.01MPBS damping fluid balance CM52 post further separates, and flow velocity 0.1～0.3ml/min surveys total reducing sugar, obtains 6 peaks, and peak 6 is required polysaccharide peak; (3) molecular sieve column chromatography, after behind the peak 6 concentrated 5～10 times, last Sephedax post, flow velocity 0.1～0.3ml/min surveys total reducing sugar, obtains 2 peaks, and peak 1 is the Lentinan peak after the separation and purification, and detecting through high performance liquid phase is simple spike; 4. dry, the peak 1 behind the mistake sephedax G-100 column chromatography after lyophilize, is the dried powder of Lentinan refining pure product.Detect through high performance liquid phase, it is the single polysaccharide composition, and purity reaches more than 90%.
The present invention compares with traditional only single deproteinated of separation and purification Lentinan, has embodied the superiority and the operability of its technology fully.Its process practice proves, the Lentinan that this method is obtained, major ingredient are Streptococel, and its polysaccharide part is mainly based on seminose, has effects such as antiviral, antitumor, bacterial-infection resisting, inducement interferon and enhancing body immunity.
Below in conjunction with drawings and Examples, further describe the present invention:
Accompanying drawing 1 of the present invention is the process flow sheet of separation and purification Lentinan, and accompanying drawing 2 is anion exchange chromatography collection of illustrative plates, and accompanying drawing 3 is cation exchange column chromatography collection of illustrative plates, and accompanying drawing 4 is that molecular sieve column is analysed collection of illustrative plates, and accompanying drawing 5 is that high performance liquid phase detects collection of illustrative plates.
Embodiment implementation and operation step (seeing accompanying drawing 1) is deproteinated 1.
Lentinus Edodes fungus Crude polysaccharides 10g adds water 2000ml behind 90 ℃ of heating 30min, and the centrifugal supernatant of collecting of 3000rpm adds equal amounts of chloroform: propyl carbinol (1: 0.22) extraction foreign protein 7 times, and the centrifugal upper strata of collecting of 3000rpm, most of foreign protein is removed.2. ultrafiltration
1. desalination (and removing small molecular weight impurity)
Ultra-filtration membrane ultrafiltration desalination and removal small molecular weight impurity with molecular weight cut-off (MW50,000).
2. remove macromole impurity
Remove macromole impurity with the ultra-filtration membrane ultrafiltration of holding back molecule (MW100,000), regulate PH6.0.3. column chromatography purification (three-step approach)
1. anion exchange chromatography (DE-52)
Use PH6.0, and 0.01MPBS damping fluid balance DE52 post (4 * 20cm), applied sample amount 50ml, flow velocity 2ml/min surveys total reducing sugar, and eluting peak is the polysaccharide peak, and pigment and partial impurities are adsorbed in separated removal (Fig. 2) in the exchange column
2. cation exchange column chromatography
Use PH5.0, (2 * 100cm) further separate 0.01MPBS damping fluid balance CM52 post, and flow velocity 0.2ml/min surveys total reducing sugar, obtains 6 peaks, and peak 6 is required polysaccharide peak (Fig. 3)
3. molecular sieve column chromatography
After peak 6 concentrated 5 times, (2 * 100cm), flow velocity 0.1ml/min surveyed total reducing sugar to last Sephedax G-100, obtains 2 peaks, and peak 1 is the Lentinan peak (Fig. 4) after the separation and purification, and detecting through high performance liquid phase is simple spike (Fig. 5).
4. dry
Peak 1 behind the mistake Sephedax G-100 column chromatography after lyophilize, is the dried powder of Lentinan refining pure product.Detect through high performance liquid phase, it is the single polysaccharide composition, and purity reaches more than 90%.
In a word, the invention provides a kind of new process of separation and purification Lentinan, the bulk drug that the Lentinan for preparing by the present invention can be used as several formulations uses.
Claims (3)
1. the technology of a separation and purification Lentinan which comprises at least: the Lentinus Edodes fungus Crude polysaccharides is carried out deproteinated, decolouring, desalting treatment and its molecular weight is held back ultrafiltration and column chromatography purification, final drying.
2. the technology of a kind of separation and purification Lentinan according to claim 1, it is characterized in that: (1) deproteinated: get 1 part of Lentinus Edodes fungus Crude polysaccharides, add water 2000ml behind 90～100 ℃ of heating 30min, the centrifugal supernatant of collecting of 3000rpm, add equal amounts of chloroform: propyl carbinol (1: 0.2～1: 0.25), extraction foreign protein 5～10 times, 3000rpm centrifuging and taking upper strata, most of foreign protein is removed.(2) molecular weight is held back ultrafiltration: a. desalination, and with the ultra-filtration membrane ultrafiltration desalination and the removal small molecular weight impurity of molecular weight cut-off (MW50,000).B. remove macromole impurity, remove macromole impurity, regulate PH5.8～PH6.5 with the ultra-filtration membrane ultrafiltration of molecular weight cut-off (MW100,000).
3. the technology of a kind of separation and purification Lentinan according to claim 1, it is characterized in that column chromatography three-step approach purifying: (1) anion exchange chromatography (DE-52), with PH5.8～PH6.5,0.01MPBS damping fluid balance DE52 post, applied sample amount 40ML～80ML, flow velocity 1～3ml/min surveys total reducing sugar, eluting peak is the polysaccharide peak, and pigment and partial impurities are adsorbed in separated removal in the exchange column; (2) cation exchange column chromatography, with PH4.8～PH5.5,0.01MPBS damping fluid balance CM52 post further separates, and flow velocity 0.1～0.3ml/min surveys total reducing sugar, obtains 6 peaks, and peak 6 is required polysaccharide peak; (3) molecular sieve column chromatography, peak 6 concentrated 5～10 times after, last sephedax post, flow velocity 0.1～0.3ml/min surveys total reducing sugar, obtains 2 peaks, peak 1 is the Lentinan peak after the separation and purification, detecting through high performance liquid phase is simple spike.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB001144332A CN1143866C (en) | 2000-03-20 | 2000-03-20 | Process for separating and purifying lentinan |
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| CNB001144332A CN1143866C (en) | 2000-03-20 | 2000-03-20 | Process for separating and purifying lentinan |
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| CN1314417A true CN1314417A (en) | 2001-09-26 |
| CN1143866C CN1143866C (en) | 2004-03-31 |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1296389C (en) * | 2004-09-14 | 2007-01-24 | 武汉大学 | Three spiral lentinan with anti-cancer activity and its preparation and use |
| CN1314711C (en) * | 2004-03-27 | 2007-05-09 | 中国科学院水生生物研究所 | Method for extracting Gexianmi amylose from nostoc |
| CN1322015C (en) * | 2004-03-27 | 2007-06-20 | 中国科学院水生生物研究所 | Method for extracting amylose of desert algae |
| CN100354310C (en) * | 2005-10-11 | 2007-12-12 | 大连轻工业学院 | Mussel polysacharide and its preparing method |
| US7514085B2 (en) | 2004-07-16 | 2009-04-07 | Medimush A/S | Immune modulating compounds from fungi |
| CN101265303B (en) * | 2008-04-24 | 2010-06-02 | 吉林大学 | Method for extracting lentinan by using discarded mushroom culture medium as raw material |
| CN101278724B (en) * | 2008-01-08 | 2010-11-10 | 山西大学 | Method for sequentially extracting effective ingredients from red date |
| CN102070723A (en) * | 2010-12-03 | 2011-05-25 | 曹庸 | Agaricus bisporus polysaccharide with immunological activity as well as preparation method and application of agaricus bisporus polysaccharide |
| CN101161112B (en) * | 2006-10-10 | 2011-11-23 | 上海慈瑞医药科技有限公司 | A method for separating and purifying lentinan |
| CN101724088B (en) * | 2009-11-24 | 2012-01-25 | 浙江工业大学 | Method for removing proteins and pigments in ganoderma lucidum crude polysaccharide |
| US8758768B2 (en) | 2001-09-03 | 2014-06-24 | Glycanova As | Process for production of fungal extracellular immune stimulating compounds |
| US9072776B2 (en) | 2005-06-15 | 2015-07-07 | Glycanova As | Anti-cancer combination treatment and kit-of-parts |
| CN109001198A (en) * | 2018-09-03 | 2018-12-14 | 艾美卫信生物药业（浙江）有限公司 | The method that the NaTDC precipitation method detect free polysaccharide in Hib combined vaccine |
| CN110483653A (en) * | 2019-07-16 | 2019-11-22 | 南昌大学 | A kind of preparation method and product and application with immunocompetent lentinan component |
| CN110551230A (en) * | 2019-09-21 | 2019-12-10 | 天津赛诺制药有限公司 | Preparation method of astragalus polysaccharide |
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2000
- 2000-03-20 CN CNB001144332A patent/CN1143866C/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8758768B2 (en) | 2001-09-03 | 2014-06-24 | Glycanova As | Process for production of fungal extracellular immune stimulating compounds |
| US10471135B2 (en) | 2001-09-03 | 2019-11-12 | Glycanova As | Production of fungal extracellular immune stimulating compounds |
| US9249438B2 (en) | 2001-09-03 | 2016-02-02 | Glycanova As | Production of fungal extracellular immune stimulating compounds |
| CN1314711C (en) * | 2004-03-27 | 2007-05-09 | 中国科学院水生生物研究所 | Method for extracting Gexianmi amylose from nostoc |
| CN1322015C (en) * | 2004-03-27 | 2007-06-20 | 中国科学院水生生物研究所 | Method for extracting amylose of desert algae |
| US7514085B2 (en) | 2004-07-16 | 2009-04-07 | Medimush A/S | Immune modulating compounds from fungi |
| US7682615B2 (en) | 2004-07-16 | 2010-03-23 | Beka Holding As | Immune modulating compounds from fungi |
| CN1296389C (en) * | 2004-09-14 | 2007-01-24 | 武汉大学 | Three spiral lentinan with anti-cancer activity and its preparation and use |
| US9072776B2 (en) | 2005-06-15 | 2015-07-07 | Glycanova As | Anti-cancer combination treatment and kit-of-parts |
| CN100354310C (en) * | 2005-10-11 | 2007-12-12 | 大连轻工业学院 | Mussel polysacharide and its preparing method |
| CN101161112B (en) * | 2006-10-10 | 2011-11-23 | 上海慈瑞医药科技有限公司 | A method for separating and purifying lentinan |
| CN101278724B (en) * | 2008-01-08 | 2010-11-10 | 山西大学 | Method for sequentially extracting effective ingredients from red date |
| CN101265303B (en) * | 2008-04-24 | 2010-06-02 | 吉林大学 | Method for extracting lentinan by using discarded mushroom culture medium as raw material |
| CN101724088B (en) * | 2009-11-24 | 2012-01-25 | 浙江工业大学 | Method for removing proteins and pigments in ganoderma lucidum crude polysaccharide |
| CN102070723A (en) * | 2010-12-03 | 2011-05-25 | 曹庸 | Agaricus bisporus polysaccharide with immunological activity as well as preparation method and application of agaricus bisporus polysaccharide |
| CN109001198A (en) * | 2018-09-03 | 2018-12-14 | 艾美卫信生物药业（浙江）有限公司 | The method that the NaTDC precipitation method detect free polysaccharide in Hib combined vaccine |
| CN110483653A (en) * | 2019-07-16 | 2019-11-22 | 南昌大学 | A kind of preparation method and product and application with immunocompetent lentinan component |
| CN110483653B (en) * | 2019-07-16 | 2022-03-18 | 南昌大学 | Preparation method of lentinan component with immunocompetence, product and application |
| CN110551230A (en) * | 2019-09-21 | 2019-12-10 | 天津赛诺制药有限公司 | Preparation method of astragalus polysaccharide |
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| CN1143866C (en) | 2004-03-31 |
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