CN101029077A - Method for purifying gene-recombinant insulin precursor - Google Patents
Method for purifying gene-recombinant insulin precursor Download PDFInfo
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- CN101029077A CN101029077A CN 200710026682 CN200710026682A CN101029077A CN 101029077 A CN101029077 A CN 101029077A CN 200710026682 CN200710026682 CN 200710026682 CN 200710026682 A CN200710026682 A CN 200710026682A CN 101029077 A CN101029077 A CN 101029077A
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- insulin precursor
- recombinant insulin
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Abstract
Description
Sample | Light absorption value A | Purity/% | Concentration/mg/ml | Liquor capacity/ml | Gene-recombinant insulin precursor amount/g | The rate of recovery/% |
The collection liquid that the centrifuged supernatant that the centrifuged supernatant that does not add active carbon adds active carbon is crossed activated-charcoal column is crossed the collection liquid ultrafiltration concentration liquid of cation chromatography post | 1.452 0.940 0.341 0.083 0.012 | 63.85 75.88 88.17 96.30 97.03 | 1.20 1.17 0.91 4.90 10.7 | 1000 1000 1200 220 100 | 1.20. 1.17 1.10 1.08 1.07 | / 97.75 93.64 98.65 98.92 |
Final purity 97.03% total yield 89.16% |
Claims (6)
Priority Applications (1)
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CN2007100266823A CN101029077B (en) | 2007-02-02 | 2007-02-02 | Method for purifying gene-recombinant insulin precursor |
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CN2007100266823A CN101029077B (en) | 2007-02-02 | 2007-02-02 | Method for purifying gene-recombinant insulin precursor |
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CN101029077A true CN101029077A (en) | 2007-09-05 |
CN101029077B CN101029077B (en) | 2010-09-29 |
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CN2007100266823A Active CN101029077B (en) | 2007-02-02 | 2007-02-02 | Method for purifying gene-recombinant insulin precursor |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102532257A (en) * | 2010-12-13 | 2012-07-04 | 鲁南制药集团股份有限公司 | Method for purifying recombinant human proinsulin |
US8440878B2 (en) | 2008-02-04 | 2013-05-14 | Ben-Gurion University Of The Negev Research And Development Authority | Insulin-like gene in prawns and uses thereof |
CN103145829A (en) * | 2013-03-29 | 2013-06-12 | 江苏诺泰制药有限公司 | Purification method of insulin detemir |
CN101981046B (en) * | 2008-02-04 | 2015-01-07 | 内盖夫研究与发展部本-古里安大学 | Insulin-like gene in prawns and uses thereof |
CN105153294A (en) * | 2015-08-31 | 2015-12-16 | 济南康和医药科技有限公司 | Recombinant insulin and insulin analogue precursor purification method |
CN107383161A (en) * | 2012-06-29 | 2017-11-24 | Emd密理博公司 | The purifying of biomolecule |
CN112341535A (en) * | 2019-08-07 | 2021-02-09 | 中国科学院大连化学物理研究所 | Method for preparing insulin by separation and purification through ion exchange chromatography |
CN113563413A (en) * | 2021-08-17 | 2021-10-29 | 汉肽生物医药集团有限公司 | Method for removing pigment in high-density fermentation process of pichia pastoris |
WO2023154467A1 (en) * | 2022-02-11 | 2023-08-17 | Clara Foods Co. | Protein compositions and consumable products thereof |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100460508C (en) * | 2004-11-03 | 2009-02-11 | 马延高 | Secretory expression for human insulin gene in methyl alcohol yeast |
CN1854299A (en) * | 2005-04-29 | 2006-11-01 | 上海新生源医药研究有限公司 | Production of recombinant insulinum primary C peptide |
-
2007
- 2007-02-02 CN CN2007100266823A patent/CN101029077B/en active Active
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9049850B2 (en) | 2008-02-04 | 2015-06-09 | Ben-Gurion University Of The Negev Research And Development Authority | Insulin-like gene of prawns and uses thereof |
CN101981046B (en) * | 2008-02-04 | 2015-01-07 | 内盖夫研究与发展部本-古里安大学 | Insulin-like gene in prawns and uses thereof |
US8440878B2 (en) | 2008-02-04 | 2013-05-14 | Ben-Gurion University Of The Negev Research And Development Authority | Insulin-like gene in prawns and uses thereof |
CN102532257A (en) * | 2010-12-13 | 2012-07-04 | 鲁南制药集团股份有限公司 | Method for purifying recombinant human proinsulin |
CN102532257B (en) * | 2010-12-13 | 2015-03-18 | 鲁南制药集团股份有限公司 | Method for purifying recombinant human proinsulin |
US10865224B2 (en) | 2012-06-29 | 2020-12-15 | Emd Millipore Corporation | Purification of biological molecules |
CN107383161A (en) * | 2012-06-29 | 2017-11-24 | Emd密理博公司 | The purifying of biomolecule |
CN107383161B (en) * | 2012-06-29 | 2021-08-17 | Emd密理博公司 | Purification of biomolecules |
CN103145829B (en) * | 2013-03-29 | 2015-06-03 | 江苏诺泰制药有限公司 | Purification method of insulin detemir |
CN103145829A (en) * | 2013-03-29 | 2013-06-12 | 江苏诺泰制药有限公司 | Purification method of insulin detemir |
CN105153294A (en) * | 2015-08-31 | 2015-12-16 | 济南康和医药科技有限公司 | Recombinant insulin and insulin analogue precursor purification method |
CN112341535A (en) * | 2019-08-07 | 2021-02-09 | 中国科学院大连化学物理研究所 | Method for preparing insulin by separation and purification through ion exchange chromatography |
CN113563413A (en) * | 2021-08-17 | 2021-10-29 | 汉肽生物医药集团有限公司 | Method for removing pigment in high-density fermentation process of pichia pastoris |
WO2023154467A1 (en) * | 2022-02-11 | 2023-08-17 | Clara Foods Co. | Protein compositions and consumable products thereof |
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Publication number | Publication date |
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CN101029077B (en) | 2010-09-29 |
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