CN1312991C - Agricultural bactericidal agent - Google Patents
Agricultural bactericidal agent Download PDFInfo
- Publication number
- CN1312991C CN1312991C CNB2005100474460A CN200510047446A CN1312991C CN 1312991 C CN1312991 C CN 1312991C CN B2005100474460 A CNB2005100474460 A CN B2005100474460A CN 200510047446 A CN200510047446 A CN 200510047446A CN 1312991 C CN1312991 C CN 1312991C
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- CN
- China
- Prior art keywords
- shitosan
- chitosan
- bacterium
- base
- imino group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to bactericidal agent for agricultural diseases, particularly to chitosan schiff base bactericidal agent, wherein the chitosan schiff base is amino chitosan, propyl imino chitosan, butyl imino chitosan, phenyl imino chitosan, 2-hydroxy phenyl methyl imino chitosan, 2-hydroxy-5-chlorine phenyl methyl imino chitosan and 2-hydroxy-5-nitryl phenyl methyl imino chitosan; the optimized effective bactericidal concentration of the chitosan schiff base is from 50 ppm to 1000 ppm. The present invention has the advantage that the bactericidal agent with environmental protection and high efficiency is developed.
Description
Technical field
The present invention relates to bactericide, specifically is the bactericide of a kind of agricultural harm germ.
Background technology
The agricultural chemicals initiative is a high-tech, engineering with high investment, high risk, and a kind of new varieties of present every exploitation approximately need the 5-8 year, hundred million dollars of expensive 1-2, screen the compound about 100,000, and along with the requirement of environmental problem, these numerals also will increase.The stand-alone development new varieties of having the ability at present have only An Wante, Bayer, Meng Shan all to wait international big agricultural chemicals company.How researching and developing the novel pesticide of China, is the severe problem that faces under situation about being gradually improved along with accession to WTO and intellectual property protection.
At the actual conditions in international bactericide market and the direction of research, the situation of the active and appearance of each series bactericidal agent.Simultaneously according to our technical strength and economic strength, cooperate with the offshore company or take " me-too chemistry " to be considered to effectively formulate approach.Promptly on present existing bactericide basis, row is theoretical that existing existing group with bactericidal action is carried out the derivatization modification for thought that connects with substructure or biology etc., develops the secondary lead compound with better bactericidal activity and easier degraded.This initiative approach is with clearly defined objective, laborsaving saving money, and also opportunity of success is more.
Shitosan (Chitosan) be a kind of extensively be present in occurring in nature renewable, have no side effect, the natural glycosaminoglycan that biocompatibility and degradability are good, himself and derivative thereof have physiology, the pharmacological function character of many uniquenesses, are widely used in the multiple industry fields such as medicine, food, agricultural, daily use chemicals, environmental protection.Structure that shitosan itself is special and character have sterilization, bacteriostatic activity, and nontoxic, free of contamination characteristic, and the object that can be used as modification is in the hope of developing the secondary lead compound.Reactive group-hydroxyl on the chitosan molecule and amino can be used as the effect group when modifying, and react with other active groups, insert the antibacterial group with different activities, are the novel directions that shitosan is used on agricultural.
Qian result of study according to this, schiff bases has bactericidal effect preferably, and the amino of shitosan is modified, and obtains the chitosan schiff-base derivative and carries out sterilization experiment, has obtained the chitosan derivatives that series has fine fungistatic effect.
Summary of the invention
The objective of the invention is to develop a kind of agricultural bacteriocide of chitosan schiff-base by the inhibition effect of chitosan schiff-base to various different strains.
For realizing purpose of the present invention, technical solution of the present invention is as follows: bactericide of the present invention adopts chitosan schiff-base, and concentration is: 5ppm-1500ppm, and wherein preferred concentration is: 50ppm-1000ppm; The used chitosan schiff-base of chitosan schiff-base bactericide is second imino group shitosan, the third imino group shitosan, fourth imino group shitosan, benzene auxotox radical shitosan, 2-hydroxy benzenes auxotox radical shitosan, 2-hydroxyl-5-chlorobenzene auxotox radical shitosan or 2-hydroxyl-5-nitrobenzoyl imino group shitosan; And adopt growth rate method to carry out bacteriostatic experiment, wherein 12 kinds of common agricultural diseases are Monilinia fructicola, fruit white rot of grape bacterium, Valsa mali, tomato early blight bacterium, tomato late blight, cotton-wilt fusarium, wheat rust bacterium, Sclerotinia sclerotiorum, Rhizoctonia solani Kuhn, pear cucumerinum, bacterium of downy mildew of cucumber or corn smut bacterium.
The advantage that the present invention had:
Adopt bactericide of the present invention can avoid the pollution of chemical pesticide, can degrade at occurring in nature, simultaneously environment, shitosan has good plant regulatory function, can mutually promote with bactericidal effect, reach better bactericidal and bacteriostatic effect, strengthen the immunity of crop simultaneously.
Embodiment
Embodiment 1
1.PDA medium: get 1000mL water; The 200g potato; 20g sucrose; The 10g agar powder is configured to the PDA medium; Be divided in that every bottle of capacity is 38ml or 40ml in the triangular flask, it is standby to sterilize.
2. the configuration of chitosan schiff-base original solution: the chitosan schiff-base sample dissolves with 0.1mol/L HCl solution, and original concentration is 10mg/mL.
3. the amount of chitosan-containing schiff bases bactericide in the medium: respectively 4mL, 2ml and 200 μ l chitosan schiff-base original solutions are added in the medium of 60 ℃ of 36ml, 38ml and 39.8ml, (wherein 60 ℃ medium is the culture medium after sterilization heat fused, is positioned over then and is cooled to 60 ℃ in the water-bath) is made into the medium that content of dispersion is respectively 1000ppm, 500ppm and 50ppm.
4. adopt the bacteriostatic experiment of growth rate method, the steps include:
(1) with the positive contrast of 50% carbendazol wettable powder (Weihai Korea Spro inspires confidence in biochemical pesticide Co., Ltd), with the negative contrast of 0.1mol/L HCl solution;
(2) medium that will contain chitosan-containing schiff bases bactericide is evenly poured in the culture dish that diameter is 9cm, and do one group parallel;
(3) treat that medium solidifies fully after, in each culture dish the inoculation 3 bacterium cakes that diameter is 6mm;
(4) cultivate: cultivate 48h or 72h down at 25 ℃;
(5) measure colony diameter, each bacterium colony is measured maximum gauge and minimum diameter;
(6) bacteriostasis rate of calculation sample;
(7) repetitive operation bacteriostatic experiment
5. experimental result
A-L represents Monilinia fructicola, fruit white rot of grape bacterium, Valsa mali, tomato early blight bacterium, tomato late blight, cotton-wilt fusarium, wheat rust bacterium, Sclerotinia sclerotiorum, Rhizoctonia solani Kuhn, pear cucumerinum, bacterium of downy mildew of cucumber, corn smut bacterium respectively.I-VII represents second imino group shitosan, the third imino group shitosan, fourth imino group shitosan, benzene auxotox radical shitosan, 2-hydroxy benzenes auxotox radical shitosan, 2-hydroxyl-5-chlorobenzene auxotox radical shitosan, 2-hydroxyl-5-nitrobenzoyl imino group shitosan respectively.
Embodiment 2
The configuration of the purchasing of medium, chitosan schiff-base original solution and bacteriostatic experiment are with example 1, and difference is:
1. the amount of chitosan-containing schiff bases bactericide in the medium: respectively 6ml and 20 μ l chitosan schiff-base original solutions are added in the medium of 34ml and 40ml60 ℃, be made into the medium that content of dispersion is respectively 5ppm, 1500ppm.
2. experimental result
Claims (4)
1. agricultural bacteriocide is characterized in that: adopt chitosan schiff-base, its concentration is: 5ppm-1500ppm.
2. by the described chitosan schiff-base bactericide of claim 1, it is characterized in that: concentration is: 50ppm-1000ppm.
3. by the described chitosan schiff-base bactericide of claim 1, it is characterized in that: used chitosan schiff-base is second imino group shitosan, the third imino group shitosan, fourth imino group shitosan, benzene auxotox radical shitosan, 2-hydroxy benzenes auxotox radical shitosan, 2-hydroxyl-5-chlorobenzene auxotox radical shitosan or 2-hydroxyl-5-nitrobenzoyl imino group shitosan.
4. application by the described agricultural bacteriocide of claim 1, it is characterized in that: described agricultural bacteriocide can suppress 12 kinds of common agricultural diseases; Described 12 kinds of germs are Monilinia fructicola, fruit white rot of grape bacterium, Valsa mali, tomato early blight bacterium, tomato late blight, cotton-wilt fusarium, wheat rust bacterium, Sclerotinia sclerotiorum, Rhizoctonia solani Kuhn, pear cucumerinum, bacterium of downy mildew of cucumber or corn smut bacterium.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100474460A CN1312991C (en) | 2005-10-19 | 2005-10-19 | Agricultural bactericidal agent |
| PCT/CN2006/001789 WO2007045139A1 (en) | 2005-10-19 | 2006-07-21 | Agricultural bactericides and the use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100474460A CN1312991C (en) | 2005-10-19 | 2005-10-19 | Agricultural bactericidal agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1745629A CN1745629A (en) | 2006-03-15 |
| CN1312991C true CN1312991C (en) | 2007-05-02 |
Family
ID=36165478
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100474460A Expired - Fee Related CN1312991C (en) | 2005-10-19 | 2005-10-19 | Agricultural bactericidal agent |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN1312991C (en) |
| WO (1) | WO2007045139A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105211108A (en) * | 2015-10-27 | 2016-01-06 | 苏州科大微龙农业科技有限公司 | A kind of control cutworm insect pest combination control agent |
| CN105660641A (en) * | 2016-03-03 | 2016-06-15 | 苏杨 | Resistance inducing drug for preventing and treating mulberry sorosis disease |
| CN105638664A (en) * | 2016-03-03 | 2016-06-08 | 苏杨 | Method for controlling sclerote disease of mulberry fruit |
| WO2018042311A1 (en) * | 2016-08-29 | 2018-03-08 | Swasti Agro & Bioproducts Pvt Ltd. | Chitosan derivative formulations for plant growth, and building disease resistance |
| CN108076927A (en) * | 2017-12-23 | 2018-05-29 | 全椒县潘林园林葡萄专业合作社 | A kind of control method of fruit white rot of grape |
| CN109627357B (en) * | 2018-12-21 | 2021-03-26 | 中国科学院烟台海岸带研究所 | bis-Schiff base chitosan derivative and preparation method and application thereof |
| CN112715556A (en) * | 2020-12-24 | 2021-04-30 | 云南省烟草公司曲靖市公司 | Medicament for preventing and controlling concentrated attack area of tobacco black shank and use method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6420087A (en) * | 1987-07-13 | 1989-01-24 | Kanai Gakuen | Immobilization of enzyme or bacterium cell using chitosan-aldehyde gel as carrier |
| JPH04173091A (en) * | 1990-11-05 | 1992-06-19 | Showa Denko Kk | Production of n-methylenechitosan gel and method for immobilizing enzyme |
| JPH05163164A (en) * | 1991-12-17 | 1993-06-29 | Daicel Chem Ind Ltd | Separation agent |
-
2005
- 2005-10-19 CN CNB2005100474460A patent/CN1312991C/en not_active Expired - Fee Related
-
2006
- 2006-07-21 WO PCT/CN2006/001789 patent/WO2007045139A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6420087A (en) * | 1987-07-13 | 1989-01-24 | Kanai Gakuen | Immobilization of enzyme or bacterium cell using chitosan-aldehyde gel as carrier |
| JPH04173091A (en) * | 1990-11-05 | 1992-06-19 | Showa Denko Kk | Production of n-methylenechitosan gel and method for immobilizing enzyme |
| JPH05163164A (en) * | 1991-12-17 | 1993-06-29 | Daicel Chem Ind Ltd | Separation agent |
Non-Patent Citations (2)
| Title |
|---|
| 南京化工大学学报(自然科学版) 张所信 江龙法 王为国 耿波，第89-91页，球形壳聚糖希夫碱的制备及性质 作者】 1999 * |
| 西北大学学报(自然科学版) 高朋召 胡道道 唐宗薰 房喻，第115-118，壳聚糖希夫碱钴配合物的合成及表征 2001 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1745629A (en) | 2006-03-15 |
| WO2007045139A1 (en) | 2007-04-26 |
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Granted publication date: 20070502 Termination date: 20131019 |