CN105660641A - Resistance inducing drug for preventing and treating mulberry sorosis disease - Google Patents
Resistance inducing drug for preventing and treating mulberry sorosis disease Download PDFInfo
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- CN105660641A CN105660641A CN201610117516.3A CN201610117516A CN105660641A CN 105660641 A CN105660641 A CN 105660641A CN 201610117516 A CN201610117516 A CN 201610117516A CN 105660641 A CN105660641 A CN 105660641A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a resistance inducing drug for preventing and treating mulberry sorosis disease, prepared from chitosan. The resistance inducing drug for preventing and treating the mulberry sorosis disease has a better prevention and control effect on the mulberry sorosis disease in the early period by taking the chitosan as a mulberry sorosis disease resistance inducer, can effectively replace high-toxicity high-persistent pesticides to be used, can reduce mulberry pest and disease damage prevention and control operation risks in a production process to avoid human and animal poisoning accidents, can remarkably reduce non-point source pollution caused by the pesticides and waste thereof, and contributes to protecting an agroecological environment.
Description
Technical field
The invention belongs to the technical field of the biological prevention and control of diseases and pests of agronomic crop, specifically relate to that a kind of to prevent and treat luring of diseases of mulberry fruits anti-Medicament.
Background technology
Diseases and pests of agronomic crop green prevention and control, refers to environment friends such as taking ecological regulation and control, biological control, physical control and Scientific Usage of DrugsThe vegetative practice of good type measure control crop diseases and pest harm; To belong to resource-conserving and environmentally friendly technology. It isThe extension of Study on Comprehensive Pest Control, although integrated control and green prevention and control are all the Pest managements of requirement risk of selection minimumMaterial, but green prevention and control emphasize be use derive from plant or be directed to natural organic material.
At present, the crop pest control of China including mulberry tree, all too relies on Chemical control methods. This measure is pastToward in controlling sick worm harm loss, often bring sick worm resistance to the action of a drug rising and sick worm to break out the negative issues such as probability increase.On the other hand, traditional crop pest control measure had not both met the demand for development of modern agriculture, can not meet agricultural markThe needs that standardization is produced.
Therefore, large-scale promotion diseases and pests of agronomic crop green prevention and control technology, can effectively solve in crops standardized production processA prevention and control of plant diseases, pest control difficult problem, significantly reduce the use amount of chemical pesticide, avoid the residues of pesticides in agricultural product to exceed standard, promote agricultural productionProduct quality level of security, increases the market competitiveness, promotes peasant's increasing both production and income. Meanwhile, by applying green prevention and control technology,Contribute to protect bio-diversity, reduce disease and pest and break out probability, realize the sustainable control of disease and pest, be also conducive to alleviate diseaseWorm harm loss, ensures the effective supply of grain good harvest and main farm produce. In addition, by effectively promoting biological control, thingReason control waits green prevention and control technology, can not only effectively substitute the use of high poison, persistent pesticide, can also reduce in production processDisease and pest prevention and control operating risk, avoids person poultry poisoning's accident. Meanwhile, also significantly reduce the face source dirt that agricultural chemicals and discarded object thereof causeDye, contribute to protect agroecological environment.
Plant disease lure anti-medicament present situation and market situation as follows:
(1) oligomerization glucose (Oligoglucan), oligogalacturonans (Oligochitosan) and chitin oligosaccharidesEtc. (Oligochitin) can improve the disease resistance of Genes For Plant Tolerance to fungi etc.
(2) probenazole (Probenazole), it is the important bactericide kind of Japan, itself is withered and leaf to rice blast fungus, lineRot bacterium does not have activity, but can strengthen the resistance of paddy rice to disease.
(3) Benzothiadiazole (Benzothiadiazole, BTH), itself does not have bactericidal activity, can improve the anti-of many cropsCharacteristic of disease.
(4) jasmonic (JA) and methyl jasmonate (MeJA), as exogenous application, can excite the table of defence plant geneReach the chemical defence of inducing plant, the effect that generation is similar with insect's food-taking to mechanical damage
(5) Brassinosteroids (Brassinosteroid, BR) and brassinosteroid (Brassinolide, BL) can be carriedThe ability that high plant disease-resistant and cold-resistant etc. is coerced. External source BR can induce and plant in suitable concn and specific growth and development of plants stageDeposits yields disease resistance response, BL can improve the resistance of tobacco to multiple pathogens such as fungi, bacterium, viruses
Present stage market analysis: conventional agriculture chemicals fungicide in the market, by the mode of action can be divided into protective agent, therapeutic agent andEradicant, before protective agent refers to that pathogenic microorganism does not contact plant or do not immerse plant, with medicament is processed plant or aroundEnvironment, reaches the Spores that suppresses Spores sprouting or kill sprouting, avoids its evil with protective plant, mainly contains BordeauxLiquid, zineb, copper sulphate, green newborn copper, Mancozeb, Bravo etc.; Therapeutic agent refers to that pathogenic microorganism has immersed plantIn body, but plant performance illness is in incubation period, and medicine oozes people's plant tissue inside from plant epidermis, through transporting, diffusion orProduce metabolin and kill or suppress cause of disease, diseased plant is no longer injured, and gets well, as thiophanate methyl, carbendazim, springThunder mycin etc. Eradicant refers to that the susceptible rear dispenser of plant can directly kill the pathogen of invaded plants. There is this eradicant actionAgriculture chemicals fungicide is eradicant. As asomate, penta sodium pentachlorophenate, lime sulfur etc.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of anti-medicament of luring of diseases of mulberry fruits of preventing and treating, this control diseases of mulberry fruitsLure anti-medicament by chitosan oligosaccharide as diseases of mulberry fruits inducer, early period of origination diseases of mulberry fruits is had to better prevention and control effect, thisThe use of high poison, persistent pesticide can not only be effectively substituted, the Mulberry Diseases And Pests prevention and control operating risk in production process can also be reduced,Avoid person poultry poisoning's accident, also significantly reduce the pollution of area source that agricultural chemicals and discarded object thereof cause simultaneously, contribute to protect Agro-ecologyEnvironment.
In order to solve above-mentioned technical problem, a kind of anti-medicament of luring of diseases of mulberry fruits of preventing and treating of the present invention, comprises shitosan.
Further, the concentration of described shitosan is 2.5mg/L～5.0mg/L.
The invention has the beneficial effects as follows:
During diseases of mulberry fruits of the present invention breaks out, the green exploitation that lures anti-medicament, both can fill up diseases of mulberry fruits green and lure anti-medicamentBlank, can provide a kind of method and reference for its crop bacterial disease green prevention and control again; Both can meet silkworm industry and produce upper mulberry fruitSclerotiniose actual needs, for this industry good harvest provides safeguard, can meet again mankind's environmental protection, the healthy demand that continues, greenWhat look prevention and control were emphasized is to use to derive from plant or be directed to natural organic material. We adopt chitosan oligosaccharide as mulberry fruitSclerotiniose inducer, has better prevention and control effect to early period of origination diseases of mulberry fruits, and this can not only effectively substitute high poison, high residue agricultureThe use of medicine, can also reduce the Mulberry Diseases And Pests prevention and control operating risk in production process, avoids person poultry poisoning's accident. Also meanwhile,Significantly reduce the pollution of area source that agricultural chemicals and discarded object thereof cause, contribute to protect agroecological environment, field application concentration is 2.5Mg/L～5.0mg/L, has and lures preferably anti-effect sick mulberry tree.
Detailed description of the invention
A kind of anti-medicament of luring of diseases of mulberry fruits of preventing and treating of the present invention, comprises shitosan.
Further, the concentration of described shitosan is 2.5mg/L～5.0mg/L.
(1) field test mulberry field: select physical features, soil fertility, cultivation, 0.5 hectare of the consistent generation area of management factorsNatural occurrence mulberry field, there is mulberry field in diseases of mulberry fruits former years; Test mulberry tree breed is to diseases of mulberry fruits susceptible variety: large 10,In production, large area is used training improved seeds.
(2) community (sample prescription) design: in same mulberry field, arrange Zu Mei community, the district (sample of random alignment by random district groupSide) there are 6 row mulberry treies, every row selects 3～4 strain mulberry treies, totally 20 strain mulberry treies, every community surrounding has zone of protection more than 3m.Choose altogether 21Ge community (sample prescription).
(3) test medicine application design: test medicine chitosan oligosaccharide liquid by 6 concentration gradients (20.0,16.0,13.0,10.0,5.0、2.5mg·L-1) process, separately establishing clear water is control treatment. 7 are processed each repetition 3 times (is all that interval connects for one week at every turnContinuous medication 5 times), process repetition for totally 21.
(4) field medication: 21 processing are repeated to use conventional spray-on process, and random even spraying is to choosing 21Ge communityOn (sample prescription) inner mulberry leaf, single treatment repeats the sample prescription of only spraying independently,
(5) field investigation data are recorded: investigation period: after dispenser 25 days and carry out the investigation of state of an illness radix 40 day morning,With morbidity strain and morbidity branch. Defining of sick branch: with observation unit, as long as a branch has a sick fruit, be all judged to be this branchMorbidity; Defining of diseased plant: on every strain mulberry tree, as long as there is one to be disease branch, be just judged to be this tree for morbidity strain; Branch morbidityClassification is defined: 0 grade=all mulberry fruits are for normal, 1 grade=to have a small amount of 1 mulberry fruit be gingko, 2 grades=1/3 sick fruit, 3 grades=1/2Sick fruit more than 4 grades=1/2 is disease fruit.
(6) induction of resistance effect is calculated:
Induction of resistance potential with strain incidence of disease quantification chitosan oligosaccharide to whole strain mulberry tree, the strain incidence of disease is less, shows induction of resistance potentialGood, be calculated as follows the strain incidence of disease:
The total strain number of the strain incidence of disease=morbidity strain number/investigate
Weigh chitosan oligosaccharide to diseases of mulberry fruits induction of resistance trend with the branch incidence of disease, the branch incidence of disease is less, shows that induction of resistance becomesGesture is more obvious, is calculated as follows an incidence of disease:
The total branch number of a branch incidence of disease=morbidity number/investigate
By relative prevention effect as chitosan oligosaccharide to diseases of mulberry fruits induction of resistance overall objective, its numerical value is larger, and inducer is describedIt is better that mulberry tree induction of resistance is produced
Prevention effect (%) relatively=(contrast disease index-processing disease index)/contrast disease index × 100
Wherein, disease index (Y) computing formula is as follows:
Y=∑ (the sick fruit of rank typical value × this rank number)/(the total fruit of investigation number × highest level value) × 100
(7) other details: by People's Republic of China's agricultural industry criteria " mulberry field practical technique code " (NY/T1027-2006) biological detection (normative appendix) of the mulberry medicine drug effect in the mensuration of the B.1 field control effect in Appendix B is enteredOK.
Embodiment 1
The present embodiment illustrates with this product diseases of mulberry fruits induction of resistance potential.
Chitosan oligosaccharide 20.0,16.0,13.0,10.0,5.0 and 2.5mgL-1Totally 6 concentration processing, establishing clear water is contrast placeReason. Process each repetition 3 times for 7, process repetition for totally 21. Select gentle breeze to start medication the morning on May 1st, 2015 without rain.
Induction of resistance potential with strain incidence of disease quantification chitosan oligosaccharide to whole strain mulberry tree, the strain incidence of disease is less, shows induction of resistance potentialGood, concentration is 2.5mgL-1After 25 days, the average preventive effect of strain is after 72.22%, 40 day to be 34.29%.
Embodiment 2
The present embodiment is used for illustrating that chitosan oligosaccharide is to diseases of mulberry fruits induction of resistance trend.
Chitosan oligosaccharide 20.0,16.0,13.0,10.0,5.0 and 2.5mgL-1Totally 6 concentration processing, establishing clear water is contrast placeReason. Process each repetition 3 times for 7, process repetition for totally 21. Select gentle breeze to start medication the morning on May 3rd, 2015 without rain.
Weigh chitosan oligosaccharide to diseases of mulberry fruits induction of resistance trend with the branch incidence of disease, the branch incidence of disease is less, shows that induction of resistance becomesGesture is more obvious, 5.0mgL-1After 25 days, prevention effect reaches 9.13%, 2.5mgL-1After 40 days, prevention effect reaches 77.36%,Illustrate under low concentration, induction effect also shows better.
Embodiment 3
The present embodiment is to diseases of mulberry fruits induction of resistance population effect for explanation.
Press chitosan oligosaccharide 20.0,16.0,13.0,10.0,5.0 and 2.5mgL-1Totally 6 concentration processing, establish clear water for contrastProcess. Process each repetition 3 times for 7, process repetition for totally 21. Select gentle breeze to start the morning on May 5th, 2015 to use without rainMedicine.
By relative prevention effect as chitosan oligosaccharide to diseases of mulberry fruits induction of resistance overall objective, its numerical value is larger, and inducer is describedIt is better that mulberry tree induction of resistance is produced. 5.0mgL-1After 40 days, prevention effect reaches 62.11%, 2.5mgL-1Control afterwards in 40 daysEffect reaches 75.22%.
Table one, the induction of resistance ability comparision (%) of chitosan oligosaccharide to whole strain mulberry tree
Table two, chitosan oligosaccharide are to diseases of mulberry fruits induction of resistance trend
Table three, chitosan oligosaccharide are totally induced effect (%) to diseases of mulberry fruits induction of resistance
Finally explanation, above preferred embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by upperState preferred embodiment and the present invention is described in detail, but it will be appreciated by those skilled in the art that can be in formWith in details, it is made to various changes, and do not depart from the claims in the present invention book limited range.
Claims (2)
1. prevent and treat the anti-medicament of luring of diseases of mulberry fruits, it is characterized in that: comprise shitosan.
Control diseases of mulberry fruits as claimed in claim 1 lure anti-medicament, it is characterized in that: the concentration of described shitosan is 2.5mg/L～5.0mg/L。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610117516.3A CN105660641A (en) | 2016-03-03 | 2016-03-03 | Resistance inducing drug for preventing and treating mulberry sorosis disease |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610117516.3A CN105660641A (en) | 2016-03-03 | 2016-03-03 | Resistance inducing drug for preventing and treating mulberry sorosis disease |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989001288A1 (en) * | 1987-08-14 | 1989-02-23 | Washington State University Research Foundation, I | Method for treating cereal crop seed with chitosan to enhance yield, root growth, and stem strength |
| CN1745629A (en) * | 2005-10-19 | 2006-03-15 | 中国科学院海洋研究所 | A kind of agricultural bacteriocide |
| CN101095459A (en) * | 2007-06-23 | 2008-01-02 | 山东省海水养殖研究所 | Method for compressing marine microalgae |
| CN103449590A (en) * | 2013-09-11 | 2013-12-18 | 中国矿业大学 | Method for safely removing algae and settling algae based on bentonite |
-
2016
- 2016-03-03 CN CN201610117516.3A patent/CN105660641A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989001288A1 (en) * | 1987-08-14 | 1989-02-23 | Washington State University Research Foundation, I | Method for treating cereal crop seed with chitosan to enhance yield, root growth, and stem strength |
| CN1745629A (en) * | 2005-10-19 | 2006-03-15 | 中国科学院海洋研究所 | A kind of agricultural bacteriocide |
| CN101095459A (en) * | 2007-06-23 | 2008-01-02 | 山东省海水养殖研究所 | Method for compressing marine microalgae |
| CN103449590A (en) * | 2013-09-11 | 2013-12-18 | 中国矿业大学 | Method for safely removing algae and settling algae based on bentonite |
Non-Patent Citations (1)
| Title |
|---|
| 吕道坤 等: "桑椹白果病综合防治效果研究", 《现代农业科技》 * |
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Application publication date: 20160615 |