CN1310953A - Honey mushroom noodles and industrialized liquid fermentation producing technology for honey mushroom bacterial strain - Google Patents
Honey mushroom noodles and industrialized liquid fermentation producing technology for honey mushroom bacterial strain Download PDFInfo
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- CN1310953A CN1310953A CN00135443A CN00135443A CN1310953A CN 1310953 A CN1310953 A CN 1310953A CN 00135443 A CN00135443 A CN 00135443A CN 00135443 A CN00135443 A CN 00135443A CN 1310953 A CN1310953 A CN 1310953A
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Abstract
The present invention relates to shoestring fungus noodle and shoestring fungus strain liquid fermentation production technology. It is characterized in that: (1). 30T shoestring fungus strain fermentation liquid is added to the noodle formulation; (2). the shoestring fungus strain fermentation technology includes four steps: fungus species selection, slant culture, liquid species culture and liquid fermentation production, in which the liquid fermentation production adopts 1st, 2nd species tank and 3rd fermentation tank production. The shoestring fungus possesses the pharmacological actions of calm, anti convulsion, enhance immune function etc. Advantages: short production period, high yield, stable quality etc.
Description
The present invention relates to a kind of edible mushroom noodles, exactly is honey mushroom noodles and industrialized liquid fermentation producing technology for honey mushroom bacterial strain.
Halimasch has another name called the hazel mushroom, is the fungal component of rhizoma Gastrodiae.It is nutritious, contains 18 seed amino acids, and total amount is 11.37 grams, and wherein the highest to the useful glutamic acid of cranial nerve, aspartic acid content, methionine content is minimum.Macroelement calcium, potassium, magnesium, phosphorus content necessary in the human body are the highest, also contain 6 kinds of trace elements irons, chromium, copper, manganese, boron, the zinc of needed by human body simultaneously; Be rare food, medicine dual-purpose bacterium.
At present, Duan Mu and substituting stuff cultivation are mainly adopted in the production of halimasch, and the production cycle is long, and be segment wood cultivated very big to the influence of forest accumulation amount, and unstable product quality.
The purpose of this utility model is: provide a kind of nutritious, honey mushroom noodles with food therapy effect and with short production cycle, output is high, steady quality, industrialized liquid fermentation producing technology for honey mushroom bacterial strain that production efficiency is high.
Concrete technical solution is such.
Honey mushroom noodles, raw materials used is flour, it is characterized in that: the halimasch RCEF769 bacterial strain fermentation liquor with 30% adds in the flour.
Halimasch RCEF769 industrial liquid fermentation producing technology for bacterial strain is characterized in that:
1, strain separating
The halimasch bacterial strain separates the shoestring of arrogant other mountain area rhizoma Gastrodiae;
2, slant strains is cultivated
The bacterial classification inoculation of separation and purification in the PDA medium slant, is put into incubator, and 25 ℃ of cultivations treat that mycelia covers with the inclined-plane;
3, liquid seeds is cultivated
With culture medium pack into 250ml or 500ml triangular flask, every bottled amount is respectively 100ml or 200ml, 1.5Pa/Kg autoclaving 30min, culture medium after sterilization liquid is to be cooled after 35～45 ℃, inserts slant strains, inoculum concentration is that 250ml props up, 500ml connects 2, and inoculation is placed on the constant-temperature shaking culture case, at 25 ℃, under the 120rpm condition, cultivate 6;
Described liquid seed culture medium is: 5% wheat bran liquor, 2% is from granulated sugar, 0.5% dried silkworm chrysalis meal, 0.15%KH
2PO
4, 0.075%MgSO
4, Cobastab
10.001% and 92.274% water, pH6.0～7.0.
4, three grade fermemtation is adopted in liquid fermentation production,
(1), first class seed pot production
The 50L liquid medium of packing in the 70L airlift fermentor, and add 0.03% defoamer polyoxypropylene ethylene oxide glycerin ether, 14.7 * 10
4Under the pa pressure, pressurize 30min inserts cultivation, inoculum concentration 10%, 24～26 ℃ of cultivation temperature, throughput 1: 0.5,4.903～7.0845 * 10 with shake-flask seed after the sterilization cooling
4Pressurize 36～48h ventilation is cultivated under the Pa pressure condition, when the mycelia raised growth reaches exponential phase, can insert the secondary seed jar;
(2), the secondary seed jar is produced
The 500L liquid medium of packing in the 700L airlift fermentor, other controlled condition is the same, incubation time 48～60h;
(3), the three grade fermemtation jar is produced
The 5T liquid medium of packing in the 7T airlift fermentor; Temperature is higher than 95 ℃, and feeds Steam Heating to 121 ℃, behind the pressurize 15min, is cooled to 24～26 ℃, and cultured secondary seed solution is inserted fermentation tank, and ventilation is cultivated, throughput 1: 0.4～0.5, tank pressure 4.9033 * 10
4Pa, incubation time 72～96h;
When the zymotic fluid residual sugar below 1%, zymotic fluid is than thickness; Notice whether mycelia attenuates during microscopy in addition; Have or not tangible vacuole in the mycelium; And have or not obvious fracture in the zymotic fluid, and if said circumstances has one to several appearance, can go out jar, get halimasch RCEF769 zymotic fluid.
Described I and II seeding tank, three grade fermemtation jar Liquid Culture based formulas are: 1% white granulated sugar, 2% soluble starch, 3% dusty yeast, 0.15%KH
2PO
4, 0.1%MgSO
4With 93.75% water, pH6.0～7.0.
The present invention adopts industrial fermentation technology, and not only protect the forest reserves, shortened the production cycle, and output height, steady quality, productivity effect height.The halimasch liquid fermented and cultured can be produced continuously, is not subjected to restrictions such as production site, season.Simultaneously, the bioactivator in the zymotic fluid as: polysaccharide, polypeptide, alkaloid, terpenoid etc. all can further be utilized.
Honey mushroom noodles of the present invention has not only kept the deliciousness of halimasch, and is rich in the various nutritional labelings of halimasch, and medicine-food two-purpose can improve body immunity, and has sedation, can reach food therapy effect.
Below by embodiment the present invention is done to describe further.
Embodiment:
Honey mushroom noodles, the raw materials used flour that comprises.Halimasch RCEF769 bacterial strain fermentation liquor with 30% adds in the flour, is pressed into noodles behind the placement 30min, packing aluminium pool nitrogen-filled packaging after the low temperature drying.
Industrialized liquid fermentation producing technology for honey mushroom bacterial strain, being divided into is four big operations:
1, strain separating
The halimasch bacterial strain separates the shoestring of arrogant other mountain area rhizoma Gastrodiae.
2, slant strains is cultivated
The bacterial classification inoculation of separation and purification in the PDA medium slant, is put into incubator, and 25 ℃ of cultivations treat that mycelia covers with the inclined-plane; Can change over to and shake the bottle cultivation.
3, liquid seeds is cultivated
With culture medium pack into 250ml or 500ml triangular flask, every bottled amount is respectively 100ml or 200ml, 1.5Pa/Kg autoclaving 30min, culture medium after sterilization liquid is to be cooled after 35～45 ℃, inserts slant strains, inoculum concentration is that 250ml props up, 500ml connects 2, and inoculation is placed on the constant-temperature shaking culture case, at 25 ℃, under the 120rpm condition, cultivate 6d and get final product.
Described liquid seed culture medium is: 5% wheat bran liquor, 2% white granulated sugar, 0.5% dried silkworm chrysalis meal, 0.15%KH
2PO
4, 0.075%MgSO
4, Cobastab
10.001% and 92.274% water, pH6.0～7.0.
4, three grade fermemtation is adopted in liquid fermentation production, is example with 70L, 700L, 7T fermentation tank.
(1), first class seed pot production
The 50L liquid medium of packing in the 70L airlift fermentor, water temperature is higher than 95 ℃, and adds 0.03% defoamer polyoxypropylene ethylene oxide glycerin ether, 14.7 * 10
4Under the pa pressure, pressurize 30min inserts cultivation with shake-flask seed after the sterilization cooling, inoculum concentration 10%, and 24～26 ℃ of cultivation temperature, throughput 1: 0.5 is 4.903～7.0845 * 10
4Under the Pa pressure condition, pressurize 36～48h cultivation of ventilating when the mycelia raised growth reaches exponential phase, can insert the secondary seed jar.
(2), the secondary seed jar is produced
The 500L liquid medium of packing in the 700L airlift fermentor, other controlled condition is the same, incubation time 48～60 hours.
(3), the three grade fermemtation jar is produced
The 5T liquid medium of packing in the 7T airlift fermentor, coolant-temperature gage is higher than 95 ℃, and feed Steam Heating to 121 ℃, attention: liquid amount is controlled at 80% of fermentation tank volume, is cooled to 24～26 ℃ behind the pressurize 15min, and cultured secondary seed solution is inserted fermentation tank, inoculum concentration 10%, ventilation is cultivated, throughput 1: 0.4～0.5, tank pressure 4.9033 * 10
4Pa, incubation time 72～96h.
When the zymotic fluid residual sugar below 1%, zymotic fluid is than thickness, notices whether mycelia attenuates during microscopy in addition, has or not tangible vacuole in the mycelium, and have or not obvious fracture in the zymotic fluid, if said circumstances has one to several appearance, can go out jar, get halimasch RCEF769 liquid fermentation liquid.
I and II seeding tank, three grade fermemtation jar Liquid Culture based formulas are: 1% white granulated sugar, 2% soluble starch, 3% dusty yeast, 0.15%KH
2PO
4, 0.1%MgSO
4With 93.75% water, pH6.0～7.0.
Claims (4)
1, honey mushroom noodles, raw materials used is flour, it is characterized in that: the halimasch RCEF769 bacterial strain fermentation liquor with 30% adds in the flour.
2, halimasch RCEF769 industrial liquid fermentation producing technology for bacterial strain is characterized in that:
2.1, strain separating
The halimasch bacterial strain separates the shoestring of arrogant other mountain area rhizoma Gastrodiae;
2.2, slant strains cultivates
The bacterial classification inoculation of separation and purification in the PDA medium slant, is put into incubator, and 25 ℃ of cultivations treat that mycelia covers with the inclined-plane;
2.3, liquid seeds cultivates
With culture medium pack into 250ml or 500ml triangular flask, every bottled amount is respectively 100ml or 200ml, 1.5Pa/Kg autoclaving 30min, culture medium after sterilization liquid is to be cooled after 35～45 ℃, inserts slant strains, inoculum concentration is that 250ml props up, 500ml connects 2, and inoculation is placed on the constant-temperature shaking culture case, at 25 ℃, under the 120rpm condition, cultivate 6d;
2.4, liquid fermentation production, adopt three grade fermemtation,
(1), first class seed pot production
The 50L liquid medium of packing in the 70L airlift fermentor, and add 0.03% defoamer polyoxypropylene ethylene oxide glycerin ether, 14.7 * 10
4Under the Pa pressure, pressurize 30min inserts cultivation, inoculum concentration 10%, 24～26 ℃ of cultivation temperature, throughput 1: 0.5,4.903～7.0845 * 10 with shake-flask seed after the sterilization cooling
4Pressurize 36～48h ventilation is cultivated under the Pa pressure condition, when the mycelia raised growth reaches exponential phase, can insert the secondary seed jar;
(2), the secondary seed jar is produced
The 500L liquid medium of packing in the 700L airlift fermentor, other controlled condition is the same, incubation time 48～60h;
(3), the three grade fermemtation jar is produced
The 5T liquid medium of packing in the 7T airlift fermentor; Temperature is higher than 95 ℃, and feeds Steam Heating to 121 ℃, behind the pressurize 15min, is cooled to 24～26 ℃, and cultured secondary seed solution is inserted fermentation tank, inoculum concentration 10%, and ventilation is cultivated, throughput 1: 0.4～0.5, tank pressure 4.9033 * 10
4Pa, incubation time 72～96h;
When the zymotic fluid residual sugar below 1%, zymotic fluid is than thickness, notices whether mycelia attenuates during microscopy in addition, has or not tangible vacuole in the mycelium, and have or not obvious fracture in the zymotic fluid, if said circumstances has one to several appearance, can go out jar, get halimasch RCEF769 zymotic fluid.
3, halimasch RCEF760 industrial liquid fermentation producing technology for bacterial strain according to claim 2, it is characterized in that: described liquid seed culture medium is: 5% wheat bran liquor, 2% white granulated sugar, 0.5% dried silkworm chrysalis meal, 0.15%KH
2PO
4, 0.075%MgSO
4, Cobastab
10.001% and 92.274% water, pH6.0～7.0.
4, halimasch RCEF769 industrial liquid fermentation producing technology for bacterial strain according to claim 2 is characterized in that: described I and II seeding tank, three grade fermemtation jar Liquid Culture based formulas are: 1% white granulated sugar, 2% soluble starch, 3% dusty yeast, 0.15%KH
2PO
4, 0.1%MgSO
4With 93.75% water, pH6.0～7.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00135443A CN1109509C (en) | 2000-12-21 | 2000-12-21 | Honey mushroom noodles and industrialized liquid fermentation producing technology for honey mushroom bacterial strain |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00135443A CN1109509C (en) | 2000-12-21 | 2000-12-21 | Honey mushroom noodles and industrialized liquid fermentation producing technology for honey mushroom bacterial strain |
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| Publication Number | Publication Date |
|---|---|
| CN1310953A true CN1310953A (en) | 2001-09-05 |
| CN1109509C CN1109509C (en) | 2003-05-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00135443A Expired - Fee Related CN1109509C (en) | 2000-12-21 | 2000-12-21 | Honey mushroom noodles and industrialized liquid fermentation producing technology for honey mushroom bacterial strain |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103751271A (en) * | 2014-02-20 | 2014-04-30 | 通化吉通药业有限公司 | Qiangli Naoxinkang (one of medicine names) tablets and preparation method thereof |
| CN103798055A (en) * | 2013-05-26 | 2014-05-21 | 程立君 | Method for producing rhizoma gastrodiae companion armillaria mellea through liquid submerged fermentation |
| CN107213172A (en) * | 2017-06-13 | 2017-09-29 | 颜鑫 | Asclerol oral liquid and preparation method thereof |
| CN115992055A (en) * | 2022-11-23 | 2023-04-21 | 湖北诺克特药业股份有限公司 | Process method for circularly dividing and supplementing materials to ferment armillaria mellea |
-
2000
- 2000-12-21 CN CN00135443A patent/CN1109509C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103798055A (en) * | 2013-05-26 | 2014-05-21 | 程立君 | Method for producing rhizoma gastrodiae companion armillaria mellea through liquid submerged fermentation |
| CN103751271A (en) * | 2014-02-20 | 2014-04-30 | 通化吉通药业有限公司 | Qiangli Naoxinkang (one of medicine names) tablets and preparation method thereof |
| CN107213172A (en) * | 2017-06-13 | 2017-09-29 | 颜鑫 | Asclerol oral liquid and preparation method thereof |
| CN115992055A (en) * | 2022-11-23 | 2023-04-21 | 湖北诺克特药业股份有限公司 | Process method for circularly dividing and supplementing materials to ferment armillaria mellea |
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| Publication number | Publication date |
|---|---|
| CN1109509C (en) | 2003-05-28 |
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Owner name: HEFEI MAIKELUO BIOLOGICAL ENGINEERING CO.,LTD. Free format text: FORMER OWNER: ANHUI LINGYUAN AWETO RESEARCH INSTITUTE Effective date: 20080530 |
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Effective date of registration: 20080530 Address after: Room 128, building 106, Anhui Agriculture University, Anhui, Hefei Patentee after: Hefei Micro Biological Engineering Co., Ltd. Address before: 753 mailbox, Anhui Agriculture University, 130 West Changjiang Road, Hefei, Anhui Patentee before: Anhui Linyuan Chinese Caterpillar Fungus Institute |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20030528 Termination date: 20141221 |
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| EXPY | Termination of patent right or utility model |