CN1306258C - Process for preparing tissue chips and apparatus thereof - Google Patents
Process for preparing tissue chips and apparatus thereof Download PDFInfo
- Publication number
- CN1306258C CN1306258C CNB2004100256457A CN200410025645A CN1306258C CN 1306258 C CN1306258 C CN 1306258C CN B2004100256457 A CNB2004100256457 A CN B2004100256457A CN 200410025645 A CN200410025645 A CN 200410025645A CN 1306258 C CN1306258 C CN 1306258C
- Authority
- CN
- China
- Prior art keywords
- closing member
- sampling probe
- nook closing
- sampling
- organization chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004519 manufacturing process Methods 0.000 title description 6
- 238000005070 sampling Methods 0.000 claims abstract description 81
- 238000002360 preparation method Methods 0.000 claims abstract description 24
- 239000012188 paraffin wax Substances 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000000523 sample Substances 0.000 claims description 81
- 230000008520 organization Effects 0.000 claims description 40
- 239000001993 wax Substances 0.000 claims description 8
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 7
- 239000004575 stone Substances 0.000 claims description 6
- 239000012141 concentrate Substances 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims description 4
- 238000003491 array Methods 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 11
- 230000008569 process Effects 0.000 abstract description 5
- 238000000465 moulding Methods 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract 1
- 238000000018 DNA microarray Methods 0.000 description 4
- 238000009434 installation Methods 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a method and an apparatus for preparing tissue chips. The apparatus is composed of a sampling instrument (I) and a preparation instrument (II) and mainly comprises a control pressure lever (1), a guide sliding block(3), a fixing screw cap (4), sampling needles (5) respectively with a needle core (11), a restoring pressure spring (6), a positioning bolt (7), a guide sliding bar (9), a control screw bolt (12), a needle core control plate (14), an array arrangement plate (15), an L-shaped baffle plate (16) and a guide bar (17). The method comprises: concentratively taking samples by the sampling instrument (I), and then orderly arranging a group of the sampling needles (5) on the array arrangement plate (15); injecting all the tissue samples simultaneously into paraffin at a time by pushing down the needle core control plate (14), and obtaining a tissue chips after the paraffin is solidified. When the apparatus with reasonable structural design in the present invention, acceptor paraffin blocks do not need to be prepared, the process step of perforation is not required, a precise positioning system is not required, single blanks are not required to prepare gradual point samples, and the point samples of all samples and the molding of the paraffin blocks of tissue chips are completed through one step; therefore, the process cost can be lowered, the work efficiency and the quality of the chips can be improved, and the present invention is favorable to the popularization of a chip preparing technology.
Description
Technical field
The invention belongs to biotechnology, relate to tissue array technology, relate in particular to a kind of new preparation method and device thereof of organization chip, be applicable to molecular biology research.
Background technology
Biochip (biochip) is that of growing up the mid-90 in 20th century is used for the high flux of molecular biology research, high-tech technology, be the biotechnology revolution that has significant impact again (the Watson S J after large scale integrated circuit, et al., Biol Psychiatry, 1999).According to the difference of biomaterial, biochip divides multiple again, as genetic chip, protein chip, cell chip, microorganism chip, organization chip etc.1998, (Frantz G D, et al., J Pathol, 2001) such as Kenonen J at first reported tissue array technology, cry again micro-array tissue (tissue microarray, TMA).Use this technology, can carry out the molecular biology research of tissue in situ such as SABC, in situ hybridization simultaneously dozens of and even hundreds and thousands of samples.An important branch as biochip, organization chip has become important tool (the Skacel M that promotes molecular genetics, genomics and proteomics research achievement to transform and study oncogene phenotype and function rapidly to clinical practice, et al., Appl Immunohistochem MolMorphol, 2002) (Torhorst J, et al., Am J Pathol, 2001).
The preparation of organization chip is one of gordian technique of organization chip.At present, the preparation of organization chip mainly to make by hand, need at first prepare blank acceptor wax block, adopts a cover perforating needle and a sampling probe, punch successively respectively, sampling and point sample, and single base repetitive operation, not only work efficiency is low, and is difficult to guarantee chip quality.In addition, general technology is had relatively high expectations to the degree of accuracy of positioning system at present, locatees the whether accurate quality that directly influences chip array, and the relatively more expensive (Liu Lianxin of the organization chip preparation facilities of being furnished with Precise Position System, foreign medical oncology credit volume, 2000,27:83-85).All of these factors taken together has all greatly limited the work efficiency that organization chip is made, and is unfavorable for popularizing and promoting of this technology.The preparation of organization chip becomes one of bottleneck that influences this technology popularization and application.
Existing patent documentation report prepares the method for organization chip, as the Chinese invention patent application number: 02139474.1, put down in writing a kind of method for preparing organization chip, constant water bath box that need be by having temp control switch is in the scope of 5 ℃～10 ℃ of a little higher than its fusing points of temperature of relatively long time inner control melted paraffin, but also the negative pressure pump that need be communicated with the negative pressure box of embedding groove below, form certain negative pressure at the embedding trench bottom, structure is relatively complicated, the technology cost is higher, and remains and repeat point sample one by one.Chinese invention patent application number: 02113230.5, put down in writing the semi-molten state that utilizes paraffin and made organization chip, this method need be kept paraffin semi-molten state for a long time, is not easy to the location in the point sample process, is difficult to manufacture high density, high-quality organization chip.European patent WO 2004/000992 and United States Patent (USP) PAT.NO.:6,103,518, put down in writing the improvement that lays particular emphasis on typical organization's diseased region system of selection, chip manufacturing remains traditional handicraft, and cost of manufacture is higher relatively.
Summary of the invention
An object of the present invention is to provide a kind of new preparation method of organization chip, technical scheme is: after the routine techniques marker samples, finish by the organization chip preparation facilities, at first concentrate sampling by the sampling probe that is fixed in sampling instrument, to have the position preface of one group of sampling probe of sample then according to chip design, be arranged on the array assignment plate successively, then array assignment plate was placed 20 minutes in 4 ℃ of refrigerators, paraffin is filled with in the rectangular channel that is synthesized by two L type baffle group, array assignment plate is put into preparing instrument, turn nook closing member control panel, drive the nook closing member control panel and press down, all tissue samples on the array assignment plate are once injected paraffin simultaneously, treat that paraffin solidifies fully after, take off wax stone, promptly finish the preparation of organization chip.
Another purpose of the present invention provides the organization chip preparation facilities of realizing the inventive method, this device mainly is made up of sampling instrument and preparing instrument, sampling instrument 1 mainly comprises the control depression bar, push rod, guide runner, hold-doun nut, the sampling probe of band nook closing member, recoil press spring, bolt, locating piece, the guiding slide bar, pedestal, the guiding slide bar is set on the pedestal, recoil press spring is connected with guide runner, hold-doun nut is set on the guide runner, sampling probe is fixed by hold-doun nut and guide runner, guide runner is connected with an end of push rod, the other end of push rod is connected with the control depression bar, the control depression bar flexibly connects with the top of guiding slide bar, bolt is connected with the guiding slide bar by locating piece, downslide height with the control guide runner, preparing instrument mainly comprises the control bolt, fixed head, the nook closing member control panel, array assignment plate, L type baffle plate, guide pole, base, guide pole is set on the base, top at guide pole is provided with fixed head, the control bolt passes fixed head and is connected with the nook closing member control panel, the nook closing member control panel is by the movable guide pole that embeds of groove, the nook closing member control panel can be slided up and down along guide pole, and array assignment plate places the rectangular channel top of being made up of L type baffle plate and base.Diameter and the sampling probe point diameter hole of suitable array arrangement mutually is set on the array assignment plate.
Sampling probe and nook closing member activity are nested, sampling probe and nook closing member all have a tail end that expands, make the tail end of sampling probe and nook closing member can not enter the hole that array assignment plate is provided with, the nook closing member front end is inserted in the sampling probe front end, nook closing member tail end slightly larger in diameter is in sampling probe tail end diameter, keep the nook closing member tail end not enter sampling probe, the nook closing member front end is than the long 1mm of sampling probe.
The thickness of array assignment plate is identical with the sampling probe front end length.Array assignment plate and preparing instrument are movable the installation, desirablely go out horizontal positioned, make the hole towards last to make things convenient for the placement of sampling probe.
The number of sampling probe is identical with the organization chip number of arrays.
Technological merit of the present invention is: (1) concentrates sampling, arrangement chip array, need not make blank acceptor wax block, does not need drilling process, does not need single base point sample one by one, and all sample point samples and one step of organization chip moulding finish; (2) concentrate sampling respectively, in the manufacturing process of an organization chip, each sampling probe is only got once/kind of sample, has avoided the mutual pollution between tissue; (3) in the point sample process, sampling probe does not enter paraffin, and just this sends into paraffin simultaneously with institute's sampling, and point sample and one step of chip moulding finish, and more present single base circulation repetitive operation method is simple and convenient; (4) the organization chip preparing instrument prepares easyly, can not only reduce the technology cost, can also improve work efficiency and chip quality, helps penetration and promotion.
Description of drawings
Fig. 1 is the sampling instrument structural representation;
Fig. 2 is sampling probe and nook closing member structural representation;
Fig. 3 is an organization chip preparation facilities structural representation;
Fig. 4 places the preceding preparing instrument structural representation of array assignment plate;
Fig. 5 is the structural representation of the plate of arranging of sampling probe of having arranged.
Embodiment:
The invention will be further described below in conjunction with accompanying drawing.
Embodiment 1
Referring to Fig. 1-Fig. 5, the organization chip preparation facilities mainly is made up of sampling instrument I and preparing instrument II, sampling instrument I mainly comprises control depression bar 1, push rod 2, guide runner 3, hold-doun nut 4, the sampling probe 5 of band nook closing member 11, recoil press spring 6, bolt 7, locating piece 8, guiding slide bar 9, pedestal 10, guiding slide bar 9 is set on the pedestal 10, recoil press spring 6 is connected with guide runner 3, hold-doun nut 4 is set on the guide runner 3, sampling probe 5 is fixing with guide runner 3 by hold-doun nut 4, guide runner 3 is connected with an end of push rod 2, the other end of push rod 2 is connected with control depression bar 1, control depression bar 1 flexibly connects with the top of guiding slide bar 9, during following pressure-controlled depression bar 1, driving guide runner 3 by push rod 2 glides, drive sampling probe 5 and enter the marker samples sampling, bolt 7 is connected with guiding slide bar 9 by locating piece 8, glide highly with control guide runner 3, guarantee that sampling accurately, preparing instrument II mainly comprises control bolt 12, fixed head 13, nook closing member control panel 14, array assignment plate 15, L type baffle plate 16, guide pole 17, base 18,2 guide poles 17 are set on the base 18, on the top of guide pole 17 fixed head 13 is set, control bolt 12 passes fixed head 13 and is connected with nook closing member control panel 14, driving nook closing member control panel 14 by turn control bolt 12 moves up and down, nook closing member control panel 14 embeds guide pole 17 by the lateral grooves activity, nook closing member control panel 14 can be moved up and down along guide pole 17, and array assignment plate 15 places the rectangular channel top of being made up of L type baffle plate 16 and base 18.
Also can open the diameter groove suitable mutually in the side that guide runner 3 is provided with hold-doun nut 4 with sampling probe 5 diameters, fixing with hold-doun nut 4 again after will sampling probe 5 embedding.
Diameter and the sampling probe 5 point diameters hole of suitable array arrangement mutually is set on the array assignment plate 15, the thickness of array assignment plate 15 is identical with sampling probe 5 front end length, make the tail end of sampling probe 5 and nook closing member 11 not enter the hole of array assignment plate 15, when turn control bolt 12 moves down nook closing member control panel 14, just the sample tissue post is pushed among the paraffin by nook closing member 11, and guarantee that sampling probe 5 does not enter paraffin, avoid polluting sample.Array assignment plate 15 be movable installation with preparing instrument II, desirablely goes out horizontal positioned, make the hole towards last to make things convenient for the placement of sampling probe 5.
Embodiment 2
With reference to Fig. 1-Fig. 2, behind routine techniques marker samples wax stone, the sample wax stone of mark is positioned on the pedestal 10 of sampling instrument I, position by bolt 7 stationary positioned piece 8 on guiding slide bar 9, one piece of sampling probe 5 is fixed on guiding by hold-doun nut 4 to be drawn on the piece 3, following pressure-controlled depression bar 1, to lead by push rod 2 and to draw a piece 3 and move down, make sampling probe 5 front ends enter the sample wax stone, nook closing member 11 can be by getting tissue samples jack-up, the height of nook closing member 11 jack-up equals the height of the sample post got, decontrols control depression bar 1, utilizes recoil press spring 6, lift sampling probe 5, finish primary sample,, will get excellent sampling probe 5 together with by the position preface of the nook closing member 11 of jack-up according to chip design with reference to Fig. 5, being arranged in chip array arranges in the plate 15, repeat previous action, finish the concentrated sampling of one group of tissue samples and arrange, then array assignment plate 15 is placed 4 ℃ of refrigerators to place 20 minutes; With reference to Fig. 3,16 combinations of two L type baffle plates are put on the base 18 of preparing instrument II, form rectangular channel, in this rectangular channel, fill with whiteruss, placing the rectangular channel top through freezing array assignment plate 15, turn control bolt 12, promote nook closing member control panel 11 downwards, extruding nook closing member 11 tail ends once inject all tissue samples the paraffin of rectangular channel simultaneously, after treating that paraffin solidifies fully, remove array assignment plate 15, remove L type baffle plate 16, the chip wax stone is taken off the preparation of promptly finishing organization chip.
Embodiment 3
Different according to test objective and chip design can be selected the different sampling diameters and the chip array of different densities.Native system can supporting all size sampling probe (regular size is 0.6mm, 1.0mm, 1.5mm), and supporting with it by the array assignment plate of respective aperture respectively.
Embodiment 4
Sample diameter at present commonly used is 0.6mm, and conventional chip density is generally 60-100.The native system at present organization chip maximal density of the 0.6mm diameter of design is 11 * 17, if sample number is not enough 187, can select regular space-number hole to arrange, if device manufacturing process allows, chip density still has the space of further raising.
Claims (8)
1. organization chip preparation facilities, it is characterized in that: mainly form by sampling instrument (I) and preparing instrument (II), sampling instrument (I) mainly comprises control depression bar (1), push rod (2), guide runner (3), hold-doun nut (4), the sampling probe (5) of band nook closing member (11), recoil press spring (6), bolt (7), locating piece (8), guiding slide bar (9), pedestal (10), guiding slide bar (9) is set on the pedestal (10), recoil press spring (6) is connected with guide runner (3), hold-doun nut (4) is set on the guide runner (3), sampling probe (5) is fixing by hold-doun nut (4) and guide runner (3), guide runner (3) is connected with an end of push rod (2), the other end of push rod (2) is connected with control depression bar (1), control depression bar (1) flexibly connects with the top of guiding slide bar (9), sampling probe (5) is nested with nook closing member (11) activity, bolt (7) is connected with guiding slide bar (9) by locating piece (8), preparing instrument (II) mainly comprises control bolt (12), fixed head (13), nook closing member control panel (14), array assignment plate (15), L type baffle plate (16), guide pole (17), base (18), guide pole (17) is set on the base (18), on the top of guide pole (17) fixed head (13) is set, control bolt (12) passes fixed head (13) and is connected with nook closing member control panel (14), nook closing member control panel (14) flexibly connects with guide pole (17), guide pole (17) embeds nook closing member control panel (14) by groove, and array assignment plate (15) places the rectangular channel top of being made up of L type baffle plate (16) and base (18).
2. organization chip preparation facilities according to claim 1, it is characterized in that: sampling probe (5) and nook closing member (11) all have a tail end that expands, nook closing member (11) front end is inserted in the sampling probe (5), and nook closing member (11) tail end slightly larger in diameter is in sampling probe (5) tail end diameter.
3. organization chip preparation facilities according to claim 1 and 2 is characterized in that: the front end of nook closing member (11) is than the long 1mm of sampling probe (5).
4. organization chip preparation facilities according to claim 1 and 2 is characterized in that: the thickness of the array arrangement utmost point (15) is identical with sampling probe (5) front end length.
5. organization chip preparation facilities according to claim 1 is characterized in that: diameter and sampling probe (5) the point diameter hole of suitable array arrangement mutually is set on the array assignment plate (15).
6. organization chip preparation facilities according to claim 1 is characterized in that: array assignment plate (15) is installed for movable, desirablely goes out horizontal positioned.
7. organization chip preparation facilities according to claim 1 is characterized in that: the number of sampling probe (5) is identical with the organization chip number of arrays.
8. utilize the arbitrary described organization chip preparation facilities of claim 1-7 to prepare the method for organization chip, after the routine techniques marker samples, take a sample by sampling probe, it is characterized in that: concentrate sampling by the sampling instrument (I) in the organization chip preparation facilities earlier, to have the position preface of one group of sampling probe (5) of sample then according to chip design, be arranged in successively on the array assignment plate (15), then array assignment plate (15) was placed 20 minutes in 4 ℃ of refrigerators, paraffin is filled with in the rectangular channel that is combined into by two L type baffle plates (16), array assignment plate (15) is put into the preparing instrument (II) of organization chip preparation facilities, turn control bolt (12), driving nook closing member control panel (14) presses down, array assignment plate (15) is gone up all tissue samples once injects paraffin simultaneously, treat that paraffin solidifies fully after, take off the chip wax stone and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100256457A CN1306258C (en) | 2004-06-22 | 2004-06-22 | Process for preparing tissue chips and apparatus thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100256457A CN1306258C (en) | 2004-06-22 | 2004-06-22 | Process for preparing tissue chips and apparatus thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1595097A CN1595097A (en) | 2005-03-16 |
| CN1306258C true CN1306258C (en) | 2007-03-21 |
Family
ID=34663755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100256457A Expired - Fee Related CN1306258C (en) | 2004-06-22 | 2004-06-22 | Process for preparing tissue chips and apparatus thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1306258C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1920558B (en) * | 2005-12-27 | 2013-06-05 | 胡苹 | Wax module for organizing chip array |
| CN101187604B (en) * | 2007-12-14 | 2011-06-15 | 大连医科大学 | Freezing and paraffin embedded tissue chip production device |
| CN101430324B (en) * | 2008-12-08 | 2013-01-23 | 中国人民解放军第三军医大学 | Production apparatus for blank acceptor wax block of porous tissue chip |
| CN101556224B (en) * | 2009-05-18 | 2011-02-16 | 复旦大学附属中山医院 | Manufacturing method of paraffin tissue chip |
| CN101738338B (en) * | 2009-12-29 | 2012-07-04 | 李从善 | Method for manufacturing tissue array paraffin block |
| CN102042922B (en) * | 2010-11-02 | 2012-07-04 | 浙江省台州医院 | Method for making once-forming tissue chip and mold applied to same |
| CN104535751B (en) * | 2015-01-23 | 2016-08-24 | 胡苹 | Organization chip receptor blank wax stone preparing instrument and using method thereof |
| CN106769174B (en) * | 2016-11-22 | 2018-07-13 | 南京林业大学 | Plant tissue slice receptor blank wax stone boring instrument |
| CN113759143B (en) * | 2021-09-01 | 2024-04-26 | 广州耐确医疗器械有限责任公司 | Novel full-automatic tissue chip instrument |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001042796A1 (en) * | 1999-12-13 | 2001-06-14 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health | High-throughput tissue microarray technology and applications |
| WO2003067256A2 (en) * | 2002-02-05 | 2003-08-14 | University Of Medicine & Dentistry Of New Jersey | Systems for analyzing microtissue arrays |
| CN2589960Y (en) * | 2002-12-24 | 2003-12-03 | 李玉松 | Tissue chip sampler |
| CN2722226Y (en) * | 2004-06-30 | 2005-08-31 | 浙江大学 | Producer of organic chip |
-
2004
- 2004-06-22 CN CNB2004100256457A patent/CN1306258C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001042796A1 (en) * | 1999-12-13 | 2001-06-14 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health | High-throughput tissue microarray technology and applications |
| WO2003067256A2 (en) * | 2002-02-05 | 2003-08-14 | University Of Medicine & Dentistry Of New Jersey | Systems for analyzing microtissue arrays |
| CN2589960Y (en) * | 2002-12-24 | 2003-12-03 | 李玉松 | Tissue chip sampler |
| CN2722226Y (en) * | 2004-06-30 | 2005-08-31 | 浙江大学 | Producer of organic chip |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1595097A (en) | 2005-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1306258C (en) | Process for preparing tissue chips and apparatus thereof | |
| DE60028352T2 (en) | INSTRUMENT FOR MANUFACTURING TISSUE ARRAYS | |
| CN2722226Y (en) | Producer of organic chip | |
| WO2006102312A2 (en) | Microarrayer with coaxial multiple punches | |
| RU2007112508A (en) | FACILITY COLLECTOR WITH ADJUSTABLE PALLET | |
| CN103439163A (en) | Preparation method and preparation apparatus for typography type tissue chip | |
| CN1920558A (en) | Wax module for organizing chip array | |
| KR20010098101A (en) | An apparatus and method for microarrays of tissue sample | |
| CN201583439U (en) | Petrolin tissue chip paraffin block manufacture equipment | |
| CN102042922B (en) | Method for making once-forming tissue chip and mold applied to same | |
| CN205950373U (en) | Extrusion anchor possesses numerical control machining apparatus | |
| CN104535751A (en) | Tissue chip receptor blank wax block preparation device and using method thereof | |
| CN219738901U (en) | Scanning electron microscope for observing microscopic morphology of silica gel array | |
| CN213195480U (en) | Clamping base for machining stainless steel precision forging | |
| CN201885912U (en) | Mold for manufacturing tissue chip with one-step molding | |
| CN114347318B (en) | Butyronitrile glove production hand die holder convenient for hand die installation and installation method | |
| CN205898488U (en) | Tissue chip sampler | |
| CN108088729A (en) | Organization chip producing device and method | |
| CN212451437U (en) | Nucleic acid extraction appearance displacement system with perpendicular guide pillar | |
| CN1986247A (en) | Universal shaping method for cursive script potted landscape | |
| CN208420502U (en) | A kind of device making one-pass molding organization chip acceptor wax block | |
| CN217237424U (en) | Tissue chip making devices | |
| CN209784024U (en) | Device for manufacturing tissue chip receptor wax block | |
| CN221328578U (en) | Cable laying support | |
| CN205258468U (en) | Fungi monospore picking device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070321 Termination date: 20110622 |