CN1304041A - Test strip for blood-sugar and its preparation method - Google Patents
Test strip for blood-sugar and its preparation method Download PDFInfo
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- CN1304041A CN1304041A CN 99119827 CN99119827A CN1304041A CN 1304041 A CN1304041 A CN 1304041A CN 99119827 CN99119827 CN 99119827 CN 99119827 A CN99119827 A CN 99119827A CN 1304041 A CN1304041 A CN 1304041A
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Abstract
A test strip for blood sugar includes a substrate, a reference electrode and a working electrode; a sampling working zone connected with the working electrode and a medium connecting reference electrode and sampling working zone, in which the surface of sampling working zone is spray-coated with biological element which possesses effective quantity and can be mutually reacted with glucose to produce electronics. Said invention also describas a preparation method of the said test strip, and its whole production process can be controlled by a computer so as to ensure reproducibility, consistency and test accuracy of test strips.
Description
The present invention relates to a kind of family expenses blood sugar test instrument and preparation method thereof, relate more specifically to a kind of test strip for blood-sugar and preparation method thereof.
Show that according to the Ministry of Public Health 97 years investigation the onset diabetes rate of China surpasses 2,000 ten thousand, and annually also just increase with 1.5% speed of development.One of effective means of control of diabetes development is the long-term correct test of patient's blood sugar and is aided with medicine and diet control.Recently the miniature blood glucose meter and the disposable electrode test bar that utilize the optical reflection principle to make that occur cooperate, and only need can measure patient's blood-sugar content once bleeding in one minute.The test philosophy of this test-strips adopts colorimetric method, and the tester external form is heavy, and patients'blood need enter people's instrument middle part, thereby forms cross pollution easily.And the complex manufacturing technology of this test-strips needs special material, thereby cost and rejection rate are all higher, are difficult to realize at home industrialization.
An object of the present invention is to provide a kind of test-strips of utilizing electrochemical principle to test blood sugar.
Another object of the present invention provides the preparation method of this test-strips.
To be specifically described as the technical solution of the present invention that realizes these purposes below.
The invention provides a kind of test-strips that is used to test blood sugar, this test-strips comprises: a substrate; A reference electrode; A working electrode; The application of sample workspace that links to each other with working electrode and is connected reference electrode and the medium of application of sample workspace, wherein sprayed on the application of sample workspace effective dose can with the react to each other biological elements of final generation electronics of glucose.
The present invention also provides the preparation method of this test strip for blood-sugar, and this method comprises: with method for printing screen reference electrode and working electrode are printed on the basalis, then biological elements is sprayed on the application of sample workspace.
Fig. 1 is a silk-screen thick membrane electrode process schematic representation of the present invention.
Fig. 2 is the synoptic diagram of spraying printing biological elements among the present invention.
Fig. 3 is the synoptic diagram of test strip for blood-sugar of the present invention.
Below in conjunction with accompanying drawing the present invention is described in further detail.
The used substrate of test-strips of the present invention can be any nonconducting, stable material mechanically. This A little materials for example are various polyester, comprise poly-ethyl ester. Reference electrode and working electrode can be with conventional conductive material systems Become, these materials for example are silver/silver oxides, platinum/carbon etc. The front end of working electrode is the application of sample workspace. Reference Conducting medium between electrode and the application of sample workspace can be some materials that can conduct electricity, wherein better material Be benzoquinones and the potassium ferricyanide, best is benzoquinones. The biological elements that is sprayed on the application of sample workspace can be and the Portugal The reaction of grape sugar and the final material that produces electronics, for example glucose oxidase or GDH.
When measuring blood sugar, glucose and glucose oxidase enzyme reaction produce gluconic acid and hydrogen peroxide, simultaneously Produce electronics, just can record concentration of glucose in the blood sample by measuring size of current.
The present invention also provides the preparation method of this test strip for blood-sugar, and the method adopts thick film silk-screen and spraying technology Combine, stamp the thick-film conductor carbon electrode with screen process press at poly-ethyl ester (PE) substrate earlier, use then precision The spraying instrument sprays to biological elements on the electrode workspace. In said method, used screen printer and accurate spray Be coated with instrument and control by computer, thereby make the uniformity, repeatability, measuring accuracy etc. of the test strip for blood-sugar that makes big The big raising, this has not only simplified technology, has reduced cost, and percent defective is reduced greatly.
Embodiment:
To gather the ethyl ester substrate, to be cut into 150 * 150 centimeters sheet materials in advance standby.
Add 1.2% hydroxyethyl cellulose in 1 gram dag, add the conductive material benzoquinones of appropriate amount, mixing is standby.The nylon matter net material of suitable order number is stretched tight on the square light-weight metal square frame of 250 * 250mm, in the darkroom, coat photoresists, drying for standby.The electrode model film of computer drawing is placed on the photoresists, exposed 15 minutes down in uviol lamp.Wash the unhardened sensitive emulsion that is hidden by electrode template only with hydraulic giant.Dry back becomes the silk-screen template.The silk-screen template is installed on the automatic screen printer, adds electrocondution slurry behind adjustment spacing and the pressure.Make base electrode, printed dry back standby.
The thick film silk-screen electrode that prints is held on 60-100 ℃ to be toasted 30-90 minute down.
In addition, glucose oxidase or the glucose dehydrogenase of activity more than 250u/mg allocated in the phosphate buffer, made the biological elements spray coating liquor.Spray coating liquor is added in the reservoir of spraying instrument,, spray coating liquor is sprayed directly on the workspace of thick membrane electrode then, make the electric pole type test strip for blood-sugar by computer installation spacing and drop size.Make the test-strips that makes under 30% relative humidity drying at room temperature 4-8 hour.
At last, under 30% relative humidity and room temperature, the test-strips that the test-strips of drying is cut into 3.5 * 0.8 sizes is standby with shearer.
Claims (2)
1. test-strips that is used to test blood sugar, this test-strips comprises: a substrate; A reference electrode; A working electrode; The application of sample workspace that links to each other with working electrode and is connected reference electrode and the medium of application of sample workspace, wherein sprayed on the application of sample workspace effective dose can with the react to each other biological elements of final generation electronics of glucose.
2. the preparation method of a test strip for blood-sugar as claimed in claim 1, this method comprises: with method for printing screen reference electrode and working electrode are printed on the basalis, then biological elements is sprayed on the application of sample workspace.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99119827 CN1304041A (en) | 1999-10-25 | 1999-10-25 | Test strip for blood-sugar and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99119827 CN1304041A (en) | 1999-10-25 | 1999-10-25 | Test strip for blood-sugar and its preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1304041A true CN1304041A (en) | 2001-07-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99119827 Pending CN1304041A (en) | 1999-10-25 | 1999-10-25 | Test strip for blood-sugar and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1304041A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100380123C (en) * | 2001-08-01 | 2008-04-09 | 生命扫描有限公司 | Method and apparatus for measuring concentration of analyte |
| CN102395884A (en) * | 2009-03-23 | 2012-03-28 | 红电医学科技股份有限公司 | Fluid test chip and method to make it |
| US9518951B2 (en) | 2013-12-06 | 2016-12-13 | Changsha Sinocare Inc. | Disposable test sensor with improved sampling entrance |
| US10571425B2 (en) | 2013-05-09 | 2020-02-25 | Changsha Sinocare Inc. | Disposable test sensor with improved sampling entrance |
-
1999
- 1999-10-25 CN CN 99119827 patent/CN1304041A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100380123C (en) * | 2001-08-01 | 2008-04-09 | 生命扫描有限公司 | Method and apparatus for measuring concentration of analyte |
| CN102395884A (en) * | 2009-03-23 | 2012-03-28 | 红电医学科技股份有限公司 | Fluid test chip and method to make it |
| US10571425B2 (en) | 2013-05-09 | 2020-02-25 | Changsha Sinocare Inc. | Disposable test sensor with improved sampling entrance |
| US9518951B2 (en) | 2013-12-06 | 2016-12-13 | Changsha Sinocare Inc. | Disposable test sensor with improved sampling entrance |
| US10088444B2 (en) | 2013-12-06 | 2018-10-02 | Changsha Sinocare Inc. | Disposable test sensor with improved sampling entrance |
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| C06 | Publication | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |