CN100451637C - Electrochemistry type sensor and its manufacturing method - Google Patents

Electrochemistry type sensor and its manufacturing method Download PDF

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CN100451637C
CN100451637C CN 03102988 CN03102988A CN100451637C CN 100451637 C CN100451637 C CN 100451637C CN 03102988 CN03102988 CN 03102988 CN 03102988 A CN03102988 A CN 03102988A CN 100451637 C CN100451637 C CN 100451637C
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黄椿木
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黄椿木
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本发明为一种电化学式传感器(Electrochemical Sensor)及其制造方法,其制造方法包含:提供一射出成型装置(模具),提供一绝缘基材,其具有一第一凹槽及一第一贯孔,将该绝缘基材送入该射出成型装置内,注入一塑胶导电的成型材料至该射出成型装置内,以于该第一凹槽内形成一第一导电条(ConductiveStrip),而该第一导电条具有一第一输出端及相对的一第二电极端,且以该第二电极端为一第一测试电极,该第一测试电极即于该第一贯孔中形成,以及提供一化学试剂(Reagent),其设置于该第一测试电极上,用以检测一流体样本,该化学试剂即与该流体样本之一成分反应,进而产生一测量信号,并经该第一测试电极以输出该测量信号。 The present invention is an electrochemical sensor (Electrochemical Sensor) and a manufacturing method, the manufacturing method comprising: providing an injection molding apparatus (mold), providing an insulating substrate having a first recess and a first through hole , the insulating base material fed into the injection molding machine, injection molding a plastic material into the electrically conductive inner injection molding apparatus to form a first conductive strip (ConductiveStrip) within the first recess, and the first a conductive strip having a first output terminal and a second electrical terminal opposite to, and at the second electrical terminal is a first test electrode, i.e., the first test electrode formed on the first through hole, and providing a chemically reagent (reagent), which is disposed on the first test electrode, for detecting a fluid sample, i.e., the chemical agent is reacted with one of the components of the fluid sample, thereby generating a measurement signal, and by outputting the first test electrode the measurement signal.

Description

电化学式传感器及其制造方法 Electrochemical sensor and its manufacturing method

(1) 技术领域 (1) Technical Field

本发明为一种电化学式传感器及其制造方法。 The present invention is an electrochemical sensor and a manufacturing method.

(2) 背景技术 (2) Background Art

一般电化学传感器(Electrochemical Sensor)的原理,于数十年来已被成熟应用于各种流体的生化成分检测上,而在一实验室或专业的检验室中的一电化学检测的装备,因不同检验功能需求可有不同的配备。 General principles of electrochemical sensors (Electrochemical Sensor), and in the decades has been applied to mature on various fluids to detect biochemical components, in a laboratory or in a professional laboratory equipped with a electrochemical detection, depending inspection functional requirements may have different equipment. 请参阅图1,其显示出一电化学检测装置10的最基本架构为- Please refer to FIG. 1, which shows the basic architecture of an electrochemical detection apparatus 10 is -

1. 容置一待测流体ll的一承装容器12,以为电化学的测量区域13。 1. ll accommodating a fluid to be measured in a holding container 12, that the measurement of the electrochemical area 13.

2. —化学试剂(Reagent) 14,用于与待测流体11的一分析物产生一化学作用, 并产生一电性参数的输出信号,此电性参数的输出值与待测流体11的该分析物的生化成分有关。 2. - the output signal Chemicals (Reagent) 14, for generating a chemical reaction with the analyte in a fluid to be measured 11, and generates an electrical parameter, this output value of the electrical parameters of the fluid to be measured with 11 about the biochemical composition of the analyte. 例如待测流体11为人的血液,分析物为血醣的浓度,则此化学试剂14基本上为会为一葡萄糖氧化酶及其他复合物。 11 fluid to be measured, for example, human blood, the analyte is blood glucose concentration, this chemical agent 14 will essentially be a composite of other enzymes glucose oxidase.

3. 二至三个测试电极,如15对电极(Counter Electrode) ,16工作电极(Working Electrode) ,17参考电极(Reference Electrode),用于将一电化 3. tests two to three electrodes, such as 15 pairs of electrodes (Counter Electrode), 16 of the working electrode (Working Electrode), 17 the reference electrode (Reference Electrode), for a galvanic

学作用所需的一工作电压由一计量器18引接至化学反应容器12,并将待测流体11 的该分析物经一电化学作用后的该电性参数输出信号电流,引接至计量器18作一数值分析及显示。 Required to learn a role of the operating voltage by the connection of a meter 18 to a chemical reactor vessel 12, and after a test of the electrochemically active electrical parameter of an output signal current of the analyte fluid 11, to the connection of the gauge 18 and a display for a numerical analysis.

4. 电化学计量器18,用于提供该电化学作用所需的工作电压(或电流),并量取该电化学作用后的该电性参数的输出信号电流(或电压),作成一记录与该数值分析及显示测试数据。 The output signal current of the electrical parameters of the electrochemical 4. gauge 18 for providing the desired effect of the electrochemical voltage (or current), and the amount of the electrochemically active (or voltage), creating a record test data analysis and display of this value.

其中的测试电极15,16,17可只使用二个电极,其为对电极(Counter Electrode)与工作电极(Working Electrode)。 Wherein the test electrodes may be only two electrodes 16, 17, which is a pair of electrodes (Counter Electrode) and the working electrode (Working Electrode). 或可使用三个电极,除工作电极与对电极外,另一为参考电极(Reference Electrode)。 Or three electrodes may be used, in addition to the working electrode and the counter electrode and the other as a reference electrode (Reference Electrode). 或可再加上第四个以 Or can be combined with the first four

上的检测电极(Detecting Electrode),因电化学作用需求不同可调配不同电极数目。 Detection electrodes (Detecting Electrode), because of the different needs of electrochemical action may deploy different number of electrodes.

除了电极数目的不同外,因不同的电极功能(如counter electrode相对于working electrode的不同)与传感器化学反应成分的不同,各电极材质的选用可有非常大的变化。 In addition to the different number of electrodes, because the different functions of the electrode (e.g., counter electrode with respect to various working electrode) is different from the chemical reaction of components of the sensor, the electrodes may have a choice of material a very big change. 实验室通用的电极材料为如下,其中对电极15 (Counter Electrode)材料只要是导电的材质即可,但导电电阻愈低愈好,如铜棒、银棒、 镍棒、石墨棒、或以碳胶或银胶印刷成形的导电膜电极,当然金、白金等贵金属也可用于当对极电极材料,只是浪费材料成本。 General Laboratory electrode material are as follows, wherein the counter electrode 15 (Counter Electrode) as long as the material is electrically conductive material can be, but the smaller the better conduction resistance, such as copper, silver rod, a nickel rod, graphite rods, or carbon electrode paste or a conductive film formed of silver glue printing, of course, gold, platinum and other precious metals may also be used as material for electrode, a waste of material costs. 其中参考电极17 (Reference Electrode)的材质最常用为一银/氯化银修饰电极(Modified Electrode) 171, 其为在银棒、或以碳胶或银胶所印刷的导电膜电极上,各再印刷一层氯化银或利用电解方式镀上一层氯化银。 Wherein the reference electrode 17 (Reference Electrode) The material most commonly used is a silver / silver chloride electrode modified (Modified Electrode) 171, which is on the silver rod or a conductive film electrode silver paste or carbon paste printed, then each printing a layer of silver chloride or with electrolytically plated with a layer of silver chloride.

其中工作电极16 (Working Electrode)的电极材料的选用更为复杂,其有两大方向,第一类为金属催化电极(Metal-Catalyzed Electrode),其选用的工作电极材料对分析物会产生化学催化作用或对分析物具有单一选择性,此具有催化作用或单一选择性的特点使测量信号有更稳定或检测信号更放大的效果,其金属电极材质通常为如金、白金、钯,铑等贵金属(如铑rhodium电极对H202有极佳的催化作用)。 Wherein the working electrode material selection electrode 16 (Working Electrode) is more complex, which has two directions, the first type of metal catalyst electrode (Metal-Catalyzed Electrode), the working electrode material which is selected analyte produces chemical catalysis a single action or selective for the analyte, this characteristic having a single selective catalytic or more stable the measurement signal or the detection signal is more amplified effect, the electrode is usually made of metal such as gold, platinum, palladium, and rhodium (rhodium such as rhodium electrodes have excellent catalytic H202). 第二类为利用电子转移媒介物的修饰工作电极(Electron-Transfer Mediator Modified Working Electrode),此电子转移媒介物一般为一复合物,其由多件化学原料混合的试剂,将此化学试剂固化(immobilized)在工作电极的表面,可与待测分析物产生电化学反应,而将电化学反应后产生的电性信号经由电极输出其测量参数,此工作电极的目的只作为传导功用,其电极材料需不可与待测流体11或化学试剂14产生一自我化学作用而干扰测量准确性。 The second category is the use of modified electron transfer mediator to the working electrode (Electron-Transfer Mediator Modified Working Electrode), this electron transfer mediator is typically a composite material consisting of a multi-piece mixing chemical reagents, this solidified chemical agent ( Immobilized) the surface of the working electrode, and an electrochemical reaction occurs can analyte to be measured, and the electrical signal generated after the electrochemical reaction which measures the output parameter via the electrode, the working electrode only purpose of this function as a conductive, the electrode material need not be a self-generating chemical reaction with the fluid to be measured 11 14 Chemicals or interfere with measurement accuracy. 此无化学干扰的电极材料为一惰性导电材料, 一般为惰性金属Noble Metal (如金、 白金、钯palladi咖、铑rhodiutn等金属)或含碳材质的电极(如碳膜印刷电极Carbon Base Screen Printing Electrode、石墨棒Graphite Bar)。 This electrode material for a non-interfering chemically inert conductive material, typically an inert metal Noble Metal (such as gold, platinum, palladium palladi coffee, a metal such as rhodium rhodiutn) electrode or a carbonaceous material (e.g., carbon-printed electrode Carbon Base Screen Printing Electrode, graphite rod graphite bar). 因碳与惰性金属在低温下不会起化学作用,故不会产生化学干扰。 Inert metal by carbon at low temperatures without chemical reaction, chemical interference is not generated.

本发明并不在于解决电极材质问题,而是在应用已发展成熟的各种不同需求的电极材质,尤其是以低成本的含碳导电材质,以本发明的方法与结构来制造出一工作面积尺寸稳定、更低的电极电阻及无化学干扰的工作电极,可使电化学式传感器的测量信号更稳定为其目的。 The present invention is not to solve the problem of electrode material, but in the development of various applications have different needs mature electrode materials, in particular conductive carbon material at low cost, to methods and structures of the present invention to produce a work area dimensionally stable, lower electrode and the working electrode resistance without chemical interference, electrochemical sensors make a measurement signal for the purpose of more stable.

于图1中显示出一种电化学式传感器的基本架构,其中电极可用二电极式或 Shown in FIG. 1 the basic structure of an electrochemical sensor, wherein the two-electrode or electrodes may be

三电极式,其电路测量结构置放于计量器18的装置内。 A three-electrode, the circuit structure of the measurement apparatus is placed in the meter 18. 由计量器18供给一固定的电压值E经导线输出至电极15, 16, 17以为待测流体分析物11与化学试剂14的电化学工作电压,待分析物与试剂化学反应后由此工作电压产生一扩散信号电流i,此测量的信号电流i与分析物的浓度C (Concentration of the analyte)有一比例的关系,其中i与C的电化学公式,因化学作用为可逆、准可逆或不可逆的不同,或化学试剂为为可溶解或不可溶解,或因电极形状为平板、圆柱或其是一尺寸小于50 um的微电极(Micro-electrode )的不同,其适用的电化学公式如Cott.rell equation或Sampled-current voltammetry equation、等各不同, 但基本上综合为如下: After the meter 18 is supplied from a fixed voltage value E is output to the electrode via a wire 15, 16, 17 that the operating voltage of electrochemical analyte fluid to be measured 11 with the chemical 14, the analyte and the reagent chemical reaction whereby the operating voltage generating a spread signal current i, this measured concentration C signal current i and the analyte (concentration of the analyte) have the relationship a ratio, wherein the electrochemical formula i and C, due to chemical action is reversible, quasi-reversible or irreversible different, or chemical agents are soluble or is insoluble, or because the shape of the electrode plate, a cylinder or a different size microelectrodes (micro-electrode) is less than 50 um, which is suitable for electrochemical formula as Cott.rell equation or Sampled-current voltammetry equation, and other different, but substantially integrated into the following:

i (t) = n. FA f (C) . f (D) . f (X) . f ") i (t) = n. FA f (C). f (D). f (X). f ")

其中i: 测量电流值(Measure current) Where i: a current measurement value (Measure current)

n: 电子价数(Number of transfer electrons ) F:法拉第常数(Faraday constant) n: number of electrons (Number of transfer electrons) valence F: Faraday constant (Faraday constant)

A:工作电极的表面积(Surface area of the working electrode) D:扩散常数(Diffusion coefficient) C:待测分析物的浓度(Concentration of the analyte ) X:化学试剂的厚度(Thickness of the reagent) t: 测量时间(Time from start measure) 测量电流i与n、 F、 A为一线性正比关系,但其与分析物的浓度C或化学试剂厚度X在各种不同状况下,有些是线性关系但有些是非线性关系,故以函数关系f (C) 、 f (X)表示。 A: surface area of ​​the working electrode (Surface area of ​​the working electrode) D: diffusion constant (Diffusion coefficient) C: concentration of the analyte to be measured (Concentration of the analyte) X: thickness of chemicals (Thickness of the reagent) t: measuring time (time from start measure) and measure the current i n, F, a is a linear proportional relationship, but the concentration of a chemical agent or a thickness X C with the analyte under various conditions, some are non-linear, but some a linear relationship, so as to function f (C), f (X) represents. 另多数测量电流为一非稳态值,其为时间t的函数以f (t)表示。 Most of the other is a non-steady-state current measured value, which is a function of time t is expressed in f (t).

此外若电化学测量结构为两电极式电路,则由计量器18供给的固定电压E,与实际的电极工作电压为V为不相等,其中V会随信号传递压降而变化。 Furthermore electrochemical measurement structure when supplied two-electrode circuit, the meter 18 by the fixed voltage E, and the actual operating voltage of the electrode is not equal to V, where V varies with the pressure drop signal transmission. V = E - LRV:实际的电极工作电压E:计量器提供的固定电压值 V = E - LRV: the actual operating voltage electrodes E: fixed voltage value provided by meter

1:测量电流值 1: measured current value

r:电极与电极导引线(lead)的总阻抗值 r: wire electrode and the electrode guide (Lead) The total impedance value

i. r:信号传递电压降 i r:. Signaling voltage drop

由以上两公式的学理数据知,虽化学反应是决定传感器电流测量值大小的主 Theoretical data known from the above two formulas, although a chemical reaction is to determine the measurement value of the main sensor current magnitude

要因素,但除化学反应的影响外,属于电极的结构部分的电极工作表面积a与电极的总阻抗值r的稳定性皆是影响传感器大量生产时测量信号品质稳定的重要因 To factors, but in addition to the effects of chemical reactions, the total resistance value r of the stability of the surface area of ​​a working electrode and an electrode which are part of the electrode structure are all important factor when the measured signal quality and stability of the impact sensor mass production

素。 Su. 另化学试剂的涂布厚度均匀稳定亦是一要素。 Another chemical agent of coating thickness uniformity and stability is also a feature.

纯惰性金属所作成的电极于电极的工作表面积a与电极的总阻抗值r皆有极佳的效果,但其成本高。 Pure inert metal electrodes made into the total resistance value r of a working surface area of ​​the electrode and the electrode Jie excellent results, but its high cost. 而低价的传统网版印刷导电膜式电极于此两要素有一定程度的缺点。 And low-cost conventional screen printing a conductive film electrode elements thereto two disadvantages to some extent. 本发明在惰性金属电极材质的外再寻求出另一低成本的解决方法, 从如何改善化学干扰及电极的工作表面积的稳定及降低电极的总阻抗值r着手, 以改善传统网版印刷电极的缺点。 The present invention is an inert metal electrode material and then to seek a further outer cost solutions, how to improve the chemical stability of the surface area of ​​the electrodes and the interference and reduce the overall electrode impedance value r to proceed to improve the conventional screen printing electrode shortcomings.

一般的实验室的泛用电极很贵,因而皆会在多次使用后才丢弃,但每做完一次实验后,电极棒表面已被待测物或化学试剂污染,甚至电极表面已被镀上一层化合物,其无法简易清洗, 一般需用研磨器具将电击棒上的污染去除才可再用。 General laboratory pan electrode expensive, and thus both will be discarded after repeated use, but after each finished experiment, the surface of the electrode rod has been tested, or chemical contamination of the reagent, and even on the electrode surfaces have been plated one compound which can not be easy cleaning, polishing tool is generally required to remove the contamination on the stun before reuse. 此过程耗材又耗时。 This process supplies and time-consuming. 故有一拋弃式印刷电极20 (详图2)的开发。 Therefore, the development of a disposable printed electrode 20 (detail 2). 此拋弃式印刷电极20,是在一平坦薄片的塑胶绝缘基材21上以一网版印刷技术将一糊状导电膏印刷成导电膜20、 22、 222、 23及印刷一绝缘层223等而成型。 This disposable printed electrode 20 is a flat sheet of plastic in the insulating base plate 21 to a network printing technique pasty conductive paste printed conductive film 20, 22, 222, 23, and an insulating layer 223 and other printed and forming. 其具有制程容易、形状易变,且成本低等优势,因而被广泛使用。 Having an easy process, variable shape, and low cost, and therefore, is widely used. 因其成本低故使用一次即拋弃,无重复使用的污染与清洗的困扰。 Due to its low cost and therefore disposable once i.e., no problems with the cleaning contaminated reusable. 此拋弃式印刷电极因成本低,形状设计容易等优点,尤其被广泛应用于居家医疗的电化学传感器上,如血醣、尿酸、胆固醇的检测试片电极。 This disposable electrode printing due to low cost, easy to design the shape, etc., in particular, the electrochemical sensors are widely used in home health care, such as glucose, uric acid, cholesterol test strip electrodes.

如台湾专利公告第496, 960、 496, 110、 475, 983、 466, 344、 416, 005、 374, 116、 369, 411号等,及美国专利公告第5, 985, 116、 6, 125, 292、 6, 059, 946、 6, 270, 637、 5, 997, 817、 5, 951, 836、 6, 258, 254、 5, 876, 577、 6, 413, 394、 6, 129, 823, 5, 916, 156, The Taiwan Patent Publication No. 496, 960, 496, 110, 475, 983, 466, 344, 416, 005, 374, 116, 369, 411, etc., and U.S. Patent Publication No. 5, 985, 116, 6, 125, 292, 6, 059, 946, 6, 270, 637, 5, 997, 817, 5, 951, 836, 6, 258, 254, 5, 876, 577, 6, 413, 394, 6, 129, 823, 5, 916, 156,

5, 989, 409, 6, 416, 641, 5, 437, 999, 6, 156, 173, 6, 287, 451, 5, 762, 770, 5, 628, 890, 5, 989, 409, 6, 416, 641, 5, 437, 999, 6, 156, 173, 6, 287, 451, 5, 762, 770, 5, 628, 890,

6, 436, 256号等数十件专利说明书中皆是拋弃式印刷电极的例子。 Dozens of patent specification 6, 436, 256, etc. are all examples of disposable printed electrode.

请参考图2及图3,其为美国专利第5,985, 116号的实施例的平面图与爆炸图,其为一拋弃式网版印刷电极的例子,其用糊状导电膏印刷成型的一工作电极 Please refer to FIG. 2 and FIG. 3, which is a U.S. Patent No. 5,985, plan view of the exploded view of the embodiment No. 116 is an example of a disposable screen printed electrode, a working printing a conductive paste which was formed paste electrode

(Working Electrode)22与对电极(Counter Electrode) 23是为一相互隔离的测试电极,此导电膜膜20的一第一端221为一工作电极22,经导电膜膜20连接至相对的另一侧即一第二端222,以作为工作电极22的输出端222,用于连接至一计量器18 (未显示于图上,其如图15的计量器150)。 (Working Electrode) 22 and the counter electrode (Counter Electrode) 23 was isolated as a test electrode, the conductive film 20 is a film 221 to a first end of a working electrode 22 connected to the opposite film 20 via the conductive film i.e., a side of the second end 222, to the working electrode 22 as an output terminal 222 for connection to a gauge 18 (not shown in the figure, which meter 150 in FIG. 15). 在导电膜导电膜20上具有如图3所示的一绝缘层223,用于覆盖除了电极22,23面积与输出接点222以外的区域,绝缘层30用于隔离电极以外的导电膜20与一待测流体接触。 Having an insulating layer as shown in FIG. 3223, to cover a region except the contact electrodes 22 and 23 and the output area 222, the insulating layer 30 except for the conductive film 20 and the shielding electrode in a conductive film 20 on the conductive film contacting fluid to be measured. 在绝缘层上又有一间隔器(Spacer) 31,以供该待测流体的流入,且利用一覆盖层32以形成一毛细流动通道的测量区域。 Another insulating layer on a spacer (Spacer) 31, for inflow of the fluid to be measured, and the measurement region using a capillary flow passage to form a cover layer 32.

至于该糊状导电膏的材质, 一般为导电金属粉(如金、钯、银、铜、镍等)或含碳导电材料(如碳黑、石墨粉等)与溶剂或矿石油或树酯(Resin)等binder混合成糊状的导电膏(Conductive Paste)再印刷在绝缘片上。 As for the conductive paste of the pasty material, generally a conductive metal powder (such as gold, palladium, silver, copper, nickel, etc.), or a conductive carbonaceous material (such as carbon black, graphite powder) with a solvent or mineral oil or resin ( Resin) mixing into a paste binder and other conductive paste (conductive paste) and then printed on the insulating sheet. 如采用金属膏的印刷电极,具有导电阻抗低的优点,但大部分的低价金属膏其若用于工作电极会产生化学干扰作用,而无法使用,如银材料因材质干扰问题而无法适用于血醣浓度测试片的工作电极(但银电极可用于血醣浓度测试的参考电极与对电极)。 The electrode metal paste by printing, has the advantage of low electrical impedance, but most of the low valent metal paste which will be used if the working electrode to produce a chemical interference, can not be used, such as a silver material because the material can not be applied to interference a working electrode (but the silver reference electrode can be used to test the blood glucose concentration and the counter electrode) blood glucose concentration of the test piece. 这样因而有两个选择,其一方向为用贵金属膜如金、白金、钯、铑等,另一为绝大部分用低价的导电碳膜来代替,碳与贵金属两者皆无因材质所引起的化学千扰问题, 但贵金属成本太贵,而碳导电膜会因阻抗高所引起的传导电压降会造成测量信号的误差,各为其缺点。 Such therefore have two options, one direction is a noble metal film such as gold, platinum, palladium, rhodium and the like, as most of the other low-cost electrically conductive carbon in place, both the carbon material with the noble metal are not due to the interference problems caused by chemical thousand, but the cost of expensive noble metals, and conductive carbon conductive film due to the voltage high impedance drop caused the error will cause the measurement signal, for respective disadvantages.

还有些网版印刷电极为改善前述的两个各自缺点,其先印上一层低价金属膏(如银胶)导电膜于一平板塑胶片上以获得低传导阻抗,接着在该金属粉导电膜上再印上一层导电碳膜覆盖以获得无化学干扰,而具有双重效果(如美国第6,458,258号专利的Example 1所述制程)。 Some screen printing of the electrodes to improve their two disadvantages, which paste a layer of low valent metal (e.g., silver paste) printed on the first conductive film on a flat plastic sheet to obtain a low conduction resistance, and then the metal powder in the electroconductive film and then printed on a layer of conductive carbon film to obtain an interference-free chemical, and has a dual effect (e.g., U.S. Pat. No. 6,458,258 of the Example 1 process). 但于实际的制程中,碳膜覆盖于该金属导电膜上,碳膜层常会有少许成品仍留有一部分的气孔未完全覆盖住金属导电膜,此气孔会造成该金属膏与待测流体直接接触而产生化学干扰。 But the actual manufacturing process, the carbon film covering the metal conductive film, often have little carbon layer still remains part of the finished product porosity not completely cover the metal conductive film, this will cause the pores of the metal paste and the fluid to be measured directly contacting a chemical interference. 部份为解决此如上述的网版拋弃式印刷电极的弊病,而衍生其他不同拋弃式电极的结构,如美国专利第5, 437, 999号,即以一种物理沉积法于绝缘基材上制作一贵金属薄膜, 再将之黏贴在一软或硬的基材上而成为一适合化学作用的工作电极,此法效果很好但材料成本与制程成本皆很高。 To solve the shortcomings as part of the above-described disposable screen printed electrode, while derived from various other disposable electrode structure, as described in U.S. Patent No. 5, 437, 999, i.e. in a physical deposition method on the insulating substrate the production of a noble metal thin film material, then the adhesive on a hard or soft substrate for a working electrode to become the chemistry, this method works well, but the material cost and manufacturing cost are high.

至于台湾专利第496,110号,其用一印刷电路板制作方式来制作一铜箔(加 In Taiwan Patent No. 496,110, which was a printed circuit board production methods to produce a copper foil (plus

上镀镍)电极,再于该铜箔电极上镀上一层金或白金的惰性金属。 Nickel-plated) electrodes, and then coated on an inert metal layer of gold or platinum on the electrode foil. 此种以一电镀贵金属为材质的拋弃式电极虽有其优点也降低了一部份成本,但是成本仍高。 Such disposable electrode is a noble metal plating material although it also reduces the advantage of a part of the cost, but the cost is still high. 且该印刷电路板上镀金的生产方式,仍有生产时镀层不均而造成金属膜上有残留气 And the printed circuit board plated in production, while still producing a metal plated film caused by uneven residual gas

孔,仍有一部分比率的不良成品会因残留气孔使铜膜产生电化学干扰的实务困扰, 虽其可将镀金的厚度增加以盖住全部残留气孔,但镀金太厚所造成的成本增加与 Poor finished bore, still part due to the residual porosity ratio of the copper film electrochemical interference substantive problems, although it may increase the thickness of the gold plating to cover all of the residual pores, but the cost increases caused by too thick gold plating and

使用纯贵金属导电膜的成本已几乎相同了。 Cost of using pure noble metal conductive film have almost the same.

(3)发明内容 (3) Disclosure of the Invention

因此,本发明的目的是提供一种电化学式传感器及其制造方法,以低成本的方式解决电化学式传感器测量时的电化学干扰的问题又可降低电极的电阻值,并能大幅提升测试电极的电极面积的精确度,使电化学式传感器测量信号的再现性准确度(R印roducibility)能大幅提升。 Accordingly, an object of the present invention is to provide an electrochemical sensor and a manufacturing method at low cost solution to the electrochemical interference electrochemical sensor for measuring the resistance value decreases in turn the problem of the electrode, and can significantly improve the test electrodes accuracy of electrode area of ​​the electrochemical reproducibility accuracy (R printed roducibility) sensor measurement signal can be increased significantly.

本发明的制造方法是包含:提供一射出成型装置,提供一绝缘基材,其具有一第一凹槽及一第一贯孔,将该绝缘基材送入该射出成型装置内,注入一塑胶导电的成型材料至该射出成型装置内,以于该第一凹槽内形成一第一导电条,而该第一导电条具有一第一输出端及相对一第二电极端,且以该第二电极端为一第一测试电极,该第一测试电极即于该第一贯孔中形成,以及提供一化学试剂(Reagent),其设置于该第一测试电极上,用以检测一流体样本, 该化学试剂即与该流体样本的一成分反应,进而产生一测量信号,并经该第一测试电极以输出该测量信号。 The manufacturing method of the present invention comprises: providing an injection molding apparatus, providing an insulating substrate having a first recess and a first through hole, the insulating substrate is fed into the injection molding apparatus, injecting a plastic the electrically conductive molding material into the molding apparatus is emitted to form a first conductive strip inside the first recess, and the first conductive strip having a first output terminal and a second electrical terminal opposite, and to the second two electrical terminal of a first test electrode, i.e., the first test electrode formed on the first through hole, and providing a chemical reagent (reagent), which is disposed on the first test electrode, for detecting a fluid sample the chemical agent that is reacted with a component of the fluid sample, thereby generating a measuring signal, via the first test electrode and to output the measurement signal.

较佳者,该方法的第一测试电极输出该测量信号至一计量器,且该塑胶导电材料是由一含碳导电材料或一金属粉或两种以上导电材料的混合与一树酯基材所合成的导电复合材料,该树脂基材为一热成形的塑胶树酯(Plastic Resin》 It is preferred, the first test electrode method outputs the measurement signal to a meter, and the conductive plastic material is made by mixing a carbonaceous material or a conductive metal powder or two or more electrically conductive material and a resin substrate conductive composite material synthetic resin substrate is a thermoformed plastic resin (plastic resin "

较佳者,该方法的塑胶导电材料的该含碳导电材料为一碳黑(Carbori Black)、石墨粉(Graphite Powder)、碳纤维(Carbon Fiber)或纳米碳管(Carbon Nanotube),该金属粉为一钯、铑、金等,且该导电碳材具有该塑胶基材的一3~60%的重量比,该金属粉具有该塑胶基材的一0.1~5%的重量比。 Preferably person, the carbonaceous conductive material is electrically conductive plastic material which is a carbon black (Carbori Black), graphite (Graphite Powder), carbon fiber (Carbon Fiber) or carbon nanotube (Carbon Nanotube), the metal powder is a 3 to 60% by weight of a palladium, rhodium, gold, and the conductive carbon material having a ratio of the plastic substrate, the metal powder having a 0.1 to 5% by weight of the plastic substrate ratio.

当然,该方法的塑胶导电材料的该树脂基材是可以为一环氧树酯(epoxy), Of course, the resin substrate is electrically conductive plastic material which is may be an epoxy resin (Epoxy),

其与该导电碳材或该金属粉搅拌呈一液态而注入该绝缘基材的该第一凹槽及该第一贯孔,该第一导电条即与该绝缘基材形成一紧密配接。 A liquid which was stirred with the carbon material or the conductive metal powder and injected into the insulating substrate and the first recess of the first through hole, i.e., the first conductive strips to form an intimate contact with the insulating substrate.

本发明亦包括一种电化学式传感器的结构,是用于检测一流体样本,其包含一绝缘基材,其具有一第一凹槽及一第一贯孔, 一第一导电条,是与该绝缘基材相连接,以将该第一导电条设置于该第一凹槽内,而该第一导电条具有一第一输出端及相对一第二电极端,且以该第二电极端为一第一测试电极,该第一测试电极即设置于该第一贯孔中,以及一化学试剂,其设置于该第一测试电极上,用以检测该流体样本的一成份,进而产生一测量信号,并经该第一测试电极以输出该测量信号。 The present invention also includes structure for an electrochemical sensor, for detecting a fluid sample, comprising an insulating substrate having a first recess and a first through hole, a first conductive strips, with the the insulating substrate is connected to the first conductive strips disposed in the first recess, and the first conductive strip having a first output terminal and a second electrical terminal opposite, and at the second terminal is electrically a first test electrode, i.e., the first test electrode is disposed in the first through hole, and a chemical agent, which is disposed on the first test electrode, for detecting a component of the fluid sample, thereby generating a measurement signal, and by the first test electrode to output the measurement signal.

当然,该结构还可以包含一绝缘层,用以使该第一导电条获得一绝缘效果,且该第一测试电极是为该第一导电条的一引出点。 Of course, the structure may further comprise an insulating layer, the first conductive strips for causing an insulating effect is obtained, and that the first test electrode is a lead-out point of the first conductive strip.

较佳者,该结构的第一测试电极是为一工作电极,且该结构还具有一第二导电条,而该绝缘基材即设有一第二凹槽及一第二贯孔,以分别设置该第二导电条及该第二导电条的一第二测试电极。 Are preferred, the first test electrode structure for a working electrode, and the structure further having a second conductive strip, and the second insulating substrate that is provided with a recess and a second through hole, are provided to a second test electrode and said second conductive strips of the second conductive strip. 该第二测试电极可为一对电极(Counter Electrode)。 The second test electrodes may be a pair of electrodes (Counter Electrode).

较佳者,该结构的还具有一第三导电条,而该绝缘基材即设有一第三凹槽及一第三贯孔,以分别设置该第三导电条及该第三导电条的一第三测试电极,该第三测试电极可为一参考电极(Reference Electrode)。 Preferably those, the structure further having a third conductive strip, and the third insulating substrate that is provided with a recess and a third through hole, are provided to the third conductive strip and the conductive strip of a third the third test electrode, the third electrode may be a test reference electrode (reference electrode).

较佳者,该绝缘基材具有一流入凹处,以供该流体样本的流入,而该绝缘基材具有一流体入口,该流体入口、该流入凹处及该第一贯孔为一体成型。 Preferably who flows into the insulating substrate has a recess, for the inflow of the fluid sample, and the insulating substrate having a fluid inlet, the fluid inlet, flows into the first through hole and said recess are integrally formed.

当然,该结构还可以包括一覆盖层,而该绝缘基材的该流入凹处是具有一置放凹处,以供该化学试剂的完整置放,且该覆盖层与该流入凹处形成一 Of course, the structure may further comprise a cover layer, which flows into the recess of the insulating substrate is disposed having a recess, disposed for the complete chemical reagents, and the cover layer and the flow into a recess formed

当然,该结构的覆盖层可以为一塑胶片,该测量区域的一测量底面是与该第一测试电极的一电极顶面形成一高度差,且电极顶面与该置放凹处形成一合成底面,以供该化学试剂的涂布。 Of course, the cover layer of the structure may be a plastic film, a bottom surface of the measured measurement region is formed with a height difference between a top surface of the first electrode of the test electrode, and the electrode top surface of the holder is formed with a recess Synthesis the bottom surface, for applying the chemical agent.

较佳者,该结构的化学试剂是以一定量型式注入该置放凹处,而于该合成底面上形成该化学试剂的一均匀厚度。 It is preferred, configuration of the chemical agent is injected into the amount of pattern placement recess, and the bottom surface of the composite to form a uniform thickness of the chemical agent.

较佳者,该结构的置放凹处具有一凹处厚度,且该凹处厚度即为该化学 It is preferred, the recess is placed a recess structure having a thickness, and the recess is the thickness of the chemical

试剂的该均匀厚度。 The reagent of uniform thickness.

当然,该结构的覆盖层可以具有一印刷导电金属膜(Conductive Metal Film),并以该导电膜作为其一测试电极。 Of course, the cover layer of the structure may have a printed conductive metal film (Conductive Metal Film), and in that one of the conductive film as the test electrode.

当然,该结构的覆盖层是可以为一织布或塑胶网片材质,以呈现一网孔状窗口,且具有一60〜300网目数。 Of course, the cover layer of the structure may be a woven fabric or plastic mesh material, porous net to assume the window, and having a number of 60~300 mesh.

较佳者,该结构的测量区域侧壁与覆盖层内侧是经过一亲水性的覆被处理(Hydrophilic Coating),以利流体被测量区域侧壁完全吸附而不会因空气泡造成测量死角。 Preferably by measuring the inner area of ​​the sidewall and the cover layer of the structure is the result of a hydrophilic coating to be treated (Hydrophilic Coating), the fluid to be measured to facilitate complete adsorption area of ​​the sidewall measured without causing dead due to air bubbles.

较佳者,该结构的测试电极需经过电极的前置处理,如以溶液拋光、以粉体拋光、以纯水或去离子水超声波洗净等各种程序,以确定电极表面干净无吸附其他杂物。 Preferably, tester electrode structure subject to the pre-processing of the electrode, such as the polishing solution to the polishing powder, or a variety of programs to pure water ultrasonic cleaning ions to determine the electrode surface clean and free from other adsorbed debris.

较佳者,该结构的测试电极经过电极的前置处理后再以不同方法与程序, 将该测试电极修饰(Modify)成不同特定用途的修饰电极(Modified Electrode)。 Preferably, testers of the electrode structure and then through priming electrodes in different methods and procedures, and the modified test electrode (the Modify) into a modified electrode of different specific uses (Modified Electrode).

较佳者,将经过电极前置处理后的该结构的第三测试电极以网版印刷方式或电解镀上涂布一氯化银(AgCl)层,经此电极修饰(Modified Electrode) 处理后以形成一氯化银参考电极。 Preferably, it will be tested through the third electrode structure of an electrode after priming screen printing method or an electrolytic plating coated silver chloride (AgCl) layers, after this electrode modified (Modified Electrode) treated to forming a silver chloride reference electrode.

较佳者,该结构的第一测试电极经电极前置处理及电极修饰(Modified Electrode)处理涂布有一层化学试剂(Reagent Matrix),以形成一工作电极。 Are preferred, the first test electrode by the electrode structure and the electrode preprocessing modified (Modified Electrode) coated with a layer of chemical treatment agents (Reagent Matrix), to form a working electrode. 当然,该结构的化学试剂是可以与该流体样本的该成分产生一电化学反应, 并转化该测量信号为一电性参数的测量值,且该电性参数的测量值相关于该成分的一物质浓度。 Of course, the structure of the chemical agent may be produced is a component of the electrochemical reaction with the fluid sample, and the measurement signal is converted to an electrical parameter measurements, and measurements of the electrical parameters associated with a component of the concentration of the substance.

当然,该结构的化学试剂是可以包含酶(Enzyme),酸碱值缓冲剂(PH Buffer)、界面活性剂(Surfactant, Surface Active Agent)、氧化还原反应电子媒介物(Redox Mediator)、亲水性的高分子聚合物(Hydrophilic Ploymer Compound)等组成复合物。 Of course, the chemical agent can comprise an enzyme structure (Enzyme), pH buffer (PH Buffer), surfactant (Surfactant, Surface Active Agent), electron-transfer mediator redox reaction (Redox Mediator), a hydrophilic the polymer (Hydrophilic Ploymer compound) and so complex. 其可以将各化学原料以不同比率分次(或一次) 混合及分次(或一次)将其固化(immobilized、 coated)在工作电极上。 Each of which may be a chemical raw-times at different rates (or primary) are mixed and graded (or primary) curing it (immobilized, coated) on the working electrode.

当然,该结构的化学试剂中的酶可以具有一葡萄糖氧化酶(Glucose Oxidase),以为测试人体的全血(Whole Blood),该流体分析物为人血中的血醣浓度。 Of course, the chemical reagents in the enzyme structure may have a glucose oxidase (Glucose Oxidase), that human whole blood test (Whole Blood), the blood glucose concentration of the analyte in the fluid is human blood. 当然,该结构的化学试剂中的酶可以具有一尿素氧化酶,以为测试人体的全血,该流体分析物为人血中的尿酸浓度。 Of course, the chemical reagents in the enzyme structure may have a urea oxidase, that whole human blood tests, uric acid concentration of the analyte in the fluid is human blood.

当然,该结构的化学试剂是中的酶可以具有一胆固醇氧化酶,以为测试人体的全血,该流体分析物为人血中的胆固醇浓度。 Of course, the structure of the chemical reagents is an enzyme may have a cholesterol oxidase, human whole blood that test, the cholesterol concentration of the analyte fluid is human blood.

当然,该结构的绝缘基材可以具有一第一入口侧及相对一第二气孔侧, 是分别作为该流体入口及形成一气孔开口,且该气孔开口以利于该流体样本作一毛细管流动。 Of course, the insulating base structure may have a first inlet-side and an opposite second side hole, respectively, and a fluid inlet opening formed in a hole, and the hole to facilitate opening of the fluid sample for a capillary flow.

较佳者,该结构的绝缘基材是具有一抵顶凸块,以抵顶该覆盖层,并利用该抵顶凸块来隔离该流体样本与该覆盖层的一黏胶。 Are preferred, the insulating base is a structure having a projection abutting a glue, so as to abut against the covering layer, and by using the abutting projection to isolate the fluid sample and the cover layer.

当然,该结构的绝缘层是可以为一绝缘塑胶片或印刷绝缘胶,其设置于该绝缘基材的一底面,且该第一凹槽亦位于该底面上,而该绝缘基材更具有第一印刷导电金属膜,位于该绝缘基材的一顶面,以为一第二测试电极。 Of course, this structure is an insulating layer may be an insulating plastic film or printing an insulating adhesive, provided in a bottom surface of the insulating substrate, and the first groove is also located on the bottom surface of the insulating substrate and having a first more printing a conductive metal film located in a top surface of the insulation material, that a second test electrode.

当然,该结构的该绝缘基材还具有第二印刷导电金属膜,与该第一印刷导电金属膜同时印刷于该绝缘基材的一顶面,以为一第三测试电极。 Of course, the insulating substrate of the structure further having a second printing conductive metal film, the first conductive metal film printed simultaneously printed on a top surface of the insulation material, that a third test electrode. 并于此电极上设有一氯化银层以修饰该第三电极为一氯化银参考电极。 And an upper electrode provided thereto a silver chloride layer and the third electrode to modify a silver chloride reference electrode.

当然,该结构的第一贯孔及该第一测试电极可以各具有一贯孔截面积及一电极截面积,该贯孔截面积即同于该电极截面积,且该第一贯孔是位于该绝缘基材的一底面。 Of course, the first through hole and the first test electrode structure may each have a through hole and an electrode sectional area of ​​the cross-sectional area, i.e. the cross sectional area of ​​the through hole in the same cross-sectional area of ​​the electrode, and the first through hole is located a bottom surface of the insulating substrate.

较佳者,该结构的绝缘基材是采用一聚氯乙烯PVC(polyvinyl chloride)、 聚丙烯PP (Polypropylene)、聚碳酸酯PC (Polycarbonate)、聚对苯二甲酸亚丁烯基酯PBT (Polybutylene Terephthalate)、聚对苯二甲酸乙二醇酯PET (Polyethylene Terephthalate)、聚氧化亚苯PPO (Modified Polyphenlene Oxide) 或丙烯腈丁二烯苯乙烯树酯ABS (Acrylonitrile Butadiene Stytene)等塑胶树脂。 Are preferred, the insulating substrate of the structure is the use of a polyvinyl chloride PVC (polyvinyl chloride), polypropylene PP (Polypropylene), polycarbonate PC (Polycarbonate), poly (Polybutylene Terephthalate Terephthalic acid ester PBT butenylene ), polyethylene terephthalate PET (polyethylene terephthalate), poly phenylene oxide PPO (Modified Polyphenlene oxide) or acrylonitrile butadiene styrene resin ABS (acrylonitrile butadiene Stytene) and other plastic resins.

较佳者,该结构的第一导电条是为一先行成型,而于一绝缘基材成型装置中埋入该第一导电条,以形成该绝缘基材。 It is preferred, the first conductive strips is a first structure formed, and the first conductive strips embedded in the insulating base in a molding apparatus to form the insulating substrate.

当然,该结构的第一导电条与该绝缘基材是可以一双材料射出方式一次成型。 Of course, the first conductive strip and the insulating substrate of the structure is one pair of molding material emitted manner.

当然,该结构的第一导电条是可以利用一黏胶以接合至该绝缘基材上。 Of course, the structure of the first conductive strips is to use a glue bonded onto the insulating substrate.

又依照本发明另一方面, 一种电化学式传感器的结构,是用于检测一流体样本,其包含一绝缘基材,其具有一第一凹陷空间, 一第一导电装置,容 In accordance with yet another aspect of the present invention, the structure of an electrochemical sensor, for detecting a fluid sample, comprising an insulating substrate having a first concave space, a first electrically conductive means receiving

设于该第一凹陷空间,该第一导电装置具一第一输出端及相对一第二电极端, 且以该第二电极端为一第一测试电极,以及一化学试剂,其设置于该第一测试电极上,用以检测该流体样本的一成份,进而产生一测量信号,并经该第一测试电极以输出该测量信号。 The recess provided in the first space, the first conductive means having a first output terminal and a second electrical terminal opposite, and to the second electrical terminal is a first test electrode, and a chemical agent, which is disposed in the a first test electrode, for detecting a component of the fluid sample, thereby generating a measuring signal, via the first test electrode and to output the measurement signal.

较佳者,该结构的第一导电装置是为一第一导电条,且该第一凹陷空间包括有一第一凹槽及一第一贯孔,并于该第一凹槽内设置该第一导电条,该第一测试电极即设置于该第一贯孔中。 Are preferred, the configuration of the first conducting means is a first conductive strip and the first recess comprises a first recess space and a first through hole, and disposed within the first recess of the first conductive strip, i.e. the first test electrode is disposed in the first through hole.

较佳者,该结构的该第一导电装置是为一第一导电垫块(Pad),导电垫块(Pad)的第一面,具有测试电极的工作面积,且该绝缘基材上的第一凹陷空间为一第一贯孔,该结构还具有一金属导电膜(Conductive Metal Film)以为该电极的导引电线(Lead),其被印刷于一绝缘基材上,该印刷导电金属膜的一侧并覆盖连接至该第一导电垫块的第二面相衔接(电极工作面积的背面), 金属导电膜的另一侧以为该测量信号的第一输出端。 Preferably by the first conductive means of the structure is the work area of ​​a first conductive pad (Pad), a first surface of the conductive pad (Pad), with a test electrode, and the second on the insulating substrate a recessed space for a first through hole, the structure further having a conductive metal film (conductive metal film) that guide the wire electrode (Lead), which is printed on an insulating substrate, the printed conductive metal film and covering a second side surface of the first conductive pad connected to the adapter (work area of ​​the back surface electrode), the other side of the metal conductive film that a first output terminal of the measurement signal. 此用传统印刷方法以低价金属导电膜(如银胶印刷导电膜)方法来代替Lead段,以降低电极的Lead 段的阻抗,此金属导电膜已远离电极工作面积不会与待测流体接触而无化学干扰问题,且其与电极工作面积无直接相接,故其虽有印刷成形的尺寸不稳定,但其却完全不会影响塑胶射出导电电极工作面积尺寸大小的稳定性。 This conventional method of printing at a low price metal conductive film (conductive film such as silver paste printing) method instead Lead section, so that the impedance of the electrode segment Lead This metal conductive film is not in contact with the fluid to be measured from the electrode working surface without chemical interference problems, and it has no direct contact with the working electrode area, so the printing dimensionally unstable although shaped, but it will not affect the stability of the plastic injection conductive electrode size of the working area.

本发明若以含碳塑胶导电材料射出成形该导电条的电极的总阻抗,虽比用碳膜印刷所成形的电极的电极阻抗低了数十倍,但其仍比用金属膏的印刷导电膜(如银胶)的导电阻抗高。 In terms of the carbonaceous conductive material emits plastic total impedance of the electrodes of the conductive strip forming the present invention, although the number of times the low impedance electrode formed by printing carbon electrodes, but still higher than the conductive film by printing a metal paste ( the silver paste) conductive high impedance. 因银胶有化学干扰问题不适合于工作电极使用,所以用本发明以含碳塑胶导电材料射出成形的工作电极能获得无化学干扰、稳定的工作电极尺寸与低的电极电阻(相对于无化学干扰的碳膜印刷电极)的优点。 Because of chemical interference silver paste is not suitable for use in the working electrode, the present invention is to use the carbon-containing conductive material plastic working electrode Injection molding can be obtained without chemical interference, the size of the working electrode and the stable low electrode resistance (relative to no chemical advantage carbon printed electrodes) interference. 但由于参考电极(Reference Electrode)与对电极(Counter Electrode)与各电极的引导线(Lead)皆无化学干扰与电极尺寸稳定的需求问题,因而可使用低价的金属膏印刷导电膜(如银胶)以获取更低的导电阻使传感器的测量更稳定。 However, since the reference electrode (Reference Electrode) and the counter electrode (Counter Electrode) guidewire with each electrode (Lead) without chemical interference and are dimensionally stable electrode demand, allowing the use of inexpensive metal film printed conductive paste (such as silver glue) to obtain a lower-resistance of the measuring sensor is more stable. 此混合塑胶射出导电条电极与传统印刷金属膏导电膜的整合,可得相辅相成的效果,而其与本专利精神欲以低成本实现无化学干 This integration of mixed plastic injection bar electrode and the metal conductive film traditional printing paste, synergistic effect can be obtained, while the spirit of the present patent trying to use a low cost without chemical dry

扰的工作电极、低的电极总阻抗及稳定的电极工作面积等目的完全相同。 Disturbance of the working electrode, the low impedance of the electrode and the total area of ​​the working electrode other stabilization purposes identical.

当然,该结构的第一贯孔更可以具有一扩大凹槽,以置放该第一导电垫块的一扩大底座。 Of course, the structure of the first through hole may have a further enlarged recess disposed in the base a first conductive pad to expand.

再按照另一种相当的技术层面来看,本发明的一种电化学式传感器的结构,是用于检测一流体样本,其包含一第一导电元件,其具有一第一测试电极, 一绝缘基材,是具有一第一凹陷空间,以置放该第一导电元件, 一第一印刷导电金属膜,其与该绝缘基材相接,该第一印刷导电金属膜具一第一输出端及相对一第二衔接端,且以该第二衔接端与该第一导电元件相衔接,以及一化学试剂,其设置于该第一测试电极上,用以检测该流体样本的一成份, 进而产生一测量信号,并经该第一测试电极以输出该测量信号。 According to another further considerable technical perspective, the structure of an electrochemical sensor according to the present invention, for detecting a fluid sample, comprising a first conductive element having a first test electrode, an insulating base material having a first recessed space disposed to the first conductive member, a first printing conductive metal film, which is in contact with the insulating substrate, the first printing conductive metal film having a first output terminal and opposing a second adapter end and to the second terminal of the adapter engaged with the first conductive element, and a chemical agent, which is disposed on the first test electrode, for detecting a component of the fluid sample, thereby generating a measurement signal, via the first test electrode and to output the measurement signal.

当然,该结构的绝缘基材是可以具有一第二凹陷空间,以置放一第二导电元件,且该结构更具有一第二印刷导电金属膜,其与该第一印刷导电金属膜同时被印刷于该绝缘基材上,用以衔接该第二导电元件。 Of course, the insulating base structure may have a second recess is a space for placing a second conductive member, and the structure further having a second printing conductive metal film, simultaneously with the first printing conductive metal film is printed on the insulating substrate, for engaging the second conductive element.

较佳者,该结构第一凹陷空间还与一侧部凹槽相连以形成一"U"形凹槽, Preferably person, the spatial structure of the first recess and the further recess side portions joined to form a "U" shaped groove,

以容纳一"u"形导电元件,且该结构还包括一绝缘层,是用以覆盖该第一印刷 Shaped conductive member to receive a "u", and the structure further comprises an insulating layer, covering the first printing is used

导电金属膜,该绝缘层并具有一流入凹处,以供该流体样本的流入,且该绝缘层具有一概略呈"匸"形开口,以形成一测量空间。 Conductive metal film, the insulating layer and having an inflow recess, for the inflow of the fluid sample, and the insulating layer has not and will slightly "Xi" shaped opening to form a measuring space.

较佳者,该结构的还包括有一第三印刷导电金属膜,是与该第一印刷导电金属膜同时被印刷于该绝缘基材上。 Preferably those, the structure further comprises a third printed conductive metal film is first printed with the conductive metal film is simultaneously printed on the insulating substrate.

如从另一种对等的技术层面来看,本发明的一种电化学式传感器的结构, As another peer from a technical perspective, an electrochemical sensor structure of the present invention,

用于检测一流体样本,其包含: 一导电片,其具有一第一输出端及一第二电极端,且以该第二电极端为一第一测试电极; 一绝缘基材,是与该导电片相接,且具有一电极贯孔,以置放该第一测试电极;以及一化学试剂,其设置于该第一测试电极上,用以检测该流体样本的一成份,进而产生一测量信号, 并经该第一测试电极以输出该测量信号。 For detecting a fluid sample, comprising: a conductive sheet having a first output terminal and a second electrical terminal, and to the second electrical terminal is a first test electrode; an insulating substrate, with the a conductive contact plate and having a through hole electrode, disposed to the first test electrode; and a chemical agent, which is disposed on the first test electrode, for detecting a component of the fluid sample, thereby generating a measurement signal, and by the first test electrode to output the measurement signal.

当然,该结构的第一测试电极是可以位于该导电片的一凸出部位上,以置入该绝缘基材的该电极贯孔。 Of course, the first test electrode structure is located on a projecting portion of the conductive sheet to an electrode placed in the through hole of the insulation material.

当然,该结构还可以包括一修饰电极,设置于一第二印刷导电金属膜上, 而成为一参考电极。 Of course, the structure may further comprise a modified electrode disposed on a second printed conductive metal film, becomes a reference electrode.

本发明经由上述构想的解说,即能观察到所运用的电化学式传感器的制造方法及其结构,确实能置放一绝缘基材到射出成型装置内,具有形成第一 Contemplated by the present invention illustrated above, i.e., it can be observed the production method and structure of the electrochemical sensor by the use of an insulating substrate really disposed to exit the molding apparatus, having a first forming

导电条(Conductive Strip)于该绝缘基材的一第一凹槽内的特色。 Conductive strips (Conductive Strip) features a first recess in the insulating base material.

为了易于说明,本发明得藉由下述的较佳实施例及图示而得到一更加了解。 For ease of illustration, the present invention is obtained by the illustrated preferred embodiment and the following embodiments give a better understanding.

(4)附图说明 (4) Brief Description of Drawings

图1是先前技术的电化学检测装置的立体示意图; FIG 1 is a perspective schematic view of the previous art of electrochemical detection means;

图2是另一先前技术的拋弃式印刷电极的平面示意图; FIG 2 is another plane prior art disposable schematic printed electrode;

图3是图2的拋弃式印刷电极的立体分解示意图; FIG 3 is a perspective view of the disposable electrode printing exploded view of FIG. 2;

图4是本发明的电化学式传感器单电极的制造方法及其结构的较佳实施例的立体爆开示意图; FIG 4 is a perspective schematic view of the preferred embodiment of the burst of the manufacturing method and structure of a single electrode electrochemical sensor of the present invention;

图5是图4的传感器单电极的反面立体爆开示意图; 图6是图4的传感器单电极与一化学试剂结合前的立体示意图; 图7是利用图4的传感器的电化学检测装置的立体示意图; 图8(a)〜(b)是本发明的又一较佳实施例的二极式电化学传感器的立体正面组合及爆开示意图; FIG 5 is a back perspective view of a single electrode sensor of FIG. 4 schematic burst open; FIG. 6 is a perspective view of the front view of the sensor 4 with a single electrode in conjunction with a chemical agent; FIG. 7 is a perspective view of an electrochemical detection apparatus using the sensor of FIG. 4 a schematic diagram; FIG. 8 (a) ~ (b) is a perspective front two-electrode electrochemical sensor combination according to still another preferred embodiment of the present invention and a schematic burst;

图9(a)是图8(b)的传感器的正面局部分解的立体示意图;图9(b)是图8(b) 传感器的电极的导电截面机与导体厚度的立体示意图; Perspective schematic view 9 (a) is a view 8 (b) is a partially exploded front of the sensor; FIG. 9 (b) is a perspective view of a conductor conducting a cross-sectional thickness of the electrode unit in FIG. 8 (b) of the sensor;

图10是本发明的又一较佳实施例的三极式电化学传感器的立体正面爆开示 FIG 10 is a perspective front three-electrode electrochemical sensor according to still another preferred embodiment of the present invention illustrating a burst

意图; intention;

图ll(a广(c)是本发明的再一较佳实施例的四极式电化学传感器的立体正面、立体反面及正面爆开示意图; FIG. Ll (a wide (c) is a front perspective view of the present invention, a further four-pole electrochemical sensor of the preferred embodiment, the front and back perspective schematic burst;

图12 (a)是本发明的又一较佳实施例的三极式电化学传感器的立体正面爆开示意图; FIG. 12 (a) is a perspective front three-electrode electrochemical sensor according to still another preferred embodiment of the present invention, a schematic diagram of the burst;

图12(b)是图12(a)的电化学传感器被置于一承装容器内的立体剖面示意图; 图13(a)是本发明的再一较佳实施例的三极式电化学传感器的立体正面爆开示意图; FIG. 12 (b) is a diagram 12 (a) an electrochemical sensor is placed in a cross-sectional schematic perspective holding container; FIGS. 13 (a) is a three-electrode electrochemical sensor of the present invention, a further preferred embodiment a front perspective schematic burst;

图13(b)是图13(a)的正面组立示意图。 FIG. 13 (b) is a front view 13 (a) is a schematic vertical group.

图14(a)是本发明的又一较佳实施例的两极式电化学传感器的立体正面爆开示意图。 FIG 14 (a) is a front perspective bipolar electrochemical sensor according to still another preferred embodiment of the present invention, a schematic burst open.

图14(b)是本发明的又一较佳实施例的三极式电化学传感器的立体正面爆开 FIG. 14 (b) is a front perspective burst open three-electrode electrochemical sensor according to still another preferred embodiment of the invention

示意图。 FIG.

图15是一本发明的电化学传感器试片插入至一习知电化学计量器的的立体组合示意图。 FIG 15 is an electrochemical sensor of the present invention is a test strip is inserted into a conventional perspective assembled view of an electrochemical meter.

图16是本发明的又一较佳实施例的单极式电化学传感器的立体反面爆开示意图。 16 is a schematic burst monopolar electrochemical sensor according to still another preferred embodiment of the present invention, the opposite perspective.

图17是图16的电化学传感器的立体正面爆开示意图; FIG 17 is a front perspective view of the electrochemical sensor 16 of FIG schematic burst;

图18是本发明的再一较佳实施例的二极式电化学传感器的立体反面爆开示意图;以及 FIG 18 is a schematic perspective back burst open again the present invention is a two-pole electrochemical sensor according to the preferred embodiment; and

图19(a)〜(d)是本发明的再一较佳实施例的三极式电化学传感器的立体反面 FIG 19 (a) ~ (d) of the present invention, then a reverse perspective three-electrode electrochemical sensor according to the preferred embodiment

组合、正面组合、正面局部分解及反面爆开的示意图; Combination, a combination of positive, negative partial schematic front exploded and burst open;

图20(a), (b)是本发明的又一较佳实施例的三极式电化学传感器的立体正面爆开及组合示意图;以及 FIG 20 (a), (b) is a perspective assembly view of a front burst and a three-electrode electrochemical sensor according to still another preferred embodiment of the present invention; and

图20(c)是本发明的再一较佳实施例的三极式电化学传感器的立体正面爆开示意图。 FIG. 20 (c), then the present invention, preferred is a perspective front three-electrode electrochemical sensor embodiments of the burst Fig.

(5)具体实施方式 (5) DETAILED DESCRIPTION

请参阅图4至6,显示出一种电化学式传感器的制造方法,包含:提供一 See FIGS. 4-6, showing a method of manufacturing an electrochemical sensor, comprising: providing a

射出成型装置(模具,图中未示出),提供一绝缘基材40,其具有一个沿绝缘基材的长度方向延伸的第一凹槽50 (详见图5)及一个位于绝缘基材一端上的第一贯孔41,将绝缘基材40送入该射出成型装置内(模具),注入一塑胶导电的成型材料至该射出成型装置内,以于第一凹槽50内形成一第一导电条(Conductive Strip) 42,而第一导电条42具有一第一输出端43及一导引线lead 45及相对的一第二电极端44,且以第二电极端44为一第一测试电极44,第一测试电极44即于第一贯孔41中形成,以及提供如图6所示的一化学试剂60,其设置于第一测试电极44上,用以检测一流体样本,化学试剂60即与该流体样本的一成分反应,进而产生一测量信号,并经第一测试电极44以输出该测量信号。 Injection molding apparatus (mold, not shown in FIG), providing an insulating substrate 40, a first groove having a longitudinal direction extending along the insulating substrate 50 (see FIG. 5), and one end of the insulating substrate first through hole 41, the insulating substrate 40 into the inner injection molding apparatus (mold), injecting a plastics molding material to the conductive inner injection molding apparatus to form a first recess 50 in the first conductive strips (conductive strip) 42, and the first conductive strip 42 having a first test a first output terminal 43 and a guide wire and a second lead 45 electrically opposite terminal 44, and the second terminal 44 is electrically electrode 44, i.e., the first test electrode 44 is formed in the first through hole 41, and providing a chemical agent 60 as shown in FIG. 6, which is disposed on the first test electrode 44 for detecting a fluid sample, Chemicals 60 with a component that is the reaction of the fluid sample, thereby generating a measuring signal, via the first test electrode 44 and to output the measurement signal.

该方法的第一测试电极44输出该测量信号至一计量器,且该塑胶导电材料是由一导电碳材或一金属粉与一树酯基材所合成,该树脂基材为一热成形 The first electrode of the test method 44 outputs the measurement signal to a meter, and the conductive plastic material is synthesized by a carbon material or a conductive metal powder and a resin substrate, the resin substrate is a thermoformed

的塑胶树酯'(plastic resin)。 Plastic resin '(plastic resin). 而该塑胶导电材料的该导电碳材可以为一碳黑(Carbon Black)、石墨(Graphite Powder)、碳纤维(Carbon Fiber)或纳米碳管(Carbon Nanotube),至于该金属粉则可以为钯、铑、白金、金等。 The electrically conductive carbon material and the plastic material may be a conductive carbon black (Carbon Black), graphite (Graphite Powder), carbon fiber (Carbon Fiber) or carbon nanotube (Carbon Nanotube), as for the metal powder that can be palladium, rhodium , platinum and gold. 且使用该导电碳材时,可具有该塑胶基材的一3〜60%的重量比。 And when the conductive carbon material used, the plastic substrate may have a ratio of 3~60 wt%. 使用该金属粉时, 则可具有该塑胶基材的一0. 1〜5%的重量比,并可导电碳与金属粉任何两种以上混合使用。 Of a 0. 1 ~ 5% by weight of the metal powder used may be compared with the plastic substrate, and the metal powder mixed with a conductive carbon used any two or more thereof. 该塑胶导电材料的该树脂基材可以为一环氧树酯(印oxy),其与该导电碳材或该金属粉搅拌呈一液态而注入绝缘基材40的第一凹槽50及第一贯孔41,第一导电条42即与绝缘基材40形成一紧密配接。 The resin base material of the plastic material may be a conductive epoxy resin (printing oxy), with the conductive carbon material of the metal powder or a liquid injected was stirred first recess 40 of the insulating substrate 50 and the first through hole 41, i.e., the first conductive strip 42 is formed with a close contact with the insulating substrate 40.

本发明的一种电化学式传感器,用于检测一流体样本,其包含一绝缘基材40,其具有一第一凹槽50及一第一贯孔41, 一第一导电条42,是与绝缘基材40相连接,以将第一导电条42设置于第一凹槽50内,而第一导电条42 具有一第一输出端43及一导引线lead 45及相对一第二电极端44,且以第二电极端44为一第一测试电极44,第一测试电极44即设置于第一贯孔41中, 以及一化学试剂60,其设置于第一测试电极44上,用以检测该流体样本的一成份,进而产生一测量信号,并经第一测试电极44以输出该测量信号。 An electrochemical sensor according to the present invention, for detecting a fluid sample, which comprises an insulating substrate 40, a first groove having a first through hole 50 and 41, a first conductive strips 42, with an insulating substrate 40 is connected to the first conductive strips 42 disposed in the first recess 50, and the first conductive strip 42 having a first output terminal 43 and a lead 45 and the guidewire relative to a second electric terminal 44 and a second electrical terminal 44 as a first test electrode 44, i.e., the first test electrode 44 disposed in the first through hole 41, and a chemical agent 60, which is disposed on the electrode 44 to the first test for detecting a component of the fluid sample, thereby generating a measuring signal, via the first test electrode 44 and to output the measurement signal.

请参阅图8(b),本发明的结构还可以包含一绝缘层80,用以使第一导电条81获得绝缘效果,且第一测试电极82是为第一导电条81的一电极引出点82。 See FIG. 8 (b), the structure of the present invention may further comprise an insulating layer 80, first conductive strips 81 for causing an insulating effect is obtained, and the first test electrode 82 is a first conductive strips of the electrode lead-out point 81 82. 该结构的第一测试电极82是为一工作电极82,且还具有一第二导电条83, 而绝缘基材84即设有一第二凹槽85及一第二贯孔86,以分别设置第二导电条83及第二导电条83的一第二测试电极87。 The first test electrode structure 82 is a working electrode 82, and further having a second conductive strips 83, and the insulating substrate 84 that is provided with a second recess 85 and a second through hole 86, respectively, to set the first two conductive strips 83 and the second conductive strips 83, a second test electrode 87. 第二测试电极87为一对电极87。 The second test electrode 87 as a pair of electrodes 87.

请参阅图8(b),该绝缘基材84具有一流入凹处88,以供该流体样本的流入,而绝缘基材84具有一流体入口89,流体入口89、流入凹处88、第一贯孔891及第二贯孔86为一体成型。 See FIG. 8 (b), the insulating substrate 84 having a recess 88 flows to the fluid sample for the inflow, and the insulating substrate 84 having a fluid inlet 89, fluid inlet 89, flows into the recess 88, a first a second through hole 891 and the through hole 86 is integrally formed. 该结构还可以包括一覆盖层892,而绝缘基材84的流入凹处88具有一置放凹处894,以供化学试剂895的完整置放, 且覆盖层892与流入凹处88形成一测量区域893。 The structure may further include a measurement of a cover layer 892, and flows into the recess 84 of the insulating substrate 88 having a recess 894 disposed, for placing full chemical agent 895, and the cover layer 88 is formed a recess 892 with the inflow 893 area. 覆盖层892可以为一透明或半透明塑胶片,该测量区域的一测量底面90 (详见图9(a))是与第一测试电极82的一电极顶面91形成一高度差A ,且电极顶面91与置放凹处894形成一合成底面92,以供化学试剂895的涂布。 Capping layer 892 may be a transparent or translucent plastic sheet, a bottom surface measuring 90 (see FIG. 9 (a)) of the measurement region A is formed a height difference between the first test electrode and a top surface 82 of the electrode 91, and electrode 91 and the top surface of the recess 894 is placed the bottom surface 92 form a composite, for applying a chemical agent 895. 化学试剂895是调成液态后以一定量型式注入置放凹处894,而于合成底面92上形成化学试剂895的一均匀厚度A 。 Chemicals 895 after a certain amount of liquid transferred into injection pattern disposed recess 894 to form a uniform thickness A chemical agent 895 in the bottom surface 92 synthesized.

请参阅图8(b),图中显示出一覆盖层892上有一透明窗口8% (或半透明窗口亦可),以利一使用者观察该流体样本,而透明窗口896印有一方向记号898,以提醒该使用者,绝缘基材84具有二抵顶凸块897,以抵顶覆盖层892,并利用抵顶凸块897来隔离该流体样本与覆盖层892的一黏胶。 See FIG. 8 (b), there is a graph showing a cover layer 892 on a transparent window 8% (or translucent window versa) to facilitate a user to observe the fluid sample, the transparent window 896 and a direction mark printed 898 to remind the user, the insulating substrate 84 has two abutting projections 897, so as to abut against the covering layer 892 and the bump 897 using abutting to isolate the fluid sample with a covering layer of glue 892.

请参阅图9(a),该绝缘基材可以具有一第一入口侧93及相对的一第二气孔侧94,是分别作为流体入口89及形成一气孔开口94,且气孔开口94以利于该流体样本作一毛细管流动。 See FIG. 9 (a), the insulating substrate may have a first inlet-side 93 and a second opposite side of the hole 94, respectively, is formed as a fluid inlet 89 and an air vent opening 94, 94 to facilitate opening of the stomata a fluid sample for capillary flow.

请参阅图10,其结构的还具有一第三导电条100,而绝缘基材101设有一第三凹槽102及一第三贯孔103,以分别设置第三导电条100及第三导电条100的一第三测试电极104,第三测试电极104即为一参考电极(Reference Electrode)。 Please refer to FIG. 10, the structure further having a third conductive strip 100, and the insulating substrate 101 is provided with a third groove 102, and a third through hole 103, and 100 are provided to the third conductive third conductive strips a third test 100 of electrode 104, the third electrode 104 that is a test reference electrode (reference electrode).

请参阅图10,该结构的覆盖层107是可以为一织布或塑胶网片材质,以呈现一网孔状窗口109,且具有60~300网目数。 Please refer to FIG. 10, the structure of the cover layer 107 may be a woven fabric or plastic mesh material, to present a net-shaped window hole 109, and has a number of 60 to 300 mesh. 而在一测量凹面108与该覆盖层的底面1091是经过亲水性的覆被处理(hydrophilic coating)。 In measuring the concave surface 108 and a bottom surface of the cover layer 1091 is the result of coating the treated hydrophilic (hydrophilic coating).

请参阅图6、图7、图8(b)、图10、图11、图12(a)及图13,该结构的第一测试电极(工作电极)或第二测试电极(对电极)或第三测试电极(参考电极)可经过电极的前置处理,如以溶液拋光、以粉体拋光、以纯水或去离子水超声波洗净等各种程序,以确定电极表面干净无吸附其他杂物。 Refer to FIG. 6, FIG. 7, FIG. 8 (b), FIG. 10, FIG. 11, FIG. 12 (a) and FIG. 13, the first test electrode (working electrode) of the structure or the second test electrode (counter electrode) or the third test electrodes (reference electrode) may be subjected to pre-processing of the electrode, such as the polishing solution to the polishing powder, or a variety of programs to pure water ultrasonic cleaning ions to determine the electrode surface clean and free from adsorbed miscellaneous thereof.

请参阅图6、图7、图8(a)、图8(b)及图10、图11、图12(a)及图13, 该结构的第一测试电极(工作电极)或第三测试电极(参考电极)经过电极的前置处理后,再以不同方法与程序,将该测试电极修饰(Modify)成不同特定用途的修饰电极(Modified Electrode)。 Refer to FIG. 6, FIG. 7, FIG. 8 (a), FIG. 8 (b) and FIG. 10, FIG. 11, FIG. 12 (a) and FIG. 13, the first test electrode (working electrode) of the structure or the third test electrode (reference electrode) after pre-treatment electrode, and then to different methods and procedures, and the modified test electrode (the modify) into a modified electrode of different specific uses (modified electrode). 如将经过电极前置处理后的第三测试电极以网版印刷方式或电解镀上涂布一氯化银(AgCl)层,如图10 的105、图12(a)和图12(b)的126、图13的138及图7中可见第三测试电极71是设置有一银/氯化银层72,经此电极修饰(Modified Electrode)处理 As will be tested through the third electrode after the electrode is pre-treated by screen printing method or an electrolytic plating coated silver chloride (AgCl) layer as the 10510, FIG. 12 (a) and 12 (b) 126, 138 in FIG. 13 and FIG. 7 seen third test electrode 71 is provided with a silver / silver chloride layer 72, this electrode was modified (modified electrode) processing

后以形成一氯化银参考电极,而标号为73的为一对电极。 After to form a silver chloride reference electrode, and reference numeral 73 is a pair of electrodes.

而该结构的第一测试电极经电极前置处理及电极修饰(Modified Electrode)处理涂布有一层化学试剂(Reagent Matrix),以形成一工作电极,如在图7的工作电极44,图8(b)的化学试剂895、图10的化学试剂106 及图12(a)和图12(b)的124等。 The test electrode and the first electrode structure by electrodes pre-processing and modification (Modified Electrode) coated with a layer of chemical treatment agents (Reagent Matrix), to form a working electrode as a working electrode 44 in FIG. 7, FIG. 8 ( b) chemical reagents 895, 106 and 124 and other chemical reagents 10 of FIG. 12 (a) and 12 (b) is. 该化学试剂是可以与该流体样本的该成分产生一电化学反应,并转化该测量信号为一电性参数的测量值,且该电性参数的测量值相关于该成分的一物质浓度。 The chemical agent may be produced is a component of the electrochemical reaction with the fluid sample, and the measurement signal is converted to an electrical parameter measurements, and measurements of the electrical parameters related to the concentration of a substance in a composition. 该修饰工作电极的化学试剂可以包含酶(Enzyme如Glucose Oxidase, Uricase等酶催化剂),酸碱值缓冲剂(PH Buffer如Citrate buffer)、界面活性齐廿(Surfactant, Surface Active Agent 如3M的FC-170C)、氧化还原反应电子媒介物(Redox Mediator如Ferricyanide)、亲7K性的高分子聚合物(Hydrophilic Ployroer Compound, 如Polyethylene Oxide或Carboxymethyl Cellulose等的混合)等组成复合物。 The modified working electrode may contain the enzyme chemical agent (Enzyme such Glucose Oxidase, Uricase enzyme catalyst, etc.), pH buffer (PH Buffer such Citrate buffer), a surfactant Qi twenty (Surfactant, Surface Active Agent, such as 3M's FC- 170C), electron-transfer mediator redox reaction (redox Mediator such ferricyanide), pro-7K of polymer (Hydrophilic Ployroer compound, such as Polyethylene oxide or the like mixed Carboxymethyl Cellulose) and so complex. 其可以将各化学原料以不同比率分次(或一次)混合及分次(或一次) 将其固化(immobilized、 coated)在工作电极上。 Each of which may be a chemical raw-times at different rates (or primary) are mixed and graded (or primary) curing it (immobilized, coated) on the working electrode.

该结构的化学试剂中的酶可以具有一葡萄糖氧化酶(Glucose Oxidase), 以为测试人体的全血(Whole Blood),该流体分析物为人血中的血醣(Glucose) 浓度。 Chemicals in the structure may have an enzyme glucose oxidase (Glucose Oxidase), that human whole blood test (Whole Blood), the fluid is human blood analyte glucose (Glucose) concentration.

该结构的化学试剂中的酶可以具有一尿素氧化酶(Uricase),以为测试人体的全血,该流体分析物为人血中的尿酸(Uric Acid)浓度。 Chemicals in the structure may have a urea enzyme oxidase (uricase), human whole blood that test, the fluid in the analyte is human serum uric acid (Uric Acid) concentration.

该结构的化学试剂是中的酶可以具有一胆固醇氧化酶(Cholesterol Esterase and Cholesterol Oxidase),以为测试人体的全血,该流体分析物为人血中的胆固醇(Cholesterol)浓度。 The chemical agent is an enzyme structure may have a cholesterol oxidase (Cholesterol Esterase and Cholesterol Oxidase), human whole blood that test, the fluid analyte human blood cholesterol (Cholesterol) concentration.

请参阅图11,覆盖层110是可以经由印刷而具有一印刷导电金属膜111, 导电金属膜111可印刷成两隔离导电膜以为一参考电极112与一对电极114, 并与绝缘基材116相接, 一气孔118则与一流量检测电极115共同位于第二贯孔117内,此对流气孔118、流入凹处1193与流体入口1192形成流体的毛细对流通道。 Please refer to FIG. 11, the cover layer 110 can be printed via a metal having a printed conductive film 111, a conductive metal film 111 into two isolated conductive film is printed that a reference electrode 112 and a pair of electrodes 114, 116 and the insulating substrate then, a hole 118 with a flow rate detector common electrode 115 located in the second through hole 117, the convection hole 118, flows into the recesses 11,931,192 fluid inlet and a fluid convection channels. 1121、 1131、 1141、 1151为各测试电极的输出端,另一"匸〃 型开口1191以为印刷导电金属膜电极输出端1121、 1141的位置。该第四电极115为一流体的流量检测电极,当流体由流体入口1192经流入凹处1193、 1121, 1131, 1141, 1151 is the output end of each test electrode and the other "Xi 〃 shaped openings 1191 that the printed conductive metal film electrode output terminal 1121, 1141 of the position. The fourth electrode 115 is a fluid flow rate sensing electrode, when the fluid flows from the fluid inlet 1192 through pocket 1193,

工作电极113最后流入至第四电极115始完成充填,若流体未到达第四电极 And finally into the working electrode 113 to the beginning of the fourth electrode 115 to complete the filling, if the fluid does not reach the fourth electrode

115,则计量器(参阅图15的计量器150)可检知第四电极115未与其他电极经流体相通而得知流体未填满。 115, the meter (see FIG. 15 150 meter) detecting the fourth electrode 115 can not be learned through the fluid communication with the fluid does not fill the other electrode.

请参阅图12(a),该结构的绝缘基材120的背面还印刷上一层绝缘胶层121,以对导电条除电极面积及电极输出端以外的部分形成一绝缘保护作用, 以利整个传感器可直接浸入待测溶液中。 See FIG. 12 (a), the back surface of the insulating base material of the structure 120 further subbing layer printed on the insulating layer 121 to portions of the conductive strip and the electrode other than the electrode area of ​​the output terminal forming an insulating protective effect, in order to facilitate the entire sensors can be directly immersed in the test solution. 图12(b)可见在一承装容器127内的工作电极123上的化学药剂124,参考电极125上的氯化银修饰电极126,而对电极在122的位置,整个传感器可直接浸入待测溶液中。 FIG. 12 (b) chemicals visible on the working electrode 123 within a holding container 127 124, silver chloride reference electrode 125 on the modified electrode 126, and the position of the pair of electrodes 122, the entire sensor can be directly immersed in the test solution. 该化学试剂可以与该流体样本的该成分产生一电化学反应,并转化该测量信号为一电性参数的测量值,且该电性参数的测量值相关于该成分的一物质浓度。 The chemical agent may be produced with the components of the fluid sample to an electrochemical reaction, and conversion of the measurement signal is a measurement of the electrical parameters, and measured values ​​of the electrical parameter related to the concentration of the substance in a composition.

请参阅图13(a),示出一三极式电极的另一实施例,所示的133为一网状窗口,其被置于一网状窗口置放凹处132内,该置放凹处同时具有一工作电极134、 一对电极135、 一参考电极131、 一化学试剂137、 一氯化银层138 等,还有一覆盖层136置于网状窗口上方,以将该置放凹处围成一测量区域。 See FIG. 13 (a), shows a further embodiment of the electrode of three-electrode embodiment, a mesh 133 is shown in a window, which window is placed in a mesh disposed within the recess 132 of the holder recess at the same time having a working electrode 134, a pair of electrodes 135, a reference electrode 131, a chemical agent 137, a silver chloride layer 138, etc., there is a cover layer 136 is placed over the mesh windows, placed in the recess enclose a measurement region. 由图13(b)知该覆盖层136具有一开口以为一流体入口,该流体可由网状窗口直接滴入,该网状窗口133与该置放凹处132是经一亲水性的覆被处理(hydrophilic coating),以利流体容易完全充满整个测量区域。 FIG 13 (b) known in the cover layer 136 having an opening that a fluid inlet, the fluid may be instilled directly window mesh, the mesh 133 with the window recess 132 is disposed through a hydrophilic cover processing (hydrophilic coating), to facilitate easy fluid completely fills the entire measurement region.

图4的绝缘基材40是采用一聚氯乙烯PVC(polyvinyl chloride)、聚丙烯PP (Polypropylene)、聚碳酸酯PC (Polycarbonate)、聚对苯二甲酸亚丁烯基酯PBT (Polybutylene Ter印hthalate)、聚对苯二甲酸乙二醇酯PET (Polyethylene Ter印hthalate)、聚氧化亚苯PP0 (Modified Polyphenlene Oxide)或丙烯腈丁二烯苯乙烯树酯ABS (Acrylonitrile Butadiene Stytene) 等塑胶树脂。 Insulating base 40 of FIG. 4 is the use of a polyvinyl chloride PVC (polyvinyl chloride), polypropylene PP (Polypropylene), polycarbonate PC (Polycarbonate), polyethylene terephthalate butenylene ester PBT (Polybutylene Ter printing hthalate) , polyethylene terephthalate PET (polyethylene Ter printing hthalate), poly phenylene oxide PP0 (Modified Polyphenlene oxide) or acrylonitrile butadiene styrene resin ABS (acrylonitrile butadiene Stytene) and other plastic resins.

第一导电条42是为一先行成型,而于一绝缘基材成型装置中埋入第一导电条42,以形成绝缘基材40。 First conductive strips 42 for forming a first, and the first conductive strips embedded in an insulating base molding device 42 to form an insulating substrate 40. 该第一导电条具有一根导引线来连接该第一输出端和该第二电极端,该电极具有0.3mm-3mm的导电条厚度,该导引线具有0. 2mm-2. 8誦的导电条厚度。 The first conductive strips having a guide wire connected to the first output terminal and the second electrode terminal, the electrode has a conductive strip of a thickness of 0.3mm-3mm, the guide wire having a 0. 2mm-2. 8 recite the thickness of the conductive strip.

本发明的第一导电条42与绝缘基材40是可以一双材料射出方式一次成型。 First conductive strips 42 and the insulating substrate 40 of the present invention is a molding material of one pair emit manner. 第一导电条42是可以改为利用一黏胶以接合至绝缘基材40上。 A first conductive strip 42 can be changed to use a glue bonded to the insulating substrate 40.

又依照另一种可实施的技术方案来看,本发明的一种电化学式传感器的结构,用于检测一流体样本,其包含一绝缘基材40,其具有一第一凹陷空间(如第一凹槽50及一第一贯孔41), 一第一导电装置(如第一导电条42), 容设于第一凹陷空间50,第一导电装置42具有一第一输出端43及相对的一第二电极端44,且以第二电极端44为一第一测试电极44,以及一化学试剂60, 其设置于第一测试电极44上,用以检测该流体样本的一成份,进而产生一测量信号,并经第一测试电极44以输出该测量信号。 In accordance with yet another aspect of an embodiment of view, the structure of an electrochemical sensor according to the present invention for detecting a fluid sample, which comprises an insulating substrate 40 having a first concave space (e.g., a first a first recess 50 and through hole 41), a first electrically conductive means a first conductive means (e.g., first conductive strips 42), accommodated in the space 50 of the first recess 42 has a first output terminal 43 and an opposing a second electrical terminal 44 and a second electrical terminal 44 as a first test electrode 44, and a chemical agent 60, which is disposed on the first test electrode 44 for detecting a component of the fluid sample, thereby generating a measurement signal, via the first test electrode 44 and to output the measurement signal.

如图4所示,该第一导电装置是为一第一导电条42,且该第一凹陷空间包括有一第一凹槽50及一第一贯孔41,并于第一凹槽50内设置第一导电条42,第一测试电极44即设置于第一贯孔41中。 As shown, the first conducting means is a first conductive strip 4 is 42, and the first recess includes a first recess space 50 and a first through hole 41, and disposed within the first recess 50 a first conductive strip 42, i.e., the first test electrode 44 disposed on the first through hole 41. 请参阅图16及图17,该第一导电装置是可以改为一第一导电垫块(Pad)160,且该第一凹陷空间为一第一贯孔161,该第一导电垫块160的第一面为电极工作面积177,该结构还具有以金属膏印刷的一第一印刷导电金属膜162被印刷于一绝缘基材的第二面上, 其与一第一电极输出端164相衔接以接收该测量信号。 See FIG. 16 and FIG. 17, the first conducting means is changed to a first conductive pad (Pad) 160, and the first recess is a first through hole space 161, the first conductive pad 160 the first surface is a work area of ​​the electrode 177, the structure further having a second face with a first metal film 162 printed conductive metal paste is printed on a printed insulating substrate, which is linked with the output terminal of a first electrode 164 to receive the measurement signal. 第一贯孔161还可以具有一扩大凹槽165,以置放第一导电垫块160的一扩大底座176。 First through hole 161 may also have an enlarged recess 165, a first conductive pad disposed to expand a base of 176,160. 本实施例的该印刷导电金属膜162虽为第一测试电极(工作电极)的输出端,但其与工作电极的有效工作面积177无直接相连,故此金属膏印刷导电膜没与待测流体相接触而无化学干扰的顾虑,其印刷尺寸的任何不稳定也不会影响工作电极面积177,故于检测信号测量的稳定性上皆无不良影响,且其可降低该第一导电条42 (请参考图4)的该导引线lead45的电阻值而改善测量稳定。 The printed conductive metal film 162 of the present embodiment, although the first test electrode (working electrode) of the output terminal, but its effective working area of ​​the working electrode 177 of the non-direct, therefore the metal paste film is not printed with conductive fluid to be measured and the contact without concerns of chemical interference, any instability of the print size which will not affect the area of ​​the working electrode 177, so that the stability in the detection signal measurements are no adverse effects, and which can reduce the first conductive strips 42 (see the resistance value of the reference to FIG. 4) of the guide wire lead45 improved stable measurement. 图9(b)的第一测试电极951以塑胶含碳导电复合材料射出成型,其由电极面积951 至电极输出端953的总电阻可降至300Q以下,其比碳膜印刷电极的电阻值已降低数十倍,但其仍比低价金属膏印刷导电膜高数倍(如银胶阻抗可低于10 Q)。 FIG. 9 (b) a first electrode 951 to test the carbonaceous conductive composite plastic injection molding, which is the area of ​​the electrode 951 to the electrode output terminal 953 and the total resistance can be reduced to less 300Q, which has specific resistance value of the carbon printed electrodes reduce the number of times, but still higher than the low conductive film of a metal paste is printed several times (e.g., silver paste may be lower than the impedance of 10 Q). 本发明图16的实施例具有本发明以低价塑胶含碳导电复合材射出成型一无化学干扰、低电极阻抗及面积尺寸稳定的工作电极最佳特性(相对于同样无化学干扰的碳膜印刷电极),但于工作电极以外的其他电极与各电极的导引线Lead均可用低价金属膏印刷导电膜来实现,以使各自优点得以发挥。 FIG embodiment of the present invention to the present invention 16 has low carbon-containing electrically conductive plastic injection molding a composite material without chemical interference, low electrode impedance and the area of ​​the working electrode dimensionally stable optimal characteristic (with respect to the same carbon printing without chemical interference electrode), but other than the working electrode and the other electrode of each electrode guide wire can be realized with Lead Low metal paste printed conductive film, so that the advantages of each to play. 请参照图14(a)为一两电极式的塑胶射出导电电极与金属膏印刷电极的复合实施例,第一导电条(Conductive Strip) 143以塑胶导电材料成型,电极工作面积147于第一贯孔146中成型可获得一低于0.6%误差以内的稳定电极面积, 而对电极(Counter Electrode) 145其无化学干扰与电极面积尺寸稳定的需求故可以金属膏印刷一第一印刷导电金属膜145于绝缘基材141上,以得到更低的电极阻抗而提高电化学传感器的测量准确性。 Please refer to FIG. 14 (a) is a plastic injection-type electrodes composite conductive electrode and a metal electrode paste printing embodiment, first conductive strips (Conductive Strip) 143 of conductive material is molded in plastic, the electrode 147 on the first working surface consistent aperture 146 formed in electrode area can be obtained within a stable below 0.6% error, and the counter electrode (Counter electrode) 145 which is free of chemical interference dimensionally stable electrode area requirement it is possible to print a first metal paste printed conductive metal film 145 on the insulating substrate 141, to obtain a lower electrode impedance measurements to improve the accuracy of electrochemical sensors. 图14(b)为另一个三电极式的复合实施例, 一第二印刷导电金属膜149电极与第一印刷导电金属膜同时被印刷于绝缘基材上以形成一参考电极, 一氯化银修饰层148用以将149 修饰为一氯化银参考电极。 FIG. 14 (b) another embodiment of a composite three-electrode, a second electrode 149 printed conductive metal film is first printed with the conductive metal film is simultaneously printed on the insulating substrate to form a reference electrode, a silver chloride the modified layer 148 to 149 is modified to a silver chloride reference electrode. 本发明实施例图18、图19、图20皆是一塑胶射出导电电极与一印刷导电金属膜电极的复合实施例精神的再延伸。 Example 18 of the present invention, FIG 19, FIG 20 simply shot the lights emitted from the conductive plastic electrode and a printed conductive metal film extending electrode composite further embodiment spirit embodiment.

再按照另一种相当的技术层面来看,请参阅图18,本发明的一种电化学式传感器,用于检测一流体样本,其包含: 一第一导电元件180,其具有一第一测试电极181; —绝缘基材182,具有一第一凹陷空间183,以置放第一导电元件180; —第一印刷导电金属膜184,其与绝缘基材182相接,第一印刷导电金属膜184具一第一输出端185及相对的一第二衔接端186,且以第二衔接端186与第一导电元件180相衔接;以及一化学试剂187,其设置于第一测试电极181上,用以检测该流体样本的一成份,进而产生一测量信号,并经第一测试电极181以输出该测量信号。 According to another further considerable technical perspective, see FIG. 18 A according to the present invention is an electrochemical sensor for detecting a fluid sample, comprising: a first conductive element 180, having a first test electrode 181; - an insulating substrate 182, a space 183 having a first recess to a first electrically conductive member 180 disposed; - printing a first conductive metal film 184, which is in contact with the insulating substrate 182, a first printing conductive metal film 184 having a first output terminal 185 and a second opposite end of adapter 186, and at a second end of the adapter 186 with the first conductive element 180 of convergence; and a chemical agent 187, which is disposed on the first test electrode 181, with to detect a component of the fluid sample, thereby generating a measuring signal, via the first test electrode 181 and to output the measurement signal.

绝缘基材182可以具有一第二凹陷空间188,以置放一第二导电元件189, 且该结构还具有一第二印刷导电金属膜1891,其与第一印刷导电金属膜184 同时被印刷于绝缘基材182上,用以衔接第二导电元件。 The insulating substrate 182 may have a second concave space 188, a second conductive element disposed to 189, and the structure further having a second printing conductive metal film 1891, with the first printing conductive metal film 184 are simultaneously printed on on the insulating substrate 182, for engaging a second conductive element. 又图18的第一凹陷空间183是可以改成如图19(D)所示的型式的第一凹陷空间190,该第一凹陷空间190还与一侧部凹槽191相连, 一第一〃U〃型导电元件1901同时置放于该第一凹陷空间190与该侧部凹槽191中并形成紧配,该第一"IT型导电元件的第一侧1903置于第一凹陷空间190内形成一第一测试电极1903, 一第一印刷导电金属膜193印刷于绝缘基材199上,使第一衔接端193与该第一〃U〃型导电元件1901的第二侧的一导电元件衔接面1904相衔接,以输出该第一测试电极1903的测量信号。且还包括一绝缘层192,用以覆盖第一印刷导电金属膜193与该导电元件衔接面1904,此IT型导电元件的设计以利该第一印刷导电金属膜193能与一第二及一第三印刷导电金属膜19、 198同时印刷于绝缘基材上电极的同一面。绝缘层192并具有一流入凹处194,以供该流体样本 And FIG 18 is a first recess 183 can be changed to space in FIG. 19 (D) represented by a first recess type space 190, the space of the first recess portion 190 is also connected to one side of the recess 191, a first 〃 U〃 shaped conductive elements 1901 disposed in the first recess while the space portion 190 and the side groove 191 and with a tight, the first "first side 1903 IT type conductive element disposed within the space 190 of the first recess a first test electrode 1903 is formed, a first printing conductive metal film 193 on the insulating substrate 199 is printed, the first end of the adapter 193 and the adapter side of a second conductive element of the type of the first conductive member 1901 〃U〃 face 1904 are connected, to output the measurement signal of the first test electrode 1903. 192, and further comprising, a first printed to cover conductive metal film 193 and the conductive member engaging surface 1904, this type of IT to design a conductive element insulating layer to facilitate the first conductive metal film 193 can be printed with 19, while the same 198 printed on the insulating substrate, and a second electrode, a third conductive metal film printed side insulating layer 192 having a recess 194 flows to for the fluid sample

的流入,且绝缘层192具有一呈〃匸〃形幵口195,以形成一测量空间196 (详见图19(a))。 Inflow, and the insulating layer 192 having a shape 〃 〃 Xi Jian-shaped opening 195, to form a measuring space 196 (see FIG. 19 (a)). 该结构还包括有一第二及一第三印刷导电金属膜197、 198, 是与第一印刷导电金属膜193同时被印刷于绝缘基材199上,从图19(b)可以见到一覆盖层1991位于绝缘层192之上,图19(c)显示出一修饰电极1992位于第二印刷导电金属膜197上方。 The structure further comprises a second and a third conductive metal film print 197, 198, with the first printing conductive metal film 193 are simultaneously printed on the insulating substrate 199, a cover layer can be seen from FIG. 19 (b) 1991 over the insulating layer 192, FIG. 19 (c) shows a modified electrode 1992 located above the second printing conductive metal film 197.

如从另一种对等的技术层面来看,请参阅图20(a),本发明的一种电化学式传感器,用于检测一流体样本,其包含: 一塑胶射出导电片200,其具有一第一输出端201及一第二电极端202,且以第二电极端202为一第一测试电极202; —绝缘基材203,是与导电片200相接,且具有一电极贯孔204,以置放第一测试电极202;以及一化学试剂205,其设置于第一测试电极202上, 用以检测该流体样本的一成份,进而产生一测量信号,并经第一测试电极202 以输出该测量信号。 As another peer from the technical point of view, see FIG. 20 (a), the present invention is an electrochemical sensor for detecting a fluid sample, comprising: a plastic injection conductive sheet 200 having a a first output terminal 201 and a second electrical terminal 202, and a second electrical terminal 202 of a first test electrode 202; - an insulating substrate 203, is in contact with the conductive sheet 200, and an electrode having a through hole 204, the first electrode 202 disposed in the test; and a chemical agent 205, which is disposed on the first test electrode 202 for detecting a component of the fluid sample, thereby generating a measuring signal, and via the first electrode 202 to output the test the measurement signal.

该结构的第一测试电极202可以位于导电片200的一凸出部位202上, 以置入绝缘基材203的电极贯孔204。 The first test electrode structure 202 may be located in a projecting portion 200 of the conductive sheet 202, the insulating base inserted to the through hole 203 of the electrode 204. 该结构还可以包括一修饰电极206,设置于一第二印刷导电金属膜207上,而成为一参考电极206,由图20(b)即可以看出一覆盖层209位于二绝缘层208的上方。 The structure may further comprise a modified electrode 206 disposed on a second printed conductive metal film 207, becomes a reference electrode 206, FIG. 20 (b) that is a cover layer 209 can be seen positioned over the second insulating layer 208 .

如从另一种对等的技术层面来看,请参阅图20 (C),本发明的一种电化学式传感器,用于检测一流体样本,其包含:以一塑胶导电复合材料成型的一平坦导电基材2001,其具有一第一输出端2011及一第二电极端2021,且以第二电极端2021为一第一测试电极2021; —第一绝缘层2031,是与该平坦导电基材2001相接,且该第一绝缘层2031具有一电极贯孔2041以形成第一测试电极2021的一凹陷电极面积2041,并以该电极贯孔形成一置放凹处2041以为一化学试剂2051的置放处,该化学试剂用以检测该流体样本的一成份,进而产生一测量信号,并经第一测试电极2021传至该第一输出端2011 以输出该测量信号。 As another peer from the technical point of view, see FIG. 20 (C), the present invention is one kind of electrochemical sensor for detecting a fluid sample, comprising: a planar conductive composite to a molded plastic the conductive substrate 2001 having a first output terminal 2011 and a second electric terminal 2021 and second terminal 2021 is electrically testing a first electrode 2021; - a first insulating layer 2031, a conductive substrate with the flat phase 2001, insulating layer 2031 and the first electrode having a first through hole 2041 to form a test electrode recessed area 2021 of the electrode 2041, and to the through hole electrode is formed a recess 2041 that placed a chemical agent 2051 disposed at a component of the chemical agent used for the detection of the fluid sample, thereby generating a measurement signal, the first test electrode 2021 and transmitted via the first output terminal 2011 to output the measurement signal. 当然,此时的结构还可以包括一氯化银层2061,设置于一第二印刷导电金属膜2071上,而成为一氯化银参考电极。 Of course, in this case the structure may further comprise a silver chloride layer 2061 disposed on a second printed conductive metal film 2071, becomes a silver chloride reference electrode.

本图20的发明实施例以导电片(Conductive Sheet)代替前实施例导电条(Conductive Strip)的主要目的为获得有利于双料塑胶射出时的制造方法,于塑胶射出过程中需将塑胶料注入塑胶模具的膜穴中并以保压以为塑胶 Embodiment of the invention the present FIG. 20 according to the conductive sheet (Conductive Sheet) The main objectives of the conductive strip embodiment (Conductive Strip) for the former instead of obtaining facilitate manufacturing method when the double plastic injection, in the plastic injection molding process required to the plastic material injected into the plastic caveolae mold and pressure to ensure that the plastic

料完全饱满于整个成品,其需将原模穴中原有的空气挤出以为塑胶料容易注入模穴中,前实施例的导电条被绝缘基材三面包住,只剩一面又需留为塑胶射出的入料口,这样于射出成形中空气被包住不易挤出而造成射出成品的塑胶料不饱满而造成成品缺料而不良,本实施例的导电片与绝缘基材皆可四面与模具钢材接触,钢膜侧壁可易于制造一钢膜出气孔以将空气引出模穴。 Completely full throughout the finished material, which required the original air out of the mold cavity that any original easily injected plastic material in the mold cavity, the conductive Top embodiment is an insulating substrate live three sides, leaving only the left side and the need of plastic emitted into the discharge port, so that the air in the injection molding is difficult to wrap the extrudate caused by poor emitted finished plastic material is not full finished material shortage caused by the conductive sheet and the insulating substrate of the present embodiment is surrounded by the mold Jieke contacting the steel, the steel can be easily manufactured film sidewall a steel membrane to the air extraction vent mold cavity.

请参照图15,其为一习知手提式电化学计量器150,用于插入本发明的一的电化学传感器试片841 (如本发明实施例的图8、图10、图11、图13及图18至图20、)至该剂量器的一卡槽153内,待测流体由流体入口89引入至传感器内后经一测量时间,该计量器会显示待测流体分析物的生化成分数值。 Referring to FIG. 15, which is a conventional portable electrochemical meter 150, for insertion into the electrochemical sensor of the present invention, a test strip 841 (as FIG. 8 embodiment of the present invention, FIG 10, FIG 11, FIG 13 and FIG. 18 to FIG. 20,) to the inside of a unit dose of slot 153, fluid from the fluid to be measured over a measuring time is introduced to the fluid to be measured within the sensor component biochemical analyte values ​​inlet 89, the meter will display .

综合上述结构说明后,特再补充如下实务数据说明,可得更明了本发明的优点。 After the integrated structure described above, and then add the following substantive data Laid described, it may be more apparent advantages of the present invention. 如图9 (b)所示为本发明塑胶射出成型电极的传导阻抗的影响因素即导体宽度(W)及导体厚度(t)的示意图,本发明所完成的导电条(Conductive Strip)的传导截面积A的导体厚度tl可由0.2-3mm依需要任意设计,其与传统印刷电极的导体膜(Conductive Film)厚度仅为数um至数十um (如图2 的拋弃式印刷电极20的印刷厚度),两者厚度厚度相差数百倍,若以相同导体长度与宽度及同样使用含碳材低价材质(无化学干扰)相较,本发明可使导体阻抗大大的降低。 FIG. 9 (b) that is oriented as shown in the conductor width (W) factors plastic injection molding schematic conductive electrode impedance and conductor thickness (t) of the present invention, the present invention is accomplished by a conductive strip (Conductive Strip) conduction cut a conductor thickness tl area may be arbitrarily designed as needed 0.2-3mm, with the conventional printed electrode conductor film (conductive film) to a thickness of only several tens of um um (the thickness of the disposable print printed electrode 20 of FIG. 2) , both the thickness difference between the number of times, if the same width and the same length of the conductor material using the carbonaceous material of low (no chemical interference) in contrast, the present invention can greatly reduce the impedance of the conductor. 由阻抗公式: Impedance by the formula:

导电阻抗R= P丄/A: P丄/ (Wt) Shang conductive impedance R = P / A: P Shang / (Wt)

其中P:电阻是数L:导体长度A:导体截面积 Wherein P: is the number of resistance L: length of conductor A: cross sectional area of ​​the conductor

A=导体宽度(W).导体厚度(t) A = conductor width (W). Conductor thickness (t)

当然网版印刷方式可选择网版的厚度或网目数来增加导电膜的厚度以降低阻抗,但是当印刷膜厚度增高至一定限度以上,导电膏于印刷时因太厚而容易向外扩散造成电极工作面积的不稳定,工作面积不稳定而影响测量不稳定比传导阻抗高的影响更为严重,故网版印刷式电极的导电膜无法任意增厚。 Of course, a thickness by screen printing or screen selectable mesh screen to increase the thickness of the conductive film to reduce the resistance, but when the printed film thickness increased to more than a certain limit, because of a conductive paste to a printed thick easily cause outdiffusion unstable working electrode area, the work area is unstable unstable measurement impact is more serious than the influence of high-impedance conductivity, the conductive film so that screen printing can not be any thicker electrode.

本发明实施例如图9 (b)所示,以Carbon占30%比率的塑胶导电复合材料成形的一厚度tl为0.6mm及宽度W为1.5mm的导电条,其电极阻抗值若以分段测量,其一由电极表面积951至其电极背面952的电阻实际测试值为20-50Q (以三用表Multimeter测量,因三用表的测试棒与电极的接触面积大 Embodiment of the present invention, for example, FIG. 9 (b), the percentage of a thickness tl to Carbon conductive plastic composites formed of a 30% ratio of 0.6mm and 1.5mm width W of the conductive strip, which segment In terms of measuring electrode impedance value , one actual test by the resistance of the electrode surface area of ​​951 to the back surface of the electrode 952 is 20-50Q (Multimeter measurement in three tables, three tables because test bars in contact with the electrode area is large

小不同而影响测量电阻值由20-50Q变化),由电极背面952至输出端953的30mm长度的Lead段的电阻实际测试值为100-220Q,其总合电组低于300Q。 Varies little impact value measured by the resistance change 20-50Q), Lead resistance actual test section by 30mm length back electrode 952 to the output terminal 953 is 100-220Q, electrically group with a total of less than 300Q. 相对于传统碳基(Carbon Base)的导电碳膜印刷电极的阻抗值由数KQ至十KQ是一大改善,此可由参考美国专利第5,985,116号专利的权利要求2的技术内容作相对比较,其以印刷导电碳膜的电极阻抗值不高于IOKQ为其专利要求值,与本发明实施例有数十倍的差异,足见本发明能以低价碳基(Carbon Base)材质获得相当成效的电极的阻抗改善。 With respect to the resistance value of the conductive printed carbon-based electrodes of the conventional carbon (Carbon Base) by the number of ten KQ KQ is a big improvement, this technical content 2 may be claimed by reference U.S. Pat No. 5,985,116 patent requires relatively, electrode impedance value which is a printed conductive carbon film is not higher than its patent claims IOKQ value, several times the difference between the embodiment of the present invention embodiment, the present invention which shows a carbon group can be obtained at low (carbon base) materials quite effective electrode impedance improvement.

如前电化学测量电流公式所述工作电极表面积的尺寸大小与测量输出信号成线性比例关系,故工作电极表面积的尺寸的影响电化学测量的稳定。 As electrochemical measurement current formula and the measured size of the work output signal linearly proportional to the surface area of ​​the electrode, so that stable on the electrochemical measurement of the size of the electrode surface area. 本发明实施例其电极表面积由贯孔面积决定,塑胶钢模开好后其模具上电极的钢模尺寸是固定的不会变化,为增加生产效益釆用多模穴塑胶模具可一次射出多片电极,要将多模穴塑胶模具的尺寸误差控制在0.3%误差以下是塑胶钢模的一般技术,另外塑胶射出有縮水率的变异性,其縮水率的变异性存在于不同射出批次,塑胶縮水率因不同材质而不同,例如PC、 ABS、 PBT等高尺寸稳定性的材质的塑胶总縮水率小于0.5%,而其变异性只是总縮水率于不同生产批间的差异,如塑胶射出成型后的某一批缩水例为0.45%而另一批为0.38% 则其间的差异为0.07%, 一般縮水率间的差异不会高于0.3% (固定选用同一塑胶材质),则以塑胶射出电极的尺寸总误差为塑胶钢模尺寸误差加上塑胶縮水率的差异,则本发明可达到电极工作表面积尺寸误差在0.6%以内。 Embodiment of the invention which is determined by the through hole electrode surface area, the size of the steel good plastic-steel upper mold opening which is a fixed electrode does not change, to increase production efficiency may preclude a multi emitting multi-piece plastic mold cavity electrode, to a multi-cavity plastic mold size error control is less than 0.3% error in the general art plastic-steel, with a further plastic injection shrinkage variability, and variability in shrinkage which is present in different batches emitted, plastic due to different shrinkage of different materials, for example, the total shrinkage plastic material PC, ABS, PBT high dimensional stability less than 0.5%, while the total shrinkage variability in only differences between different production batch, such as plastic injection molding Example shrink after a batch of 0.45% to 0.38% and the other batch of the difference therebetween was 0.07%, the difference between the shrinkage is generally not higher than 0.3% (the same plastic material fixed selection), places the plastic injection electrode the total size of the error plus the difference in the dimensional error plastic-steel plastic shrinkage, the present invention can achieve working electrode surface area is less than 0.6% dimensional error.

而传统网版印刷电极方式网版的选用, 一般有两种方式, 一为软性的丝版,另一为硬的钢版。 The traditional way of screen printing electrode selection screen, there are two ways, one is a flexible wire version, another version of a hard steel. 控制丝版的目数与厚度,或控制钢版的厚度皆可改变导电膜的印刷膜厚度,更而改变印刷时施加于网版的力量也可改变印刷膜厚度。 Version control wire mesh and a thickness, the thickness of the stencil or control changes Jieke printed film thickness of the conductive film, the power applied to the screen printing may also change when the film thickness is more varied printing. 一般多模穴的丝质网版,其网版薄膜的成型尺寸与误差较钢版大,且于印刷中软性丝版的薄胶膜会渐渐剥落而扩孔,导电膜于印刷后干固前会向外扩散,导电膜愈厚扩散愈大,故于印刷小尺寸的电极面积(如血糖检测试片的每一电极面积小于2mm x 2mm)时,其印刷误差界于5-10%是正常规格, 要控制在5%以下是可能的,但其管控需严格,而检验频繁会造成生产效率的降低而成本增加。 Usually multi-cavity silk screen, its screen size and the film forming large error compared with steel plate, and the flexible wire in the printing plate and the thin film will gradually peeled reamer, after printing a conductive film on the front dryness when diffuses outwardly greater diffusion conductive film thicker, so in a small-sized area of ​​the printed electrode (e.g., electrode area of ​​each blood glucose test strip is less than 2mm x 2mm), which is a normal printing error bounds 5-10% specifications, should be controlled at below 5% is possible, but it needs strict control, and test frequently result in reduced productivity and increased costs. 虽钢版式的网版尺寸稳定,但因钢版印刷位置为全露空, 其印刷导电膏厚度很厚虽能降低电阻,但太厚使导电膏会向外扩散大,电极 Although steel screen layout dimensionally stable, but the printing position for the whole steel free air, which conductive paste is printed thick, although the thickness can be reduced resistance, but too thick so that the conductive paste may spread outwardly large, electrode

面积因扩散造成的尺寸误差比丝版更大。 Area due to diffusion caused by errors greater than the size silk version. 除传统网版印刷方式外,也可以厚膜技术(Thick Film)来成型电极面积,其电极尺寸稳定,但厚膜生产的导电膜的厚度很薄使碳膜电极导电电阻很高至十数KQ 。 In addition to conventional screen printing, thick film technology may be (Thick Film) molded to the electrode area, the electrode dimensionally stable, but the thickness of the conductive thin film produced by a thick film electrode conductive carbon film resistors ten high number KQ .

另外,不论是网版印刷或厚膜技术成型的导电膜电极,其真正的电极有效工作面积不是只由印刷的电极面积的尺寸单独决定,因电极面积与引出电极至输出端的引导线(Lead)需相连,需另印上一层绝缘层以覆盖引导线, 引导线未全覆盖而残留下的引导线面积亦全为电极的有效工作面积,如图2 与图3为美国专利第5,985,U6号的情形,印刷的工作电极面积221需由引导线20引接至输出接点222,真正的工作电极面积无法单独由221面积决定, 需再由绝缘层223印刷位置共同决定,因绝缘层的网版尺寸误差、绝缘层与导电层间的印刷错位或绝缘层印刷后胶的扩散等皆会改变电极的真正有效工作电极面积22。 Further, both the conductive film electrode screen printing or thick-film technology molding, the real electrode active work area is not determined only by the size of the electrode area is printed individually by the electrode area of ​​the lead guide wire electrode to the output terminal (Lead) connected required, subject to the printed insulating layer to cover the guide wire, the guide wire does not cover all the area under the guidewire remains valid also for the full working area of ​​the electrodes 2 and 3 of U.S. Patent No. 5,985, U6 case number, the working electrode area printed 221 needs connection of the guide wire 20 to output contact 222, the real working electrode area can not be determined solely by the 221 area, the need then determined by 223 printing positions insulating layer, because the halftone insulating layer truly effective working electrode area offset printing or after printing diffusion insulating layer of plastic between the dimensional error, the insulating layer and the conductive layer of the electrode 22 will change mused.

由上叙述得知网版印刷或厚膜技术成型的导电膜电极,决定工作电极真正有效工作面积的因素太多,且各项因素基本误差又大,相对于本发明于工作电极面积的稳定因素既单纯又变异小,两者有很大的差异。 Obtained by the above described conductive film or a thick film electrode printing technology HowNet molding, much of the working electrode determines the effective working area of ​​the real factors, and the basic error factor and large, the present invention is a stabilizing factor with respect to the working electrode area both simple and small variation, the two are very different. 因电化学传感器的测量信号大小与电极真正有效工作面积成线性正比关系,足见本发明于工作电极面积的稳定上对电化学传感器的测量再现性的准确度会有很大的贡献。 Due to the magnitude of the signal measured with an electrochemical sensor electrode truly effective working area of ​​the linearly proportional relationship, which shows the present invention on a stable working electrode area required accuracy of reproducibility of the electrochemical sensor will be a great contribution.

本发明实施例以低价含碳塑胶导电复合材料射出成形的导电条电极总电阻值小于300Q,其比同为无化学干扰的碳膜印刷电极的阻抗(数KQ至十数KQ)降低了数十倍,但其仍比用低价金属膏(如银胶)的印刷导电金属膜电极(可小于10Q)的电极阻抗仍大很多。 Embodiment of the present invention, the total resistance of the electrode busbars embodiment at low carbonaceous conductive composite plastic injection molding is less than 300Q, which is the impedance (several ten number KQ KQ) printed carbon electrode without chemical interference is reduced compared with the same number electrode impedance times, but still higher than the printed conductive metal film electrodes with low valent metal paste (e.g., silver paste) (which may be less than 10Q) is still much greater. 本发明实施例的总电极电阻小于300 Q,其几乎全降在塑胶导电条的引导线段,此可再加一道程序以传统电极印刷方式,在工作电极的背面(如图16的电极工作表面积的背面164)至输出端间印刷一低价金属膏(如银胶)的导电膜,则总阻抗值可降至50Q以下, 且金属膏导电膜在电极工作面积的背面,不会与待测流体接触故无化学干扰。 The total electrode resistance embodiment of the present invention is less than 300 Q, which is almost full drop of the guide segment of the conductive strip of plastic, this can be combined with an electrode in a conventional printing procedure, the back surface of the working electrode (electrode 16 of FIG working surface area of ​​the back surface 164) to the end of the printed output between a low valent metal paste (e.g., silver paste) conductive film, the total impedance value can be reduced to less 50Q, the conductive paste and the metal electrode film on the back surface of the work area, with the fluid to be measured does not therefore, the contact-free chemical interference.

另工作电极以外的测试电极如参考电极(Reference Electrode)与对电极(Counter Electrode),其无化学干扰问题(只工作电极才有化学干扰问题), 且其电极面积大小并不会影响传感器的测量电流故不需顾虑印刷电极尺寸的 Another measurement test electrode than the working electrode as the reference electrode (Reference Electrode) and the counter electrode (Counter Electrode), which without chemical interference (only the working electrode only chemical interference problems), and which does not affect the size of the electrode area of ​​the sensor therefore, the current does not need to worry about the size of the printed electrode

不稳定,所以参考电极、对电极及各电极的导引线可用低价金属膏印刷导电膜来成形以获得比塑胶射出电极更低的阻抗,而只将工作电极以塑胶含碳导电复合材料成形一导电条以求取工作电极的工作面积稳定、无化学干扰与电极电阻值(比传统印刷碳膜电极小数十倍)等优点。 Unstable, the reference electrode, a low valent metal paste printed conductive film can be used to guide the wire electrode and each electrode is formed by plastic injection ratio to obtain a lower electrode impedance, but only the working electrode carbonaceous conductive plastic composite material shaped a conductive strip in order to obtain a stable working surface of the working electrode, no chemical interference with inter electrode resistance value (than conventional printed carbon electrode fractional times) and so on. 此整合以含碳导电复合材料塑胶射出成型一工作电极,而以印刷低价导电金属膜成形其他电极与各电极的导引线,以使各自优点得以发挥亦为本发明实施例的特点。 This integrated to the carbonaceous conductive composite plastic injection molding a working electrode, and the conductive metal film to the printing Low guide wire forming each electrode and the other electrode, so that the characteristics of the respective advantages are also play an embodiment of the present invention.

本发明的制造方法包含提供一射出成型装置(模具),提供一绝缘基材,注入一塑胶导电的成型材料,以及提供一化学试剂等。 Manufacturing method of the present invention to provide a molding material comprising an injection molding apparatus (mold), providing an insulating substrate, a plastic injection electrically conductive, and to provide a chemical reagent. 藉由本发明所提供的电化学式传感器的制造方法及其结构,即能获致该电化学传感器的一尺寸误差极低的工作电极的表面积,由于工作电极表面积的尺寸与传感器的测量信号值成线性正比关系,故而本发明能大大提高电化学式传感器测量信号的再现性稳定度(Reproducibility)。 By manufacturing method and structure of an electrochemical sensor according to the present invention is provided, i.e., the surface area can be obtained a low error working electrode of the electrochemical sensor actuator size, since the measurement signal value to a linear dimension of the sensor is proportional to the surface area of ​​the working electrode relationship, and therefore the present invention can greatly improve the reproducibility of stability (reproducibility) electrochemical sensor measurement signal.

又因本发明由塑胶射出的方式成形电极的导电条(Conductive Strip),其导电条截面积的厚度由0.2-3mra尺寸可依需要而设计,此厚度是传统印刷导电膜(Conductive Film)式电极以印刷技术所不可能达到的,两者厚度相差数十至百倍,因而本发明的电极电阻比印刷式导电碳膜(Carbon Conductive Film)的电极电阻小了数十倍(若因为避免工作电极的化学干扰而同样用低成本的碳材为工作电极的导电材料),此低电极电阻降低了传感器因测量电流所产生的传递电压降,而改善了测量准确度。 Because of the conductive bar (Conductive Strip) electrode of the invention by a plastic injection molding manner, the thickness of the conductive strip cross-sectional area may vary depending on the needs and design 0.2-3mra size, the thickness of this film is the traditional printed conductive (Conductive Film) electrode in printing technology not attainable, a difference of a thickness of several tens to two hundred times, so the electrode resistance of the present invention is smaller than the resistance of the electrode type conductive printed carbon (Carbon conductive Film) several times (as if to avoid working electrode chemical interference cost the same carbon material as the working electrode of electrically conductive material), this reduces the low-resistance transfer electrode sensor due to a voltage drop generated by a current measurement, measurement accuracy is improved.

本发明是用塑胶射出方式成形传感器的电极与绝缘基材,于相同的塑胶射出程序可将传感器所需要的其他机构一次成形,如将待测流体入口、流体毛细流动通道凹槽、毛细对流气孔、化学试剂置放凹处等多种结构一次成形,不但可减少元件组装数目以降低成本,更可降低多元件的组装误差。 The present invention is a plastic injection molding embodiment of the sensor electrode and the insulating substrate, is emitted to the same procedure may be primary molded plastic bodies of other desired sensor, such as the inlet fluid to be measured, the flow passage of capillary grooves, the capillary pores convective , chemical reagents and other structures disposed recesses primary molding, not only the number of assembly components can be reduced to reduce costs, but also reduce assembly error of a multi-element.

本发明还结合了塑胶导电射出电极与传统印刷导电金属膜(Metal Conductive Film)电极的各自优点,将塑胶导电射出电极的方法用于成形工作电极(Working Electrode)以得到稳定的电极面积与无化学干扰,而以传统低价印刷导电金属膜(如银胶)用于成形参考电极(Reference Electrode)、对电极(Counter Electrode)与各电极的导引线等以得到比塑胶射出导电条更低的电阻值,以发挥两者的各自优点。 The present invention also incorporates a conducting plastic electrode and emitted conventional printing conductive metal film (Metal Conductive Film) the respective advantages of the electrode, a conductive plastic emitted method for forming an electrode the working electrode (Working Electrode) to give a stable electrode area and no chemical interference, while conventional low printing conductive metal film (e.g., silver paste) for forming a reference electrode (reference electrode), counter electrode (Counter electrode) each electrode and the guide wire and the like to give the plastic injection ratio lower conductive strip resistance values ​​to play their respective advantages of both. 依本发明制成的电化学式的电极及传感器,可适用于各式的电化学式检测电极、流体生化成分传感器(如污水、农药含量成分检测试片)、生物传感器、各式拋弃式检测试片(如血糖、尿酸检测试片)等方面的应用。 Under this electrode and the electrochemical sensor of the invention is made applicable to a variety of electrochemical detection electrode, biochemical fluid composition sensor (e.g., water, pesticide content of the component test strip), biosensor, various types of disposable detection test application sheet (e.g., glucose, uric acid test strip) and other aspects.

综上所述,本发明确实能以一新颖的方式,藉由置放一绝缘基材到射出成型装置内,以形成第一导电条于该绝缘基材的一第一凹槽内,而能利用该绝缘基材的第一贯孔,以形成该第一导电条的一工作电极。 In summary, the present invention is actually capable of a novel manner, by placing an insulating base material into the injection molding apparatus to form the first conductive strips in a first recess of the insulating substrate, but can using the first through hole of the insulating substrate to form a working electrode of the first conductive strip. 并且所使用的塑胶导电的成型材料的注入该射出成型装置内,以获得该第一导电条与该塑胶基材彼此间的一紧配效果,并极适合工业上的生产。 And conductive plastic injection molding material used in the molding apparatus which is emitted, the first conductive strips to obtain a tight fit results between the plastic substrate and from each other, and very suitable for the production on an industrial.

Claims (17)

  1. 1. 一种电化学式传感器的制造方法,其特征在于,包含:提供一个射出成型装置;提供一种绝缘基材,其具有一个沿绝缘基材的长度方向延伸的第一凹槽及一个位于绝缘基材一端上的第一贯孔,且该第一凹槽和该第一贯孔项连通; 将该绝缘基材送入该射出成型装置内;注入一种塑胶导电的成型材料至该射出成型装置内,以于该第一凹槽内形成一个第一导电条,而该第一导电条具有一个第一输出端及相对的一个第二电极端,且以该第二电极端为一个第一测试电极,该第一测试电极即于该第一贯孔中形成;以及提供一种化学试剂,其设置于该第一测试电极上,用以检测一种流体样本, 该化学试剂即与该流体样本的成分反应,进而产生一测量信号,并经该第一测试电极以输出该测量信号。 1. A method of manufacturing an electrochemical sensor, characterized in that, comprising: providing an injection molding apparatus; provide an insulating substrate having a first groove along a longitudinal direction of the insulating base and extending in the insulating a first through hole on one end of the substrate and the first recess and the first through hole communicating item; the insulating base material fed into the injection molding apparatus; injecting a plastic molding material to the electrically conductive injection molding the means within the first recess to form a first conductive strip, and the first conductive strip having a first output terminal and a second electrical terminal opposite to, and at the second electrical terminal of a first test electrode, i.e., the first test electrode formed on the first through hole; and to provide a chemical agent, which is disposed on the first test electrode, for detecting a sample of a fluid, i.e. the fluid with the chemical agent sample reaction component, thereby generating a measuring signal, via the first test electrode and to output the measurement signal.
  2. 2. 如权利要求1所述的方法,其特征在于,该第一测试电极输出该测量信号至一个计量器,且该塑胶导电材料是由一种树酯基材分别与一种导电碳材或一种金属粉所合成,该树脂基材为一种热成形的塑胶树酯或一种环氧树脂,而该塑胶导电材料的该导电碳材为碳黑、石墨、碳纤维或纳米碳管,该金属粉为金、白金、钯或铑,且该导电碳材具有该树脂基材的3〜60%的重量比,而该金属粉具有该树脂基材的0.1〜5%的重量比,其中该环氧树酯与该导电碳材或该金属粉搅拌呈一液态而注入该绝缘基材的该第一凹槽及该第一贯孔,该第一导电条即与该绝缘基材形成一紧密配接。 2. The method according to claim 1, wherein the first test electrode outputs the measurement signal to a meter, and the conductive material is made of a plastic resin substrate, respectively, and an electrically conductive carbon material or synthesized powder metal, plastic resin or an epoxy resin substrate is formed of a thermally, electrically conductive carbon material and that the plastic material is conductive carbon black, graphite, carbon fibers or carbon nanotubes, the metal powder such as gold, platinum, palladium or rhodium, and the conductive carbon material having 3~60% by weight ratio of the resin substrate, and the metal powder having 0.1~5% by weight ratio of the resin substrate, wherein the epoxy resin and the conductive carbon material or the metal powder was stirred for a liquid injected into the insulating substrate and the first recess and the first through hole, i.e. the formation of a first conductive strips and the insulating substrate in close mating.
  3. 3. —种电化学式传感器,用于检测一流体样本,其特征在于,包含: 一种绝缘基材,其具有一个沿绝缘基材的长度方向延伸的第一凹槽及一个位于绝缘基材一端上的第一贯孔,且该第一凹槽和该第一贯孔相连通;一个第一导电条,是与该绝缘基材相连接,以将该第一导电条设置于该第一凹槽内,而该第一导电条具有一个第一输出端及相对的一个第二电极端,且以该第二电极端为一个第一测试电极,该第一测试电极即设置于该第一贯孔中;以及一种化学试剂,其设置于该第一测试电极上,用以检测该流体样本成份, 进而产生一个测量信号,并经该第一测试电极以输出该测量信号。 3 - Species electrochemical sensor for detecting a fluid sample, wherein, comprising: an insulated substrate, a first end having a groove along a longitudinal direction of the insulating base and extending in the insulating base material a first through hole, and the first recess and the first through hole communicating; a first conductive strips, is connected to the insulating substrate to the first conductive strips disposed on the first recess slot, and the first conductive strip having a first output terminal and a second electrical terminal opposite to, and at the second electrical terminal of a first test electrode, i.e., the first test electrode is disposed on the first through hole; and a chemical agent, which is disposed on the first test electrode, for detecting the fluid sample components, thereby generating a measuring signal, via the first test electrode and to output the measurement signal.
  4. 4. 如权利要求3所述的电化学式传感器,其特征在于,还包含一个绝缘层, 用以使该第一导电条获得绝缘效果,且该第一测试电极是为该第一导电条的一个引出点,而该第一测试电极是为一工作电极;且该电化学式传感器还具有一个第二导电条,而该绝缘基材设有一个第二凹槽及一个第二贯孔,以分别设置该第二导电条及该第二导电条的一个第二测试电极,其中,第二测试电极为一对电极。 4. The electrochemical sensor according to claim 3, characterized by further comprising an insulating layer, the first conductive strips for causing an insulating effect is obtained, and that the first test electrode is a first conductive strip exit point, and the first test electrode is a working electrode; and the electrochemical sensor further having a second conductive strip, and the second insulating substrate is provided with a recess and a second through hole, are provided to a second conductive strip and said second conductive strips of the second test electrode, wherein the second pair of electrodes is a test electrode.
  5. 5. 如权利要求4所述的电化学式传感器,其特征在于,还具有一个第三导电条,而该绝缘基材设有一个第三凹槽及一个第三贯孔,以分别设置该第三导电条及该第三导电条的一个第三测试电极,该第三测试电极即为一个参考极。 5. The electrochemical sensor as claimed in claim 4, wherein, further comprising a third conductive strip, and the insulating substrate is provided with a third recess and a third through hole, are provided to the third a third conductive strip and the conductive strips of the third test electrode, the third electrode is the reference electrode test.
  6. 6. 如权利要求4所述的电化学式传感器,其特征在于:绝缘基材上具有一个网状窗口置放凹处,以置放一网状窗口,该置放凹处的底部或侧部置有该测试电极与该化学试剂,以形成流体的一测量区域;该电化学式传感器还有一个覆盖层以置于该网状窗口上方并与该绝缘基材相接,该覆盖层具有一个开口以露出一部份网状窗口以为待测流体的滴入处;该网状窗口为一种织布或塑胶网片材质,以使该窗口呈现网孔状,且具有60〜300网目数;及/或该网状窗口与该置放凹处是经过亲水性的覆被处理,以利流体容易完全充满整个测量区域。 6. The electrochemical sensor as claimed in claim 4, wherein: the recess has a mesh window disposed on the insulating substrate, a mesh placed in the window, the recess disposed opposite the bottom or side the test electrode has the chemical agent, to form a measurement region fluid; the electrochemical sensor and a cover layer is placed over the mesh in contact with the insulating window and the substrate, the cover layer having an opening to exposing a portion of the window that was dropped at mesh fluid to be measured; the window as a woven fabric or mesh of plastic mesh material, so that the mesh-like presentation window, and having 60~300 mesh network; and / or the mesh window recess is disposed through the hydrophilic coating is processed to facilitate easy fluid completely fills the entire measurement region.
  7. 7. 如权利要求4所述的电化学式传感器,其特征在于,该绝缘基材具有一个流入凹处,以供该流体样本的流入,而该绝缘基材具有一个流体入口,该流体入口、该流入凹处及该第一贯孔为一体成型。 7. The electrochemical sensor of claim 4, wherein the fluid inlet, characterized in that the insulating substrate has a recess flows, for the inflow of the fluid sample, and the insulating substrate having a fluid inlet, the flows first through hole and said recess integrally molded.
  8. 8. 如权利要求7所述的电化学式传感器,其特征在于,由置放在绝缘基材上覆盖层和流体入口及该流入凹处形成毛细流动通道与测量区域。 8. The electrochemical sensor according to claim 7, wherein the fluid inlet, and a layer disposed on the insulating substrate and covering the recess forming capillary flow passage flows into the measurement area.
  9. 9. 如权利要求8所述的电化学式传感器,其特征在于,该绝缘基材的该流入凹处具有一个置放凹处,以供该化学试剂的完整置放,而该测量区域的一个测量底面是与该第一测试电极的一个电极顶面形成一个高度差,且电极顶面与该置放凹处形成一个合成底面,以供该化学试剂的涂布,且该化学试剂是以一种定量型式注入该置放凹处,而于该合成底面上形成该化学试剂的均匀厚度。 9. The electrochemical sensor as claimed in claim 8 and a measurement of the measurement region, characterized in that the recess flows into the insulating substrate having a recess disposed, for complete placement of the chemical agent, the bottom surface is formed with a top surface of the first electrode of a test electrode height difference, and the top surface of the electrode forming a composite recess bottom surface of the holder, for applying the chemical agent, and which is one kind of chemical agent quantitative injection type recess of the holder, and in the synthesis of a uniform thickness of the bottom surface of the chemical reagent.
  10. 10. 如权利要求8所述的电化学式传感器,其特征在于:该绝缘基材具有一个抵顶凸块,以抵顶该覆盖层,并利用该抵顶凸块来隔离,使该流体样本得以避免接触该覆盖层的黏胶;该覆盖层是为一种塑胶片或一种透明或半透明材质或一种织布,其中该塑胶片的该覆盖层并具有一层印刷导电金属膜,并以该导电金属膜作为一个第二或一个第三测试电极;该电化学式传感器还具有一个第四测试电极,用以检测待测流体的流量, 而使该第四测试电极成为一个流量检测电极;该透明或半透明材质的该覆盖层具有一个不透明漆部分,以利使用者观察该流体样本的流动情形,而该覆盖层印有一个方向记号,以提醒该使用者其为一个流体入口处;该织布或塑胶网片材质的该覆盖层呈现网孔状,且具有60~300网目数;该测量区域与覆盖层内侧面是经过亲水性的覆被处理 10. The electrochemical sensor according to claim 8, wherein: the insulating substrate has a protrusion abutting block, so as to abut against the covering layer, and by using the abutting spacer bumps, so that the fluid sample is viscose avoid contact of the cover layer; the cover layer as a plastic film or of a transparent or translucent material or one woven fabric, wherein the covering layer and the plastic film having a layer of printed conductive metal film, and in the conductive metal film as a second electrode or a third test; the electrochemical test sensor further includes a fourth electrode for detecting the flow of fluid to be measured, the test electrode and the fourth electrode into a flow rate detection; the transparent or translucent material of the cover layer has a portion opaque paint, in order to facilitate the user to observe the flow condition of the fluid sample, and the cover layer printed with a direction mark, to remind the user which is a fluid inlet; the woven fabric or plastic mesh material of the cover layer exhibits a mesh shape, and has a mesh number of 60 to 300; within the measurement zone and through the cover layer side surface is processed to be hydrophilic coating 以利流体容易完全充满整个测量区域;及/或该绝缘基材具有一个第一入口侧及相对的一个第二气孔侧,是分别作为该流体入口及形成一个气孔开口,且该气孔开口以利于该流体样本作毛细流动。 To facilitate easy fluid completely fills the entire measurement region; and / or the insulating substrate having a first side and an opposite inlet hole and a second side, respectively, as the fluid inlet opening and an air hole is formed, and the hole to facilitate opening for capillary flow of the fluid sample.
  11. 11. 如权利要求10所述的电化学式传感器,其特征在于该塑胶片是一种塑胶网片材质。 11. The electrochemical sensor according to claim 10, characterized in that the plastic sheet is a sheet of plastic mesh material.
  12. 12. 如权利要求IO所述的电化学式传感器,其特征在于:该第一测试电极、第二测试电极与第三测试电极可经过电极的前置处理, 该前置处理包括溶液拋光、粉体拋光、纯水超声波洗净或去离子水超声波洗净程序,以确定无吸附其他杂物的电极表面干净;该第一测试电极或第三测试电极还经过修饰作业,以形成特定用途的修饰电极;该第三测试电极以网版印刷或电解电镀以设置一个银/氯化银层,以完成该修饰作业,而形成一个氯化银参考电极;该第一测试电极还经过修饰作业而设置一层化学试剂,以形成一个工作电极;该电化学式传感器的化学试剂包含酶催化剂、酸碱值缓冲剂、界面活性剂、 氧化还原反应电子媒介物、亲水性的高分子聚合物等混合的复合物;该电化学式传感器的化学试剂中的酶具有葡萄糖氧化酶,以为测试人体的全血,而获得对 IO 12. The electrochemical sensor as claimed in claim, wherein: the first test electrode, a second test electrode and the third electrode may be subjected to pre-processing the test electrodes, the pre-processing solution comprises a polishing powder polishing, ultrasonic cleaning with pure water or deionized water ultrasonic cleaning procedures to determine the non-adsorbed other debris clean electrode surface; the first test electrode or the third electrode further modified test operations to form a particular use of the modified electrode ; the third test electrode by screen printing or electrolytic plating is provided to a silver / silver chloride layer, to complete the modification operation to form a silver chloride reference electrode; the first test electrode is also provided a modified job Chemicals layer, to form a working electrode; Chemicals the electrochemical sensor comprises an enzyme catalyst, pH buffering agents, surfactants, redox reaction electrons composite hybrid vehicle, like a hydrophilic polymer thereof; Chemicals in the electrochemical sensor having a glucose oxidase enzyme, that human whole blood test, to obtain 流体样本的分析结果,该分析结果即为该人血中的血醣浓度;该电化学式传感器的化学试剂中的酶具有尿素氧化酶,以为测试人体的全血,而分析该人血中的尿酸浓度;及/或该电化学式传感器的化学试剂中的酶具有胆固醇氧化酶,以为测试人体的全血,而分析该人血中的胆固醇浓度。 Fluid sample analysis result, the analysis result is the glucose concentration in the human blood; Chemicals in the electrochemical sensor having a urea oxidase enzymes, that human whole blood test, and analysis of the uric acid concentration in human blood ; and / or chemicals, the electrochemical sensor having an enzyme cholesterol oxidase, human whole blood that test, and analysis of the cholesterol concentration in human blood.
  13. 13. 如权利要求3所述的电化学式传感器,其特征在于:该第一贯孔及该第一测试电极各具有一个贯孔截面积及一个电极截面积, 该贯孔截面积是相同于该电极截面积,且该第一贯孔是位于该绝缘基材的底面;该第一导电条置于该绝缘基材的底面,并且该第一导电条经由第一贯孔以形成该第一测试电极,该第一测试电极即位于该绝缘基材的顶面,而该绝缘基材的顶面还具有一层第一印刷导电金属膜,以形成一个第二测试电极,而该绝缘基材的顶面还具有一层第二印刷导电金属膜,以形成一个第三测试电极,且该绝缘基材的顶面的该第二印刷导电金属膜可经氯化银的电极表面修饰以为一个氯化银参考电极;该化学试剂是与该流体样本的该成分产生电化学反应,并转化该测量信号为一个电性参数的测量值,且该电性参数的测量值相关于该成分的一种物质 13. The electrochemical sensor according to claim 3, wherein: the first through hole and each of the first test electrode having a through hole and a cross-sectional area of ​​the electrode cross-sectional area of ​​the through hole in the same cross sectional area sectional area of ​​the electrode, and the first through hole is located on the bottom surface of the insulating substrate; a first conductive strip placed on the bottom surface of the insulating substrate, and the first conductive strip via a first through hole to form the first test electrodes, i.e., the first test electrode on the top surface of the insulating substrate, and the top surface of the insulating substrate further having a layer of a first printing conductive metal film to form a second test electrode and the insulating substrate, a second top surface further includes a layer of printed conductive metal film to form a third test electrode, and the second printing conductive metal film top surface of the insulation material that can be modified by a chlorinated silver chloride electrode surface silver reference electrode; the chemical agent is an electrochemical reaction with the components of the fluid sample, and the measurement signal is converted to a measurement of electrical parameters, and measured values ​​of the electrical parameters related to the substance of the component 度;该绝缘基材是采用一种塑胶树脂,包括聚氯乙烯、聚丙烯、聚碳酸酯、聚对苯二甲酸亚丁烯基酯、聚对苯二甲酸乙二醇酯、聚氧化亚苯或丙烯腈丁二烯苯乙烯树酯;及/或该第一导电条具有一根导引线来连接该第一输出端和该第二电极端,该电极具有0.3mm-3mm的导电条厚度,该导引线具有0.2mm-2.8mm的导电条厚度。 Degrees; the insulating substrate is to use a plastic resin, including polyvinyl chloride, polypropylene, polycarbonate, polyethylene terephthalate butenylene, polyethylene terephthalate, or poly phenylene oxide acrylonitrile butadiene styrene resin; and / or the first conductive strip has a guide wire connected to the first output terminal and the second electrode terminal, the electrode has a conductive strip thickness of 0.3mm-3mm, the guide wire has a conductive strip thickness of 0.2mm-2.8mm.
  14. 14. 如权利要求13所述的电化学式传感器,其特征在于该绝缘基材是为先行成型,而于一个第一导条成型装置中埋入该绝缘基材,以形成该第一导电条。 14. The electrochemical sensor according to claim 13, wherein the insulating substrate is first formed, and a first guide on the insulating base material strip molding apparatus is embedded, to form the first conductive strip.
  15. 15. 如权利要求13所述的电化学式传感器,其特征在于该第一导电条是为先行成型,而于一个绝缘基材成型装置中埋入该第一导电条,以形成该绝缘基材。 15. The electrochemical sensor according to claim 13, wherein the first conductive strip is formed first, and the first conductive strip embedded in the insulating base in a molding apparatus to form the insulating substrate.
  16. 16. 如权利要求13所述的电化学式传感器,其特征在于该第一导电条与该绝缘基材是以双材料射出方式一次成型。 16. The electrochemical sensor according to claim 13, wherein the first conductive strip and the insulating substrate is a dual-mode Injection molding material.
  17. 17. 如权利要求13所述的电化学式传感器,其特征在于该第一导电条是利用黏胶以接合至该绝缘基材上。 17. The electrochemical sensor according to claim 13, wherein the first conductive strip to the use of viscose bonded onto the insulating substrate.
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