CN1614403A - Structure and producing process for electrochemcial sensoring testing block - Google Patents
Structure and producing process for electrochemcial sensoring testing block Download PDFInfo
- Publication number
- CN1614403A CN1614403A CN 200310114220 CN200310114220A CN1614403A CN 1614403 A CN1614403 A CN 1614403A CN 200310114220 CN200310114220 CN 200310114220 CN 200310114220 A CN200310114220 A CN 200310114220A CN 1614403 A CN1614403 A CN 1614403A
- Authority
- CN
- China
- Prior art keywords
- electrode
- electro
- reaction groove
- groove
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012360 testing method Methods 0.000 title claims description 87
- 238000000034 method Methods 0.000 title claims description 23
- 230000008569 process Effects 0.000 title description 10
- 238000006243 chemical reaction Methods 0.000 claims abstract description 77
- 238000003487 electrochemical reaction Methods 0.000 claims abstract description 60
- 229910052751 metal Inorganic materials 0.000 claims description 42
- 239000002184 metal Substances 0.000 claims description 42
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims description 31
- 239000000463 material Substances 0.000 claims description 30
- 239000000758 substrate Substances 0.000 claims description 29
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 26
- 239000003153 chemical reaction reagent Substances 0.000 claims description 25
- 238000005259 measurement Methods 0.000 claims description 25
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 24
- 230000007246 mechanism Effects 0.000 claims description 23
- 229910052802 copper Inorganic materials 0.000 claims description 20
- 239000010949 copper Substances 0.000 claims description 20
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 18
- 239000012530 fluid Substances 0.000 claims description 18
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 17
- 229910052763 palladium Inorganic materials 0.000 claims description 16
- 229910045601 alloy Inorganic materials 0.000 claims description 15
- 239000000956 alloy Substances 0.000 claims description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 14
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 13
- 229910052737 gold Inorganic materials 0.000 claims description 13
- 239000010931 gold Substances 0.000 claims description 13
- 229910052697 platinum Inorganic materials 0.000 claims description 13
- 229910052703 rhodium Inorganic materials 0.000 claims description 13
- 239000010948 rhodium Substances 0.000 claims description 13
- MHOVAHRLVXNVSD-UHFFFAOYSA-N rhodium atom Chemical compound [Rh] MHOVAHRLVXNVSD-UHFFFAOYSA-N 0.000 claims description 13
- 229910052759 nickel Inorganic materials 0.000 claims description 12
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 11
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 11
- 229910052799 carbon Inorganic materials 0.000 claims description 11
- 239000010936 titanium Substances 0.000 claims description 11
- 229910052719 titanium Inorganic materials 0.000 claims description 11
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 claims description 10
- 239000012491 analyte Substances 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 239000011148 porous material Substances 0.000 claims description 10
- 229910052707 ruthenium Inorganic materials 0.000 claims description 10
- 229910052709 silver Inorganic materials 0.000 claims description 10
- 239000004332 silver Substances 0.000 claims description 10
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 9
- 239000004411 aluminium Substances 0.000 claims description 9
- 229910052782 aluminium Inorganic materials 0.000 claims description 9
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 9
- 229910052742 iron Inorganic materials 0.000 claims description 9
- 229910052741 iridium Inorganic materials 0.000 claims description 8
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 claims description 8
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 7
- 229910052804 chromium Inorganic materials 0.000 claims description 7
- 239000011651 chromium Substances 0.000 claims description 7
- -1 polypropylene Polymers 0.000 claims description 7
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 claims description 6
- 229910001369 Brass Inorganic materials 0.000 claims description 5
- 229910000906 Bronze Inorganic materials 0.000 claims description 5
- 229910000570 Cupronickel Inorganic materials 0.000 claims description 5
- RIRXDDRGHVUXNJ-UHFFFAOYSA-N [Cu].[P] Chemical compound [Cu].[P] RIRXDDRGHVUXNJ-UHFFFAOYSA-N 0.000 claims description 5
- DMFGNRRURHSENX-UHFFFAOYSA-N beryllium copper Chemical compound [Be].[Cu] DMFGNRRURHSENX-UHFFFAOYSA-N 0.000 claims description 5
- 239000010951 brass Substances 0.000 claims description 5
- 239000010974 bronze Substances 0.000 claims description 5
- YOCUPQPZWBBYIX-UHFFFAOYSA-N copper nickel Chemical compound [Ni].[Cu] YOCUPQPZWBBYIX-UHFFFAOYSA-N 0.000 claims description 5
- KUNSUQLRTQLHQQ-UHFFFAOYSA-N copper tin Chemical compound [Cu].[Sn] KUNSUQLRTQLHQQ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 210000004276 hyalin Anatomy 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 150000002739 metals Chemical group 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 150000001879 copper Chemical class 0.000 claims description 3
- 150000002148 esters Chemical class 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 3
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 3
- 239000004800 polyvinyl chloride Substances 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 230000001143 conditioned effect Effects 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- 238000009413 insulation Methods 0.000 abstract 3
- 238000000840 electrochemical analysis Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 24
- 238000010586 diagram Methods 0.000 description 20
- 239000007772 electrode material Substances 0.000 description 9
- 238000005406 washing Methods 0.000 description 9
- 239000008280 blood Substances 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 8
- 230000003197 catalytic effect Effects 0.000 description 5
- 229910000510 noble metal Inorganic materials 0.000 description 5
- 230000027756 respiratory electron transport chain Effects 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 239000002360 explosive Substances 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 108010015776 Glucose oxidase Proteins 0.000 description 3
- 239000004366 Glucose oxidase Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229940116332 glucose oxidase Drugs 0.000 description 3
- 235000019420 glucose oxidase Nutrition 0.000 description 3
- 229910002804 graphite Inorganic materials 0.000 description 3
- 239000010439 graphite Substances 0.000 description 3
- 235000011837 pasties Nutrition 0.000 description 3
- 239000010970 precious metal Substances 0.000 description 3
- KNVAYBMMCPLDOZ-UHFFFAOYSA-N propan-2-yl 12-hydroxyoctadecanoate Chemical group CCCCCCC(O)CCCCCCCCCCC(=O)OC(C)C KNVAYBMMCPLDOZ-UHFFFAOYSA-N 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 229910000967 As alloy Inorganic materials 0.000 description 2
- 229910021607 Silver chloride Inorganic materials 0.000 description 2
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 2
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 239000004568 cement Substances 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 2
- 229940116269 uric acid Drugs 0.000 description 2
- 241001226615 Asphodelus albus Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 229910001092 metal group alloy Inorganic materials 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000001012 protector Effects 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
An electrochemical test plate consists of an insulation baseplate with at least one reaction groove formed in one body with the insulation baseplate, a side wall in width of 0.1-3.0 mm between insulation baseplate one edge and reaction groove one side, a cover with at least one air hole to cover the groove partially, an electrochemical reaction layer in the groove for carrying out reaction and an electrode device in the groove for detecting and transmitting signal generated by electrochemical reaction.
Description
Technical field
The present invention refers to a kind of creation of deserted electrochemical sensing test piece especially about the creation of an electrochemical sensing test piece.
Background technology
The present invention applies for a patent the continuity of sequence number No. 92109157 (TaiWan, China application) before being.The present invention continue the basic framework of this patent＜but changed test piece structure and manufacture method, to produce the deserted sensing test piece (Electrochemical Sensor Strip) that another kind has metal electrode (Metal Electrode), it is applicable to the content that certain analyte in the fluid measured is treated in test one, for example: the concentration of materials such as heavy metal in blood sugar, uric acid, cholesterol concentration and the sewage in the test blood and agricultural chemicals; In other words, be applicable to the detecting device (for example blood sugar test device) etc. of various electric chemical formula detecting device, biological sensor, fluid biochemical component sensor and various house medical applications by the made deserted electrochemical sensing test piece of the present invention.
The principle of electrochemical sensor (Electrochemical Sensor) was applied to by maturation in the detection of various fluids in recent decades.The equipment of an Electrochemical Detection in the control laboratory of a laboratory or specialty, because of different demands have different outfits, but its basic framework is nothing more than having: 1. one ccontaining one container that takes up for the treatment of fluid measured; 2. a chemical reagent (Reagent) is used for treating that with this one of fluid measured analyte produces an electrochemical action, and produces the output signal of an electrical parameter, and the output valve of this electrical parameter is relevant with this analyte for the treatment of fluid measured; 3. many test electrodes, it comprises electrode (Counter Electrode), working electrode (WorkingElectrode), reference electrode (Reference Electrode) and detecting electrode (DetectingElectrode); And 4. 1 measurement mechanisms, in order to necessary operating voltage to be provided, to receive this output signal and measure a measured value to show the content of this analyte.Wherein, when the blood for the treatment of that fluid measured is behaved, and analysans can utilize a glucose oxidase ferment and other compounds to be used as chemical reagent when being the blood candy so.
Again, general common blood-glucose meter can have only two test electrodes, and it is respectively for to electrode (CounterElectrode) and working electrode (Working Electrode); Or have only reference electrode (ReferenceElectrode) and working electrode.Certainly it also can use three electrodes simultaneously, promptly working electrode, to electrode outer and reference electrode; Or it also can add the detecting electrode (Detecting Electrode) that quantity does not wait, and is the electrode because of the different different numbers of arranging in pairs or groups of electrochemical action demand.
Except the difference of electrode number, because of the chemical reaction composition of the function difference (as the difference of counter electrode) of different electrodes, required sensing with respect to working electrode also different, each electrode material selected very large variation for use.The general electrode material in laboratory is as follows, wherein to electrode (CounterElectrode) material so long as the conduction material get final product, but conductive resistance is lower better,, graphite rod excellent or with carbon paste or the printed and formed conducting film electrode of elargol as copper rod, silver rod, nickel, certainly noble metals such as gold, platinum also can be used for when to utmost point electrode material, are the material cost height.Wherein the material of reference electrode (ReferenceElectrode) is the most frequently used is one silver medal/silver chloride modified electrode (Modified Electrode), because of the galvanochemistry operating potential of silver chloride highly stable, thereby by widely with being reference electrode.And selecting for use of the electrode material of working electrode (Working Electrode) is more complicated, it has two general orientation, the first kind is for utilizing the vectorial modification working electrode of electron transfer (Electron-Transfer Mediator ModifiedWorking Electrode), and second class is metal catalytic electrode (Metal-Catalyzed Electrode).Be to solidify earlier (immobilized) one deck chemical reagent on the vectorial electrode of electron transfer in the first kind wherein, this chemical reagent then contains a ferment (Enzyme such as glucose oxidase ferment Glucose oxidase) and a redox reaction electron mediator (Redox Mediator is time blood salt potassium ferricyanide as one of Mediator of being widely used in blood glucose test).When deriving (or consumption) a certain new chemical constitution behind this ferment and the test analyte generation chemical reaction when (as producing H202), utilize the electronics that is discharged in the oxidation-reduction process of this electron mediator and this new chemical constitution (for example H202) again and produce an electrical signal, and export its electrical measurement parameter via electrode.The purpose of this type of working electrode is only for be the usefulness of conduction, do not have a function of chemical catalysis, but its when making, need not select for use can with the electrode material for the treatment of fluid measured or chemical reagent generation chemical action to avoid interference measurement accuracy.Electrode material commonly used mostly is an inactive, conductive material, for example the electrode of metal such as gold, platinum, palladium, rhodium or carbon containing material (as carbon film print electrode Carbon Base ScreenPrinting Electrode, graphite rod Graphite Bar).Because of carbon and inert metal can not chemically react at low temperatures, disturb so can not produce chemistry.Noble metal cost height again so the general carbon electrodes that use of the working electrode of the modification working electrode of electron-like medium transfer thing (Electron-Transfer Mediator Modified Working Electrode) more.Metal catalytic electrode (Metal-CatalyzedElectrode) as for second class, its working electrode material of selecting for use needs can be directly and chemical reagent or analyte or derivatives thereof generation electrochemical action, so can have direct catalytic action or analyte be had the function of single selective.Just therefore, class electrode itself can cause catalytic reaction, so do not need to add electron transfer medium (Mediator) in chemical reagent again.This has the electrode material of direct catalytic action, be generally the metal electrode that contains metal ingredient, but need select different metal electrode material for use because of the determinand composition is different, the material of general normal use has copper, titanium, nickel, gold, platinum, palladium, rhodium ... on metal (wherein rhodium electrode has splendid direct catalytic action to H202).
The 5th of United States Patent (USP), 997, No. 817, it is an invention of using metal electrode, it is with two same sizes and lining＜Coating all〉one deck precious metal palladium＜Palladium bonding jumper as the metal electrode on the insulated substrate (its be applied to working electrode with on the electrode), but, because of the consumptive material of precious metal palladium bigger, and really need use precious metal palladium material characteristic person only with the contacted part of electro-chemical reaction layer (as long as remaining part can conduct electricity), so complete be a kind of meaningless waste with the whole surface of metal electrode cymbals metal reality that all is covered.
In addition, United States Patent (USP) the 5th, 985, No. 116 then is a deserted example that prints electrode, it need use the electrode by pasty state conductive paste printing moulding, about the one-tenth branch of the pasty state conductive paste that its sensing effect is then used; When with noble metal during as the pasty state conductive paste, it can have preferable susceptibility and lower chemical interference effect, but but unusual height of cost; Otherwise if the lower conductive carbon film of use cost is made electrode, though it can effectively reduce required cost, but the electrode that is obtained is because of the higher error that easily causes measuring-signal of impedance.
So (for example: the main force of the detection test piece detection test piece of blood sugar, uric acid, cholesterol), detecting that degree of accuracy and cost of manufacture but all have can improved again space though various electrochemical sensing chip has become now at home medical applications.
See also Fig. 1, be the making process flow diagram of electrochemical sensing test piece commonly used, its step comprises: 1. an insulated substrate is provided; 2. set up electrode on this insulated substrate; 3. the group structure goes out a reactive tank on this insulated substrate; 4. apply a chemical reagent to form an electro-chemical reaction layer in this reactive tank and on this electrode; 5. treat dry this reactive tank of rear enclosed of chemical reagent; 6. wash away an opening in the mode of washing away at the side of insulated substrate.
See also Fig. 2 (A)-(C), wherein Fig. 2 (A) was washing away the constitutional diagram of Cheng Qian for electrochemical sensing test piece commonly used, Fig. 2 (B) is the constitutional diagram of electrochemical sensing test piece after the process of washing away commonly used, and Fig. 2 (C) is then for to treat that fluid measured enters the synoptic diagram of electrochemical sensing test piece commonly used; Wherein should comprise insulated substrate 31, electrode 32, electro-chemical reaction layer 33, reactive tank 34, overlay 35 and pore 37 by electrification sensing test piece 3.Shown in Fig. 2 (A), this reactive tank 34 is a groove that seals all around before not washing away, after a liquid chemical reagent enters, this chemical reagent just can not flow out outside the reactive tank 34, treat promptly to generate this electro-chemical reaction layer 33 after the chemical reagent drying, wash away step process electrification sensing test piece 3 so that it has the side openings 36 shown in Fig. 2 (B) with one more at last, wherein determinand A system enters reactive tank 34 and then with this electro-chemical reaction layer 33 electro-chemical reaction takes place via this opening 36 that is positioned at electrochemical sensing test piece 3 sides, and causes a signal.When wherein handling electrochemical sensing test piece 3 in the mode of washing away, it also washes out this electro-chemical reaction layer 33 of part, wash away in mechanical stress and may cause this electro-chemical reaction layer be full of cracks 331 to occur in the process, if be full of cracks is positioned at the top of this electrode 32, then after making this determinand A enter reactive tank 34, it may promptly directly contact with this electrode 32 before not through the chemical reagents reaction of electro-chemical reaction layer 33 and the electro-chemical reaction outside the expection has taken place, and then will reduce the degree of accuracy of electrochemical sensing test piece 3.
Therefore,, cause after experiment, test and research, obtain a kind of structure and method for making of deserted electrochemical sensing test piece finally through the inventor for the cost of manufacture of the metal electrode that reduces deserted sensor test piece and bad.The present invention be except can effectively reducing cost of manufacture and simplify making the flow process, also can avoid the sensing test piece inner structure to be subjected to unnecessary destruction in manufacturing process and causes and detect the shortcoming that degree of accuracy reduces.So the applicant is through concentrated test and research in view of the disappearance of known technology, and a spirit of working with perseverance, and develops " structure of electrochemical sensing chip and the processing procedure of this case eventually.
Summary of the invention
The invention provides a kind of electrochemical sensing test piece, it comprises: an insulated substrate, it has the reaction groove of at least one periphery seal, and between an edge of a side of this reaction groove and this insulated substrate, have the sidewall that a width is 0.1～3.0mm, and this insulated substrate and this reaction groove are formed in one; One covering part, only part covers on this reaction groove, so that this reaction groove leaves an opening; One electro-chemical reaction layer, it is positioned at this reaction groove, is the position that an electro-chemical reaction takes place; And an electrode assembly, it is installed in this reaction groove, is in order to detect and to transmit the signal that this electro-chemical reaction produces.
According to above-mentioned conception, wherein an end of this electrode assembly is to link to each other so that this signal is inputed to this measurement mechanism with a measurement mechanism.
According to above-mentioned conception, this measurement mechanism is measured this signal and the result of gained is presented.
According to above-mentioned conception, wherein the other end of this electrode assembly links to each other with this electro-chemical reaction layer.
According to above-mentioned conception, wherein this reaction groove has at least one perforation.
According to above-mentioned conception, wherein this electrode assembly passes this perforation.
According to above-mentioned conception, wherein this electrode assembly comprises a plurality of electrodes.
According to above-mentioned conception, wherein these a plurality of electrode systems are selected from electrode, working electrode, reference electrode and detecting electrode.
According to above-mentioned conception, wherein these a plurality of electrodes comprise a pair of electrodes and a working electrode at least.
According to above-mentioned conception, wherein these a plurality of electrodes comprise a reference electrode and a working electrode at least.
According to above-mentioned conception, wherein these a plurality of electrodes cover the layer of metal film by a conduction material respectively and form.
According to above-mentioned conception, wherein this metallic film is made up of the goods, materials and equipments that is selected from metals such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron or aluminium and alloy thereof.
According to above-mentioned conception, wherein these a plurality of electrodes are made of a conductive base.
According to above-mentioned conception, wherein this conductive base is made of a metal or the composition that contains this metal.
According to above-mentioned conception, wherein this metal is to be selected from metal and alloys thereof such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron or aluminium.
According to above-mentioned conception, wherein this metal is to be this copper or its alloy, as brass, oxygen-free copper, bronze, phosphor-copper, foreign copper-nickel alloy, beryllium copper.
According to above-mentioned conception, wherein this electro-chemical reaction layer also comprises a chemical reagent, in order to one treat that an analyte (Analyte) composition in the fluid measured this electro-chemical reaction takes place to cause this signal.
According to above-mentioned conception, wherein this treats that fluid measured is to enter this reaction groove by this perforate.
According to above-mentioned conception, wherein have a pore at least on this emulsion sheet, in order to form a capillary flow path to discharge orifice, advance to make and treat that fluid measured is covered with fully with the pressure in the conditioned reaction groove.
According to above-mentioned conception, wherein this reaction groove system is a plastic rubber ejaculate molding with this insulated substrate.
According to above-mentioned conception, wherein this insulated substrate is made up of Polyvinylchloride, polypropylene, polycarbonate, poly terephthalic acid butenylidene ester, polyethylene terephthalate, the inferior benzene of polyoxygenated or the one of acrylonitrilebutadienestryrene resin.
According to above-mentioned conception, wherein the optimum width of this sidewall is 0.2-0.6mm.
According to above-mentioned conception, wherein the width of this opening is between 0.1-3mm, and wherein Zui Jia width is 0.2-1.2mm.
According to above-mentioned conception, wherein this covering part also has a view window.
According to above-mentioned conception, wherein this view window comprises a hyaline membrane.
The present invention provides a kind of manufacture method of electrochemical sensing test piece in addition, and it comprises the steps: to provide an insulated substrate, and it has at least one reaction groove, and this insulated substrate and this reaction groove are formed in one; Set up at least one electrode assembly and react groove in this; Form one with the contacted electro-chemical reaction layer of this electrode assembly react groove in this; Then partly cover this reaction groove with an overlay again, so that make this reaction groove have an opening that communicates with the external world.
According to above-mentioned conception, wherein this electrode assembly comprises a plurality of electrodes.
According to above-mentioned conception, wherein these a plurality of electrodes cover the layer of metal film by a conduction material respectively and form.
According to above-mentioned conception, wherein this conduction material is by a metal, a carbon or the constituent that contains these materials is constituted.
According to above-mentioned conception, wherein this metal system is selected from metal and alloys thereof such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron or aluminium.
According to above-mentioned conception, wherein this metal is this copper or its alloy, as brass, oxygen-free copper, bronze, phosphor-copper, foreign copper-nickel alloy, beryllium copper.
According to above-mentioned conception, wherein this metallic film system is made up of the goods, materials and equipments that is selected from metal such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron or aluminium and alloy thereof.
According to above-mentioned conception, wherein this electro-chemical reaction layer is that the coated chemical reagent of mat is realized in this reaction groove.
According to above-mentioned conception, wherein this electro-chemical reaction layer is that mat drips this chemical reagent and realizes in this reaction groove.
According to above-mentioned conception, wherein this electro-chemical reaction layer is that mat scatters this chemical reagent and realizes in this reaction groove.
According to above-mentioned conception, wherein this reaction groove also has at least one perforation and is connected with a measurement mechanism for this electrode assembly.
According to above-mentioned conception, wherein this covering part has at least one pore, and it is in order to regulate the pressure of this reaction groove.
For further specifying above-mentioned purpose of the present invention, design feature and effect, the present invention is described in detail below with reference to accompanying drawing.
Description of drawings
Fig. 1 is the making process flow diagram of electrochemical sensing test piece commonly used;
Fig. 2 (A) was washing away the constitutional diagram of Cheng Qian for electrochemical sensing test piece commonly used;
Fig. 2 (B) is the constitutional diagram of electrochemical sensing test piece after the process of washing away commonly used;
Fig. 2 (C) enters the synoptic diagram of electrochemical sensing test piece commonly used for determinand;
Fig. 3 is the process flow diagram that electrochemical sensing test piece of the present invention is made;
Fig. 4 (A) is the electrochemical sensing test piece explosive view of this case first preferred embodiment;
Fig. 4 (B) is the constitutional diagram of the electrochemical sensing test piece of this case first preferred embodiment;
Fig. 4 (C) is the back view of the electrochemical sensing test piece of this case first preferred embodiment;
Fig. 5 (A)-(B) detects the synoptic diagram of a test substance for the electrochemical sensing test piece of this case first preferred embodiment;
Fig. 6 (A)-(B) combines synoptic diagram for the electrochemical sensing test piece of this case first preferred embodiment with a measurement mechanism;
Fig. 7 (A) is the electrochemical sensing test piece explosive view of this case second preferred embodiment;
Fig. 7 (B) is the constitutional diagram of the electrochemical sensing test piece of this case second preferred embodiment;
Fig. 7 (C) is the back view of the electrochemical sensing test piece of this case second preferred embodiment; And
Fig. 8 (A)-(B) combines synoptic diagram for the electrochemical sensing test piece of this case second preferred embodiment with a measurement mechanism.
Embodiment
The structure of the electrochemical sensing chip of this case and method for making can fully be understood by following embodiment explanation, and be made that being familiar with present technique field person can finish it according to this, and the enforcement kenel of right this case is not restricted in the following example.
See also Fig. 3, Fig. 3 is the making process flow diagram of electrochemical sensing test piece of the present invention; Its step comprises: 1. an insulated substrate with a reaction groove is provided; 2. set up at least one electrode assembly and react groove in this; 3. form one with the contacted electro-chemical reaction layer of this electrode assembly in this reaction groove; And 4. come part to cover this reaction groove with a covering part, so that leave the opening that this reaction groove of a confession and the external world communicate.Wherein this electro-chemical reaction layer is that mat is coated, mode such as distribution or drippage applies a chemical reagent and go into this reaction groove and get; And this electrode assembly is made up of a plurality of electrodes and a plurality of metallic film.Wherein these a plurality of electrodes are made up of metal or alloy or carbon or the material of other tool electric conductivity, and common base material has: the alloy of metal such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron or aluminium or its etc.; The material of metallic film then is to be selected from copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium.In addition, the normal insulated substrate that uses is made up of Polyvinylchloride, polypropylene, polycarbonate, poly terephthalic acid butenylidene ester, polyethylene terephthalate, the inferior benzene of polyoxygenated or acrylonitrilebutadienestryrene resin.Because the present invention sees through the element that will have particular geometries to be made up on making, so successfully exempted the step of washing away commonly used, also therefore can avoid the chemical reaction layer to suffer the destruction of the stress of non-essential fully.
See also Fig. 4 (A)-(C), Fig. 4 (A) is the electrochemical sensing test piece explosive view of this case first preferred embodiment; Fig. 4 (B) is the constitutional diagram of this electrification sensing test piece; Fig. 4 (C) then is the back view of this electrification sensing test piece.Shown in Fig. 4 (A)-(C), have a reaction groove 11, the electro-chemical reaction layer 12 in this reaction groove 11, a covering part 13, an opening 14 on one insulated substrate 1, wherein this covering part 13 has a pore 131 and a view window 132, and only part hides this reaction groove 11 to leave this opening 14, in addition, between the edge of reaction groove 11 and insulated substrate 1, more there is a side 15.In addition, in reaction groove 11 bottoms is to have two perforations 111, and can run through these perforations 111 to electrode (CounterElectrode) 112 and working electrode (Working Electrode) 113, wherein the end facet to electrode 112 and working electrode 113 respectively has metallic film 1121,1122,1131 and 1132; Wherein metallic film 1121 contacts with this electro-chemical reaction layer 12 with 1131, comes from the electro-chemical reaction that takes place in the electro-chemical reaction layer 12 so that detect.Because the invention provides electrochemical sensing test piece with side 15 and opening 14, so, in manufacturing process, need not use always wash away step (be in order to produce one can for test substance enter the reaction groove 11 inlet), therefore use the integrality that the present invention can guarantee electro-chemical reaction layer 12, and and then the degree of accuracy of promoting electro-chemical reaction.In addition, reaction groove 11 and the position of covering part 13 and the enforcement kenel that shape is not limited to present embodiment.
Please refer to Fig. 5 (A)-(B), it detects the synoptic diagram of a test substance for the electrochemical sensing test piece of this case first preferred embodiment.When a test substance A enters reaction groove 11 by opening 14, this electro-chemical reaction takes place with electro-chemical reaction layer 12 interior contained chemical substance in this test substance A immediately, and pore 131 in can getting rid of reaction groove 11 portion gas and make treat that fluid measured A can be covered with fully, it also can be used to lead off the gas that is produced in this electro-chemical reaction, advance inside and outside guaranteeing reacting groove 11 to maintain under the state of air pressure balance, also can make this electro-chemical reaction in this reaction groove 11 not be subjected to non-essential influence simultaneously.Observation window 132 comprises a hyaline membrane, so that observe the generation of the mobile and electro-chemical reaction of test substance A when test substance A enters reaction groove 12.Wherein, the width b of the side 15 of present embodiment wherein is again best with 0.2-0.6mm between 0.1-3.0mm; The width a of opening 14 then is many between 0.1-3mm, wherein is again best with 0.2-1.2mm.Because the width of the side of present embodiment 15 narrow in the extreme (width is less than 3.0mm), therefore, if test substance A is a fluid material, so when test substance A contacts the edge of insulated substrate 1, its very easily directly overflow cross this side 15 and contact with opening 14, but and then regulated in the reaction groove 11 air pressure because of pore 131 and object under test A mat capillarity entered react groove 11.In addition, where necessary, can replace this to electrode 112 by a reference electrode, and other electrodes can be set more with usefulness as detecting electrode.At present often be used to make electrode or the material of metallic film comprises: metal and materials such as alloy or carbon thereof such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, silver, iron or aluminium; And serve as present more common material with brass, oxygen-free copper, bronze, phosphor-copper, foreign copper-nickel alloy, beryllium copper wherein.Again, the main benefit of doing to design with metallic film collocation electrode is effectively to reduce cost; Because the part that really need have high response and stability only is the end points of electrode assembly, so, only utilize the noble metal material, and use other metals or carbon-point can effectively reduce the cost of electrode assembly at other positions in this part.In addition, the plastic cement shooting method is one-body molded get the insulated substrate of present embodiment in order to utilize with the reaction groove, this can exempt use always test piece with layer structure the cost of required cost when superimposed.
Please refer to Fig. 6 (A)-(B), for the electrochemical sensing test piece of this case first preferred embodiment combines synoptic diagram with a measurement mechanism.Shown in Fig. 4 (A)-Fig. 4 (B), when electrochemical sensing test piece and a measurement mechanism 2 mutually in succession the time, the signal due to the chemical reagent generation electro-chemical reaction that test substance A (Fig. 5 (B)) and electro-chemical reaction layer 12 are contained promptly sees through metallic film 1122 and 1132 and transfer out the value of measuring and calculate this signal representative to measurement mechanism 2 in order to 2 pairs of these signals of measurement mechanism; In addition, this measurement mechanism 2 is to have display 21, operating key 22 and engagement device 23, and wherein this display 21 is in order to present the value of this signal representative, and this operating key 22 then is to be used for controlling this measurement mechanism 2.
See also Fig. 7 (A)-(C), Fig. 7 (A) is the electrochemical sensing test piece explosive view of this case second preferred embodiment; Fig. 7 (B) is the constitutional diagram of this electrification sensing test piece; Fig. 7 (C) then is the back view of this electrification sensing test piece.Please refer to Fig. 7 (A)-(C), wherein have a reaction groove 11, the electro-chemical reaction layer 12 in this reaction groove 11, a covering part 13 on the insulated substrate 1, have an observation window 132 and a pore 131, an opening 14 (Fig. 7 (B)) on it, wherein this covering part 13 only partly hides this reaction groove to leave this opening 14, in addition, between the edge of reaction groove 11 and insulated substrate 1, more there is a side 15.Have three perforations 111 in reaction groove 11 bottoms, electrode (Counter Electrode) 112, working electrode (Working Electrode) 113 and 114 of reference electrodes (Reference Electrode) are placed in respectively in each perforation 111, and wherein the end facet to electrode 112, working electrode 113 and reference electrode 114 respectively has metallic film 1121,1122,1131,1132,1141,1142.Metallic film 1121,1131 and 1141 is to contact with this electro-chemical reaction layer 12, comes from the electro-chemical reaction that takes place in the electro-chemical reaction layer 12 so that detect.Because the invention provides electrochemical sensing test piece with side 15 and opening 14, so, in manufacturing process, need not use always wash away step (in order to produce one can for test substance enter the reaction groove 11 inlet), therefore can guarantee the integrality of electro-chemical reaction layer 12, and and then the degree of accuracy of promoting electro-chemical reaction.In addition, the width of common side 15 wherein is again best with 0.2-0.6mm between 0.1-3.0mm; The width of opening 14 then is many between 0.1-3.0mm, wherein is again best with 0.2-1.2mm.In addition, reaction groove 11 and the position of covering part 13 and the enforcement kenel that shape is not limited to present embodiment.
Please refer to Fig. 7 (A)-(B), when a test substance enters reaction groove 11 by opening 14, this electro-chemical reaction takes place with electro-chemical reaction layer 12 interior contained chemical substance in this test substance immediately, and pore 131 in can getting rid of reaction groove 11 portion gas and make treat that fluid measured A can be covered with fully, it also can be used to lead off the gas that is produced in this electro-chemical reaction, advance inside and outside guaranteeing reacting groove 11 to maintain under the state of air pressure balance, also can make this electro-chemical reaction in this reaction groove 11 not be subjected to non-essential influence simultaneously.Observation window 132 comprises a hyaline membrane, so that observe the generation of the mobile and electro-chemical reaction of test substance when test substance enters reaction groove 12.In addition, where necessary, other electrodes can be set in addition with usefulness as detecting electrode.At present often be used to make electrode or the material of metallic film comprises: metal and materials such as alloy or carbon thereof such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, silver, iron or aluminium; And serve as present more common material with brass, oxygen-free copper, bronze, phosphor-copper, foreign copper-nickel alloy, beryllium copper wherein.Again, the main benefit of doing to design with metallic film collocation electrode is effectively to reduce cost; Because the part that really need have high response and stability only is the end points of electrode assembly, so, only utilize the noble metal material, and use other metals or carbon-point can effectively reduce the cost of electrode assembly at other positions in this part.In addition, the plastic cement shooting method is one-body molded get the insulated substrate of present embodiment in order to utilize with the reaction groove, this can exempt use always test piece with layer structure the cost of required cost when superimposed.
Please refer to Fig. 7 (A)-Fig. 8 (B), wherein Fig. 8 (A)-(B) combines synoptic diagram for the electrochemical sensing test piece of this case second preferred embodiment with a measurement mechanism.Shown in Fig. 7 (A)-Fig. 8 (B), when electrochemical sensing test piece and a measurement mechanism 2 mutually in succession the time, the signal that the chemical reagent generation electro-chemical reaction that test substance and electro-chemical reaction layer 12 contained is produced promptly sees through metallic film 1122,1132 and 1142 and transfer out the value of measuring and calculate this signal representative to measurement mechanism 2 in order to 2 pairs of these signals of measurement mechanism; In addition, this measurement mechanism 2 has display 21, operating key 22 and engagement device 23, and wherein this display 21 is in order to present the value of this signal representative, and this operating key 22 then is to be used for controlling this measurement mechanism 2.
In sum, the present invention sees through the element will have the special geometric design to be made up and exempted the step of washing away commonly used, and it can effectively be avoided the sensing test piece inner structure to be subjected to unnecessary destruction in manufacturing process and cause and detect the shortcoming that degree of accuracy reduces; Therefore what is more, because the present invention washes away step because of exempting, no matter be in the making complexity of sensing test piece or on cost, all will significantly reduce.So, can be executed that the craftsman thinks and be to modify right neither taking off as all by knowing usually that at this field tool the knowledgeable appoints even if this case has been described in detail by the above embodiments as Protector that attached claim is desired.
Though the present invention describes with reference to current specific embodiment, but those of ordinary skill in the art will be appreciated that, above embodiment is used for illustrating the present invention, under the situation that does not break away from spirit of the present invention, also can make the variation and the modification of various equivalences, therefore, as long as variation, the modification to the foregoing description all will drop in the scope of claims of the present invention in connotation scope of the present invention.
Claims (10)
1. electrochemical sensing test piece, it comprises:
One insulated substrate, it has the reaction groove of at least one periphery seal, and has the sidewall that a width is 0.1～3.0mm between an edge of a side of this reaction groove and this insulated substrate, and this insulated substrate and this reaction groove are formed in one;
One covering part, only part covers on this reaction groove, so that this reaction groove leaves an opening;
One electro-chemical reaction layer, it is positioned at this reaction groove, is the position that takes place for an electro-chemical reaction; And
One electrode assembly, it is installed in this reaction groove, in order to detect and to transmit the signal that this electro-chemical reaction produces.
2. sensing test piece as claimed in claim 1 is characterized in that,
One end of this electrode assembly links to each other with a measurement mechanism so that this signal is inputed to this measurement mechanism;
This measurement mechanism is measured this signal and the result of gained is presented; And/or
The other end of this electrode assembly is to link to each other with this electro-chemical reaction layer.
3. sensing test piece as claimed in claim 1 is characterized in that,
This reaction groove has at least one perforation; And/or
This electrode assembly passes this perforation.
4. sensing test piece as claimed in claim 1 is characterized in that,
This electrode assembly comprises a plurality of electrodes;
These a plurality of electrodes are selected from electrode, working electrode, reference electrode and detecting electrode;
These a plurality of electrodes comprise a pair of electrodes and a working electrode at least;
These a plurality of electrodes comprise a reference electrode and a working electrode at least;
These a plurality of electrodes cover the layer of metal film by a conduction material respectively and form;
This metallic film is made up of the goods, materials and equipments that is selected from metals such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron or aluminium and alloy thereof;
These a plurality of electrodes are made of a conductive base;
This conductive base is made of a metal or the composition that contains this metal;
This metal is selected from metal and alloys thereof such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron or aluminium; And/or
This metal is this copper or its alloy, as brass, oxygen-free copper, bronze, phosphor-copper, foreign copper-nickel alloy, beryllium copper.
5. sensing test piece as claimed in claim 1 is characterized in that,
This electro-chemical reaction layer also comprises a chemical reagent, in order to one treat that the analyte component in the fluid measured this electro-chemical reaction takes place to cause this signal;
This treats that fluid measured is to enter this reaction groove by this perforate; And/or
At least have a pore on this emulsion sheet, in order to form a capillary flow path to discharge orifice, advance to make and treat that fluid measured is covered with fully with the pressure in the conditioned reaction groove.
6. sensing test piece as claimed in claim 1 is characterized in that,
This reaction groove and this insulated substrate are plastic rubber ejaculate molding;
This insulated substrate is made up of Polyvinylchloride, polypropylene, polycarbonate, poly terephthalic acid butenylidene ester, polyethylene terephthalate, the inferior benzene of polyoxygenated or the one of acrylonitrilebutadienestryrene resin;
The optimum width of this sidewall is 0.2-0.6mm;
The width of this opening is between 0.1-3mm, and wherein Zui Jia width is 0.2-1.2mm;
This covering part also has a view window; And/or
This view window comprises a hyaline membrane.
7. the manufacture method of an electrochemical sensing test piece, it comprises the steps:
One insulated substrate is provided, and it has at least one reaction groove, and this insulated substrate and this reaction groove are formed in one;
Set up at least one electrode assembly and react groove in this;
Form one with the contacted electro-chemical reaction layer of this electrode assembly react groove in this; And
Partly cover this reaction groove with an overlay, so that make this reaction groove have an opening that communicates with the external world.
8. method as claimed in claim 7 is characterized in that,
This electrode assembly comprises a plurality of electrodes;
These a plurality of electrodes cover the layer of metal film by a conduction material respectively and form; And/or
This conduction material is by a metal, a carbon or the constituent that contains these materials is constituted.
9. method as claimed in claim 7 is characterized in that,
The coated chemical reagent of this electro-chemical reaction layer mat is realized in this reaction groove; And/or
This electro-chemical reaction layer mat drips this chemical reagent to be realized in this reaction groove.
10. method as claimed in claim 7 is characterized in that,
This electro-chemical reaction layer mat scatters this chemical reagent and realizes in this reaction groove;
This reaction groove also has at least one perforation and is connected with a measurement mechanism for this electrode assembly; And/or
This covering part has at least one pore, and it is in order to regulate the pressure of this reaction groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101142209A CN100392389C (en) | 2003-11-07 | 2003-11-07 | Structure and producing process for electrochemcial sensoring testing block |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101142209A CN100392389C (en) | 2003-11-07 | 2003-11-07 | Structure and producing process for electrochemcial sensoring testing block |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1614403A true CN1614403A (en) | 2005-05-11 |
| CN100392389C CN100392389C (en) | 2008-06-04 |
Family
ID=34760156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101142209A Expired - Lifetime CN100392389C (en) | 2003-11-07 | 2003-11-07 | Structure and producing process for electrochemcial sensoring testing block |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100392389C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101482537A (en) * | 2008-01-08 | 2009-07-15 | 拜尔特生物科技股份有限公司 | Hydrogen peroxide detection method |
| CN101078719B (en) * | 2006-05-24 | 2013-02-20 | 华广生技股份有限公司 | Method for operating measuring meter and measuring meter |
| CN101520428B (en) * | 2008-02-25 | 2013-05-01 | 华广生技股份有限公司 | Electrochemical formula sensing method and test piece |
| CN103201619A (en) * | 2010-11-09 | 2013-07-10 | 3M创新有限公司 | Electrochemical biosensor electrode strip and a fabrication method thereof comprising a titanium metal layer on a carbon layer as the electrode material |
| CN103412026A (en) * | 2013-08-30 | 2013-11-27 | 江苏鱼跃医疗设备股份有限公司 | Biosensor and manufacturing method thereof |
| CN103969312A (en) * | 2013-01-28 | 2014-08-06 | 红电医学科技股份有限公司 | Detection device and detection method of detection test piece |
| CN104749229A (en) * | 2013-12-26 | 2015-07-01 | 绿点高新科技股份有限公司 | Electrochemical sensing test piece, manufacturing method thereof and manufacturing method of electrode layer thereof |
| CN105259221A (en) * | 2014-07-17 | 2016-01-20 | 光宏精密股份有限公司 | Electric conduction member arrangement method of electrochemical sensing test sheet |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5646800A (en) * | 1999-03-02 | 2000-09-21 | Helix Biopharma Corporation | Card-based biosensor device |
| US6740215B1 (en) * | 1999-11-16 | 2004-05-25 | Matsushita Electric Industrial Co., Ltd. | Biosensor |
| CN1498344A (en) * | 2001-11-14 | 2004-05-19 | 松下电器产业株式会社 | Biosensor |
| CN100432660C (en) * | 2003-05-07 | 2008-11-12 | 黄椿木 | Structure of electrochemical sensing test block and producing method thereof |
| CN2793524Y (en) * | 2004-12-23 | 2006-07-05 | 北京源德生物医学工程有限公司 | Standard light source for single-photon counter |
-
2003
- 2003-11-07 CN CNB2003101142209A patent/CN100392389C/en not_active Expired - Lifetime
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078719B (en) * | 2006-05-24 | 2013-02-20 | 华广生技股份有限公司 | Method for operating measuring meter and measuring meter |
| CN101482537A (en) * | 2008-01-08 | 2009-07-15 | 拜尔特生物科技股份有限公司 | Hydrogen peroxide detection method |
| CN101520428B (en) * | 2008-02-25 | 2013-05-01 | 华广生技股份有限公司 | Electrochemical formula sensing method and test piece |
| CN103201619A (en) * | 2010-11-09 | 2013-07-10 | 3M创新有限公司 | Electrochemical biosensor electrode strip and a fabrication method thereof comprising a titanium metal layer on a carbon layer as the electrode material |
| CN103969312A (en) * | 2013-01-28 | 2014-08-06 | 红电医学科技股份有限公司 | Detection device and detection method of detection test piece |
| CN103412026A (en) * | 2013-08-30 | 2013-11-27 | 江苏鱼跃医疗设备股份有限公司 | Biosensor and manufacturing method thereof |
| CN104749229A (en) * | 2013-12-26 | 2015-07-01 | 绿点高新科技股份有限公司 | Electrochemical sensing test piece, manufacturing method thereof and manufacturing method of electrode layer thereof |
| CN104749229B (en) * | 2013-12-26 | 2019-01-29 | 绿点高新科技股份有限公司 | Electrochemical sensing test piece, manufacturing method thereof and manufacturing method of electrode layer thereof |
| CN105259221A (en) * | 2014-07-17 | 2016-01-20 | 光宏精密股份有限公司 | Electric conduction member arrangement method of electrochemical sensing test sheet |
| WO2016008334A1 (en) * | 2014-07-17 | 2016-01-21 | 光宏精密股份有限公司 | Method for arranging conductive component of electrochemical sensing test piece |
| CN105259221B (en) * | 2014-07-17 | 2017-09-22 | 光宏精密股份有限公司 | The electric-conductor method to set up of electrochemical sensing test piece |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100392389C (en) | 2008-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Moya et al. | Inkjet-printed electrochemical sensors | |
| Wang et al. | Microfabricated electrophoresis chips for simultaneous bioassays of glucose, uric acid, ascorbic acid, and acetaminophen | |
| CN1205474C (en) | Biosensor | |
| KR100340174B1 (en) | Electrochemical Biosensor Test Strip, Fabrication Method Thereof and Electrochemical Biosensor | |
| US8354012B2 (en) | Electrochemical cell | |
| AU2002367214B2 (en) | Micro-band electrode | |
| US8900440B2 (en) | Method for detecting chemical or biological species and electrode arrangement therefor | |
| CN113125537B (en) | Wearable sweat monitoring sensor and preparation method thereof | |
| Oh et al. | Recent developments in electrochemical paper-based analytical devices | |
| US7556724B2 (en) | Electrochemical sensor strip and manufacturing method thereof | |
| CN1928541A (en) | Electrochemical cell | |
| CN101454667B (en) | Electrochemical sensor system using a substrate with at least one aperature and method of making the same | |
| KR20100117173A (en) | Electrochemical biosensor electrode strip and preparing method thereof | |
| WO2005122296A2 (en) | An electrode device, process for making an electrode device and a method of electrochemical detection using an electrode device | |
| CN100504373C (en) | Electrochemical sensing chip and its production | |
| CN101520428B (en) | Electrochemical formula sensing method and test piece | |
| Deroco et al. | based electrochemical sensing devices | |
| CN1614403A (en) | Structure and producing process for electrochemcial sensoring testing block | |
| CN103201619A (en) | Electrochemical biosensor electrode strip and a fabrication method thereof comprising a titanium metal layer on a carbon layer as the electrode material | |
| EP3708360A1 (en) | Printed electrodes on cork substrates and process for obtaining them | |
| CN100342230C (en) | Structure of electrochemical sensing sheet and its producing method | |
| JP4611889B2 (en) | Disposable electrochemical sensor strip structure and manufacturing method thereof | |
| KR100805913B1 (en) | Structure and manufacturing method of disposable electrochemical sensor strip | |
| WO2006085876A1 (en) | Electrochemical sensor strip and manufacturing method thereof | |
| CN103983671B (en) | Chemical sensor and method for manufacturing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20080604 |