CN1303078C - Process for preparing effective constituent in tea - Google Patents

Process for preparing effective constituent in tea Download PDF

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Publication number
CN1303078C
CN1303078C CNB2003101146943A CN200310114694A CN1303078C CN 1303078 C CN1303078 C CN 1303078C CN B2003101146943 A CNB2003101146943 A CN B2003101146943A CN 200310114694 A CN200310114694 A CN 200310114694A CN 1303078 C CN1303078 C CN 1303078C
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China
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tea
theanine
present
technology according
xanthine
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CNB2003101146943A
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CN1634913A (en
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汪东风
王常红
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Ocean University of China
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Ocean University of China
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Abstract

The present invention discloses a process for preparing effective constituents in tea. The process extracts four effective constituents of theobromine, tea polyphenol, tea polysaccharide and theanine from the tea according to the physicochemical properties of the tea polyphenol, the theobromine, the tea polysaccharide and the theanine by using a reagent multiple order type step-by-step extraction method. The present invention overcomes the defects that a prior precipitation method has serious pollution of three kinds of waste; a CO2 supercritical extraction method has large investment of equipment; other individual component extracting method can not effectively use natural resources, etc. Additionally, the present invention also has the advantages of environment pollution by waste gas and waste water in a production process, and is a green protection type high and new technology. The product of the present invention can be widely used for the field of various kinds of food, medicinal health protection products, the production of daily chemical products, etc. The development and the use of the present invention not only can solve the outlet of the tea of middle grade and low grade and tea side products, but also are the requirements of the technical change of a plurality of tea polyphenol plants at present.

Description

Effective constituents in tea preparation technology
Technical field the present invention relates to a kind of effective constituents in tea preparation technology.
The consumption of the present tealeaves of background technology is superior day by day, and that low and middle-grade tea is subjected to the world market to influence normal deadstock is serious, has restricted the tealeaves development.Meanwhile, in the Tea Production and the course of processing, also have a large amount of trimming leafs and processed side product, do not made full use of as refuse and these products are many.
As everyone knows, tealeaves has many health-care effects, and being rich in effective constituents such as tea-polyphenol, trimethyl-xanthine, tea polysaccharide and theanine in this and the tealeaves has substantial connection.Tea-polyphenol is not only a kind of natural food antioxidant (GB12493-90), but also is the raw materials for production of plurality kinds of health care product and medicine; Trimethyl-xanthine is the additive of multiple beverage, pharmaceutically also is being widely used.The contriver is from 1991, gone out tea polysaccharide with refabrication in the waste liquid that has prepared tea-polyphenol and trimethyl-xanthine, effects such as with the experimentation on animals discovery that this product innovation carries out, that tea polysaccharide has is hypoglycemic, blood fat and enhancing body immunizing power; Seeking in the natural product research of anti-SARS recently, U.S. scientist finds that theanine can make human body resist 5 times of the ability enhancings of virus infection, is the novel medicine of potential novel antiviral, bacterium and fungi and the composition of healthcare products.This shows, these effective constituents are prepared and are used, be not only one of effective way that solves low and middle-grade tea and tea byproduct outlet, and can produce great economic benefit and social benefit.A lot of to the manufacturer of tea-polyphenol, trimethyl-xanthine extraction at present, but tea polysaccharide and theanine all fail to be prepared utilization as refuse.
Summary of the invention the purpose of this invention is to provide a kind of effective constituents in tea preparation technology, and it can extract trimethyl-xanthine, tea-polyphenol, tea polysaccharide and 4 kinds of effective constituents of theanine by substep from tealeaves.
This technology utilizes reagent multiple stage type substep extraction method to extract trimethyl-xanthine, tea-polyphenol, tea polysaccharide and 4 kinds of effective constituents of theanine from tealeaves according to the physico-chemical property of tea-polyphenol, trimethyl-xanthine, tea polysaccharide and theanine.Its preparation technology may further comprise the steps:
(1) tealeaves is pulverized back hot water lixiviate;
(2) after the cooling, the centrifugal impurity of removing;
(3) with trichloromethane or dichloromethane extraction trimethyl-xanthine;
(4) water behind the extraction trimethyl-xanthine is used ethyl acetate extraction;
(5) will be dissolved in the acidic aqueous solution the dry tea-polyphenol that gets after the ethyl acetate recovery;
(6) extract water behind the tea-polyphenol concentrate the back with reagent precipitate tea polysaccharide;
(7) supernatant liquor that extracts after the tea polysaccharide adds precipitation agent, after centrifugal theanine, through desalination and periodic crystallisation, can obtain the theanine elaboration.
In above-mentioned processing step, the hot water temperature who is used for lixiviate tealeaves should be more than 70 ℃, and weight is 100~350 times of tealeaves, and extraction time is 30~360min;
Be meant that with reagent precipitation the water after concentrating adds ethanol, acetone, ether or ethyl acetate described in the step (6);
Precipitation agent described in the step (7) is ventilation breather, magnesium basic carbonate, zinc subcarbonate, basic carbonate calcium, basic carbonate barium or basic carbonate manganese;
The desalination of theanine and periodic crystallisation are meant earlier and dissolve with dilute sulphuric acid, remove ionizable metal salt with hydrogen sulfide again, and the supernatant concentration drying with using the dehydrated alcohol periodic crystallisation several times after the small amount of thermal water dissolution, can obtain the theanine elaboration again.
This shows that technical characterstic of the present invention is: utilize reagent multiple stage type substep extraction method from tealeaves, to extract trimethyl-xanthine, tea-polyphenol, tea polysaccharide and 4 kinds of effective constituents of theanine.It is serious that it has overcome previous precipitator method three-waste pollution; CO 2The supercritical extraction facility investment is big, technological operation is difficult and other single components extract defectives such as natural resources can not effectively utilize.In addition, the present invention also has production process not have waste residue, waste gas, contaminated wastewater environment, is a kind of environmental type new and high technology.
Product tea-polyphenol of the present invention, trimethyl-xanthine, tea polysaccharide and theanine can be widely used in fields such as varieties of food items, Medicines and Health Product, makeup, household chemicals production.The exploitation of this invention is used, and will drive the development of related industries and the adjustment of the structure of rural undertaking, multiplies large quantities of product innovations.
Description of drawings Fig. 1 is the schema of production technique of the present invention.
Further specify the present invention below in conjunction with accompanying drawing and by concrete embodiment.
Embodiment with low and middle-grade tea (tankage that comprise trimming leaf and tea finish machine) after being crushed to 10 orders, with the hot water more than 70 ℃ (solid-to-liquid ratio is 1: 200) lixiviate secondary, each 120min, cooling fast, centrifugal removal of impurities, with trichloromethane or dichloromethane extraction trimethyl-xanthine, concentrating under reduced pressure also reclaims organic solvent, the thick trimethyl-xanthine of gained extracts 2 times or utilizes the method refining and edulcoration of trimethyl-xanthine distillation repeatedly with trichloromethane or methylene dichloride, can get the trimethyl-xanthine elaboration.Water behind the extraction trimethyl-xanthine is used ethyl acetate extraction, and organic phase concentrates and reclaims solvent, and crude product is dissolved in the acidic aqueous solution after drying and gets the tea-polyphenol product.Water behind the extraction tea-polyphenol concentrates the back and adds ethanol sedimentation, gets the tea polysaccharide crude product, can get the tea polysaccharide elaboration again after ethanol, acetone, ether or ethyl acetate are alternately taken off ester and employing proteolytic enzyme or chloroform amylalcohol (4: 1) deproteinization method repeatedly several times.Supernatant liquor after the extraction tea polysaccharide adds the ventilation breather precipitation agent, can get the crude product of theanine after centrifugal, the crude product of theanine dissolves with dilute sulphuric acid earlier, remove ionizable metal salt with hydrogen sulfide again, the supernatant concentration drying is again with getting the theanine elaboration several times with the dehydrated alcohol periodic crystallisation after the small amount of thermal water dissolution.

Claims (8)

1, effective constituents in tea preparation technology is characterized in that this production technique may further comprise the steps:
(1) tealeaves is pulverized back hot water lixiviate;
(2) after the cooling, the centrifugal impurity of removing;
(3) with trichloromethane or dichloromethane extraction trimethyl-xanthine;
(4) water behind the extraction trimethyl-xanthine is used ethyl acetate extraction;
(5) product after the recovery ethyl acetate is dissolved in the acidic aqueous solution, the dry tea-polyphenol that gets;
(6) will extract water behind the tea-polyphenol concentrate the back with reagent precipitate tea polysaccharide;
(7) supernatant liquor after extracting tea polysaccharide adds precipitation agent, after centrifugal theanine.
2, technology according to claim 1 is characterized in that the trimethyl-xanthine of gained obtains the trimethyl-xanthine elaboration after distillation.
3, technology according to claim 1 is characterized in that the tea polysaccharide of gained obtains the tea polysaccharide elaboration after ester and deproteinization are taken off in decolouring.
4, technology according to claim 1 is characterized in that the theanine of gained obtains the theanine elaboration behind desalination and periodic crystallisation.
5, technology according to claim 1 is characterized in that the wherein said hot water temperature who is used for lixiviate tealeaves should be more than 70 ℃, and weight is 100~350 times of tealeaves, and extraction time is 30~360min.
6, technology according to claim 1 is characterized in that wherein saidly precipitating to such an extent that tea polysaccharide is meant that the water after concentrating adds ethanol, acetone, ether or ethyl acetate with reagent.
7, technology according to claim 1 is characterized in that wherein said precipitation agent is ventilation breather, magnesium basic carbonate, zinc subcarbonate, basic carbonate calcium, basic carbonate barium or basic carbonate manganese.
8, technology according to claim 4, the desalination and the periodic crystallisation that it is characterized in that theanine are meant that elder generation with the dilute sulphuric acid dissolving, removes ionizable metal salt, supernatant concentration drying with hydrogen sulfide again, with using the dehydrated alcohol periodic crystallisation several times after the small amount of thermal water dissolution, can obtain the theanine elaboration again.
CNB2003101146943A 2003-12-31 2003-12-31 Process for preparing effective constituent in tea Expired - Fee Related CN1303078C (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105646428A (en) * 2015-12-18 2016-06-08 安徽红星药业股份有限公司 Tea polyphenol production process

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101113147B (en) * 2007-06-26 2011-08-24 朱江 Method for tea by-product resource utilization
CN101285081B (en) * 2008-04-08 2011-12-21 江苏德和生物科技有限公司 New process for preparing theaflavine
CN101798355B (en) * 2010-04-08 2015-02-11 晋江市恒源科技开发有限公司 Method for integrated separation and extraction of tea polysaccharide, theanine, polyphenol and caffeine
CN103242314B (en) * 2013-04-19 2015-06-24 天津大学 Method for comprehensively extracting effective ingredients in tea
CN105285228A (en) * 2015-11-12 2016-02-03 云南茶农生物产业有限责任公司 Preparation method for extract rich in tea polyphenol, tea polysaccharide, theanine and caffeine
CN106065022A (en) * 2016-06-07 2016-11-02 江西富之源生物科技有限公司 A kind of high-efficient production technology of tea polyphenols
CN106977517B (en) * 2017-03-21 2019-08-27 浙江大学 Substep obtains the method for being enriched the tea extract of caffeine, EGC and EGCG
CN107279369A (en) * 2017-06-07 2017-10-24 安徽农业大学 A kind of preparation method of hypoglycemic yellow tea
CN108210379A (en) * 2017-12-06 2018-06-29 芜湖市欣然食品有限公司 A kind of preparation method of Ilex Latifolia Thunb ginkgo complex emulsions
CN110269114A (en) * 2019-08-06 2019-09-24 国初科技(厦门)有限公司 A kind of novel tea juice method for concentration

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1082327A (en) * 1993-03-01 1994-02-23 茹培凌 Method to tealeaves comprehensive process and utilization
CN1161321A (en) * 1996-09-28 1997-10-08 浙江大学 Method for extracting tea polyphenol and by-products from tea leaves

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1082327A (en) * 1993-03-01 1994-02-23 茹培凌 Method to tealeaves comprehensive process and utilization
CN1161321A (en) * 1996-09-28 1997-10-08 浙江大学 Method for extracting tea polyphenol and by-products from tea leaves

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105646428A (en) * 2015-12-18 2016-06-08 安徽红星药业股份有限公司 Tea polyphenol production process

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