CN1302862A - Bioreactor for liquid culture of microbes - Google Patents
Bioreactor for liquid culture of microbes Download PDFInfo
- Publication number
- CN1302862A CN1302862A CN 99121894 CN99121894A CN1302862A CN 1302862 A CN1302862 A CN 1302862A CN 99121894 CN99121894 CN 99121894 CN 99121894 A CN99121894 A CN 99121894A CN 1302862 A CN1302862 A CN 1302862A
- Authority
- CN
- China
- Prior art keywords
- reactor
- liquid
- inoculation
- wall
- bio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
- C12M29/08—Air lift
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A bioreactor for the liquid culture of great mycelial fungus is a novel airlift bioreactor (ALR/ff) with low reflux region and particular parameters, which is composed of liquid culture reactor for seed hyphae, liquid fermentation reactor, pulse stream wall cleaner and dual-point inoculation system. Its advantages are no damage to hyphae, no deposit of colony on wall, and volume-variable material-supplementing liquid culture.
Description
The present invention relates to the microorganism fermentation process apparatus field, specifically the present invention relates to a kind of new airlift reactor with low recirculating zone.Have following characteristics: liquid stream downcomer Ad and ascent stage Ar ratio in the reactor, promptly the Ad/Ar value is in the 1.00-2.00 scope, prevents the mycelium fibre beakage, forms bacterium floc sedimentation knot wall.Changing " inner draft tube height with height for reactor h/H ratio " is≤0.50, helps mycelial normal growth.This gas lift reactor includes one or more layers stream of pulses wall cleaner and two point formula inoculation system.
Many in filamentous fungus and the large-scale filamentous fungus all is industrial microorganism or medicinal fungus, and the preparation of its cell and meta-bolites thereof and production have obvious social and considerable economic.Particularly in recent years both at home and abroad expert Physiology and biochemistry, pharmacology and clinical study result that medicinal large-scale filamentous fungal cells and product thereof are carried out shown that technical scale develops the necessity of this class medicinal fungus, thereby but on sporophore cultivation basis the deep liquid culture process of formation implement scale production just progressively.Bio-reactor in filamentous fungus liquid culture (the particularly large-scale filamentous fungus cultivate) technology (comprise kind of liquid cultivate a reactor and a liquid fermenting reactor) is continued to use traditional mechanical agitating fermentation tank of non-filamentous microorganism (for example bacterium, actinomycetes etc.) cultivation at present more.Because turbulent shear field and the bubble of carrying generation because of the trailing vortex air pocket of fluid top layer vortex and serious bubble secretly are in violent and frequent dispersion, coalescence of paddle district generation and process such as break in (particularly when higher rotation speed) liquid that agitator produces, produce fluid shearing and make very thin mycelia breakage and the influence growth, thereby seriously reduce cell cultures efficient.
Airlift circulating reactor is to form the fluid circulation and reach material transfer, oxygen transmission and heat transmission by gas injection.Because its mixed performance is good, oxygen delivery capacity is strong, and the flow field shearing force is lower, and avoids the detrimentally affect (these advantages also are that simple bubbling style reactor is incomparable) of cell growth.
The present invention cultivates for solving microbial liquid, particularly large-scale filamentous fungus (MacroFilamentous Fungi) mycelia cracky during liquid culture, cenobium knot wall and difficult problem of unfavorable growth in stirred reactor, growth characteristic at filamentous fungus, initiative ground specialized designs different with existing gas lift reactor special-purpose air-lift loop reactors (Air Lift Reactor for Filamentous fungi Cultivation is called for short ALR/ff) with ad hoc structure parameter such as low recirculating zone and other specific aim structures.The ALR/ff primary structure is characterized as:
1) () air-lift loop reactor for example, many bacteriums, actinomycetes is mainly considered to strengthen the main body turbulent flow, obtain higher oxygen transfer efficiency k to be used for obvious aerobic, non-filamentous microorganism
LThe a value.One of its key parameter " liquid stream downcomer is normal with ascent stage area ratio Ad/Ar value to be 0.80-0.90, sometimes even be 0.10-0.40.And the particularly large-scale filamentous fungus of many filamentous funguss has the fine long cracky of not intensive oxygen requirement, many cells mycelium, easily is the growth characteristic that bacterium floc sedimentation knot wall is grown.The Ad/Ar parameter designing value of ALR/ff special bio reactor is 1.00-2.00 (Fig. 1), plants the desirable high value of liquid mycelium culture reactor.This design to be avoiding causing too small turbulent vortices yardstick and multidirectional the shearing in cell surface under the strong main body field of turbulent flow, and prevents that narrow downcomer cross section from increasing the knot wall rate of hypha body.
2) in the above-mentioned general air-lift loop structure of reactor, two of its key parameter, the ratio h/H of height for reactor " the inner draft tube height with " parameter value are usually＞0.60.ALR/ff special bio reactor design is low recirculating zone/high disengaging zone, i.e. h/H parameter value≤0.50 (0.30-0.50), and kind liquid mycelium culture reactor is desirable than low value.The high disengaging zone of this design helps lowering the flow velocity of bubble to be separated (being that bubble rises to liquid stream main body and the reactor head airflow space interface place segregation that bursts apart), and increase bubble scale, the breakage (air bubble liquid-membrane adsorbs more mycelium) of mycelia when reducing bubble and burst apart.It is low that the recirculating zone/high disengaging zone makes cultivation results show as mycelial growth is evenly neat.In addition, higher disengaging zone can allow the part filamentous fungus, and particularly large-scale filamentous fungus carries out the fed batch cultivation process of cumulative volume.Because (the feed liquid height is respectively h when establishing feed supplement process beginning, final volume can to guarantee to keep from start to finish in the feed supplement process the inside and outside liquid circulation of guide shell
1, h
2, make h
1＞h
2, mending the volume height difference is (h
2-h
1)).
3) the particularly large-scale filamentous fungus process of growth of filamentous fungus bacterium floc sedimentation knot wall not only reduces culture efficiency (Productivity), and hinders mass transfer, heat transfer process, and influences the mycelia morphology Control, and is more close to kind of the relation of liquid mycelium culture especially.Further also the enforcement microcomputer is carried out process automatic control work and increased trouble.This is a problem that perplexs technology for a long time.Cenobium knot wall mainly occurs in liquid level position.ALR/ff bio-reactor design of the present invention has one or more layers stream of pulses wall cleaner (Fig. 2).Make reactor eliminate knot wall phenomenon, can also assist to solve recurrent feed liquid dehydration thickening problem in the gas lift reactor.This device is novel, simple in structure, cheap, the clear wall effect of investment is obvious.
4) bottle that shakes of bio-reactor commonly used is planted liquid inoculation operation pressure differential method commonly used or flame hole tipping.The latter is difficult to operation can not guarantee that again process is aseptic.The former can not unobstructedly inoculate for the filamentous fungus floc sedimentation shape kind liquid of large-scale filamentous fungus particularly, and has to strengthen pressure reduction or increase the pressure reduction number of times, and complex operation is dangerous.The ALR/ff bio-reactor improves inoculation method, and this reactor head design has " two point formula inoculation system ".The cotton-shaped inoculation liquid of bacterium can be under equilibrium pressure be driven and in the natural inflow reactor by liquid level difference.Whole seeded process is simple, convenient, safety, effective.
5) the ALR/ff bioreactor construction is applicable to filamentous fungus particularly kind liquid mycelium culture process and the liquid fermenting process of large-scale filamentous fungus.Attached: Fig. 1, ALR/ff bio-reactor primary structure figure; Fig. 2, the stream of pulses wall cleaner structure iron of ALR/ff bio-reactor.
Realize that concrete steps of the present invention are as follows:
The ALR/ff bio-reactor, available withstand voltage material is made, 130 ℃ of maximum operating temperatures, maximum working pressure 1.5kg/cm
2Primary structure is asked for an interview Fig. 1.Wherein Ad/Ar parameter designing value is 1.00-2.00, plants the desirable high value of liquid mycelium culture reactor; H/H parameter value≤0.50 (0.30-0.50) is planted liquid mycelium culture reactor and should be got than low value.The blade diameter length ratio D/H value of reactor gets 1: 4.0-5.0.Above parameter value can be used for laboratory mini-reactor design and is amplified to production-scale reactor.In addition, as use stainless steel, reactor adds man-hour, and weld seam must welding by both sides and polishing.Reactor wall has the smooth finish requirement.
The structure of the ring-type tubular type stream of pulses wall cleaner of ALR/ff bio-reactor is seen Fig. 2.Its constitutional features is the side of endless tube with respect to reactor wall, has equally distributed φ 3mm hole, be distributed on the endless tube, the hole towards, form 45 down towards reactor wall, make by stainless steel.One or more layers.Adopt adjustable timing valve according to growth and knot wall situation, spray the germ-free air flow or the aseptic current of certain pressure by the pulse wave cycle of setting to easy knot wall position.When using aseptic current, can assist again and solve recurrent feed liquid dehydration thickening problem in the gas lift reactor.
The ALR/ff bio-reactor is equipped with " two point formula inoculation system ", and structure is seen Fig. 3.The top and bottom that the special-purpose inoculator of stainless steel for the treatment of inoculation liquid is housed are connected with pressure hose respectively and insert pin (special, pin hole Φ 6mm), by aseptic technique, distinguish in the insertion reaction device during inoculation.Inoculation mouthful by interchangeable, tightly press, withstand voltage high-temperature resistant elastic rubber pad sealed.Nature under inoculation liquid is driven by liquid level difference under equilibrium pressure, reposefully in the inflow reactor.Above-mentioned special-purpose inoculator is made by stainless steel, the withstand voltage 2kg/cm that is not less than
2, being cylindrical structural, post jamb one side has withstand voltage glass visor, is used for metering and observes the inner liquid situation of planting.Right cylinder top and lower end are connected with above-mentioned pressure hose respectively and insert pin, and its top ports communicates with planting liquid top air, and lower port is connected to controlled clip or valve and kind liquid phase and connects.
According to the description of the invention content and can realize the making of the bio-reactor of different size of the present invention (volume) with reference to the structure iron of accompanying drawing 1,2,3.
Embodiment one: the laboratory ALR/ff bio-reactor of 2000ml.Be fired into cylindrical shell and spacer (water-bath temperature control with) with withstand voltage glass, constitute inner draft tube, air distribution pipe and annular tube type stream of pulses wall cleaner and reactor head has two point formula inoculation hole with stainless steel.Design variable adopts Ad/Ar=1.158, h/H=0.420.Be used to cultivate medicinal filamentous fungus A.blazei, tank pressure＜0.1kg/cm
2, low ventilation 1: under the 1.0v.v.m. condition, cultivated 6 days, the mycelia dry weight can reach 13.01g/L.So far report adopts this bacterium cultivation results mycelia dry weight of other reactor only up to 3.20g/L both at home and abroad.
Embodiment two: be used as the 1200ml ALR/ff bio-reactor that kind of liquid is cultivated in the laboratory.Making method and requirement are with embodiment one.Design variable then adopts Ad/Ar=1.980, h/H=0.380.Mycelia attitude kind liquid as medicinal filamentous fungus G.sinence is cultivated.Ventilation 1: 2.5v.v.m., tank pressure＜0.1kg/cm
2Not be used in add in the substratum bibliographical information commonly used impel the finely dispersed thickening material of mycelia (as agar, Mierocrystalline cellulose etc.).Cultivated 20 hours, and can obtain to meet the fermentation even neat sarconeme shape kind liquid that requires.Bibliographical information often adopts the interpolation thickener method to make mycelial growth evenly neat, but the adding of thickening material often influences the specification of quality of leavened prod.
Embodiment three: produce with 200 liter ALR/ff bio-reactors.This reactor is made by stainless steel entirely, comprises endless tube shape stream of pulses wall cleaner and two point formula inoculation system (seeing Fig. 1,2,3).Air distribution pipe and vomit pipe are arranged on tank base.Design variable Ad/Ar=1.08, h/H=0.46.Carry out the corresponding valve member of reactor, pipe fitting connection by the fermentation engineering method for normalizing.Carry out with ordinary method that the reactor sky disappears and substratum is real disappears, notice that vapor flow rate that the control air distribution pipe enters is to guarantee the effective stirring of feed liquid in sterilization process.Be used to produce medicinal filamentous fungus Ganoderma fermentation liquid.With 0.5 hours period non-stop run, effect is satisfied with sterile air for the stream of pulses wall cleaner of reactor.Thread kind of liquid with the normal inoculation of two point formula inoculation system 10% inoculum size.In five days cycles, the mycelia dry weight reaches " company standard " requirement, and the mycelium pellet outward appearance that obtains is neat evenly, several aseptic unity piece things (one of leading indicator of this product " company standard ").Compare with relevant bibliographical information, use other reactors also not reach above-mentioned effect (for example, the cenobium caking is the unsetting thing that differs in size, and often need carry out the dispersed processing treatment of secondary).
For a better understanding of the present invention, do following description of drawings.
Fig. 1 ALR/ff bio-reactor primary structure figure
Twi: two point formula inoculation system
Cle: stream of pulses wall cleaner
H: reactor shell height
H: inner draft tube height
D: reactor inside diameter
D: inner draft tube internal diameter
Ad: liquid stream downcomer area in the reactor
Ar: liquid stream ascent stage area in the reactor
The A-A:A-A cross sectional plan view
Air: air admission mouth
Fig. 2 stream of pulses wall cleaner
Cle: stream of pulses wall cleaner
A: by the inlet of controlled valve pulsed inject high pressure sterile air or sterilized water
R: in Φ 3mm pore distribution wholecircle week, every hole is interval 10mm evenly
The partial enlarged drawing of I:I section
Fig. 3 two point formula inoculation system
Twi: two point formula inoculation system
A: two of inoculator are inserted pin and insert diplopore respectively
R: turn on A lid back insertion pin and insert by plug
The partial enlarged drawing of I:I section
Reference: 1) D.J.Pollard, et al.:Bioprocess Engineering. 16:273-281 (1997) 2) Dehua hiu, et al.:Applied Biochemistry and Biotechrology, vol.57/58.pp.593-589.1996.3) K.J.Ganzeveld, et al.:Bioprocess Engineering, 12:239-247 (1995) 4) great waves etc. not: chemical reaction engineering and technology, vol.14.No.1 pp.92-96 (1998) .5) " the 7th national biochemical industry academic conference " collection of thesis, pp.55-58, pp.521-524, pp.525-528 (1996) .6) [U.S.] Leon hapidus, Neal R.Amundson. " Chemical Reactor Theory ", Zhou Peizheng etc. translate, petroleum industry press, 1984.
Claims (7)
1, a kind of bio-reactor that is used for the microbial liquid cultivation is characterized in that this bio-reactor is a kind of airlift reactor with low recirculating zone, includes one or more layers stream of pulses wall cleaner and two point formula inoculation system.
2, the airlift bioreactor with low recirculating zone according to claim 1 is characterized in that the liquid of reactor flows downcomer (Ad) and ascent stage (Ar) area ratio, and promptly the ratio of Ad/Ar parameter designing value is in the scope of 1.00-2.00.
3, the airlift bioreactor with low recirculating zone according to claim 1 is characterized in that the ratio of inner draft tube height h and height for reactor H in the reactor, i.e. parameter designing value≤0.50 of h/H.
4, the airlift bioreactor of low recirculating zone according to claim 1 is characterized in that reactor is provided with the clear wall device of one or more ring-type tubular type stream of pulsess.
5, want the clear wall device of 4 described ring-type tubular type stream of pulsess according to right, a side that it is characterized in that the endless tube relative response wall of the clear wall device of ring-type tubular type pulse has equally distributed Φ 3mm hole, be distributed on the loopful pipe, the hole towards, downward towards reactor wall, best angle is 45 °.
6, method according to claim 1, two point formula inoculation system is characterized in that reactor is provided with two inoculation holes, can link to each other with two end inoculating needles of special-purpose inoculator respectively.
7, reactor head according to claim 6 has two inoculation holes, it is characterized in that inoculating the hole by interchangeable, tightly press, high pressure resistant, high temperature resistant, resilient rubber pad sealed, inoculation liquid can be under equilibrium pressure nature, stably in the inflow reactor, the supplementary unit that matches therewith, be the withstand voltage cylindrical tube of special-purpose inoculator for making by stainless steel, one side has withstand voltage glass liquid level visor, and the cylindrical shell upper and lower end has flexible pipe and inoculating needle to link to each other respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991218949A CN1148438C (en) | 1999-10-22 | 1999-10-22 | Bioreactor for liquid culture of microbes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991218949A CN1148438C (en) | 1999-10-22 | 1999-10-22 | Bioreactor for liquid culture of microbes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1302862A true CN1302862A (en) | 2001-07-11 |
| CN1148438C CN1148438C (en) | 2004-05-05 |
Family
ID=5282175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB991218949A Expired - Fee Related CN1148438C (en) | 1999-10-22 | 1999-10-22 | Bioreactor for liquid culture of microbes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1148438C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100335611C (en) * | 2005-01-26 | 2007-09-05 | 清华大学 | Composite preparing device of circulating and filling strain preparation and biological carrier |
| CN101955881A (en) * | 2009-07-17 | 2011-01-26 | 深圳市每人康生物科技有限公司 | Airlift bioreactor |
| CN101280269B (en) * | 2008-05-28 | 2011-10-05 | 中国人民解放军第三军医大学 | Membrane dialysis reactor for cultivating intestinal flora |
| CN105384240A (en) * | 2015-11-02 | 2016-03-09 | 哈尔滨工业大学宜兴环保研究院 | Artificial active sludge reactor with mycelium pellets as carrier and running method thereof |
| CN108676709A (en) * | 2018-05-11 | 2018-10-19 | 同济大学 | The gas stripping type reactor and process of single cell protein are synthesized for yellow wine lees and rice milk mixed fermentation |
| CN117229881A (en) * | 2023-11-15 | 2023-12-15 | 迪必尔生物工程(上海)有限公司 | Bioreactor for filamentous fungi and application thereof |
-
1999
- 1999-10-22 CN CNB991218949A patent/CN1148438C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100335611C (en) * | 2005-01-26 | 2007-09-05 | 清华大学 | Composite preparing device of circulating and filling strain preparation and biological carrier |
| CN101280269B (en) * | 2008-05-28 | 2011-10-05 | 中国人民解放军第三军医大学 | Membrane dialysis reactor for cultivating intestinal flora |
| CN101955881A (en) * | 2009-07-17 | 2011-01-26 | 深圳市每人康生物科技有限公司 | Airlift bioreactor |
| CN105384240A (en) * | 2015-11-02 | 2016-03-09 | 哈尔滨工业大学宜兴环保研究院 | Artificial active sludge reactor with mycelium pellets as carrier and running method thereof |
| CN108676709A (en) * | 2018-05-11 | 2018-10-19 | 同济大学 | The gas stripping type reactor and process of single cell protein are synthesized for yellow wine lees and rice milk mixed fermentation |
| CN117229881A (en) * | 2023-11-15 | 2023-12-15 | 迪必尔生物工程(上海)有限公司 | Bioreactor for filamentous fungi and application thereof |
| CN117229881B (en) * | 2023-11-15 | 2024-02-06 | 迪必尔生物工程(上海)有限公司 | Bioreactor for filamentous fungi and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1148438C (en) | 2004-05-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1285513C (en) | Fermentation process and fermenter | |
| CN103224965B (en) | Method for producing pyrroloquinoline quinine through microbial fermentation and fermentation medium used in same | |
| EP3190170A1 (en) | Bioreactor with separate co2 supply | |
| CN1148438C (en) | Bioreactor for liquid culture of microbes | |
| Kawagoe et al. | Application of bubble column fermentors to submerged culture of Schizophyllum commune for production of L-malic acid | |
| CN101748054B (en) | Photobioreactor for cultivating microalgae | |
| CN102899239B (en) | Dispersion optical-fiber type photo-bioreactor with inserted spiral band | |
| CN205398612U (en) | Improve photobioreactor of little algae growth rate | |
| CN107177512A (en) | A kind of culture medium for promoting Morciiella Esculeuta Mycelia to grow | |
| CN103224882A (en) | Disposable wave type bioreactor with aerating apparatus | |
| CN209508272U (en) | A kind of High Density Cultivation and collection device of flocculated bacteria | |
| CN100397980C (en) | Airlift biological reactor for culturing non-fixed roots of Danshen | |
| CN102517212B (en) | Guide flow cylinder, cylindrical bioreactor using guide flow cylinder and method for arranging guide flow cylinder | |
| CN208857315U (en) | A kind of plant cell and organ Liquid Culture bag | |
| CN102732416A (en) | Airlift type loop reactor and method for penicillin fermentation by using the same | |
| CN1254531C (en) | Air lift plant cell culture biological reactor with screen mesh guiding drum | |
| CN101182454A (en) | External-internal double-cycling bioreactor | |
| CN104745472A (en) | Plant tissue culture tank | |
| CN1524961A (en) | Microorganism continuous catalysis method for producing acrylamide | |
| CN208414405U (en) | A kind of flat plate photobioreactor | |
| CN201053016Y (en) | Multifunction bioreactor | |
| CN201398323Y (en) | Fermentation device for edible fungi liquid seed production | |
| CN208815010U (en) | A kind of device continuously preparing amino acid strain | |
| CN2078322U (en) | Air-lift biological reactor for high-visco sity fermentation | |
| CN206266628U (en) | Culture device for liquid strain of edible fungus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |