CN1293901C - Chinese medicinal preparation for preventing herpes virus and preparing method thereof - Google Patents
Chinese medicinal preparation for preventing herpes virus and preparing method thereof Download PDFInfo
- Publication number
- CN1293901C CN1293901C CNB021576645A CN02157664A CN1293901C CN 1293901 C CN1293901 C CN 1293901C CN B021576645 A CNB021576645 A CN B021576645A CN 02157664 A CN02157664 A CN 02157664A CN 1293901 C CN1293901 C CN 1293901C
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- ethanol
- extract
- preparation
- less
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention belongs to the technical field of a traditional Chinese medicine preparation and discloses the external application traditional Chinese medicine preparation for resisting herpesvirus. The formulation of the present invention is prepared from traditional Chinese medicine honeysuckle flower used as an active ingredient, isatis root, extractum extracted from wild gingers and pharmaceutical excipient. The present invention adopts a micro-wave extracting technique and a supercritical extraction technique and has the advantages of scientific prescription, advanced process, controllable quality and obvious curative effect.
Description
Technical field:
The invention belongs to the Chinese medicine preparation technical field.Be specifically related to a kind of Chinese medicine preparation and preparation method of anti-herpesvirus.
Background technology:
Herpes is extremely common clinically disease, shows as herpes simplex, genitality herpes and herpes zoster.Herpes simplex virus causes herpes simplex, genitality herpes, mainly is to propagate by skin and mucosa.Abroad, the sickness rate of genitality herpes is only second to gonorrhea and syphilis in sexually transmitted disease (STD), occupies the 3rd of sexually transmitted disease (STD) sickness rate, is being held pride of place in the caused sexually transmitted disease (STD) by virus.Herpes zoster is the skin infection that varicella zoster virus causes.Its oncoming force is fast, pain unbearably and the course of disease long.
U.S. FDA was ratified idoxuridine treatment herpetic keratitis first in 1962.To the end of the year 1999, totally 21 of the anti-herpesvirus medicines of countries in the world approval, nearly 50% anti-herpesvirus medicine systemic administration toxic and side effects is bigger, can only topical treatment eye and skin bleb viral infection.Acyclovir (aciclovir, ACV), valaciclovir (valaciclovir, VCV), famciclovir (famciclovir, FCV) be usually used in treating herpetic disease, " acyclovir " is the most effective medicine of generally acknowledging at present in the medicine of anti-herpesvirus, but it is, relatively poor to recidivist's therapeutic effect improving aspect symptom, the alleviating neuropathic pain a little less than the effect.The treatment of herpes does not also have a kind of efficient ways at present, but because the having a high potential of the market demand, therefore seeking effective antiviral drugs is still the research focus.
Summary of the invention:
Technical problem to be solved by this invention is to design the Chinese medicine of effective anti-herpesvirus.
The invention provides a kind of Chinese medicine for outer use of anti-herpesvirus, said preparation is by Chinese medicine Flos Lonicerae, Radix Isatidis, Herba Asari, Herba Dendranthematis indici, Herba Houttuyniae, the extractum of Herba Artemisiae Annuae extraction and tincture, liniment, ointment, spray, aerosol or the gel etc. that pharmaceutic adjuvant is formed as active component.
The preferred active component percentage by weight of the present invention prescription is Flos Lonicerae 0.1-99.8%, Radix Isatidis 0.1-99.8%, Herba Asari 0.1-99.8%, Herba Dendranthematis indici 0-35%, Herba Artemisiae Annuae 0-30%, Herba Houttuyniae 0-35%, Borneolum Syntheticum 0-10%.
The above-mentioned Chinese medicine of the present invention can be the Chinese crude drug of the different places of production, different cultivars, and by intermediate, Chinese medicine preparation that above-mentioned medical material extracted.
Radix Isatidis is the dry root of cruciferae isatis Isatis indigotica Fort., has heat-clearing and toxic substances removing, antibiotic, antiviral effect, and clearing away heat from blood is monarch drug.
Flos Lonicerae is the dry flower of caprifoliaceae plant Radix Ophiopogonis Lonicera japonica Thunb. Flos Lonicerae Lonicera hypoglauca Miq., Flos Lonicerae Lonicera confusa DC. hair style Radix Ophiopogonis Lonicera dasystyla Rehd. or the flower that band is just opened, has heat-clearing and toxic substances removing, wind-heat dissipating is ministerial drug.
Herba Asari is the dry herb of aristolochiaceae plant Herba Asari Asarum heterotropodesFr.var.mandshuricum (Maxim.) Kitag., Seoul Herba Asari Asarum sieboldiiMiq.var.seoulense Nakai or magnificent Herba Asari Asarum sieboldii Miq., has expelling wind and cold, understand things pain-stopping, antiviral effect.
Herba Houttuyniae is the dry aerial parts of saururaceae plant houttuynia cordata Houtuynia cordata Thunb., has heat-clearing and toxic substances removing, and the effect Herba Artemisiae Annuae of eliminating carbuncle evacuation of pus, inducing diuresis for treating stranguria syndrome is feverfew Herba Artemisiae annuae Artemisia annua L.Dry aerial parts, have heat clearing away knot summer-heat, remove and steam, the effect of preventing the attack (or recurrence) of malaria.
Herba Dendranthematis indici is the dry capitulum of feverfew Herba Dendranthematis indici Chrysanthemum indicum L., has the effect of heat-clearing and toxic substances removing itching relieving.
Borneolum Syntheticum is Dipterocarpaceae plant borneo camphor tree Dryobalanops, the process of resin product of aromatica Gaern f., the bright leaf of feverfew Balsamiferou Blumea Herb Blumea balsamifera DC. is through distillation, the crystalline solid of cooling gained, and the BORNEOLUM SYNTHETICUM of synthetic (Bomeolumsyntneticum), has the refreshment of having one's ideas straightened out, the effect of clearing away heat to alleviate pain.
The effect that the antipruritic analgesic, antiviral of clearing heat, releasing toxin, removing swelling is held back the open sore part is played in the above-mentioned reasonable assembly of medicine of respectively distinguishing the flavor of jointly.
Preparation of the present invention can be used for treating the disease that the human or animal is caused by herpesvirus, particularly herpes simplex keratitis, stomatitis, herpes zoster, genitality herpes.
Herpes simplex and herpes zoster all are viral dermatosiies, use preparation of the present invention (moroxydine preparation), can make the convergence of the herpes open sore part, suppress spreading of virus, make wound healing, incrustation, and can reduce pain, can play obvious effects in general 1-2 days.
The pharmacodynamic study result of preparation of the present invention is as follows:
(1) Radix Isatidis has antibiosis and antiviral functions to various bacteria
1) cell culture experiments:
HR1 (aqueous extraction-alcohol precipitation technology sample), HR2 (aqueous extraction-alcohol precipitation technology sample), HR3 (water is carried the flocculation process sample), HR4 (total water solublity sample), MW2 (microwave induced extraction process sample), each 50 gram of six samples of AT1 (fat-soluble sample), be made into the 30%DMSO suspension with culture fluid, 2 times of dilution variable concentrations add normal and infection cell, measure toxicity, infect and maximal non-toxic concentration was added cell culture in back 2 hours and observe the cytopathic inhibitory action of the first and second type influenza virus.
HR1, HR2, HR3, HR4, AT1 and MW2 maximal non-toxic concentration to first, B-mode weak inhibitory action arranged all, it is invalid to infect prevention administration in preceding 24 hours.
2) oral 1 the acute toxicity of mice:
HR2, HR3,30g/kg is nontoxic;
HR1, HR4 MW2:30g/kg is poisonous, and 25g/kg is nontoxic;
AT1:25g/kg is poisonous, and 15g/kg is nontoxic;
AA2:10g/kg oral 4 days every days 2 times totally 8 times nontoxic.
3) the oral subacute toxicity of mice:
HR1, HR2, HR3, HR4 experiment maximal dose 10g/kg, HR1, HR2 and HR4 oral 4 days every days 2 times totally 8 times nontoxic, HR3:10g/kg is poisonous, 5g/kg is nontoxic;
MW2 maximal dose 5g/kg, oral 4 days every days 2 times totally 8 times nontoxic;
AT1, AA2, maximum 5g/kg oral 4 days every days 2 times totally 8 times poisonous, 2.5g/kg is nontoxic.
(2) Flos Lonicerae
1. external have antibiosis and antiviral functions to various bacteria
Table 2 six samples to the pathological changes caused by virus effect relatively
| Virus | Virazole | 50% valid density (EC 50) ug extract/ml, (IT) | |||||
| 125ug/m 1 | 1 | 2 | 3 | 4 | 5 | 6 | |
| A 3 | ++ | 83.9(6.7) | 167.5(6.6) | 236.6(4.7) | - | 236.6(9.4) | |
| HVJ | ++ | 167.5(3.4) | - | - | - | - | - |
| RSV | + | - | - | - | - | - | - |
| ECHO 11 | + | - | - | - | - | - | - |
| CoxB 4 | + | - | - | - | - | - | - |
| Ad 3 | + | - | - | - | - | - | - |
| Ad 7 | - | - | - | - | - | - | - |
| HSV-I | ++ | - | - | - | - | - | 561.2(4.0) |
| HSV-II | + | - | - | - | - | - | 472.1(4.7) |
"+" is effective "-" for invalid.
No. 1 medicine has inhibitory action to influenza and parainfluenza virus as a result, and 3, No. 4 medicines have in various degree inhibitory action to influenza virus, and No. 6 medicine all has certain inhibitory action to influenza virus, herpesvirus I, II type, and the susceptible toxic action of convection current is the strongest.
2. 1: 4 water decoction is external has slight inhibitory action to some dermatophytess
3. delay the pathological changes effect of Respirovirus pair cell.
4. toxicity is very little, and the median lethal dose(LD 50) of mouse subcutaneous injection is 53g/kg.
Table 3 six samples are to the toxicity test result of Hep-2 cultured cell
| The toxicity test result (the ug extract/ml) | |||||||
| Sample | 1 | 2 | 3 | 4 | 5 | 6 | Virazole |
| CC 50 | 561.8 | 561.8 | 1111.0 | 1111.0 | 1111.0 | 2232.0 | 714.3 |
5. the hot water preserved material has slight preventive effect to rat pylorus ligation gastric ulcer.
(3) Herba Asari
1. local anesthesia effect, water logging agent, pure preserved material (20%-100%) have the infiltration anesthesia effect.Decoct is invalid.
2. antipyretic effect.
3. bacteriostasis
4. toxicity: mouse stomach and intravenous injection, playing a median lethal dose(LD 50) is 123.75mg/10g; 7.78mg/10g Herba Asari leachate toxicity is greater than water decoction.
(4) Herba Houttuyniae
1. antibiotic experiment in vitro shows that micrococcus catarrhalis, hemophilus influenza, streptococcus pneumoniae, staphylococcus aureus are had obvious inhibitory action, proposes a kind of grease from Herba Houttuyniae, many growth of microorganism is had inhibitory action, particularly yeast and mycete.
2. antiviral is cultivated with human embryo kidney (HEK) simple epithelium cell tissue of former generation, and observing Herba Houttuyniae (1: 10) has inhibitory action to the 68-1 of the section strain of first type capital, influenza Asia; HSV1, HSV2 there is inhibitory action.
3. other: analgesia, hemostasis, suppress serous secretion, promote effects such as tissue regeneration.
(5) Herba Artemisiae Annuae water decoction (1: 3) has inhibitory action to some dermatophytes in vitro.
(6) Flos Chrysanthemi Indici
1. antibiosis and antiviral functions: external have inhibitory action to various bacteria staphylococcus aureus, diphtheria corynebacterium and dysentery bacterium; (1: 80) can delay the pathological changes of the metainfective cell of Orphan virus.
2. toxicity is low, and chronic administration does not have the phenomenon of accumulating, and dog is obeyed continuous three weeks of extract 1300mg/kg every day, no serious toxicity reaction, and hepatic and renal function is normal.
Preparation of the present invention is carried out relevant clinical research, and the result is as follows:
1, clinical research
The contrast of peptide butylamine ointment, criterion of therapeutical effect is with reference to " traditional Chinese medical science disease diagnosis criterion of therapeutical effect ", and statistical procedures is with X 2 test.
| Group | The example number | Cure | Effectively | Invalid | Obvious effective rate % | Effective percentage % | |
| Treatment | Herpes simplex (herpes simplex) | 40 | 21 | 14 | 5 | 52.5 | 87.5 |
| Serpentis scurries skin ulcer (herpes zoster) | 42 | 12 | 24 | 6 | 28.6 | 85.7 | |
| Genital herpes | 30 | 18 | 10 | 2 | 60.0 | 93.3 | |
| Contrast | Herpes simplex (herpes simplex) | 30 | 8 | 15 | 7 | 26.7 | 76.7 |
| Serpentis scurries skin ulcer (herpes zoster) | 30 | 3 | 12 | 15 | 10.0 | 50.5 | |
The result shows that treatment group total effective rate is 88.8%, and matched group is 63.3% on year-on-year basis, tool significant difference (P＜0.01)
2, scientific research clinical research
Preparation of the present invention-moroxydine liniment is a pure Chinese medicinal preparation, is used to treat herpes simplex and herpes zoster outward.This product scientific research clinical research purpose is its clinical efficacy of checking and safety, and compares the curative effect of kinds of processes dosage form.
72 routine patients are observed in this checking altogether, inclusion criteria is for having typical local symptom and sign clinically, no General Symptoms or General Symptoms are slight, meet the diagnosis of herpes zoster and facial area, herpes simplex genitalis, the skin lesion time of occurrence is no more than 3 days, anti-herpesvirus medicament is crossed in for oral administration or external in one week, does not use the patient who acts on the immune system medicine in all around.
Treatment group moroxydine liniment, matched group give peptide butylamine ointment, do not use other inside and outside medicines of using in the course of treatment simultaneously.
Curative effect determinate standard: the total mark with symptom and sign after the medication drops to the curative effect basis for estimation, is divided into recovery from illness, produce effects, effective and invalid.Calculate the therapeutic index method: state of an illness total mark * 100% before therapeutic index (state of an illness total mark before the treatment-treatment back state of an illness total mark)/treatment.Recovery from illness is therapeutic index 100%, and produce effects is a therapeutic index 60～90%, effectively is therapeutic index 20～59%, and invalid is therapeutic index＜20%.
Each 36 example of table 1 treatment group and matched group, curative effect is as follows:
| Recovery from illness | Produce effects | Effectively | Invalid | Total effective rate (%) | ||
| The treatment group | Herpes zoster | 3 | 4 | 4 | 1 | 91.7 |
| Herpes simplex | 4 | 5 | 3 | 0 | 100 | |
| Genital herpes | 5 | 5 | 2 | 0 | 100 | |
| Matched group | Herpes zoster | 0 | 2 | 5 | 5 | 58.3 |
| Herpes simplex | 1 | 2 | 4 | 5 | 58.3 | |
| Genital herpes | 1 | 2 | 5 | 4 | 66.7 | |
Treatment group total effective rate is 97.2%, and the matched group total effective rate is 61%, and there were significant differences in contrast.Appearance all has no adverse reaction among two groups of patients.
The above results shows the treatment total effective rate 88.8% of moroxydine liniment group to herpes simplex, herpes zoster, genitality herpes, and matched group Ftibamzone Ointment total effective rate 63.3%, learning check by statistics has significant difference (p＜0.01).Clinical practice shows, this preparation soak affected part, alleviating pain rapidly, wound surface is restrained rapidly, and then the control part is spread, promote open sore part incrustation healing, the moroxydine external preparation has been given full play to the comprehensive function of Chinese medicine---antiviral, pain relieving, antiinflammatory, antipruritic etc., and shorten the course of disease significantly.The characteristics of preparation of the present invention are rapid-action, short treating period, and non-stimulated and other side effect is especially to patient's better effects if of anti-acyclovir.
Another object of the present invention has provided the preparation method of above-mentioned anti-herpesvirus Chinese medicine for outer use.
Active component extractum in the preparation of the present invention is by each component of writing out a prescription being adopted microwave extraction+traditional handicraft extraction, supercritical extraction+traditional handicraft extraction, microwave extraction+supercritical extraction, simple traditional handicraft extract or simple microwave extract method acquisition; The preparation of preparation adopts conventional method to make, and adjuvant is conventional pharmaceutic adjuvant.
Traditional handicraft of the present invention is extracted and is comprised cold-maceration, percolation or water bath reflux method etc.
Microwave-induced extraction of the present invention is meant that medical material after the pulverizing is at power 175W～12KW, solvent strength is 0～95%, extraction time is the extraction of carrying out under 10～60min condition, and wherein said solvent is selected from organic solvents such as water, alcohols, ketone, chloroform, esters, benzene class, ethers.
Supercritical extraction of the present invention is meant the medical material after pulverizing at 33～50 ℃, pressure 80～300kg, and time 30min～3hr, gas flow 50～200kg/hr, ethanol 50～95% is carried secretly or is done without ethanol under the condition of entrainer and extracts.
Preparation of the present invention specifically obtains by following technical proposals:
1, extraction process:
Experiment material and equipment
Medical material: Radix Isatidis Flos Lonicerae Herba Asari Herba Artemisiae Annuae Herba Houttuyniae Herba Dendranthematis indici (all supporting available from Shanghai and hall decoction pieces factory), all the other reagent are analytical pure.
Microwave oven (Whirlpool VIP272) HA-420 supercritical equipment (Nantong), lifting thermostat water bath (Shanghai Shen Sheng Bioisystech Co., Ltd), rotary evaporator (Shanghai Shen Sheng Bioisystech Co., Ltd), electronic balance (going up balance factory of Nereid section), electronic balance FA1004 (going up balance factory of Nereid section), PHSJ-4 type pH meter (going up Nereid Ke Leici), TDL-5-A high speed centrifuge (Anting Scientific Instrument Factory, Shanghai), electric-heated thermostatic water bath (Shanghai balance equipment factory), ZK82J type electric vacunm drying case (Shanghai experimental apparatus head factory), high performance liquid chromatograph Waters510, uniform Design software kit V4.0 (Dongbei Pharmaceutical General Factory grinds civilian institute).
(1) traditional handicraft is extracted:
A, cold-maceration mix medical material, add 0～95% ethanol of 5～10 times of amounts, soak 0～1 year, filter, and merge medicinal liquid, and total amount is 2～6 times of medical material amount; Or
B, percolation mix medical material, soak into 0～1hr, add 0～95% ethanol, exceed liquid level 2～5cm, soak 0～15 day, with the speed percolation of 1～10ml/min, collect percolation then, and total amount is 2～5 times of medical material amount; Or
C, hot reflux are extracted medical material are mixed, 6～12 times of ethanol, consumptions, the extraction time 1～3 time, the time 10～120min that add concentration 0～95%, filter, merge medicinal liquid, be evaporated to 1: 1～1: 2 volume, room temperature placement or cold preservation 1 day～2 days, density 0.97～1.03, pH4.5～5.5 add 50%～95% ethanol to certain volume, and total amount is 2～5 times of medical material amount; Or
(2) microwave-induced extraction
With medical material mix the back directly use, pulverize separately remix or will medical material mix after pulverize again, break into coarse powder or micronizing, under the condition of the concentration 0～95% of power 175W～12KW, solvent, extraction time 10min～60min, soak time 0～0.5hr, extract, medicinal liquid was evaporated to 1: 1, density is 0.97～1.03g/ml, pH4.5～5.5, active ingredient chlorogenic acid content are 1.9～3.1mg/ml, and yield of extract is 5～20%; Or
(3) supercritical extraction is pulverized after medical material is pulverized remix or medical material mixing separately again, break into coarse powder or micronizing, at 33～50 ℃ of temperature, pressure 80～300kg, time 30min～3hr, gas flow 50～200kg/hr, use or do without ethanol under the condition of entrainer and extract, the yield of volatile oil is 0.0544～0.505%;
2, preparation preparation:
Any proportioning by extract 0.01-99.9% and adjuvant 99.9-0.011% is made 100% composition, preparation tincture, liniment, ointment, spray, aerosol, gel, liniment external preparation.
Above-mentioned three kinds of extraction processes are compared as follows active ingredient extract result:
| Chlorogenic acid content | The volatilization oil mass | The extractum amount | |
| Traditional hot refluxes | 2.1-3mg/ml | ≤0.1ml/100g | 15％ |
| Microwave extraction | 1.9～3mg/ml | ＜0.1ml/100g | 10％ |
| Supercritical extraction | 0.35ml/100g | 3％ |
| Extraction time (min) | Solvent load | Impurity | |
| Traditional hot refluxes | 240 | 24 times | 3～13％ |
| Microwave extraction | 60 | 10 times | 2～4％ |
| Supercritical extraction | 180 | 2 times |
By above-mentioned comparative result as seen, the microwave extraction time is short, the efficient height, and solvent load is few, and impurity is few, the purity height, and active constituent content ought with traditional extract phase.
For guaranteeing the abundant extraction of each medical material active ingredient, save solvent simultaneously, improve extract purity, active ingredient extractum of the present invention can adopt microwave extraction and traditional handicraft extract phase in conjunction with (can leave out) or supercritical extraction and combination of traditional handicraft extract phase or microwave extraction with supercritical extraction combines or adopt traditional handicraft separately or adopt the method for microwave extraction separately.
Preparation of the present invention extracts yield and the purity that the Chinese medicine compound effective ingredient can improve product with microwave-assisted extraction technique, reduces extraction time significantly and cuts down the consumption of energy, and makes the product high-quality low-cost, possesses extremely strong competitiveness.The use of supercritical extraction technique can improve stability of drug, can also remove impurity (waxiness, resin), and a step finishes, and reduces operation, raises the efficiency, and saves the energy.
The Chinese medicine preparation prescription science of anti-herpesvirus of the present invention, technology advanced person, quality controllable, determined curative effect.
The specific embodiment:
The preparation of embodiment 1 tincture
Flos Lonicerae 15g, Herba Dendranthematis indici 15g, Radix Isatidis 15g, Borneolum Syntheticum 4g, Herba Asari 9g are mixed, add 5 times of amounts of ethanol of 60%, soaked two months, squeeze and filter is collected medicinal liquid promptly.Chlorogenic acid content is not less than 0.5mg/ml, pH4.5-5.5, ethanol alcoholic degree 45-55%.
The preparation of embodiment 2 ointments
(1) preparation of extract: adopted for two steps, the first step adopts supercritical extraction volatile oil, the second step reuse microwave extraction fixed oil part.Flos Lonicerae 2000g, Radix Isatidis 2000g, Herba Houttuyniae 800g, Herba Asari 720g are mixed, pulverize, in the extraction kettle of packing into, controlled pressure 210kg, 35 ℃ of temperature, extraction time is 1.5 hours, the volatile oil yield is 0.3578%; The fixed oil part, the reuse microwave extraction is got the medicinal residues after the supercritical extraction, adds 50% ethanol 3L, and microwave power 2000W extracted 10 minutes, and filtration is evaporated to (1: 1).Record chlorogenic acid content and be not less than 2.6mg/ml, volatile oil is not less than 0.1ml/100g, yield of extract 9.57%.
(2) formulation preparation
Prescription:
Extract 240g
Cera Flava 25g
Glyceryl monostearate 90g
Vaseline 100g
Liquid Paraffin 60g
Sodium lauryl sulphate 10g
Glycerol 50g
Azone 10g
Distilled water adds to 1000g
Preparation: oil phase Cera Flava, glyceryl monostearate, vaseline, liquid Paraffin are heated to 80 ℃, and water-phase extract, azone, sodium lauryl sulphate, glycerol, water heat a little more than 80 ℃, and water slowly is added to oil phase, and the limit edged stirs, and become breast gradually.
Chlorogenic acid content is not less than 0.5mg/ml, and volatile oil is not less than 0.05ml/100g, minimum loading amount 30g/ box,
The preparation of embodiment 3 sprays
(1) preparation of extract
The employing microwave method extracts, and Flos Lonicerae 150g, Herba Dendranthematis indici 150g, Radix Isatidis 90g, Herba Asari 60g, Herba Houttuyniae 120g are weighed, and mixes, the ethanol 600ml of adding 70% soaked microwave power 595w 30 minutes, extracted 15 minutes, and filtered, be evaporated to (1: 1) 570ml.Record chlorogenic acid content and be not less than 2.6mg/ml, volatile oil is not less than 0.1ml/100g, yield of extract 9.57%.
(2) formulation preparation
Prescription
Chinese medicine extract 400g
Glycerol 50g
Tween 10g
Azone 10g
Ethanol adds to 2500ml
The preparation by the prescription with medicament mixed, fill in spray bottle, every bottle of 30ml.Record chlorogenic acid content and be not less than 1.0mg/ml, volatile oil is not less than 0.04ml%, pH4.5-5.5, ethanol alcoholic degree 45-55%.
The preparation of embodiment 4 gels
Medical material Flos Lonicerae 125g, Radix Isatidis 75g, Herba Asari 45g, Herba Dendranthematis indici 45g, Herba Houttuyniae 75g, Herba Artemisiae Annuae 60g are weighed, mix, use microwave extraction, soak 20min with 90% ethanol 3200ml, microwave power 340w extracts 45min, be evaporated to 320ml, cold preservation, the centrifugal precipitation of removing, 1% activated carbon decolorizing, 60 ℃ of temperature were decoloured 1 hour, add ethylparaben 1g then, Carbopol 94010%, glycerol 50g, Borneolum Syntheticum 20g adds water to 1000g.Chlorogenic acid is not less than 1.0mg/ml, and volatile oil is not less than 0.1ml/100g.
The preparation of embodiment 5 aerosols
(1) Flos Lonicerae 900g, Herba Dendranthematis indici 900g, Radix Isatidis 900g, Herba Asari 540g, Herba Houttuyniae 900g are weighed in the preparation of extract, mix, and add 70% ethanol 40L, soaked 30 minutes, microwave power 4000w extracted 15 minutes, filter, be evaporated to (1: 1) 4L.
(2) formulation preparation
Chinese medicine extract 570g
Glycerol 50g
Tween 10g
Azone 10g
Methacrylate 50g
F12 50g
Propylene glycol 100ml
Ethanol adds to 1000ml
Preparation: Borneolum Syntheticum 4g is dissolved in the ethanol, mixes with other drug in the prescription, and fill is in aerosol bottle.Record chlorogenic acid content and be not less than 1.5mg/ml, volatile oil is not less than 0.1ml/ml, pH4.5-5.5, ethanol alcoholic degree 45-55%.
The preparation of embodiment 6 ointments
(1) the preparation first step of extract adopts supercritical extraction volatile oil, and Flos Lonicerae 2000g, Radix Isatidis 2000g, Herba Houttuyniae 800g, Herba Asari 720g are mixed, pulverize, and in the extraction kettle of packing into, controlled pressure 270kg, 50 ℃ of temperature, extraction time is 3 hours.(the volatile oil yield is 0.3578%); Second step adopted tradition to extract, get medicinal residues 500g after the supercritical extraction,, extract 2 times with 50% ethanol 5000ml hot reflux, each 2 hours, concentrating under reduced pressure adds 2 times of amount ethanol and makes precipitation, and cold preservation is spent the night, centrifugal, get the upper strata, being evaporated to relative density is 1.20mg/ml, stand-by as the traditional handicraft extract.
(2) formulation preparation
Prescription
Supercritical extract 9g
Traditional handicraft extract 300g
Lanoline 50g
Para hydroxybenzene methyl ethyl ester 1g
Vaseline adds to 1000g
Preparation: vaseline, lanoline heating and melting add extract while hot.Record chlorogenic acid content and be not less than 1.5mg/ml, volatile oil is not less than 0.1ml/ml.
The preparation of embodiment 7 liniments
Adopt supercritical extraction (using ethanol to do entrainer) and microwave extraction
The first step adopts supercritical extraction volatile oil, Flos Lonicerae 2000g, Radix Isatidis 2000g, Flos Chrysanthemi Indici 800g, Herba Asari 720g are mixed, pulverize, pack in the extraction kettle controlled pressure 270kg, 50 ℃ of temperature into, add 90% ethanol 3L and do entrainer, extraction time is 3 hours, collects extract 2.8L, the centrifuging and taking supernatant; Medicinal residues microwave extraction behind the supercritical, the ethanol 40L of adding 30%, microwave power 2000W extracted 60 minutes, filtered, and was evaporated to (1: 1), and supercritical extract and microwave extraction thing are merged, and added ethanol to 7000ml.Record chlorogenic acid content and be not less than 1.5mg/ml, volatile oil is not less than 0.1ml/100ml, yield of extract 9.57%.
The preparation of embodiment 8 sprays
Combine with traditional handicraft with microwave extracting
Radix Isatidis 1500g microwave extraction is soaked 20min with 90% ethanol 1200ml, and microwave power 680w extracts 60min, is evaporated to 1500ml; Flos Lonicerae 1500g, Flos Chrysanthemi Indici 1500g, Herba Asari 900g, Herba Artemisiae Annuae 720g mix, and add 70% ethanol 10000ml, soak into 7 days, with the diafiltration of 3ml/min speed, collect percolate, merge the Radix Isatidis extracting solution, add Borneolum Syntheticum 40g, make total amount of liquid reach 15000ml.Fill is in spray bottle, and the 30ml/ bottle records chlorogenic acid content and is not less than 1.5mg/ml, and volatile oil is not less than 0.1ml/100ml, alcoholic degree 45-55%.
Claims (7)
1, a kind of Chinese medicine preparation of anti-herpesvirus is characterized in that the tincture of wherein said Chinese medicine preparation prepares by following method:
Flos Lonicerae 15g, Herba Dendranthematis indici 15g, Radix Isatidis 15g, Borneolum Syntheticum 4g, Herba Asari 9g are mixed, add 5 times of amounts of ethanol of 60%, soaked two months, squeeze and filter is collected medicinal liquid promptly, and chlorogenic acid content is not less than 0.5mg/ml, pH4.5-5.5, ethanol alcoholic degree 45-55%.
2, a kind of Chinese medicine preparation of anti-herpesvirus is characterized in that the spray of wherein said Chinese medicine preparation prepares by following method:
(1) preparation of extract
The employing microwave method extracts, Flos Lonicerae 150g, Herba Dendranthematis indici 150g, Radix Isatidis 90g, Herba Asari 60g, Herba Houttuyniae 120g are weighed, mix, add 70% ethanol 600ml, soaked 30 minutes, microwave power 595w extracted 15 minutes, filtered, be evaporated to 1: 1570ml, record chlorogenic acid content and be not less than 2.6mg/ml, volatile oil is not less than 0.1ml/100g, yield of extract 9.57%;
(2) formulation preparation
The Chinese medicine extract 400g of step (1)
Glycerol 50g
Tween 10g
Azone 10g
Ethanol adds to 2500ml
Preparation: with medicament mixed, fill is in spray bottle, and every bottle of 30ml records chlorogenic acid content and is not less than 1.0mg/ml, and volatile oil is not less than 0.04ml%, pH4.5-5.5, ethanol alcoholic degree 45-55%.
3, a kind of Chinese medicine preparation of anti-herpesvirus is characterized in that the gel of wherein said Chinese medicine preparation prepares by following method:
Medical material Flos Lonicerae 125g, Radix Isatidis 75g, Herba Asari 45g, Herba Dendranthematis indici 45g, Herba Houttuyniae 75g, Herba Artemisiae Annuae 60g are weighed, mix, use microwave extraction, soak 20min, microwave power 340w with 90% pure 3200ml, extract 45min, be evaporated to 320ml, cold preservation, the centrifugal precipitation of removing, 1% activated carbon decolorizing, 60 ℃ of temperature were decoloured 1 hour, added ethylparaben 1g then, Carbopol 94010%, glycerol 50g, Borneolum Syntheticum 20g adds water to 1000g, the not low 1.0mg/ml of chlorogenic acid, volatile oil is not less than 0.1ml/100g.
4, a kind of Chinese medicine preparation of anti-herpesvirus is characterized in that the aerosol of wherein said Chinese medicine preparation prepares by following method:
(1) Flos Lonicerae 900g, Herba Dendranthematis indici 900g, Radix Isatidis 900g, Herba Asari 540g, Herba Houttuyniae 900g are weighed in the preparation of Chinese medicine extract, mix, and add 70% ethanol 40L, soaked 30 minutes, microwave power 4000w extracted 15 minutes, filter, be evaporated to 1: 14L;
(2) formulation preparation
Step (1) Chinese medicine extract 570g
Glycerol 50g
Tween 10g
Azone 10g
Methacrylate 50g
F12 50g
Propylene glycol 100ml
Ethanol adds to 1000ml
Preparation: Borneolum Syntheticum 4g is dissolved in the ethanol, mixes with other drug in the prescription, and fill records chlorogenic acid content and is not less than 1.5mg/ml in aerosol bottle, and volatile oil is not less than 0.1ml/ml, pH4.5-5.5, ethanol alcoholic degree 45-55%.
5, a kind of Chinese medicine preparation of anti-herpesvirus is characterized in that the ointment of wherein said Chinese medicine preparation prepares by following method:
(1) supercritical extraction volatile oil is adopted in the preparation of extract, and Flos Lonicerae 2000g, Radix Isatidis 2000g, Herba Houttuyniae 800g, Herba Asari 720g are mixed, and pulverizes, pack in the extraction kettle controlled pressure 270kg, 50 ℃ of temperature into, extraction time is 3 hours, and the volatile oil yield is 0.3578%; Then, get medicinal residues 500g after the supercritical extraction,, extract 2 times with 50% ethanol 5000ml hot reflux, each 2 hours, concentrating under reduced pressure added 2 times of amount ethanol and makes precipitation, and cold preservation is spent the night, centrifugal, get the upper strata, being evaporated to relative density is 1.20mg/ml, as traditional technology extract is stand-by for the second time;
(2) formulation preparation
Step (1) supercritical extract 9g
Step (1) is traditional technology extract 300g for the second time
Lanoline 50g
Para hydroxybenzene methyl ethyl ester 1g
Vaseline adds to 1000g
Preparation: vaseline, lanoline heating and melting, add extract while hot, record chlorogenic acid content and be not less than 1.5mg/ml, volatile oil is not less than 0.1ml/ml.
6, a kind of Chinese medicine preparation of anti-herpesvirus is characterized in that the liniment of wherein said Chinese medicine preparation prepares by following method:
Adopt supercritical extraction, use ethanol to do entrainer and microwave extract method, the first step adopts supercritical extraction volatile oil, and Flos Lonicerae 2000g, Radix Isatidis 2000g, Flos Chrysanthemi Indici 800g, Herba Asari 720g are mixed, and pulverizes, pack in the extraction kettle, controlled pressure 270kg, temperature adds 90% ethanol 3L for 50 ℃ and does entrainer, and extraction time is 3 hours, collect extract 2.8L, the centrifuging and taking supernatant; Medicinal residues microwave extraction behind the supercritical, the ethanol 40L of adding 30%, microwave power 2000W extracted 60 minutes, filtered, be evaporated to 1: 1, supercritical extract and microwave extraction thing are merged, add ethanol, record chlorogenic acid content and be not less than 1.5mg/ml to 7000ml, volatile oil is not less than 0.1ml/100ml, yield of extract 9.57%.
7, a kind of Chinese medicine preparation of anti-herpesvirus is characterized in that the spray of wherein said Chinese medicine preparation prepares by following method:
Radix Isatidis 1500g microwave extraction is soaked 20min with 90% ethanol 1200ml, and microwave power 680w extracts 60min, is evaporated to 1500ml; Flos Lonicerae 1500g, Flos Chrysanthemi Indici 1500g, Herba Asari 900g, Herba Artemisiae Annuae 720g mix, and add 70% ethanol 10000ml, soak into 7 days, with the diafiltration of 3ml/min speed, collect percolate, merge the Radix Isatidis extracting solution, add Borneolum Syntheticum 40g, make total amount of liquid reach 15000ml, fill is in spray bottle, and the 30ml/ bottle records chlorogenic acid content and is not less than 1.5mg/ml, volatile oil is not less than 0.1ml/100ml, alcoholic degree 45-55%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021576645A CN1293901C (en) | 2002-12-23 | 2002-12-23 | Chinese medicinal preparation for preventing herpes virus and preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021576645A CN1293901C (en) | 2002-12-23 | 2002-12-23 | Chinese medicinal preparation for preventing herpes virus and preparing method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1509741A CN1509741A (en) | 2004-07-07 |
| CN1293901C true CN1293901C (en) | 2007-01-10 |
Family
ID=34236633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021576645A Expired - Fee Related CN1293901C (en) | 2002-12-23 | 2002-12-23 | Chinese medicinal preparation for preventing herpes virus and preparing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1293901C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105362414A (en) * | 2015-12-04 | 2016-03-02 | 钱玲科 | Traditional Chinese medicine composition extract for treating herpes viruses, method for preparing traditional Chinese medicine composition extract and application thereof |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485709B (en) * | 2008-01-16 | 2012-05-23 | 上海中药制药技术有限公司 | Chinese medicinal composition for treating zoster and preparation thereof |
| CN102430071B (en) * | 2011-12-07 | 2013-05-01 | 黄炳炎 | Medicament for treating herpes zoster |
| CN104107258A (en) * | 2014-07-16 | 2014-10-22 | 张金顺 | Medicinal composition for treating viral keratitis |
| CN105250375A (en) * | 2015-11-27 | 2016-01-20 | 史克勇 | Composition for treating herpes zoster |
| CN105311105A (en) * | 2015-11-27 | 2016-02-10 | 史克勇 | Composition for treating herpes zoster |
| CN116850229A (en) * | 2023-07-14 | 2023-10-10 | 上海中药制药技术有限公司 | Preparation method and quality control method of antiviral external preparation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1099634A (en) * | 1993-09-03 | 1995-03-08 | 郭长香 | Medicine for curing herpes zostor and the preparation thereof |
| CN1318395A (en) * | 2000-04-19 | 2001-10-24 | 黄波 | Dermatosis treating medicine and its preparation |
-
2002
- 2002-12-23 CN CNB021576645A patent/CN1293901C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1099634A (en) * | 1993-09-03 | 1995-03-08 | 郭长香 | Medicine for curing herpes zostor and the preparation thereof |
| CN1318395A (en) * | 2000-04-19 | 2001-10-24 | 黄波 | Dermatosis treating medicine and its preparation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105362414A (en) * | 2015-12-04 | 2016-03-02 | 钱玲科 | Traditional Chinese medicine composition extract for treating herpes viruses, method for preparing traditional Chinese medicine composition extract and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1509741A (en) | 2004-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101361765B (en) | Anti-coxsackie and anti-herpesvirus extract of selaginella tamariscina and extraction technique and formulation thereof | |
| CN1768812A (en) | Medicinal composition for removing dampness to relieve itching and its preparation method and uses | |
| CN1293901C (en) | Chinese medicinal preparation for preventing herpes virus and preparing method thereof | |
| CN1682842A (en) | Chinese medicine composition for diminishing-inflammation and detoxication and its preparing method and use | |
| CN1660850A (en) | Method for preparing dehydrogenated cavidine, dehydrogenated apocavidine and combination | |
| CN101396373B (en) | Cinobufacini extract and preparation method thereof | |
| CN1806832A (en) | Pharmaceutical composition, its preparation process and quality control method | |
| CN1565467A (en) | Use of cornel and its extract in preparation alpha-glucosidase inhibitor medicine | |
| CN103977112B (en) | A kind of Chinese medicine treating alcoholic liver | |
| CN1965879A (en) | Chinese medicinal effective parts for treating nasal inflammation and preparation process thereof | |
| CN1840116A (en) | Externally applied medicine for treating skin and mucosa disease caused by human papilloma virus | |
| CN1111045C (en) | Chewing medicine for treating facial paralysis and its preparing process | |
| CN1406585A (en) | Medicinal composition for viral myocarditis | |
| CN1857333A (en) | Aweto bolus and its preparing method | |
| CN108635560A (en) | A kind of Chinese medicine and preparation method thereof for treating lymthoma | |
| CN1201811C (en) | Chinese medicine composition for treating myopia and preparing method thereof | |
| CN1186052C (en) | Medicine for treatment of pelvic inflammation, its preparation and preparing method | |
| CN102846668A (en) | Preparation method for nanometer Periplaneta americana | |
| CN1243560C (en) | Medicine for treating dermatosis and its preparation method | |
| CN1301127C (en) | Technological method of preparing vessels and networks quiet injection preparation | |
| CN1068219C (en) | Slow-released analgesic suppository and its preparation process | |
| CN1189208C (en) | Chinese medicine composition for curing child common cold fever | |
| CN115887519A (en) | Extraction process for effectively extracting microRNA (ribonucleic acid) active ingredients from honeysuckle | |
| CN1552433A (en) | Chinese medicine preparation for treating respiratory tract infection and virus flu, preparing method thereof | |
| CN118477123A (en) | Composition containing broccoli seed water extract for protecting pneumonia and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: SHANGHAI ZHONGHUA PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: SHANGHAI ZHONGHUA PHARMACEUTICAL FACTORY Effective date: 20090904 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20090904 Address after: 139, 1448 lane, West Yan'an Road, Changning District, Shanghai. Zip code: 200052 Co-patentee after: Shanghai TCM Technology Co.,Ltd. Patentee after: Shanghai Zhonghua Pharmaceutical Co.,Ltd. Address before: No. 139, No. 1448 lane, West Yan'an Road, Shanghai, zip code: 200052 Co-patentee before: Shanghai TCM Technology Co.,Ltd. Patentee before: Shanghai Zhonghua Pharmaceutical Factory |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070110 Termination date: 20211223 |