CN1293893C - Gel of Chinese traditional medicine and preparation method - Google Patents
Gel of Chinese traditional medicine and preparation method Download PDFInfo
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- CN1293893C CN1293893C CNB2005100002671A CN200510000267A CN1293893C CN 1293893 C CN1293893 C CN 1293893C CN B2005100002671 A CNB2005100002671 A CN B2005100002671A CN 200510000267 A CN200510000267 A CN 200510000267A CN 1293893 C CN1293893 C CN 1293893C
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Abstract
The present invention belongs to the technical field of pharmacy of traditional Chinese medicine, which specifically discloses gelling agents of traditional Chinese medicine. The crude medicine of gelling agents is composed of catechu, flavescent sophora roots, mint and Chinese toon velamen, and the gelling agents are prepared by catechu extraction, mint volatile oil, extraction of the flavescent sophora roots and the Chinese toon velamen, suitable stroma, transdermal accelerating agents, humectant and solvent. In order to further realize the present invention, crude medicine of the gelling agents is also composed of the catechu, the flavescent sophora roots, the mint, the Chinese toon velamen and Cnidium monnieri. The present invention also discloses a preparation method for the gelling agents; the pharmacological experiment proves that the present invention has better pharmacological action.
Description
Technical field
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of treatment gynaecopathia cervicitis, cervical erosion, colpitic Chinese medicine gel and preparation method thereof.
Technical background
Cervicitis is the most a kind of gynaecopathia of easy trouble of women, and approximately women more than half suffers from cervicitis.Cervicitis has acute and chronic branch, and acute cervicitis mostly occurred in puerperal, or betides acute pelvic inflammatory disease simultaneously, and is often easily out in the cold, and modal clinically be chronic cervicitis.Chronic cervicitis is removed increases patient's misery, influences outside the WomanHealth, also with the certain relation of having of cervical cancer, answers active treatment.Lose because of inflammation when the squamous epithelial cancer of the cervix uteri vagina surface at cervix uteri collar extension place, covered, make the tissue at this position be granular red color area, be called cervical erosion by the columnar epithelium of neck tube.The main pathogenic bacteria that causes cervicitis and cervical erosion is staphylococcus, streptococcus, colibacillus and anaerobe.
Vaginitis is the general name of the multiple vaginal mucosa inflammatory disease that causes of the different causes of disease.At the normal physiological state, the histological anatomy of vagina and biochemistry characteristics are enough to defend the invasion and attack of external microbe.If destroyed, then pathogen can be taken advantage of the occasion and be gone into, and borrows all factors, causes colpitis.
Mainly take physiotherapy and Drug therapy for the Therapeutic Method that above-mentioned disease is present.The conventional means of physiotherapy has electricity to press art, laser therapy or cryotherapy.Ultimate principle all is to cause the inflammation tissue necrosis, come off by physical factor, then in the cervix uteri squamous epithelial tissue that regrows out.Inflammatory tissue comes off though physiotherapy can make, cervical mucosa is newborn repairs, and side effect such as hemorrhage, that water sample oozes out are arranged, and also can shrink because of scar tissue to make narrow, the adhesion of cervical canal, and treat and must carry out in hospital, cumbersome.Oral therapy in the pharmacotherapy, due to illness feelings how repeatedly, the patient often be difficult to adhere to the treatment.External treatment in the pharmacotherapy is because of directly acting on disease sites, with strong points, disturb little to organismic internal environment, it is fast to prove effective, belong to the non-invasive treatment method and clinical rather well received and pay attention to, Chinese medicine external curing cervicitis, cervical erosion and colpitic clinical report are more, and the dosage form of application has lotion, powder, suppository, unguentum, paste etc.From clinical practice, these dosage forms often big, the poor permeability of medicine coated face, mucosa is had stimulation, and shortcoming such as the inconvenience of use is arranged all.
Gel is that a class contains more than two components or two components, by solid-liquid two phase compositions, has the macromolecular network system of semi-solid property.With medicine dissolution or be dispersed in and be gel in the gel, it can the long period and the site of action tight adhesion, and better biocompatibility is arranged, and method for making is simple, uses comfortable.Because formed aquation gel layer has the effect of certain sustained release to medicine behind the gel water absorption and swelling, now has been widely used in slow release, controlled release system.Chinese medicine gel mostly is percutaneous or mucosa delivery, is used for many-sided especially treatment of gynaecopathia such as antiinflammatory, pain relieving, local hemorrhage at present.Chinese medicine gynecological gel kind is also fewer, and patent (ZL01126561) discloses a kind of Chinese medicine gel, and this medicine is made up of Catechu extract and Radix Sophorae Flavescentis total alkaloids, is used for the treatment of cervicitis and cervical erosion; Patent (ZL02135793) discloses a kind of matrine vaginal gel that is used for the treatment of cervicitis.
In patent retrieval, do not find any report of any relevant gel of the present invention.
Summary of the invention
The objective of the invention is to disclose a kind of good effect, easy to use, treatment cervicitis, cervical erosion, colpitic Chinese medicine gel that side effect is little.
Another object of the present invention is the preparation method that discloses above-mentioned Chinese medicine gel.
The present invention is achieved through the following technical solutions:
One, preparation method
1. process recipes:
(1) the raw material medicines in portions by weight proportioning is: catechu 2-4 part, Radix Sophorae Flavescentis 8-16 part, Herba Menthae 4-8 part, Cortex Ailanthi 7-13 part;
(2) get Herba Menthae, be cut into segment, add 6-8 times of water gaging warm macerating after 4-5 hour, extract volatile oil, get Herba Menthae Haplocalycis volatile oil with steam distillation;
(3) get catechu 40%-80% alcohol reflux three times, each 1 hour, merge ethanol extract, decompression recycling ethanol also concentrates, and vacuum drying gets Catechu extract;
(4) get Radix Sophorae Flavescentis, Cortex Ailanthi decocts with water secondary, 2 hours for the first time, 1 hour for the second time, merges decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract.
2. formulation preparation:
Chinese medicine gel of the present invention comprises following parts by weight of component:
Herba Menthae Haplocalycis volatile oil 0.3-0.7 part, Catechu extract 12-24 part,, Radix Sophorae Flavescentis, Cortex Ailanthi extract 16-30 part, substrate 0.5-4 part, transdermal enhancer 0.5-1 part, wetting agent 5-20 part, solvent 34.5-51.5 part.
The present invention is prepared from by the following method:
Get substrate, the transdermal enhancer of recipe quantity, the adding distil water swelling, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, get Catechu extract, Herba Menthae Haplocalycis volatile oil dissolves with glycerite, under agitation dropwise adds in the above-mentioned substrate, stir and promptly get Chinese medicine gel of the present invention;
Catechu in the preparation of the present invention has removing dampness and holds back the skin ulcer effect, and Radix Sophorae Flavescentis has that the heat clearing away bath is wet, parasite killing, diuresis effect, and Herba Menthae has dispelling wind-heat, refresh oneself, rash effect, and Cortex Ailanthi has heat clearing and damp drying, astringent therapy leukorrhagia stopping, antidiarrheal, hemostasia effect.More than each medicine share, the wet power of heat clearing away bath is stronger, it is puckery to receive energy, has therapeutical effect preferably for cervicitis, cervical erosion and vaginitis.
The administration that gel continues in the part of patient body, drug level in the maintenance local blood is in effective therapeutic domain, treatment for the low position of oral back blood drug level is very much suitable, medicine in the gel absorbs through skin diffusion, avoided gastrointestinal and liver to stimulate to the medicine first pass effect with to gastrointestinal, gel can also reduce the frequency of administration greatly, reduce to absorb metabolic individual variation, thereby can the short period, high efficiency treatment disease.Thereby the invention medicine is made gel more adapt to gynaecopathia cervicitis, cervical erosion, colpitic disease feature.
Because contained medicine has certain selectivity for transdermal enhancer in the gel, research worker of the present invention confirms through a large amount of tests, has selected to be suitable for transdermal enhancer of the present invention and consumption thereof.Oleum menthae in the preparation also plays the effect of certain transdermal enhancer simultaneously, can promote the Transdermal absorption of medicine.
The used substrate of gel of the present invention can be in carbomer, polyacrylamide, cross linked sodium polyacrylate, methylcellulose, sodium carboxymethyl cellulose, hydroxypropyl emthylcellulose, tragcanth, xanthan gum, the alginic acid a kind of, its weight percentage is 0.5%-4%.
The used wetting agent of the present invention is a glycerol; The used solvent of the present invention is a distilled water.
For further realizing being achieved through the following technical solutions purpose of the present invention: described Chinese medicine gel of the present invention, the raw material medicines in portions by weight proportioning is:
Catechu 2-4 part, Radix Sophorae Flavescentis 8-16 part, Herba Menthae 4-8 part, Cortex Ailanthi 7-13 part, Fructus Cnidii 4-8 part;
Method for making: get Herba Menthae, add 6-8 times of water gaging warm macerating after 4-5 hour, extract volatile oil, get Herba Menthae Haplocalycis volatile oil with steam distillation; Get catechu, Fructus Cnidii 40%-80% alcohol reflux three times, each 2 hours, merge ethanol extract, decompression recycling ethanol also concentrates, and vacuum drying gets catechu, Fructus cnidii extract; Get Radix Sophorae Flavescentis, Cortex Ailanthi decocts with water secondary, 2 hours for the first time, 1 hour for the second time, merges decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract.
Get substrate, the transdermal enhancer of recipe quantity as stated above, the adding distil water swelling, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, get catechu, Fructus cnidii extract, Herba Menthae Haplocalycis volatile oil and dissolve, under agitation dropwise add in the above-mentioned substrate, stir and promptly get Chinese medicine gel of the present invention with glycerite;
Pharmacological evaluation proof Chinese medicine gel of the present invention has pharmacological action preferably.
Two, the assay of preparation
Reagent: gel 1 (according to the gel of patent ZL01126561 method preparation);
Gel 2 (Chinese medicine gel of the present invention, Tianzhijiao Medication Development Co., Ltd., Guangdong's preparation).
1. the assay of matrine and sophoridine
1.1 chromatographic condition: APS2Hypersil (250mm * 4.6mm, 5 μ m); Mobile phase: acetonitrile-water (10: 1) (it is 2.1 that phosphoric acid is transferred pH); Detect wavelength: 207nm; Flow velocity: 1ml/min.
1.2 assay method: precision takes by weighing matrine reference substance and sophoridine reference substance, and with 50% methanol wiring solution-forming, making matrine content is 1.2mg/ml, and sophoridine content is 1.0mg/ml; Get this product 0.05g, put in the 100ml volumetric flask, with acetonitrile-water (10: 1) dissolving, standardize solution shakes up, and filters, i.e. sample solution; Get reference substance solution and sample solution 5 μ l respectively, inject high performance liquid chromatograph, measure and calculate, the results are shown in Table 1.
2. the assay of catechin and epicatechin
2.1 chromatographic condition: diamonsil C18 post (150mm * 3mm, 5 μ m), mobile phase: acetonitrile-water (10: 90); Detect wavelength: 280nm; Flow velocity: 1ml/min.
2.1 assay method: precision takes by weighing catechin reference substance and epicatechin reference substance, with 50% methanol wiring solution-forming, making catechin content is 0.4mg/ml, epicatechin content is 0.03mg/ml: get this product 2g, put in the 25ml volumetric flask, with 50% methanol supersound process 15 minutes, and add 50% methanol constant volume to scale, shake up, filter, i.e. sample solution; Get reference substance solution and sample solution 10 μ l respectively, inject high performance liquid chromatograph, measure and calculate, the results are shown in Table 1.
The assay of table 1 preparation (mg/g)
| Group | Matrine | Sophoridine | Catechin | Epicatechin |
| Gel 1 gel 2 | 22.2 9.87 | 25.4 11.3 | 27.6 27.5 | 1.74 1.72 |
Active constituent content with above-mentioned control indexes in the Chinese medicine gel of the present invention is lower than gel 1, and the aftermentioned pharmacological evaluation shows that the pharmacological action of Chinese medicine gel of the present invention is better than gel 1, and effect is more comprehensive, shows prescription of the present invention science more rationally.
Three, the selection of transdermal enhancer
Medicine is brought into play curative effect by the absorption of skin or mucosa, must possess characteristics such as molecular weight is little, polarity is low, but most drug does not possess these characteristics, and therefore normal adding penetration enhancer is to improve the absorption of medicine.Skin comprises organic solvent, surfactant, anvil alkene class, Laurel nitrogen cover ketone and homologue thereof etc. with penetration enhancer, and its mechanism of action is and its dissolving skin lipid, or makes the skin protein degeneration, or to medicine produce solubilising or the hydrotropy effect relevant.Azone is a kind of nonpolar penetration enhancer, and its sphere of action is wide, no color or smell, and zest is low, and hydrophilic and oleophilic type medicine is all had short saturating effect.Document (Cui is burned etc. and Mentholum promotes chloromycetin transdermal penetration effect research. the Chinese Hospitals pharmaceutical journal, 1996,31 (8): 457.) research shows that also menthol and azone have the collaborative drug transdermal effect that strengthens, because preparation of the present invention contains the menthol effective ingredient, therefore this test is an index with the effective ingredient kurarinone of Radix Sophorae Flavescentis and the skin permeation rate of sophoridine, select azone as transdermal enhancer, and its consumption has been carried out further investigation.
Experimental apparatus and reagent: Waters high performance liquid chromatograph; Improvement Franz diffusion cell (the organic institute in Chinese Academy of Sciences Shanghai); 81-2 type magnetic stirring apparatus (Shanghai Si Le instrument plant); Azone and other reagent are analytical pure.
Experimental technique: select 180～220g healthy rat.Belly wool is cut off, put to death, take off skin of abdomen, remove subcutaneous fat, normal saline is cleaned, and cold preservation is standby, with the skin keratin aspect of above-mentioned processing, pastes repeatedly 20 times with adhesive tape, and horny layer is peelled off, and is smooth surface to skin, and is translucent.Skin is fixed in giving coyote hole and accepting between the pond of improvement Franz diffusion cell, and corium one side contacts with acceptable solution, the emptying bubble.Get the Chinese medicine gel sample of the present invention that certain area contains same matrix, wetting agent, different transdermal enhancers and be attached to, make it to contact closely with skin on the coyote hole skin.Contact liquid is the phosphate buffer of about 10mlpH7.4, and magnetic agitation speed is about 100r/min, and bath temperature is (37 ± 0.5) ℃.In 24 hours, incline and get whole acceptable solutions, replenish the equal-volume acceptable solution then immediately every certain hour.The acceptable solution that takes out places dry evaporating dish, adds 50% methanol constant volume behind the water bath method in the 1ml measuring bottle.Content assaying method according to above-mentioned preparation is measured matrine and sophoridine, calculates skin permeation rate.The results are shown in Table 2.
Table 2 transdermal enhancer is selected test
| Numbering | Transdermal enhancer content (%) | Skin permeation rate (%) |
| 1 2 3 4 5 | 0.25 0.5 1 2 3 | 23.12 35.43 34.08 28.59 28.46 |
By above test data as can be seen, the weight percentage of azone is 0.5%-1%, and the transdermal facilitation effect is better, illustrates that Chinese medicine gel of the present invention has selectivity to transdermal enhancer, the azone weight percentage all has transdermal facilitation preferably in the scope of 0.5%-1%.
Four, vaginal mucosa irritation test
In order to investigate the safety of Chinese medicine gel of the present invention, we have carried out the vaginal mucosa irritation test.
Test material: healthy rabbits (2.0-2.5kg), female; Chinese medicine gel of the present invention is provided by Tianzhijiao Medication Development Co., Ltd., Guangdong.
The test of a coating vaginal mucosa irritation: 10 of Female rabbits, vagina is coated with Chinese medicine gel of the present invention.Clean medicine with warm water in 24 hours behind the coating, after removal is subjected to the reagent thing, observed its skin irritation reaction in 1,24,72 hours.
Repeatedly coating vaginal mucosa irritation experiment: 10 of Female rabbits, vagina are coated with Chinese medicine gel of the present invention, every day 1 time, continuous 7 days.Clean medicine with warm water in 24 hours behind last 1 coating, after removal is tried thing, observed its skin irritation reaction in 1,24,72 hours.
The result: after the rabbit vagina one or many used Chinese medicine gel of the present invention, vaginal mucosa was not seen reaction such as the skin irritation that erythema, edema occur, shows that Chinese medicine gel zest of the present invention is less.
Five, pharmacological testing
Reagent and animal: Chinese medicine gel of the present invention (Tianzhijiao Medication Development Co., Ltd., Guangdong's laboratory provides); Gel 1 (by the gel of patent ZL01126561 method preparation); KANGGONGYAN PIAN (Jiangxi Jing Tong pharmaceutical Co. Ltd); Staphylococcus albus, escherichia coli, bacillus pyocyaneus, staphylococcus epidermidis, streptococcus anaerobius, Bacillus proteus, Candida albicans provide by Nat'l Pharmaceutical ﹠ Biological Products Control Institute; Rat, mice are provided by Guangdong Province's Experimental Animal Center.
1. antibacterial experiment
The filter paper method is measured bacteriostasis: the filter paper of cut-off footpath 6mm, put into and respectively organize drug solution (being mixed with same medicine concentration) immersion 16h, 120 ℃ of following moist heat sterilizations are standby. respectively get 0.5ml and corresponding solid medium with above-mentioned for examination bacterium liquid, in superclean bench, make and contain the bacterium flat board, getting the filter paper that contains diffusion juice is attached to and contains on the bacterium flat board, every ware pastes 5, every bacterium is cooked 3 repetitions, antibacterial is put and cultivates the inhibition zone size that 48h. measures filter paper behind 24h, the 48h in 37 ℃ of biochemical incubators, and relatively fungistatic effect sees Table 3.
Table 3 pair inhibition effect for the examination strain
| For the examination strain | KANGGONGYAN PIAN | Gel 1 | Gel of the present invention | |||
| Bacteriostatic diameter (mm) | Bacteriostatic diameter (mm) | Bacteriostatic diameter (mm) | ||||
| 24h | 48h | 24h | 48h | 24h | 48h | |
| Staphylococcus albus Escherichia coli Pseudomonas aeruginosa MRSE streptococcus anaerobius proteus Candida albicans | 8.5 10.0 5.0 8.5 7.5 7.0 8.5 | 8.0 10.5 4.5 8.5 7.0 7.0 8.5 | 8.5 9.5 5.0 8.5 7.5 7.5 8.5 | 8.0 10.0 5.0 8.5 7.5 7.0 8.0 | 9.5 11.0 6.5 9.5 8.0 8.5 9.0 | 9.0 11.5 6.5 9.0 8.5 8.0 9.0 |
Above-mentioned bacteriostatic experiment shows that Chinese medicine gel of the present invention relatively has better bacteriostasis with the gel 1, the KANGGONGYAN PIAN that prepare by patented method.
2. antiinflammatory experiment
2.1 xylol causes the inhibitory action of mice ear
40 of female mices (body weight 18-22g) divide 4 groups at random, blank group, KANGGONGYAN PIAN group, 1 group of gel, Chinese medicine gel group of the present invention.The blank group is irritated stomach and is given normal saline, KANGGONGYAN PIAN group gastric infusion, and gel 1, Chinese medicine gel group of the present invention are applied to vault place (dosage press clinical dosage respectively and converted) behind the mouse vagina.Each organizes administration every day 1 time, and successive administration 5 days behind the 5th day medicine 2 hours, is applied to mice left side ear with dimethylbenzene 0.02ml, mice is put to death in dislocation behind the 1h, cuts two ears, and punching is weighed, with left ear and auris dextra sheet weight difference is the swelling degree, calculates swelling rate and suppression ratio, the results are shown in Table 4.
Table 4 xylol causes the inhibitory action (X ± SD) of mice ear
| Group | Mus number (only) | Ear swelling rate (%) | Suppression ratio (%) |
| Matched group KANGGONGYAN PIAN group gel 1 gel group of the present invention | 10 10 10 10 | 126.12±43.42 84.81±36.30 * 85.90±34.16 * 61.24±30.35 ** | - 33.3 31.9 51.4 |
Annotate: compare with the blank group:
*P＜0.05:
*P＜0.01.
Above pharmacological testing shows, Chinese medicine gel of the present invention, gel 1, the equal xylol of KANGGONGYAN PIAN causes mice ear that inhibitory action is arranged; Chinese medicine gel of the present invention is compared with gel 1, KANGGONGYAN PIAN, and xylol causes the inhibition better effects if of mice ear.
3. analgesic test
3.1 the mice hot plate is caused the influence of pain effect
40 of female mices (body weight 18-22g) divide 4 groups at random, grouping and medication are with 2.1, successive administration 5 days, 1h is individually fixed in each Mus in 55 ± 0.5 ℃ the hot-plate instrument after the last administration, to lick the indicator reaction of metapedes as " pain ", mice the results are shown in Table 5 to the hot plate response time bitterly after observing administration.
3.2 the influence of the writhing response that Dichlorodiphenyl Acetate causes
Get 40 of female mices (body weight 18-22g), grouping and medication be with 2.1, successive administration 5 days, and 1h after the last administration, lumbar injection 0.6% acetum 0.2ml/20g body weight is observed the mouse writhing number of times in the 10min, the results are shown in Table 5.
Table 5 analgesic test result (X ± SD)
| Group | Mus number (only) | The hot plate pain response time (s) | Turn round the body number of times |
| Blank group KANGGONGYAN PIAN group gel 1 gel group of the present invention | 10 10 10 10 | 11.4±4.2 16.5±4.5 * 16.1±3.9 * 19.3±4.0 ** | 29.0±4.8 18.7±3.5 * 18.5±3.3 * 13.5±2.8 ** |
Annotate: compare with the blank group:
*P＜0.05;
*P＜0.01
Result of the test shows that Chinese medicine gel of the present invention has analgesic activity preferably, compares with gel 1, KANGGONGYAN PIAN, and the analgesic activity of preparation of the present invention is more remarkable.
4. the anti-test of itching
4.1 histamine phosphate is brought out the influence that causes the reaction of itching
40 of rats (body weight 180-220g) are divided into 3 groups at random, and 10 every group, male and female half and half, i.e. blank group, 1 group of gel, gel group of the present invention.Two kinds of gels are added warm water be made into isocyatic solution.Tested preceding 1 day, and with the left back sufficient back wool Ex-all of rat, smeared respectively, the blank group is smeared the distilled water of equivalent every day, tests the same day, abrades unhairing place, left instep, area 1.5cm with coarse sandpaper
2Each organizes the repaste medicine 1 time, behind the 10min, drip 0.01% histamine phosphate liquid 0.05ml, after this comply with 0.02% every 3min every Mus of site of injury, 0.03%, 0.04%...... progressive concentration, the each 0.05ml of every Mus later licks metapedes until rat occurring, with the histamine phosphate total amount that occurs at last being given when rat later licks metapedes is itch-threshold, writes down and carries out statistical procedures.The results are shown in Table 6.
Table 6 pair histamine phosphate brings out the influence (X ± SD) that causing the reaction of itching
| Group | Mus number (only) | Itch-threshold (μ g) |
| 1 group of gel group of the present invention of blank group gel | 10 10 10 | 55.0±21.2 225.5±49.3 ** 320.2±71.4 **Δ |
Annotate: compare with matched group:
*P＜0.01: compare with the BAOFUKANG SHUAN group:
ΔP＜0.05.
Found out that by above result of the test gel of the present invention has tangible itching-relieving action, pharmacological action is better than 1 group of gel.
Six, preparation embodiment
Following examples are intended to further specify rather than limit the present invention.
Embodiment 1
1. extraction process
Take by weighing catechu 30g, Radix Sophorae Flavescentis 120g, Herba Menthae 60g, Cortex Ailanthi 100g; Wherein Herba Menthae is cut into segment, extracts volatile oil with steam distillation, gets Herba Menthae Haplocalycis volatile oil 0.5g; Catechu is with 40% alcohol reflux three times, each 1 hour, merge ethanol extract, decompression recycling ethanol also concentrates, vacuum drying, Catechu extract 18.5g.Radix Sophorae Flavescentis, Cortex Ailanthi decoct with water secondary, 2 hours for the first time, 1 hour for the second time, merge decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract 22g.
2. formulation preparation
Preparation prescription: Herba Menthae Haplocalycis volatile oil 0.5g, Catechu extract 18.5g, Radix Sophorae Flavescentis, Cortex Ailanthi extract 22g, carbomer 0.5g, azone 1g, glycerol 10g, distilled water 47.5g.
Get carbomer, azone, add water-soluble expanding, after getting Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the swollen substrate, it is standby to stir, and glycerol adds water and is made into 40% solution, get Catechu extract, Herba Menthae Haplocalycis volatile oil dissolves with 40% glycerol, under agitation dropwise add in the above-mentioned substrate, add water to 100g, stir and promptly get Chinese medicine gel of the present invention.
Embodiment 2
1. extraction process
Take by weighing catechu 30g, Radix Sophorae Flavescentis 120g, Herba Menthae 60g, Cortex Ailanthi 100g; Wherein Herba Menthae is cut into segment, extracts volatile oil with steam distillation, gets Herba Menthae Haplocalycis volatile oil 0.48g; Catechu is with 50% alcohol reflux three times, each 1 hour, merge ethanol extract, decompression recycling ethanol also concentrates, vacuum drying, Catechu extract 19g.Radix Sophorae Flavescentis, Cortex Ailanthi decoct with water secondary, 2 hours for the first time, 1 hour for the second time, merge decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract 22.5g.
2. formulation preparation
Preparation prescription: Herba Menthae Haplocalycis volatile oil 0.48g, Catechu extract 19g, Radix Sophorae Flavescentis, Cortex Ailanthi extract 22.5g, carbomer 1g, azone 0.5g, glycerol 5g, distilled water 51.5g.
Get carbomer, the azone of recipe quantity, add water-soluble expanding, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, and glycerol adds water and is made into 20% solution, get Catechu extract, Herba Menthae Haplocalycis volatile oil dissolves with 20% glycerol, under agitation dropwise add in the above-mentioned substrate, add water to 100g, stir and promptly get Chinese medicine gel of the present invention.
Embodiment 3
1. extraction process
Take by weighing catechu 30g, Radix Sophorae Flavescentis 120g, Herba Menthae 60g, Cortex Ailanthi 100g; Wherein Herba Menthae is cut into segment, extracts volatile oil with steam distillation, gets Herba Menthae Haplocalycis volatile oil 0.50g; Catechu is with 60% alcohol reflux three times, each 1 hour, merge ethanol extract, decompression recycling ethanol also concentrates, vacuum drying, Catechu extract 20g.Radix Sophorae Flavescentis, Cortex Ailanthi decoct with water secondary, 2 hours for the first time, 1 hour for the second time, merge decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract 21g.
2. formulation preparation
Preparation prescription: Herba Menthae Haplocalycis volatile oil 0.5g, Catechu extract 20g, Radix Sophorae Flavescentis, Cortex Ailanthi extract 21g, alginic acid 2g, azone 1g, glycerol 15g, distilled water 34.5g.
Get carbomer, the azone of recipe quantity, add water-soluble expanding, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, and glycerol adds water and is made into 50% solution, get Catechu extract, Herba Menthae Haplocalycis volatile oil dissolves with 50% glycerol, under agitation dropwise add in the above-mentioned substrate, add water to 100g, stir and promptly get Chinese medicine gel of the present invention.
Embodiment 4
1. extraction process
Take by weighing catechu 20g, Radix Sophorae Flavescentis 80g, Herba Menthae 40g, Cortex Ailanthi 70g; Wherein Herba Menthae is cut into segment, extracts volatile oil with steam distillation, gets Herba Menthae Haplocalycis volatile oil 0.3g; Catechu is with 70% alcohol reflux three times, each 1 hour, merge ethanol extract, decompression recycling ethanol also concentrates, vacuum drying, Catechu extract 12g.Radix Sophorae Flavescentis, Cortex Ailanthi decoct with water secondary, 2 hours for the first time, 1 hour for the second time, merge decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract 16g.
2. formulation preparation
Preparation prescription: Herba Menthae Haplocalycis volatile oil 0.3g, Catechu extract 12g, Radix Sophorae Flavescentis, Cortex Ailanthi extract 16g, xanthan gum 3g, azone 0.5g, glycerol 20g, distilled water 48.2g.
Get carbomer, the azone of recipe quantity, add water-soluble expanding, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, and gets glycerol and adds water and be made into 50% solution, get Catechu extract, Herba Menthae Haplocalycis volatile oil dissolves with 50% glycerol, under agitation dropwise add in the above-mentioned substrate, add water to 100g, stir and promptly get Chinese medicine gel of the present invention.
Embodiment 5
1. extraction process
Take by weighing catechu 40g, Radix Sophorae Flavescentis 160g, Herba Menthae 80g, Cortex Ailanthi 130g; Wherein Herba Menthae is cut into segment, extracts volatile oil with steam distillation, gets Herba Menthae Haplocalycis volatile oil 0.7g; Catechu is with 80% alcohol reflux three times, each 1 hour, merge ethanol extract, decompression recycling ethanol also concentrates, vacuum drying, Catechu extract 24g.Radix Sophorae Flavescentis, Cortex Ailanthi decoct with water secondary, 2 hours for the first time, 1 hour for the second time, merge decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract 30g.
2. formulation preparation
Preparation prescription: Herba Menthae Haplocalycis volatile oil 0.7g, Catechu extract 24g, Radix Sophorae Flavescentis, Cortex Ailanthi extract 30g, sodium carboxymethyl cellulose 4g, azone 1g, glycerol 10g, distilled water 35.3g.
Get carbomer, the azone of recipe quantity, add water-soluble expanding, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, and gets glycerol and is made into 30% solution, get Catechu extract, Herba Menthae Haplocalycis volatile oil dissolves with 30% glycerol, under agitation dropwise add in the above-mentioned substrate, add water to 100g, stir and promptly get Chinese medicine gel of the present invention.
Embodiment 6
1. extraction process
Take by weighing catechu 30g, Radix Sophorae Flavescentis 120g, Herba Menthae 60g, Cortex Ailanthi 100g, Fructus Cnidii 60g; Wherein Herba Menthae is cut into segment, extracts volatile oil with steam distillation, gets Herba Menthae Haplocalycis volatile oil 0.5g; Catechu, Fructus Cnidii be with 40% alcohol reflux three times, each 2 hours, merge ethanol extract, decompression recycling ethanol also concentrates, vacuum drying, catechu, Fructus cnidii extract 30g.Radix Sophorae Flavescentis, Cortex Ailanthi decoct with water secondary, 2 hours for the first time, 1 hour for the second time, merge decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract 22g.
2. formulation preparation
Preparation prescription: Herba Menthae Haplocalycis volatile oil 0.5g, catechu, Fructus cnidii extract 32g, Radix Sophorae Flavescentis, Cortex Ailanthi extract 22.5g, hydroxypropyl emthylcellulose 2g, azone 0.5g, glycerol 10g, distilled water 32.5g.
Get recipe quantity polyacrylamide, azone, add water-soluble expanding, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, and gets glycerol and is made into 30% solution, getting catechu, Fructus cnidii extract, Herba Menthae Haplocalycis volatile oil dissolves with 30% glycerol, under agitation dropwise add in the above-mentioned substrate, add water to 100g, stir and promptly get Chinese medicine gel of the present invention.
Embodiment 7
1. extraction process
Take by weighing catechu 20g, Radix Sophorae Flavescentis 80g, Herba Menthae 40g, Cortex Ailanthi 70g, Fructus Cnidii 40g; Wherein Herba Menthae is cut into segment, extracts volatile oil with steam distillation, gets Herba Menthae Haplocalycis volatile oil 0.35g; Catechu, Fructus Cnidii be with 60% alcohol reflux three times, each 2 hours, merge ethanol extract, decompression recycling ethanol also concentrates, vacuum drying, catechu, Fructus cnidii extract 18g.Radix Sophorae Flavescentis, Cortex Ailanthi decoct with water secondary, 2 hours for the first time, 1 hour for the second time, merge decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract 17g.
2. formulation preparation
Preparation prescription: Herba Menthae Haplocalycis volatile oil 0.35g, catechu, Fructus cnidii extract 18g, Radix Sophorae Flavescentis, Cortex Ailanthi extract 17g, cross linked sodium polyacrylate 1g, azone 1g, glycerol 20g, distilled water 42.65g.
Get recipe quantity polyacrylamide, azone, add water-soluble expanding, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, and gets glycerol and is made into 50% solution, getting catechu, Fructus cnidii extract, Herba Menthae Haplocalycis volatile oil dissolves with 50% glycerol, under agitation dropwise add in the above-mentioned substrate, add water to 100g, stir and promptly get Chinese medicine gel of the present invention.
Embodiment 8
1. extraction process
Take by weighing catechu 40g, Radix Sophorae Flavescentis 160g, Herba Menthae 80g, Cortex Ailanthi 130g, Fructus Cnidii 80g; Wherein Herba Menthae is cut into segment, extracts volatile oil with steam distillation, gets Herba Menthae Haplocalycis volatile oil 0.65g; Catechu, Fructus Cnidii be with 80% alcohol reflux three times, each 2 hours, merge ethanol extract, decompression recycling ethanol also concentrates, vacuum drying, catechu, Fructus cnidii extract 35g.Radix Sophorae Flavescentis, Cortex Ailanthi decoct with water secondary, 2 hours for the first time, 1 hour for the second time, merge decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract 32g.
2. formulation preparation
Preparation prescription: Herba Menthae Haplocalycis volatile oil 0.65g, catechu, Fructus cnidii extract 35g, Radix Sophorae Flavescentis, Cortex Ailanthi extract 32g, polyacrylamide 4g, azone 0.5g, glycerol 5g, distilled water 27.85g.
Get recipe quantity polyacrylamide, azone, add water-soluble expanding, get Radix Sophorae Flavescentis, Cortex Ailanthi extract adding distil water heating for dissolving, add in the substrate, it is standby to stir, and gets glycerol and is made into 20% solution, getting catechu, Fructus cnidii extract, Herba Menthae Haplocalycis volatile oil dissolves with 20% glycerol, under agitation dropwise add in the above-mentioned substrate, add water to 100g, stir and promptly get Chinese medicine gel of the present invention.
Claims (6)
1, a kind of treatment cervicitis, cervical erosion, colpitic Chinese medicine gel is characterized in that the raw material medicines in portions by weight proportioning is:
Catechu 2-4 part, Radix Sophorae Flavescentis 8-18 part, Herba Menthae 4-8 part, Cortex Ailanthi 7-13 part.
2, according to the Chinese medicine gel of claim 1, its feature is that also its raw material medicines in portions by weight proportioning is:
Catechu 2-4 part, Radix Sophorae Flavescentis 8-18 part, Herba Menthae 4-8 part, Cortex Ailanthi 7-13 part, Fructus Cnidii 4-8 part.
3, Chinese medicine gel according to claim 1 is characterized in that, its preparation method may further comprise the steps:
(1) extraction process
Get Herba Menthae, extract volatile oil, get Herba Menthae Haplocalycis volatile oil with steam distillation; Get catechu 40%-80% alcohol reflux three times, each 1 hour, merge ethanol extract, decompression recycling ethanol also concentrates, and vacuum drying gets Catechu extract; Get Radix Sophorae Flavescentis, Cortex Ailanthi decocts with water secondary, 2 hours for the first time, 1 hour for the second time, merges decoction liquor, be concentrated into 2g crude drug/ml, concentrated solution adds ethanol to be made and contains alcohol amount and reach 60%, leaves standstill, filter, decompression filtrate recycling ethanol also concentrates, and gets Radix Sophorae Flavescentis, Cortex Ailanthi extract;
(2) preparation consists of: Herba Menthae Haplocalycis volatile oil 0.3-0.7 part, Catechu extract 12-24 part, extract 16-30 part of Radix Sophorae Flavescentis, Cortex Ailanthi, substrate 0.5-4 part, transdermal enhancer 0.5-1 part, wetting agent 5-20 part, solvent 34.5-51.5 part;
(3) formulation preparation
Get substrate, transdermal enhancer, add water-soluble expanding, the extract of getting Radix Sophorae Flavescentis, Cortex Ailanthi adds the water heating for dissolving, add in the substrate, it is standby to stir, and gets Catechu extract, Herba Menthae Haplocalycis volatile oil dissolves with glycerite, under agitation dropwise add in the above-mentioned substrate, stir promptly.
4, Chinese medicine gel according to claim 3, it is characterized in that substrate wherein is a kind of in carbomer, polyacrylamide, cross linked sodium polyacrylate, methylcellulose, sodium carboxymethyl cellulose, hydroxypropyl emthylcellulose, tragcanth, xanthan gum, the alginic acid.
5, Chinese medicine gel according to claim 3 is characterized in that, substrate weight percentage wherein is 0.5%-4%.
6, Chinese medicine gel according to claim 3 is characterized in that, transdermal enhancer wherein is the azone of weight percentage 0.5-1%.
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| CN114470089A (en) * | 2016-11-24 | 2022-05-13 | 东莞市乐堂医疗投资有限公司 | Externally applied Chinese medicine gel for treating colpitis and its prepn and application |
| CN107149569A (en) * | 2017-03-27 | 2017-09-12 | 广西厚德大健康产业股份有限公司 | A kind of antipruritic skin gels composition |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1080866A (en) * | 1993-02-26 | 1994-01-19 | 冯铃岚 | Powder medicine for wound and ulcer and manufacture method thereof |
| CN1368325A (en) * | 2001-02-05 | 2002-09-11 | 杨孟君 | Nano medicine 'Zhimiling' and its preparing process |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1080866A (en) * | 1993-02-26 | 1994-01-19 | 冯铃岚 | Powder medicine for wound and ulcer and manufacture method thereof |
| CN1368325A (en) * | 2001-02-05 | 2002-09-11 | 杨孟君 | Nano medicine 'Zhimiling' and its preparing process |
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