CN1293368A - Biological sensor of oxygen amount needed biochemically - Google Patents
Biological sensor of oxygen amount needed biochemically Download PDFInfo
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- CN1293368A CN1293368A CN 00132125 CN00132125A CN1293368A CN 1293368 A CN1293368 A CN 1293368A CN 00132125 CN00132125 CN 00132125 CN 00132125 A CN00132125 A CN 00132125A CN 1293368 A CN1293368 A CN 1293368A
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- measuring chamber
- dissolved oxygen
- bod
- measuring
- biochemically
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 38
- 239000001301 oxygen Substances 0.000 title claims abstract description 38
- 239000007853 buffer solution Substances 0.000 claims abstract description 7
- 210000001822 immobilized cell Anatomy 0.000 claims abstract description 4
- 238000005273 aeration Methods 0.000 claims description 16
- 238000012546 transfer Methods 0.000 abstract description 6
- -1 immobilized cells Substances 0.000 abstract description 4
- 238000005276 aerator Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 238000005259 measurement Methods 0.000 description 12
- 244000005700 microbiome Species 0.000 description 10
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 238000012544 monitoring process Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 229920002451 polyvinyl alcohol Polymers 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000003911 water pollution Methods 0.000 description 3
- 241000223233 Cutaneotrichosporon cutaneum Species 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229940058401 polytetrafluoroethylene Drugs 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical class OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A biosensor for oxygen amount needed biochemically is composed of measuring chamber containing buffer solution, immobilized cells, oxygen dissolving electrod in the center of measuring chamber, magnetic agitator, magnetic stirrer at bottom of measuring chamber, and micro aerator and aerating tube fixed near said electrode. Its advantages include low cost, improved mass transfer, wide linear range and high repeatability.
Description
The invention belongs to the environmental monitoring technology field, particularly the structural design of Water Pollution Monitoring mesophytization oxygen demand (BOD) fast measuring analytical instrument.
Sensor is can experience the specified measuring range material and convert it into the device or the device of available signal (mainly being electric signal) according to certain rule, and it is made up of sensitive element, conversion element and corresponding physical construction and electronic circuit usually.Wherein using biological component (enzyme, antibody, antigen, hormone) or biosome itself (cell, organelle, tissue) is biology sensor as the scales of sensitive element.
The biology sensor that carries out Water Pollution Monitoring mesophytization oxygen demand (BOD) fast measuring mainly is made of microorganism and oxygen electrode.Its measuring principle is: when not having organic aerobic substance in the measuring chamber, oxygen electrode is exported a steady state voltage, the endogenous respiration level of this steady state voltage representative sensor microorganism.After adding the mensuration water sample, existing biodegradable organism makes microorganism transfer the external source breathing rapidly in the water sample, consumes dissolved oxygen DO, and the oxygen electrode output voltage drops to another low-level stable state thereupon.Become linear dependence within the specific limits between two stable state differences and the detected water sample BOD concentration, thereby can carry out the quick measurement of BOD by this.
BOD biology sensor typical types is a sandwich method.Its structure is that the perforated membrane (14) that will be adsorbed with microorganism (13) is fixed on the surface of oxygen electrode (11) poly tetrafluoroethylene (12) with O type ring (15) as shown in Figure 1.Like this, microorganism just is sandwiched between perforated membrane (14) and poly tetrafluoroethylene (12) two membranes, so be referred to as sandwich method.
This structure exists the problem of following two aspects:
One, what oxygen electrode was measured in the sensor is not real dissolved oxygen content in the test water sample, but diffuses through biomembranous oxygen amount, makes electric signal weaken 3-6 the order of magnitude.So this instrument not only needs good measurement environment, and the support of the split hair dissolved oxygen DO instrument of needs, this just makes the price of integral sensors rise, and reaches more than 10,000 dollars.
They are two years old, the measurement range of this BOD determining instrument not only is subjected to the restriction of microorganism self metabolism, and controlled by the mass transfer of the perforated membrane of imbedded microbe, it is low to cause measuring linear range, generally only can arrive tens mg/litre, be lower than the BOD level of municipal effluent, measure again after can only diluting.
The objective of the invention is for overcoming the weak point of prior art, a kind of biochemical oxygen demand (BOD) biology sensor is proposed, it is low that it is had the dependence of high precision dissolved oxygen instrument, price is low, can improve mass transfer, enlarge the linear measurement scope, biological sensitive materials can be produced by batch, and reusable, can be used for the field survey requirement and can improve the advantages such as reappearance of measurement.
A kind of BOD biology sensor that the present invention proposes, it constitutes as shown in Figure 2, by uncovered measuring chamber (26), immobilized cell (27), dissolved oxygen electrode (21), magnetic stirrer (29) and magnetic stick (28), limited aeration device (211) is formed with aeration tube (210).Wherein, magnetic stick is placed on the measuring chamber bottom; The aeration flexible pipe of limited aeration device is fixed on the dissolved oxygen electrode next door; Dissolved oxygen electrode places measuring chamber central authorities; When each measurement begins, the buffer solution of certain volume all is housed in the measuring chamber, this plays the diluting effect to water sample to be measured, and can regulate the pH value, and microorganism is played a protective role.Buffer solution can adopt water or the aqueous solution that does not have organic pollution materials and bio-toxicity material to exist, for example aqueous phosphatic, deionized water, distilled water etc.
The present invention also can comprise a thermostat, and said measuring chamber places this thermostat.The effect of thermostat is to guarantee to measure to carry out under a stable temperature, improves measuring stability.
Characteristics of the present invention and effect
Principal feature of the present invention is to adopt the mixed solution of immobilization microorganism particles and buffer solution or buffer solution and water sample to be measured to reach rapid mass transfer and dissolved oxygen DO balance by magnetic agitation and limited aeration in sensor.This measuring method has been improved the weak point of original sensor, and has reached good effect.
The present invention has the dependence of high precision dissolved oxygen instrument low, and price is low, can improve mass transfer, enlarges the linear measurement scope, and biological sensitive materials can be produced by batch, and reusable, can be used for the field survey requirement and can improve the advantages such as reappearance of measurement.
Experiment shows that measuring the typical curve that draws has good linear dependence, for one group 0 ~ 200 BOD standard solution, this measuring method coefficient R
2Can reach more than 0.9.
This method also has good reappearance.For the standard solution of BOD50mg/L, day measurements 8 times, maximum relative deviation is 10.7%, less than the relative deviation of 5 days biochemical oxygen demand standard dilution meterings 15%.
Brief Description Of Drawings
Fig. 1 sandwich method BOD sensor construction synoptic diagram.
Fig. 2 BOD sensor construction of the present invention synoptic diagram.
Fig. 3 BOD sensor of the present invention example structure synoptic diagram.
A kind of biochemical oxygen demand (BOD) biology sensor embodiment that the present invention proposes, and with this sensor be the Water Pollution Monitoring system that forms of core as shown in Figure 3, form by (BOD) sensor and data recording and processing unit.Wherein, biochemical oxygen demand (BOD) biology sensor comprises: constant temperature water bath apparatus (312) be placed on TB-600 gradient stirrer (39) above, measuring chamber (36) (volume is 150ml) is placed in the water-bath device, and magnetic stick (38) is placed on the measuring chamber bottom.The aeration flexible pipe (310) of CTB-208 limited aeration pump (311) by adhesive securement on dissolved oxygen electrode (31) next door.Dissolved oxygen electrode places measuring chamber central authorities.
When each measurement begins, the buffer solution of certain volume all is housed in the measuring chamber, this plays the diluting effect to water sample to be measured, and can regulate the pH value, and microorganism is played a protective role.The effect of constant temperature water bath apparatus is to guarantee to measure to carry out under a stable temperature, improves measuring stability.Magnetic stirrer and limited aeration device are the mixing that is used for measuring chamber solution, reduce Measuring Time.
The data recording and processing unit mainly is made up of YSI-58 dissolved oxygen meter (313) and data collecting card (314).YSI-58 dissolved oxygen meter (313) links to each other with dissolved oxygen electrode (31), receives the electric signal of dissolved oxygen electrode output, and the dissolved oxygen DO that can monitor water body in the measuring chamber changes, and it changes the BOD value that just can characterize water sample.Data collecting card (314) is gathered the electric signal that dissolved oxygen meter (313) is exported, and it is stored in the computing machine, uses for subsequent treatment.And have the required program of data processing in the computing machine, the data reduction of gathering can be become measured BOD value.
Carry out the mensuration of the BOD concentration of urnormal with this experimental provision.
Experimental procedure:
At first carry out the polyvinyl alcohol gel embedding of microorganism trichosporon cutaneum (Trichosporon cutaneum).Wet cell weight 5g is suspended among the 5mL 0.9%NaCl, mixes mutually with the polyvinyl alcohol (PVA) of 12.5mL 10% and 1% mixed solution of sodium alginate.Syringe with 0.40mm ID is added drop-wise to the saturated boric acid of cooling and 1% CaCl then
2Mixed solution solution in, make its white ball-type globule that forms the 2mm diameter, and soak 24h therein, wash with physiological saline then.Said ratio can guarantee that immobilization particle can bear the stirring of long-time certain intensity, satisfies reusable requirement.This immobilized cell can be preserved dry back, puts it into before the use that the aeration activation promptly can be used for measuring in 24 hours in the nutrient solution, produces in batches and the requirement of field survey thereby satisfy.
Immobilization particle and 100mL deionized water mixed liquor are placed in the measurement cell of uncovered 150mL, stir and aeration with magnetic stick and limited aeration pipe, aeration intensity is 24L/h.The temperature of mixed liquor is set at 25 ℃, and pH is 5, treats wherein to add 10mL solution to be measured behind the dissolved oxygen stable, reduces to a new steady state (SS) until dissolved oxygen DO.Measuring Time is 15 minutes, and be 30 minutes release time.
The BOD concentration that this group is measured is 5,10,15,20,30,40,50, and measuring the linear related coefficient that is reached is 0.94, has satisfied the requirement of actual seawater on-line measurement.
Claims (2)
1, a kind of BOD biology sensor is characterized in that, by uncovered measuring chamber (26), and immobilized cell (27), dissolved oxygen electrode (21), magnetic stirrer (29) and magnetic stick (28), limited aeration device (211) is formed with aeration tube (210); Wherein, magnetic stick is placed on the measuring chamber bottom; The aeration tube of limited aeration device is fixed on the dissolved oxygen electrode next door; Dissolved oxygen electrode places measuring chamber central authorities; Buffer solution is housed in the measuring chamber.
2, BOD biology sensor as claimed in claim 1 is characterized in that, also comprises a thermostat, and said measuring chamber places this thermostat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001321250A CN1138141C (en) | 2000-12-15 | 2000-12-15 | Biological sensor of oxygen amount needed biochemically |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001321250A CN1138141C (en) | 2000-12-15 | 2000-12-15 | Biological sensor of oxygen amount needed biochemically |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1293368A true CN1293368A (en) | 2001-05-02 |
| CN1138141C CN1138141C (en) | 2004-02-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001321250A Expired - Lifetime CN1138141C (en) | 2000-12-15 | 2000-12-15 | Biological sensor of oxygen amount needed biochemically |
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| Country | Link |
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| CN (1) | CN1138141C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101819213A (en) * | 2010-05-07 | 2010-09-01 | 北京航空航天大学 | Method for quantitatively testing activity of microalgae |
| CN101261242B (en) * | 2008-04-21 | 2010-12-22 | 同济大学 | Water quality toxicity biosensor microorganism electrode preparation method |
| WO2011022895A1 (en) * | 2009-08-31 | 2011-03-03 | 清华大学 | Rapid measurement method for biochemical oxygen demand (bod) by using saccharomyces cerevisiae as biological recognition elements |
| WO2011022921A1 (en) * | 2009-08-31 | 2011-03-03 | 清华大学 | Reactor-type rapid measuring instrument for biochemical oxygen demand (bod) and measurement method thereof |
| CN101644702B (en) * | 2009-09-02 | 2014-07-23 | 清华大学 | Method for rapid BOD determination using Saccharomyces cerevisiae as biometric elements |
-
2000
- 2000-12-15 CN CNB001321250A patent/CN1138141C/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101261242B (en) * | 2008-04-21 | 2010-12-22 | 同济大学 | Water quality toxicity biosensor microorganism electrode preparation method |
| WO2011022895A1 (en) * | 2009-08-31 | 2011-03-03 | 清华大学 | Rapid measurement method for biochemical oxygen demand (bod) by using saccharomyces cerevisiae as biological recognition elements |
| WO2011022921A1 (en) * | 2009-08-31 | 2011-03-03 | 清华大学 | Reactor-type rapid measuring instrument for biochemical oxygen demand (bod) and measurement method thereof |
| CN101644702B (en) * | 2009-09-02 | 2014-07-23 | 清华大学 | Method for rapid BOD determination using Saccharomyces cerevisiae as biometric elements |
| CN101819213A (en) * | 2010-05-07 | 2010-09-01 | 北京航空航天大学 | Method for quantitatively testing activity of microalgae |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1138141C (en) | 2004-02-11 |
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Assignee: Jiangsu Tonghe Painting Machinery Co., Ltd. Assignor: Tsinghua University Contract fulfillment period: 2008.12.20 to 2015.12.19 Contract record no.: 2009990000072 Denomination of invention: Biological sensor of oxygen amount needed biochemically Granted publication date: 20040211 License type: exclusive license Record date: 20090209 |
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| LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.12.20 TO 2015.12.19; CHANGE OF CONTRACT Name of requester: JIANGSU TONGHE PAINTING MECHANICAL CO., LTD. Effective date: 20090209 |
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| CX01 | Expiry of patent term |
Granted publication date: 20040211 |