CN1291076A

CN1291076A - Use of neonicodinoids on transgenic plants

Info

Publication number: CN1291076A
Application number: CN99802979A
Authority: CN
Inventors: B·李; M·苏特; H·布霍尔泽
Original assignee: Novartis AG
Current assignee: Syngenta Participations AG
Priority date: 1998-01-16
Filing date: 1999-01-14
Publication date: 2001-04-11
Anticipated expiration: 2019-01-14
Also published as: MX233459B; AU2517699A; KR100701817B1; JP2002509865A; KR20060029293A; WO1999035913A1; BRPI9917838B1; AR018264A1; ES2181568A1; HUP0100528A2; AU744003B2; BR9907010B1; KR100593584B1; FR2773672A1; AR076816A2; CA2317050C; CN1220435C; CA2317050A1; HU230681B1; GB0017168D0

Abstract

There is now described a method of controlling pests with nitroimino- or nitroguanidino-compounds; more specifically a method of controlling pests in and on transgenic crops of useful plants, such as, for example, in crops of maize, cereals, soya beans, tomatoes, cotton, potatoes, rice and mustard, with a nitroimino- or nitroguanidino-compound, especially with thiamethoxam, characterized in that a pesticidal composition comprising a nitroimino- or nitroguanidino-compound in free form or in agrochemically useful salt form and at least one auxiliary is applied to the pests or their environment, in particular to the crop plant itself.

Description

The class anabasine is to the application of genetically modified plants

The present invention relates to a kind of with the nitro imino group-or nitro guanidine radicals-compound particularly kill the method for (thiamethoxam) pest control with the thiophene first; More specifically, relate to a kind of with the nitro imino group-nitro guanidine radicals-compound control among the genetically modified crops of useful plants or on the new method of insect.

Some insect pest control methods had once been proposed in the literature.Yet these methods can not make us fully satisfied in field of pest control, therefore need provide better method to come pest control, and especially insect and Acarina is representative biological, or are used for protective plant, especially crop.Can realize this purpose according to method provided by the invention.

Therefore the present invention relates to a kind of method of preventing and treating the genetically modified crops such as the insect in the crops such as corn, cereal, soybean, tomato, cotton, potato, paddy rice and leaf mustard of useful plants, it is characterized in that especially using a kind of Pesticidal combination to crop itself to insect or its environment, it contains the nitro imino group of salt form useful on a kind of free form or the agrochemistry-or nitro guanidine radicals-compound, particularly the thiophene first kill, Imidacloprid, Ti-435 or thiacloprid and at least a auxiliary agent; The propagating materials of the genetically modified plants that also relate to the purposes of described composition and handled with said composition.

Surprisingly have now found that, express desinsections and especially kill on the transgenosis useful plants of insect, the gene that kills mite, nematicide or Fungicidal active ingredient or herbicide-tolerant or the infringement of tolerance fungi for example containing one or more, with the nitro imino group-or the application of nitro guanidine radicals-compound pest control have synergy.Very surprisingly, the nitro imino group-or the use in conjunction of nitro guanidine radicals-compound and genetically modified plants can be above the addition to the desire pest control of expecting in principle, therefore enlarge nitro imino group-or the sphere of action of the active ingredient expressed of nitro guanidine radicals-compound and genetically modified plants, especially shown following two aspects:

Particularly, now shockingly find, within the scope of the invention, the nitro imino group-or the insecticidal activity of nitro guanidine radicals-compound combine with the effect that the transgenosis useful plants is expressed, with independent nitro imino group-or the insecticidal activity of nitro guanidine radicals-compound and independent genetically modified plants compare, the addition that common expection is not only arranged, and have synergy.Yet term " is worked in coordination with " never to should be understood in this manual and is only limited to insecticidal activity, this term also refer to independent nitro imino group-or nitro guanidine radicals-compound compare the favourable character of other of the method according to this invention with the transgenosis useful plants.The example of these the favourable character that can mention is: to the expansion of the insecticidal spectrum of other insects such as resistant strain effect; The nitro imino group-or the minimizing of nitro guanidine radicals-compound administration amount, perhaps in addition independent nitro imino group-or the invalid fully amount of application of nitro guanidine radicals-compound and independent transgenosis useful plants under, by means of the effective control of composition according to the present invention to insect; The crop safety that improves; The product quality that improves is as the content of any residue of the content of the toxic products of the storage time of higher nutrient or oil content, fiber quality, prolongation preferably, reduction such as mycotoxin, reduction or unfavorable composition or better digestibility; The tolerance that improves to salt content in unfavorable temperature, arid or the water; Assimilability that improves such as nutrient uptake, moisture picked-up and photosynthesis; Good crop character is as the output of nourishing and growing, increasing of the leaf area that changes, reduction, good shape of the seed/seed density or sprout the saprophyte of characteristic, change or the cotton boll of the increase (flower set increase) of cluster that epiphyte causes, the aging phytoalexin output that delays, improves, the hasting of maturity of promoting, flower, minimizing comes off and cracked, the better attraction to beneficial organism and predator, the pollination of increase, the attraction to bird of reduction; Or other benefits known to those skilled in the art.

The nitro imino group-and nitro guanidine radicals-compound, kill (5-(2-diuril azoles-5 bases-methyl)-3-methyl-4-nitro imino group-perhydro--carotene 1,3,5-oxadiazine) as the thiophene first, know by EP-A-0 ' 580 ' 553.Within the scope of the invention, the thiophene first is preferred extremely.

General formula within the scope of the invention

Imidacloprid also be preferred, see " agricultural chemicals handbook (The Pesticide Manual), the 10th edition (1991), the Britain crop protection committee, London, the 591st page;

General formula Thiacloprid also be preferred, see EP-A-235 ' 725;

General formula

Compound also be preferred, be called Ti-435 (Clothiamidin) among the EP-A-376 ' 279.The nitro imino group-or the agrochemistry of nitro guanidine radicals-compound on the salt that is fit to be, for example inorganic acid and organic acid acid-addition salts, especially hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, perchloric acid, phosphoric acid, formic acid, acetate, trifluoroacetic acid, oxalic acid, malonic acid, toluenesulfonic acid or benzoic acid.Preferably a kind of within the scope of the present invention known composition own, its thiophene first that comprises free form is killed and Imidacloprid, especially thiophene first are killed as active ingredient.

Genetically modified plants used according to the invention are to utilize recombinant DNA technology plant transformed or its propagating materials, make their for example synthetic effect of selectivity toxin like this, known these toxin sources are in for example toxin producing invertebrate, especially Arthropoda (Arthropoda) can obtain from Su Yun gold gemma bar mattress (Bacillus thringiensis) bacterial strain; Or knownly derive from plant, as agglutinin; Perhaps can express weed killer herbicide or fungicide resistance in addition.The example that these toxin maybe can synthesize the genetically modified plants of these toxin is disclosed in, and for example EP-A-0 374753, WO 93/07278, WO 95/34656, EP-A-0 427 529 and EP-A-451 878 quote as a reference in this application.

The method that produces this genetically modified plants is known by those skilled in the art, and is for example stating in the above-mentioned publication.

Can comprise by the toxin that these genetically modified plants are expressed, toxin for example, as has insecticidal property and by the genetically modified plants expressed protein, for example bacillus cereus (Bacillus cereus) albumen or Japanese beetle bacillus (Bacillus popliae) albumen; Or bacillus thuringiensis endotoxin (B.t.), as Cry I A (a), Cry I A (b), Cry I A (c), Cry II A, Cry III A, Cry III B2 or CytA; VIP1; VIP2; VIP3; Or the kind of bacterium of gathering nematode such as polished rod shape Pseudomonas (Photorhabdus) or the kind of Xenorhabdus genus (Xenorhabdus) such as the insect-killing protein of luminous smooth rod bacterium (Photorhabdus luminescens), Xenorhabdus nematophilus (Xenorhabdusnematophilus) etc.; Protease inhibitors is as trypsin inhibitor, serpin, patatin, cystatin, antipain; Ribosome inactivating protein (RIP) is as ricin, corn RIP, abrin, luffin, saporin or bryodin; Phytolectin such as pisum sativum agglutinin, barley lectin element or Galanthus Nivalis Agglutinin (GNA); Or acrasin (agglutinin); The toxin that animal produces is as scorpion toxin, spider venom, wasp venom and the distinctive neurotoxin of other insects; The steroid metabolism enzyme, as 3-hydroxy steroid oxidase, ecdysteroid UDP-glycosyl transferase, cholesterol oxidase, moulting hormone inhibiting factor, HMG-CoA reductase, ion channel blocking agent such as sodium and potassium, juvenile hormone esterase, diuretic hormone acceptor, 1,2-two-styrene synthase, bibenzyl synthase, chitinase and dextranase.

Comprise one or more coded insect-killing agent resistances and express the example of known genetically modified plants of gene of one or more toxin as follows: KnockOut ^(corn), YieldGard ^(corn); NuCOTN33B ^(cotton), Bollgard ^(cotton), NewLeaf ^(potato), NatureGard ^And Protecta ^

Following table has comprised the target of genetically modified crops, the composition of expression and more examples of crop phenotype, these genetically modified crops mainly are insect, mite, nematode, virus, bacterium and disease performance tolerance to insect, perhaps tolerate specific weed killer herbicide or weed killer herbicide kind.

Table A 1: crop: corn

Table A 2: crop: wheat

Table A 3: crop: barley

Table A 4: crop: paddy rice

Table A 5: crop: soybean

Table A 6: crop: potato

Table A 7: crop: tomato

Table A 8: crop: pepper

Table A 9: crop: grape

Table A 10: crop: oilseed rape

Table A 11: crop: brassica vegetables (wild cabbage, brussels sprouts (Brussel sprouts), sprouting broccoli etc.)

Table A 12: crop: pomaceous fruits is apple, pears for example

Table A 13: crop: muskmelon class

Table A 14: crop: banana

Table A 15: crop: cotton

Table A 16: crop: sugarcane

Table A 17: crop: sunflower

Table A 18: crop: beet, beet root

The above-mentioned animal class pest of available the present invention's (A) method control comprises that for example representative biology of insect, Acarina and Nematoda is representative biological; Lepidoptera particularly: Acleris spp belongs to the kind of (Acleris), the kind, particularly adoxophyes moth (Adoxophyes reticulana) that Adoxophyes spp belongs to (Adoxophyes); Clearwing moth belong to (Aegeria) kind, tiger belong to the kind of (Agrotis), particularly little sword ground noctuid (Agrotis spinifera); Cotton leaf noctuid (Alabama argillaceae), the kind of Amylois, soybean noctuid (Anticarsiagemmatalis), the kind of Archips spp (Archips), Argyrotaenia spp belongs to the kind of (Argyrotaenia), gamma belongs to the kind of (Autographa), the dried noctuid of corn (Busseola fusca), Cadra cautella (Cadra cautella), peach fruit moth (Carposina nipponensis), the kind of straw borer spp (Chilo), Choristoneura spp belongs to the kind of (Choristoneura), grape codling moth (Clysia ambiguella) (Clysia ambiguella), Cnaphalocrocis spp belongs to the kind of (Cnaphalocrocis), cloud volume moth belongs to the kind of (Cnephasia), Cochylisspp belongs to the kind of (Cochylis), the kind of casebearer moth (Coleophora), general non-fine hair snout moth's larva (Crocidolomia binotalis), the special-shaped steinernema (Cryptophlebia leucotreta) of peach, steinernema belongs to the kind, particularly codling moth (Cydia pomonella) of (Cydia); Kind, the Sudan cotton bollworm (Diparopsis castanea), Jin Gang that crambid belongs to (Diatraea) bore the kind that belongs to (Earias), the kind, particularly Anagasta kuehniella (E.Khuniella) that meal moth belongs to (Ephestia); The real noctuid (Heliothis zea) of the kind, particularly tobacco budworm (Heliothis virescens) of the kind of the kind of flower steinernema genus (Eucosma), ligustrum fine tortricidae (Eupoecilia ambiguella), the kind of Euproctis (Euproctis), the kind of cutting Noctua (Euxoa), Grapholita spp genus (Grapholita), green wide wing steinernema (Hedya nubiferana), Heliothis (Heliothis) and paddy; Hellula undalis (Hellula undalis), fall webworm (Hyphantria cunea), tomato stem gelechiid (Keiferia lycopersicella), pear leaf blister moth (Leucoptera scitella), thin moth belongs to the kind of (Lithocollethis), the little moth of flower wing belongs to the kind of (Lobesia), the kind of Euproctis (Lymantria), lyonetid belongs to the kind of (Lyonetia), the kind of Malacosoma (Malacosoma), tomato moth (Mamestra brassicae), maduca sexta (Manduca sexta), the kind of autumn geometrid moth genus (Operophtera), European corn borer (Ostrinia nubilalis), super steinernema belongs to the kind of (Pammene), the kind of brown epiblema (Pandemis), small noctuid (Panolisflammea), Pectinophora gossypiella belongs to the kind of (Pectinophora), tobacco split worm (Phthorimaeaoperculella), imported cabbageworm (Pieris rapae), the kind of Pier (Pieris), diamond-back moth (Plutella xylostella), the kind of Yponomeuta (Prays), white standing grain snout moth's larva belongs to the kind of (Scirpophaga), the kind of moth stem Noctua (Sesamia), the long kind that must roll up moth genus (Sparganothis), Spodoptera littoralis (Spodoptera littoralis), emerging clearwing moth belongs to the kind of (Synanthedon), the kind of Thaumetopoea (Thaumetopoea), the volume moth belongs to the kind of (Tortrix), the kind of amyloid plaque noctuid (Trichoplusia ni) and Yponomeuta (Yponomeuta);

Coleoptera, for example kind of Agriotes spp (Agriotes), flower resembles the kind of genus (Anthonomus), Atomaria linearis (Atomaria linearis), beet shin flea beetle (Chaetocnema tibialis), collar resembles the kind of genus (Cosmopolites), the kind of Curculio (Curculio), khapra beetle belongs to the kind of (Dermestes), the asterophyllite first belongs to the kind of (Diabrotica), the kind of epilachna genus (Epilachna), the kind of Eremnus, colorado potato beetles (Leptinotarsa decemlineata), rice water resembles the kind of genus (Lissorhoptrus), gill cockchafer belongs to the kind of (Melolontha), saw-toothed grain beetle belongs to the kind of (Oryzaephilus), the ear beak resembles the kind of genus (Otiorhynchus), spot resembles the kind of genus (Phlyctinus), the arc rutelian belongs to the kind of (Popillia), the kind of Psylliodes spp (Psylliodes), the long moth-eaten kind that belongs to (Rhizopertha) of moth, Scarabaeidae (Scarabaeidae), the kind of Sitophilus (Sitophilus), gelechiid belongs to the kind of (Sitotroga), mealworm belongs to the kind of (Tenebno), the kind that the kind of Tribolium (Tribolium) and spot khapra beetle belong to;

Orthoptera, for example the roach sickle belongs to the kind of (Blatta), the kind of Blatella (Blattella), kind, Madeira roach sickle (Leucophaea maderae), the kind of migratory locusts genus (Locusta), the kind of Periplaneta (Periplaneta) and the kind that desert locust belongs to (Schistocerca) of Gryllotalpa spp (Gryllotalpa);

Isoptera, for example kind of Reticulitermes (Reticulitermes);

Corrodentia, for example powder corrodent belongs to the kind of (Liposcellis); Anoplura, for example the kind of Haematopinus (Haematopinus), jaw lice belong to the kind of (Linognathus), kind, the goitre woolly aphid that lice belongs to (Pediculus) belongs to the kind of (Pemphigus) and the kind of Phylloxera spp (Phylloxera);

Mallophaga, for example kind of the kind of Damalinia (Damalinea) and Trichodectes (Trichodectes);

Thrips, for example flower thrips belongs to kind, the kind of hedge Thrips (Hercinothrips), the kind of Taeniothrips (Taeniothrips), joint melon thrips (Thrips palmi), onion thrips (Thrips tabaci) and the orange thrips in South Africa (Scirtothrips aurantii) of (Frankliniella); Heteroptera, for example the kind of the kind of the kind of Cimex (Cimex), cocoa Di fleahopper (Distantiellatheobroma), red cotton bug genus (Dysdercus), Euchistus, Eurygasterspp belong to the kind of (Eurygaster), the kind that Leptocorisa spp belongs to (Leptocorisa), the kind of Bemisia spp (Nezara), the kind that the skin stinkbug belongs to (Piesma), the kind of Rhodnius (Rhodnius), the kind of Adelphocoris (Sahlbergella), the kind of black stinkbug genus (Scotinophara) and the kind of vertebra Reduvius (Triatoma);

Homoptera, for example wolly whitefly (Aleurothrixus floccosus), Aleyrodesbrassicae, red kidney Aspidiotus (Aonidiella aurantii), Aphidiadae (Aphididae), bean aphid (Aphis craccivora), aphis fabae (A.fabae), cotten aphid (A.gosypii); Aspidiotus belongs to the kind of (Aspidiotus), sweet potato whitefly (Bemisia tabaci), lecanium belongs to the kind of (Ceroplaster), dark brown Aspidiotus (Chrysomphalus aonidium), Chrysomphalus ficus (Chrysomphalus dictyospermi), brown soft wax a red-spotted lizard (Coccus hesperidum), green jassids belongs to the kind of (Empoasca), wooly aphis (Eriosoma lanigerum), Erythroneura spp belongs to the kind of (Erythroneura), the kind of Gascardia, small brown rice planthopper belongs to the kind of (Laodelphax), Europe fruit hard ball a red-spotted lizard (Lecanium corni), the kind of lepidosaphes shimer (Lepidosaphes), the kind of long tube Aphis (Macrosiphus), the kind, particularly black peach aphid (M.persicae) of tumor aphid genus (Myzus); Rice leafhopper belongs to the kind, particularly rice leafhopper (N.cincticeps) of (Nephotettix); Brown planthopper belongs to the kind, particularly brown plant-hopper (N.lugens) of (Nilaparvata); The kind of the kind of ring wing volume moth genus (Paratoria), goitre woolly aphid genus (Pemphigus), stern line mealybug belong to the kind of (Planococcus), white armored scale belongs to the kind of (Pseudaulacaspis), kind, particularly star mealybug (Pseudococcus fragilis), the little mealybug of tangerine (Pseudococcuscitriculus) and the Kang Shi mealybug (Pseudococcus comstocki) of mealybug genus (Pseudococcus); The kind, particularly pear sucker (P.pyri) of Psylla spp (Psylla); Pulvinaria aethiopica, large bamboo hat with a conical crown and broad brim Aspidiotus belong to the kind of (Quadraspidiotus), the kind of Rhopalosiphum (Rhopalosiphum), the kind that the pearl lecanium belongs to (Saissetia), the kind that the band leafhopper belongs to (Scaphoideus), the kind of y-bend Aphis (Schizaphis), kind, greenhouse whitefly (Trialeurodesvaporariorum), a mandarin orange wood louse (Trioza erytreae) and the citrus point armored scale (Unaspiscitri) of paddy net Aphis (Sitobion);

Hymenoptera, for example Acromyrmex, leaf cutting ant belong to kind, the kind of Solenopsis (Solenopsis) and the kind of Vespa (Vespa) that the kind of the kind of (Atta), kind that the stem honeybee belongs to (Cephus), the lucky pine sawfoy (Gilpinia polytoma) of kind, diprionidae (Diprionidae), dragon spruce that pine sawfoy belongs to (Diprion), real tenthredinidae (Hoplocampa), kind, MonomoriumMayr (Monomorium pharaonis), new pine sawfoy that the hair ant belongs to (Lasius) belong to (Neodiprion);

Diptera, for example kind of Aedes (Aedes), Antherigona soecata, garden march fly (Bibio hortulanus), calliphora erythrocephala (Calliphora erythrocephala), the kind of little Anastrepha (Ceratitis), the kind of Carysomyia (Chrysomyia), the kind of Culex (Culex), cuterbrid belongs to the kind of (Cuterebra), the kind of few hair on the neck Anastrepha (Dacus), black-tailed fruit flies (Drosophila melanogaster), the kind of Fannia (Fannia), the kind of Gasterophilus (Gastrophilus), the kind of Glossina (Glossina), the kind of Hypoderma (Hypoderma), the kind of Hyppobosca, liriomyza bryoniae belongs to the kind of (Liriomyza), the kind of Lucilia (Lucilia), the kind of black Hippelates (Melanagromyza), the kind of Musca (Musca), the kind of Oestrus (Oestrus), rice gall midge belongs to the kind of (Oreseolia), Europe wheat stem chloropid fly (Oscinella frit), henbane seed spring fly (Pegomyia hyoscyami), the kind of grass Hylemyia (Phorbia), apple is around trypetid (Rhagoletispomonella), mushroom fly belongs to the kind of (Sciara), the kind of Genus Stomoxys (Stomoxys), the kind of Gadfly (Tabanus), a smaller kind of cicada cicada belongs to the kind of (Tannia) and the kind of big uranotaenia (Tipula);

Siphonaptera, for example kind of Ceratophyllus (Ceratophyllus) and Xanthopsyllacheopis (Xenopsyllacheopis);

Thysanoptera, for example Taiwan silverfish (Lepisma saccharina) and

Acarina, for example Acarus siro (Acarus siro), tangerine bud goitre mite (Aceria sheldoni); The kind of thorn Eriophyes (Aculus), particularly Si Shi thorn goitre mite (A.schlechtendali); The kind of the kind of the kind of the kind of Amblyomma (Amblyomma), Argas (Argas), Boophilus (Boophilus), short whisker Acarapis (Brevipalpus), particularly California short hairs mite (Brevipalpuscalifornicus) and purplish red short hairs mite (Brevipalpus phoenicis); The kind of the kind of the kind of Bryobia praetiosa (Bryobiapraetiosa), Calipitrimerus, Psoroptes (Chorioptes), Dermanyssus gallinae (Dermanyssus galinae), beginning Tetranychus (Eotetranychus), particularly the carpinus turczaninowii beginning tetranychid (E.carpini) and Eotetranychus orientalis; Kind, the particularly grape erineum mite (Eriophyes vitis) (E.vitis) of Eriophyes (Eriophyes); The kind of the kind of the kind of Hyalomma (Hyalomma), hard tick genus (Ixodes), meadow unguiculus mite (Olygonychus pratensis), Ornithodorus (Ornithodoros), Panonychus citri belong to kind, particularly panonychus ulmi (P.ulmi) and the citrus tangerine Panonychus citri (P.citri) of (Panonychus); The wrinkle leaf Aculus belongs to the kind, particularly citrus rust mite (Phyllocoptruta oleivora) of (Phyllocoptruta); Many food tarsonemids belong to the kind, particularly Polyphagotarsonemus latus Banks (P.latus) of (Polyphagotarsonemus); The scabies mite belongs to the kind of (Psoroptes), the kind of Rh (Rhipicephalus), the kind that the root mite belongs to (Rhizoglyphus), kind, the kind of tarsonemid genus (Tarsonemus) and kind, particularly Tetranychus urticae (Tetranychus urticae) and the refreshing Ze Shi tetranychid (Tetranychus Kanzawai) of Tetranychus (Tetranychus) that itch mite belongs to (Sarcoptes);

The representative biology of Nematoda;

(1) is selected from the nematode that root-knot nematode, cyst form nematode, stem eelworm and leaf nematode;

(2) be selected from following nematode: the kind of eel Turbatrix (Anguina); The kind of Aphelenchoides (Aphelenchoides); The kind of Ditylenchus (Ditylenchus); The kind of ball Heterodera (Globodera), as globodera rostochiensis (Globodera rostochiensis); The kind of Heterodera (Heterodera) is as oat Cyst nematode (Heterodera avenae), soybean Cyst nematode (Heterodera glycines), beet Cyst nematode (Heterodera schachtii) or clover Cyst nematode (Heterodera trifolii); The kind of minute hand Turbatrix (Longidorus); The kind of Meloidogyne (Meloidogyne) is as Meloidogyne incognita (Meloidogyneincognita) or javanese root knot nematode (Meloidogyne javanica); Pratylenchidae belongs to the kind of (Pratylenchus), as unelected Pratylenchidae (Pratylenchus neglectans) or Cobb root (Pratylenchus penetrans); The kind of perforation line Eimeria (Radopholus) is as similar similes thorne (Radopholus similis); The kind of burr Turbatrix (Trichodorus); The kind of pulvinulus sword Turbatrix (Tylenchulus) is as the nematode of partly puncturing (Tylenchulussemipenetrans); And the kind of Xiphinema (Xiphinema); Or

(3) be selected from kind such as the kind of soybean Cyst nematode and Meloidogyne such as the nematode of Meloidogyne incognita of Heterodera.

The method according to this invention can be prevented and treated i.e. control (contained) or be eliminated the insect of the above-mentioned type, these insects especially appear on the genetically modified plants, mainly be useful plant and ornamental plants in agricultural, horticulture and forestry, perhaps appear on some part such as fruit, flower, leaf, stem, stem tuber or root of these plants, in some cases, to the protection of this insect even can expand to the plant part that formed afterwards.

Use the method according to this invention can effectively be prevented and treated the insect on the following plants: paddy rice, cereal such as corn or Chinese sorghum; Fruit, for example for example strawberry, raspberry and blackberry, blueberry of drupe, the operatic circle and fruitlet such as apple, pears, plum, peach, apricot, cherry or berry; Beans such as Kidney bean, French beans, pea or soybean; Oil crop such as oilseed rape, leaf mustard, opium poppy, olive, sunflower, coconut, castor-oil plant, cocoa or peanut; Curcurbitaceae such as pumpkin, cucumber or muskmelon; Fibre plant such as cotton, flax, hemp or jute; Cedra fruits is as orange, lemon, shaddock or red tangerine; The kind of vegetables such as spinach, lettuce, asparagus, wild cabbage, carrot, onion, tomato, potato, beet or capsicum; Lauraceae such as avocado, Cinnamomum (Cinnamonium) or camphor; Or tobacco, nut, coffee, eggplant, sugarcane, tea, pepper, grape, lupulus, banana section, rubber plant or ornamental plants, mainly be corn, paddy rice, cereal, soybean, tomato, cotton, potato, beet, paddy rice and leaf mustard; Particularly cotton, paddy rice, soybean, potato and corn.

Have now found that even when the Pesticidal combination of low working concentration, the method according to this invention also has prevention and/or cures value in field of pest control, therefore can obtain very satisfied insecticidal spectrum.Protect it not to be subjected to the kind of the genetically modified crops of insect infringement according to desire; can combine with the composition that is used for warm blooded animal kind, fish and plant with excellent compatibility; use the method according to this invention to prevent and treat all developmental stage or the normal susceptibility of indivedual developmental stage and the animal class pest of normal tolerance, as the representative biology of insect and barnyard grass Acarina.Desinsection of the method according to this invention and/or miticidal effect can become directly visible, insect destruction that promptly take place immediately or that only in the process of for example casting off a skin after after a while, just take place, perhaps become visible indirectly, for example spawning rate of Jiang Diing and/or incubation rate, corresponding to the good action of 40-50% destructive rate (lethality) at least.

According to intended purposes and common environment, but own the known insecticide in the scope of the invention is to contain nitro imino group-or the emulsifiable concentrates of nitro guanidine radicals-compound, concentrate suspension, directly can spray or dilutable solution, the paste that can be coated with, dilute emulsion, wetting powder, soluble powder dispersion powder, wetting powder, dust, granule or polymer latex wafer.

In these compositions, use active ingredient and at least a simultaneously at formulation art auxiliary agent commonly used, as swelling agent, for example solvent or solid carrier, or as surface active cpd (surfactant).

The formulation auxiliary agents that uses is, for example solid carrier, solvent, stabilizing agent, " slowly-releasing " auxiliary agent, colouring agent and the surface reactive material (surfactant) suitably the time.Suitable carriers and auxiliary agent are the materials that all routines are used for crop protection products.Proper auxiliary agent such as solvent, solid carrier, surface active cpd, nonionic surface active agent, cationic surfactant, anion surfactant and other auxiliary agents are in the composition used according to the invention, for example at those auxiliary agents described in the EP-A-736252.

These compositions that are used for pest control can be made into, for example wetting powder, dust, granule, solution, emulsifiable concentrates, concentrated suspension or aerosol.For example, composition is the type described in the EP-A-736 252.

By adding other desinsections, killing mite and/or antifungal active ingredient, can expand the scope of the invention greatly and contain nitro imino group-or the effect of nitro guanidine radicals-compound compositions, and make it adapt to common environment.The suitable example of the active ingredient of adding is the representative of following kind of effective constituents: organic phosphorus compound, nitrophenols and derivative, carbonamidine, urea, carbamate, pyrethroid, chlorinated hydrocabon; Particularly preferred composition is in the mixture, for example Avermectin, emamectin, spinosad, pyrrole aphid ketone, fenoxycarb, Ti-435, fluorine worm nitrile, pyrrole propyl ether, diazinon or butyl ether urea.

Usually, composition in the scope of the invention comprises the nitro imino group of 0.1-99%, particularly 0.1-95%-or nitro guanidine radicals-compound, at least a solid or liquid adjuvants with 1-99.9%, particularly 5-99.9%, the composition that 0-25%, particularly 0.1-20% are arranged usually may be surfactant (in all cases, % represents percentage by weight).Although the composition that concentrates is preferred as commodity, the end user can use dilute compositions usually, and it contains the active ingredient that concentration reduces greatly.

Also can comprise other solids or liquid adjuvants according to composition of the present invention, as stabilizing agent, for example epoxidation or not epoxidised vegetable oil (for example epoxidised cocoa butter, rapeseed oil or soya-bean oil), defoamer, silicone oil for example, preservative, viscosity modifier, adhesive and/or adhesive, other active ingredients that also have fertilizer or be used to reach special-effect are bactericide, fungicide, nematocide, invertebrate poison or weed killer herbicide for example.

Composition according to the present invention is to produce with known method, for example with before auxiliary agent mixes, grinds, screening and/or compress active ingredient, for example obtains specific particle size, and active ingredient closely mixed with auxiliary agent and/or grinds.

According to intended purposes and common environment; with the method according to this invention that the known method of those skilled in the art itself implements to be used to prevent and treat the above-mentioned type insect, that is to say and use to spray, moistening, spraying, dusting, brushing, seed dressing, disperse or pour into the method for said composition.General working concentration is 0.1-1000ppm, the active ingredient of 0.1-500ppm preferably.Amount of application can change in wide region, and depends on that soil constitutes, uses type (foliage applying; Seed dressing; Use in kind of the furrow), main weather conditions under the insect of genetically modified crops, desire control, every kind of situation, and other are by using type, using the factor that opportunity and target crop are determined.The amount of application of per hectare is generally per hectare 1-2000g nitro imino group-or nitro guanidine radicals-compound, particularly 10-1000g/ hectare, preferably 10-500g/ hectare, more preferably 10-200g/ hectare.

In the crop protection field within the scope of the present invention, preferably using type is to use (foliar spray is used) to the blade of plant, can regulate frequency and the consumption used, makes it adapt to the danger of being encroached on by described insect.Yet, soak the plant site or solid-state active ingredient mixed the plant site with fluid composition, for example mix (soil application) in the soil with the form of particle, active ingredient also can unite by root system (systemic action) enter inside plants.For rice crop, these particles can add in the paddy field.

Also be suitable for protecting the propagating materialss of genetically modified plants according to composition of the present invention, for example seed such as fruit, stem tuber or kernel, or plant transplants make it not to be subjected to the particularly representative biological infringement of insect and barnyard grass Acarina of noxious animal.Can before using, use the compositions-treated propagating materials, for example seed dressing prior to seeding.Also can by in fluid composition, soak kernel or with the solid composite bag by kernel, active ingredient is applied on kind of the benevolence (bag by).When in the sowing process, propagating materials being applied to when for example planting in the furrow, also composition can be applied to and use the place.These all are further themes of the present invention to the processing method of plant propagation material and the plant propagation material of so handling.

The nitro imino group that can use in the method according to the invention-or the formulation examples of nitro guanidine radicals-compound, for example solution, granule, dust, the pulvis that can spray, emulsifiable concentrates, coated granule and concentrated outstanding agent all are the types of describing in the embodiment F 1-F10 of for example EP-A-580 553.

Table B

Use following abbreviation in the table:

The active ingredient of genetically modified plants: AP

Luminous smooth rod bacterium: PL

Xenorhabdus nematophilus: XN

Protease inhibitors: Plnh.

Phytolectin: PLec.

Acrasin: Aggl.

3-hydroxy steroid oxidase: HO

Cholesterol oxidase: CO

Chitinase: CH

Dextranase: GL

1,2-talan synthase: SS

Table B:

Biology embodiment

Table 1: a kind of method of pest control comprises to transgene cotton and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 2: a kind of method of pest control comprises to transgenic paddy rice and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 3: a kind of method of pest control comprises to transgenic potato and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 4: a kind of method of pest control comprises to the transgenosis rape and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 5: a kind of method of pest control comprises to transgene tomato and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 6: a kind of method of pest control comprises to the transgenosis cucurbit and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 7: a kind of method of pest control comprises to genetically engineered soybean and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 8: a kind of method of pest control comprises to transgenic corns and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 9: a kind of method of pest control, comprise that using the thiophene first to transgenic wheat kills, the combination of the insect of active ingredient that genetically modified plants are wherein expressed and pre-control is corresponding to the v of table B.

Table 10: a kind of method of pest control comprises to the transgenosis banana and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 11: a kind of method of pest control comprises to the transgenosis orange tree and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 12: a kind of method of pest control, comprise that using the thiophene first to transgenosis pomaceous fruits tree kills, wherein the combination of the insect of the active ingredient expressed of genetically modified plants and desire control is corresponding in each combination B.1-B.1170 of table B any.

Table 13: a kind of method of pest control comprises to the transgenosis pepper and uses the thiophene first extremely, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 14: a kind of method of pest control comprises to transgene cotton and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 15: a kind of method of pest control comprises to transgenic paddy rice and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 16: a kind of method of pest control comprises to transgenic potato and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 17: a kind of method of pest control comprises to transgene tomato and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 18: a kind of method of pest control comprises to the transgenosis cucurbit and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 19: a kind of method of pest control comprises to genetically engineered soybean and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 20: a kind of method of pest control comprises to transgenic corns and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 21: a kind of method of pest control comprises to transgenic wheat and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 22: a kind of method of pest control comprises to the transgenosis banana and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 23: a kind of method of pest control comprises to transgenosis tangerine tree and uses Imidacloprid that wherein the combination of the insect of the active ingredient expressed of genetically modified plants and desire control is corresponding in each combination B.1-B.1l70 of table B any.

Table 24: a kind of method of pest control comprises to transgenosis pomaceous fruits tree and uses Imidacloprid that wherein the combination of the insect of the active ingredient expressed of genetically modified plants and desire control is corresponding in each combination B.1-B.1170 of table B any.

Table 25: a kind of method of pest control comprises to the transgenosis cucurbit and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 26: a kind of method of pest control comprises to the transgenosis pepper and uses Imidacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 27: a kind of method of pest control comprises to transgene cotton and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 28: a kind of method of pest control comprises to transgenic paddy rice and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 29: a kind of method of pest control comprises to transgenic potato and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 30: a kind of method of pest control comprises to the transgenosis rape and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 31: a kind of method of pest control comprises to transgene tomato and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 32: a kind of method of pest control comprises to the transgenosis cucurbit and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 33: a kind of method of pest control comprises to genetically engineered soybean and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 34: a kind of method of pest control comprises to transgenic corns and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 35: a kind of method of pest control comprises to transgenic wheat and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 36: a kind of method of pest control comprises to the transgenosis banana and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 37: a kind of method of pest control comprises to the transgenosis orange tree and uses Ti-435, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 38: a kind of method of pest control comprises to transgenosis pomaceous fruits tree and uses Ti-435 that wherein the combination of the insect of the active ingredient expressed of genetically modified plants and desire control is corresponding in each combination B.1-B.1170 of table B any.

Table 39: a kind of method of pest control comprises to transgene cotton and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 40: a kind of method of pest control comprises to transgenic paddy rice and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 41: a kind of method of pest control comprises to transgenic potato and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 42: a kind of method of pest control comprises to the transgenosis rape and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 43: a kind of method of pest control comprises to transgene tomato and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 44: a kind of method of pest control comprises to the transgenosis cucurbit and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 45: a kind of method of pest control comprises to genetically engineered soybean and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 46: a kind of method of pest control comprises to transgenic corns and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 47: a kind of method of pest control comprises to transgenic wheat and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table 48: a kind of method of pest control comprises to the transgenosis banana and uses thiacloprid, any during wherein B.1-B.1170 the combination of the insect of the active ingredient of genetically modified plants expression and desire control respectively makes up corresponding to table B.

Table C:

Abbreviation:

Acetyl-CoA carboxylase: ACCase

Acetolactate synthase: ALS

Hydroxyphenyl pyruvic acid dioxygenase: HPPD

The inhibition of protein synthesis: IPS

Hormone analogies: HOM

Glutamine synthelase: GS

Proporphyrinogen oxidase: PROTOX

5-enol pyruvoyl-3-phosphoric acid shikimic acid synthase: EPSPS

* * comprises sulfonylurea, imidazolone type, triazolo pyrimidine class, SDM class and N-acyl group sulfamido:

Sulfonylurea such as chlorine sulphur are grand, chlorimuronethyl, ethametsulfuron, metsulfuron-methyl, primisulfuronmethyl, prosulfuron, triasulfuron, cinosulfuron, triflusulfuronmethyl, it is grand to encircle the third oxygen sulphur, bensulfuron-methyl, tribenuron-methyl, ACC 322140, Fluzasulfuron, ethoxysulfuron, Fluzasdulfuron, nicosulfuron, rimsulfuron, thifensulfuronmethyl, pyrazosulfuron, Clopyrasulfuron, NC 330, azimsulfuron, imazosulfuron, lead ethyl xanthate is yellow grand, amidosulfuron, fluorine pyridine sulphur is grand, CGA362622;

Imidazolone type such as miaow oxalic acid, imazaquin, imazamethypyr, imazethapyr, imidazoles nicotinic acid and imazamox;

Triazolo pyrimidine class such as DE 511, Flumetsulam and Chloransulam;

SDM class such as pyrithiobac-sodium, oxime pyridine grass, two careless ether and phonetic benzene grass oxime.

+++tolerance diclofop-methyl, efficient fluazifop, haloxyfop-methyl, the spirit of smart fluorine pyrrole second standing grain, Quizalotop-ethyl, alkynes oxalic acid, fenoxapropPethyl, Tepraloxydim, alloxydimsodium, sethoxydim, cycloxydim, cyclohexene humulone, tralkoxydim, Butoxydim, quinone oximes grass, Clefoxydim, clethodim.

﹠﹠﹠ acetyl chlorobenzene amine such as alachlor, Acetochlor, dimethenamid.

///Protox inhibitor, for example diphenyl ether such as acifluorfen, aclonifen, bifenox, Mo 9 granular, Ethoxyfen, fluoroglycofen-ethyl, fomesafen, lactofen, Oxyfluorfen; Imidodicarbonic diamide such as azoles pyridine alkynes grass, fluorine ketazolam grass, Cinidon-ethyl, Flumiclorac pentyl, flumioxazin, reach careless fluorine, the alkynes third oxazole grass, oxadiazon, penta oxazole grass, sulfentrazone, imidodicarbonic diamide and other is as Flumipropyn, Flupropacil, nipyralofen and thiadiazoles amine; Also have Fluazolate and fluorine carfentrazone.Biology embodiment

Table 49: a kind of representative biological method of preventing and treating the Adoxophyes spp genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 50: a kind of representative biological method of preventing and treating ground tiger genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 51: a kind of method of preventing and treating cotton leaf noctuid; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 52: a kind of method of preventing and treating the soybean noctuid; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 53: a kind of representative biological method of preventing and treating straw borer spp; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 54: a kind of method of preventing and treating grape codling moth (Clysia ambiguella); comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 55: a kind of representative biological method of preventing and treating the Cnaphalocrocis spp genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 56: a kind of method of preventing and treating general non-fine hair snout moth's larva; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 57: a kind of representative biological method of preventing and treating the steinernema genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 58: a kind of method of preventing and treating the Sudan cotton bollworm; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 59: a kind of representative biological method of preventing and treating golden steel brill genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 60: a kind of representative biological method of preventing and treating the meal moth genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 61: a kind of representative biological method of preventing and treating Heliothis; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 62: a kind of method of preventing and treating Hellula undalis; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 63: a kind of method of preventing and treating tomato stem gelechiid; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 64: a kind of method of preventing and treating pear leaf blister moth; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 65: a kind of representative biological method of preventing and treating thin moth genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 66: a kind of method of preventing and treating Lobesia botrana; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 67: a kind of method of preventing and treating European corn borer; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 68: a kind of representative biological method of preventing and treating brown epiblema; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 69: a kind of method of preventing and treating Pectinophora gossypiella; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 70: a kind of method of preventing and treating citrus leaf lyonetid; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 71: a kind of representative biological method of preventing and treating Pier; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 72: a kind of method of preventing and treating diamond-back moth; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 73: a kind of representative biological method of preventing and treating white standing grain snout moth's larva genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 74: a kind of representative biological method of preventing and treating moth stem Noctua; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 75: a kind of representative biological method of preventing and treating long palpus volume moth genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 76: a kind of representative biological method of preventing and treating Spodoptera; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 77: a kind of representative biological method of preventing and treating volume moth genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 78: a kind of method of preventing and treating the amyloid plaque noctuid; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 79: a kind of representative biological method of preventing and treating Agriotes spp; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 80: a kind of method of preventing and treating Mexico cotton boll elephant; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 81: a kind of representative biological method of preventing and treating Curculio; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 82: a kind of chrysomelid method of band spot cucumber of preventing and treating; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 83: a kind of representative biological method of preventing and treating the colorado potato beetles genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 84: a kind ofly prevent and treat the representative biological method that rice water resembles genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 85: a kind ofly prevent and treat the representative biological method that the ear beak resembles genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 86: a kind of representative biological method of preventing and treating the wolly whitefly genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 87: a kind of representative biological method of preventing and treating Aleyrodes; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 88: a kind of representative biological method of preventing and treating kidney Aspidiotus genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 89: a kind of representative biological method of preventing and treating Aphidiadae; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 90: a kind of representative biological method of preventing and treating Aphis; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 91: a kind of method of preventing and treating sweet potato whitefly; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 92: a kind of representative biological method of preventing and treating green jassids genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 93: a kind of representative biological method of preventing and treating tumor aphid genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 94: a kind of representative biological method of preventing and treating the rice leafhopper genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 95: a kind of representative biological method of preventing and treating the brown planthopper genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 96: a kind of representative biological method of preventing and treating the mealybug genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 97: a kind of representative biological method of preventing and treating Psylla spp; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 98: a kind of representative biological method of preventing and treating large bamboo hat with a conical crown and broad brim Aspidiotus genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 99: a kind of representative biological method of preventing and treating the y-bend Aphis; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 100: a kind of representative biological method of preventing and treating knot wing Aleyrodes; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 101: a kind of representative biological method of preventing and treating Lyriomyza; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 102: a kind of representative biological method of preventing and treating the wheat stem chloropid fly genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 103: a kind of representative biological method of preventing and treating careless Hylemyia; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 104: a kind of representative biological method of preventing and treating the flower thrips genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 105: a kind of representative biological method of preventing and treating Thrips; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 106: a kind of method of preventing and treating hard thrips; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 107: a kind of representative biological method of preventing and treating the aceria genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 108: a kind of representative biological method of preventing and treating the thorn Eriophyes; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 109: a kind of representative biological method of preventing and treating short whisker Acarapis; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 110: a kind of representative biological method of preventing and treating the Panonychus citri genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 111: a kind of representative biological method of preventing and treating wrinkle leaf Aculus genus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 112: a kind of representative biological method of preventing and treating Tetranychus; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 113: a kind of representative biological method of preventing and treating Heterodera; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 114: a kind of representative biological method of preventing and treating Meloidogyne; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 115: a kind of method of preventing and treating tomato moth; comprise that using the thiophene first to the genetically modified crops of herbicide-tolerant kills, wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 116: a kind of representative biological method of preventing and treating the Adoxophyes spp genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 117: a kind of representative biological method of preventing and treating ground tiger genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect injury of the active ingredient expressed of genetically modified plants and protection is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 118: a kind of method of preventing and treating cotton leaf noctuid; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect injury of the active ingredient expressed of genetically modified plants and protection is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 119: a kind of method of preventing and treating the soybean noctuid; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect injury of the active ingredient expressed of genetically modified plants and protection is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 120: a kind of representative biological method of preventing and treating straw borer spp; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 121: a kind of method of preventing and treating grape codling moth (Clysia ambiguella); comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 122: a kind of representative biological method of preventing and treating the Cnaphalocrocis spp genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 123: a kind of method of preventing and treating general non-fine hair snout moth's larva; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 124: a kind of representative biological method of preventing and treating the steinernema genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 125: the method for a kind of control the Sudan cotton bollworm (); comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 126: a kind of representative biological method of preventing and treating golden steel brill genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 127: a kind of representative biological method of preventing and treating the meal moth genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 128: a kind of representative biological method of preventing and treating Heliothis; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 129: a kind of method of preventing and treating Hellula undalis; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 130: a kind of method of preventing and treating tomato stem gelechiid; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 131: a kind of method of preventing and treating pear leaf blister moth; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 132: a kind of representative biological method of preventing and treating thin moth genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 133: a kind of method of preventing and treating Lobesia botrana; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 134: a kind of method of preventing and treating European corn borer; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 135: a kind of representative biological method of preventing and treating brown epiblema; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 136: a kind of method of preventing and treating Pectinophora gossypiella; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 137: a kind of method of preventing and treating citrus leaf lyonetid; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 138: a kind of representative biological method of preventing and treating Pier; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 139: a kind of method of preventing and treating diamond-back moth; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 140: a kind of representative biological method of preventing and treating white standing grain snout moth's larva genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 141: a kind of representative biological method of preventing and treating moth stem Noctua; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 142: a kind of representative biological method of preventing and treating long palpus volume moth genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 143: a kind of representative biological method of preventing and treating Spodoptera; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 144: a kind of representative biological method of preventing and treating volume moth genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 145: a kind of method of preventing and treating the amyloid plaque noctuid; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 146: a kind of representative biological method of preventing and treating Agriotes spp; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 147: a kind of method of preventing and treating Mexico cotton boll elephant; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 148: a kind of representative biological method of preventing and treating Curculio; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 149: a kind of chrysomelid method of band spot cucumber of preventing and treating; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 150: a kind of representative biological method of preventing and treating the colorado potato beetles genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 151: a kind ofly prevent and treat the representative biological method that rice water resembles genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 152: a kind ofly prevent and treat the representative biological method that the ear beak resembles genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 153: a kind of representative biological method of preventing and treating the wolly whitefly genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 154: a kind of representative biological method of preventing and treating Aleyrodes; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 155: a kind of representative biological method of preventing and treating kidney Aspidiotus genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 156: a kind of representative biological method of preventing and treating Aphidiadae; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 157: a kind of representative biological method of preventing and treating Aphis; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 158: a kind of method of preventing and treating sweet potato whitefly; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 159: a kind of representative biological method of preventing and treating green jassids genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 160: a kind of representative biological method of preventing and treating tumor aphid genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 161: a kind of representative biological method of preventing and treating the rice leafhopper genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 162: a kind of representative biological method of preventing and treating the brown planthopper genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 163: a kind of representative biological method of preventing and treating the mealybug genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 164: a kind of representative biological method of preventing and treating Psylla spp; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 165: a kind of representative biological method of preventing and treating large bamboo hat with a conical crown and broad brim Aspidiotus genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 166: a kind of representative biological method of preventing and treating the y-bend Aphis; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 167: a kind of representative biological method of preventing and treating knot wing Aleyrodes; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 168: a kind of representative biological method of preventing and treating Lyriomyza; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 169: a kind of representative biological method of preventing and treating the wheat stem chloropid fly genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 170: a kind of representative biological method of preventing and treating careless Hylemyia; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 171: a kind of representative biological method of preventing and treating the flower thrips genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 172: a kind of representative biological method of preventing and treating Thrips; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 173: a kind of method of preventing and treating hard thrips; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 174: a kind of representative biological method of preventing and treating the aceria genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 175: a kind of representative biological method of preventing and treating the thorn Eriophyes; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 176: a kind of representative biological method of preventing and treating short whisker Acarapis; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 177: a kind of representative biological method of preventing and treating the Panonychus citri genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 178: a kind of representative biological method of preventing and treating wrinkle leaf Aculus genus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 179: a kind of representative biological method of preventing and treating Tetranychus; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 180: a kind of representative biological method of preventing and treating Heterodera; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 181: a kind of representative biological method of preventing and treating Meloidogyne; comprise to the genetically modified crops of herbicide-tolerant and use Imidacloprid that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 182: a kind of representative biological method of preventing and treating the Adoxophyes spp genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 183: a kind of representative biological method of preventing and treating ground tiger genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect injury of the active ingredient expressed of genetically modified plants and protection is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 184: a kind of method of preventing and treating cotton leaf noctuid; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect injury of the active ingredient expressed of genetically modified plants and protection is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 185: a kind of method of preventing and treating the soybean noctuid; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect injury of the active ingredient expressed of genetically modified plants and protection is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 186: a kind of representative biological method of preventing and treating straw borer spp; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 187: a kind of method of preventing and treating grape codling moth (Clysia ambiguella); comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 188: a kind of method of preventing and treating general non-fine hair snout moth's larva; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 189: a kind of representative biological method of preventing and treating the steinernema genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 190: a kind of method of preventing and treating the Sudan cotton bollworm; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 191: a kind of representative biological method of preventing and treating golden steel brill genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 192: a kind of representative biological method of preventing and treating the meal moth genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 193: a kind of representative biological method of preventing and treating Heliothis; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 194: a kind of method of preventing and treating Hellula undalis; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 195: a kind of method of preventing and treating tomato stem gelechiid; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 196: a kind of method of preventing and treating pear leaf blister moth; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 197: a kind of representative biological method of preventing and treating thin moth genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 198: a kind of method of preventing and treating Lobesia botrana; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 199: a kind of method of preventing and treating European corn borer; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 200: a kind of representative biological method of preventing and treating brown epiblema; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 201: a kind of method of preventing and treating Pectinophora gossypiella; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 202: a kind of method of preventing and treating citrus leaf lyonetid; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 203: a kind of representative biological method of preventing and treating Pier; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 204: a kind of method of preventing and treating diamond-back moth; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 205: a kind of representative biological method of preventing and treating white standing grain snout moth's larva genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 206: a kind of representative biological method of preventing and treating moth stem Noctua; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 207: a kind of representative biological method of preventing and treating long palpus volume moth genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 208: a kind of representative biological method of preventing and treating Spodoptera; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 209: a kind of representative biological method of preventing and treating volume moth genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 210: a kind of method of preventing and treating the amyloid plaque noctuid; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 211: a kind of representative biological method of preventing and treating Agriotes spp; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 212: a kind of method of preventing and treating Mexico cotton boll elephant; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 213: a kind of representative biological method of preventing and treating Curculio; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 214: a kind of chrysomelid method of band spot cucumber of preventing and treating; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 215: a kind of representative biological method of preventing and treating the colorado potato beetles genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 216: a kind ofly prevent and treat the representative biological method that rice water resembles genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 217: a kind ofly prevent and treat the representative biological method that the ear beak resembles genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 218: a kind of representative biological method of preventing and treating the wolly whitefly genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 219: a kind of representative biological method of preventing and treating Aleyrodes; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 220: a kind of representative biological method of preventing and treating kidney Aspidiotus genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 221: a kind of representative biological method of preventing and treating Aphidiadae; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 222: a kind of representative biological method of preventing and treating Aphis; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 223: a kind of method of preventing and treating sweet potato whitefly; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 224: a kind of representative biological method of preventing and treating green jassids genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 225: a kind of representative biological method of preventing and treating tumor aphid genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 226: a kind of representative biological method of preventing and treating the rice leafhopper genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 227: a kind of representative biological method of preventing and treating the brown planthopper genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 228: a kind of representative biological method of preventing and treating the mealybug genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 229: a kind of representative biological method of preventing and treating Psylla spp; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 230: a kind of representative biological method of preventing and treating large bamboo hat with a conical crown and broad brim Aspidiotus genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 231: a kind of representative biological method of preventing and treating the y-bend Aphis; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 232: a kind of representative biological method of preventing and treating knot wing Aleyrodes; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 233: a kind of representative biological method of preventing and treating Lyriomyza; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 234: a kind of representative biological method of preventing and treating the wheat stem chloropid fly genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 235: a kind of representative biological method of preventing and treating careless Hylemyia; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 236: a kind of representative biological method of preventing and treating the flower thrips genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 237: a kind of representative biological method of preventing and treating Thrips; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 238: a kind of method of preventing and treating hard thrips; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 239: a kind of representative biological method of preventing and treating the aceria genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 240: a kind of representative biological method of preventing and treating the thorn Eriophyes; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 241: a kind of representative biological method of preventing and treating short whisker Acarapis; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 242: a kind of representative biological method of preventing and treating the Panonychus citri genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 243: a kind of representative biological method of preventing and treating wrinkle leaf Aculus genus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 244: a kind of representative biological method of preventing and treating Tetranychus; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 245: a kind of representative biological method of preventing and treating Heterodera; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 246: a kind of representative biological method of preventing and treating Meloidogyne; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.

Table 247: a kind of method of preventing and treating tomato moth; comprise to the genetically modified crops of herbicide-tolerant and use Ti-435 that wherein its combination of crop that is not subjected to this insect infringement of the active ingredient and protection expressed of genetically modified plants is corresponding to arbitrary row of row C.1-C.108 among the table C.Embodiment B 1: the effect that Mexico's cotton boll is resembled adult, Spodoptera littoralis or tobacco budworm

Respectively with contain 100,50,10,5, the aqueous emulsion Spray Mixing thing of 1ppm Imidacloprid sprays the transgene cotton seedling of expressing delta-endotoxin Cry III A.After the coating drying to be painted, on cotton plants, add 10 Mexico's cotton bolls respectively and resemble adult, 10 Spodoptera littoralis larvas or 10 tobacco budworm larvas, and cotton is moved in the plastic containers.Assess after 3-10 days.By comparing the beetle quantity dead on transgenic cotton and the non-transgenic cotton and the damage of ingesting, determine the minimizing percentage of population or the reduction percentage (% effect) of the damage of ingesting, in all cases, described non-transgenic cotton concentration is 100,50,10,5 with containing respectively, the Imidacloprid of 1ppm and the emulsion Spray Mixing thing of conventional Cry III A-toxin are handled.

In this test, the control of test insect on the genetically modified plants is better than contrast on the non-transgenic plant.Embodiment B 2: the effect that Mexico's cotton boll is resembled adult, Spodoptera littoralis or tobacco budworm

Spray the transgene cotton seedling of expressing delta-endotoxin Cry III A with the aqueous emulsion Spray Mixing thing that contains 100,50,10,5, the thiophene first of 1ppm is killed respectively.After the coating drying to be painted, add on cotton plants respectively that 10 Mexico's cotton bolls resemble, 10 Spodoptera littoralis larvas or 10 tobacco budworm larvas, and cotton moved in the plastic containers.Assess after 3-10 days.By comparing the beetle quantity dead on transgene cotton and the non-transgenic cotton and the damage of ingesting, determine the minimizing percentage of population or the reduction percentage (% effect) of the damage of ingesting, in all cases, described non-transgenic cotton concentration is 100,50,10,5, the thiophene first of 1ppm is killed and the emulsion Spray Mixing thing of conventional Cry III A-toxin is handled with containing respectively.

In this test, be excellent to the control of testing insect on the genetically modified plants, and the control deficiency of test insect on the non-transgenic plant.Embodiment B 3: the effect that Mexico's cotton boll is resembled adult, Spodoptera littoralis or tobacco budworm

Respectively with contain 100,50,10,5, the aqueous emulsion Spray Mixing thing of the Ti-435 of 1ppm sprays the transgene cotton seedling of expressing delta-endotoxin Cry III A.After the coating drying to be painted, add on cotton plants respectively that 10 Mexico's cotton bolls resemble, 10 Spodoptera littoralis larvas or 10 tobacco budworm larvas, and cotton moved in the plastic containers.Assess after 3-10 days.By comparing the beetle quantity dead on transgene cotton and the non-transgenic cotton and the damage of ingesting, determine the minimizing percentage of population or the reduction percentage (% effect) of the damage of ingesting, there is something special down, and concentration is 100,50,10,5 to described non-transgenic cotton with containing respectively, the Ti-435 of 1ppm and the emulsion Spray Mixing thing of conventional Cry III A-toxin are handled.

In this test, be excellent to the control of testing insect on the genetically modified plants, and the control deficiency of test insect on the non-transgenic plant.Embodiment B 4: the effect that Mexico's cotton boll is resembled adult, Spodoptera littoralis or tobacco budworm

Respectively with contain 100,50,10,5, the aqueous emulsion Spray Mixing thing of the Ti-435 of 1ppm sprays the transgene cotton seedling of expressing delta-endotoxin Cry I a (c).After the coating drying to be painted, add on cotton plants respectively that 10 Mexico's cotton bolls resemble, 10 Spodoptera littoralis larvas or 10 tobacco budworm larvas, and cotton moved in the plastic containers.Assess after 3-10 days.By comparing the beetle quantity dead on transgene cotton and the non-transgenic cotton and the damage of ingesting, determine the minimizing percentage of population or the reduction percentage (% effect) of the damage of ingesting, in all cases, described non-transgenic cotton concentration is 100,50,10,5 with containing respectively, the Ti-435 of 1ppm and the emulsion Spray Mixing thing of conventional Cry III A-toxin are handled.

In this test, be excellent to the control of testing insect on the genetically modified plants, and the control deficiency of test insect on the non-transgenic plant.Embodiment B 5: the effect that Mexico's cotton boll is resembled adult, Spodoptera littoralis or tobacco budworm

Spray the transgene cotton seedling of expressing delta-endotoxin Cry I a (c) with the aqueous emulsion Spray Mixing thing that contains 100,50,10,5, the thiophene first of 1ppm is killed respectively.After the coating drying to be painted, add on cotton plants respectively that 10 Mexico's cotton bolls resemble, 10 Spodoptera littoralis larvas or 10 tobacco budworm larvas, and cotton moved in the plastic containers.Assess after 3-10 days.By comparing the beetle quantity dead on transgene cotton and the non-transgenic cotton and the damage of ingesting, determine the minimizing percentage of population or the reduction percentage (% effect) of the damage of ingesting, in all cases, described non-transgenic cotton concentration is 100,50,10,5, the thiophene first of 1ppm is killed and the emulsion Spray Mixing thing of conventional Cry III A-toxin is handled with containing respectively.

In this test, be excellent to the control of testing insect on the genetically modified plants, and the control deficiency of test insect on the non-transgenic plant.Embodiment B 6: the effect that Mexico's cotton boll is resembled adult, Spodoptera littoralis or tobacco budworm

Respectively with contain 100,50,10,5, the aqueous emulsion Spray Mixing thing of the Imidacloprid of 1ppm sprays the transgene cotton seedling of expressing delta-endotoxin Cry I a (c).After the coating drying to be painted, add on cotton plants respectively that 10 Mexico's cotton bolls resemble, 10 Spodoptera littoralis larvas or 10 tobacco budworm larvas, and cotton moved in the plastic containers.Assess after 3-10 days.By comparing the beetle quantity dead on transgene cotton and the non-transgenic cotton and the damage of ingesting, determine the minimizing percentage of population or the reduction percentage (% effect) of the damage of ingesting, in all cases, described non-transgenic cotton concentration is 100,50,10,5 with containing respectively, the Imidacloprid of 1ppm and the emulsion Spray Mixing thing of conventional Cry III A-toxin are handled.

In this test, be excellent to the control of testing insect on the genetically modified plants, and the control deficiency of test insect on the non-transgenic plant.Embodiment B 7: to the effect of the kind of the kind of European corn borer, Spodoptera or Heliothis

Sample ground (a) maize planting cv.KnockOut ^Adjacent onesize sample ground (b) plantation ordinary maize, two sample ground all shows the kind that is subjected to European corn borer, Spodoptera or the infringement naturally of Heliothis, with contain 200,100,50,10,5, the aqueous emulsion Spray Mixing thing of the Ti-435 of 1ppm sprays.Afterwards immediately with contain 200,100,50,10,5, the KnockOut of 1ppm ^Expressed endotoxic emulsion Spray Mixing thing treatment samples ground (b).Assess after 6 days.Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).

On the plant of sample ground (a), observe the kind of European corn borer, Spodoptera or the level of preventing and treating of Heliothis are improved, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 8: to the effect of the kind of the kind of European corn borer, Spodoptera or Heliothis

Sample ground (a) maize planting cv.KnockOut ^Adjacent onesize sample ground (b) plantation ordinary maize, two sample ground all shows the kind that is subjected to European corn borer, Spodoptera or the infringement naturally of Heliothis, sprays with the aqueous emulsion Spray Mixing thing that contains 200,100,50,10,5, the thiophene first of 1ppm is killed respectively.Afterwards immediately with contain 200,100,50, the KnockOut of 1O, 5,1ppm ^Expressed endotoxic emulsion Spray Mixing thing treatment samples ground (b).Assess after 6 days.Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).

On the plant of sample ground (a), observe the kind of European corn borer, Spodoptera or the level of preventing and treating of Heliothis are improved, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 9: to the effect of the kind of the kind of European corn borer, Spodoptera or Heliothis

Sample ground (a) maize planting cv.KnockOut ^Adjacent onesize sample ground (b) plantation ordinary maize, two sample ground all shows the kind that is subjected to European corn borer, Spodoptera or the infringement naturally of Heliothis, respectively with contain 200,100,50,10,5, the aqueous emulsion Spray Mixing thing of the Imidacloprid of 1ppm sprays.Afterwards immediately with contain 200,100,50,10,5, the KnockOut of 1ppm ^Expressed endotoxic emulsion Spray Mixing thing treatment samples ground (b).Assess after 6 days.Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).

On the plant of sample ground (a), observe the kind of European corn borer, Spodoptera or the level of preventing and treating of Heliothis are improved, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 10: to the chrysomelid effect of band spot cucumber

Sample ground (a) maize planting cv.KnockOut ^, adjacent onesize sample ground (b) plantation ordinary maize is with containing the Spray Mixing thing aqueous emulsion sprinkling that 400ppm thiophene first is killed.Afterwards immediately with the KnockOut that contains 400ppm ^Expressed endotoxic emulsion Spray Mixing thing treatment samples ground (b).After the coating drying to be painted, on seedling, add 10 chrysomelid larvas of band spot cucumber, and seedling is transferred in the plastic containers in second stage.This test of assessment after 6 days.Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).

On the plant of sample ground (a), observe the chrysomelid level of preventing and treating of band spot cucumber is improved, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 11: to the effect of cotten aphid

Make the cotton seedling of expression delta-endotoxin Cry III a in the sample ground (a) and the conventional cotton seedling in sample ground (b) infect cotten aphid, subsequently with containing the Spray Mixing thing sprinkling that 400ppm thiophene first is killed.Afterwards immediately with the emulsion Spray Mixing thing treatment samples ground (b) that contains 400ppm delta-endotoxin Cry III a.Then with sample ground (a) and seedling (b) at 20 ℃ of following incubations.This test of assessment after 3-6 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).The level of observing on the plant of sample ground (a) cotten aphid of preventing and treating improves, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 12: to the effect of west flower thrips (Frankliniella occidentalis)

Make the cotton seedling of expression delta-endotoxin Cry III a in the sample ground (a) and the conventional cotton seedling in sample ground (b) infect the west flower thrips, subsequently with containing the Spray Mixing thing sprinkling that 400ppm thiophene first is killed.Afterwards immediately with the emulsion Spray Mixing thing treatment samples ground (b) that contains 400ppm delta-endotoxin Cry III a.Then with sample ground (a) and seedling (b) at 20 ℃ of following incubations.This test of assessment after 3-6 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).The level of observing on the plant of sample ground (a) the west flower thrips of preventing and treating improves, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 13: to the effect of cotten aphid

Make the cotton seedling of expression delta-endotoxin Cry I A (c) in the sample ground (a) and the conventional cotton seedling in sample ground (b) infect cotten aphid, subsequently with containing the Spray Mixing thing sprinkling that 400ppm thiophene first is killed.Afterwards immediately with the emulsion Spray Mixing thing treatment samples ground (b) that contains 400ppm delta-endotoxin Cry III a.Then with sample ground (a) and seedling (b) at 20 ℃ of following incubations.This test of assessment after 3-6 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).The level of observing on the plant of sample ground (a) cotten aphid of preventing and treating improves, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 14: to the effect of west flower thrips

Make the cotton seedling of expression delta-endotoxin Cry I a (c) in the sample ground (a) and the conventional cotton seedling in sample ground (b) infect the west flower thrips, subsequently with containing the Spray Mixing thing sprinkling that 400ppm thiophene first is killed.Afterwards immediately with the emulsion Spray Mixing thing treatment samples ground (b) that contains 400ppm delta-endotoxin Cry I a (c).Then with sample ground (a) and seedling (b) at 20 ℃ of following incubations.This test of assessment after 3-6 days.Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).The level of observing on the plant of sample ground (a) the west flower thrips of preventing and treating improves, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 15: to the effect of rice leafhopper

Spray the paddy rice of expression delta-endotoxin Cry I A (b) in the sample ground (a) and the conventional rice in sample ground (b) with containing the Spray Mixing thing that 400ppm thiophene first kills.Afterwards immediately with the emulsion Spray Mixing thing treatment samples ground (b) that contains 400ppm delta-endotoxin Cry I A (b).After the coating drying to be painted, make the rice leafhopper of plant infection second and phase III.Then with sample ground (a) and seedling (b) at 20 ℃ of following incubations.This test of assessment after 21 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).The level of observing on the plant of sample ground (a) rice leafhopper of preventing and treating improves, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 16: to the effect (interior suction) of rice leafhopper

Paddy rice of delta-endotoxin Cry I a (b) is expressed in plantation in the jar (A), jar (B) middle plantation conventional rice.Jar (A) placed contains the aqueous emulsion that 400ppm thiophene first is killed, and jar (B) place contain that 400ppm thiophene first is killed and 400ppm delta-endotoxin Cry I (b) jar.Subsequently with second and the rice leafhopper larva of phase III infect these plants.This test of assessment after 6 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing jar (A) and jar (B).The level of observing on the plant of jar (A) rice leafhopper of preventing and treating improves, and jar (B) shows 60% the level of preventing and treating that is no more than.Embodiment B 17: to the effect of brown planthopper

Make the paddy rice and the conventional rice in sample ground (b) of expressing delta-endotoxin Cry I A (b) in the sample ground (a) infect brown planthopper, subsequently with containing the Spray Mixing thing sprinkling that 400ppm thiophene first is killed.Afterwards immediately with the emulsion Spray Mixing thing treatment samples ground (b) that contains 400ppm delta-endotoxin Cry I A (b).Then with sample ground (a) and seedling (b) at 20 ℃ of following incubations.This test of assessment after 21 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).The level of observing on the plant of sample ground (a) brown planthopper of preventing and treating improves, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 18: to the effect (interior suction) of brown planthopper

Paddy rice of delta-endotoxin Cry I A (b) is expressed in plantation in the jar (A), jar (B) middle plantation conventional rice.Jar (A) placed contains the aqueous emulsion that 400ppm thiophene first is killed, and jar (B) place contain that 400ppm thiophene first is killed and 400ppm delta-endotoxin Cry I A (b) jar.Subsequently with second and the brown planthopper larva of phase III infect these plants.This test of assessment after 6 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing jar (A) and jar (B).The level of observing on the plant of jar (A) brown planthopper of preventing and treating improves, and jar (B) shows 60% the level of preventing and treating that is no more than.Embodiment B 19: to the effect of rice leafhopper

Spray the paddy rice that makes expression delta-endotoxin Cry I A (c) in the sample ground (a) and the conventional rice in sample ground (b) with containing Spray Mixing thing that 400ppm thiophene first kills.Afterwards immediately with the emulsion Spray Mixing thing treatment samples ground (b) that contains 400ppm delta-endotoxin Cry I A (c).After the coating drying to be painted, with second and the rice leafhopper of phase III infect these plants.Then with sample ground (a) and seedling (b) at 20 ℃ of following incubations.This test of assessment after 21 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).The level of observing on the plant of sample ground (a) rice leafhopper of preventing and treating improves, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 20: to the effect (interior suction) of rice leafhopper

Paddy rice of delta-endotoxin Cry I a (c) is expressed in plantation in the jar (A), jar (B) middle plantation conventional rice.Jar (A) placed contains the aqueous emulsion that 400ppm thiophene first is killed, and jar (B) place contain that 400ppm thiophene first is killed and 400ppm delta-endotoxin Cry I (c) jar.Subsequently with second and the rice leafhopper larva of phase III infect these plants.This test of assessment after 6 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing jar (A) and jar (B).The level of observing on the plant of jar (A) rice leafhopper of preventing and treating improves, and jar (B) shows 60% the level of preventing and treating that is no more than.Embodiment B 21: to the effect of brown planthopper

Make the paddy rice and the conventional rice in sample ground (b) of expressing delta-endotoxin Cry I A (c) in the sample ground (a) infect brown planthopper, subsequently with containing the Spray Mixing thing sprinkling that 400ppm thiophene first is killed.Afterwards immediately with the emulsion Spray Mixing thing treatment samples ground (b) that contains 400ppm delta-endotoxin Cry I A (c).Then with sample ground (a) and seedling (b) at 20 ℃ of following incubations.This test of assessment after 21 days.

Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing sample ground (a) and sample ground (b).The level of observing on the plant of sample ground (a) brown planthopper of preventing and treating improves, and sample ground (b) shows 60% the level of preventing and treating that is no more than.Embodiment B 22: to the effect (interior suction) of brown planthopper

Paddy rice of delta-endotoxin CryIA (c) is expressed in plantation in the jar (A), jar (B) middle plantation conventional rice.Jar (A) placed contains the aqueous emulsion that 400ppm thiophene first is killed, and jar (B) place contain that 400ppm thiophene first is killed and 400ppm delta-endotoxin CryIA (c) jar.Subsequently with second and the brown planthopper larva of phase III infect these plants.This test of assessment after 6 days.Determine the minimizing percentage (% effect) of population by number of pest dead on the plant of comparing jar (A) and jar (B).The level of observing on the plant of jar (A) brown planthopper of preventing and treating improves, and jar (B) shows 60% the level of preventing and treating that is no more than.

Claims

1. prevent and treat the method for insect in the crop of transgenosis useful plants, it is characterized in that using a kind of Pesticidal combination to insect or its environment, said composition comprise the nitro imino group of salt form useful on free form or the agrochemistry-or nitro guanidine radicals-compound as active ingredient and at least a auxiliary agent.

2. according to the method for claim 1, it is characterized in that using the thiophene first to kill.

3. according to the method for claim 1, it is characterized in that using Imidacloprid.

4. according to the method for claim 1, it is characterized in that genetically modified plants obtain handling.

5. according to each method among the claim 1-4, the genetically modified crops that it is characterized in that described useful plants are corns.

6. according to each method among the claim 1-4, the genetically modified crops that it is characterized in that described useful plants are soybean.

7. according to the method for claim 4, it is characterized in that the propagating materials of transgenosis useful plants obtains handling.