CN1290554A - Extracting method for effective active matter of lucid ganoderma spore - Google Patents
Extracting method for effective active matter of lucid ganoderma spore Download PDFInfo
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- CN1290554A CN1290554A CN 00117466 CN00117466A CN1290554A CN 1290554 A CN1290554 A CN 1290554A CN 00117466 CN00117466 CN 00117466 CN 00117466 A CN00117466 A CN 00117466A CN 1290554 A CN1290554 A CN 1290554A
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- ganoderma spore
- extraction
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- breaking
- sporoderm
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- 241000222336 Ganoderma Species 0.000 title claims abstract description 84
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000012530 fluid Substances 0.000 claims abstract description 33
- 108010059892 Cellulase Proteins 0.000 claims abstract description 7
- 102000012286 Chitinases Human genes 0.000 claims abstract description 7
- 108010022172 Chitinases Proteins 0.000 claims abstract description 7
- 229940106157 cellulase Drugs 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims description 59
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 25
- 230000001413 cellular effect Effects 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 9
- 239000003814 drug Substances 0.000 abstract description 2
- 239000013543 active substance Substances 0.000 description 14
- 238000000703 high-speed centrifugation Methods 0.000 description 13
- 238000012360 testing method Methods 0.000 description 12
- 229960004756 ethanol Drugs 0.000 description 11
- 230000003647 oxidation Effects 0.000 description 11
- 238000007254 oxidation reaction Methods 0.000 description 11
- 239000003205 fragrance Substances 0.000 description 10
- 239000004519 grease Substances 0.000 description 10
- 238000001556 precipitation Methods 0.000 description 10
- 239000012153 distilled water Substances 0.000 description 7
- 239000002904 solvent Substances 0.000 description 7
- 230000007071 enzymatic hydrolysis Effects 0.000 description 5
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 5
- 240000008397 Ganoderma lucidum Species 0.000 description 4
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000000108 ultra-filtration Methods 0.000 description 4
- 239000002775 capsule Substances 0.000 description 3
- 108010017842 Telomerase Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 102000011759 adducin Human genes 0.000 description 1
- 108010076723 adducin Proteins 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The present invention relates to the wall breaking and supercritical CO2 fluid extracting process of obtaining effective active matter of glossy ganoderma spore. Ripe and plump glossy ganoderma spore is wall broken through medicine superfine breaking process and before breaking, chitinase or cellulase may be added to enzymolyze and soften spore wall. Wall broken glossy ganoderma spore is extracted with supercritical CO2 fluid, with the extracted oily effective active matter accounting for 37-37.6 % of the total weight of extracted glossy ganoderma spore.
Description
The present invention relates to a kind of with Ganoderma spore through breaking cellular wall and supercritical CO
2Fluid (SFE-CO
2) abstraction technique obtains the method for effective active matter of lucid ganoderma spore.
Ganoderma is a Basidiomycetes Polyporaceae Ganoderma fungus.Ganoderma spore is the atomic thin avette spore of brown (6-7 * 10-12 μ m) that ejects from the cap bottom in the Ganoderma period of maturation.It is documented that Ganoderma spore has the whole hereditary active substance of Ganoderma.Because Ganoderma spore sporoderm compact structure and tough and tensile unusually, conventional extracting method is difficult to the effective active matter of extracting to Ganoderma spore, causes abundant being of the medical value of crucial importance sebaceous active substance of having of Ganoderma spore wall capsule intensive amount not to be widely used.Have document to point out, the drug effect of Ganoderma spore and value are relevant with the wall breaking technology or the sporoderm-broken rate of Ganoderma spore, the product that sporoderm-broken rate is high more, easy more being absorbed by the body.Have research to point out, to the test of Ganoderma spore active component, polysaccharide detects and is not suitable for the Ganoderma spore sample.Be subjected to extensive concern at Ganoderma spore physiologically active ingredient and anticancer mechanism and drug development thereof.
The method that the purpose of this invention is to provide a kind of high efficiency extraction effective active matter of lucid ganoderma spore is so that Ganoderma spore obtains fully, effectively utilization.
The present invention finds, in time gathers the Ganoderma spore of cultivating with log, since the freshness height, the nutrient supply abundance, the spore mature and plump adopts the conventional method breaking cellular wall, and sporoderm-broken rate is high.Experiment finds that the Ganoderma spore of breaking cellular wall can disengage the sebaceous active substance that is that is stored in the Ganoderma spore wall capsule, through supercritical CO
2Fluid extraction can obtain the sebaceous effective active matter that is up to 37.6%; And without the Ganoderma spore of breaking cellular wall, equal conditions extraction yield less than 3.5%.Zoopery and clinical test results show that effective active matter of lucid ganoderma spore has obvious inhibitory action to hepatocarcinoma and liver cancer tissue telomerase activation; The person has obvious curative effects to the HBV viral infection.As seen the effective active matter of lucid ganoderma spore that disengages of breaking cellular wall is obvious to function of tumor inhibition.
Method of the present invention is through breaking cellular wall and supercritical CO with Ganoderma spore
2Fluid extraction technology obtains effective active matter of lucid ganoderma spore, promptly utilize near critical point, the minor variations of system temperature and pressure can cause the sudden change of dissolubility generation several magnitude and the enhanced characteristic of penetration to realize separating with sporoderm being sebaceous active substance in the Ganoderma spore wall capsule, in supercritical fluid extraction equipment, finish extraction simultaneously and distill the operation of two steps, the separation efficiency height, the effective active matter of lucid ganoderma spore of extraction can keep original physiologically active, not oxidation, free from extraneous odour, no solvent residue.The Ganoderma spore extract can be applicable to oncotherapy and as having no side effect cancer therapy drug exploitation, and the inhibitor that can be used as a kind of telomerase activation is applied to clinical treatment.
The inventive method concrete steps are as follows:
One, raw material: gather Ganoderma spore and, preferably guarantee to select for use the Ganoderma spore of mature and plump through further screening.Breaking cellular wall: adopt common industrial machinery micronizing, or roll, or the physics breaking method such as grind and carry out the ultra micro breaking cellular wall, the equipment of selecting for use rolls breaking cellular wall, grinds breaking cellular wall, shear the barreling breaking cellular wall, the micronizing breaking cellular wall, ultrahigh speed airflow collision breaking cellular wall, super-voltage micro jet breaking cellular wall, type equipment such as pulverizing at ultralow temperature breaking cellular wall make breaking trachytectum of glossy ganoderma.The chitinase that can also before breaking cellular wall, in Ganoderma spore, add common commercial Application, hydrolytic enzyme such as cellulase, keep suitable condition of enzymolysis such as moisture, temperature, pH, enzymolysis time etc., make sporoderm softening and lose toughness, again through the mechanical means breaking cellular wall by enzymolysis.
Sporoderm-broken Ganoderma spore through enzymolysis separates moisture by high speed centrifugation device or ultrafiltration membrane device.The rotating speed of centrifugal device is 3000r/min～30000r/min; Ultrafiltration membrane device filter opening molecular weight is 10000.
Two, supercritical CO
2Fluid extraction: sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, make it fully contact, dissolve, extract, separate with supercritical fluid, its process conditions are set as follows: extracting pressure is 5MPa～60MPa; Extraction temperature is 32 ℃～85 ℃; CO
2Fluid flow is 5kg/h～80kg/h; The extraction time is 0.5h～6h.Extraction process can add entrainer, and as ethanol, water etc., addition is for dropping into 2%～200% of material quantity.
Three, collect extract, the ethanol or the water that will add entrainer through the high speed centrifugation device separate with extract, and the rotating speed of centrifugal device is 3000r/min～30000r/min.
The sebaceous effective active matter of lucid ganoderma spore that is of the inventive method extraction accounts for 37%～37.6% of the preceding Ganoderma spore gross weight of extraction.
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
1. screen the Ganoderma spore 100kg of mature and plump.Through the supermicro mill breaking cellular wall, the microscopic examination sporoderm-broken rate reaches 99.6%.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device.The extraction process condition is: pressure is 35MPa; Temperature is 50 ℃; CO
2Fluid flow is 25kg/h; The extraction time is 3h.
3. extract is weighed, being sebaceous active substance weight is 37kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour.
Embodiment two:
1. screen the Ganoderma spore 100kg of mature and plump.Add chitinase 0.01kg, cellulase 0.02kg, distilled water 200kg.Enzymatic hydrolysis condition is: 45 ℃ of temperature, and pH is 6.2, enzymolysis time is 1h.Sporoderm tough and tensile in enzymolysis process loses toughness gradually.Through further adopting the super micron mill broken wall treatment, wall breaking rate of ganoderma lucidum spores is 99.9%.
Sporoderm-broken Ganoderma spore through enzymolysis separates moisture by the high speed centrifugation device, and the rotating speed of centrifugal device is 3000r/min.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, add 90% ethanol 10kg as entrainer, the extraction process condition is: pressure 30MPa; Temperature is 41 ℃; CO
2Fluid flow is 5kg/h; The extraction time is 5h.
3. the ethanol that will add as entrainer through the high speed centrifugation device separates with extract, and the rotating speed of centrifugal device is 3000r/min.
4. extract is weighed, being sebaceous active substance weight is 37.1kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour, no solvent residue.
Embodiment three:
1. screen the Ganoderma spore 100kg of mature and plump.Add chitinase 0.02kg, cellulase 0.01kg, distilled water 250kg, enzymatic hydrolysis condition is: 48 ℃ of temperature, pH is 6.5, enzymolysis time is 2h.Ganoderma spore sporoderm tough and tensile in enzymolysis process loses toughness gradually.Roll broken broken wall treatment through further adopting, make breaking trachytectum of glossy ganoderma.
Sporoderm-broken Ganoderma spore through enzymolysis separates moisture by the ultrafiltration membrane device.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, the extraction process condition is: pressure is 40MPa; Temperature is 32 ℃; CO
2Fluid flow is 10kg/h; The extraction time is 4h.
3. extract is weighed, being sebaceous active substance weight is 37.3kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour.
Embodiment four:
1. screen the Ganoderma spore 100kg of mature and plump.Through ultrahigh speed airflow collision broken wall treatment, the microscopic examination sporoderm-broken rate reaches 99.6%.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, add dehydrated alcohol 2kg as entrainer, the extraction process condition is: pressure is 10MPa; Temperature is 35 ℃; CO
2Fluid flow is 12kg/h; The extraction time is 6h.
3. the ethanol that will add as entrainer through the high speed centrifugation device separates with extract, and the rotating speed of centrifugal device is 5000r/min.
4. extract is weighed, being sebaceous active substance weight is 37.2kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour, no solvent residue.
Embodiment five:
1. screen the Ganoderma spore 100kg of mature and plump.Add chitinase 0.03kg, distilled water 250kg.Enzymatic hydrolysis condition is: 45 ℃ of temperature, and pH is 6.2, enzymolysis time is 2.5h.Sporoderm tough and tensile in enzymolysis process loses toughness gradually.Shear the barreling broken wall treatment through further adopting, wall breaking rate of ganoderma lucidum spores is 99.9%.
Sporoderm-broken Ganoderma spore through enzymolysis separates moisture by the high speed centrifugation device, and the rotating speed of centrifugal device is 15000r/min.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, add distilled water 50kg as entrainer, the extraction process condition is: pressure is 50MPa; Temperature is 60 ℃; CO
2Fluid flow is 40kg/h; The extraction time is 2.5h.
3. the water that will add as entrainer through the high speed centrifugation device separates with extract, and the rotating speed of centrifugal device is 10000r/min.
4. extract is weighed, being sebaceous active substance weight is 37.6kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour.
Embodiment six:
1. screen the Ganoderma spore 100kg of mature and plump.Through the micronizing broken wall treatment, the microscopic examination sporoderm-broken rate reaches 99.7%.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, add 95% ethanol 100kg as entrainer, the extraction process condition is: pressure is 55MPa; Temperature is 68 ℃; CO
2Fluid flow is 70kg/h; The extraction time is 2h.
3. the ethanol that will add as entrainer through the high speed centrifugation device separates with extract, and the rotating speed of centrifugal device is 20000r/min.
4. extract is weighed, being sebaceous active substance weight is 37.5kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour, no solvent residue.
Embodiment seven:
1. screen the Ganoderma spore 100kg of mature and plump.Add cellulase 0.03kg, distilled water 200kg.Enzymatic hydrolysis condition is: 40 ℃ of temperature, and pH is 5.8, enzymolysis time is 1.5h.Sporoderm tough and tensile in enzymolysis process loses toughness gradually.Through rolling break process, wall breaking rate of ganoderma lucidum spores is 99.8%.
Sporoderm-broken Ganoderma spore through enzymolysis separates moisture by the high speed centrifugation device, and the rotating speed of centrifugal device is 20000r/min.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, the extraction process condition is: pressure is 60MPa; Temperature is 75 ℃; CO
2Fluid flow is 80kg/h; The extraction time is 0.5h.
3. extract is weighed, being sebaceous active substance weight is 37.4kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour, no solvent residue.
Embodiment eight:
1. screen the Ganoderma spore 100kg of mature and plump.Through shearing the barreling broken wall treatment, the microscopic examination sporoderm-broken rate reaches 99.9%.
Sporoderm-broken Ganoderma spore through enzymolysis separates moisture by the high speed centrifugation device, and the rotating speed of centrifugal device is 30000r/min.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, add 90% ethanol 100kg as entrainer, the extraction process condition is: pressure is 15MPa; Temperature is 48 ℃; CO
2Fluid flow is 25kg/h; The extraction time is 2h.
3. the ethanol that will add as entrainer through the high speed centrifugation device separates with extract, and the rotating speed of centrifugal device is 25000r/min.
4. extract is weighed, being sebaceous active substance weight is 37.5kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour, no solvent residue.
Embodiment nine:
1. screen the Ganoderma spore 100kg of mature and plump.Add chitinase 0.01kg, cellulase 0.01kg, distilled water 220kg.Enzymatic hydrolysis condition is: 45 ℃ of temperature, and pH is 7.3, enzymolysis time is 1.5h.Sporoderm tough and tensile in enzymolysis process loses toughness gradually.Through adopting ultra micro to roll broken wall treatment, wall breaking rate of ganoderma lucidum spores is 99.9%.
Sporoderm-broken Ganoderma spore through enzymolysis separates moisture by the ultrafiltration membrane device.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, add distilled water 10kg as entrainer, the extraction process condition is: pressure is 5MPa; Temperature is 85 ℃; CO
2Fluid flow is 35kg/h; The extraction time is 1.5h.
3. the water that will add as entrainer through the high speed centrifugation device separates with extract, and the rotating speed of centrifugal device is 30000r/min.
4. extract is weighed, being sebaceous active substance weight is 37.1kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour.
Embodiment ten:
1. screen the Ganoderma spore 100kg of mature and plump.Handle through super-voltage micro jet, the microscopic examination sporoderm-broken rate reaches 99.9%.
2. sporoderm-broken Ganoderma spore is put into supercritical CO
2In the extraction kettle of fluid extraction device, add 80% ethanol 200kg as entrainer, the extraction process condition is: pressure is 14MPa; Temperature is 40 ℃; CO
2Fluid flow is 12kg/h; The extraction time is 0.5h.
3. the ethanol that will add as entrainer through the high speed centrifugation device separates with extract, and the rotating speed of centrifugal device is 6000r/min.
4. extract is weighed, being sebaceous active substance weight is 37.4kg.Be transparent grease after testing, do not have precipitation, not oxidation has the peculiar fragrance of Ganoderma spore, free from extraneous odour, no solvent residue.
Claims (6)
1. an extracting method for effective active matter of lucid ganoderma spore is characterized in that Ganoderma spore is through breaking cellular wall, supercritical CO
2Fluid extraction and extract separation circuit are handled.
2. the method for claim 1 is characterized in that selecting for use the Ganoderma spore of mature and plump.
3. the method for claim 1 adopts mechanical micronizing, or rolls when it is characterized in that breaking cellular wall, or ground physics breaking method carries out the ultra micro breaking cellular wall.
4. the method for claim 1 adds chitinase and/or cellulase when it is characterized in that breaking cellular wall.
5. the method for claim 1 is characterized in that supercritical CO
2Fluid extraction pressure is 5MPa～60MPa, and extraction temperature is 32 ℃～85 ℃, CO
2Fluid flow is 5kg/h～80kg/h, and the extraction time is 0.5h～6h.
6. the method for claim 1 is characterized in that at supercritical CO
2The fluid extraction process adds entrainer ethanol and/or water, and addition is for dropping into 2%～200% of material quantity.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00117466 CN1114446C (en) | 2000-10-09 | 2000-10-09 | Extracting method for effective active matter of lucid ganoderma spore |
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| CN 00117466 CN1114446C (en) | 2000-10-09 | 2000-10-09 | Extracting method for effective active matter of lucid ganoderma spore |
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| CN1290554A true CN1290554A (en) | 2001-04-11 |
| CN1114446C CN1114446C (en) | 2003-07-16 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1245235A3 (en) * | 2001-03-19 | 2002-10-09 | Xin Liu | Method for extracting oleaginous substances from ganoderma lucidum spores |
| CN100339089C (en) * | 2003-10-14 | 2007-09-26 | 上海中祥生物制品有限公司 | Method for extracting ganoderma lucidum oil from ganoderma lucidum mycelium |
| WO2007134548A1 (en) * | 2006-05-24 | 2007-11-29 | Guangdong Yuewei Edible Fungi Technology Co. Ltd | A process for preparing ganoderma spore oil |
| CN101028305B (en) * | 2007-03-23 | 2010-08-25 | 卢昶年 | Ganoderic spore extract and its production |
| CN102204942A (en) * | 2011-05-24 | 2011-10-05 | 黑龙江大学 | Method for extracting corylus heterophylla leaf flavone |
| CN101176548B (en) * | 2007-11-30 | 2012-05-09 | 华南理工大学 | Method for preparing low acid value ganoderma lucidum spore oil |
| CN103351941A (en) * | 2013-06-17 | 2013-10-16 | 浙江龙泉佳宝生物科技有限公司 | Supercritical CO2 variable-temperature variable-pressure extraction method of lucid ganoderma spore oil |
| CN104137979A (en) * | 2014-07-29 | 2014-11-12 | 桂林实力科技有限公司 | Fructus momordicae aspartame and preparation method thereof |
| CN105219647A (en) * | 2015-10-23 | 2016-01-06 | 南京中医药大学 | A kind of wall-breaking method of Ganoderma spore |
| CN112779086A (en) * | 2020-12-18 | 2021-05-11 | 江苏滋补堂药业有限公司 | Method for removing peroxide value of ganoderma lucidum spore oil |
| CN113684088A (en) * | 2020-05-18 | 2021-11-23 | 嘉必优生物技术（武汉）股份有限公司 | Microbial oil extraction method and microbial oil obtained by same |
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| CN1308011C (en) * | 2003-12-22 | 2007-04-04 | 中国科学院微生物研究所 | Method for extracting ganoderma lucidum spore fat soluble active substance and its specified instrument for preparation |
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2000
- 2000-10-09 CN CN 00117466 patent/CN1114446C/en not_active Expired - Lifetime
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1245235A3 (en) * | 2001-03-19 | 2002-10-09 | Xin Liu | Method for extracting oleaginous substances from ganoderma lucidum spores |
| CN100339089C (en) * | 2003-10-14 | 2007-09-26 | 上海中祥生物制品有限公司 | Method for extracting ganoderma lucidum oil from ganoderma lucidum mycelium |
| WO2007134548A1 (en) * | 2006-05-24 | 2007-11-29 | Guangdong Yuewei Edible Fungi Technology Co. Ltd | A process for preparing ganoderma spore oil |
| CN101028305B (en) * | 2007-03-23 | 2010-08-25 | 卢昶年 | Ganoderic spore extract and its production |
| CN101176548B (en) * | 2007-11-30 | 2012-05-09 | 华南理工大学 | Method for preparing low acid value ganoderma lucidum spore oil |
| CN102204942B (en) * | 2011-05-24 | 2012-12-05 | 黑龙江大学 | Method for extracting corylus heterophylla leaf flavone |
| CN102204942A (en) * | 2011-05-24 | 2011-10-05 | 黑龙江大学 | Method for extracting corylus heterophylla leaf flavone |
| CN103351941A (en) * | 2013-06-17 | 2013-10-16 | 浙江龙泉佳宝生物科技有限公司 | Supercritical CO2 variable-temperature variable-pressure extraction method of lucid ganoderma spore oil |
| CN103351941B (en) * | 2013-06-17 | 2015-06-17 | 浙江龙泉佳宝生物科技有限公司 | Supercritical CO2 variable-temperature variable-pressure extraction method of lucid ganoderma spore oil |
| CN104137979A (en) * | 2014-07-29 | 2014-11-12 | 桂林实力科技有限公司 | Fructus momordicae aspartame and preparation method thereof |
| CN104137979B (en) * | 2014-07-29 | 2015-12-02 | 桂林实力科技有限公司 | A kind of mangosteen sweetener and preparation method thereof |
| CN105219647A (en) * | 2015-10-23 | 2016-01-06 | 南京中医药大学 | A kind of wall-breaking method of Ganoderma spore |
| CN113684088A (en) * | 2020-05-18 | 2021-11-23 | 嘉必优生物技术（武汉）股份有限公司 | Microbial oil extraction method and microbial oil obtained by same |
| CN113684088B (en) * | 2020-05-18 | 2024-06-11 | 嘉必优生物技术（武汉）股份有限公司 | Microbial oil extraction method and microbial oil obtained by same |
| CN112779086A (en) * | 2020-12-18 | 2021-05-11 | 江苏滋补堂药业有限公司 | Method for removing peroxide value of ganoderma lucidum spore oil |
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| Publication number | Publication date |
|---|---|
| CN1114446C (en) | 2003-07-16 |
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