CN1290522C - Immunity regulating medicine - Google Patents
Immunity regulating medicine Download PDFInfo
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- CN1290522C CN1290522C CN 200410040203 CN200410040203A CN1290522C CN 1290522 C CN1290522 C CN 1290522C CN 200410040203 CN200410040203 CN 200410040203 CN 200410040203 A CN200410040203 A CN 200410040203A CN 1290522 C CN1290522 C CN 1290522C
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- wasabia japonic
- euterma wasabi
- medicine
- extract
- isothiocyanate
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Abstract
The present invention relates to a medicine which has the function of regulating the immunity. Oily mixture obtained by extracting horseradish which is taken as raw materials is used as an effectively medicine component, and the medicine also contains secondary components which is acceptable in the medicine.
Description
Technical field
The present invention relates to a kind ofly have application in the immunoregulation effect medicine in preparation with natural plants Wasabia japonic (Euterma Wasabi) extract.
Background technology
Wasabia japonic (Euterma Wasabi) (Wasabia japonica Matsum) has another name called scurvy grass, for the Cruciferae Wasabia japonic (Euterma Wasabi) belongs to herbaceos perennial, and the subterraneous stem hypertrophy, cylindrical, the skin green, marrow is white partially, and meat is hard, has the fragrant pungent of volatility awns after grinding.Wasabia japonic (Euterma Wasabi) originates in Japan, is the very high vegetable of a kind of economic worth medicinal plants of holding concurrently.For hundreds of years as the pungent material of traditional flavoring agent.Known now, glucose isocyanates (Glucosinolate) and catabolite thereof in the Wasabia japonic (Euterma Wasabi) are the sources of special acid, this is the chemical compound of a class sulfur-bearing, and its common basic structure is a β-D-thioglucose group and variable side chain (can derived from methionine, tryptophan, phenylalanine and some branched chain amino acid).Wherein different side chain R makes them have different separately characteristics.The glucose isothiocyanate is present in the organelle, and chemical property own is stable, there is no biological activity.Have only when plant tissue is damaged cell disruption, under the effect of endogenous myrosinase, hydrolysis generates glucose and various volatile products, and isothiocyanate may be wherein most important and the catabolite of research fullest.The mutual transformation process of its glucose isothiocyanate, isothiocyanate is as follows:
Sultana, Tamanna; McNeil, David L; Porter, Noel G.; Savage, Geoffrey P.Investigationof isothiocyanate yield from flowering and non-flowering tissues of wasabi grown in aflooded system. Journal of Food Composition and Analysis Volume:16, Issue:6, December, 2003, report among the pp 637-646, in all isosulfocyanate compounds of Wasabia japonic (Euterma Wasabi), the content of allyl group isosulfocyanate (Allyl Isothiocyanate) is about 89-94%, be the highest a kind of composition of content wherein, at petiole, stem and root are particularly abundant.The higher composition of another content is 3-cyclobutenyl isothiocyanate (3-Butenyl Isothiocyanate) in the pungent substance, and other only account for the 1.4-3.5% of isothiocyanate total content as 4-pentenyl (4-pentenyl), isopropyl (iso-propyl), dibutyl (sec-butyl), isobutyl group (iso-butyl) isothiocyanate etc.Wasabia japonic (Euterma Wasabi) each several part volatile sulfur compound and content are as shown in table 1.
Table 1 Wasabia japonic (Euterma Wasabi) each several part volatile sulfur compound and content
| Peak No. | Compound(NCS＝isothiocyanate) | Leaf | g/100g essential oil | ||
| Petiole | Rhizome | Root | |||
| l 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 | 1-Penten-3-ol 3-Butenonitrile Isopropyl NCS trans-2-Hexenal sec-Butyl NCS cis-2-Penten-1-ol Isobutyl NCS I-Hexanol Allyl NCS Unknown trans-2-Hexen-1-ol 3-Butenyl NCS 4-Pentenyl NCS 5-Hexenyl NCS 6-Heptenyl NCS Unknown 4-Methylthiobutanonitrile 3-Methylthiopropyl NCS 6-Methythiohexanonitrile 4-Methylthiobutyl NCS 7-Methylthioheptanonitrile 5-Methylthiopeotyl NCS 6-Methylthiohexyl NCS 7-Methylthioheptyl NCS | 0.63 2.35 0.33 14.94 0.81 0.31 1.05 0.03 55.07 14.60 0.51 4.29 0.45 - - 2.65 0.09 0.39 0.14 - 0.83 0.08 0.45 0.16 | - 3.11 - - 1.94 0.27 0.09 0.01 79.57 - 0.04 8.42 1.27 0.18 0.14 - 0.01 0.36 0.11 - 0.47 0.21 1.66 2.10 | 0.01 0.87 - - 0.6 0.02 0.03 0.14 83.26 - 0.54 6.96 3.65 0.94 0.06 - - 0.13 0.01 0.01 0.02 0.58 1.98 0.18 | - 4.39 - - 4.39 0.03 0.11 0.09 79.39 - 0.09 4.44 0.73 0.18 0.95 0.08 0.04 0.41 0.17 0.15 0.79 0.36 2.49 0.73 |
| Total mg oil/100g plant(six measurements) | 28.65±3.65 | 16.15±0.45 | 158.02±13.42 | 20.52±2.52 | |
At A.Depree, J.; M.Howard, T.; P.Savage, G.Flavour and pharmaceutical properties ofthe volatile sulphur compounds of Wasabi (Wasabia japonica) Food ResearchInternational.Vo1ume:31, Issue:5, June, 1998, among the pp329-337, people such as Kumagai also confirm, the Wasabia japonic (Euterma Wasabi) root is rich in 6-methyl mercapto hexyl (6-methylthiohexyl) and 7-methyl mercapto heptyl (7-methylthioheptyl) isothiocyanate, the 6-of stem methyl mercapto hexyl isothiocyanate content is higher, and the 7-of petiole portion methyl mercapto heptyl isothiocyanate content is higher, and each compound concentrations is relatively low in the blade.In the above chemical compound, the inferior sulfide of first is that Wasabia japonic (Euterma Wasabi) and Radix Cochleariae officinalis all contain, and the first sulfide peculiar piquancy component that is Wasabia japonic (Euterma Wasabi), its content be Radix Cochleariae officinalis 200-400 doubly.
Recently discover: it not only can be used as food, now discovers and reports, main piquancy component wherein---and isothiocyanate (Isothiocyanate) has more multiple pharmacological effect such as antibiotic, anticancer, antioxidation.As, people such as Hecht are at Tanida N.Kawaura A.Takahashi A.Sawada K.Shimoyama T.Suppressive effect ofwasabi (pungent Japanese spice) on gastric carcinogenesis induced by MNNG in rats.[Journal Article] Nutrition﹠amp; Cancer.16 (1): 53-8,1991., and Huang is built, Sun Jing is at " bioavailability of glucose isothiocyanate reaches the meaning to health " (" foreign medical science: hygiology fascicle " 2003,30 (2) .-94-97) report respectively in, isothiocyanates such as phenethyl isothiocyanate (PEITC), benzyl isothiocyanate (BITC) and sulforaphane can change the I that expresses in liver and the epithelial cell mutually with the balance of the mutually exogenous carcinogen associated metabolic of II enzyme.Isothiocyanate can be prevented the cytochrome P 450 enzymes in the I phase metabolism, promote the NADPH in the II phase metabolism (to comprise quinone reductase QR, the UDP-glucronyl transferring enzyme), consequently increases carcinogen detoxifcation and drainage, reduce the damaging action of carcinogen then DNA.
Watanabe M etc. are at Watahabe M.Ohata M.Hayakawa S.Isemura M.Kumazawa S.Nakayama T.Furugori M.Kinae N.Identification of 6-methylsulfinylhexylisothiocyanate as an apoptosis-inducing component in wasabi.[Journal Article] Phytochemistry.62 (5): 733-9, confirm also in the report of 2003 Mar. that the 6-first sulfurous base hexyl isothiocyanate in the Wasabia japonic (Euterma Wasabi) ethanol extract suppresses the growth of monocytic leukemia U937 cell and gastric cancer MKN45 cell and induces its apoptosis external, suppress cell proliferation.
In addition, Pedras MS.Sorensen JL.Okanga FI.Zaharia IL. Wasalexins A and B, newphytoalexins from wasabi:isolation, synthesis, and antifungal activity.[Journal Article] Bioorganic﹠amp; Medicinal Chemistry Letters.9 (20): 3015-20,1999 Oct 18. and Ono H.TesakiS.Tanabe S.Watanabe be isothiocyanate and its homologues asfood-originated compounds with antibacterial activity against Escherichia coli andStaphylococcus aureus.[Journal Article M.6-Methylsulfinylhexyl] Bioscience, Biotechnology﹠amp; Biochemistry.62 (2): 363-5, some documents such as 1998 Feb. have confirmed that also isothiocyanate can both have very strong sterilizing power to various bacteria such as colon bacillus genus, Salmonella, staphylococcus aureuses.
Wasabia japonic (Euterma Wasabi) also has the effect of antiplatelet aggregation, render a service required isothiocyanic acid ester concentration and be about 10 times of aspirin though there is report to reach its pharmacology of 1/2nd, its effect rapidly, aspirin then needs the onset of 30 minutes ability.Morimitsu R studies confirm that further the Wasabia japonic (Euterma Wasabi) extracting solution all has obvious antiplatelet aggregative activity in vivo and in vitro, and its mechanism may induce glutathione transfers enzyme activity relevant with 6-HITC.
At present the antioxidant effect of crucifers such as Wasabia japonic (Euterma Wasabi) is extensively admitted.This is closely related with wherein phenethyl isothiocyanate and sulforaphane.And oxidation has caused concern to the damage that histoorgan causes in cancer, the aging effect that reaches in some chronic diseases, and antioxidant can be in the generation that to a certain degree delays even suppress this process.
Some documents report that also sulfur-containing compounds such as benzyl (benzyl) and allyl group isosulfocyanate also can have antasthmatic effect, reach the effect of aspects such as parasiticide.
Summary of the invention
On the basis of foregoing, the present invention will further provide with the medicine with immunoregulation effect of Wasabia japonic (Euterma Wasabi) extract as the active drug composition.
Medicine with immunoregulation effect of the present invention to be that the oily mixture that the raw material extraction obtains is the active drug composition by Wasabia japonic (Euterma Wasabi), is formed jointly with the auxiliary element that allows in the medicine to accept.Wherein, preferably its preparation is become the medicine of oral formulations type.
Wish the Wasabia japonic (Euterma Wasabi) extract component of medicine active drug composition of the present invention, can adopt in the present document existing reported method to obtain by extracting in the Wasabia japonic (Euterma Wasabi) raw material.As instantiation, also can adopt method preparation provided by the invention.
One of preparation method:
A Wasabia japonic (Euterma Wasabi) powder 200 grams are mixed with 1700 milliliters of adding round-bottomed flasks of water, and heating also kept fluidized state about 1 hour, and filtration under diminished pressure gets the yellow green suspension, and pungent pungent taste is arranged.This oily mater is condensed into comparatively heavy-gravity oily liquid, can remakes filtration treatment in case of necessity, promptly become the said oily mixture that can be used as the use of active drug composition of the present invention and extract composition.
Two of preparation method:
Wasabia japonic (Euterma Wasabi) powder 25 grams are added 250 milliliters of ethyl acetate carry out the reflux extraction, get emerald green mixed liquor, the fragrance of ester is arranged.Cold filtration gets emerald green filtrate.With fractional distillation mode Separation and Recovery ethyl acetate, promptly obtain the said oily mixture that can be used as the use of active drug composition of the present invention and extract composition.Can add ethyl acetate in case of necessity again and do same the processing, carry out purification refine.
Extract composition with the above-mentioned Wasabia japonic (Euterma Wasabi) oily mixture that obtains and carried out following test the immunoregulation effect influence as trial drug.
One, external immunoregulatory effect
1. stimulate mouse macrophage Ana-1 to produce the test of NO.
Above-mentioned trial drug can stimulate mouse macrophage Ana-1 to produce NO (nitric oxide), variable concentrations Wasabia japonic (Euterma Wasabi) extract trial drug stimulates Ana-1 to produce NO ability difference, high concentration (C1 group: 5mg crude drug/ml) greater than middle concentration (the C2 group: 0.5mg crude drug/ml), greater than low concentration (C3 group: 0.05mg crude drug/ml); C1 concentration group stimulates ability the strongest, reduces the thorn ability drop with concentration, and C3 concentration group growth curve is tending towards normal control.The OD value comparative result of NO as shown in Figure 1 in the Wasabia japonic (Euterma Wasabi) extract stimulation mouse macrophage Ana-1 different time culture supernatant of variable concentrations.
2. stimulate mouse macrophage Ana-1 to produce the test of IL-12 (interleukin 12).
Above-mentioned trial drug can stimulate mouse macrophage Ana-1 to produce IL-12, and variable concentrations Wasabia japonic (Euterma Wasabi) extract trial drug stimulates Ana-1 to produce IL-12 ability difference, and (the C1 group: 5mg crude drug/ml) is greater than middle concentration (C2 group: 0.5mg crude drug/ml) for high concentration.Low concentration (C3 group: 0.05mg crude drug/ml) no effect.The OD value comparative result of IL-12 as shown in Figure 2 in the Wasabia japonic (Euterma Wasabi) extract trial drug stimulation mouse macrophage Ana-1 different time culture supernatant of variable concentrations.
3. stimulate mouse macrophage Ana-1 to produce the test of TNF-α (tumor necrosis factor-alpha).
Wasabia japonic (Euterma Wasabi) extract trial drug can stimulate mouse macrophage Ana-1 to produce TNF-α, variable concentrations Wasabia japonic (Euterma Wasabi) extract trial drug stimulates Ana-1 to produce IL-12 ability difference, and (C1:5mg crude drug/ml) is greater than middle concentration (C2:0.5mg crude drug/ml) for high concentration.Low concentration (C3 0.05mg) crude drug/ml) no effect.The OD value comparative result of TNF as shown in Figure 3 in the Wasabia japonic (Euterma Wasabi) extract trial drug stimulation mouse macrophage Ana-1 different time culture supernatant of variable concentrations.
Two, immunoregulation effect test in the body.
Adopt bleomycin to set up the lung fibrosis in rats animal model, study Wasabia japonic (Euterma Wasabi) extract trial drug of the present invention pulmonary inflammatory regulating action.Wasabia japonic (Euterma Wasabi) extract trial drug of the present invention and blank group are to lung tissue INF-γ (gamma interferon), and the comparative test result photo of the expression of IL-2 (interleukin II) and lL-12 is respectively as Fig. 4, Fig. 5 and shown in Figure 6.Picture A among each figure is the blank group respectively, and photo B is respectively Wasabia japonic (Euterma Wasabi) extract trial drug of the present invention.Each organize dark display part among the photo B show respectively INF-γ (gamma interferon) is arranged, the expression of IL-2 (interleukin II) and IL-12 (interleukin 12).Because INF-γ, IL-2 and IL-12 are the cytokine of participation immunologic function, so this immunohistochemistry result's test shows that Wasabia japonic (Euterma Wasabi) extract of the present invention has immunoregulation effect and function in the significant body.
With this extract component is the active drug composition, with other the auxiliary adding ingredient that allows in the medicine to accept, for example with oral formulations in can received disintegrating agent, after auxiliary interpolation composition that excipient, lubricant, binding agent, filler etc. are commonly used mixes, handle by corresponding common process method, promptly may be made in as solid oral medicine preparations such as tablet, pill, capsules.Or mix with commonly used surfactants such as solubilizing agent, emulsifying agent, wetting agent, foaming or defoamer, diluent, antiseptic, stabilizing agent, correctives, thickening agent etc., handle by corresponding common process method, promptly may be made in the oral drugs of liquid preparation forms such as water preparation, syrup.Because the mode of these preparations all belongs to present mature technology, need not too much to give unnecessary details.
Description of drawings
Fig. 1 is the OD value comparative result that the Wasabia japonic (Euterma Wasabi) extract trial drug of variable concentrations stimulates NO in the mouse macrophage Ana-1 different time culture supernatant
Fig. 2 is the OD value comparative result that the Wasabia japonic (Euterma Wasabi) extract trial drug of variable concentrations stimulates IL-12 in the mouse macrophage Ana-1 different time culture supernatant.
Fig. 3 is the OD value comparative result that the Wasabia japonic (Euterma Wasabi) extract trial drug of variable concentrations stimulates TNF in the mouse macrophage Ana-1 different time culture supernatant.
Fig. 4 is that invention Wasabia japonic (Euterma Wasabi) extract trial drug increases the comparative test result that lung tissue INF-γ expresses.
Fig. 5 is that invention Wasabia japonic (Euterma Wasabi) extract trial drug increases the comparative test result that lung tissue IL-2 expresses.
Fig. 6 is that invention Wasabia japonic (Euterma Wasabi) extract trial drug increases the comparative test result that lung tissue IL-12 expresses.
Claims (2)
1. the Wasabia japonic (Euterma Wasabi) extract has application in the immunoregulation effect medicine in preparation, it is characterized in that with by Wasabia japonic (Euterma Wasabi) be raw material to extract the oily mixture that obtains be the active drug composition, with the auxiliary element mixed together preparation that allows in the medicine to accept.
2. application as claimed in claim 1 is characterized in that preparing the medicine that becomes oral formulations.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410040203 CN1290522C (en) | 2004-07-13 | 2004-07-13 | Immunity regulating medicine |
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| CN 200410040203 CN1290522C (en) | 2004-07-13 | 2004-07-13 | Immunity regulating medicine |
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| CN1290522C true CN1290522C (en) | 2006-12-20 |
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| RU2452242C1 (en) * | 2011-02-22 | 2012-06-10 | Сергей Анатольевич Шеремет | Biologically active food additive production method |
| CN103251672B (en) * | 2013-05-30 | 2015-03-25 | 成都大学 | Horseradish oral spray and preparation method thereof |
| CN104382035B (en) * | 2014-12-04 | 2017-10-10 | 李楠 | Double-deck horseradish buccal tablet for preventing respiratory allergy |
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