CN1286889C - Chip simply non-reversion binding method using poly dimethyl siloxanes as substrate material - Google Patents
Chip simply non-reversion binding method using poly dimethyl siloxanes as substrate material Download PDFInfo
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- CN1286889C CN1286889C CN 200510009804 CN200510009804A CN1286889C CN 1286889 C CN1286889 C CN 1286889C CN 200510009804 CN200510009804 CN 200510009804 CN 200510009804 A CN200510009804 A CN 200510009804A CN 1286889 C CN1286889 C CN 1286889C
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Abstract
The present invention provides a simple chip irreversible bonding method using polydimethyl siloxane as a substrate material. The method mainly comprises the steps that the substrate material of a chip is polydimethyl siloxane; the polydimethyl siloxane is poured in a mould and is solidified, and the fresh polydimethyl siloxane sample is stripped; the stripped fresh polydimethyl siloxane chip assembly is placed in ammonia water and is soaked; the treated polydimethyl siloxane chip assembly is repeatedly flushed by deionized water; two treated polydimethyl siloxane chip assemblies are attached to each other, or one treated polydimethyl siloxane chip assembly is pasted on a cleaned glass surface, so that the polydimethyl siloxane is irreversibly bonded with polydimethyl siloxane or glass. In the present invention, polydimethyl siloxane is adopted as a substrate material for processing, and the present invention has the advantages of high performance-price ratio, low bonding cost, simple processing process and high processing rate of finished products.
Description
(1) technical field
The present invention relates to be a kind of be the simple and easy bonding method of the chip of base material with polydimethylsiloxane (PDMS).
(2) background technology
Micro-fluidic chip (Micro-fluidic chip) is micro-total analysis system (Micrototalanalysis system) or the important component part that claims chip lab (Lab-on-a-chip), has caused people's common concern and interest.Present stage, made chip adopted biotechnology, microelectronics and MEMS technology more, had been that substrate is processed various working cells with base materials such as silicon chip, glass with the IC technical process of standard, i.e. photoetching-corrode-get through hole-bonding.But the photoetching and the etching technique technology of this method making micro-fluidic chip are tediously long, time-consuming, need super-clean environment, and the chip bonding difficulty is big, and material brittle and cost are higher.Therefore, high molecular polymer such as polymethylmethacrylate (Polymethylmethacrylate, PMMA), and polydimethylsiloxane (Polydimethylsiloxane, HO (SiC
2H
4O)
nH PDMS), become the important materials of making micro-fluidic chip.
Polydimethylsiloxane is a kind of rubber-like high molecular polymer, and it is to be formed by PDMS matrix and corresponding curing agent thermopolymerization according to a certain percentage usually.As the base material that makes up micro-fluidic chip, PDMS has shown very desirable material characteristic: good insulation performance, can bear high-voltage, and be widely used in the making of various capillary electrophoresis microchips; The thermostability height is fit to the various biochemical reaction chips of processing, has very high bio-compatibility and gas permeability, can be used for cell cultures; Simultaneously, have good optical characteristics, can be applicable to multiple optical detection system; Young's modulus is low, is suitable for making the microfluidic control device, as capillary electrophoresis chip, Micropump etc.
PDMS can see through 300nm above ultraviolet and visible light, and has characteristics such as nontoxic, easy with the processing of casting reproducible microchannel, that chip cost is low, is to use more high polymer material in the microfluidic analysis chip preparation.In addition, PDMS also has the following advantages: the prices of raw and semifnished materials are cheap, fabrication cycle is short, wearing quality is good, method for packing is flexible, can form good sealing with many materials such as silicon, silicon nitride, silicon oxide, glass.
At present, the PDMS chip adopts two kinds of bonding modes usually: reversible keying and irreversible bonding.The reversible keying method is that two fresh PDMS chips of peeling off are directly fitted, and be bonded together after handling through insulation, but the intensity of bonding is lower, is prone to the phenomenon of leakage in the process of using; Irreversible bonding method adopts oxygen plasma treatment PDMS surface, or with uviolizing PDMS surface, then two PDMS chips is directly fitted, and realizes irreversible bonding afterwards through processing such as insulations again.Be plasma mode or carrying out bonding with ultraviolet method all needs specific equipment, and the integral body of equipment required than higher that common laboratory is difficult to realize.
(3) visitor in the invention
The object of the present invention is to provide that a kind of technological process is simple, cost is low, production efficiency is high, what help improving the quality of products is the simple and easy irreversible bonding method of chip of base material with the polydimethylsiloxane.
The object of the present invention is achieved like this:
(1) substrate material of chip is polydimethylsiloxane and polydimethylsiloxane, or polydimethylsiloxane and glass;
(2) polydimethylsiloxane performed polymer and solidifying agent are mixed in proportion, are cast in the mould, solidify, peel off the polydimethylsiloxane sample is fresh again, obtain the polydimethylsiloxane assembly;
(3) the fresh polydimethylsiloxanechip chip assembly of peeling off is put into ammoniacal liquor, soaked 10～30 minutes, take out;
(4) polydimethylsiloxanechip chip assembly is after treatment washed repeatedly with deionized water;
(5) after two polydimethylsiloxanechip chip component joint will handling well,, realize the irreversible bonding of polydimethylsiloxane and polydimethylsiloxane in 80～150 ℃ of insulations 1～3 hour; Or the polydimethylsiloxanechip chip assembly of handling well is attached on the glass surface that cleans up, 80～150 ℃ of insulations 1～3 hour realize the irreversible bonding of polydimethylsiloxane and glass.
The present invention can also comprise some features like this:
1, the polydimethylsiloxane performed polymer is 4-6 with the mixed volume ratio of solidifying agent polymkeric substance: 1.
2, the weight ratio concentration of described ammoniacal liquor is 15%～28%.
3, solidification value is 25～125 ℃.
Bonding principle of the present invention:
PDMS surface after the polymerization is mainly-Si (CH
3)
3Form exist, after ammonia soln is handled, produce a large amount of Si-OH keys on its surface, after the on-the-spot applying of PDMS chip after the processing, insulation through certain temperature is handled, and just can produce a large amount of Si-O-Si keys between two chips, thereby realizes the irreversible bonding of chip.And mainly contain SiO in the glass
2, glass and fit with the PDMS chip after the ammonia treatment is on-the-spot after, handle through the insulation of certain temperature, just can between the PDMS/Glass interface, form a large amount of hydrogen bond Si-OH----O-Si, make its bonding, but that bond strength is not so good as PDMS/PDMS is firm.
The invention has the advantages that: 1, adopting polydimethylsiloxane is the process substrate material, being that the chip of substrate material has than high performance-price ratio selecting for use of material with silicon chip, glass; 2, adopt the ammonia treatment surface, do not need special plant and instrument and expensive reagent, the bonding cost is reduced greatly; 3, this bonding method does not need complicated processing unit, and machining process is simplified, and cost of manufacture reduces, processed finished products rate height.
(4) description of drawings
Fig. 1 is a PDMS chip bonding interface photo among the embodiment 1;
Fig. 2 is for there being the bonded interface photo of raceway groove PDMS chip among the embodiment 2;
(5) specific embodiments
For a more detailed description to the present invention for example below:
Embodiment 1:
Commercially available PDMS (polydimethylsiloxane) generally is made up of for two doses performed polymer and solidifying agent at present, at first PDMS (polydimethylsiloxane) performed polymer and solidifying agent were mixed in 5: 1 by volume, be cast in the mould respectively then, the 10min that outgases in vacuum chamber under the room temperature forms certain thickness liquid layer.Be heating and curing under 25～125 ℃ of conditions in the selected temperature scope, reach predetermined set time after, with fresh the peeling off of PDMS sheet behind the polymerizing curable, put into ammoniacal liquor, soaked 10～30 minutes, take out; Again PDMS chip assembly is after treatment washed repeatedly with deionized water, to dispel the ammoniacal liquor that is adsorbed in chip surface; Two PDMS chip assembly lower surfaces handling well to sticking, are placed 80～150 ℃ of baking ovens insulations 1～3 hour, can realize irreversible bonding.The bonding strength between two PDMS is measured in the cooling back.As can be seen from Figure 1 merge fully at two PDMS chip bonding interfaces.
Embodiment 2:
By case 1 method preparation PDMS mixed solution, be cast in respectively in the mould of raceway groove figure and no raceway groove figure, solidify; With fresh the peeling off of PDMS sheet behind the polymerizing curable, put into ammoniacal liquor, soaked 10～30 minutes, take out; Again PDMS chip assembly is after treatment washed repeatedly with deionized water, to dispel the ammoniacal liquor that is adsorbed in chip surface and the raceway groove figure; Two PDMS chip assembly lower surfaces handling well to sticking, are placed 80～150 ℃ of baking ovens insulations 1～3 hour, can realize irreversible bonding.The bonding strength between two PDMS is measured in the cooling back.
Embodiment 3:
At first PDMS (polydimethylsiloxane) matrix and solidifying agent were mixed in 4: 1 by volume, be cast in respectively then in the mould, the 10min that outgases in vacuum chamber under the room temperature forms certain thickness liquid layer.Under selected temperature scope (25～125 ℃), be heating and curing, reach predetermined set time after, with fresh the peeling off of PDMS sheet behind the polymerizing curable, put into ammoniacal liquor, soaked 10～30 minutes, take out; Again PDMS chip assembly is after treatment washed repeatedly with deionized water, to dispel the ammoniacal liquor that is adsorbed in chip surface; Two PDMS chip assembly lower surfaces handling well to sticking, are placed 80～150 ℃ of baking ovens insulations 1～3 hour, can realize irreversible bonding.The bonding strength between two PDMS is measured in the cooling back.
Embodiment 4:
At first PDMS (polydimethylsiloxane) matrix and solidifying agent were mixed in 6: 1 by volume, be cast in respectively then in the mould, the 10min that outgases in vacuum chamber under the room temperature forms certain thickness liquid layer.Under selected temperature scope (25～125 ℃), be heating and curing, reach predetermined set time after, with fresh the peeling off of PDMS sheet behind the polymerizing curable, put into ammoniacal liquor, soaked 10～30 minutes, take out; Again PDMS chip assembly is after treatment washed repeatedly with deionized water, to dispel the ammoniacal liquor that is adsorbed in chip surface; Two PDMS chip assembly lower surfaces handling well to sticking, are placed 80～150 ℃ of baking ovens insulations 1～3 hour, can realize irreversible bonding.The bonding strength between two PDMS is measured in the cooling back.
Claims (5)
1, a kind of is the simple and easy irreversible bonding method of chip of base material with the polydimethylsiloxane, it is characterized in that:
(1) substrate material of chip is polydimethylsiloxane and polydimethylsiloxane, or polydimethylsiloxane and glass;
(2) polydimethylsiloxane performed polymer and solidifying agent are mixed in proportion, are cast in the mould, solidify, peel off the polydimethylsiloxane sample is fresh again, obtain the polydimethylsiloxane assembly;
(3) the fresh polydimethylsiloxanechip chip assembly of peeling off is put into ammoniacal liquor, soaked 10～30 minutes, take out;
(4) polydimethylsiloxanechip chip assembly is after treatment washed repeatedly with deionized water;
(5) after two polydimethylsiloxanechip chip component joint will handling well,, realize the irreversible bonding of polydimethylsiloxane and polydimethylsiloxane in 80～150 ℃ of insulations 1～3 hour; Or the polydimethylsiloxanechip chip assembly of handling well is attached on the glass surface that cleans up, 80～150 ℃ of insulations 1～3 hour realize the irreversible bonding of polydimethylsiloxane and glass.
2, according to claim 1 is the simple and easy irreversible bonding method of chip of base material with the polydimethylsiloxane, it is characterized in that: the polydimethylsiloxane performed polymer is 4-6 with the mixed volume ratio of solidifying agent polymkeric substance: 1.
3, according to claim 1 and 2 is the simple and easy irreversible bonding method of chip of base material with the polydimethylsiloxane, and it is characterized in that: the weight ratio concentration of described ammoniacal liquor is 15%～28%.
4, according to claim 1 and 2 is the simple and easy irreversible bonding method of chip of base material with the polydimethylsiloxane, and it is characterized in that: solidification value is 25～125 ℃.
5, according to claim 3 is the simple and easy irreversible bonding method of chip of base material with the polydimethylsiloxane, and it is characterized in that: solidification value is 25～125 ℃.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510009804 CN1286889C (en) | 2005-03-09 | 2005-03-09 | Chip simply non-reversion binding method using poly dimethyl siloxanes as substrate material |
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| CN 200510009804 CN1286889C (en) | 2005-03-09 | 2005-03-09 | Chip simply non-reversion binding method using poly dimethyl siloxanes as substrate material |
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| CN1286889C true CN1286889C (en) | 2006-11-29 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100420622C (en) * | 2005-12-28 | 2008-09-24 | 哈尔滨工业大学 | Secondary template duplicating process method based on dimethyl silicone polymer mini component |
| CN101424660B (en) * | 2007-10-31 | 2012-01-04 | 南京大学 | Design and production of portable highly effective capillary pipe electrophoresis chip sampling by chink |
| CN102276862A (en) * | 2011-04-28 | 2011-12-14 | 西北工业大学 | Method for irreversibly bonding polymethyl methacrylate (PMMA) chip and polydimethylsiloxane (PDMS) chip |
| CN102701145A (en) * | 2012-05-04 | 2012-10-03 | 南京大学 | Method for irreversible bonding of high-quality polydimethylsiloxane (PDMS)-polyolefine plastics |
| CN103132163B (en) * | 2013-03-12 | 2016-01-27 | 东南大学 | A kind of preparation method with the fiber of multi-kernel shell structure |
| CN105498868B (en) * | 2015-11-20 | 2017-10-13 | 华南师范大学 | A kind of method for packing of micro-fluidic chip |
| CN108659539A (en) * | 2018-05-21 | 2018-10-16 | 哈尔滨工业大学 | A kind of application of stretching-sensitive type flexible sensing material preparation method and monitoring concrete deformation and crack |
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