CN1285736C - Gene chip for diagnosing SARS coronary virus - Google Patents

Gene chip for diagnosing SARS coronary virus Download PDF

Info

Publication number
CN1285736C
CN1285736C CNB2004100220900A CN200410022090A CN1285736C CN 1285736 C CN1285736 C CN 1285736C CN B2004100220900 A CNB2004100220900 A CN B2004100220900A CN 200410022090 A CN200410022090 A CN 200410022090A CN 1285736 C CN1285736 C CN 1285736C
Authority
CN
China
Prior art keywords
primer
sequence
virus
gene chip
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB2004100220900A
Other languages
Chinese (zh)
Other versions
CN1597984A (en
Inventor
谭德勇
赖建华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yunnan University YNU
Original Assignee
Yunnan University YNU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yunnan University YNU filed Critical Yunnan University YNU
Priority to CNB2004100220900A priority Critical patent/CN1285736C/en
Publication of CN1597984A publication Critical patent/CN1597984A/en
Application granted granted Critical
Publication of CN1285736C publication Critical patent/CN1285736C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a gene chip for diagnosing SARS coronary virus, which belongs to the biotechnology field. The chip of the present invention uses 12 determined specific SARS coronary virus cDNA fragments as detection sites; 12 pairs of PCR primers are used for preparing probe DNA. In addition, 12 pairs of PCR primers of sequences positioned at the outer sides of the probe sites are used as detection primers; specimen cDNA to be detected is hybridized with the chip after being PCR-amplified and marked; whether SARS coronary virus exists in the specimen or not is judged according to a hybridization signal. The probe prepared primers and the detection primers used by the chip can also be singly used as PCR detection primers. The chip can also be used for detecting SARS coronary virus in foods, environment and quarantine. The present invention has the following advantages of decreasing missed detection caused by individual difference, having high detection sensitivity and specificity, eliminating systemic error, controlling detection system pollution, etc. The technology can also be used for detecting SARS coronary virus in foods, environment and quarantine.

Description

A kind of sars coronavirus diagnostic gene chip
Technical field:
The present invention relates to a kind of sars coronavirus diagnostic gene chip, belong to biological technical field.
Background technology:
Severe acute respiratory syndrome is that serious respiratory tract infection syndromes (SARS) is the very strong communicable disease of a kind of infectivity, short 3 wheat harvesting periods of the 2-5 month in 2003, in worldwide, be widely current, cause nearly 8000 people's infection morbidities, cause people's death more than 600, serious threat human health and life security cause the great attention of the World Health Organization and national governments.
Effectively detecting the related diseases original is the basis of effectively controlling this disease popularity.Because this disease pathogen thing is new pathogen, can't detect at the beginning of popular, can only judge according to clinical symptom, cause a lot of virus carriers to flow and infect everywhere and give other people.The patient symptom that has simultaneously is similar to SARS, and also under a cloud is SARS and be isolated in real SARS and infect SARS.Emergent subsequently some immune diagnostic methods that produce and PCR method cause detection accuracy low all because of the restriction of technology itself.Therefore a kind of foundation of effective detection method seems important especially.Biochip technology is a kind of emerging biotechnology, and it has wide practical use in each field of biology, and clinical diagnosis is one of its Application Areas, utilizes the gene chip diagnosis SARS virus can remedy the deficiency of existing diagnostic method.
The pathogen of SARS virus has been measured its nucleotide sequence through the scientist's of the several countries in the world effort in the time about 2 months, find full length sequence 29.4Kb, with the nucleic acid sequence homology of existing coronavirus less (＜10%).According to the nucleotide sequence of having announced, development diagnostic gene chip becomes possibility.
Summary of the invention:
Chip of the present invention with the special cDNA fragment of 12 sars coronavirus as probe, and designed the PCR primer of chip probe manufacturing and used used detection primer sequence (seeing sequence table for details) with chip, making gene chip by prior art (inventor's application, application number 021280681,02128069X) uses with detecting.The used probe of this chip prepares primer and the detection primer also can be used for PCR detection primer separately; This chip also can be used for food, the detection of the sars coronavirus in environment and the quarantine.
Advantage of the present invention:
1. gene chip diagnosis is because the multidigit point can reduce the omission that causes because of individual difference effectively.
2. the detection sensitivity of this product reduces 2-3 the order of magnitude than the detectability of PCR method, can reach 10 2-10 3/ ml viruses molecule.
3. because enough volume space are arranged, various contrasts can be set, eliminate systematic error and control and detection system and pollute.
4. in the testing process,, virus is concentrated, improve detection sensitivity by polyethylene glycol precipitation.
Description of drawings:
Accompanying drawing is a dot chart.I, II, the positive control point of III, IV among the figure, all the other are numbered the detecting position period.
Annex comprises detection sequence (totally 12), the probe primer (totally 24) of 12 detection site on the chip and detects primer (totally 24).Site X during use (X represent 1,2,3.....12) detects sequence, prepares probe with site X probe primer, detects with site X detection primer.
Embodiment:
Enforcement of the present invention is undertaken by following program:
1. utilize internet web search sars coronavirus nucleotide sequence, and utilize the online tool on the NIH website to detect the nucleotide sequence that is different from other coronavirus in the sars coronavirus genome, according to the SARS virus distinguished sequence that obtains, designed 12 detection site, cDNA obtains the cDNA fragment by amplification standard sars coronavirus;
2. the sars coronavirus cDNA fragment of clone PCR amplification checks order and determines clone's exactness.Obtain 12 clones that sequence is correct altogether, with the dna probe of the corresponding fragment of pcr amplification as chip manufacturing;
3. make chip by following technology:
1). prepare primer (primer sequence see Appendix probe make primer) with probe and carry out pcr amplification, clone the special cDNA of SARS, its product ethanol sedimentation, 1XTE dissolving, and quantitative with electrophoresis method;
2). quantitative PCR product is diluted to 0.3ug/ul with 3 * SSC;
3). on the processed slide glass of L-poly-lysine bag, dot chart is seen accompanying drawing with the point sample instrument point sample, with UV-crosslinked method fixed dna on slide glass.
4. when using, adopt existing method (see number of patent application: 021280681,02128069X), but because different purposes, sampling method is also different with the preparation method.
(1) sampling:
1) clinical sample: the about 5ml of physiological saline with sterilization gargles to patient, is facing upward neck when gargling, and washes the upper respiratory tract about 10 seconds, and the collutory that spues is with 50ml centrifuge tube collection collutory;
2) vegetables and fruit: wash the surface of detected sample with the about 5ml of physiological saline, the solution that washes is collected in the centrifuge tube of 50ml;
3) other foods with about 5ml physiological saline and the about 1-2 gram of sample to be checked, stir together.10000g is centrifugal, gets supernatant liquor in the 50ml centrifuge tube.
(2). sample preparation: the above-mentioned solution that contains virus adds 13% polyoxyethylene glycol 8000 of the physiological saline configuration of isopyknic 1.6mol/L, mixing, centrifugal 10 minutes of 10000g removes supernatant, and precipitation adds 100ul physiological saline suspended sediment;
(3) carry out pcr amplification with conventional Trizol reagent isolation of RNA, and with a conventional one step RT-PCR method, detection primer see Appendix (detection primer).
(4) detect patent 021280681 by existing chip, the 02128069X method detects the existence of nucleic acid.
5. by above-mentioned trace routine, standard samples is tested 100% detect reliably.
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 1
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
gccatcattt tggcatcttt ctctgcttct acaagtgcct ttattgacac tataaagagt 60
cttgattaca agtctttcaa aaccattgtt gagtcctgcg gtaactataa agttaecaag 120
ggaaagcccg taaaaggtgc ttggaacatt ggacaacaga gatcagtttt aacaccactg 180
tgtggttttc cctcacaggc tgctggtgtt atcagatcaa tttttgcgcg cacacttgat 240
gcagcaaacc 250
<212>Type：DNA
<211>Length：250
SequenceName：sequence 1
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 1 probe upstream primer
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cattgttgag tcctgcggta 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 25
SequenceDescription：
Organization Applicant
-------------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 1 probe downstream primer
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>Organis mName：virus
<400>PreSequenceString：
taacaccagc agcctgtgag 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 26
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 1
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
gccatcattt tggcatcttt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 1
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 1
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ggtttgctgc atcaagtgtg 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 2
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 2
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
gcaaagttgc gatcactcaa cttaggtgaa gtcttcatcg ctcaaagcaa gggactttac 60
cgtcagtgta tacgtggcaa ggagcagctg caactactca tgcctcttaa ggcaccaaaa 120
gaagtaacct ttcttgaagg tgattcacat gacacagtac ttacctctga ggaggttgtt 180
ctcaagaacg gtgaactcga agcactcgag acgcccgttg atagcttcac aaatggagct 240
atcgttggca c 251
<212>Type：DNA
<211>Length：251
SequenceName：sequence 2
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 2 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tgtatacgtg gcaaggagca 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 27
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 2 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tctcgagtgc ttcgagttca 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 28
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 2
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
gcaaagttgc gatcactcaa 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 3
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 2
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
gtgccaacga tagctccatt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 4
SequenceDescription：
Organization Applicart
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 3
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
taccctccaa aaaggctggt ggcactactg agatgctctc aagagctttg aagaaagtgc 60
cagttgatga gtatataacc acgtaccctg gacaaggatg tgctggttat acacttgagg 120
aagctaagac tgctcttaag aaatgcaaat ctgcatttta tgtactacct tcagaagcac 180
ctaatgctaa ggaagagatt ctaggaactg tatcctggaa tttgagagaa atgcttgctc 240
atgctga 247
<212>Type：DNA
<211>Length：247
SequenceName：sequence 3
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 3 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
aaccacgtac cctggacaag 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 29
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 3 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
agcattaggt gcttctgaag gt 22
<212>Type：DNA
<211>Length：22
SequenceName：primer 30
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 3
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
taccctccaa aaaggctggt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 5
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 3
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tcagcatgag caagcatttc 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 6
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 4
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tgctcgtgat ttgtcactcc agtttaaaag accaatcaac cctactgacc agtcatcgta 60
tattgttgat agtgttgctg tgaaaaatgg cgcgcttcac ctctactttg acaaggctgg 120
tcaaaagacc tatgagagac atccgctctc ccattttgtc aatttagaca atttgagagc 180
taacaacact aaaggttcac tgcctattaa tgtcatagtt tttgatggca agtccaaatg 240
cgacgagtct 250
<212>Type：DNA
<211>Length：250
SequenceName：sequence 4
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 4 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ccaatcaacc ctactgacca 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 31
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 4 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ggagagcgga tgtctctcat 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 32
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 4
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismMame：virus
<400>PreSequenceString：
tgctcgtgat ttgtcactcc 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 7
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 4
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
agactcgtcg catttggact 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 8
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 5
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tggttttgac gcatggttta gccagcgtgg tggttcatac aaaaatgaca aaagctgccc 60
tgtagtagct gctatcatta caagagagat tggtttcata gtgcctggct taccgggtac 120
tgtgctgaga gcaatcaatg gtgacttctt gcattttcta cctcgtgttt ttagtgctgt 180
tggcaacatt tgctacacac cttccaaact cattgagtat agtgattttg ctacctctgc 240
ttgcgttct 249
<212>Type：DNA
<211>Length：249
SequenceName：sequence 5
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 5 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
aatgacaaaa gctgccctgt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 33
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 5 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ttgccaacag cactaaaaac a 21
<212>Type：DNA
<211>Length：21
SequenceName：primer 34
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 5
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tggttttgac gcatggttta 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 9
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 5
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
agaacgcaag cagaggtagc 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 10
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 6
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ggctgcaggt acagacacaa ccataacatt aaatgttttg gcatggctgt atgctgctgt 60
tatcaatggt gataggtggt ttcttaatag attcaccact actttgaatg actttaacct 120
tgtggcaatg aagtacaact atgaaccttt gacacaagat catgttgaca tattgggacc 180
tctttctgct caaacnggaa ttgccgtctt agatatgtgt gctgctttga aagagctgct 240
gcagaatggt 250
<212>Type：DNA
<211>Length：250
SequenceName：sequence 6
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 6 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
catggctgta tgctgctgtt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 35
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 6 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
gagcagaaag aggtcccaat 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 36
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 6
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ggctgcaggt acagacacaa 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 11
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 6
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
accattctgc agcagctctt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 12
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 7
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ttgttgatgc ggatagcaag attgttcaac ttagtgaaat taacatggac aattcaccaa 60
atttggcttg gcctcttatt gttacagctc taagagccaa ctcagctgtt aaactacaga 120
ataatgaact gagtccagta gcactacgac agatgtcctg tgcggctggt accacacaaa 180
cagcttgtac tgatgacaat gcacttgcct actataacaa ttcgaaggga ggtaggtttg 240
tgctggcatt 250
<212>Type：DNA
<211>Length：250
SequenceName：sequence 7
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 7 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tttggcttgg cctcttattg 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 37
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 7 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
aggcaagtgc attgtcatca 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 38
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 7
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ttgttgatgc ggatagcaag 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 13
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 7
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
aatgccagca caaacctacc 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 14
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 8
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cccaggttgt gatgtcactg atgtgacaca actgtatcta ggaggtatga gctattattg 60
caagtcacat aagcctccca ttagttttcc attatgtgct aatggtcagg tttttggttt 120
atacaaaaac acatgtgtag gcagtgacaa tgtcactgac ttcaatgcga tagcaacatg 180
tgattggact aatgctggcg attacatact tgccaacact tgtactgaga gactcaagct 240
tttcgcagc 249
<212>Type：DNA
<211>Length：249
SequenceName：sequence 8
SeauenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 8 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
aagcctccca ttagttttcc a 21
<212>Type：DNA
<211>Length：21
SequenceName：primer 39
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 8 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
aatcgccagc attagtccaa 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 40
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 8
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
-------
<213>OrganismName：virus
<400>PreSequenceString：
cccaggttgt gatgtcactg 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 15
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 8
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
gctgcgaaaa gcttgagtct 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 16
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 9
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cagacaatgg ttgccaactg gcacactaac ttgtcgattc agatcttaat gacttcgtct 60
ccgacgcaga ttctacttta attggagact gtgcaacagt acatacggct aataaatggg 120
accttattat tagcgatatg tatgacccta ggaccaaaca tgtgacaaaa gagaatgact 180
ctaaagaagg gtttttcact tatctgtgtg gatttataaa gcaaaaacta gccctgggtg 240
gtt 243
<212>Type：DNA
<211>Length：243
SequenceName：sequence 9
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：dytan@ynu.edu.en
EmailAddress：
＜110〉OrganizationName: Yunnan University
Application Project
--------------------
＜120〉Title: site 9 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cgtctccgac gcagattcta 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 41
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 9 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
---------
<213>OrganismName：virus
<400>PreSequenceString：
cttttgtcac atgtttggtc ct 22
<212>Type：DNA
<211>Length：22
SequenceName：primer 42
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 9
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cagacaatgg ttgccaactg 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 17
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 9
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
aaccacccag ggctagtttt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 18
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 10
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cttgctcttt tgggggtgta agtgtaatta cacctggaac aaatgcttca tctgaagttg 60
ctgttctata tcaagatgtt aactgcactg atgtttctac agcaattcat gcagatcaac 120
tcacaccagc ttggcgcata tattctactg gaaacaatgt attccagact caagcaggct 180
gtcttatagg agctgagcat gtcgacactt cttatgagtg cgacattcct attggagctg 240
gcatttgtg 249
<212>Type：DNA
<211>Length：249
SequenceName：sequence 10
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 10 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cacctggaac aaatgcttca t 21
<212>Type：DNA
<211>Length：21
SequenceName：primer 43
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 10 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
agcctgcttg agtctggaat 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 44
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 10
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cttgctcttt tgggggtgta 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 19
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 10
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cacaaatgcc agctccaata 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 20
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: sequence is detected in site 11
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<215>OrganismName：virus
<400>PreSequenceString：
accgctcatg gaaagtgaac ttgtcattgg tgctgtgatc attcgtggtc acttgcgaat 60
ggccggacac tccctagggc gctgtgacat taaggacctg ccaaaagaga tcactgtggc 120
tacatcacga acgctttctt attacaaatt aggagcgtcg cagcgtgtag gcactgattc 180
aggttttgct gcatacaacc gctaccgtat tggaaactat aaattaaata cagaccacgc 240
cggtag 246
<212>Type：DNA
<211>Length：246
SequenceName：sequence 11
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 11 probe upstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ttggtgctgt gatcattcgt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 45
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project --------------------
＜120〉Title: site 11 probe downstream primers
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
cctgaatcag tgcctacacg 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 46
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: upstream primer is detected in site 11
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
accgctcatg gaaagtgaac 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 21
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: downstream primer is detected in site 11
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ctaccggcgt ggtctgtatt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 22
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 12 detects sequence
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tcagccccag atggtacttc tattacctag gaactggccc agaagcttca cttccctacg 60
gcgctnacaa agaaggcatc gtatgggttg caactgaggg agccttgaat acacccaaag 120
accacattgg cacccgcaat cctaataaca atgctgccac cgtgctacaa cttcctcaag 180
gaacaacatt gcctaaaagg cttctacgca gagggaagca gaggcggcag tcaagcctct 240
tctcgct 247
<212>Type：DNA
<211>Length：247
SequenceName：sequence 12
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 12 probe upstream primer
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-l0
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
agaaggcatc gtatgggttg 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 47
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 12 probe downstream primer
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
ccctctgcgt agaagccttt 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 48
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress:dytan@ynu.edu.cn＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 12 detects upstream primer
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
tcagccccag atggtacttc 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 23
SequenceDescription：
Organization Applicant
----------------------
Street: No. 2, Green Lake North Road
City: Kunming
State: Yunnan Province
Country: China
PostalCode：650091
PhoneNumber：0871-5032061
FaxNumber：
EmailAddress：dytan@ynu.edu.cn
＜110〉OrganizationName: Yunnan University
Application Project
-------------------
＜120〉Title: site 12 detects downstream primer
＜130〉AppFileReference: a kind of sars coronavirus diagnostic gene chip
<140>CurrentAppNumber：
<141>CurrentFilingDate：2004-03-10
Sequence
--------
<213>OrganismName：virus
<400>PreSequenceString：
agcgagaaga ggcttgactg 20
<212>Type：DNA
<211>Length：20
SequenceName：primer 24
SequenceDescription：

Claims (1)

1, a kind of sars coronavirus diagnostic gene chip, it is characterized in that this chip determined that the special cDNA fragment of a sequence 1-12 sars coronavirus is as detection site, utilize PCR primer 25 and 26, primer 27 and 28, primer 29 and 30, primer 31 and 32, primer 33 and 34, primer 35 and 36, primer 37 and 38, primer 39 and 40, primer 41 and 42, primer 43 and 44, primer 45 and 46 and primer 47 and 48 the preparation dna probes, utilize in addition and be positioned at the PCR primer 1 and 2 that probe site checks order and is listed as outward, primer 3 and 4, primer 5 and 6, primer 7 and 8, primer 9 and 10, primer 11 and 12, primer 13 and 14, primer 15 and 16, primer 17 and 18, primer 19 and 20, primer 21 and 22 and primer 23 and 24 as detecting primers, by pcr amplification sample cDNA to be detected, behind the mark with chip hybridization, according to whether sars coronavirus is arranged in the hybridization signal judgement sample.
CNB2004100220900A 2004-03-18 2004-03-18 Gene chip for diagnosing SARS coronary virus Expired - Fee Related CN1285736C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNB2004100220900A CN1285736C (en) 2004-03-18 2004-03-18 Gene chip for diagnosing SARS coronary virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNB2004100220900A CN1285736C (en) 2004-03-18 2004-03-18 Gene chip for diagnosing SARS coronary virus

Publications (2)

Publication Number Publication Date
CN1597984A CN1597984A (en) 2005-03-23
CN1285736C true CN1285736C (en) 2006-11-22

Family

ID=34663382

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB2004100220900A Expired - Fee Related CN1285736C (en) 2004-03-18 2004-03-18 Gene chip for diagnosing SARS coronary virus

Country Status (1)

Country Link
CN (1) CN1285736C (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111808989A (en) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 Coronavirus/influenza virus/rhinovirus nucleic acid combined detection kit and use method thereof

Also Published As

Publication number Publication date
CN1597984A (en) 2005-03-23

Similar Documents

Publication Publication Date Title
CN1687454A (en) Method for sceening viral nucleic acid of blood through isothermal amplification based on loop mediated technique
CN1948503A (en) Detection and parting method of human papillomavirus and reagent box
CN101058833A (en) Primer system and method for detecting and analyzing avian influenza virus
CN1156587C (en) Chinese dendrobe plant and its medicinal materials DNA molecular diagnosis method
CN1558954A (en) Genotyping kit for diagnosis of human papilloma virus infection
CN1497049A (en) Androgen receptor compound-associated protein
CN100345980C (en) PCR primer set for detecting hepatitis b virus, method for detecting hepatitis b using the primer set, and hepatitis b virus detection kit including the primer set
CN1646705A (en) Amplification-hybridisation method for detecting and typing human papillomavirus
CN1877328A (en) Method for detecting aquatic animal pathogenic bacteria by using 23S ribosome gene probe array
CN1748028A (en) Signal amplification method for detecting mutant gene
CN1616676A (en) Stable hybrid
CN1285736C (en) Gene chip for diagnosing SARS coronary virus
CN1216902C (en) SARS virus specific protein and clinical detection method and kit
CN1566131A (en) Small interference RNA molecule SiRNA capable of attacking human hepatitis B virus and application thereof
CN1276094C (en) DNA chip for diagnosing Mediterranean anemia and its prepn
CN1173989C (en) DNA sequence of dendrobium and method for discriminating its veriety and judging if it is true or false
CN1186457C (en) Homogeneous genetic matrix
CN1606622A (en) Method for detecting and quantifying hemolysin-producing bacteria by comprehensively detecting and quantifying thermostable direct hemolysin-associated gene (tdh) associated hemolysin gene) of food poisoning bacteria
CN1526111A (en) Polymorphic marker that can be used to assess the efficacy of interferon therapy
CN1303218C (en) Method of screening peroxisome proliferator-activated receptor antagon and agonist
CN1877327A (en) Method for detecting aquatic animal pathogenic bacteria by using ribosome interoperonic region probe
CN101037690A (en) BRCA1 gene variant having new single nucleotide polymorphism and encoded protein variant
CN1661372A (en) Immunization microsphere in use for detecting SARS antibody, preparation method and application
CN101052720A (en) Method of detecting H5 or H7 avian influenza virus
CN1966726A (en) Method for assaying REG IV mRNA

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C19 Lapse of patent right due to non-payment of the annual fee
CF01 Termination of patent right due to non-payment of annual fee