CN1278597C - Method for preparing tremella mesenterica cultivating seed - Google Patents
Method for preparing tremella mesenterica cultivating seed Download PDFInfo
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Abstract
The present invention relates to a method for preparing cultivated seed of golden fungus, which is characterized in that the method of the present invention comprises the following steps: a solid culture step and a liquid culture step of the stereum hirsutum of the first ingredient of the cultivated seed of the golden fungus are carried out through the culture of the bacterium seed, a test tube seed culture step, a liquid seed culture step, a rocking bed culture step and a fermentation tank culture step of toruloid spores of the second ingredient of the cultivated seed of the golden fungus are carried out; the first ingredient and the second ingredient of the cultivated seed of the golden fungus are mixed; a linden culture process and a bagging material process or a bottle culture process are carried out. Because the golden fungus bacterium and the associated pilus stereum in the culture of the golden fungus are respectively cultivated, the golden fungus bacterium and the associated pilus stereum can be fully and evenly mixed, the golden fungus and the toruloid spores can fully germinate, and thus, the condition of growing a great amount of the entity of the pilus stereum on the linden, which frequently occurs in the culture of the golden fungus can not occur by using the bacterium seed of the present invention. When the bacterium seed of the present invention is used for cultivating the golden fungus on the linden, the mushroom growing rata can achieve more than 95%, and the yield can be increased by 15 to 20%; when the liquid bacterium seed of the golden fungus liquid of the present invention is used, the golden fungus bacterium and the associated bacterium are evenly mixed, the golden fungus bacterium can quickly grow, the filler absorption is quick, the pollution rate is low, the mushroom growing rata can achieve 100%, the color changing speed is quick, and the growth period is short.
Description
Technical field
The present invention relates to edible fungus culture technique field, the preparation method of the golden ear cultivated species of especially a kind of macro fungi that concomitance bacterium arranged.
Background technology
Gold ear (Tremella aurantialba Bandoni et Zang) is the food and medicament dual-purpose fungi of Basidiomycetes Tremellales Tremellaceae Tremella, mainly is distributed in China southwest, and golden ear artificial cultivation successfully (comprises Duan Mu, cultivating in bag).
Because golden ear is relatively poor to lignin and the cellulosic ability of utilization, need rely under field conditions (factors) and (willd:Fr.) S.F.Gray of the hair Boreostereum vibrans (Stereum hirsutum) of its association) nutrition is provided, could grow.Since the tremella silk a little less than, hair Boreostereum vibrans growth often appears rapidly and the long situation of tremella causes the low yield of ear rate low thus, though also there is the trial that improves golden ear cultivated species preparation method all not achieve success in existing cultivated species production of hybrid seeds process.
Summary of the invention
The objective of the invention is to overcome the problem that existing golden ear cultivated species exists, a kind of preparation method who uses solid-liquid and liquid-liquid technology golden ear cultivated species that make, that golden ear output is high at segment wood cultivated and cultivating in bag, bottle cultivation respectively is provided.
The present invention makes cultivated species by the way of the production of hybrid seeds respectively respectively with Jin Er and concomitance bacterium hair Boreostereum vibrans, by hybrid technology solve in the original production of hybrid seeds since the tremella growth uneven and not vigorous cause go out the problem that the ear rate is low and yield poorly.
Gold ear concomitance bacterium hair Boreostereum vibrans (Stereum hirsutum) is S.F.Gray (willd:Fr.)) be to separate the bacterial strain that obtains by Protoplast Technique, numbering yaasm-08; Tremella is yeast shape (yeast-like) spore that obtains by spore separation, numbering yaasm-17.
The preparation method of golden ear cultivated species of the present invention is: by weight percentage raw materials used:
1) culture of strains:
The solid culture of one of a, golden ear cultivated species component hair Boreostereum vibrans: 20～28 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 5～10 days are seeded on the solid culture medium cultivate, cultivated 30～45 days for 20～28 ℃.Test tube kind medium is PDA, and solid culture medium is an oak wood chip 70%, wheat bran 20%, analysis for soybean powder 5%, corn flour 5%;
The liquid culture of one of b, golden ear cultivated species component hair Boreostereum vibrans: 20～28 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 5～10 days are seeded in the liquid seed culture medium, cultivate after 5～10 days for 20～28 ℃, 5～10% the ratio of cultivating kind in cultivating base unit weight is connected in shaking table or the fermentation tank culture medium, the shaking table condition of culture is 20～28 ℃, 100～200rpm, incubation time 5～7 days, the fermentation tank culture condition stirs 20～28 ℃ and cultivated 5～8 days for ventilation 0.5～2L/min, 100～300rpm; Test tube kind medium is PDA, and liquid seed culture medium is 5～10% analysis for soybean powder, 5～10% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, water surplus; The medium of shaking table or fermentation tank culture is 10～15% analysis for soybean powder, 10～15% corn flour, oak sawdust 5～10%, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, water surplus;
The test tube kind of two gold medal ear yeast shape spores of c, golden ear cultivated species component is cultivated: golden ear yeast shape spore inoculating is being improved on the PDA medium slant of going, cultivated 7～10 days for 20～28 ℃, improved PDA medium is that 10% potato is boiled liquid and adds 0.05～0.2% dusty yeast or cream, 0.05～0.2% peptone, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, 2% glucose or sucrose;
The liquid seeds of two gold medal ear yeast shape spores of d, golden ear cultivated species component is cultivated: add water surplus in 5～10% analysis for soybean powder, 5～10% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert the test tube kind, 20～28 ℃ of 100～300rpm shaking tables were cultivated 3～10 days;
Two shaking table of e, golden ear cultivated species component is cultivated: add water surplus in 5～15% analysis for soybean powder, 5～15% corn flour, 5～10% oak sawdusts, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, stir and be divided in the triangular flask 121 ℃ of sterilizations 30～60 minutes, be cooled to 35 ℃, insert to cultivate 5～10% previous step gained seed of base unit weight, 20～28 ℃, 100～300rpm shaking table were cultivated 5～8 days;
Two fermentation tank culture of f, golden ear cultivated species component: in 5～15% analysis for soybean powder, 5～15% corn flour, 5～10% oak sawdusts, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, add water surplus, stir and be contained in the fermentation tank, sterilized 30～60 minutes for 121 ℃, be cooled to 35 ℃, insert to cultivate 5～10% previous step gained seed of base unit weight, ventilation 0.5～2L/min, 100～300rpm stir 20～28 ℃ and cultivated 5～8 days;
2) mixing of golden ear cultivated species component one and component two:
Two shaking table of the solid culture of one of a, component and component or fermentation tank culture thing mix in 2: 1 or 1: 2 ratio, and room temperature condition was placed 5～10 days down, the golden ear solid-liquid of one of De Jiner cultivated species bacterial classification, this bacterial classification can inoculate Duan Mu, bottle and bag;
Two shaking table or fermentation tank culture thing of the liquid culture of one of b, component and component mix in 2: 1～1: 2 ratio, place under the room temperature 1～3 day, and two tremella fluid fluid strains of De Jiner cultivated species can be inoculated bag or bottle;
3) cultivation
A, segment wood cultivated: choose suitable Duan Mu by existing segment wood cultivated method punching, inoculate golden ear solid-liquid bacterial classification, management method is with general solid spawn;
B, cultivating in bag or bottle cultivation: make cultivate material by general golden ear cultivating in bag or bottle cultivation, every bottle or bag inoculation tremella fluid fluid strain 3～5%, the inoculation back is 20～28 ℃ of cultivations, mycelia was covered with bottle or bag in 40～48 days, press natural temperature differential and cultivated 18～22 days this moment, moves into the fruiting real estate management and manage when the golden ears or side handles of a utensil entity seen in bottle or the bag more than the 2cm.
The present invention has following good effect compared with the prior art:
1) because tremella and concomitance bacterium hair Boreostereum vibrans in the golden ear cultivation are cultivated respectively, tremella and concomitance bacterium can mix full and uniformly, and before inoculation, placed 5～10 days, make the yeast shape spore of golden ear fully to sprout, the Duan Mu that uses bacterial classification of the present invention can not go out normal appearance in the cultivation of cash ear like this goes up the situation of a large amount of hair Boreostereum vibrans fruit body of growth, cultivating rate is more than 95% when using the segment wood cultivated golden ear of bacterial classification of the present invention, and output increases by 15～20%;
2) use tremella fluid fluid strain of the present invention, tremella and concomitance bacterium mix, and send out when golden ear is planted or bag planted to bottle that bacterium is fast, material feeding is fast, pollution rate is low, and less than 5%, cultivating rate reaches 100%, annesl is fast, and growth cycle shortens (5～10 days), and output increases by 15～20%.
Embodiment
The used percentage of embodiment 1:(is weight percentage)
1, culture of strains
1) the test tube kind of two gold medal ear yeast shape spores of golden ear cultivated species component is cultivated: golden ear yeast shape spore inoculating on improved PDA medium slant 20 ℃ cultivated 10 days, improving the PDA medium and be 10% potato boils liquid and adds 0.05% dusty yeast, 0.05% peptone, 0.05% dipotassium hydrogen phosphate (K
2HPO
4), 0.05% magnesium sulfate (MgSO
4), 2% glucose;
2) liquid seeds of two gold medal ear yeast shape spores of golden ear cultivated species component is cultivated: in 5% analysis for soybean powder, 5% corn flour, 0.05% dipotassium hydrogen phosphate, add 100% water in 0.05% magnesium sulfate, stirring is divided in the triangular flask, sterilizes 45 minutes, and is cooled to 35 ℃ for 121 ℃, insert the test tube kind, 20 ℃ of 100rpm shaking tables were cultivated 10 days;
3) shaking table of two gold medal ear yeast shape spores of golden ear cultivated species component is cultivated: add water surplus in 5% analysis for soybean powder, 5% corn flour, 5% oak sawdust, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, stir and be divided in the triangular flask, sterilized 45 minutes for 121 ℃, be cooled to 35 ℃, insert to cultivate 5～10% previous step gained seed of base unit weight, 20 ℃ of 100rpm shaking tables were cultivated 8 days;
4) fermentation tank culture of two gold medal ear yeast shape spores of golden ear cultivated species component: in 5% analysis for soybean powder, 5% corn flour, 5% oak sawdust, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, add water surplus, stir and be contained in the fermentation tank, sterilized 45 minutes for 121 ℃, be cooled to 35 ℃, insert to cultivate 5～10% previous step gained seed of base unit weight, ventilation 0.5L/min, 100rpm stir 20 ℃ and cultivated 8 days;
5) solid culture of one of golden ear cultivated species component hair Boreostereum vibrans: 20 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 7 days are seeded on the solid culture medium cultivate, cultivated 40 days for 20 ℃.Test tube kind medium is PDA; Solid culture medium is an oak wood chip 70%, wheat bran 20%, analysis for soybean powder 5%, corn flour 5%.
6) liquid culture of one of golden ear cultivated species component hair Boreostereum vibrans: 20 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 10 days are seeded in the liquid seed culture medium, cultivating 10% the ratio ratio that will cultivate after 7 days kind in the cultivation base unit weight for 20 ℃ is connected in shaking table or the fermentation tank culture medium, the shaking table condition of culture is 20 ℃, 100rpm, incubation time 7 days, the fermentation tank culture condition stirs 20 ℃ and cultivated 8 days for ventilation 0.5L/min, 100rpm.Test tube kind medium is PDA; Liquid seed culture medium is 5% analysis for soybean powder, 5% corn flour, and 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate adds water surplus; The medium of shaking table or fermentation tank culture is 10% analysis for soybean powder, 10% corn flour, and oak sawdust 10%, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate adds water surplus.
2, the mixing of golden ear cultivated species component one and component two
1) segment wood cultivated kind: two shaking table or fermentation tank culture thing of the solid culture of one of component and component mix in 2: 1 ratios, and room temperature condition is placed down can inoculate Duan Mu in 5 days;
2) cultivating in bag kind or bottle cultivated species: two shaking table or fermentation tank culture thing of the liquid culture of one of component and component mix in 2: 1 ratio, under the room temperature placement can inoculate after 1 day bag or bottle.
3, cultivation
1) segment wood cultivated: choose suitable Duan Mu by existing segment wood cultivated method punching, inoculate golden ear solid-liquid bacterial classification of the present invention, management method is with general solid spawn.
2) cultivating in bag kind: make cultivate material by general golden ear cultivating in bag kind, every bag of inoculation tremella fluid fluid strain 3% of the present invention, the inoculation back is 20 ℃ of cultivations, mycelia was covered with bottle or bag in 48 days, press natural temperature differential and cultivated 18 days this moment, moves into the fruiting real estate management and manage when the golden ears or side handles of a utensil entity seen in bottle or the bag more than the 2cm.
Embodiment 2:
1, culture of strains
1) two of golden ear cultivated species component test tube kind is cultivated: golden ear yeast shape spore inoculating on improved PDA medium slant 25 ℃ cultivated 7 days, improving the PDA medium and be 10% potato boils liquid and adds 0.2% yeast extract, 0.2% peptone, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, 2% glucose;
2) two of golden ear cultivated species component liquid seeds is cultivated: in 8% analysis for soybean powder, 8% corn flour, 0.1% dipotassium hydrogen phosphate, add water surplus in 0.1% magnesium sulfate, stirring is divided in the triangular flask, sterilizes 30 minutes, and is cooled to 35 ℃ for 121 ℃, insert the test tube kind, 25 ℃ of 300rpm shaking tables were cultivated 7 days;
3) two of golden ear cultivated species component shaking table is cultivated: add water surplus in 8% analysis for soybean powder, 8% corn flour, 8% oak sawdust, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate, stir and be divided in the triangular flask, sterilized 30 minutes for 121 ℃, be cooled to 35 ℃, insert to cultivate 8% previous step (suddenly) gained (cultivation) seed of base unit weight, 25 ℃ of 300rpm shaking tables cultivations 7 days;
4) two of golden ear cultivated species component fermentation tank culture: in 8% analysis for soybean powder, 8% corn flour, 8% oak sawdust, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate, add water surplus, stir and be contained in sterilization back cooling in the fermentation tank, insert to cultivate 8% previous step gained (cultivation) seed of base unit weight, ventilation 2L/min, 300rpm stir 25 ℃ and cultivated 7 days;
5) solid culture of one of golden ear cultivated species component hair Boreostereum vibrans: 25 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 5 days are seeded on the solid culture medium cultivate, cultivated 35 days for 25 ℃.Test tube kind medium is PDA; Solid culture medium is an oak wood chip 70%, wheat bran 20%, analysis for soybean powder 5%, corn flour 5%.
6) liquid culture of one of golden ear cultivated species component hair Boreostereum vibrans: 25 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 5 days are seeded in the liquid seed culture medium, cultivating 10% the ratio that will cultivate after 7 days kind in the cultivation base unit weight for 25 ℃ is connected in shaking table or the fermentation tank culture medium, the shaking table condition of culture is 25 ℃, 100rpm, incubation time 5 days, the fermentation tank culture condition stirs 25 ℃ and cultivated 5 days for ventilation 2L/min, 100rpm.Test tube kind medium is PDA; Liquid seed culture medium is 8% analysis for soybean powder, 8% corn flour, and 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate adds water surplus; The medium of shaking table or fermentation tank culture is 13% analysis for soybean powder, 13% corn flour, and oak sawdust 7%, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate adds water surplus.
2, the mixing of golden ear cultivated species component one and component two
1) segment wood cultivated kind: two of the solid culture of one of component and component mixed in 1: 1 ratio, and room temperature condition is placed down can inoculate Duan Mu in 8 days;
2) cultivating in bag kind or bottle cultivated species: two of the liquid culture of one of component and component mixed in 1: 1.5 ratio, under the room temperature placement can inoculate in 2 days bag or bottle.
3, cultivation
1) segment wood cultivated: choose suitable Duan Mu by existing segment wood cultivated method punching, inoculate golden ear solid-liquid bacterial classification of the present invention, management method is with general solid spawn.
2) bottle cultivated species: make cultivate material by general golden ear bottle cultivation, every bottle graft kind tremella fluid fluid strain 4% of the present invention, inoculation back are 25 ℃ of cultivations, and mycelia was covered with bottle or bag in 45 days, press natural temperature differential and cultivated 20 days this moment, moves into the fruiting real estate management and manage when the golden ears or side handles of a utensil entity seen in bottle or the bag more than the 2cm.
Embodiment 3:
1, culture of strains
1) two of golden ear cultivated species component test tube kind is cultivated: golden ear yeast shape spore inoculating on improved PDA medium slant 28 ℃ cultivated 7 days, improving the PDA medium and be 10% potato boils liquid and adds 0.2% dusty yeast, 0.2% peptone, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, 2% sucrose;
2) two of golden ear cultivated species component liquid seeds is cultivated: in 10% analysis for soybean powder, 10% corn flour, 0.2% dipotassium hydrogen phosphate, add water surplus in 0.2% magnesium sulfate, stirring is divided in the triangular flask, sterilizes 60 minutes, and is cooled to 35 ℃ for 121 ℃, insert the test tube kind, 28 ℃ of 300rpm shaking tables were cultivated 3 days;
3) two of golden ear cultivated species component shaking table is cultivated: add water surplus in 10% analysis for soybean powder, 10% corn flour, 10% oak sawdust, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, stir and be divided in the triangular flask, sterilized 60 minutes for 121 ℃, be cooled to 35 ℃, insert to cultivate 10% previous step gained (cultivations) seed of base unit weight, 28 ℃ of 300rpm shaking tables cultivations 5 days;
4) two of golden ear cultivated species component fermentation tank culture: in 15% analysis for soybean powder, 15% corn flour, 10% oak sawdust, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate, add water surplus, stir and be contained in the fermentation tank, sterilized 60 minutes for 121 ℃, be cooled to 35 ℃, insert to cultivate 10% previous step gained (cultivation) seed of base unit weight, ventilation 2L/min, 300rpm stir 28 ℃ and cultivated 5 days;
5) solid culture of one of golden ear cultivated species component hair Boreostereum vibrans: 28 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 7 days are seeded on the solid culture medium cultivate, cultivated 40 days for 28 ℃.Test tube kind medium is PDA; Solid culture medium is an oak wood chip 70%, wheat bran 20%, analysis for soybean powder 5%, corn flour 5%.
6) liquid culture of one of golden ear cultivated species component hair Boreostereum vibrans: 28 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 7 days are seeded in the liquid seed culture medium, cultivating 10% the ratio that will cultivate after 7 days kind in the cultivation base unit weight for 28 ℃ is connected in shaking table or the fermentation tank culture medium, the shaking table condition of culture is 28 ℃, 100rpm, incubation time 5 days, the fermentation tank culture condition stirs 28 ℃ and cultivated 7 days for ventilation 2L/min, 300rpm.Test tube kind medium is PDA; Liquid seed culture medium is 10% analysis for soybean powder, 10% corn flour, and 0.15% dipotassium hydrogen phosphate, 0.1% magnesium sulfate adds water surplus; The medium of shaking table or fermentation tank culture is 15% analysis for soybean powder, 15% corn flour, and oak sawdust 10%, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate adds water surplus.
2, the mixing of golden ear cultivated species component one and component two
1) segment wood cultivated kind: two of the solid culture of one of component and component mixed in 1: 2 ratio, and room temperature condition is placed down can inoculate Duan Mu in 10 days;
2) cultivating in bag kind or bottle cultivated species: two of the liquid culture of one of component and component mixed in 1: 2 ratio, under the room temperature placement can inoculate in 3 days bag or bottle.
3, cultivation
1) segment wood cultivated: choose suitable Duan Mu by existing segment wood cultivated method punching, inoculate golden ear solid-liquid bacterial classification of the present invention, management method is with general solid spawn.
2) cultivating in bag kind: make cultivate material by general golden ear cultivating in bag kind, every bottle or bag inoculation tremella fluid fluid strain 5% of the present invention, the inoculation back is 28 ℃ of cultivations, mycelia was covered with bottle or bag in 40 days, press natural temperature differential and cultivated 22 days this moment, moves into the fruiting real estate management and manage when the golden ears or side handles of a utensil entity seen in bottle or the bag more than the 2cm.
Claims (1)
1, a kind of preparation method of golden ear cultivated species is characterized in that carrying out according to the following steps, and is by weight percentage raw materials used:
1) culture of strains:
The solid culture of one of a, golden ear cultivated species component hair Boreostereum vibrans: 20～28 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 5～10 days are seeded on the solid culture medium cultivate, cultivated 30～45 days for 20～28 ℃.Test tube kind medium is PDA, and solid culture medium is an oak wood chip 70%, wheat bran 20%, analysis for soybean powder 5%, corn flour 5%;
The liquid culture of one of b, golden ear cultivated species component hair Boreostereum vibrans: 20～28 ℃ of hair Boreostereum vibrans test tube kinds of cultivating 5～10 days are seeded in the liquid seed culture medium, cultivate after 5～10 days for 20～28 ℃, 5～10% the ratio of cultivating kind in cultivating base unit weight is connected in shaking table or the fermentation tank culture medium, the shaking table condition of culture is 20～28 ℃, 100～200rpm, incubation time 5～7 days, the fermentation tank culture condition stirs 20～28 ℃ and cultivated 5～8 days for ventilation 0.5～2L/min, 100～300rpm; Test tube kind medium is PDA, and liquid seed culture medium is 5～10% analysis for soybean powder, 5～10% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, water surplus; The medium of shaking table or fermentation tank culture is 10～15% analysis for soybean powder, 10～15% corn flour, oak sawdust 5～10%, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, water surplus;
The test tube kind of two gold medal ear yeast shape spores of c, golden ear cultivated species component is cultivated: with golden ear yeast shape spore inoculating on improved PDA medium slant, cultivated 7～10 days for 20～2 ℃, improved PDA medium is that 10% potato is boiled liquid and adds 0.05～0.2% dusty yeast or cream, 0.05～0.2% peptone, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, 2% glucose or sucrose;
The liquid seeds of two gold medal ear yeast shape spores of d, golden ear cultivated species component is cultivated: add water surplus in 5～10% analysis for soybean powder, 5～10% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert the test tube kind, 20～28 ℃ of 100～300rpm shaking tables were cultivated 3～10 days;
Two shaking table of e, golden ear cultivated species component is cultivated: add water surplus in 5～15% analysis for soybean powder, 5～15% corn flour, 5～10% oak sawdusts, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, stir and be divided in the triangular flask 121 ℃ of sterilizations 30～60 minutes, be cooled to 35 ℃, insert to cultivate 5～10% steps d gained seed of base unit weight, 20～28 ℃, 100～300rpm shaking table were cultivated 5～8 days;
Two fermentation tank culture of f, golden ear cultivated species component: in 5～15% analysis for soybean powder, 5～15% corn flour, 5～10% oak sawdusts, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, add water surplus, stir and be contained in the fermentation tank, sterilized 30～60 minutes for 121 ℃, be cooled to 35 ℃, insert to cultivate 5～10% previous step gained seed of base unit weight, ventilation 0.5～2L/min, 100～300rpm stir 20～28 ℃ and cultivated 5～8 days;
2) mixing of golden ear cultivated species component one and component two:
Two shaking table or fermentation tank culture thing of the solid culture of one of a, component and component mix in 2: 1～1: 2 ratio, and room temperature condition was placed 5～10 days down, the golden ear solid-liquid of one of De Jiner cultivated species bacterial classification, this bacterial classification can inoculate Duan Mu, bottle and bag;
Two shaking table or fermentation tank culture thing of the liquid culture of one of b, component and component mix in 2: 1～1: 2 ratio, place under the room temperature 1～3 day, and two golden fungus liquid bacterial classifications of De Jiner cultivated species can be inoculated bag or bottle;
3) cultivation
A, segment wood cultivated: choose Duan Mu by existing segment wood cultivated method punching, inoculate golden ear solid-liquid bacterial classification, management method is with general solid spawn;
B, cultivating in bag or bottle cultivation: make cultivate material by general golden ear cultivating in bag or bottle cultivation, every bottle or bag inoculation tremella fluid fluid strain 3～5%, the inoculation back is 20～28 ℃ of cultivations, mycelia was covered with bottle or bag in 40～48 days, press natural temperature and cultivated 18～22 days this moment, moves into the fruiting real estate management and manage when bottle or the golden ears or side handles of a utensil entity more than the built-in 2cm of bag.
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| CN106613349A (en) * | 2016-12-15 | 2017-05-10 | 普洱滇洪俊生物科技开发有限公司 | Tremella aurantialba substitute cultivation method |
| CN108440067A (en) * | 2018-05-10 | 2018-08-24 | 习水县龙洋生态食品开发有限公司 | A kind of pholiota nameko culture medium |
| CN108990704A (en) * | 2018-08-17 | 2018-12-14 | 蔡树威 | A kind of production method of golden fungus liquid strain |
| CN111357564A (en) * | 2020-04-01 | 2020-07-03 | 云南菌视界生物科技有限公司 | Tremella aurantialba sporocarp fusion symbiotic culture method |
| CN112205242A (en) * | 2020-11-19 | 2021-01-12 | 中华全国供销合作总社昆明食用菌研究所 | Golden fungus liquid strain inoculating and cultivating method |
| CN114736813A (en) * | 2022-06-14 | 2022-07-12 | 云南菌视界生物科技有限公司 | Method for isolated culture of inner ring stereum hirsutum and application thereof |
-
2004
- 2004-04-21 CN CN 200410022376 patent/CN1278597C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348833B (en) * | 2008-07-16 | 2010-12-01 | 江苏同源堂生物工程有限公司 | ITS sequence of Tremella aurantiablba Bandoni and Stereum hirsutum and method for identifying golden fungus using the same |
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