CN1273970A - Process for extracting beta-carrotin in wolfberry pigment with supercritical CO - Google Patents
Process for extracting beta-carrotin in wolfberry pigment with supercritical CO Download PDFInfo
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- CN1273970A CN1273970A CN00101474A CN00101474A CN1273970A CN 1273970 A CN1273970 A CN 1273970A CN 00101474 A CN00101474 A CN 00101474A CN 00101474 A CN00101474 A CN 00101474A CN 1273970 A CN1273970 A CN 1273970A
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- extraction
- wolfberry pigment
- luobusu
- wolfberry
- matrimony vine
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
A process for extracting beta-carrotin from wolfberry pigment with supercritical CO2 includes superfiltering the raw wolfberry fruit juice with 500-mesh cloth filter and high-molecular (500000) membrane to separate out thick liquid of wolfberry pigment, vacuum freezing to obtain pigment powder, crushing the separted-out wolfberry seeds, proportional mixing, and supercritical CO2 extraction at a certain temp and pressure for 5-6 hrs to obtain beta-carotin. Its advantages include higher output rate and better pharmacological action.
Description
The invention belongs to the biotechnology pharmaceutical field.
The output of β-Hu Luobusu in the world is very little at present, generally is chemical synthesis process, as the sumitomo chemical company of Japan, produces about 1.2 tons per year.Famous Switzerland pharmacy corporation Luo Shi company (ROCHE) also produces a small amount of β-Hu Luobusu, but all-pass cross Subsidiary Company in Japan be given to Japan three altogether companies make various formulation products.The unit of domestic production carotene and quantity are still less, that uses unartificial synthesis method has only Tianjin Lan Tai bio-engineering corporation to bear country's " the Seventh Five-Year Plan " brainstorm subject to extract natural Beta-carotene in Ji Lantai saltern, the Inner Mongol with the bittern culturing bacterium, only 500 kilograms of annual production.Particularly carotenoid physiological function research starts to walk very late to the research of carotenoid in the wolfberry fruit in China.Past only thinks that β-Radix Dauci Sativae is a vitamin a source, has the biological activity of vitamin A.Recent study personnel find that β-Hu Luobusu in that obvious effect is arranged aspect cancer-resisting and the preventing cardiovascular disease, has a wide range of applications.Particularly natural Beta-carotene is better more than the pharmacological action of the carotene of synthetic.How effectively to extract natural Beta-carotene from matrimony vine, be one difficulty of capturing always.
The objective of the invention is at existing β-Hu Luobusu output little, and extract the weak point that adopts chemical synthesis process, propose a kind of wolfberry pigment that is rich in natural Beta-carotene of from the matrimony vine berry, developing, from wolfberry pigment, effectively extract the method for β-Hu Luobusu.
Technical scheme of the present invention is such.At first select for use 500 order filter clothes and 500,000 molecular weight polymeric membranes that Fructus Lycii original juice is carried out pre-treatment---ultrafiltration.Get off the wolfberry pigment in the Fructus Lycii original juice with greater than the insoluble solid particulate trap of 500,000 molecular weight, isolate thick wolfberry pigment liquid, yield is 10-11%.Re-use Vacuum Freezing ﹠ Drying Technology and be lyophilized into pigmented powder, yield is 18-19%, as the raw material of supercritical carbon dioxide extraction β-Hu Luobusu.Then, isolated matrimony vine seed was pulverized 40 mesh sieves during the production Fructus Lycii original juice, be mixed to join in the supercritical carbon dioxide extraction equipment extraction kettle with freeze-drying wolfberry pigment powder by a certain percentage, under certain pressure and temperature condition,, obtain β-Hu Luobusu through 5-6 hour.
The present invention has following effect: 1. the content beta-carotene in the matrimony vine berry is 117.4mg/100g, and about the separable and dry wolfberry pigmented powder 20kg of 1 ton of Fructus Lycii original juice, content beta-carotene is more than 6% in the wolfberry pigment powder.Content beta-carotene in the matrimony vine berry is much bigger than other plant, select isolating wolfberry pigment powder extraction β-Hu Luobusu from Fructus Lycii original juice for use, the one, the extraction yield height, the 2nd, the carotene that is extracted is natural Beta-carotene, and is better than the carotene pharmacological action of synthetic.2. the wolfberry pigment powder uses supercritical co equipment to extract β-Hu Luobusu, selects for use the matrimony vine oil in the matrimony vine seed can improve extraction yield as carrier.An amount of matrimony vine seed is the underlying condition of extraction β-Hu Luobusu from the wolfberry pigment powder as carrier.
Embodiments of the invention are as follows:
At first from fresh matrimony vine berry, produce Fructus Lycii original juice, select for use 500 order filter clothes and 500,000 molecular weight polymeric membranes that Fructus Lycii original juice is carried out pretreatment-super filtering.Get off the wolfberry pigment in the Fructus Lycii original juice with greater than the insoluble solid particulate trap of 500,000 molecular weight, isolate thick wolfberry pigment liquid, yield is 10-11%, re-uses Vacuum Freezing ﹠ Drying Technology and is lyophilized into pigmented powder, and yield is 18-19%.Isolated matrimony vine seed was pulverized 40 mesh sieves during the production Fructus Lycii original juice, mixed with freeze-drying wolfberry pigment powder in proportion, joined in the supercritical carbon dioxide extraction equipment extraction kettle and extracted β-Hu Luobusu.Charge proportion and β-Hu Luobusu extraction yield have following two embodiment:
One, by matrimony vine seed 400g, the ratio of wolfberry pigment powder 1400g (ratio of the two is 1: 3.5) is put into supercritical carbon dioxide extraction equipment extraction kettle, at initial pressure 25MPa, extract under 49 ℃ of conditions of starting temperature, when pressure reaches 27MPa, when temperature is 45 ℃, extract is put into extraction-container I, pressure is 9MPa, starting temperature is 40 ℃, when temperature is 36 ℃, extract is put into extraction-container II, initial pressure is 6MPa, starting temperature is 35 ℃, when pressure reaches 8MPa, when temperature is 30 ℃, whole extraction process is finished, and the time is 6 hours.At this moment extract weight is 69g, and percentage extraction is 17.25%, and content beta-carotene is 64% in the extract.The weight of the β-Hu Luobusu that extracts from the wolfberry pigment powder is 44g, and the yield of β-Hu Luobusu is 51.5%.The wolfberry pigment powder contains β-Hu Luobusu 6.1%, and β-Hu Luobusu 0.61%, 90% β-Hu Luobusu proposes extraction yield 90% in the bits of extraction back.
Two, by matrimony vine seed 380g, the ratio of wolfberry pigment powder 1440g (ratio of the two is 1: 3.74) is put into supercritical carbon dioxide extraction equipment extraction kettle, its pressure, temperature and time condition are with embodiment one, at this moment the extract gross weight is 82, percentage extraction is 21.6%, and the content of β-Hu Luobusu is 56.5% in the extract.The β-Hu Luobusu weight that extracts from wolfberry pigment is 46.1g, and the yield of β-Hu Luobusu is 53.2%.
Extraction equipment among the embodiment adopts the single extraction kettle of HA 120-45-05-bilingual parser formula supercritical carbon dioxide extraction equipment.
Adopt 201-2001 supercritical carbon dioxide extraction equipment in the production.
Claims (3)
1. the method for β-Hu Luobusu in the supercritical carbon dioxide extraction wolfberry pigment is characterized in that:
A. select for use 500 order filter clothes and 500,000 molecular weight polymeric membranes that Fructus Lycii original juice is carried out pretreatment-super filtering, get off the wolfberry pigment in the Fructus Lycii original juice with greater than the insoluble solid particulate trap of 500,000 molecular weight, isolate thick wolfberry pigment liquid, yield 10-11%, re-use Vacuum Freezing ﹠ Drying Technology and be lyophilized into pigmented powder, yield 18-19%;
Isolated matrimony vine seed was pulverized 40 mesh sieves during b. the production Fructus Lycii original juice, be mixed to join in the supercritical carbon dioxide extraction equipment extraction kettle with freeze-drying wolfberry pigment powder by a certain percentage, under certain pressure and temperature condition,, obtain β-Hu Luobusu through 5-6 hour.
2. the method for β-Hu Luobusu in the supercritical carbon dioxide extraction wolfberry pigment according to claim 1, the ratio of wolfberry pigment powder and matrimony vine seed is: wolfberry pigment powder (g): matrimony vine seed (g)=3.5-3.74: 1, because the matrimony vine oil in the matrimony vine seed is as carrier, the yield of β-Hu Luobusu can reach more than 50%, extraction rate reached to 90%.
3. the method for β-Hu Luobusu in the supercritical carbon dioxide extraction wolfberry pigment according to claim 1, it is characterized in that the initial pressure that wolfberry pigment powder and matrimony vine seed join in the extraction kettle is 25MPa, termination pressure is 27MPa, and starting temperature is 49 ℃, and final temperature is 45 ℃; Pressure through extraction-container I is 9MPa, and starting temperature is 40 ℃, and final temperature is 36 ℃, and the initial pressure of process extraction-container II is 6MPa, and termination pressure is 8MPa, and starting temperature is 35 ℃, and final temperature is 30 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00101474A CN1273970A (en) | 2000-01-20 | 2000-01-20 | Process for extracting beta-carrotin in wolfberry pigment with supercritical CO |
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| CN00101474A CN1273970A (en) | 2000-01-20 | 2000-01-20 | Process for extracting beta-carrotin in wolfberry pigment with supercritical CO |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100400133C (en) * | 2005-05-25 | 2008-07-09 | 全亚平 | Supercritical Chinese wolfberry fruit dregs extract and its production method |
| CN100463902C (en) * | 2006-10-31 | 2009-02-25 | 袁晓振 | Natural wolfberry beta carotene and production method |
| CN103130698A (en) * | 2011-12-01 | 2013-06-05 | 周学义 | Method extracting maize yellow from medlar and medlar dregs |
| CN104893355A (en) * | 2015-04-23 | 2015-09-09 | 宁夏大学 | Fructus lycii fat-soluble pigment preparation method |
| CN105113288A (en) * | 2015-09-08 | 2015-12-02 | 湖州宏鑫绸厂 | Method for naturally dyeing fiber by using carotenoid |
| CN105327001A (en) * | 2015-10-28 | 2016-02-17 | 青海尤尼克生物技术有限公司 | Lycium ruthenicum Murr. pigment extract and preparation method thereof |
| CN106317243A (en) * | 2016-08-30 | 2017-01-11 | 宁夏沃福百瑞枸杞产业股份有限公司 | Preparation method of lycium barbarum polysaccharides |
-
2000
- 2000-01-20 CN CN00101474A patent/CN1273970A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100400133C (en) * | 2005-05-25 | 2008-07-09 | 全亚平 | Supercritical Chinese wolfberry fruit dregs extract and its production method |
| CN100463902C (en) * | 2006-10-31 | 2009-02-25 | 袁晓振 | Natural wolfberry beta carotene and production method |
| CN103130698A (en) * | 2011-12-01 | 2013-06-05 | 周学义 | Method extracting maize yellow from medlar and medlar dregs |
| CN104893355A (en) * | 2015-04-23 | 2015-09-09 | 宁夏大学 | Fructus lycii fat-soluble pigment preparation method |
| CN105113288A (en) * | 2015-09-08 | 2015-12-02 | 湖州宏鑫绸厂 | Method for naturally dyeing fiber by using carotenoid |
| CN105113288B (en) * | 2015-09-08 | 2017-12-08 | 重庆欧荣莱汽车配件有限公司 | A kind of method for carrying out natural dyeing to fiber using carotenoid |
| CN105327001A (en) * | 2015-10-28 | 2016-02-17 | 青海尤尼克生物技术有限公司 | Lycium ruthenicum Murr. pigment extract and preparation method thereof |
| CN106317243A (en) * | 2016-08-30 | 2017-01-11 | 宁夏沃福百瑞枸杞产业股份有限公司 | Preparation method of lycium barbarum polysaccharides |
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