CN1270606C - Method for making luminous flower and fluorescene improver - Google Patents
Method for making luminous flower and fluorescene improver Download PDFInfo
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- CN1270606C CN1270606C CN 03118971 CN03118971A CN1270606C CN 1270606 C CN1270606 C CN 1270606C CN 03118971 CN03118971 CN 03118971 CN 03118971 A CN03118971 A CN 03118971A CN 1270606 C CN1270606 C CN 1270606C
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Abstract
The present invention relates to a manufacturing method for noctilucent flowers, particularly to noctilucent orchids, and a fluorescence improver used in manufacture. The manufacturing method of the present invention comprises: a. selecting a flower which has fluorescin genes or is used as an autofluorescence carrier as a material; b. preparing a fluorescence improver; c. coating the fluorescence improver on the flower bud of the flower to cause the flower bud to thoroughly adsorb the fluorescence improver. The flower can be selected from orchids or other flowers as the material in the form of living flowers or separated flowers in a flowering stage with optimal light-colored flower color effects. The original general floral characteristics of the noctilucent flower manufactured by the present invention can be kept, and the noctilucent flower can emit fluorescence in the dark in the night after being irradiated by light to show a characteristic floral appearance and has the advantages of long fluorescence holding time, repeated emersion and greatly improved floral useful value and ornamental value. The manufacturing method of the present invention has the advantages of simplicity, convenience, validity, convenient operation and popularization, no damage to people, livestock and plants and no environmental pollution.
Description
Technical field
The present invention relates to a kind ofly be used for making luminous flowers and plants, especially make the method for noctilucence orchid and make employed fluorescence improver, belong to the technical field of creating special flowers.
Background technology
Novelty is special to be the main trend of current flowers seed selection and sale.Special flowers because of its in different particularity that show in aspect such as form, color and fragrance with common flowers, show very high ornamental value and economic worth.In special flowers, what people dreamed of is QISEHUA and noctilucence orchid, and promptly one presents seven kinds of colors in spending and in noctilucent orchid.These two kinds of special flowers still do not exist at natural world.The characteristics of noctilucence orchid are that flower can be at noctilucence.Consider from the biology angle, can fluorescent bacterias, firefly, jellyfiss body be arranged more noctilucent, they or utilize ambient substance to produce can fluorescigenic organic substance, or utilize fluorescin in the body under the effect of fluorogenic protease and ATP hydrolase and send fluorescence.Also exist the material that can produce fluorescence in plant corpus, as chlorophyll and some quinones substances that widely distribute, they can send buff under the inducing of certain frequency spectrum light source (as ultraviolet ray) or ruddy fluorescence comes.Change the fluorescence protein gene of firefly or jellyfish in the plant mode by technique for gene engineering like this, become the main research approach of creating luminous flowers and plants.
Yet, it is a very tight bioprocess that firefly, jellyfish fluoresce, and except that decomposing fluorescigenic fluorescin (fluorescein), needing provides energy by ATP hydrolase hydrolysising ATP continuously, the fluorescence performance just can be arranged, that is to say that the generation of fluorescence be unable to do without bioenergy.Shift a fluorogene such as GFP and enter in the plant not so difficultly, difficult is to change the ATP hydrolase gene over to and both are had an effect in coordination.In plant inducer fluoresces source such as chlorophyll, quinones substance, mostly need exciting of ultraviolet light in addition, and stronger ultraviolet ray is for the DNA tool sizable harm relevant with vegetable active, so this luminous form is worthless.
Summary of the invention
Technical problem to be solved by this invention is to be the method for utilizing biology, chemistry and physics to combine, and a kind of preparation method and fluorescence improver thereof of luminous flowers and plants is provided.
The technical scheme of preparation method of the present invention is: a, select to have fluorescence protein gene or as the flowers material of autofluorescence carrier, b, preparation fluorescence improver, are coated on fluorescence improver on the flower of flowers at c, allow it fully adsorb.
Press such scheme, described flowers are orchid or other flowers material; The normal anosis worm harm of orchid or other flowers material requirements external form, physiological status is good, and healthy growth is in the flowers plant in flowering stage, and pattern is good with light effect; Orchid comprises Chinese Chunlan, orchid, Moth orchid or kaffir lily, and other flowers comprise rose, chrysanthemum, lily or flameray gerbera etc., to have the plant that changes jellyfish fluorescence protein gene or chimera feature for well; Described flowers are live body flowers or beta pruning is got off from the live body stripped flowers; The flower of orchid or other flowers is spraying or whole the immersion through the method that fluorescence improver handle to apply, and fully is being placed on temperature at 20～25 ℃ behind the absorption fluorescence improver, air humidity illumination 1～2 hour in 75～95% indoor environment.
The technical scheme of fluorescence improver of the present invention is: the biochemical preparation by following component is prepared from:
A, biosurfactant tween (TW) is dissolved in 1: 10～50 ratio in 40～60 ℃ the distilled water, stirs, be cooled to 20～25 ℃,
B, surperficial solubilizer PS is dissolved in 20～25 ℃ of distilled water with 1: 50～200 ratio,
C, preparation phosphate buffer, pH value are 6.8～7.2, and be better with approaching physiological pH value effect of being drawn materials,
D, above-mentioned biosurfactant TW solution and surperficial solubilizer PS solution are fully mixed in about 1: 1: 0.1 ratio with phosphate buffer,
E, fluorescer FR and the above-mentioned mixed liquor that makes mixed in 1: 100～500 ratio get final product.
In the above-mentioned preparation process, add different types of fluorescer and can obtain different types of fluorescence improver, usually can select the hyperfluorescence promoter of two types of HFPR-B or HFPR-G, wherein the fluorescer of HFPR-B adding is FR-B, and the fluorescer that HFPR-G adds is FR-G.
The luminous flowers and plants that the present invention produced has following good effect: the general features that noctilucence orchid of 1, producing and luminous flowers and plants keep former flowers, but, represent a kind of special colored appearance sending fluorescence in the dark at night through after the illumination; 2, above-mentioned fluorescigenic characteristic both can produce on the flowers of live body (complete flowers plant), also can realize in the flowers of exsomatize (cutting off) from plant, and the phosphor persistence time is long, can reproduce repeatedly, thereby improve application and the ornamental value of flowers greatly; 3, preparation method is simple and effective, is convenient to implement and promote, and is harmless to people and animals and plant, environmentally safe.
Embodiment
Be described in further detail embodiments of the invention below.
Embodiment 1:
The blue preparation method of live body noctilucence
A, choose the flowers material
Choose open China blue (as orchid), require the plant strain growth stalwartness, no damage by disease and insect, flowering is normal;
B, preparation fluorescence improver HFPR
Concrete preparation method:
(1) biosurfactant tween (TW) is dissolved in 1: 25 ratio in 40～60 ℃ the distilled water and stirs, be cooled to 20～25 ℃, standby;
(2) surperficial solubilizer PS is dissolved in 20～25 ℃ of distilled water with 1: 80 ratio, standby;
(3) preparation phosphate buffer, pH value be (with better near the physiological PH value effect of being drawn materials) about 6.8～7.2;
(4) above-mentioned biosurfactant TW solution for preparing in advance and surperficial solubilizer PS solution are fully mixed in about 1: 1: 0.1 ratio with phosphate buffer, standby;
(5) an amount of fluorescer FR is mixed in the solution that 1: 350 ratio and step (4) make.
(1) set by step～(5) operation can obtain fluorescence improver HFPR.
The generation of c, live body noctilucence orchid
Place indoorly with growing in China blue in the flowerpot, be sprayed on the flower, maintain the temperature at 20～25 ℃ with fluorescence improver HFPR, humidity is about 85%, placed under the illumination 1～2 hour, in the dark or do not have under the optical condition, China blue becomes the noctilucence orchid with regard to showing fluorescence.They can be used as potted flower.
Embodiment 2:
The preparation method of stripped noctilucence chrysanthemum
A, choose the flowers material
Choose open chrysanthemum (white or yellow all can), require the plant strain growth stalwartness, no damage by disease and insect, flowering is normal;
B, preparation fluorescence improver HFPR
Concrete preparation method:
(1) biosurfactant tween (TW) is dissolved in 1: 30 ratio in 40～60 ℃ the distilled water and stirs, be cooled to 20～25 ℃, standby;
(2) surperficial solubilizer PS is dissolved in 20～25 ℃ of distilled water with 1: 150 ratio, standby;
(3) preparation phosphate buffer, pH value be (with better near the physiological PH value effect of being drawn materials) about 6.8～7.2;
Biosurfactant TW solution that (4) will prepare in advance and surperficial solubilizer PS solution fully mix in about 1: 1: 0.1 ratio with phosphate buffer, and is standby;
(5) an amount of fluorescer FR is mixed in the solution that 1: 500 ratio and step (4) make.
(1) set by step～(5) operation can obtain fluorescence improver HFPR.
The generation of c, the noctilucence chrysanthemum that exsomatizes
(be about 25～30cm), place water, soak flower is whole with fluorescence improver HFPR, it is fully adsorbed, maintain the temperature at 20～25 ℃, humidity placed under the general fluorescent lamp 1～2 hour about 85% to cut the spray of chrysanthemum.Change the chrysanthemum spray over to dark then or do not have under the optical condition, chrysanthemum just can show fluorescence and become the noctilucence chrysanthemum.They can be used as flower arrangement.
Claims (8)
1, a kind of preparation method of luminous flowers and plants is characterized in that: A, select to have fluorescence protein gene or as the flowers material of autofluorescence carrier, B, preparation fluorescence improver, are coated on fluorescence improver on the flower of flowers at C, allow it fully adsorb; Described fluorescence improver is prepared from by the biochemical preparation of following component:
A, the biosurfactant tween is dissolved in 1: 10～50 ratio in 40～60 ℃ the distilled water, stirs, be cooled to 20～25 ℃,
B, surperficial solubilizer is dissolved in 20～25 ℃ of distilled water with 1: 50～200 ratio,
C, preparation phosphate buffer, the pH value is 6.8～7.2,
D, above-mentioned physiology surfactant tween solution and surperficial solubilizer solution are fully mixed in 1: 1: 0.1 ratio with phosphate buffer,
E, fluorescer and the above-mentioned mixed liquor that makes mixed in 1: 100～500 ratio get final product.
2, press the preparation method of the described luminous flowers and plants of claim 1, it is characterized in that described flowers are orchid or other flowers material, the normal anosis worm harm of orchid or other flowers material requirements external form, physiological status is good, healthy growth is in the flowers plant in flowering stage.
3, by the preparation method of the described luminous flowers and plants of claim 2, it is characterized in that described orchid comprises Chinese Chunlan, orchid, Moth orchid or kaffir lily, other flowers comprise rose, chrysanthemum, lily or flameray gerbera.
4,, it is characterized in that described flowers are live body flowers or beta pruning is got off from the live body stripped flowers by the preparation method of claim 1 or 2 described luminous flowers and plants.
5, press the preparation method of the described luminous flowers and plants of claim 2, the flower that it is characterized in that orchid or other flowers is handled the method for coating for spraying or whole the immersion through fluorescence improver, fully be placed on temperature at 20～25 ℃ behind the absorption fluorescence improver, air humidity illumination 1～2 hour in 75～95% indoor environment.
6, a kind of fluorescence improver that is used to make luminous flowers and plants is characterized in that being prepared from by the biochemical preparation of following component:
A, the biosurfactant tween is dissolved in 1: 10～50 ratio in 40～60 ℃ the distilled water, stirs, be cooled to 20～25 ℃,
B, surperficial solubilizer is dissolved in 20～25 ℃ of distilled water with 1: 50～200 ratio,
C, preparation phosphate buffer, the pH value is 6.8～7.2,
D, above-mentioned physiology surfactant tween solution and surperficial solubilizer solution are fully mixed in 1: 1: 0.1 ratio with phosphate buffer,
E, fluorescer and the above-mentioned mixed liquor that makes mixed in 1: 100～500 ratio get final product.
7, by the described fluorescence improver of claim 6, it is characterized in that the biosurfactant tween is dissolved in 1: 25 ratio in 40～60 ℃ the distilled water and stir, be cooled to 20～25 ℃, standby; The ratio of surperficial solubilizer with 1: 80 is dissolved in 20～25 ℃ of distilled water, standby; The preparation phosphate buffer, the pH value is 6.8～7.2; Above-mentioned biosurfactant tween solution for preparing in advance and surperficial solubilizer solution are fully mixed in 1: 1: 0.1 ratio with phosphate buffer, standby; Ratio and the above-mentioned solution that makes of an amount of fluorescer in 1: 350 is mixed.
8, by the described fluorescence improver of claim 6, it is characterized in that the biosurfactant tween is dissolved in 1: 30 ratio in 40～60 ℃ the distilled water and stir, be cooled to 20～25 ℃, standby; The ratio of surperficial solubilizer with 1: 150 is dissolved in 20～25 ℃ of distilled water, standby; The preparation phosphate buffer, the pH value is 6.8～7.2; Above-mentioned biosurfactant tween solution for preparing in advance and surperficial solubilizer solution are fully mixed in 1: 1: 0.1 ratio with phosphate buffer, standby; Ratio and the above-mentioned solution that makes of an amount of fluorescer in 1: 500 is mixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03118971 CN1270606C (en) | 2003-04-18 | 2003-04-18 | Method for making luminous flower and fluorescene improver |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03118971 CN1270606C (en) | 2003-04-18 | 2003-04-18 | Method for making luminous flower and fluorescene improver |
Publications (2)
| Publication Number | Publication Date |
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| CN1449659A CN1449659A (en) | 2003-10-22 |
| CN1270606C true CN1270606C (en) | 2006-08-23 |
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| CN 03118971 Expired - Fee Related CN1270606C (en) | 2003-04-18 | 2003-04-18 | Method for making luminous flower and fluorescene improver |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103299841A (en) * | 2012-03-08 | 2013-09-18 | 台湾糖业股份有限公司 | Method for changing flower color of orchid |
| US20190183060A1 (en) * | 2016-09-06 | 2019-06-20 | Nec Solution Innovators, Ltd. | Glowing plant |
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