CN1269423A - Method of culturing Brazilian bushroom mycelium and extructing proteinpolysaccharide - Google Patents
Method of culturing Brazilian bushroom mycelium and extructing proteinpolysaccharide Download PDFInfo
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- CN1269423A CN1269423A CN 99112812 CN99112812A CN1269423A CN 1269423 A CN1269423 A CN 1269423A CN 99112812 CN99112812 CN 99112812 CN 99112812 A CN99112812 A CN 99112812A CN 1269423 A CN1269423 A CN 1269423A
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- brazilian mushroom
- mushroom mycelium
- polysaccharide protein
- mycelium
- brazilian
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The inoculum of Agaricus blazci Murrill is inoculated on liquid culture medium, sterile air is introduced and cultured for 2-4 weeks to obtain mycelium of Agaricus blazci Murrill and culture liquor, and the mycelium is undergone the processes of heating extraction, filter-concentration, alcohole separation and precipitation and vacuum drying to obtain polysaccharide protein dry powder; and the obtained culture liquor also is undergone the processes of vacuum concentration, alcohol separation and precipitation and centrifugal drying to obtain polysaccharide protein dry powder. Said product is non-toxic, pollution-free, safe and effective, and has no abnormal taste and possesses good anti-cancer effect.
Description
The present invention relates to a kind of extracting method of polysaccharide protein, exactly is to disclose a kind of deep layer to cultivate the method that the Brazilian mushroom mycelium extracts polysaccharide protein.
Brazilian mushroom is the edible mushrooms that originates in Brazil, formal name used at school is Agaricus blazci Murrill, at present, use the cancer resistance polysaccharide protein that the Brazilian mushroom sporophore is extracted, be subjected to the restriction of cultivation condition always, be not easy to technical scale production, product has the peculiar smell of mushroom class, be subject to the pollution of sulfurous gas, make product defective, had a strong impact on the quality of product.
The invention discloses a kind of deep layer and cultivate the method that the Brazilian mushroom mycelium extracts polysaccharide protein, purpose is intended to solve the problem of its technical scale production.
At purpose of the present invention, the technical solution scheme that adopts is: utilize fermentation engineering to use aseptic liquid nutrient medium in bio-reactor, insert Brazilian mushroom Agaricus blazci Murrill bacterial classification, supply with sterile air, keep suitable temperature, carry out deep layer and cultivate, obtain Brazilian mushroom mycelium and Brazilian mushroom mycelium culture liquid, extract polysaccharide protein respectively again from mycelium and Brazilian mushroom mycelium culture liquid, concrete technology is as follows:
1, gets glucose 3～5%, peptone 0.03～0.05% and water and be mixed with liquid nutrient medium, through 120 ℃ of temperatures power 1.5kg/cm
2Sterilized 30 minutes for real jar, be cooled under 30 ℃ of conditions, insert Brazilian mushroom (Agaricus blazci Murrill) bacterial classification, feed sterile air, air flow is 1: 0.5/v/v, cultivated for 2～4 weeks, middle 20% liquid nutrient medium that replenishes, obtain Brazilian mushroom mycelium culture liquid and Brazilian mushroom mycelium, the Brazilian mushroom mycelium is globe.
2, with the mycelium nutrient solution vacuum concentration of above-mentioned cultivation to 1/3 of original volume, add the ethanol of 1～3 times of amount of volume, precipitate 24 hours, the centrifuging and taking throw out, vacuum-drying both must the polysaccharide protein powder.
3, the water that the Brazilian mushroom mycelium of gained is added 6～10 times of amounts of its volume, extracted 3 hours through 100 ℃ of heating, again filtrate is concentrated into 1/3 of original volume after the filtration, the ethanol that adds 1～3 times of amount of its volume again, alcohol was analysed precipitation 24 hours, taking precipitate under vacuum condition dry 70 ℃ promptly gets polysaccharide protein dry powder.
Positively effect of the present invention is: adopt the fermentation engineering deep layer to cultivate Brazilian mushroom, be not subjected to the influence of cultivation condition, nature, geographical environment, climatic season, large-scale utilization fermentation equipment suitability for industrialized production polysaccharide protein, product of the present invention is water-soluble taupe dry powder, contain dextran and protein, infrared absorption spectrum with polysaccharide feature, product free from extraneous odour, nontoxic, pollution-free, oral safe and effective, has fabulous anticancer effect, through the antitumor test of mouse S-180, tumour inhibiting rate is 66～86%, and immunity studies show that this product can activate, swash
Scavenger cell alive strengthens macrophage function, can be developed into cancer therapy drug and anti-cancer health-care food.
Further specify the present invention below in conjunction with embodiment:
Embodiment 1
A, get glucose 5%, peptone 0.05% and water and be mixed with liquid nutrient medium 500 liters, through 120 ℃ of pressure 1.5kg/cm
2Sterilized 30 minutes for real jar, be cooled to 28 ℃, Brazilian mushroom bacterial classification 50 liters are inoculated on this liquid nutrient medium, feed sterile air, temperature keeps 28 ℃, cultivates 18 days, middle additional liquid nutrient medium 100 liters obtain Brazilian mushroom mycelium 30kg and Brazilian mushroom nutrient solution 300 liters;
B, with the Brazilian mushroom mycelium 30kg that obtains, add the water of 180kg, extracted 3 hours through 100 ℃ of heating, filtrate is concentrated into 1/3 of original volume after the filtration again, adds the long-pending ethanol of triploid again, alcohol was analysed precipitation 24 hours, taking precipitate is 70 ℃ of dryings under vacuum condition, get polysaccharide protein dry powder 60 grams;
C, with liquid nutrient medium 300 liter vacuum concentration to 100 liters that obtain, add 150 liter ethanol, alcohol was analysed 24 hours, through 2000rmp/min centrifugal 15 minutes, taking precipitate vacuum-drying, polysaccharide protein dry powder 265 grams, this product is water-soluble taupe dry powder, free from extraneous odour.
Embodiment 2
A, get liquid nutrient medium 300 liters of glucose 5%, yeast extract paste 0.05% and water preparation, inoculation Brazilian mushroom bacterial classification 30 liters feed sterile air, keep 25～30 ℃ of temperature, cultivate 21 days, obtain Brazilian mushroom mycelium 25kg and nutrient solution 150 liters;
B, get above-mentioned mycelium 25kg and add water 250kg, heat 100 ℃ and extracted 3 hours, again filtrate is concentrated into 100kg after the filtration, add the ethanol of 300kg, alcohol was analysed precipitation 24 hours, and taking precipitate is 70 ℃ of dryings under vacuum condition, polysaccharide protein dry powder 40 grams;
C, during with nutrient solution 150 liter vacuum concentration to 50 liters that obtain, add the ethanol of 150 liters, centrifugal 20 minutes of last whizzer 2500rmp, taking precipitate are dry under vacuum condition, polysaccharide protein dry powder 225 grams.
Claims (1)
1, a kind of deep layer is cultivated the method that the Brazilian mushroom mycelium extracts polysaccharide protein, at first with on Brazilian mushroom (Agaricus blazci Murrill) the bacterial classification inoculation liquid medium within, feed sterile air, carrying out deep layer cultivates, obtain Brazilian mushroom mycelium culture liquid and Brazilian mushroom mycelium, can extract polysaccharide protein from Brazilian mushroom mycelium nutrient solution or Brazilian mushroom mycelium, concrete technology is as follows;
A, with above-mentioned Brazilian mushroom mycelium nutrient solution vacuum concentration to 1/3 of original volume, add the ethanol of 1～3 times of amount of volume, precipitate 24 hours, the centrifuging and taking throw out, vacuum-drying both must the polysaccharide protein powder;
B, above-mentioned Brazilian mushroom mycelium added the water of 6～10 times of amounts of its volume, extracted 3 hours through 100 ℃ of heating, again filtrate is concentrated into 1/3 of original volume after the filtration, at the ethanol that adds 1～3 times of amount of its volume, alcohol was analysed precipitation 24 hours, drying is 70 ℃ under vacuum condition, gets polysaccharide protein dry powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99112812 CN1269423A (en) | 1999-04-02 | 1999-04-02 | Method of culturing Brazilian bushroom mycelium and extructing proteinpolysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99112812 CN1269423A (en) | 1999-04-02 | 1999-04-02 | Method of culturing Brazilian bushroom mycelium and extructing proteinpolysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1269423A true CN1269423A (en) | 2000-10-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99112812 Pending CN1269423A (en) | 1999-04-02 | 1999-04-02 | Method of culturing Brazilian bushroom mycelium and extructing proteinpolysaccharide |
Country Status (1)
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| CN (1) | CN1269423A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100340186C (en) * | 2004-12-14 | 2007-10-03 | 复旦大学 | Himematsutake water soluble polysaccharide and its preparing process and use |
| US7514085B2 (en) | 2004-07-16 | 2009-04-07 | Medimush A/S | Immune modulating compounds from fungi |
| CN102823937A (en) * | 2012-08-16 | 2012-12-19 | 湖北中烟工业有限责任公司 | Extracting method of submerged fermentation mycelium extractive of lepista nuda and application thereof in cigarettes |
| CN104187624A (en) * | 2014-08-07 | 2014-12-10 | 逯明福 | Comprehensive nutrition powder and preparation method thereof |
| US9072776B2 (en) | 2005-06-15 | 2015-07-07 | Glycanova As | Anti-cancer combination treatment and kit-of-parts |
| CN104922161A (en) * | 2015-06-01 | 2015-09-23 | 吉林农业大学 | Lentinus lepideus polysaccharide protein compound, and preparation method and application thereof |
| CN104922162A (en) * | 2015-06-01 | 2015-09-23 | 吉林农业大学 | Willow needle mushroom glycocalyx, as well as preparation method and application thereof |
-
1999
- 1999-04-02 CN CN 99112812 patent/CN1269423A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7514085B2 (en) | 2004-07-16 | 2009-04-07 | Medimush A/S | Immune modulating compounds from fungi |
| US7682615B2 (en) | 2004-07-16 | 2010-03-23 | Beka Holding As | Immune modulating compounds from fungi |
| CN100340186C (en) * | 2004-12-14 | 2007-10-03 | 复旦大学 | Himematsutake water soluble polysaccharide and its preparing process and use |
| US9072776B2 (en) | 2005-06-15 | 2015-07-07 | Glycanova As | Anti-cancer combination treatment and kit-of-parts |
| CN102823937A (en) * | 2012-08-16 | 2012-12-19 | 湖北中烟工业有限责任公司 | Extracting method of submerged fermentation mycelium extractive of lepista nuda and application thereof in cigarettes |
| CN102823937B (en) * | 2012-08-16 | 2014-12-10 | 湖北中烟工业有限责任公司 | Extracting method of submerged fermentation mycelium extractive of lepista nuda and application thereof in cigarettes |
| CN104187624A (en) * | 2014-08-07 | 2014-12-10 | 逯明福 | Comprehensive nutrition powder and preparation method thereof |
| CN104922161A (en) * | 2015-06-01 | 2015-09-23 | 吉林农业大学 | Lentinus lepideus polysaccharide protein compound, and preparation method and application thereof |
| CN104922162A (en) * | 2015-06-01 | 2015-09-23 | 吉林农业大学 | Willow needle mushroom glycocalyx, as well as preparation method and application thereof |
| CN104922162B (en) * | 2015-06-01 | 2019-02-26 | 吉林农业大学 | A kind of Liu Sheng flammulina velutipes albumen composition and preparation method and application |
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