CN1268172A - Novel lactic acid bacteria - Google Patents

Novel lactic acid bacteria Download PDF

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CN1268172A
CN1268172A CN98807912A CN98807912A CN1268172A CN 1268172 A CN1268172 A CN 1268172A CN 98807912 A CN98807912 A CN 98807912A CN 98807912 A CN98807912 A CN 98807912A CN 1268172 A CN1268172 A CN 1268172A
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milk
acid bacteria
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dental plaque
food
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钟淑五
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Abstract

Enterococcus spp. 1357, Lactobacillus spp. V20 and Lactococcus spp. 1370, and H2O2-producing Streptococci have a potent and lasting inhibitory activity on the production of water-insoluble glucan (mutan) and dental plaque in human mouth, or the growth of anaerobic bacteria causing gingivitis, periodontitis, and accompanied halitosis.

Description

New milk-acid bacteria
Background of invention
1. invention field
The present invention relates to produce in some mouths that are suppressed at the people the new milk-acid bacteria of dental plaque.More particularly, can suppress the generation of the main component of this dental plaque of water-fast dextran (mutan) by described new bacterium, described dextran (mutan) is bacteriogenic by what suppress in people's mouth usually.The oral cavity anaerobium that also can suppress to cause gingivitis, periodontitis and the property followed halitosis (stench) by described new bacterium.These genus lactubacillus are in Enterococcus spp., Lactobacillus spp., Lactococcusspp. and Streptococcus spp., and they suppress the generation of water-fast dextran or resist the bacterium that plays a role or suppress to cause the growth of the anerobe of gingivitis and periodontitis in the generative process of water-fast dextran.
2. the description of prior art
Milk-acid bacteria generally makes carbohydrate fermentation become lactic acid.Milk-acid bacteria survives in the oral cavity and digestive tube of humans and animals, and is used to produce leavened food, such as sour milk, cheese etc.Use them to produce biologically active substance such as medicine in addition.The bacterium that representative these produce lactic acid is thermophilus streptococcus, enterococcus faecalis, Enterococcus durans, Lactococcus lactis, Lactobacterium acidophilum, lactobacillus bulgaricus, lactobacterium casei and plant lactobacillus.As the sojourner in the humans and animals internal organ, known these Gram-positive milk-acid bacterias suppress lactic acid and the antimicrobial substance that pathogenic bacteria grows by producing, thereby play an important role in the process that keeps internal organ health.
The most important composition of dental plaque is a dextran.Dextran or for water-soluble dextran, promptly have 1,6-α key be as the dextran of main chemical bond, or for having 1, and 3-α key is as the main water-fast dextran (mutan) of chemical bond.Solubleness in the water and 1, the quantity of 3-α key is inversely proportional to.Therefore, water-fast dextran (mutan) is as the main matrix of dental plaque.With regard to the ability of prevention dental plaque, tested the dextranase (α-1,6 glucan hydrolase) of digestion dextran.But dextranase is problematic (" basic tooth microbiology ": Appleton ﹠amp to the Evaluation on effect of prevention dental plaque; Lange, Norwalk, SanMateo, p.337, and 1991), this is because dextranase can not digest the main matrix of this dental plaque of mutan.It is found that the MUTANASE (inscribe-α-1,3-dextranase) of decomposing mutan has some effects to the digestion of dental plaque.But MUTANASE is unimportant to the Decomposition of dental plaque, and the time that need cost a lot of money is expressed its effectiveness.Therefore find that these enzymes have slight effect for the formation of dental plaque in people's the oral cavity.
With regard to prevent dental plaque by milk-acid bacteria with regard to, publication number is that the european patent application of 0-524-732-A2 discloses the purposes that can produce the streptococcus-salivarius of dextranase outside born of the same parents.But because dextranase can not digest the main matrix of this dental plaque of mutan, so the effect of its prevention dental plaque is problematic.Streptococcus-salivarius is not used as the initial bacterium of fermented milk.
Summary of the invention
Owing to deeply and completely studying that the inhibition activity of generation that milk-acid bacteria is stoped water-fast dextran or dental plaque and anerobe growth is carried out, this research is based on a kind of like this supposition, more promptly is lodged in generation or the inhibition that milk-acid bacteria in people's the mouth may be able to suppress water-fast dextran or dental plaque and causes the growth of the anerobe of gingivitis and periodontitis.By many clinical trials, have been found that and confirmed that these new bacterial strains have the ability of the anerobe growth that the generation that suppresses water-fast dextran or dental plaque significantly and inhibition cause gingivitis and periodontitis.They are called Enterococcus spp.1357, Lactobacillus spp.V20 and Lactococcus spp.1370 respectively.At present on July 30th, 1997 and December 11 they are deposited in Korea S's bio-science and Bioteknologisk Institut Korea S's type culture preservation in (preserving number of Enterococcus spp.1357 is KCTC 0360BP, the preserving number of Lactobacillus spp.V20 is KCTC 0361BP, and the preserving number of Lactococcus spp.1370 is KCTC 0415BP).
Dextran or be water-soluble or be water-fast (mutan), but every kind all be by by Streptococcus mutans this in forming the bacterium of dental plaque most important bacterium excretory Transglucosylase, from the sucrose synthetic.But, water-fast dextran is only arranged, is that mutan is the main matrix of dental plaque.
Enterococcus spp.1357, Lactobacillus spp.V20 and such as the such generation H of Streptococcus oralis, gentle suis, Streptococcus mitis and Streptococcus sanguis 2O 2Bacterium (" uncle's Jie Shi classification bacteriology handbook the 2nd volume: Williams of streptococcus; Wilkins, Baltimore, London, Los Angeles, Sydney, 1986) Streptococcus mutans is had growth inhibitory activity.When in meat soup, producing H with Enterococcus spp.1357, Lactobacillus spp.V20 or conduct 2O 2The Streptococcus oralis (ATCC 35037) of representative of streptococcus bacterium when cultivating Streptococcus mutans together, the quantity that Streptococcus mutans forms bacterium colony is reduced to one of about percentage of the colony counts of single culture Streptococcus mutans.Because to the restraining effect of Streptococcus mutans, the generation of water-fast dextran or dental plaque also obviously has been subjected to inhibition.
When in the culture broth that the high-molecular weight dextran is joined Streptococcus mutans, disturbed and water-fast dextran bonded Transglucosylase, synthetic (the people such as Shigeyuki H. who suppresses ensuing water-fast dextran then, " general microbiology magazine " 116:51,1980).Lactococcus spp.1370 has produced a large amount of water miscible dextran; When cultivating Streptococcus mutans with this Lactococcus spp.1370, water-fast dextran synthetic has been subjected to inhibition.
In general, the dental plaque that adheres on the dental surface provides a suitable place, breed and cause forming carious tooth on this place's Streptococcus mutans and other bacterium, so a purpose of this research is to find can suppress to be generated by the Streptococcus mutans in the mouth the new bacterium of water-fast dextran or dental plaque.
Anerobe appears in oral cavity periodontitis and the gingivitis with very high ratio.Along with the increase of disease severity, the ratio of anerobe significantly increases (90% micro-flora is arranged approximately) in the periodontitis illness.The main anerobe that causes gingivitis is fertile Salmonella of middle Prey and Fusobacterium nucleatum.Cause the main anerobe of periodontitis to comprise porphyromonas gingivalis, actinobacillus actinomycetem comitans, the fertile Salmonella of middle Prey and tool nuclear shuttle bar mattress (" contemporary oral microorganism is learned and immunology " Mosby-Year Book, St. Louis, 1992).These anerobes produce the malodorous composition such as volatile sulfur compounds in mouth.Main volatile sulfur compounds is from the hydrogen sulfide of L-halfcystine and from the thiomethyl alcohol of L-methionine(Met) (Persson, S., " oral microorganism is learned and immunology " 7:278,1992).Lactobacillus spp.V20 and such as the such generation H of Streptococcus oralis, gentle suis, Streptococcus mitis and Streptococcus sanguis 2O 2The bacterium of streptococcus generated the hydrogen peroxide of the anerobe growth that suppresses to cause gingivitis and periodontitis, thereby improve and prevented illness and reduced the halitosis of being followed.Hydrogen peroxide is the oxygenant that a kind of S-S form that changes into oxidation by general-SH functional group makes enzyme deactivation, and as the sterilizing agent of antibacterium, especially anaerobe resistant.
Another purpose of this research provides the Foods or drinks that has used described milk-acid bacteria.When having eaten the food that contains described milk-acid bacteria, milk-acid bacteria wherein can directly suppress the generation of water-fast dextran and have the activity that suppresses its growth to forming the microorganism that dental plaque makes contributions, described milk-acid bacteria can suppress the formation of dental plaque naturally, and has finally prevented the generation of carious tooth.And when food contains the milk-acid bacteria of the anerobe growth that can suppress to cause gingivitis and periodontitis, the halitosis that described milk-acid bacteria can improve and prevent gingivitis, periodontitis and follow naturally.
Accompanying drawing is briefly described
By with reference to appended accompanying drawing, will be obviously from the description of following specific embodiments more than the present invention and other purpose and aspect, appended accompanying drawing shows the restraining effect (Fig. 1) of described new bacterial strain to artificial dental plaque generation on positive tooth silk.
Detailed Description Of The Invention
From human body, take out milk-acid bacteria,, and under 37 ℃, cultivate its streak inoculation on brain heart infusion agar.Afterwards, test the generation whether isolated bacterial colony can suppress the water-fast dextran of Streptococcus mutans (Ingbritt bacterial strain) production.In a sample pool, the culture broth of 0.1mL Streptococcus mutans and the isolated bacterium of 0.1mL is inoculated in the brain heart infusion agar of the 3mL that is supplemented with 0.5% yeast extract and 5% sucrose.In contrast, Streptococcus mutans is inoculated into separately in the brain heart infusion agar that contains yeast extract and sucrose.For making Streptococcus mutans produce water-fast dextran, be 30 ° angle placement sample pool and descend cultivation 1 day at 37 ℃ with horizontal plane with incubator.After removing culture broth, with 4mL distilled water wash sample pool, the 3mL distilled water of packing into then.By its light absorption value (OD) under 550nm of spectrophotometer measurement.Because OD value is directly proportional with the water-fast dextran that produces, thus isolate produced obvious lower OD value compared with the control bacterium as dental plaque inhibition bacterial strain.
In order to estimate the ability that produces hydrogen peroxide, with isolated bacterial strain streak inoculation to containing 0.25mg/mL3,3 ', 5, on the brain heart infusion agar of the dihydrochloride of 5 '-tetramethyl-benzidine and 0.01mg/mL horseradish peroxidase, and in the anaerobism incubator, cultivated 48 hours.Lactobacillus spp.V20 has formed blue bacterium colony, and this shows that Lactobacillus spp.V20 has the ability of producing hydrogen peroxide.
Studied the Microbiological Characteristics of isolated bacterial strain, such as morphology and physiology characteristic (table 1) and the metabolic ability of sugar decomposition (table 2).
Table 1
Morphology and physiology characteristic
Characteristic Isolated bacterial isolates
Enterococccus?spp. ???????1357 Lactobacillus?spp. ??????V20 ?Lactobacillus?spp. ?????????1370
Morphology Coccus, chain Bacillus, chain Coccus, chain
Gramstaining Positive Positive Positive
The formation of spore ????- ????- ????-
Catalatic activity ????- ????- ????-
Cultivation under 10 ℃ of temperature ????+ ????- ????+
Cultivation under 45 ℃ of temperature ????+ ????+ ????-
pH9.6 ????+ ????- ????-
40% bile acide ????+ ????- ????+
6.5％NaCl ????+ ????- ????-
Growth on the MRS substratum ????- ????+ ????-
The production of 3-oxobutanol ????+ ????+
The hydrolysis of urobenzoic acid ????+ ????-
Pyrrolidone-base (pyrrolidonylary) Ntn hydrolase ????+ ????-
Alpha-galactosidase ????- ????-
Beta-Glucuronidase ????- ????-
Beta-galactosidase enzymes ????+ ????-
Alkaline phosphatase ????- ????-
The leucine Arylamidase ????+ ????+
Arginine dihydrolase ????+ ????-
Table 2
Catalytic activity to sugar
Carbohydrate Isolated bacterial isolates
Enterococcus?spp. ??????1357 ?Lactobacillus?spp. ????????V20 ??Lactococcus?spp. ????????1370
Pectinose ????- ????- ????-
Amygdalin ????+ ????- ????-
Cellobiose ????+ ????+ ????+
Vitamin C2 ????+ ????+ ????-
Fructose ????+ ????+ ????+
Semi-lactosi ????+ ????+ ????+
Glucose ????+ ????+ ????+
Lactose ????+ ????+ ????+
Maltose ????+ ????+ ????+
Mannitol ????- ????- ????+
Seminose ????+ ????+ ????+
Melizitose ????- ????- ????-
Raffinose ????- ????- ????-
Rhamnosyl ????- ????- ????-
Saligenin ????+ ????- ????-
Sorbitol Powder ????- ????- ????-
Trehalose ????+ ????+ ????+
Inulin ????- ????-
Starch ????+ ????+
Glycogen ????- ????-
As mentioned above, compared with the control in in-vitro separation and measured and to have suppressed the new milk-acid bacteria that dental plaque produces.And further tested new characteristic in vivo, be about to isolated new bacterium and apply in people's the oral cavity.
Example I
In disposable sample pool, suppress the generation of water-fast dextran
With the M17 substratum of equivalent and the MRS substratum mixes and replenish 0.5% yeast extract, 5% sucrose and 0.1M TES (pH 8.0).In 3 milliliters of media transfer to that are mixed with disposable sample pool, in this sample pool, inoculate the overnight culture of 75 μ l Streptococcus mutans then.In incubator, place sample pool, and cultivated 1 day down at 30 ℃ with angle horizontal by 30 °.Remove inclusion, use 3mL distilled water wash sample pool then.Afterwards, fill sample pool with the distilled water of 4mL, and by the light absorption value under the spectrophotometer measurement 550nm.Repeat this measurement three times and obtained mean value (table 3).
Table 3
The restraining effect that milk-acid bacteria produces water-fast dextran
Sample The bacterial isolates of test ??OD(550nm)
Contrast I Streptococcus mutans ????2.122
Contrast II Thermophilus streptococcus+Streptococcus mutans ????2.325
The I group Enterococcus?spp.1357 ????0.434
The II group Enterococcus spp.1357+ Streptococcus mutans ????0.713
The III group Lactobacillus?spp.V20 ????0.506
The IV group Lactobacillus spp.V20+ Streptococcus mutans ????1.154
The V group Lactococcus?spp.1370 ????0.576
The VI group Lactococcus spp.1370+ Streptococcus mutans ????1.020
The VII group Streptococcus oralis ????0.511
The VIII group Streptococcus oralis+Streptococcus mutans ????0.980
Just as shown in table 3, the light absorption ratio of the optical density(OD) of I control group and II control group at the 550nm place is 2.122 and 2.325, and the optical density(OD) of II test group, IV test group, VI test group and VIII test group is respectively 0.713,1.154,1.020 and 0.980.The reduction of this light absorption ratio shows that these bacteriostatic Streptococcus mutans produce water-fast dextran.
Example II
On positive tooth silk, form artificial dental plaque
With the M17 substratum of equivalent and the MRS substratum mixes and replenish 0.5% yeast extract, 5% sucrose and 0.1MTES (pH8.0).150 milliliters of substratum that are mixed with are poured in the beaker.The positive tooth silk of 0.016 inch stainless steel (45mg) is immersed in the substratum.With every mL substratum is 2.5 * 10 6Concentration inoculation Streptococcus mutans.Afterwards, exceed with concentration than Streptococcus mutans 5 times concentration with the inoculation of milk-acid bacteria in substratum, and under 37 ℃, in vibration, in CO 2Cultivated 6.5 hours in the incubator.Water-fast dextran or dental plaque are only arranged attached to (McCabe, people such as R.M., " Archs oralBoil. " 12:1653,1967) on the silk.Transfer in the fresh beaker described silk and take pictures (Fig. 1).Fig. 1 (A) is the photo that the culture of Streptococcus mutans is only arranged, and Fig. 1 (B) is the photo of the coculture of Streptococcus mutans and Lactococcus spp.1370.
The weight of the artificial spot that measurement forms on silk, and the results are shown in the table 4.
Table 4
Milk-acid bacteria is to forming the inhibition activity of artificial spot
Sample The bacterial isolates of test The weight of the spot that produces
Contrast I Streptococcus mutans ????75.4mg
Contrast II Thermophilus streptococcus+Streptococcus mutans ????92.3mg
The I group Enterococcus spp.1357+ Streptococcus mutans ????0.0mg
The II group Lactobacillus spp.V20+ Streptococcus mutans ????30.9mg
The III group Lactococcus spp.1370+ Streptococcus mutans ????0.0mg
The IV group Streptococcus oralis+Streptococcus mutans ????0.0mg
In I control group and II control group, form the artificial spot of 75.4mg and 92.3mg, and in I test group, III test group and IV test group, do not formed artificial spot.In the II test group, the weight of spot is reduced to 30.9mg.Therefore, this just clearly illustrates that these bacteriums have potential for the dental plaque that is produced by Streptococcus mutans and suppress active.
EXAMPLE III
The dental plaque exponential descends in people's the mouth
In order to estimate the effect that new lactic bacterium strains reduces the dental plaque index in the population, in the middle of 38 people, test, so that by Quigley and Hein dental plaque index (Harper, people such as D.S., " periodontology magazine " 61:352,1990) obtain the score of dental plaque.
38 ages are in this research of grownup's voluntary participation of 22 to 26 years old youth.All volunteers have accepted comprehensive oral prophylaxis, and have suspended all oral hygienes.The volunteer but stops to brush teeth with toothbrush as food and drink at ordinary times.Evaluation primary dental plaque score in the 24th hour after accepting oral prophylaxis.After all dental plaques of opening except that third molar, calculate the score of dental plaque by Quigley and Hein dental plaque index.The volunteer is distributed into two groups (every group is 19 people) randomly, and the I group is gargled with Lactococcus spp.1370, and the II group is gargled with Lactobacillus spp.V20.The suspension of the bacterium of test is by cultivating Lactococcus spp.1370 or preparing in Lactobacillus spp.V2024 hour in milk.The volunteer gargles once mouth immediately after oral prophylaxis, and uses 20mLLactococcus spp.1370 or the Lactobacillus spp.V20 culture (10 in milk after the meal immediately 9CFU/mL) gargle twice mouthful, gargled 2 minutes altogether.After accepting oral prophylaxis, evaluate the score of dental plaque in the 24th hour once more.In each group, the dental plaque score of all teeth except that third molar is averaged and carried out statistical analysis.The result shows after accepting oral prophylaxis in the 24th hour, in the I group dental plaque index decreased 0.97,0.55 (table 5) descended in the II group.It is significant (p＜0.05) statistically that the dental plaque exponential reduces, and promptly Lactococcus spp.1370 and Lactobacillus spp.V20 have reduced the formation of dental plaque in the oral cavity significantly.
Table 5
Cause the dental plaque exponential to descend by bacterium mouth-washes
Used bacterium The mean value of primary dental plaque score At the mean value of back dental plaque score of gargling with bacterium Deviation
??Lactococcus?spp.1370 ????2.17 ????1.20 ??-0.97 *
??Lactobacillus?spp.V20 ????2.15 ????1.60 ??-0.55 *
*P＜0.01 is by joining paired t check checking
EXAMPLE IV
In the blended culture, suppress the growth of anerobe
In the MRS substratum, cultivated Lactobacillus spp.V2024 hour.In the anaerobism incubator, in anaerobic bacteria culture meat soup, cultivated fertile Salmonella of porphyromonas gingivalis, middle Prey and Fusobacterium nucleatum 36 hours, described culture broth contains brain heart infusion agar 18.5g, yeast extract 5.0g, teichmann's crystals solution 10mL (teichmann's crystals of 50mg is dissolved in the sodium hydroxide solution of 1mL 1N, and adds the distilled water of 100mL) and vitamin K solution 0.2mL (the vitamin K solution of 0.15mL is mixed with the ethanol of 30mL 95%) for every liter.Cultivated actinobacillus actinomycetem comitans 36 hours in the anaerobism incubator in the TSBV substratum, described substratum contains trypticase soy broth 30g, yeast extract 1.0g, horse serum 100mL, bacitracin 75mg and vancomycin 5mg for every liter.With the culture suspension of the 0.1mL of Lactobacillus spp.V20 and various anerobes with every mL 1.4 * 10 8Concentration separately or combined inoculation in substratum, this substratum contains anaerobic bacteria culture meat soup or the TSBV substratum with the MRS meat soup blended 3.7mL of 0.3mL, and cultivates 36 hours in the anaerobism incubator.Culture suspension is diluted and be inoculated into MRS agar, contain on the anaerobic bacteria culture agar or TSBV agar of 3% sheep blood.In the 72nd hour, number goes out the quantity of bacterium colony after cultivation.After carrying out single culture, the colony-forming unit of Lactobacillus spp.V20 and various anerobes all increases to some extent, and after Lactobacillus spp.V20 cultivates, but can not find the bacterium colony of various anerobes.When cultivating anerobe with the lactobacterium casei that does not produce hydrogen peroxide, colony-forming unit does not significantly reduce (table 5).When cultivating as producing H with various anerobes by aforesaid method 2O 2The Streptococcus oralis (ATCC 35037) of streptococcic representative the time, anerobe does not form bacterium colony on substratum.
Table 6
Colony-forming unit after the cultivation
The bacterium of inoculation The colony-forming unit of Bacterium lacticum after cultivating (/mL) The colony-forming unit of anerobe after cultivating (/mL)
Lactobacillus?spp.V20 ????8.2×10 8
Lactobacterium casei ????9.0×10 8
Porphyromonas gingivalis ????1.9×10 8
Actinobacillus actinomycetem comitans ????2.0×10 8
The fertile Salmonella of middle Prey ????1.8×10 8
Fusobacterium nucleatum ????1.8×10 8
Lactobacillus spp.V20+porphyromonas gingivalis ????8.0×10 8 ????0
Lactobacillus spp.V20+actinobacillus actinomycetem comitans ????8.8×10 8 ????0
Lactobacillus spp.V20+middle Prey is irrigated Salmonella ????8.9×10 8 ????0
Lactobacillus spp.V20+Fusobacterium nucleatum ????7.8×10 8 ????0
Lactobacterium casei+porphyromonas gingivalis ????9.1×10 8 ????1.5×10 8
Lactobacterium casei+actinobacillus actinomycetem comitans ????9.3×10 8 ????1.8×10 8
Lactobacterium casei+middle Prey is irrigated Salmonella ????8.9×10 8 ????1.6×10 8
Lactobacterium casei+Fusobacterium nucleatum ????9.8×10 8 ????1.6×10 8
The back has provided some examples, has in fact used described new milk-acid bacteria in these examples.Purposes example I: sour milk
The broth culture that just in time will contain described new lactic bacterium strains before fermentation adds in the food with the amount of 0.1% (volume), and ferments with existing bacterium and to produce sour milk food.The sour milk food that obtains comments flavor person to taste by 10.They point out not have the difference of local flavor between specimen and the food (contrast) that is obtained commercially.
Before the sealing step of production process, add described lactic bacterium strains with the amount of 0.2% (volume).From participated in 10 of tasting test comment summed up the flavor person these specimen that so obtain on taste with control food and indiscriminate reflection.Purposes example II: cream
Before packaging step, the freeze dried lactic bacterium strains of 0.2% (weight) is joined in the cream of producing by ordinary process.Provide these creams that so obtain as the sample of tasting.Purposes EXAMPLE III: cheese
Before packaging step, the freeze dried lactic bacterium strains of 0.2% (weight) is joined in the cheese food of producing by ordinary process.Provide these cheese foods that so obtain as the sample of tasting.Purposes EXAMPLE IV: freeze dried milk-acid bacteria
Cultivate described new milk-acid bacteria and freeze-drying (lyophilize).Can take in these freeze dried bacteriums that are capsule, sheet and small packages form separately or with other bacterium or material.Provide these freeze dried products that so obtain as the sample of tasting.
Therefore just described lactic bacterium strains is applied in the various food, comprising lozenges, shortening, ice-creams, oleomargarine, bubble sauerkraut or the like.
From top embodiment, apparent described new lactobacillus strain has potential and persistent inhibition activity for the generation of water-fast dextran or dental plaque in people's mouth or to the growth of the anerobe that causes gingivitis, periodontitis and the property followed halitosis.
In addition, find that described new bacterial strain can be applied in the various food and can be applied directly on the tooth.
According to above-mentioned technology, many improvement of the present invention and variation all are possible.

Claims (9)

1, a kind of milk-acid bacteria, described milk-acid bacteria perhaps have forming the growth-inhibiting effect of the bacterium of dental plaque in the oral cavity by influencing the generation that Transglucosylase suppresses water-fast dextran (mutan) or dental plaque.
2, according to the milk-acid bacteria of claim 1, described milk-acid bacteria is Enterococcus spp.1357 (KCTC0360BP), Lactobacillus spp.V20 (KCTC 0361BP) and Lactococcus spp.1370 (KCTC0415BP).
3, a kind of milk-acid bacteria, described milk-acid bacteria inhibition causes the growth of the anerobe of gingivitis, periodontitis and the property followed halitosis (stench).
4, a kind of milk-acid bacteria, described milk-acid bacteria suppresses hydrogen peroxide (H 2O 2), thereby the growth of the anerobe of inhibition claim 3.
5, according to the milk-acid bacteria of claim 4, described milk-acid bacteria is Lactobacillus spp.V20 (KCTC0361BP) and Streptococcus oralis (ATCC 35037).
6, a kind of food, described food can be by influencing the generation that Transglucosylase suppresses water-fast dextran or dental plaque, perhaps can suppress to form the growth of the bacterium of dental plaque, described food contains the described lactic bacterium strains of claim 1 or its mixture.
7, a kind of food, described food can suppress to cause the growth of the anerobe of gingivitis, periodontitis and the property followed halitosis, and described food contains the described lactic bacterium strains of claim 4 or its mixture.
8, according to the food of claim 6 or 7, described food can be sour milk, cream, cheese, ice-creams, lozenges, shortening, oleomargarine or bubble sauerkraut.
9, freeze dried claim 1 or 4 described milk-acid bacterias, it is capsule, sheet or small packages form.
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