CN1263720A - 黄曲霉毒素b1解毒酶去除黄曲霉毒素b1的方法 - Google Patents

黄曲霉毒素b1解毒酶去除黄曲霉毒素b1的方法 Download PDF

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CN1263720A
CN1263720A CN99117161A CN99117161A CN1263720A CN 1263720 A CN1263720 A CN 1263720A CN 99117161 A CN99117161 A CN 99117161A CN 99117161 A CN99117161 A CN 99117161A CN 1263720 A CN1263720 A CN 1263720A
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afb1
toxenzyme
aflatoxin
separated
toxic
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CN1085057C (zh
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刘大岭
姚冬生
黄炳贺
古练权
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Guangdong-Prov. Technology Development Center
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Abstract

本发明提供一种用黄曲霉毒素B1解毒酶去除黄曲霉毒素B1的方法,其特征在于将该解毒酶直接或间接作用于含毒的样品,缓冲液呈pH7.0—8.5,在20—70℃作用5—120分钟。该解毒酶能够破坏黄曲霉毒素B1的致毒、致癌活性。本发明方法专一,高效,温和和安全,适用于食品加工业去除黄曲霉毒素。

Description

黄曲霉毒素B1解毒酶去除黄曲霉毒素B1的方法
本发明涉及一种黄曲霉毒素B1解毒酶的使用方法，将黄曲霉毒素B1解毒酶用于对黄曲霉毒素B1的解毒。黄曲霉毒素B1是所有真菌毒素中毒性最强一种，常见于谷物、豆类、植物种子如花生瓜子等农作物及其制品如酱油、醋、啤酒、花生油等等，还见于牛奶、羊奶及其乳制品中。
现有技术中去除黄曲霉毒素B1的方法有：氨化法(是美国专利方法)，该法用于含水饲料的毒素去除，有效率达98％，但因处理后食品中含大量的氨，美国FDA规定此法不能用于食品加工。NaOH法(曾用于植物油除去毒素)，用强碱把毒素变成盐，再用水洗。由于设备投资大、油耗大、成本高的缺点，现已被淘汰；混合溶剂萃取法：用一些有机溶剂如：95％乙醇溶液，80％2-丙醇，90％丙醇及多种乙烷和乙醇混合物从样品中萃取黄曲霉毒素B1，该方法用于如花生粉，棉籽等固态食品的毒素去除，效率可达93-98％，能保持产品中蛋白质的数量和质量，但在萃取、溶剂回收和处理时花费昂贵，无推广应用价值。高温法：用高压锅蒸煮污染的花生食品2小时或在含水量20％条件下将食品在100℃加热2小时；由于黄曲霉毒素B1耐热，须超过250℃高温才会分解，但过高温度同时会破坏食品中的基他营养成分，故不作为解毒的主要手段。其他如：次氯酸钠、臭氧、过氧化氢、甲醛、氯气等化学法，它们运用强烈的化学试剂破坏毒素，所以，同时也破坏了食品中的许多营养成分。超滤-渗滤法，用该法处理的全奶其AFM1含量可由最初的2.5μg/L下降到0.5μg/L，但经处理后的牛奶、脂肪、乳糖以及灰分和总固形物最高可损失23％，且该法对均相化或酸化的牛奶无效。
本发明的目的旨在克服现有技术的不足而提供一种方法专一、高效、温和、安全，能够较好地保持食品原有品质的黄曲霉毒素B1解毒酶去除黄曲霉毒素B1的方法。
本发明的目的是这样实现的：
一种黄曲霉毒素B1解毒酶去除黄曲霉毒素B1的方法，其特征是将黄曲霉毒素B1解毒酶直接与含毒样品作用，体系呈PH7.0-8.5，或间接地先以载体固定化后再与含毒样品作用，于20-70℃作用5-120分钟。
——所述的载体可以是甲壳素、硅胶、氧化铝、琼脂珠、多孔玻璃珠等多孔材料、惰性基质、或经活化处理的惰性基质。
——所述的含毒样品可以是植物油、酱油啤酒、奶及其制品。
下面通过实施例对本发明作进一步详述：
取含毒样品10ml，加入黄曲霉毒素B1解毒酶400μg，用磷酸盐缓冲液调至体系PH7.5，在温度为40℃作用40分钟即可去除样品中的黄曲霉毒素B1。本发明的方法专一，高效，温和和安全。该解毒酶能够破坏黄曲霉毒素B1的致毒、致癌活性，能够较好地保持食品的原有品质，因而本发明可安全无毒地用于食品加工业。

Claims (3)

1、一种黄曲霉毒素B1解毒酶去除黄曲霉毒素B1的方法，其特征是将黄曲霉毒素B1解毒酶直接与含毒样品作用，体系呈PH7.0-8.5，或间接地先以载体固定化后再与含毒样品作用，于20-70℃作用5-120分钟。
2、根据权利要求1所述的黄曲霉毒素B1解毒酶去除黄曲霉毒素B1的方法，其特征是体可以是甲壳素、硅胶、氧化铝、琼脂珠、多孔玻璃珠等多孔材料、惰性基质、或经活化处理的惰性基质。
3、根据权利要求1所述的黄曲霉毒素B1解毒酶去除黄曲霉毒素B1的方法，其特征是含毒样品可以是植物油、酱油、啤酒、奶及其制品。
CN99117161A 1999-10-27 1999-10-27 黄曲霉毒素b1解毒酶去除黄曲霉毒素b1的方法 Expired - Fee Related CN1085057C (zh)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006017960A1 (fr) * 2004-08-17 2006-02-23 Guangzhou Co-Win Bioengineering Co., Ltd. Enzyme de détoxification ayant une activité consistant à transformer l'aflatoxine et gène codant pour celle-ci
CN102197861A (zh) * 2011-01-11 2011-09-28 四川大学 一株在高盐环境中除去黄曲霉毒素b1的假丝酵母菌的使用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4013004C2 (de) * 1990-04-24 1993-10-21 Elvira Schecklies Verfahren zur quantitativen Bestimmung von niedermolekularen organischen Substanzen

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006017960A1 (fr) * 2004-08-17 2006-02-23 Guangzhou Co-Win Bioengineering Co., Ltd. Enzyme de détoxification ayant une activité consistant à transformer l'aflatoxine et gène codant pour celle-ci
CN1294255C (zh) * 2004-08-17 2007-01-10 广州科仁生物工程有限公司 一种具有转化黄曲霉毒素活性的解毒酶及编码该酶的基因
US7695751B2 (en) 2004-08-17 2010-04-13 Guangzhou Co-Win Bioengineering Co., Ltd. Detoxifizyme with activity of transforming aflatoxin and the gene encodes thereof
CN102197861A (zh) * 2011-01-11 2011-09-28 四川大学 一株在高盐环境中除去黄曲霉毒素b1的假丝酵母菌的使用
CN102197861B (zh) * 2011-01-11 2012-12-19 四川大学 一株在高盐环境中除去黄曲霉毒素b1的假丝酵母菌的使用

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