CN1258435A - Fast lotus root propagating method in detoxicated seed-breeding field - Google Patents
Fast lotus root propagating method in detoxicated seed-breeding field Download PDFInfo
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- CN1258435A CN1258435A CN 99120633 CN99120633A CN1258435A CN 1258435 A CN1258435 A CN 1258435A CN 99120633 CN99120633 CN 99120633 CN 99120633 A CN99120633 A CN 99120633A CN 1258435 A CN1258435 A CN 1258435A
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Abstract
The present invention belongs to the field of vegetable nursery technology. The stem tip of lotus rooot as explant is first inoculated to MS or B5 culture medium with 6-BA or ZRs in 0.01-8.0 mg/l and NAA in 0-10 mg/l to culture for 60-80 days. Then, great amount of seedling is cultured through subculture in MS or B5 culture medium with 6-BA in 0.01-6.4 mg/l, NAA in 0-8.0 mg/l and sugar in 30-100 g/l for 30-60 days. Finally, seedling with developed root system is cultured with the transplanted test seedling in MS or B5 culture medium with 6-BA in 0.01-4.0 mg/l, NAA in 1.0-20.0 mg/l and sugar in 30-150 g/l for 20-50 days. The seedling may be further transplanted to field.
Description
A kind of lotus rhizome virus-elimination seedlings of the present invention is the method for breeding fast.Belong to the vegetable seedling production technical field, be exclusively used in the detoxifying fast breeding of lotus rhizome seedling.
Lotus rhizome (Nelumbo nucifera Gaertn) is the traditional characteristics aquatic vegetable of China's southern area, has good alimentary health-care function, also is the main foreign exchange earning vegetables of China.Adopt vegetative propagation to make the virus accumulation because of long-term.At present each producing region all takes place seriously, so causes " stiff lotus root " problem and cause kind of a property serious degradation, and root-like stock (lotus root) diminishes, and is stiff, and the product qualitative change is bad, and output seriously descends, and has influenced its foreign exchange earning and the competitiveness on the market at home thereof.Effort improves bulb quality and output, improves reproduction coefficient, and solving and producing " stiff lotus root " problem of going up at present is the task of top priority.Generally adopt annual method of all carrying out strict land for growing field crops seed selection to improve or alleviate the generation of the problems referred to above at present, but labour intensity is big, the specification requirement height, effect is not remarkable, can not really thoroughly solve above-mentioned practical problems.
Present many asexually propagated crops all use shoot tip meristem to cultivate detoxic seedling, and fast numerous virus-elimination seedlings is used production.But do not see the research report that has the lotus rhizome stem apex detoxify fast numerous so far.
The purpose of this invention is to provide a kind of lotus rhizome virus-elimination seedlings method of breeding fast, propose to obtain fast a large amount of detoxic seedlings, the healthy and strong growth of test-tube plantlet, the best culture scheme of inducing test-tube plantlet to take root in a large number is fit to factorial seedling growth, is suitable for transplanting field growing.
The method embodiment that a kind of lotus rhizome virus-elimination seedlings provided by the present invention is bred fast is as follows:
1. getting the lotus rhizome stem apex is explant, is seeded in additional after stem apex outsourcing leaf sheath is divested: MS or the B of 0.01-8.0mg/l6-BA or ZRs and 0-10mg/l NAA
5In the medium, behind the cultivation 60-80d, get test-tube plantlet band joint position and carry out successive transfer culture.
2. subculture is numerous soon: above-mentioned test-tube plantlet is transferred in additional 0.01-6.4mg/l 6-BA, 0-8.0mg/l NAA, the MS of 30-100g/l sugar or B
5In the medium, 30-60d can form a large amount of test-tube plantlets.
3. culture of rootage: above-mentioned test-tube plantlet is transferred in additional 0.01-4.0mg/l 6-BA, 1.0-20.0mg/lNAA, the MS of 30-150mg/l sugar or B
5In the medium, can form a large amount of root systems behind the 20-50d, cultivate transplantation of seedlings field survival rate and reach more than 80%.
In the above-mentioned lotus rhizome virus-elimination seedlings method for quickly breeding, pH5.6-7.2, intensity of illumination 1800-2500lx, light application time 10-14h/d, used sugar refers to sucrose, white sugar, with sucrose for well.
The present invention compared with prior art has following advantage:
1. compare with planting of present employing with ripe rhizome (lotus root) work, the present invention utilizes the lotus rhizome stem apex to be explant, set up lotus rhizome detoxifying fast breeding system, reproduction coefficient reaches 8-20, can obtain a large amount of detoxic seedlings, and can not morph, the detoxic seedling transplanting survival rate reaches more than 80%, growth potential is prosperous, can significantly reduce sowing quantity, be convenient to the seedling transportation, thereby it be low to have solved lotus rhizome sapling multiplication coefficient of the prior art, sowing quantity is big, transportation and production cost height, is unfavorable for the problem that the rapid large-area of improved seeds is applied.
With generally adopt at present since vegetative period promptly until plant lotus root the selecting and remain for a long time of continuing to carry out of gathering and kind compare, the present invention is by cultivating detoxic seedling, labour intensity is little, the duration is short, detoxification efficiency is good.
3. the lotus rhizome virus-elimination seedlings provided by the present invention method of breeding fast is applicable to various lotus root lotus kinds.
Embodiment 1:
1) acquisition of detoxification test tube plantlet: experimental cultivar is the beauty of Baoying County red (Nelumbo mucifera Gaertn).Get beauty's red shank point and be explant, after the running water flushing, with 70% ethanol surface sterilization 1 minute, divide kind with 0.1% mercuric chloride solution sterilization 15-20 again, aseptic water washing 4-5 all over after, peel off the outsourcing leaf sheath, be inoculated in the triangular flask after cutting stem apex, with MS is minimal medium, after additional 3.2mg/l 6-BA and 4.0mg/l NAA cultivate 70d, can obtain the cultivation seedling with 12 buds.
2) subculture is numerous soon: above-mentioned test-tube plantlet cutting or band joint individuality are inoculated in the MS minimal medium, and additional 2.0mg/l 6-BA, 1.0mg/l NAA, in the MS medium of 80g/l sucrose, 50d can form the cultivation seedling with 14 buds.
3) culture of rootage seedling: above-mentioned test-tube plantlet is transferred in additional 1.0mg/l 6-BA, and 6.0mg/l NAA in the MS medium of 50g/l sucrose, forms a large amount of root systems behind the 30d, cultivate transplantation of seedlings field survival rate and reach 88%.Embodiment 2:
1) acquisition of detoxification test tube plantlet: experimental cultivar is " raising lotus root No. one " (Nelumbo nucifera Gaertn).Getting and raising stem apex of lotus root is explant, after the running water flushing, with 1 fen kind of 70% ethanol surface sterilization, divides with 0.1% mercuric chloride solution sterilization 15-20 and plants, and after aseptic water washing 4-5 time, peels off the outsourcing leaf sheath, is inoculated in the triangular flask after cutting stem apex, with B
5Be minimal medium, additional 3.0mg/l ZRs and 4.0mg/l NAA behind the cultivation 80d, can obtain the cultivation seedling with 14 buds.
2) subculture is numerous soon: above-mentioned test-tube plantlet is cut into band joint individuality, be inoculated in B
5In the minimal medium, additional 8.0mg/l ZRs, 2.0mg/l NAA, in the B5 medium of 50g/l sucrose, 50d can form the cultivation seedling with 18 buds.
3) culture of rootage: above-mentioned test-tube plantlet is transferred in additional 2.0mg/l ZRs, 10mg/l NAA, the B of 90g/l sucrose
5In the medium, promptly form a large amount of root systems behind the 30d, cultivate transplantation of seedlings land for growing field crops survival rate 92%.Embodiment 3:
For the examination lotus root varieties is that Baoying County is purplish red greatly, and getting stem apex is explant, and according to above method, result of implementation is as follows behind the adjustment medium composition:
1) test-tube plantlet medium: MS+2.0mg/l 6-BA+0.5mg/l NAA; Shoot proliferation medium: MS+3.2mg/l 6-BA+2.0mg/l NAA, 50g/l sucrose; Statistics reproduction coefficient 15 behind the 40d; Root media: MS+0.4mg/l 6-BA+8.0mg/l NAA, 60g/l sucrose is taken root behind the 30d, cultivates transplantation of seedlings land for growing field crops survival rate 87%.
2) test-tube plantlet medium: MS+3.0mg/l 6-BA+2.0mg/l NAA; Shoot proliferation medium: MS+5.0mg/l 6-BA+3mg/l NAA, 60g/l white sugar, statistics reproduction coefficient 14 behind the 40d; Root media: MS+0.4mg/l 6-BA+8.0mg/l NAA80g/l white sugar, cultivate seedling rooting behind the 30d, cultivate transplantation of seedlings land for growing field crops survival rate 90%.
3) test-tube plantlet medium: MS+6.0mg/l 6-BA+3.0mg/l NAA; Shoot proliferation medium: MS+6.0mg/l 6-BA+2.0mg/l NAA, 60g/l white sugar, statistics reproduction coefficient 16 behind the 40d; Root media: MS+0.4mg/l 6-BA+18.0mg/l NAA, 80g/l white sugar is cultivated seedling rooting behind the 30d, cultivate transplantation of seedlings land for growing field crops survival rate 91%.
4) test-tube plantlet medium: B
5+ 1.0mg/l ZRs+0.5mg/l NAA; Shoot proliferation medium: B
5+ 4.0mg/lZRs+1.0mg/l NAA, 60g/l white sugar is added up reproduction coefficient behind the 40d; Root media: B
5+ 0.02mg/l6-BA+12.0mg/l NAA, 90g/l sucrose, rooting of vitro seedling behind the 30d, test-tube seedling transplanting land for growing field crops survival rate 89%.
5) test-tube plantlet medium: B
5+ 7.0mg/l ZRs+2.0mg/l NAA; Shoot proliferation medium: B
5+ 4.0mg/lZRs+1.0mg/l NAA, 60g/l white sugar, statistics reproduction coefficient 13 behind the 40d; Root media: B
5+ 0.5mg/l6-BA+10.0mg/l NAA, 90g/l sucrose, rooting of vitro seedling behind the 30d, test-tube seedling transplanting land for growing field crops survival rate 92%.
6) test-tube plantlet medium: B
5+ 3.2mg/l ZRs+2.0mg/l NAA; Shoot proliferation medium: B
5+ 6.0mg/lZRs+2.0mg/l NAA, 50g/l sucrose, statistics reproduction coefficient 15 behind the 40d; Root media: B
5+ 0.5mg/l6-BA+10.0mg/l NAA, 90g/l sucrose, rooting of vitro seedling behind the 30d, test-tube seedling transplanting land for growing field crops survival rate 93.5%.
7) test-tube plantlet medium: MS+6.0mg/l 6-BA+3.0mg/l NAA; Shoot proliferation medium: B
5+ 4.0mg/lZRs+1.0mg/l NAA, 60g/l white sugar, statistics reproduction coefficient 14 behind the 40d; Root media: MS+0.4mg/l6-BA+18.0mg/l NAA, 80g/l white sugar is cultivated seedling rooting behind the 30d, cultivate transplantation of seedlings land for growing field crops survival rate 89%.
8) test-tube plantlet medium: MS+2.0mg/l 6-BA+0.5mg/l NAA; Shoot proliferation medium: B
5+ 4.0mg/lZRs+1.0mg/l NAA, 60g/l white sugar, statistics reproduction coefficient several 13 behind the 40d; Root media: MS+0.4mg/l6-BA+16.0mg/l NAA, 80g/l white sugar behind the 30d, is cultivated seedling rooting, cultivates transplantation of seedlings land for growing field crops survival rate 93%.
9) test-tube plantlet medium: B
5+ 7.0mg/l ZRs+2.0mg/l NAA; Shoot proliferation medium: MS+5.0mg/l6-BA+30mg/l NAA, 60g white sugar, statistics reproduction coefficient 17 behind the 40d; Root media: B
5+ 0.02mg/l6-BA+12.0mg/l NAA, 90g/l sucrose, rooting of vitro seedling behind the 30d, test-tube seedling transplanting land for growing field crops survival rate 95%.
10) test-tube plantlet medium: B
5+ 1.0mg/l ZRs+0.5mg/l NAA; Shoot proliferation medium: MS+6.0mg/lZRs+3.0mg/l NAA, 60g sucrose, statistics reproduction coefficient 13 behind the 40d; Root media: MS+0.4mg/l6-BA+8.0mg/l NAA, 80g/l white sugar is cultivated seedling rooting behind the 30d, cultivation transplantation of seedlings land for growing field crops survival rate 93%/
11) test-tube plantlet medium: B
5+ 1.0mg/l ZRs+0.5mg/l NAA; Shoot proliferation medium: MS+3.0mg/lZRs+2.0mg/l NAA, 60g/l sucrose, statistics reproduction coefficient 16 behind the 40d; Root media: MS+0.4mg/l6-BA+8.0mg/l NAA, 80g/l white sugar is cultivated seedling rooting behind the 30d, cultivate transplantation of seedlings land for growing field crops survival rate 90%.
12) test-tube plantlet medium: MS+2.0mg/l 6-BA+0.5mg/l NAA; Shoot proliferation medium: B
5+ 4.0mg/l ZRs+1.0mg/l NAA, 60g/l white sugar, statistics reproduction coefficient 17 behind the 40d; Root media: MS+0.4mg/l 6-BA+12.0mg/l NAA, 80g/l sucrose is cultivated seedling rooting behind the 30d, cultivate transplantation of seedlings land for growing field crops survival rate 95%.
13) test-tube plantlet medium: MS+3.0mg/l 6-BA+2.0mg/l NAA; Shoot proliferation medium: B
5+ 6.0mg/l ZRs+2.0mg/l NAA, 50g sucrose, statistics reproduction coefficient 17 behind the 40d; Root media: B
5+ 0.5mg/l 6-BA+10.0mg/l NAA, 90g/l sucrose, rooting of vitro seedling behind the 30d, test-tube seedling transplanting land for growing field crops survival rate 95%.
14) test-tube plantlet medium: MS+3.0mg/l 6-BA+2.0mg/l NAA; Shoot proliferation medium: B
5+ 4.0mg/l ZRs+1.0mg/l NAA, statistics reproduction coefficient 15 behind the 40d; Root media: B
5+ 0.5mg/l6-BA+10.0mg/l NAA, 90g/l sucrose is cultivated seedling rooting behind the 30d, cultivate transplantation of seedlings land for growing field crops survival rate 94%.
15) test-tube plantlet medium: MS+3.0mg/l 6-BA+2.0mg/l NAA; Shoot proliferation medium: MS+3.0mg/l ZRs+2.0mg/l NAA, 60g/l sucrose, statistics reproduction coefficient 15 behind the 40d; Root media: B
5+ 0.5mg/l 6-BA+10.0mg/l NAA, 90g/l sucrose, rooting of vitro seedling behind the 30d, test-tube seedling transplanting land for growing field crops survival rate 93%.
16) test-tube plantlet medium: B
5+ 3.2mg/l ZRs+2.0mg/l NAA; Shoot proliferation medium: B
5+ 6.0mg/lZRs+2.0mg/l NAA, 50g/l sucrose, statistics reproduction coefficient 16 behind the 40d; Root media: B
5+ 0.5mg/l6-BA+10.0mg/l NAA, 90g/l sucrose, rooting of vitro seedling behind the 30d, test-tube seedling transplanting land for growing field crops survival rate 94%.
Claims (3)
1. the lotus rhizome virus-elimination seedlings method of breeding fast, implementation step is as follows:
1.1) to get the lotus rhizome stem apex be explant, is seeded in additional after stem apex outsourcing leaf sheath is divested: MS or the B of 0.01-8.0mg/l6-BA or ZRs and 0-10mg/l NAA
5In the medium, behind the cultivation 60-80d, get test-tube plantlet band joint position and carry out successive transfer culture.
1.2) subculture is numerous soon: above-mentioned test-tube plantlet is transferred in additional 0.01-6.4mg/l 6-BA, 0-8.0mg/lNAA, the MS of 30-100g/l sugar or B
5In the medium, 30-60d can form a large amount of test-tube plantlets.
1.3) culture of rootage: above-mentioned test-tube plantlet is transferred in additional 0.01-4.0mg/1 6-BA, 1.0-20.0mg/lNAA, the MS of 30-150mg/l sugar or B
5In the medium, can form a large amount of root systems behind the 20-50d, cultivate transplantation of seedlings field survival rate and reach more than 80%.
2. a kind of lotus rhizome virus-elimination seedlings according to claim 1 is the method for breeding fast, and it is characterized in that: the condition of culture in each stage is pH5.6-7.2, intensity of illumination 1800-2500lx, light application time 10-14h/d.
3. a kind of lotus rhizome virus-elimination seedlings according to claim 1 and 2 is the method for breeding fast, and it is characterized in that: used sugar refers to sucrose, white sugar, with sucrose for well.
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| CN 99120633 CN1258435A (en) | 1999-12-22 | 1999-12-22 | Fast lotus root propagating method in detoxicated seed-breeding field |
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| CN 99120633 CN1258435A (en) | 1999-12-22 | 1999-12-22 | Fast lotus root propagating method in detoxicated seed-breeding field |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318578C (en) * | 2004-11-26 | 2007-05-30 | 扬州大学 | Domestication method for lotus root group cultivating de-poisoning breeding |
| CN101138321B (en) * | 2007-08-22 | 2011-03-30 | 浙江省农业科学院 | Sugarcane detoxication tissue culturing and fast propagating method |
| CN102640705A (en) * | 2012-04-12 | 2012-08-22 | 上海孙桥现代农业联合发展有限公司 | Fast propagation method for aquatic plant lotus flowers |
| CN105325298A (en) * | 2015-11-18 | 2016-02-17 | 扬州大学 | Primary culture method of lotus root stem tip tissue |
| CN106576735A (en) * | 2016-11-16 | 2017-04-26 | 唐滨 | Strontium-rich dry lotus root planting method using tissue culture seedlings |
| CN106717777A (en) * | 2016-11-16 | 2017-05-31 | 唐滨 | Use the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling |
| CN108541591A (en) * | 2018-05-14 | 2018-09-18 | 山东组培农业发展有限公司 | A kind of lotus rhizome detoxification method for tissue culture |
| CN111557242A (en) * | 2020-05-26 | 2020-08-21 | 中国科学院武汉植物园 | Method for culturing and rapidly propagating lotus tissue culture seedlings |
-
1999
- 1999-12-22 CN CN 99120633 patent/CN1258435A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318578C (en) * | 2004-11-26 | 2007-05-30 | 扬州大学 | Domestication method for lotus root group cultivating de-poisoning breeding |
| CN101138321B (en) * | 2007-08-22 | 2011-03-30 | 浙江省农业科学院 | Sugarcane detoxication tissue culturing and fast propagating method |
| CN102640705A (en) * | 2012-04-12 | 2012-08-22 | 上海孙桥现代农业联合发展有限公司 | Fast propagation method for aquatic plant lotus flowers |
| CN105325298A (en) * | 2015-11-18 | 2016-02-17 | 扬州大学 | Primary culture method of lotus root stem tip tissue |
| CN106576735A (en) * | 2016-11-16 | 2017-04-26 | 唐滨 | Strontium-rich dry lotus root planting method using tissue culture seedlings |
| CN106717777A (en) * | 2016-11-16 | 2017-05-31 | 唐滨 | Use the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling |
| CN108541591A (en) * | 2018-05-14 | 2018-09-18 | 山东组培农业发展有限公司 | A kind of lotus rhizome detoxification method for tissue culture |
| CN111557242A (en) * | 2020-05-26 | 2020-08-21 | 中国科学院武汉植物园 | Method for culturing and rapidly propagating lotus tissue culture seedlings |
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