CN1254508A - Preparation method of natural bud inhibitor and application of natural bud inhibitor in axillary bud inhibition of flue-cured tobacco - Google Patents
Preparation method of natural bud inhibitor and application of natural bud inhibitor in axillary bud inhibition of flue-cured tobacco Download PDFInfo
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- 239000003112 inhibitor Substances 0.000 title abstract 7
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Abstract
The invention discloses a natural sprout inhibitor for inhibiting axillary buds of flue-cured tobacco, which is prepared by collecting, separating, purifying and culturing plants, selecting strains with strong biological activity and high yield, carrying out wheat sprout inhibition rapid detection, repeatedly comparing and screening high-yield strains, culturing, extracting with a solvent, concentrating and drying to obtain two sprout inhibitors of natural abscisic acid (ABA) and Botcinolide. The effective rate of the inhibitor is 89-92% when the inhibitor is used for inhibiting the axillary buds of the flue-cured tobacco, the whole processing process is safe and non-toxic, and does not contain organic synthetic components, so that the inhibitor does not pollute the environment, has no residual toxicity, is low in production cost and low in price, and can be used for preserving flowers and vegetables, regulating and controlling the growth of crops, improving the quality, increasing the yield and the like.
Description
The present invention relates to natural preparation method and the application on the flue-cured tobacco axillalry bud suppresses thereof that presses down the bud agent, belong to biological technical field.Especially natural abscisic acid S-(+)-ABA, be called for short ABA, 5-(1-hydroxyl-2,-6,6-trimethyl-4-oxo-2-cyclohexene-1-yl)-and 3-methyl-2-is suitable-and 4-is anti--pentadienoic acid, and Botcinolide, i.e. 4-hydroxyl-2-octynic acid, two kinds of metabolites (preservation day is on September 3rd, 1998) that product all is Botrytis cinerea PersrCGMCC NO.0357-1, NO.0357-2 fungi.
Tobacco leaf is big in China's many provinces cultivated area, economic benefit is obvious.But binding in the annual field management pruned, extremely tired effort, and not long-time after extracing, the wooden fork bud that makes new advances of president again.In order to improve tobacco leaf product yield, quality and to reduce the labour, country successively from buy Flumetralin (maleic Min), the Maleichydrazide (maleic hydrazide, bud enemy) that press down bud and use abroad, to remove bud logical etc., these medicines are used for field management, bud inhibition effect has their own characteristics each, but price higher (13 yuan/mu), and they all are the organic synthesis preparations, and problems such as the influence of Flue-cured tobacco Quality and residual toxicity, environmental pollution are not resolved as yet.
The objective of the invention is to overcome the deficiencies in the prior art and drawback, a kind of safety non-toxic is provided, has no side effect, free from environmental pollution, no residual hazard misgivings, imitate the high inexpensive novel bud agent that presses down.
Above-mentioned purpose is achieved in that the fungal disease sample of extensively gathering Botrytis Cinerea Persr from plant, select the typical sample of fresh symptom, carry out surface sterilization, wash with aqua sterilisa, again with separate tissue or the direct spore method of shaking, carrying out monospore separates, purifying is cultivated, then according to indoor bacterial strain screening and technological process of production method, the choice biologically active is strong, (productive rate is more than 0.8 ‰ for the high strain system of productive rate, wheat presses down the test of bud fast detecting, and extract is diluted to 1500 times of still effective bacterial strains), relatively filter out superior strain more repeatedly, productive rate 0.9 ‰～1.5 ‰, number in regular turn at last, formally bacterial classification is inserted and boil to half-mature sterilization barley corn, put into-40 ℃～-60 ℃ refrigerators and preserve standby.Medium is PDA, PLD, PDA+ citrus peel meal, cultivates 7 days at 25 ℃, adds solvent extraction, filtration, concentrate drying then and gets natural A BA and slightly take out product, be placed on preserve in-80 ℃ of refrigerators standby.Products therefrom is adopted inhibition wheat bud scale growth method, carry out giving birth to fast and survey screening, to extract 10 times, 50 times, 100 times of the product dilutions of slightly taking out to be measured in advance ... series concentration 5ml moves into respectively in the beaker that is placed with the wheat seed of handling, be advisable contrast clear water, each concentration repetition 3 times with submergence seed just, put in 26 ℃ of incubators and cultivated 5～7 days, it is strong to filter out activity, and the bacterial strain that productive rate is high is cultivated gained crude product natural abscisic acid (ABA).Analyze with column chromatography and TLC method purifying, HPLC, use IRV, UV, MS, resolve in conjunction with the HNMR instrument, determine molecular weight and structural formula, the molecular weight of natural abscisic acid is 264.3, molecular formula C
15H
20O
4, structural formula is
For fear of wastage of material, the remaining aqueous solution after the extraction is dealt with again.Method is: the remaining aqueous solution after the extraction is adjusted PH and is alkalescence, isolate solvent layer and water layer through the EtOAC extracting, again water layer is adjusted pH value, through doubly measuring solvent extraction, isolate solvent layer and water layer once more, discard water layer, with this solvent collection of separating, after water and saturated Nacl liquid are washed, anhydrous Na
2So
4Dehydration, concentrate drying, EtOAC soluble acidic fraction obtains natural abscisic acid ABA.
The molecular weight of natural abscisic acid is 264.3, molecular formula C
15H
20O
4This structure of matter has 5 characteristics: 1. main nuclear has circulus; 2. on the circulus two keys must be arranged: 3. main nuclear has a side chain; 4. have one-COOH, carboxyl and the main internuclear carbon atom that has at least: 5. main nuclear and side chain must the tool stereochemical structures.
Another kind of preparation method is: the fungal disease sample of extensively gathering Botrytis Cinerea Persr from plant, select the typical sample of fresh symptom, carry out surface sterilization, wash with aqua sterilisa, again with separate tissue or the direct spore method of shaking, carrying out monospore separates, purifying is cultivated, then according to indoor bacterial strain screening and technological process of production method, (productive rate is more than 0.8 ‰ to select the high strain system of the strong productive rate of biologically active, wheat presses down the test of bud fast detecting, extract is diluted to 1500 times of still effective bacterial strains), relatively select superior strain more repeatedly, number at last in regular turn productive rate 0.9 ‰～1.5 ‰, formally bacterial classification is inserted and boil to half-mature sterilization barley corn, put into-40 ℃～-60 ℃ refrigerators and preserve standby.Medium is PDA, and incubated at room temperature 12 days (as cultivating 6 days for 23 ℃ with cavings) adds the product of slightly taking out that solvent extraction, filtration, concentrate drying get Botcinolide then, be placed on preserve in-80 ℃ of refrigerators standby.Products therefrom is adopted inhibition wheat bud scale growth method, carry out giving birth to fast and survey screening, the product of slightly taking out to be measured that extract are in advance diluted 10 times, 50 times, 100 times ... series concentration 5ml moves into and is placed with in the beaker of the wheat seed of handling, be advisable with submergence seed just, the contrast clear water, each concentration repeats 3 times, puts in 26 ℃ of incubators and cultivates 5～7 days, filters out the active strong high bacterial strain of productive rate and comes production natural B otcinolide.Analyze with column chromatography and TLC method purifying, HPLC, use IRV, UV, MS, resolve in conjunction with the HNMR instrument, determine molecular weight and structural formula, molecular weight is 338, molecular formula C
20H
34O
8, structural formula is
Press down the axillalry bud that the bud agent is used for the flue-cured tobacco cultivation process and suppress natural, adopt and smear or the spray mode, reached the overgrowing of inhibition cigarette bud, received good effect.Should press down the bud agent also can be used for binding of other plant and prunes.Flowers, fresh-keeping of vegetables, the regulation and control crop growth improves quality and improves output etc.
Natural press down the bud agent since be from plant, gather, separate, cultivate, extraction, filter, drying gets, whole process safety non-toxic, do not contain organic synthetic composition, thereby free from environmental pollution, no residual hazard, production cost is low, and low price, cost per mu only need 8～9 yuan, can promote Flue-cured tobacco Quality again, bud inhibition effect is on average more than 90%.Should press down the bud agent also can be used for binding of other plant and prunes.Flowers, fresh-keeping of vegetables, the regulation and control crop growth improves quality and improves output etc.
The invention will be further described below in conjunction with drawings and Examples
Fig. 1 is the natural preparation method's process chart that presses down the bud agent
Fig. 2 is the extraction process flow chart of natural abscisic acid ABA remaining aqueous solution
Fig. 3 is natural but bud agent (press down 1, press down 2) is used schematic diagram with control group in the field
At first from each plant species of natural world (fruit tree, vegetables, flowers, grain, economic crops, wild forest ... Deng) the middle fungal disease sample of gathering Botrytis Cinere Persr, select fresh, the typical sample of symptom then, carry out surface sterilization, wash with aqua sterilisa, carry out monospore separation, purifying cultivation, adopt wheat to press down the bud test method(s), the bacterial strain of screening biologically active, repeated screening is relatively sought the highest bacterial strain of active substance output capacity and is preserved as bacterial classification from activated strains again.For example, 7 bacterial strains that we are separated to from the gray mold of plants such as lettuce, green onion, strawberry, acid Chinese quince according to preceding method separation and Culture, extraction, concentrated, can obtain thick product, provide to give birth to test and test.Medium is PDA, PLD, PDA+ citrus peel meal, cultivates 7 days at 25 ℃, adds solvent extraction, filtration, concentrate drying then and gets natural A BA and slightly take out product, be placed on preserve in-80 ℃ of refrigerators standby.Products therefrom is adopted inhibition wheat bud scale growth method, carry out giving birth to fast and survey screening, the standard and the product of slightly taking out to be measured that prepare are in advance diluted 10 times, 50 times, 100 times ... series concentration 5ml moves into and is placed with in the beaker of the wheat seed of handling, be advisable with submergence seed just, the contrast clear water, each concentration repeats 3 times, puts in 26 ℃ of incubators and cultivates 5～7 days, filters out the active strong high natural abscisic acid (ABA) of productive rate.Analyze with column chromatography and TLC method purifying, HPLC, use IRV, UV, MS, in conjunction with the parsing of HNMR instrument, molecular weight and structural formula, the molecular weight of natural abscisic acid is 264.3, molecular formula C
15H
20O
4, structural formula is
For fear of wastage of material, the remaining aqueous solution after the extraction is dealt with again.Method is: the remaining aqueous solution after the extraction is adjusted PH and is alkalescence, isolate solvent layer and water layer through the EtOAC extracting, again water layer is adjusted pH value, through doubly measuring solvent extraction, isolate solvent layer and water layer once more, discard water layer, with this solvent collection of separating, after water and saturated Nacl liquid are washed, anhydrous Na
2So
4Dehydration, concentrate drying, EtOAC soluble acidic fraction obtains natural abscisic acid ABA.
Another kind of preparation method is: will screen the high bacterial classification of productive rate, use the PDA medium, incubated at room temperature 12 days, if make medium with cavings, cultivated 6 days for 23 ℃,, filter, concentrate then with doubly measuring the ethyl acetate lixiviate 24 hours (attention jolting), concentrate with the benzene lixiviate again, promptly obtain the natural bud agent Botcinolide of pressing down.Analyze with column chromatography and TLC method purifying, HPLC, use IRV, UV, MS, resolve in conjunction with the HNMR instrument, determine molecular weight and structural formula, molecular weight is 338, molecular formula C
20H
34O
8, structural formula is
Naturally press down the inhibition that the bud agent is used for the flue-cured tobacco axillalry bud with what these two kinds of methods were prepared.Adopt and suppress wheat bud scale growth method, carry out giving birth to fast and survey screening.Method is to get the interior moisture absorption of selected wheat seed (eliminate and remove sick the damage, be empty flat) preimpregnation 1/1000 carbendazim suspension to handle 24 hours.It is some to get sterilization beaker (high 6cm, footpath 4.5cm), and 40 in little glass ball is respectively put at the cup end, pad one deck sterilization circular filter paper sheet on it, and uniform again wheat seed is 10 on the paper.Standard that will prepare in advance and the product of slightly taking out to be measured dilute 10 times, 50 times, 100 times respectively ... series concentration 5ml moves in the beaker, be advisable with submergence seed just, the contrast clear water, each concentration repeats 4 times, put in 26 ℃ of incubators and cultivated 5～7 days, observation young root, young shoot growth have the unrestraint phenomenon, and tentatively judge whether contain active ingredient in the extracting crude product.As previously mentioned, product is identified through indoor biometrics earlier, is proved that activity is very high, press down bud preliminary examination through the strain of indoor pot cigarette again, effect is obvious, carries out the multiple spot field plot trial at last, in late June, 98～late July, select growth neat respectively in our province Xun Dian county, Luliang County, Milei County, the height unanimity, kind is identical, the vega that management is the same, after (preferably selecting big gold dollar of safflower or V-2 series of products) normally bound and pruned, treat that axillalry bud has just revealed about 1 centimeter long of point, divides the experimental plot by the field layout drawing.Test medicine: ABA (press down 1), Botcinolide (pressing down 2), maleic Min, remove that bud is logical, the bud enemy.According to experimental scheme, take by weighing a certain amount of each reagent agent respectively, add water and stir, be modulated into desired concn, ABA (pressing down 1) is diluted to 100,200,300 times; Botcinolide (pressing down 2) is diluted to 100,200 times; 300 times of maleic Mins (active ingredient 25.5%); Remove logical (active ingredient 33%) 100 times of bud; Bud enemy (active ingredient 30.2%) 50 times; The clear water contrast.Totally nine processing, four repetitions amount to 36 sub-districts, and 0.03 mu of every sub-district is with field district's group arrangement at random.Select fine the no phase on rain day, after 5 o'clock that afternoons,, handle postfixed point and list and observe record to each sub-district cigarette strain axillalry bud with cup pouring method or semar technique dispenser, bud inhibition effect 90～92%, cost per mu only need 8～9 yuan.And the domestic organic synthesis maleic Min of promoting the use of, bud enemy, to remove bud logical etc., 13 yuan of cost per mus, though bud inhibition effect all reaches 89～92%, but environmental pollution and residual hazard are still unresolved, just press down the bud effect, naturally press down the bud agent to press down the bud agent be competitive as novel, adopt and smear every mu and only need 10 grams, adopt and spray every mu 20 gram, price is also more cheap.Should press down the bud agent also can be used for binding of other plant and prunes.Flowers, fresh-keeping of vegetables, the regulation and control crop growth improves quality and improves output etc.
Claims (4)
1. natural preparation method who presses down the bud agent, it is characterized in that: a, from plant, extensively gather the disease sample of Botrytis Cinerea Persr fungi CGMCC NO.0357-1, select the typical sample of fresh symptom, carry out surface sterilization, wash with aqua sterilisa, again with separate tissue or the direct spore method of shaking, carrying out monospore separates, purifying is cultivated, then according to indoor bacterial strain screening and technological process of production method, (productive rate is more than 0.8% to select the high strain system of the strong productive rate of biologically active, wheat presses down the test of bud fast detecting, and extract is diluted to 1500 times of still effective bacterial strains), relatively select superior strain more repeatedly, productive rate 0.9 ‰～1.5 ‰, number in regular turn at last, formally bacterial classification is inserted and boil to half-mature sterilization barley corn, put into-40 ℃～-60 ℃ refrigerators and preserve standby.B, medium is PDA, PLD, the PDA+ citrus peel meal, cultivated 7 days at 25 ℃, add solvent extraction then, filter, concentrate drying gets natural A BA and slightly takes out product, it is standby to be placed on the interior preservation of-80 ℃ of refrigerators, c, products therefrom is adopted inhibition wheat bud scale growth method, carry out giving birth to fast and survey screening, the standard A BA liquid and the product of slightly taking out to be measured that prepare are in advance diluted 10 times, 50 times, 100 times ... series concentration 5ml moves into respectively in the beaker that is placed with the wheat seed of handling, be advisable with submergence seed just, the contrast clear water, each concentration repeats 3 times, put in 26 ℃ of incubators and cultivated 5～7 days, filter out activity by force, the strain culturing gained crude product natural abscisic acid (ABA) that productive rate is high, d, use column chromatography and TLC method purifying again, HPLC analyzes, use IRV, UV, MS resolves in conjunction with the HNMR instrument, determines molecular weight and structural formula, the molecular weight of natural abscisic acid is 264.3, molecular formula C
15H
20O
4, structural formula is
2. natural preparation method who presses down the bud agent, it is characterized in that: a, from plant, extensively gather the disease sample of Botrytis Cinerea Persr fungi CGMCC NO.0357-2, select the typical sample of fresh symptom, carry out surface sterilization, wash with aqua sterilisa, again with separate tissue or the direct spore method of shaking, carrying out monospore separates, purifying is cultivated, then according to indoor bacterial strain screening and technological process of production method, (productive rate is more than 0.8% to select the high strain system of the strong productive rate of biologically active, wheat presses down the test of bud fast detecting, extract is diluted to 1500 times of still effective bacterial strains), relatively select superior strain more repeatedly, productive rate 0.9 ‰～1.5 ‰, number in regular turn at last, formally bacterial classification is inserted and boil to half-mature sterilization barley corn, putting into-40 ℃～-60 ℃ refrigerators preserves standby, b, medium is PDA, incubated at room temperature 12 days (as cultivating 6 days for 23 ℃ with cavings) adds solvent extraction then, filter, concentrate drying gets the product of slightly taking out of Botcinolide, it is standby to be placed on the interior preservation of one 80 ℃ of refrigerators, c, products therefrom is adopted inhibition wheat bud scale growth method, carry out giving birth to fast and survey screening, the product of slightly taking out to be measured that extract are in advance diluted 10 times, 50 times, 100 times ... series concentration 5ml moves into and is placed with in the beaker of the wheat seed of handling, be advisable with submergence seed just, the contrast clear water, each concentration repeats 3 times, put in 26 ℃ of incubators and cultivated 5～7 days, filter out the active strong high bacterial strain of productive rate and come production natural B otcinolide, d, use column chromatography and TLC method purifying again, HPLC analyzes, use IRV, UV, MS resolves in conjunction with the HNMR instrument, determines molecular weight and structural formula, molecular weight is 338, molecular formula C
20H
34O
8, structural formula is
3. according to claim 1 or the 2 described natural bud agent that press down, be used for the flue-cured tobacco axillalry bud and suppress.
4. the described and preparation method according to claim 1, it is characterized in that: the remaining aqueous solution after the extraction is adjusted PH and is alkalescence, isolate solvent layer and water layer through the EtOAC extracting, again water layer is adjusted pH value,, isolate solvent layer and water layer once more through doubly measuring solvent extraction, discard water layer, with this solvent collection of separating, after water and saturated Nacl liquid are washed, anhydrous Na
2So
4The dehydration, concentrate drying, EtOAC handle the soluble acidic fraction, obtain natural abscisic acid ABA.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98122743A CN1076587C (en) | 1998-11-21 | 1998-11-21 | Preparation method of natural bud inhibitor and application of natural bud inhibitor in axillary bud inhibition of flue-cured tobacco |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98122743A CN1076587C (en) | 1998-11-21 | 1998-11-21 | Preparation method of natural bud inhibitor and application of natural bud inhibitor in axillary bud inhibition of flue-cured tobacco |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1254508A true CN1254508A (en) | 2000-05-31 |
| CN1076587C CN1076587C (en) | 2001-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98122743A Expired - Fee Related CN1076587C (en) | 1998-11-21 | 1998-11-21 | Preparation method of natural bud inhibitor and application of natural bud inhibitor in axillary bud inhibition of flue-cured tobacco |
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| Country | Link |
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| CN (1) | CN1076587C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783087A (en) * | 2014-02-21 | 2014-05-14 | 重庆迎龙化工厂 | Preparation method for novel suckercide |
| CN105439847A (en) * | 2015-12-23 | 2016-03-30 | 江西新瑞丰生化有限公司 | Separation purification method for natural abscisic acid |
| CN114642206A (en) * | 2022-03-29 | 2022-06-21 | 贵州省烟草公司贵阳市公司 | Tobacco bud inhibitor suspending agent, preparation method and application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5455221A (en) * | 1994-04-29 | 1995-10-03 | The United States Of America As Represented By The Secretary Of Agriculture | Botcinolide: a natural product herbicide which is a hydroxylated monalactone |
| JPH06312975A (en) * | 1993-04-30 | 1994-11-08 | Toray Ind Inc | Method for isolating abscisic acid |
| JPH0776570A (en) * | 1993-09-08 | 1995-03-20 | Toray Ind Inc | Isolation of abscisic acid |
| CN1067724C (en) * | 1996-11-18 | 2001-06-27 | 中国科学院成都生物研究所 | Method for producing natural abscisic acid by fungus fermentation |
-
1998
- 1998-11-21 CN CN98122743A patent/CN1076587C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783087A (en) * | 2014-02-21 | 2014-05-14 | 重庆迎龙化工厂 | Preparation method for novel suckercide |
| CN103783087B (en) * | 2014-02-21 | 2017-01-25 | 重庆迎龙化工厂 | Preparation method for suckercide |
| CN105439847A (en) * | 2015-12-23 | 2016-03-30 | 江西新瑞丰生化有限公司 | Separation purification method for natural abscisic acid |
| CN114642206A (en) * | 2022-03-29 | 2022-06-21 | 贵州省烟草公司贵阳市公司 | Tobacco bud inhibitor suspending agent, preparation method and application thereof |
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| Publication number | Publication date |
|---|---|
| CN1076587C (en) | 2001-12-26 |
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