CN1251759A - Pure pallas pit viper powder and its preparing method - Google Patents
Pure pallas pit viper powder and its preparing method Download PDFInfo
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- CN1251759A CN1251759A CN99116807A CN99116807A CN1251759A CN 1251759 A CN1251759 A CN 1251759A CN 99116807 A CN99116807 A CN 99116807A CN 99116807 A CN99116807 A CN 99116807A CN 1251759 A CN1251759 A CN 1251759A
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- pit viper
- pallas pit
- viper powder
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- powder
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Abstract
A pure Pallas pit viper powder is prepared from only Pallas pit viper through selecting raw material, washing, low-temp vacuum drying, mechanical pulverizing, microwave sterilization and filling in capsules. It can promote metabolism and appetitis, improve physical strength, memory and immunity, relieve fatigue, delay sanility, beautify face and suppress growth of cancer cells.
Description
The present invention relates to a kind of health food and manufacture method thereof.
Along with improving constantly of living standards of the people, people more and more are concerned about the health status of penetrating certainly and pay attention to, thereby also more and more higher to the requirement of nutritional health food.At present, the snake powder kind on the market is quite a lot of, but because the kind of Serpentis is many, and various Serpentis class has various nutrition and health care effect to human body, the nutrition health-care functions that therefore mixes snake powder compares relatively poor.
The objective of the invention is: a kind of pure pallas pit viper powder and manufacture method thereof are provided, and it is the snake powder made from the single variety Agkistrodon halys, has higher nutritive value and health-care effect preferably.
Technical scheme of the present invention is;
A kind of pure pallas pit viper powder, this pallas pit viper powder all is processed into by Agkistrodon halys, does not add other material.
A kind of manufacture method of pure pallas pit viper powder comprises the following steps:
(1) select the high-quality Agkistrodon halys for use, remove and decaptitate and internal organs, rinsing is clean;
(2) Agkistrodon halys after will cleaning carries out low-temperature vacuum drying;
(3) mechanical activation comminution and sieve fine powder, stir;
(4) pallas pit viper powder is carried out microwave sterilizating;
(5) pallas pit viper powder after the sterilization is packed into capsule.
Advantage of the present invention is:
1. Agkistrodon halys is the Viperidae animal, is the dietotherapeutic product, and is sweet in flavor and warm in property nontoxic, the merit of strengthening by means of tonics is arranged, inspiring enthusiasm.Among the people, with being analeptic, analeptic, make powder, when when overwork, cold disease, can take, burn black sustainability pulverizing and can be used for knife injury and suppurative lump outward, cure mainly all wind insensitive impediment, diseases such as apathy.
2. contain protein, fat, taurine, each seed amino acid (comprising glutamic acid, aspartic acid, glycine, alanine, valine, leucine, isoleucine, phenylalanine, tyrosine, serine, threonine, cystine, methionine, arginine, histidine, proline, tryptophan) in the composition of Agkistrodon halys.
3. this pure pallas pit viper powder has functions such as enhancing metabolism, adjust appetite, physical strength reinforcing, enhancement memory, resisting fatigue, slow aging, guarantor's youth, caring skin, raising immunocompetence, anticancer growth, and the four seasons sutable for men, women, and children are edible.
The invention will be further described below in conjunction with embodiment:
Embodiment: a kind of pure pallas pit viper powder, this pallas pit viper powder all is processed into by Agkistrodon halys, does not add other material.
A kind of manufacture method of pure pallas pit viper powder comprises the following steps:
(1) select the high-quality Agkistrodon halys for use, remove and decaptitate and internal organs, rinsing is clean;
(2) Agkistrodon halys after will cleaning carries out low-temperature vacuum drying, and temperature is controlled at below 80 ℃;
(3) mechanical activation comminution and sieve fine powder, stir;
(4) pallas pit viper powder is carried out microwave sterilizating;
(5) pallas pit viper powder after the sterilization is packed into capsule.
Modern clinical research proves that Agkistrodon halys can be treated following all diseases: the feeble child of (1) strumosis, collar lymph or pulomonary hilar lymphoid tuberculosis; (2) after being ill or women's puerperal asthenia, anemia, neuralgia, crural paralysis, flaccidity are weak, stride difficulty etc.; (3) natural pond stomach spasm; (4) control feeble child's proctoptosis, women's metroptosis; (5) control allergic constitution, skin suppurative diseases; (6) migratory arthralgia numbness, the insane disease of strong wind; (7) tuberculosis of bone and joint; (8) rheumatoid arthritis; (9) leukopenia etc.
The health food function assessment survey report of by Nanjing Medical University's nutrition and Food Science technical research institute products made thereby of the present invention being done again illustrates effect of the present invention below.
One. the pallas pit viper powder capsule is to the regulating action of immunologic function:
1. materials and methods:
1.1 given the test agent:
The pallas pit viper powder capsule is provided by Suzhou Longbao Bioengineering Co, and every contains pallas pit viper powder 0.25g.
1.2 experimental animal:
Kunming mouse, body weight 20--25g is provided by Jiangsu Province's Experimental Animal Center.The quality certification number: No. 95035, (matter) are moved by No. 95100, Soviet Union's moving (ring) and Soviet Union.
1.3 dosage grouping:
According to pallas pit viper powder capsule human intaking amount (0.25g * 2--4 grain * 2 time/day/people, be equivalent to the 25mg/kg body weight), respectively by its 2.5 times 10 times 40 times of three dosage groups of design and negative control group, be 62.5mg/kg, 250mg/kg, 1000mg/kg and distilled water matched group, irritate stomach every day once, continuous 30 days.
1.4 test method:
Undertaken by " the health food function assessment assessment process and the method for inspection ".
1.4.1 dinitrofluorobenzene (DNFB) inducing mouse (ear swelling method)
1.4.1.1 grouping reaches to sample:
Get the male mice in kunming of body weight 20--25g, be divided into four groups at random, every group more than 10, gavaging the high, medium and low dosage of pallas pit viper powder respectively is 62.5mg/kg, 250mg/kg, 1000mg/kg and feminine gender (distilled water) matched group, once a day, and continuous 30 days.
1.4.1.2 sensitization:
Every Mus skin of abdomen loses hair or feathers with barium sulfide, the about 3cm * 3cm of scope, and 50ul evenly smears sensitization with 1%DNFB solution.
1.4.1.3 attack:
Evenly smear in the mouse right ear two sides with 1%DNFB solution 10ul after 5 days and attack.
1.4.1.4 measure:
Attack the dislocation of back 24 hours cervical vertebras and put to death mice, cut left and right sides auricular concha, take off the auricle of diameter 8mm, weigh with card punch.
1.4.1.5 date processing:
Represent the degree of DTH with the difference of left and right sides ear weight, adopt variance analysis to carry out data statistics.
1.4.2 serum hemolysin is measured:
1.4.2.1 grouping reaches to sample:
Get body weight 20--25g male mice in kunming, be divided into four groups at random, every group more than 10, grouping and to the same 1.4.1.1 of quadrat method.
1.4.2.2SRBC and complement preparation:
Undertaken by the method for inspection.
1.4.2.3 immune animal and serum separate:
Every Mus lumbar injection 2%SRBC suspension 0.2ml plucks eyeball after 4 days and gets blood, separation of serum.
1.4.2.4 hemolytic reaction:
After 250 times of dilutions of serum, the optical density value during by method of inspection working sample pipe and SRBC HD50.
1.4.2.5 date processing:
The amount of hemolysin is with half hemolysis value (HC
50) expression, carry out data statistics with variance analysis.
1.4.3 mice carbon clearance test:
1.4.3.1 grouping reaches to sample:
Get body weight 20--25g male mice in kunming, be divided into four groups at random, every group more than 10, grouping and to the same 1.4.1.1 of quadrat method.
1.4.3.2 measure:
Every caudal vein injects the india ink (0.1ml/10g body weight) of five times of dilutions, injects and gets blood 20ul from the angular vein clump respectively in back 2 minutes and 10 minutes, and it is added to 2mlNa
2CO
3In the solution, use spectrophotometer at 600nm wavelength place's photometry density value.Simultaneously mice is put to death, get liver and spleen is weighed.
1.4.3.3 date processing:
Represent that with gauged phagocytic index a mice carbon cleans up ability, carry out data statistics with variance analysis.
1.4.4 internal organs/body weight ratio:
1.4.4.1 grouping reaches to sample:
Same 1.4.3.1.
1.4.4.2 measure:
Last gave behind the sample 24 hours to put to death mice, weighed, and got thymus, spleen and weighed.
1.4.4.3 date processing:
Carry out data statistics with variance analysis.
2. result
2.1 the pallas pit viper powder capsule is to the influence of DNFB inducing mouse DTH:
Table 1 is the result show, middle dosage group DTH is apparently higher than matched group, and both difference significances (P＜0.05) show that middle dosage group pallas pit viper powder capsule has the effect that strengthens DTH.
Table 1 pallas pit viper powder capsule is to the influence of DNFB inducing mouse DTH
Group dosage (mg/kg.BW) number of animals (only) auricular concha weightening finish (mg)
Matched group 0 10 5.9 ± 4.0
Low dose group 62.5 10 5.5 ± 2.3
Middle dosage group 250 10 10.4 ± 5.3
*
High dose group 1,000 10 5.6 ± 1.6
Annotate: " * " compares P＜0.05 with matched group.
2.2 the influence that the pallas pit viper powder capsule forms the mice serum hemolysin:
By table 2 result as seen, high, middle dosage group HC
50Apparently higher than matched group, illustrate that the pallas pit viper powder capsule has facilitation to the formation of mice serum hemolysin.
The influence that table 2 pallas pit viper powder capsule forms the mice serum hemolysin
Group dosage (mg/kgBW) number of animals (only) HC
50
Matched group 0 10 28.9 ± 26.6
Low dose group 62.5 10 43.9 ± 55.3
Middle dosage group 250 10 57.1 ± 35.0
*
High dose group 1,000 10 71.4 ± 28.1
*
Annotate: " * " compares P＜0.05 with matched group." * * " compares P＜0.01 with matched group.
2.3 the influence that the pallas pit viper powder capsule acts on clearly mice carbon corridor:
Table 3 result shows that the pallas pit viper powder capsule does not still have obviously influence of generation to clear the acting in mice carbon granules corridor.
Table 3 pallas pit viper powder capsule is to the influence of the clear effect in mice carbon corridor
Group dosage (mg/kgBW) number of animals (only) phagocytic index a
Dosage group 250 10 5.4475 ± 0.7173 high dose group 1,000 10 4.9096 ± 0.6299 in matched group 0 10 4.8639 ± 0.5919 low dose group 62.5 10 4.2453 ± 1.0544
2.4 the pallas pit viper powder capsule is to the influence of immune organ
As can be seen from Table 4, pallas pit viper powder capsule height, middle dosage group mouse thymus/body weight ratio illustrate the effect that the pallas pit viper powder capsule has increases mouse thymus weight apparently higher than matched group (P＜0.01).
Table 4 pallas pit viper powder capsule is to the influence of immune organ
Group dosage (mg/kgBW) number of animals (only) thymus/body weight (mg/g) spleen/body weight (mg/g)
Matched group 0 10 1.56 ± 0.73 6.67 ± 1.32
Low dose group 62.5 10 2.20 ± 1.48 5.20 ± 1.64
Middle dosage group 250 10 3.00 ± 1.00
*7.00 ± 1.73
High dose group 1,000 10 3.50 ± 0.76
*6.50 ± 3.25
Annotate: " * * " compares P＜0.01 with matched group.
3. conclusion
The pallas pit viper powder capsule is given mouse stomach 30 days with 62.5mg/kg, 250mg/kg, 1000mg/kg dosage, the result shows that 250mg/kg dosage group can strengthen the effect of mouse DTH, and 250mg/kg, 1000mg/kg dosage group all can promote the formation of hemolysin and the weightening finish of thymus.Therefore think that the pallas pit viper powder capsule has immunization.
Two. the capsular antifatigue effect of pallas pit viper powder
1. materials and methods:
1.1 given the test agent:
The pallas pit viper powder capsule is provided by Suzhou Longbao Bioengineering Co, and every contains pallas pit viper powder 0.25g.
1.2 experimental animal:
Male mice in kunming, body weight are 20--25g, are provided by Jiangsu Province's Experimental Animal Center.The quality certification number: No. 95035, (matter) are moved by No. 95100, Soviet Union's moving (ring) and Soviet Union.
1.3 dosage grouping:
According to the actual intake of pallas pit viper powder capsule human body (0.25g * 2--4 grain * 2 time/day/people, be equivalent to the 25mg/kg body weight), respectively by its 2.5 times 10 times 40 times of three dosage groups of design and negative control group, be 62.5mg/kg, 250mg/kg, 1000mg/kg and distilled water matched group, irritate stomach every day once, continuous 30 days.
1.4 test method;
Undertaken by " the health food function assessment assessment process and the method for inspection ".
1.4.1 swimming with a load attached to the body test:
1.4.1.1 grouping reaches to sample:
Male mice in kunming is divided into four groups at random, and every group more than 10, gavaging the high, medium and low dosage of pallas pit viper powder respectively is 62.5mg/kg, 250mg/kg, 1000mg/kg and feminine gender (distilled water) matched group, once a day, and continuous 30 days.
1.4.1.2 test:
Last gives sample after 30 minutes, and the bear a heavy burden sheet lead of 5% body weight of every Mus is put in the water tank of 25 ℃ of water temperatures, depth of water 30--40cm and swum, the record mice from the swimming beginning to the dead time, as mice swimming time (min).
1.4.1.3 date processing:
Carry out data statistics with variance analysis.
1.4.5 blood creatinine is measured:
1.4.5.1 grouping reaches to sample:
Same 1.4.1.1.
1.4.5.2 measure:
Last is given behind the sample 30 minutes, and swimming stopped after 90 minutes in temperature 25--30 ℃ water, plucked the eyeball blood sampling after quiet 15 minutes.Get 0.2m1 serum, press serum creatinine and measure (not removing protein method) test kit description mensuration.Test kit is provided by Shanghai Rongsheng Bioisystech Co., Ltd.
1.4.5.3 date processing:
Same 1.4.1.3.
2. result:
2.1 the pallas pit viper powder capsule is to the influence of mouse movement endurance:
Table 5 is the result show, pallas pit viper powder capsule height, middle dosage group mice swimming time are apparently higher than matched group, and both differences have significance (P＜0.05) respectively, shows that the pallas pit viper powder capsule has the effect that strengthens mouse movement endurance.
Table 5 pallas pit viper powder capsule is to the influence of mice swimming time
Group dosage (mg/kgBW) number of animals (only) swimming time (min)
Matched group 0 10 15.6 ± 23.0
Low dose group 62.5 10 25.1 ± 18.4
Middle dosage group 250 10 57.5 ± 44.1
High dose group 1,000 10 49.8 ± 37.5
*
Annotate: " * " compares P＜0.05 with matched group.
2.2 the pallas pit viper powder capsule is to the influence of motion biochemical indicator:
By table 6 result as can be seen, low, the middle dosage group of pallas pit viper powder capsule blood lactate level is starkly lower than knot according to group (P＜0.05, P＜0.01), and high, medium and low dosage group liver glycogen content is apparently higher than matched group (P＜0.05, P＜0.01).
Table 6 pallas pit viper powder capsule is to the influence of mouse movement biochemical indicator
Group dosage blood lactic acid serum urea nitrogen hepatic glycogen kreatinin hemoglobin
(mg/kgBW) (mol/L) (mmol/L) (mg%) (umol/L) (g/dl) matched group 0 6.21 ± 1.68 6.87 ± 1.04 32.9 ± 37.6 92.9 ± 52.9 13.3 ± 2.9 low dose group 62.5 4.71 ± 1.07
*6.82 ± 2.35 95.4 ± 69.6
*131.2 dosage group 250 4.18 ± 0.62 in ± 56.7 14.6 ± 1.6
*6.05 ± 1.01 101.9 ± 60.2
*140.6 ± 72.2 14.2 ± 2.3 high dose group 1,000 5.57 ± 0.98 6.02 ± 2.26 81.5 ± 50.2
*100.5 ± 38.4 13.9 ± 0.8 annotate: " * " compares with matched group, P＜0.05, and " * * " compares P＜0.01 with matched group
3. conclusion:
The pallas pit viper powder capsule is given mouse stomach 30 days with 62.5mg/kg, 250mg/kg, 1000mg/kg dosage, the result shows: 250mg/kg, 1000mg/kg dosage group have the effect that improves mouse movement endurance, and 62.5mg/kg, 250mg/kg dosage group can reduce Mouse Liver glycogen content.Therefore the pallas pit viper powder capsule has antifatigue effect.
Claims (3)
1. pure pallas pit viper powder, it is characterized in that: this pallas pit viper powder all is processed into by Agkistrodon halys, does not add other material.
2. the manufacture method of a pure pallas pit viper powder is characterized in that: comprise the following steps:
(1) select the high-quality Agkistrodon halys for use, remove and decaptitate and internal organs, rinsing is clean;
(2) Agkistrodon halys after will cleaning carries out low-temperature vacuum drying;
(3) mechanical activation comminution and sieve fine powder, stir;
(4) pallas pit viper powder is carried out microwave sterilizating.
3. the manufacture method of pure pallas pit viper powder according to claim 2 is characterized in that: also comprise the following steps:
(5) pallas pit viper powder after the sterilization is packed into capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99116807A CN1251759A (en) | 1999-08-20 | 1999-08-20 | Pure pallas pit viper powder and its preparing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99116807A CN1251759A (en) | 1999-08-20 | 1999-08-20 | Pure pallas pit viper powder and its preparing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1251759A true CN1251759A (en) | 2000-05-03 |
Family
ID=5279483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99116807A Pending CN1251759A (en) | 1999-08-20 | 1999-08-20 | Pure pallas pit viper powder and its preparing method |
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| Country | Link |
|---|---|
| CN (1) | CN1251759A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104306403A (en) * | 2014-09-29 | 2015-01-28 | 郭德志 | Pure snake powder and preparation method thereof |
| CN115444866A (en) * | 2022-08-23 | 2022-12-09 | 广西金圣堂生物医药科技有限公司 | Application of cobra powder or water extract thereof in preparation of anti-liver cancer drugs |
-
1999
- 1999-08-20 CN CN99116807A patent/CN1251759A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104306403A (en) * | 2014-09-29 | 2015-01-28 | 郭德志 | Pure snake powder and preparation method thereof |
| CN115444866A (en) * | 2022-08-23 | 2022-12-09 | 广西金圣堂生物医药科技有限公司 | Application of cobra powder or water extract thereof in preparation of anti-liver cancer drugs |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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