CN1243836A

CN1243836A - Process for preparing chitin from abacterial fly maggot

Info

Publication number: CN1243836A
Application number: CN 98103446
Authority: CN
Inventors: 司信
Original assignee: Individual
Current assignee: Individual
Priority date: 1998-07-30
Filing date: 1998-07-30
Publication date: 2000-02-09

Abstract

A process for preparing chitin from the a bacterial fly maggot includes such steps as abacterial culture of fly maggot, squeezing, water washing, drying, pulverizing to obtain powdered fly maggot skin, decalcifying with 1 mol/L hydrochloric acid, washing in water until it shows neutral, removing protein and fat with 5% sodium hydroxide in water bath at 100 deg.C, washing in water until it becomes neutral, repeating said acid and alkali treatments, and drying at 100 deg.C for 3 hr to obtain high-purity chitin. The used acid and alkali obtained inthe process are neutralized.

Description

Method with preparing chitin from abacterial fly maggot

At insect world, the fecundity of fly ranks first place, and the fly blow of the aseptic fly kind product of propagating artificially every day was cultivated larva after three days, and output is very big.Larva not only contains protein, and contains the fine chitin in the maggot skin.The chitosan that chitin obtains behind deacetylation can be widely used in industries such as environment protection, food_beverage industry, medicines and health protection, daily-use chemical industry.Yet do not see the technology of announcing with preparing chitin from abacterial fly maggot so far as yet, influence the development and use of aseptic fly-maggot.

The objective of the invention is to invent a kind of technology with preparing chitin from abacterial fly maggot.

Step of the present invention is as follows:

1, selecting to propagate artificially the larva that aseptic fly cultivates is aseptic fly-maggot, and it is very big to propagate the aseptic fly-maggot quantity of being produced artificially, all can cultivate throughout the year, and therefore selecting to propagate artificially the larva that aseptic fly cultivates is aseptic fly-maggot.

2, the aseptic fly-maggot of cultivating pushed, clean, dry, pulverize and be dried maggot skin powder.Method is aseptic fly-maggot to be put into squeezing machine push, wait to remove thing such as internal organ after, with flushing with clean water three times.The maggot skin is put into dryer dry, bake out temperature is controlled at 100 ℃, 3 hours time, and the maggot skin after the oven dry is put into the pulverizer of 30 mesh sieves and pulverized, make dried maggot skin powder.

3, the dried maggot skin powder hydrochloric acid soln decalcification in reactor that will make, clean, drain.Method is that dried maggot skin powder is put into pure stainless steel reaction pot, is that the hydrochloric acid soln of 1mol/L is poured in the reactor with compound concentration, soaks 20 minutes, and the hydrochloric acid consumption is 3 times of calcium carbonate content in the maggot skin, is washed till neutrality with distilled water then, drains.

4, the maggot skin powder sodium hydroxide solution in reactor after the decalcification is carried out deproteinization with fat, clean, drain.Method is that the maggot skin powder after the decalcification is put into pure stainless steel reaction pot, is that 5% sodium hydroxide solution is poured in the reactor with compound concentration, 100 ℃ of boiling water baths heating of reactor external application, reaction times is 1 hour, leach product, be washed till neutrality with distilled water then, drain.

5, product is further handled.Method is to repeat 2 hydrochloric acid solns to handle and the sodium hydroxide solution processing, is washed till neutrality with distilled water then, drains.

6, product is carried out drying treatment, make chitin.Method is that the neutral products that obtains through three hypo acids, alkaline purification is put into dryer, and temperature is 100 ℃, 3 hours time, gets the high-quality chitin.

7, pour spent acid solution, waste lye into the neutralization reaction pond and carry out neutralization reaction, wastewater is discharged in obtaining.

Present method is can remove the contained inorganic salt such as lime carbonate of maggot skin according to hydrochloric acid, and sodium hydroxide can be removed the contained protein of maggot skin and grease is invented.

The contriver has made test of many times to maggot skin amount, concentration of hydrochloric acid and consumption, naoh concentration and consumption and has learnt, when concentration of hydrochloric acid is 1mol/L, content of hydrochloric acid is 3 times calciferous of a maggot skin, under the room temperature, reaction times is when being 20 minutes, can make product not contain inorganic salt, decalcification is thorough, and when naoh concentration is 5%, sodium hydroxide solution is wanted complete submergence product, reaction times is 1 hour, when the outer 100 ℃ of boiling water baths of reactor heat, can make the product deproteinization, the degreasing fat is thorough, this kind process will repeat 2 times, can obtain highly purified chitin.When carrying out deproteinization, during the degreasing fat, reactor does not have the boiling water bath heating outward, and then concentration sodium hydroxide will reach 10%, and under the room temperature, the reaction times is taken 3 days, and changes once fresh alkali lye every day, like this could deproteinize and fat.Products therefrom washes to neutrality repeatedly through distilled water, and oven dry obtains chitin, and yield is 8%, does not have ash, and nitrogen content is 7.2%.

Claims

1, utilizes fly maggot to prepare the method for chitin, it is characterized in that the larva of selecting aseptic housefly to cultivate carries out sterilizing-drying, pulverization process is made dried maggot powder, be the raw material of preparation chitin; Dried maggot powder is reacted decalcification in hydrochloric acid soln; Maggot powder after the decalcification is washed till neutrality with distilled water drains, the maggot powder after the decalcification is carried out deproteinization and degreasing fat in sodium hydroxide solution; With the product of deproteinization degreasing fat, extremely neutral earlier with distilled water, the dry chitin that gets; Spent acid solution and waste lye are carried out neutralizing treatment; To decalcification in the hydrochloric acid solution of fly maggot powder, be washed to neutrality, deproteinization and the fatty process of maggot powder in sodium hydroxide solution repeats 2 times.

2, the method with preparing chitin from abacterial fly maggot according to claim 1, the no bacterial classification fly that it is characterized in that artificial culture be through laying eggs and the hatching of fly blow, the cultivation of larva and as aseptic fly-maggot.

3, the method with preparing chitin from abacterial fly maggot according to claim 1, it is characterized in that larva through extruding, washing, dry, cross 30 mesh sieves and pulverize and form the maggot powder, drying temperature is controlled at 100 ℃, 30 minutes time.

4, the method with preparing chitin from abacterial fly maggot according to claim 1 is characterized in that concentration of hydrochloric acid solution is 1mol/L, and soaking at room temperature maggot powder 20 minutes, hydrochloric acid consumption are 3 times of lime carbonate calculated amount in the maggot skin, and distilled water is washed till neutrality, drains.

5, the method with preparing chitin from abacterial fly maggot according to claim 1 is characterized in that concentration of sodium hydroxide solution is 5%, 100 ℃ of boiling water bath heating of reactor external application, and in 1 hour reaction times, distilled water is washed till neutrality, drains.96% solid sodium hydroxide 15.7 gram adding distil waters 284.3 grams can restrain with 100 gram maggot powder prepared in reaction chitins 8.

6, the method with preparing chitin from abacterial fly maggot according to claim 1 is characterized in that the hydrochloric acid decalcification of aseptic fly-maggot, and washing is to neutral, and the deproteinization of sodium hydroxide and degreasing fat wash to the neutral process and will repeat twice.

7, the method with preparing chitin from abacterial fly maggot according to claim 1 is characterized in that the spent acid solution and the waste lye of reaction back gained are carried out the neutralization reaction processing.

8, the method with preparing chitin from abacterial fly maggot according to claim 1, it is characterized in that dried maggot powder behind decalcification, deproteinization, degreasing fat products therefrom after drying gained be chitin, 100 ℃ of bake out temperatures, the time is 3 hours.